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Avaliação dos patótipos de Escherichia coli circulantes no rebanho bovino e identificação das cepas de STEC isoladas no estado de São PauloSpina, Thiago Luiz Belém [UNESP] 11 September 2015 (has links) (PDF)
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000866878.pdf: 819016 bytes, checksum: f4b074f87450614f386b1be1ec42f77b (MD5) / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / A carne bovina pode ser um importante veículo de vários patógenos para os humanos, com destaque à Escherichia coli produtora de Shiga-toxina (STEC), associada com diarreia em animais e humanos. Neste estudo, investigou-se em bovinos abatidos no estado de São Paulo, a prevalência dos diferentes patótipos de E. coli diarreiogênica e o perfil de virulência dos isolados de STEC. De um total de 431 animais, STEC foi identificada em 116 (26,9%) amostras de fezes, das quais 111 (25,8%) STEC eae- e 5 (1,2%) STEC eae+. O patótipo EPEC foi detectado em 20 (4,6%) amostras de fezes dos animais testados. Os demais patótipos de E. coli diarreiogênica não foram identificados. Dos 95 isolados de STEC analisados quanto ao perfil de virulência, todos albergavam stx2, enquanto que 28 (29,5%) continham stx1. Os genes iha e saa, que codificam adesinas, foram encontrados em 93,7% (89/95) e 66,3% (63/95), respectivamente. O gene espP, que codifica uma protease que auxilia na colonização intestinal, foi detectado em 61,1% (58/95) e a hemolisina ehxA em 54,7% (52/95). Também foram identificados em menores frequências os genes subAB, nleE e nleB. STEC está amplamente disseminada nos rebanhos bovinos de São Paulo, carreando genes comumente isolados de patógenos humanos, o que reforça a importância da inspeção e fiscalização nos abatedouros / Beef can be an important vehicle for various pathogens to humans, especially Shiga toxin-producing Escherichia coli (STEC), associated to human and animal diarrhea. In this study, the prevalence of different pathotypes of diarrheagenic E. coli, and virulence profiles of STEC were investigated among feces of cattle slaughtered in São Paulo state, southeast of Brazil. From a total of 431 animals, STEC was identified from 116 (26,9%) samples, being 111 (25,8%) STEC eae- and 5 (1,1%) STEC eae+. EPEC pathotype was detected among 20 (4,6%) of animals. The other pathotypes of diarrheagenic E. coli were not identified. Of the 95 STEC isolates assessed for virulence profile, all harbored stx2, while 28 (29,5%) contained stx1. Iha and saa, genes encoding adhesins, were found at 93,7% (89/95) and 66,3% (63/95), respectively. EspP, gene which encoding a protease related with intestinal colonization, was detected in 61,1% (58/95) and ehxA hemolysin was present in 54,7% (52/95). SubAB, nleE and nleB genes were also detected in lower rates. STEC is widespread in cattle herds of São Paulo, containing commonly isolated genes from human pathogens, which reinforces the importance of inspection and surveillance in the slaughterhouses
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Regulation Of Innate Immune Cell Response Under Sub-acute/Chronic Inflammatory ConditionsNiu, Shuo 08 August 2017 (has links)
Sub-acute/chronic inflammatory diseases are often associated with altered inflammatory response, leading to increased host vulnerability to secondary inflammatory challenges. In the first study, by employing streptozotocin (STZ)-induced diabetes in mice, we further investigate mechanisms leading to enhanced polymorphonuclear leukocytes (PMN) response under hyperglycemia. We show that existence of a proinflammatory state associated with broad increases of macrophages in various organs plays a dominant role in promoting PMN response in diabetic mice. Studies of PMN infiltration during zymosan-induced peritonitis reveal that hyperglycemia enhances PMN recruitment through increasing F4/80+ macrophages in the peritoneal cavity. Insulin reversal of hyperglycemia reduces peritoneal macrophage numbers and ameliorates PMN infiltration. Significantly increased macrophages are also observed in the liver, kidneys, and intestines under hyperglycemia, and are attributable to exacerbated nephropathy and colitis when respective inflammatory conditions are induced. We also find that significant monocytosis of inflammatory F4/80+Gr-1+ monocytes from the spleen and macrophage proliferation in situ synergistically contribute to the increased macrophage population under hyperglycemia. In conclusion, our results demonstrate that STZ-induced hyperglycemic/diabetic mice develop a systemic proinflammatory state mediated by broad infiltration of macrophages. In the second study, we focus on the identification of the carrier that binds to and delivers Shiga toxin 2(Stx2) to the target organ causing hemolytic uremic syndrome (HUS). By employing a murine HUS model through co-injection of LPS-Stx2, we show that, adoptive transfer of CD11b+ leukocytes, but not CD11b- leukocytes, RBC, platelets or plasma, isolated from mice with HUS induces HUS in healthy recipients. Interestingly, we find that LPS priming of mice significantly promotes CD11b+ leukocytes binding to Stx2. Compared to CD11b+ leukocytes from mice without LPS priming, CD11b+ leukocytes isolated from mice after LPS priming demonstrate higher frequencies of toxin binding and augmented potency to induce HUS. In sum, our results demonstrate peripheral CD11b+ myeloid leukocytes act as effective Stx2 carriers that deliver toxin to kidneys causing HUS and that LPS-induced inflammation enhances the carrier capacity and aggravates HUS.
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Synthetic glycans for toxin and pathogen detectionYosief, Hailemichael 22 October 2013 (has links)
No description available.
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Cellular And Molecular Mechanisms Of Toxin Resistance For Endoplasmic Reticulum Translocating ToxinsMassey, Christopher 01 January 2009 (has links)
The endoplasmic reticulum (ER) is the site of co- and post-translational modification for secretory proteins. In order to prevent vesicular transport and secretion of misfolded or misassembled proteins, a highly regulated mechanism called ER-associated degradation (ERAD) is employed. This pathway recognizes misfolded proteins in the ER lumen and targets them to the cytosol for ubiquitination and subsequent degradation via the 26S proteasome. Sec61 and Derlin-1 are ER pores through which export occurs. AB-type protein toxins such as cholera toxin (CT), Shiga toxin (ST), exotoxin A (ETA), and ricin have evolved means of exploiting the ERAD pathway in order to reach their cytosolic targets. AB-type protein toxins consist of a catalytic A-subunit and a cell-binding B-subunit. The B-subunit recognizes cell surface receptors for the toxin. This begins a series of vesicle trafficking events, collectively termed retrograde trafficking, that lead to the ER. Dissociation of the A and B subunits occurs in the ER, and only the A subunit enters the cytosol. The exact mechanism of A subunit translocation from the ER to the cytosol is unknown. Toxin translocation occurs through a pore in the ER membrane. Exit through the pore requires the toxin to be in an unfolded conformation. The current model for toxin translocation proposes that ER chaperones actively unfold the toxin A chain for translocation. After the translocation event, the toxin spontaneously refolds to an active conformation. Our model suggests that unfolding in the ER is spontaneous and refolding in the cytosol is dependent upon cytosolic chaperones. Based on our model, we hypothesize that blockage of the A subunit unfolding and/or the ERAD translocation step will confer a phenotype of non-harmful multi-toxin resistance to cells. In support of this model, we have shown that, at 37[degrees]C, the isolated catalytic subunit of cholera toxin (CTA1) is in an unfolded and protease sensitive confirmation that identifies the toxin as misfolded by the ERAD pathway. Stabilization of CTA1 via glycerol inhibits the loss of its tertiary structure. This stabilization results in decreased translocation from the ER to the cytosol and increased secretion of CTA1 to the extracellular medium. Treatment with glycerol also prevents CTA1 degradation by the 20S proteasome in vitro. These data indicate that the thermal stability of CTA1 plays an important role in intoxication. These data also suggest that stabilization of CTA1 tertiary structure is a potential target for therapeutic agents. Our model asserts that CTA1 behaves as a normal ERAD substrate upon dissociation from the holotoxin. In support of this model, we have shown that the ER luminal protein HEDJ, known to be involved in ERAD, interacts with CTA1. The interactions between HEDJ and CTA1 occur only at temperatures in which the toxin is in an unfolded conformation. We have also shown that HEDJ does not affect the thermally stability of CTA1 since there is no alteration in its pattern of temperature-dependent protease sensitivity. Alteration of the normal HEDJ-CTA1 interaction via a dominant-negative HEDJ construct resulted in decreased translocation from the ER to the cytosol and, as a result, decreased intoxication. Our work demonstrated toxin resistance can result through effects on toxin structure or ERAD chaperones. To identify other potential inhibitors, we developed a novel assay to detect the activity of other AB toxins and compared it with an established toxicity assay. We generated a Vero cell line that expressed a destabilized variant of enhanced green fluorescent protein (EGFP). These cells were used to monitor the Stx-induced inhibition of protein synthesis by monitoring the loss of EGFP fluorescence from cells. We screened a panel of 13 plant compounds, and indentified grape seed extract and grape pomace extract as inhibitors of Stx activity. Grape seed extract and grape pomace extract were also shown to block the toxic activities of ETA and ricin, providing the basis for a future high-throughput screen for multi-toxin inhibitors.
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Structural and signaling aspects of Shiga toxinKarve, Sayali 05 June 2015 (has links)
No description available.
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Antibiotic Therapy in the Treatment of E. coli O157:H7McGannon, Colleen M. 17 April 2009 (has links)
No description available.
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Hypothesis of a Non-SNARE-Function of Syntaxin-5 / Hypothèse d'une fonction non-SNARE de la syntaxine-5Rathjen, Stefan 12 December 2017 (has links)
L’introduction commence avec la description de toxines d’origines bactérienne et végétale, en particulier la toxine Shiga ainsi que les toxines de la même famille (chapitre 9.1.2). Les petites molécules inhibitrices de ces toxines sont ensuite résumées dans le chapitre 9.1.3, en particulier le composé Retro-2. L’efficacité de ces toxines à atteindre leurs cibles reposant sur le trafic intracellulaire, un aperçu général de l’endocytose et du trafic endosomal sont présentés (chapitre 9.2). Puis, l’entrée de la voie rétrograde est décrite (chapitre 9.2.5), avec un intérêt particulier porté sur la clathrine, le rétromère et GPP130, une protéine qui circule de manière continue entre le Golgi, la membrane plasmique et les endosomes. Les protéines SNARE, en particulier la syntaxine-5 et le syntaxine-16, sont ensuite introduites (chapitre 9.2.6). Après une brève section sur les micro-ARNs de la famille 199 (chapitre 9.3), l’introduction se termine avec la description des techniques clés utilisées au cours de mon travail, tels que la chimie click bio-orthogonale, la synchronisation du trafic antérograde par rétention grâce à des hameçons spécifiques (RUSH), et la ligation par proximité basé sur des anticorps (chapitre 9.4).Ci-inclus, mon article en cours de soumission ouvre la partie résultats (chapitre 10.1), dans laquelle je présente l’intérêt de la chimie click bio-orthogonale pour identifier les cibles cellulaires de Retro-2. Je décris un des candidats potentiels, Sec16A, et illustre comment grâce à la technique de RUSH, perturber la fonction de Sec16A conduit à la relocalisation partielle de la syntaxin-5 au niveau du reticulum endoplasmique via l’inhibition du transport antérograde de la syntaxine-5. La seconde partie de l’article décrit comment la relocalisation de la syntaxine-5 induit l’inhibition du trafic de la toxine Shiga des endosomes au TGN. Je présente une nouvelle interaction entre la syntaxine-5 et la protéine TGN GPP130, qui ont déjà été caractérisées en relation avec le trafic de la toxine Shiga. Mon travail connecte à la fois les facteurs de trafic avec le trafic rétrograde au niveau de l’interface endosome-TGN. De manière frappante, cette interaction est très probablement basée sur une fonction non-SNARE de la syntaxine-5 car le domaine de fixation sur GPP130 est structurellement non lié à toute fonction SNARE.En collaboration avec Juan Francisco Aranda et Carlos Fernandez aux Etats-Unis, nous avons placés des micro-ARNs dans un contexte de régulation endogène du trafic rétrograde de la toxine Shiga (chapitre 11.2). Une discussion plus approfondie sera apportée dans le chapitre 12.Enfin, une vue d’ensemble des projets en cours est apportée dans la section des perspectives (chapitre 12), dans laquelle les collaborations plus approfondies sont mises en lumière.Mots clés : transport rétrograde, toxine Shiga, toxine de la famille Shiga, STxB, syntaxin-5, Sec16A, GPP130, Retro-2, Retro-2.1, chimie click sans cuivre, identification des cibles de petites molécules, spétrométrie de masse, function non-SNARE, inhibition du trafic antérograde, miARN, miR199, rétromère, VPS26 / The introduction of my PhD manuscript starts with describing plant and bacterial toxins (chapter 9.1), in particular Shiga toxin and Shiga-like toxins (SLTs) (chapter 9.1.2). Small molecule inhibitors of these toxins are summarized afterwards in chapter 9.1.3, notably the Retro-2 compound. Since these toxins rely on intracellular trafficking to reach their molecular targets, a general overview of endocytosis and endosomal trafficking is provided (chapter 9.2). Next, the retrograde route entry is presented (chapter 9.2.5), with focus on clathrin, the retromer and GPP130, a protein that constantly cycles between Golgi, plasma membrane, and endosomes. SNARE proteins, particularly syntaxin-5 and syntaxin-16, are then introduced (chapter 9.2.6). After a brief section of the micro RNA family 199 (chapter 9.3), the introduction finishes with the description of some salient techniques that were used in my work, such as - bio-orthogonal Click-Chemistry, anterograde trafficking synchronization with the retention using selective hooks (RUSH) assay, and the antibody-based proximity ligation assay (chapter 10.6.1, 0, 10.11.1).Herein, my submitted publication opens the results part (chapter 11.1), in which I present the utility of biorthogonal click chemistry for the search of the cellular targets of Retro-2, a small molecule inhibitor that was previously shown to protect cells and animals against Shiga toxin and ricin. I describe that Sec16A is a likely cellular target candidate, and illustrate using the RUSH approach how interfering with Sec16A functions leads to the partial relocalization of syntaxin-5 to the endoplasmic reticulum (ER) by slowing-down its anterograde transport. The second part of the paper describes how syntaxin-5 relocalization causes the inhibition of Shiga toxin trafficking from endosomes to the TGN. I present a novel interaction between syntaxin-5 and the Golgi protein GPP130, which both have been already described in relation to Shiga toxin trafficking. My work connects both trafficking factors in retrograde trafficking at the endosomes-TGN interface. Strikingly, I demonstrate that this interaction is most probably based on a non-SNARE function of syntaxin-5.In collaboration with Juan Francisco Aranda and Carlos Fernandez in the US, we put micro RNAs into an endogenous regulation context of Shiga toxin retrograde trafficking (chapter 11.2). An extended discussion will be given in chapter 12.Last, a general outlook of ongoing projects is given in the perspectives section (chapter 13), in which further collaborations are highlighted.Keywords: Retrograde transport, Shiga toxin, Shiga-like toxin (SLT), STxB, syntaxin-5, Sec16A, GPP130, Retro-2, Retro-2.1, azide-functionalized Retro-2, copper-free click chemistry, small molecule target identification, mass spectrometry, non-SNARE function, anterograde trafficking inhibition, miRNA, miR199, retromer, VPS26
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Patogenetické mechanismy podmiňující vznik a rozvoj hemolyticko-uremického syndromu u dětí / Pathogenetic mechanisms determining the origin and development of a hemolytic-uremic syndrome in childrenKarnišová, Lucia January 2021 (has links)
Hemolytic uremic syndrome (HUS) induced by Shiga toxin-producing E. coli (STEC) is the most common causes of acute kidney injury in children. The therapy of the disease is symptomatic and the main factors leading to the development of severe course of a STEC-HUS are still unknown. In our study, we dealt with factors leading to development of a severe course of STEC-HUS in pediatric patients on both the host and pathogen side. Using retrospective analysis of the courses in children in the Czech Republic, we found that the most common cause of STEC-HUS was serotype O26 and HUS most often affected children under 3 years of age. 63,8 % required dialysis and mortality was 8.62 %. On the host side we focused on the relationship between the activation of the alternative complement pathway and the severity of the course of HUS. We found a significant difference in the level of the C3 part of complement in patients who required dialysis and patients for whom dialysis was not necessary. We also a cut-off value for the C3 part of complement and its reduction below 0.825 g / l was associated with the need for dialysis treatment and a higher incidence of extrarenal complications. Based not only on our results, it can be assumed that the therapeutic effect of complement could affect the severity of the disease....
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Targeting strategies using B-subunit of Shiga toxin : innovative drug-delivery systems / Stratégies de vectorisation par la sous-unité B de la toxine de Shiga : systèmes de libération d’agents cytotoxiques innovantsBatisse, Cornélie 28 January 2015 (has links)
Les stratégies thérapeutiques mises en place contre le cancer ont de nos jours besoin de nouveaux médicaments, à la fois plus actifs que ceux déjà existants et induisant moins d’effets secondaires. Ces nouvelles stratégies visent à cibler spécifiquement les cellules cancéreuses. Parmi ces stratégies, ces travaux de thèse concernent la vectorisation active, à l’aide d’un vecteur protéique dérivé de la toxine de Shiga, STxB. STxB reconnait spécifiquement son récepteur biologique Gb3, surexprimé à la surface des cellules cancéreuses humaines. Ce projet de recherche porte sur la conception et la synthèse de conjugués, combinant STxB et un agent cytotoxique. Le linker chimique, qui relie ces deux espèces, a été soigneusement conçu pour respecter les deux critères suivants : être suffisamment stable et néanmoins pouvoir être clivé pour libérer l’agent cytotoxique une fois les cellules cancéreuses atteintes. Un premier linker a été construit autour du motif mercaptoethanol, lié au vecteur STxB par une liaison disulfure. La libération de l’agent cytotoxique peut donc être initiée par un réducteur biologique comme le glutathion, puis par une étape d’auto-immolation. Ce linker a été appliqué à deux composés cytotoxiques très puissants, dérivés de l’auristatine, et a conduit à des résultats prometteurs in vitro. La labilité de la liaison ester à pH acide a également été mise à profit dans l’élaboration de deux linkers, conçus autour de motifs glutamate et thréoninate. L’utilisation d’un agent cytotoxique modérément puissant a été l’occasion de développer une stratégie de multivalence, consistant à augmenter la charge d’agents cytotoxiques sur STxB. Une autre option a été de considérer les nano-batônnets d’or comme une plate-forme nanométrique multimodale, capable de lier plusieurs milliers d’agents cytotoxiques et STxB. Enfin l’incorporation d’une séquence peptidique, connue pour être substrat d’une protéase, a donné lieu à une troisième étude, reposant sur un linker clivable plus sélectivement. Plusieurs linkers ont été étudiées, selon qu’ils libèrent l’agent cytotoxique sous sa forme native ou non. / We need new therapeutic strategies to treat cancerous patients by the discovery of new drugs that would be more active than those existing and especially assigning fewer side effects. These new therapies aim to specifically target cancer cells. Among the strategies for cancer targeting, we investigated drug-targeted strategies using a proteic carrier, STxB, derived from Shiga toxin. This protein recognizes specifically its biological receptor Gb3, which is over-expressed on human cancer cells. This work consisted in the design and synthesis of conjugates combining STxB and a cytotoxic drug. The chemical linker binding these two moieties was carefully designed in order to fit requirements of both stability and ability to trigger a drug-delivery. A first linker was designed around a mercaptoethanol core, able to be conjugated to STxB by a disulfide bond. This constitutes a drug-delivery trigger, activated by a biological reducing agent such as glutathion, and followed by a self-immolative step. Two highly potent conjugates of auristatin derivatives were obtained and showed promising results in vitro. The ester bonds lability in acidic pH was exploited for the design of two amino acid based linker. With the aim of increasing the ratio of drug on STxB, we investigated several multivalent linkers. Another option was to consider gold nanorods as a nanometric platform, able to carry thousands of drugs and STxB. The incorporation of a protease substrate to produce an enzyme-cleavable linker was investigated. Several spacers, which induced release of the drug under native form or under prodrug form, were designed and tested.
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Avaliação da exposição do consumidor à Listeria monocytogenes, Salmonella spp., Campylobacter spp. e Escherichia coli produtora de toxina de Shiga em produtos cárneos refrigerados comercializados no município de São Paulo / Assessment of consumer exposure to Listeria monocytogenes, Salmonella spp., Campylobacter spp. and Shiga toxin-producing Escherichia coli in refrigerated meat products at retail in São Paulo municipalityCosta, Christiane Asturiano Ristori 30 March 2010 (has links)
As Enfermidades Transmitidas por Alimentos representam um crescente e relevante problema de saúde pública. Além do prejuízo social, a contaminação de alimentos com microrganismos patogênicos gera um enorme prejuízo econômico. Técnicas de Análise de Risco permitem mensurar de forma mais adequada o impacto dos microrganismos contaminantes de alimentos na saúde da população. Uma Análise de Riscos, associada a uma combinação patógeno-alimento, envolve três passos: avaliação do risco, gestão do risco e comunicação do risco. Uma das etapas da avaliação do risco é a avaliação da exposição, baseada em dados sobre freqüência e nível de contaminação dos alimentos pelo patógeno avaliado no alimento em questão, o nível atingido pelo patógeno no momento do consumo e os padrões de consumo. Os produtos cárneos são os principais alimentos responsáveis pela veiculação de patógenos ao homem e os microrganismos de maior relevância nestes produtos são Listeria monocytogenes, Salmonella spp., Campylobacter spp. e Escherichia coli produtora de toxina de Shiga. O objetivo do presente estudo foi levantar informações qualitativas e quantitativas desses quatro patógenos em produtos cárneos (salsicha bovina, lingüiça suína, carne bovina moída e coxa de frango) comercializados no município de São Paulo, de forma a contribuir com dados para futuras avaliações de risco em relação a estes microrganismos nestes produtos. Das 552 amostras de produtos cárneos analisadas, L. monocytogenes foi o patógeno isolado com maior freqüência, sendo detectado em 48,7% das amostras, seguido por Campylobacter spp. em 6,0% e Salmonella spp. em 5,8%. E. coli produtora de toxina de Shiga não foi detectada em nenhuma das amostras estudadas. Listeria monocytogenes foi detectada em todos os tipos de produtos cárneos estudados, com freqüências mais elevadas nas amostras de carne bovina moída (59,4%), seguido de coxa de frango (58,0%), lingüiça suína (39,8%) e salsicha bovina (37,7%). Na maioria das amostras (94,4%), as contagens de L. monocytogenes foram inferiores a 102 UFC/g. As cepas de L. monocytogenes apresentaram ampla distribuição, sendo detectados os quatro grupos de sorotipos: 28,7% pertenceram ao Grupo 1 (sorotipos 1/2a e 3a), 21,0% ao Grupo 2 (sorotipos 1/2c e 3c), 17,0% ao Grupo 3 (sorotipos 1/2b, 3b e 7) e 13,8% ao Grupo 4 (sorotipos 4b, 4d e 4e). Salmonella spp. foi detectada em 32 amostras, sendo 20 (14,5%) de lingüiça e 12 (10,6%) de coxa de frango. As contagens foram baixas, variando de 3,0 a 9,3x10 NMP/g e os sorovares mais freqüentemente isolados foram S. Typhimurium (28,1%), S. Enteritidis (12,5%), S. Derby (12,5%) e S. I 4,[5],12:i:- (12,5%). Campylobacter spp. foi detectado em 33 amostras (6,0%), sendo 27 de coxa de frango (19,6%) e seis amostras de carne moída (4,3%). A presença de L. monocytogenes, Salmonella spp. e Campylobacter spp. nos produtos cárneos analisados representa um risco à saúde da população. O consumo destes produtos quando submetidos à cocção inadequada e/ou a contaminação cruzada com outros alimentos pode levar a ocorrência de Enfermidades Transmitidas por Alimentos. / Foodborne Diseases represent an increasingly important public health problem. Besides the social losses, contamination of food with pathogenic microorganisms generates an enormous economic damage. A more accurate measurement of the impact of microorganisms in food health can be achieved using Risk Analysis techniques. A risk analysis is composed by three elements: risk assessment, risk management and risk communication. One of the four steps of a risk assessment is the exposure assessment, based on data on frequency and level of contamination of a food by the pathogen under evaluation, levels of the pathogen in the food at the time of consumption and consumption patterns. Meat products are the main vehicles of pathogens to humans, where Listeria monocytogenes, Salmonella spp., Campylobacter spp. and Shiga toxin-producing Escherichia coli are the most relevant pathogens. The aim of this study was to obtain qualitative and quantitative information on these four pathogens in four types of meat products (beef sausage, pork sausage, ground beef and chicken leg) marketed in the city of Sao Paulo in order to contribute with data for future risk assessments for these microorganisms in these products. L. monocytogenes is the most frequent pathogen in the 552 samples of meat products analyzed, being detected in 48.7% of the samples, followed by Campylobacter spp. 6.0% and Salmonella spp. 5.8%. Shiga toxin-producing E. coli was not detected in any sample. L. monocytogenes was detected in all types of meat products, with highest frequency in ground beef (59.4%), followed by chicken leg (58.0%), pork sausage (39.8%) and beef sausage (37.7%). In most samples (94.4%), the counts of L. monocytogenes were below 102 CFU/g. L. monocytogenes strains were widely distributed in the four groups of serotypes: 28.7% belonged to Group 1 (serotypes 1/2a and 3a), 21% to Group 2 (serotypes 1/2c and 3c), 17% to Group 3 (serotypes 1/2b, 3b and 7) and 13.8% to Group 4 (serotypes 4b, 4d and 4e). Salmonella spp. was detected in 32 samples, being 20 (14.5%) of pork sausage and 12 (10.6%) of chicken leg. The counts were low, ranging from 3.0 to 9.3 x 10 MPN/g and the most frequent serovars were S. Typhimurium (28.1%), S. Enteritidis (12.5%), S. Derby (12.5%) and S. I 4, [5], 12: i: - (12.5%). Campylobacter spp. was detected in 33 samples (6.0%), being 27 of chicken leg (19.6%) and six samples of ground beef (4.3%). The presence of L. monocytogenes, Salmonella spp. and Campylobacter spp. in the tested meat products represent a risk to health. The consumption of inadequately cooked products and/or subjected to cross-contamination with other foods may lead to occurrence of foodborne diseases.
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