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Seasonal phenology and mating frequency of moths in the genus Dioryctria found sympatrically in north Okanagan Valley, British Columbia seed orchards were assessed. Female moths in the abietella, auranticella, ponderosae and schuetzeella species groups were trapped in Douglas-fir, lodgepole pine and interior spruce stands. Most species were univoltine based on one peak of flight activity per season. There is evidence that the abietella group are bivoltine in this region. Females in the abietella and auranticella groups are polyandrous; ponderosae and schuetzeella females are monandrous. The sole abietella species, D. abietivorella, recorded in British Columbia can have substantial economic impacts on seed production in commercial seed orchards. Factors influencing reproductive behaviour, longevity and fecundity of D. abietivorella were investigated. Females are synovigenic and have an income-breeding mating strategy. Reproductive behaviours are delayed post-eclosion and signalling receptivity by females coincides with egg maturation, increasing with age. Female D. abietivorella experience trade-offs between reproduction and longevity. / Ecology

Pollen contamination is a severe problem in production breeding programs since it reduces the expected gain. In an attempt to solve this problem Skogforsk created an isolation experiment in the advanced Scots pine (Pinus sylvestris) seed orchard Västerhus in Västerbotten, Sweden. This experiment involves covering blocks of trees with a tent during the pollination period so that they only can mate with each other inside. To evaluate the effects of this tent treatment one tree from inside a tent with supplementary mass pollination (SMP) and one tree from the open control were chosen for this study. 48 seeds from each tree were sampled and genotypes at 9 microsatellite (SSR) loci. The likelihood and exclusion methods for paternity assignment were used to establish the fathers to these seeds. The results showed 0% contamination inside the tent and 4-8% outside in the control. The number of fathers contributed to the fertilization of the 48 seeds was 9 inside and 15 outside. The selfing rate was unexpectedly high, 10% inside the tent and 19% outside. The mating system inside the tent need to be further evaluated to fully understand what other effect the treatment has on the future progeny.

In this study, pollen contamination (measured by the number of seeds pollinated by pollen not belonging to any of the clones on the seed orchard) is compared between open pollination and isolated pollination environments in a Scots pine (Pinus sylvestris) seed orchard called Västerhus, located outside Önsköldsvik. In this seed orchard a tent experiment is taking place. Skogforsk has built six large tents covering 12-14 trees with each tent. Fertilization patterns were compared between one tree in a tent with a supplementation of pollen from five clones not represented in the tent and another tree of the same clone subject to open pollination outside the tents. By using DNA markers the paternity was determined for 48 seeds from each tree. The results show a big difference in pollen contamination between the two trees. The tree inside the tent showed a contamination rate of 0% and the tree from the open pollination had a contamination rate of 20,5%. No difference was found in the numbers of different fathers although the selfing rate of 4,26% was higher in the tent than the selfing rate of 2,56% observed in the open pollination environment. These results match the results of other studies done on the same orchard pretty well and therefore tent isolation with a supplementary pollination can be a good way to reduce unwanted pollen contamination.

In this study, pollen contamination (measured by the number of seeds pollinated by pollen not belonging to any of the clones on the seed orchard) is compared between open pollination and isolated pollination environments in a Scots pine (Pinus sylvestris) seed orchard called Västerhus, located outside Önsköldsvik. In this seed orchard a tent experiment is taking place. Skogforsk has built six large tents covering 12-14 trees with each tent. Fertilization patterns were compared between one tree in a tent with a supplementation of pollen from five clones not represented in the tent and another tree of the same clone subject to open pollination outside the tents. By using DNA markers the paternity was determined for 48 seeds from each tree. The results show a big difference in pollen contamination between the two trees. The tree inside the tent showed a contamination rate of 0% and the tree from the open pollination had a contamination rate of 20,5%. No difference was found in the numbers of different fathers although the selfing rate of 4,26% was higher in the tent than the selfing rate of 2,56% observed in the open pollination environment. These results match the results of other studies done on the same orchard pretty well and therefore tent isolation with a supplementary pollination can be a good way to reduce unwanted pollen contamination.

Orientador: Mario Luiz Teixeira de Moraes / Resumo: As ações antrópicas no bioma Cerrado levaram a perturbações do seu ecossistema e a fragmentação das florestas, colocando em risco de extinção a espécie Myracrodruon urundeuva. O objetivo do estudo foi avaliar os caracteres silviculturais para estimar os parâmetros genéticos nos bancos de germoplasma provenientes de populações naturais em Bauru-SP e Selvíria-MS, a fim de adquirir informações que possibilitem a formação de pomares de sementes melhoradas visando a qualidade da madeira. Aos 31 anos de idade, analisou-se os caracteres altura total (ALT), diâmetro médio da copa (DMC), diâmetro na altura do peito (DAP), forma do fuste (FOR), densidade básica da madeira (DBM), relação cerne/alburno (RC/Ab) e sobrevivência (SOB). Utilizou-se o procedimento REML/BLUP via metodologia dos modelos lineares mistos para estimar os componentes de variância, os parâmetros genéticos e as estimativas de correlações genéticas entre os caracteres. Para as análises de divergência genética foi utilizado o programa Genes. Os testes de progênies de ambas as populações apresentaram variação genética. Os caracteres referentes a qualidade de madeira (DBM e RC/Ab) foram os que apresentaram maiores herdabilidades, com cerca de 0,80 e coeficiente de variação genética (16,57% a 70,10%). As correlações genéticas mostraram diferentes resultados em ambas as populações, com Selvíria sendo a população com maiores correlações positivas significativas, permitindo o uso de seleção direta e indireta tanto para os ca... (Resumo completo, clicar acesso eletrônico abaixo) / Abstract: Anthropic actions in the Cerrado biome led to disturbances of its ecosystem and the fragmentation of forests, putting the species Myracrodruon urundeuva at risk of extinction. The objective of the study was to evaluate the silvicultural traits to estimate the genetic parameters in the germplasm banks from natural populations in Bauru-SP and Selvíria-MS, in order to acquire information that allows the formation of improved seed orchards aiming at the quality of the wood. At 31 years of age, the characters total height (ALT), average crown diameter (DMC), diameter at chest height (DAP), stem shape (FOR), basic wood density (DBM), ratio heartwood / sapwood (RC / Ab) and survival (SOB). The REML / BLUP procedure was used via mixed linear models methodology to estimate the components of variance, genetic parameters and estimates of genetic correlations between the characters. For the genetic divergence analyzes, the Genes program was used. The progeny tests of both populations showed genetic variation. The characters referring to wood quality (DBM and RC / Ab) were those with the highest heritability, with about 0,80 and coefficient of genetic variation (16,57% to 70,10%). The genetic correlations showed different results in both populations, with Selvíria being the population with the highest significant positive correlations, allowing the use of direct and indirect selection for both growth and wood quality characters. In the dissimilarity analyzes, the Selvíria population was t... (Complete abstract click electronic access below) / Mestre

Submitted by Camila Motta Marin Bernardi (camilamottamarinbernardi@yahoo.com.br) on 2018-06-26T11:17:49Z No. of bitstreams: 1 BERNARDI_Camila_dr_2018.pdf: 3698349 bytes, checksum: 0e2aa782c8b7790334ec9ecc10d8b3d1 (MD5) / Approved for entry into archive by Cristina Alexandra de Godoy null (cristina@adm.feis.unesp.br) on 2018-06-26T16:58:16Z (GMT) No. of bitstreams: 1 bernardi_cmm_dr_ilha.pdf: 3698349 bytes, checksum: 0e2aa782c8b7790334ec9ecc10d8b3d1 (MD5) / Made available in DSpace on 2018-06-26T16:58:16Z (GMT). No. of bitstreams: 1 bernardi_cmm_dr_ilha.pdf: 3698349 bytes, checksum: 0e2aa782c8b7790334ec9ecc10d8b3d1 (MD5) Previous issue date: 2018-04-27 / Neste estudo objetivou-se estimar parâmetros genéticos e estatísticos para caracteres quantitativos nos primeiros seis anos de crescimento de progênies de Eucalyptus grandis em sistema silvipastoril; comparar a produtividade, estabilidade e adaptabilidade das progênies em diferentes idades e mensurar a produção de forragem e as características morfológicas e bromatológicas do capim Urochloa decumbens cv. Basilisk em crescimento livre no sub-bosque das árvores. Foram mensurados a altura (ALT), diâmetro a altura do peito (DAP) e sobrevivência (SOB), aos 2, 3, 4, 5, e 6 anos após o plantio. O delineamento utilizado foi o de blocos casualizados desbalanceados com 150 tratamentos (147 progênies de Eucalyptus grandis e três clones comerciais: C041H, P4295H e H15), 26 repetições e uma planta por parcela, no espaçamento de 2,0 x 2,5 x 4,0 m. Para avaliação do capim foram coletadas amostras da área abaixo e entre as linhas duplas de E. grandis. Na análise individual, os clones comerciais tiveram praticamente 100% de seus indivíduos entre os primeiros 250 classificados, as progênies 25 e 162 apresentaram número significativo de indivíduos nesta classificação. Em relação a classificação dos genitores, ao longo dos anos, os clones C041H e H15 sempre ficaram entre as primeiras posições. Mas as progênies 25 e 162 superaram o clone P4295H nos caracteres ALT, DAP e VOL, com correlação alta e positiva entre os anos para ALT e DAP (>0,74). Houve diferença significativa entre os tratamentos no decorrer dos anos de avaliação para os caracteres mensurados. Na análise conjunta para estabilidade, adaptabilidade e produtividade, as progênies 25, 162, 163 e 6 ficaram muito próximas a materiais comerciais (H15 e C041H) e tiveram maior estabilidade, adaptabilidade e produtividade em relação ao clone testemunha P4295H para o caráter DAP. A correlação entre os valores genéticos aditivos e de dominância para os caracteres ALT e DAP foram altos (>0,77). A produção de massa seca total de (MST) (645 kg ha-1) de capim e de folhas (272 kg ha-1) foram significativamente maiores (p<0,05) nas áreas menos sombreadas, entre as fileiras duplas e, valores nutritivos importantes, como a fração protéica e a digestibilidade, não apresentam grandes variações entre os tratamentos. Os resultados mostram ser possível continuar o programa de melhoramento genético com formação do pomar de sementes por mudas e teste de segunda geração, pois existem materiais com grande potencial de produção no local de estudo para futuros cruzamentos e formação de novos genótipos de E. grandis. / The objective of this study was to estimate genetic and statistical parameters for quantitative traits in the first six years of growth of Eucalyptus grandis progenies in the silvipastoril system; to compare productivity, stability and adaptability of progenies at different ages and to measure forage production and morphological and bromatological characteristics of Urochloa decumbens cv. Basilisk in free growth in the understorey of the trees. Height (ALT), diameter at breast height (DAP) and survival (SOB) were measured at 2, 3, 4, 5, and 6 years after planting. The experimental design was a randomized block with 150 treatments (147 progenies of Eucalyptus grandis and three commercial clones: C041H , P4295H and H15), 26 replicates and one plant per plot, at a spacing of 2.0 x 2.5 x 4 , 0 m. For evaluation of the grass samples were collected from the area below and between the double lines of E. grandis. In the individual analysis, commercial clones had practically 100% of their individuals among the first 250 classified, progenies 25 and 162 presented a significant number of individuals in this classification. Regarding the classification of the parents, over the years, the clones C041H and H15 always were among the first positions. But progenies 25 and 162 outperformed clone P4295H in ALT, DAP and VOL, with high and positive correlation between the years for ALT and DAP (> 0.74). There was a significant difference between the treatments during the evaluation years for the characters measured. In the joint analysis for stability, adaptability and productivity, progenies 25, 162, 163 and 6 were very close to commercial materials (H15 and C041H) and had greater stability, adaptability and productivity in relation to control clone P4295H for the DAP character. The correlation between the additive and dominance genetic values for the ALT and DAP characters was high (> 0.77). The total dry matter yield (MSM) (645 kg ha-1) of grass and leaves (272 kg ha-1) were significantly higher (p <0.05) in the less shaded areas between the double rows and, Important nutritional values, such as protein fraction and digestibility, do not show large variations between treatments. The results show that it is possible to continue the genetic improvement program with the seed orchard formation by seedlings and second generation test because there are materials with great potential of production in the study site for future crosses and formation of new genotypes of E. grandis.

Orientador: Miguel Luiz Menezes Freitas / Resumo: Neste estudo objetivou-se estimar parâmetros genéticos e estatísticos para caracteres quantitativos nos primeiros seis anos de crescimento de progênies de Eucalyptus grandis em sistema silvipastoril; comparar a produtividade, estabilidade e adaptabilidade das progênies em diferentes idades e mensurar a produção de forragem e as características morfológicas e bromatológicas do capim Urochloa decumbens cv. Basilisk em crescimento livre no sub-bosque das árvores. Foram mensurados a altura (ALT), diâmetro a altura do peito (DAP) e sobrevivência (SOB), aos 2, 3, 4, 5, e 6 anos após o plantio. O delineamento utilizado foi o de blocos casualizados desbalanceados com 150 tratamentos (147 progênies de Eucalyptus grandis e três clones comerciais: C041H, P4295H e H15), 26 repetições e uma planta por parcela, no espaçamento de 2,0 x 2,5 x 4,0 m. Para avaliação do capim foram coletadas amostras da área abaixo e entre as linhas duplas de E. grandis. Na análise individual, os clones comerciais tiveram praticamente 100% de seus indivíduos entre os primeiros 250 classificados, as progênies 25 e 162 apresentaram número significativo de indivíduos nesta classificação. Em relação a classificação dos genitores, ao longo dos anos, os clones C041H e H15 sempre ficaram entre as primeiras posições. Mas as progênies 25 e 162 superaram o clone P4295H nos caracteres ALT, DAP e VOL, com correlação alta e positiva entre os anos para ALT e DAP (>0,74). Houve diferença significativa entre os tratamentos no ... (Resumo completo, clicar acesso eletrônico abaixo) / Abstract: The objective of this study was to estimate genetic and statistical parameters for quantitative traits in the first six years of growth of Eucalyptus grandis progenies in the silvipastoril system; to compare productivity, stability and adaptability of progenies at different ages and to measure forage production and morphological and bromatological characteristics of Urochloa decumbens cv. Basilisk in free growth in the understorey of the trees. Height (ALT), diameter at breast height (DAP) and survival (SOB) were measured at 2, 3, 4, 5, and 6 years after planting. The experimental design was a randomized block with 150 treatments (147 progenies of Eucalyptus grandis and three commercial clones: C041H , P4295H and H15), 26 replicates and one plant per plot, at a spacing of 2.0 x 2.5 x 4 , 0 m. For evaluation of the grass samples were collected from the area below and between the double lines of E. grandis. In the individual analysis, commercial clones had practically 100% of their individuals among the first 250 classified, progenies 25 and 162 presented a significant number of individuals in this classification. Regarding the classification of the parents, over the years, the clones C041H and H15 always were among the first positions. But progenies 25 and 162 outperformed clone P4295H in ALT, DAP and VOL, with high and positive correlation between the years for ALT and DAP (> 0.74). There was a significant difference between the treatments during the evaluation years for the ... (Complete abstract click electronic access below) / Doutor

Orientador: Mario Luiz Teixeira de Moraes / Resumo: O êxito dos programas de melhoramento florestal requer que a predição do progresso genético seja proporcional ao progresso genético real. Para isso, é possível unir a genética quantitativa com ferramentas moleculares, que permitem estimar parâmetros com maior acuidade para seleção. O objetivo foi verificar a diversidade genética, o sistema de reprodução e as relações de parentesco em populações de polinização aberta de Eucalyptus urophylla, para selecionar cruzamentos e genitores. Para tanto, foram utilizados dois Pomares de Sementes por Mudas (PSM) e uma amostra de indivíduos adultos em um teste de progênies (PCPN). Para compor um teste de progênies de segunda geração (TP2G) foram coletadas sementes em 23 árvores matrizes em um dos PSM. As populações estão localizadas na Fazenda de Ensino, Pesquisa e Extensão, em Selvíria-MS. Os indivíduos do TP2G (722) e os adultos (467) foram genotipados com 12 marcadores microssatélites (SSR) e os locos avaliados para verificar se são marcadores genéticos. Os caracteres silviculturais foram mensurados nos PSM e no TP2G. Os marcadores SSR formaram um conjunto consistente de marcadores genéticos. A diversidade genética das populações foi elevada e a redução foi pequena entre as gerações. O índice de fixação foi significativo para todas as populações e maior nos descendentes, a indicar a presença de endogamia. A taxa de cruzamento indicou que a reprodução ocorreu predominantemente por cruzamentos (0,964). Houve cruzamentos entre parentes, co... (Resumo completo, clicar acesso eletrônico abaixo) / Abstract: The success of forest breeding programs requires that the prediction of genetic progress be proportionate to actual genetic progress. For this, it is possible to join the quantitative genetics with molecular tools, which allow to estimate parameters with greater acuity for selection. The main was to verify the genetic diversity, mating system and kinship relationships in open pollinated populations of Eucalyptus urophylla, to select crosses and parents. In order to do, two seedlings seed orchards (SSO) and an adult sample of individuals were used in a progeny test (PCPN). To compose a second generation progenies test (2GPT) seeds were collected in 23 mother trees in one of the SSO. The populations are located in the Fazenda de Ensino, Pesquisa e Extensão, in Selvíria-MS. The individuals from 2GTP (722) and adults (467) were genotyped with 12 microsatellite markers (SSR) and the loci evaluated for genetic markers. Silvicultural traits were measured in the SSO and 2GPT. SSR markers formed a consistent set of genetic markers. The genetic diversity of the populations was high and the reduction was small between generations. The fixation index was significant for all populations and higher in the offspring, indicating the presence of inbreeding. The multilocus estimate of outcrossing rate indicated that reproduction occurred predominantly by crosses (0.964). There were crosses between relatives, crosses correlated and self-fertilization. As a result, inbreeding in the progenies wa... (Complete abstract click electronic access below) / Doutor

Submitted by Silvelise Pupin null (silvelise.pupin@gmail.com) on 2018-02-26T14:04:43Z No. of bitstreams: 1 Tese_SilvelisePupin_VersãoFinal.pdf: 4478878 bytes, checksum: e608d55d4107cfa94a50f52defea2fad (MD5) / Approved for entry into archive by Cristina Alexandra de Godoy null (cristina@adm.feis.unesp.br) on 2018-02-26T14:25:50Z (GMT) No. of bitstreams: 1 pupin_s_dr_ilha.pdf: 4478878 bytes, checksum: e608d55d4107cfa94a50f52defea2fad (MD5) / Made available in DSpace on 2018-02-26T14:25:50Z (GMT). No. of bitstreams: 1 pupin_s_dr_ilha.pdf: 4478878 bytes, checksum: e608d55d4107cfa94a50f52defea2fad (MD5) Previous issue date: 2018-02-02 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) / O êxito dos programas de melhoramento florestal requer que a predição do progresso genético seja proporcional ao progresso genético real. Para isso, é possível unir a genética quantitativa com ferramentas moleculares, que permitem estimar parâmetros com maior acuidade para seleção. O objetivo foi verificar a diversidade genética, o sistema de reprodução e as relações de parentesco em populações de polinização aberta de Eucalyptus urophylla, para selecionar cruzamentos e genitores. Para tanto, foram utilizados dois Pomares de Sementes por Mudas (PSM) e uma amostra de indivíduos adultos em um teste de progênies (PCPN). Para compor um teste de progênies de segunda geração (TP2G) foram coletadas sementes em 23 árvores matrizes em um dos PSM. As populações estão localizadas na Fazenda de Ensino, Pesquisa e Extensão, em Selvíria-MS. Os indivíduos do TP2G (722) e os adultos (467) foram genotipados com 12 marcadores microssatélites (SSR) e os locos avaliados para verificar se são marcadores genéticos. Os caracteres silviculturais foram mensurados nos PSM e no TP2G. Os marcadores SSR formaram um conjunto consistente de marcadores genéticos. A diversidade genética das populações foi elevada e a redução foi pequena entre as gerações. O índice de fixação foi significativo para todas as populações e maior nos descendentes, a indicar a presença de endogamia. A taxa de cruzamento indicou que a reprodução ocorreu predominantemente por cruzamentos (0,964). Houve cruzamentos entre parentes, correlacionados e autofecundação. Em função disso, a endogamia nas progênies foi superior a população parental e a principal razão foi o cruzamento entre parentes, o que resultou em maior endogamia. Além disso, as relações de parentesco foram superiores no TP2G. Houve fluxo de pólen entre as populações e o número de doadores de pólen foi elevado pela análise de paternidade, que permitiu determinar o provável candidato a pai de 713 indivíduos do TP2G. Com isso foi possível estudar o teste de progênies de polinização aberta como um teste de irmãos completos, permitindo estimar as capacidades gerais e específicas de combinação. Para o diâmetro a altura do peito o ganho genético baseado no valor genotípico do cruzamento foi elevado (> 20%), assim como a seleção clonal, que indicou ganhos consideráveis (> 65%). Assim, é possível dar continuidade ao Programa de Melhoramento, indicando cruzamentos e genótipos promissores de E. urophylla. / The success of forest breeding programs requires that the prediction of genetic progress be proportionate to actual genetic progress. For this, it is possible to join the quantitative genetics with molecular tools, which allow to estimate parameters with greater acuity for selection. The main was to verify the genetic diversity, mating system and kinship relationships in open pollinated populations of Eucalyptus urophylla, to select crosses and parents. In order to do, two seedlings seed orchards (SSO) and an adult sample of individuals were used in a progeny test (PCPN). To compose a second generation progenies test (2GPT) seeds were collected in 23 mother trees in one of the SSO. The populations are located in the Fazenda de Ensino, Pesquisa e Extensão, in Selvíria-MS. The individuals from 2GTP (722) and adults (467) were genotyped with 12 microsatellite markers (SSR) and the loci evaluated for genetic markers. Silvicultural traits were measured in the SSO and 2GPT. SSR markers formed a consistent set of genetic markers. The genetic diversity of the populations was high and the reduction was small between generations. The fixation index was significant for all populations and higher in the offspring, indicating the presence of inbreeding. The multilocus estimate of outcrossing rate indicated that reproduction occurred predominantly by crosses (0.964). There were crosses between relatives, crosses correlated and self-fertilization. As a result, inbreeding in the progenies was higher than in the parental population and the main reason was cross between relatives. In addition, kinship relations were higher in 2GPT. There was pollen flow among the populations and the number of pollen donors was higher by paternity analysis, which allowed to determine the probable candidate father of 713 individuals of the 2GPT. Thus, it was possible to study the open pollinated progeny test as a full sib test, allowing the estimation of general and specific combining abilities. For the diameter at breast height, the genetic gain based on the genotypic value of the cross was high (> 20%), as well as the clonal selection, which indicated considerable gains (> 65%). Thus, it is possible to continue the Breeding Program, indicating promising crosses and genotypes of E. urophylla. / FAPESP: 2014/03407-7