Traffic oscillations, or simply stop-and-go waves, are a common phenomenon arising in congested traffic but still not well understood. This phenomenon causes broad adverse impacts to safety risk, fuel efficiency and greenhouse emission. To eliminate or reduce those impacts, understanding the cause and propagation mechanism is essential. This dissertation studied driving behavior in traffic oscillations with the objective to uncover the formation and propagation mechanism of traffic oscillations. This study establishes a behavioral car-following model, the Asymmetric Behavioral model, based on empirical trajectory data that is able to reproduce the spontaneous formation and ensuing propagation of traffic oscillations in congested traffic. By analyzing individual drivers' car-following behavior throughout oscillation cycles it is found that this behavior is consistent across drivers and can be captured by a simple model. The statistical analysis of the model's parameters reveals that driver' behavior during oscillation (i.e., reaction to oscillation) is strongly correlated with driver behavior before oscillations and it varies with the development stage of the oscillation. Simulation of the model shows that it is able to produce characteristics of traffic oscillations consistently with empirical observations. This study also unveils the generation mechanism of the traffic hysteresis phenomenon arising in traffic oscillations using the Asymmetric Behavioral model. It is found that the occurrence of traffic hysteresis is closely correlated with driver behavior when experiencing traffic oscillations. In the growth and fully-developed stage of traffic oscillations, drivers behave differently, which results in different distribution of hysteresis patterns. This research makes it possible to unveil new management and control strategies of traffic oscillations to improve traffic operation and to quantify the environmental and safety impacts of traffic oscillations. For example, it can be used to estimate the increase of greenhouse emission and decrease of fuel efficiency imposed by traffic oscillations. It can also be used to study the increase of accident rate.

Double-stranded RNA fungal virus Helminthosporium victoriae virus 190S (genus Victorivirus, family Totiviridae) contains two large open reading frames (ORFs) that overlap in the tetranucleotide AUGA. Translation of the downstream ORF, which encodes the RNA-dependent RNA polymerase (RdRp), was previously proposed to depend on ribosomal reinitiation following termination of the upstream ORF, which encodes the capsid protein. In this study, I provided evidence to confirm that coupled termination-reinitiation (stop-restart) is indeed used. A dual-fluorescence method was established to define the RNA sequence determinants for RdRp translation. Stop-restart depends on a 32-nt stretch of RNA sequence immediately upstream of the AUGA motif, including a predicted pseudoknot structure. The presence of similar sequence motifs and predicted RNA structures in other victoriviruses suggest that they all share a related stop–restart strategy for RdRp translation. The close proximity of the secondary structure to the AUGA motif appears to be especially important for promoting translation of the downstream ORF. Normal strong preferences for AUG start codons and canonical sequence context for translation initiation of the downstream ORF appear somewhat relaxed. With dual-fluorescence system, reinitiation efficiency of the downstream ORF was determined to be ~3.9%. Pseudoknot swapping between the one in HvV190S and those predicted from other victoriviruses showed that reinitiation from the downstream ORF of HvV190S is quite tolerant to varying primary sequences of the various pseudoknots. Mutational analysis by introducing different combinations of nucleotide mutations into pseudoknot stems reproducibly confirmed the determinant role of pseudoknot on reinitiation using two different experimental systems. Together, these results provide the first example of coupled termination-reinitiation regulated by a simple pseudoknot stucture. These data expanded the understanding of coupled termination-reinitiation mechanism employed by RNA viruses and refined a new model for genus victorivirus, the largest genus in the family Totiviridae. The dual fluorescence system used in this study represented the first application of an efficient in vivo assay for recording low-frequency events in filamentous fungi.

La protéine STOP (MAP6) associée aux microtubules joue un rôle clé dans l'architecture neuronale et la plasticité synaptique, dont les dysfonctionnements sont considérés comme étant impliqués dans la physiopathologie des maladies psychiatriques. En accord avec cette hypothèse, la délétion de la protéine STOP chez la souris, conduit à des altérations neuroanatomiques, biochimiques et comportementales, en partie atténuées par des traitements antipsychotiques. Dans cette étude, nous avons tout d'abord examiné les possibles altérations des systèmes monoaminergiques chez les souris STOP KO. Chez les souris mutantes, les marqueurs sérotoninergiques et noradrénergiques sont accumulés dans le mésencéphale et, au contraire fortement diminués dans toutes les régions de projections du cerveau antérieur. De plus, ces déséquilibres monoaminergiques sont associés à une augmentation du statut dépressif, une diminution du statut anxieux et des déficits dans des tâches d'apprentissage et de mémorisation. Les effets d'un traitement chronique par la fluoxétine ou par l'épothilone D, un composé analogue du taxol stabilisant les microtubules, sur l'humeur et les performances cognitives des souris STOP KO ont aussi été évalués. Le traitement chronique par la fluoxétine induit des effets paradoxaux sur le statut dépressif et le statut anxieux des souris STOP KO suivant les tests effectués, probablement à cause d'une hypersensibilité au stress. En revanche, les traitements chroniques à la fluoxétine et à l'épothilone D ont amélioré la mémoire à court terme des souris STOP KO. Dans l'ensemble, ces résultats indiquent que la délétion de la protéine STOP chez la souris induit de fortes altérations de l'humeur et des performances cognitives et que la protéine STOP pourrait avoir un rôle crucial dans le développement des systèmes monoaminergiques.

Actuellement le marché automobile est en forte demande de modèles hybrides capables de limiter les consommations et les rejets de CO2. Parmi les diff érentes solutions technologiques possibles, cette thèse traite d'un dispositif microhybride de type Stop-Start reposant sur l'utilisation de moteurs à aimants permanents à commutation mécanique. Ce travail de recherche, encadré par Valeo et le laboratoire de Génie Électrique de Grenoble (G2Elab), a pour vocation à élaborer des méthodologies de conception permettant une implémentation facile et à bas coût de cette fonction. L'étude se concentre sur une modélisation numérique rigoureuse de la machine électrique pour ensuite analyser plusieurs améliorations techniques du démarreur en vue d'augmenter ses performances. Pour cela nous avons développé un modèle précis et rapide de la machine grâce à une approche d'identi cation des paramètres par optimisation et en utilisant la méthode des plans d'expériences. Ce modèle nous a permis de proposer des structures de démarreurs renforcés adaptés à la fonction Stop-Start.

Le NMD (nonsense-mediated mRNA decay) est un mécanisme qui reconnaît et dégrade les ARNm portant un codon stop prématuré afin d'empêcher la synthèse de protéines tronquées qui pourraient avoir des effets néfastes pour la cellule ou tout simplement être non fonctionnelles. Cependant, dans un certain nombre de cas, selon la position du codon stop prématuré, la protéine tronquée qui serait synthétisée si le NMD n'existait pas, pourrait remplir complètement ou partiellement la fonction de la protéine sauvage. Il faut noter qu'un codon stop prématuré est retrouvé dans le gène responsable d'une pathologie dans un tiers des maladies génétiques et de nombreuses formes de cancer. Dans la plus grande majorité des cas, la maladie se développe non pas parce qu'une protéine tronquée non fonctionnelle ou instable est synthétisée, mais plutôt parce que le gène muté n'est pas exprimé du fait de l'intervention du NMD sur l'ARNm qui en dérive. Une nouvelle approche thérapeutique de ces maladies serait d'inhiber le NMD afin de permettre la synthèse de protéines tronquées fonctionnelles et sauver le phénotype clinique. Nous avons donc décidé de rechercher des inhibiteurs du mécanisme du NMD parmi des petites molécules chimiques. Pour cela, nous avons mis au point un système de criblage en culture cellulaire reliant l'efficacité du NMD dans une cellule avec une activité luciférase mesurable directement sur les cultures cellulaires, au moyen d'un luminomètre. A partir d'un premier criblage d'environ 1500 composés chimiques, nous avons identifié une nouvelle molécule capable d'inhiber efficacement le NMD. De façon intéressante, cette nouvelle molécule est capable également d'induire la synthèse de protéines entières à partir d'un ARNm portant un codon stop prématuré. Nous avons utilisé cet inhibiteur dans des expériences pour déterminer son potentiel thérapeutique sur des modèles cellulaires de maladies génétiques tels que la dystrophie musculaire de Duchenne, la mucoviscidose et le cancer. Nos résultats démontrent que l'inhibition du NMD peut être en effet envisagée comme une nouvelle approche thérapeutique pour des maladies causées par l'apparition d'une mutation non sens. Nous avons aussi identifié une autre molécule chimique capable d'inhiber le NMD et permettant de faire un lien entre efficacité du NMD et intégrité du cytosquelette.

We elaborate upon the Herty-Illner macroscopic traffic models which include special non-local forces. The first chapter presents these in relation to the traffic models of Aw-Rascle and Zhang, arguing that non-local forces are necessary for a realistic description of traffic. The second chapter considers travelling wave solutions for the Herty-Illner macroscopic models. The travelling wave ansatz for the braking scenario reveals a curiously implicit nonlinear functional differential equation, the jam equation, whose unknown is, at least to conventional tools, inextricably self-argumentative! Observing that analytic solution methods fail for the jam equation yet succeed for equations with similar coefficients raises a challenging problem of pure and applied mathematical interest. An unjam equation analogous to the jam equation explored by Illner and McGregor is derived. The third chapter outlines refinements for the Herty-Illner models. Numerics allow exploration of the refined model dynamics in a variety of realistic traffic situations, leading to a discussion of the broadened applicability conferred by the refinements: ultimately the prediction of stop-and-go waves. The conclusion asserts that all of the above contribute knowledge pertinent to traffic control for reduced congestion and ameliorated vehicular flow. / Graduate

To reduce greenhouse gas emissions in the atmosphere and protect the environment, Swedish cities are heavily invested in sustainable development by developing pedestrian roads and intensifying public transport. Their goal is to make the population less dependent on cars by facilitating accessibility to transit transport. The urban area of ​​Umeå (Sweden), which is experiencing an annual increasing of it population due to urban development, includes 244 km of pedestrian or bike routes and an intensified bus network. The purpose of this paper is to evaluate the walking accessibility of the population at bus stops and to analyze the socio-economic relationship. The use of GIS tools allowed to calculate the proportion of the population and the houses around the bus stop, the average distance accessibility. The use of 3D allowed the observation of access constraints related to the topography and to calculate the slope. The use of the multiple regression model has analyzed the relationship between the shortest accessibility distance and the socio-economic factors that are the income, gender and age. The results show that the average distance of access to the bus stop is 186m. 99.2% of the population lives within 800m from the bus stops. The results of the regression showed that income is the main factor that pushes people to take the bus and live in certain types of housing. The observation of the 3d map and the calculation of the slope made it possible to know the neighborhoods established in the hill and whose residents are susceptible to spend more energy than the other inhabitant living on flat ground. The results mean that most people living in the urban area of ​​Umeå have good access to the bus stop whatever the social group, the level of income and the type of housing.

abstract: The objective of this work is to develop a Stop-Rotor Multimode UAV. This UAV is capable of vertical take-off and landing like a helicopter and can convert from a helicopter mode to an airplane mode in mid-flight. Thus, this UAV can hover as a helicopter and achieve high mission range of an airplane. The stop-rotor concept implies that in mid-flight the lift generating helicopter rotor stops and rotates the blades into airplane wings. The thrust in airplane mode is then provided by a pusher propeller. The aircraft configuration presents unique challenges in flight dynamics, modeling and control. In this thesis a mathematical model along with the design and simulations of a hover control will be presented. In addition, the discussion of the performance in fixed-wing flight, and the autopilot architecture of the UAV will be presented. Also presented, are some experimental "conversion" results where the Stop-Rotor aircraft was dropped from a hot air balloon and performed a successful conversion from helicopter to airplane mode. / Dissertation/Thesis / M.S.Tech Mechanical Engineering 2011

Submitted by Patricia Figuti Venturini (pfiguti@anhembi.br) on 2018-10-15T20:32:09Z No. of bitstreams: 1 Marcio Rodriguez Tau.pdf: 5360355 bytes, checksum: bd4040c29dc9856f3b6ab93bdaa4c8fc (MD5) / Approved for entry into archive by Patricia Figuti Venturini (pfiguti@anhembi.br) on 2018-10-17T18:43:38Z (GMT) No. of bitstreams: 1 Marcio Rodriguez Tau.pdf: 5360355 bytes, checksum: bd4040c29dc9856f3b6ab93bdaa4c8fc (MD5) / Approved for entry into archive by Patricia Figuti Venturini (pfiguti@anhembi.br) on 2018-10-17T18:46:43Z (GMT) No. of bitstreams: 1 Marcio Rodriguez Tau.pdf: 5360355 bytes, checksum: bd4040c29dc9856f3b6ab93bdaa4c8fc (MD5) / Made available in DSpace on 2018-10-17T18:46:59Z (GMT). No. of bitstreams: 1 Marcio Rodriguez Tau.pdf: 5360355 bytes, checksum: bd4040c29dc9856f3b6ab93bdaa4c8fc (MD5) Previous issue date: 2017-02-23 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES / This dissertation investigates the use of graphic elements in the cinema, with the purpose of understanding how the design can collaborate to the construction of the audiovisual message and to identify the potentialities that the movement can add to the graphic compositions in open titles film. The project started with the realization that more and more graphic designers have the need to incorporate time and movement into their multiplatform projects, which in turn raises a number of theoretical and practical reflections. Thus, within the delimited theoretical scope, subjects related to moving image, principles of animation, digital graphic design, motion graphics and the graphic design in the cinema and open titles films were approached through authors like Arlindo Machado ( (2005 and 2010), Gilles Deleuze (1985 and 1990), Paul Wells (1998 and 2008), Donis Dondis (2007), Ellen Lupton (2013 and 2015), Matt Woolman (2004), Lev Manovich 2007, 2008 and 2012) and Gemma Soloma and Antonio Boneu (2007), among others. Finally, as a case study, a brief contextualization is presented, followed by the analyzes of the opening credits of the films: “Até que a sorte nos separe 2” (2013, direction: Roberto Santucci) and “Meu passado me condena: o filme”(2013, directed by: Julia Rezende). The proposal to choose these films was to provide a look at the contemporary production of Brazilian audiovisual graphic design, as well as to enable a study on open titles national films - not identified in the literature of the area consulted in this research / Essa dissertação investiga o uso de elementos gráficos no cinema, com o objetivo de entender como o design pode colaborar para a construção da mensagem audiovisual e identificar as potencialidades que o movimento pode acrescentar às composições gráficas em créditos de aberturas de filmes. O projeto partiu da constatação de que cada vez mais os designers gráficos tem a necessidade de incorporar o tempo e o movimento em seus projetos multiplataformas, o que suscita, por sua vez, diversas reflexões de ordem teórica e prática. Assim sendo, dentro do escopo teórico delimitado, foram abordados assuntos referentes à imagem em movimento, princípios da animação, design gráfico digital, motion graphics e o design gráfico no cinema e em créditos de abertura de filmes, por meio de autores como Arlindo Machado (1997, 2005 e 2010), Gilles Deleuze (1985 e 1990), Paul Wells (1998 e 2008), Donis Dondis (2007), Ellen Lupton (2013 e 2015), Matt Woolman (2004), Lev Manovich (2005, 2006, 2007, 2008 e 2012) e Gemma Soloma e Antonio Boneu (2007), entre outros. Por fim, é apresentado, como estudo de caso, uma breve contextualização seguida das análises dos créditos de abertura dos filmes: "Até que a sorte nos separe 2" (2013, direção: Roberto Santucci) e "Meu passado me condena: o filme" (2013, direção: Julia Rezende). A proposta de escolher esses filmes foi a de propiciar um olhar sobre a produção contemporânea do design gráfico audiovisual brasileiro, além de possibilitar um estudo sobre créditos de abertura de filmes nacionais – não identificado na literatura da área consultada nesta pesquisa.

In 2011 five days of rioting spread across many English towns and cities. David Cameron, then UK Prime Minister, described these events as ‘criminality, pure and simple’, inhibiting serious examination of what happened and justifying harsh punitive sentences for rioters. This thesis explains and counters the naïve individualism that underpins the discourse of ‘criminality’; but further argues that such discursive acts are representative of a broader problem within the social order that is causally implicated the violence in 2011. In contrast to the popular and sociological approach of analysing the singular ‘riot’, ‘riot actions’ are conceptualised as the foci of analysis, and in turn argued as acts of resistance generated by the organisation and practice of power within the social order. Thus riot actions are conceptualised and function as a symptom and entry point by which analysis can better get under the skin of the social order and understand its failing. Arguing for violent acts against the police as symptomatic of the social order’s failure, the thesis examines instances of these in the 2011 and 1980s riots. The thesis explores and compares the involvement of race, exclusion, social identity, and police during and across these periods. It further examines how neoliberal forms of exclusion have shaped the possibilities of riotous actions, before performing a situational analysis of video footage of the 2011 riots. To facilitate this approach the thesis develops a theory of action/resistance through an account of the production of agency. The theory connects Bourdieu’s theorisation of habitus and disposition, utilises an expressive understanding of shame and self-esteem, and Butler’s notion of performativity. Thus we seek to understand how structured experiences, in particular social and economic exclusion, become meaningful to those excluded, and how this shapes violent acts as meaningful performances. The thesis argues that resistance is generated through power relations, which amongst many rioters, are failing to reproduce the sense of self-worth required for identification with and engagement in, the social order. From this standpoint, then, riotous resistance cannot be explained as distinct from the social order, which shapes agency’s ‘necessary scene’, but as rational and emotional responses to it. The emergence of neoliberalism and individualism in the 1970s and 1980s created an epistemological and thus ontological shift, reshaping how disrespect and disempowerment is experienced and understood by excluded groups. These shifts or emergences have diminished the capacity of socially and economically excluded groups to generate Politicised identities and forms of resistance. Consequently, rather than ‘criminality’ - a moral condemnation - the 1980s and 2011, saw an increasing emergence of individualised - rather than Politicised - forms of resistance against the social and political order. Individualised resistance to power within the social order is ‘performed’ through short-term goals that momentarily re- arrange these power relations with regards to the self and police. In these behaviours, structurally produced shame and anger are expressed, social identities are formed and realised through a common complaint and goal, and the self achieves value through attacking or confounding the police.