Thesis (Ph. D.)--University of Missouri-Columbia, 2002. / Typescript. Vita. Includes bibliographical references (leaves 137-143). Also available on the Internet.

Thesis (Ph. D.)--University of Missouri-Columbia, 2002. / Typescript. Vita. Includes bibliographical references (leaves 137-143). Also available on the Internet.

Myeloid-derived suppressor cells (MDSC) are a major component of the immune suppressive network that develops during cancer. MDSC down-regulate immune surveillance and antitumor immunity and facilitate tumor growth. The ability of MDSC to suppress T cell responses has been documented; however the mechanisms regulating this suppression remain to be understood. This work proposes a biological dichotomy of MDSC regulated by the tumor microenvironment. In peripheral lymphoid organs MDSC cause T-cell non-responsiveness that is antigen-specific. These MDSC have increased expression of NOX2, enabling them to produce large amounts of reactive oxygen species. Since the transcription factor STAT3 is substantially activated in MDSC, its potential role in upregulation of NOX2 expression was investigated. Over-expression of a constitutively active form of STAT3 increases expression of NOX2 subunits, whereas attenuation of STAT3 activity leads to decreased expression of NOX2. The significance of NOX2 in ROS generation is demonstrated in mice devoid of NOX2 function; NOX2- deficient MDSC are unable to inhibit antigen-induced activation of T cells. In contrast, MDSC within the tumor microenvironment have a diminished potential to generate ROS but acquire expression of arginase and inducible nitric oxide synthase, enzymes plicated in T cell non-responsiveness. Upregulation of these enzymes results in MDSC ability to inhibit lymphocyte response in absence of antigen presentation. The tumor microenvironment also promotes the differentiation of MDSC to tumor associated macrophages. Hypoxia is an exclusive feature to the tumor microenvironment and we investigated its involvement in the properties of MDSC at the tumor site. Exposure of spleen MDSC to hypoxia converts MDSC to non-specific suppressors and induces a preferential differentiation to macrophages. Stabilization of HIF-1!, a transcription factor activated by hypoxia, induces similar changes in MDCS as hypoxic exposure. Finally, ablation of HIF-1! prevents MDSC from acquiring factors that enable the suppression of T cells in absence of antigen. These findings help to expand our understanding of the biology of MDSC and suggest a regulatory pathway of myeloid cell function exclusive to the tumor microenvironment. They may also open new opportunities for therapeutic regulation as we now should take into consideration how systemic location affects the function of MDSC.

In this dissertation, multicarrier schemes are reviewed within the framework of Gabor Systems. Their fundamental elements; what to transmit, i.e., symbols, how to transmit, i.e., filters or pulse shape, and where/when to transmit, i.e., lattices are investigated extensively. The relations between different types of multicarrier schemes are discussed. Within the framework of Gabor systems, a new windowing approach, edge windowing, is developed to address the out-of-band (OOB) radiation problem of orthogonal frequency division multiplexing (OFDM) based multicarrier schemes. To the best of our knowledge, for the first time, the diversity on the range of the users is exploited to suppress the sidelobes of OFDM. In addition to that, the concept of using different filters in OFDM structure is proposed. Besides the improvement on the OOB radiation performance of OFDM via edge windowing, conventional lattice structure of OFDM frame is enhanced considering the diversity in the network. The lattice structure of an OFDM frame is designed based on the statistical characteristics of the range of the users and the mobility. The concept of channel-aware frame structure is developed, which allows more efficient and reliable transmission. In addition to the aforementioned improvements on OFDM, interference issues in uncoordinated networks are addressed in this dissertation considering different multicarrier schemes. It is stressed that the interference from other links in the network sharing the same spectrum might degrade the link performance between the devices in an uncoordinated network, significantly. Considering the degradation due to other-user interference, the concept of partially overlapping tones (POT) is proposed. With the concept of partially overlapping tones, the interference energy observed at the victim receiver is mitigated via an intentional frequency offset between the links. The usefulness of intentional frequency offset to combat with the asynchronous nature of other-user interference without any timing constraint between interfering signals is emphasized. To the best of our knowledge, for the first time, the efficacy of non-orthogonal schemes are shown along with POT to address the other-user interference, which relies on the fact that self-interference problem is easier than other-user interference problem in an uncoordinated network. In the last part of this dissertation, required number of equalizer taps for multicarrier schemes is investigated to address the potential self-interference problems (e.g. due to the non-orthogonal multicarrier schemes with the concept of POT). Composite impact of transmit pulse shape, communication medium, and receive filter on the characteristics of the interference among the symbols in time and frequency is analyzed. It is emphasized that while taking less number of taps into account for the channel estimation causes lack of description of the composite effect, using more number of taps folds the noise into the estimated channel. The number of interfering symbols and their locations are obtained in both time and frequency for a given multicarrier scheme and signal-to-noise ratio. It is shown that correct number of taps yields not only improvement on BER performance but also less complex equalizer structures in practice.

This project aims to improve research in gamma-ray spectroscopy by using advanced detector systems. These systems are designed to reduce interference inherent in gamma-ray spectroscopy by rejecting Compton scattering events from high-energy gamma-rays, as well as look at cascading decays of gamma-rays through gamma-gamma coincidence counting. By combining these methods, one is able to lower detection limits for many elements than would otherwise be possible. This work also takes advantage of neutron activation analysis, which allows stable elements to be analyzed by activating them with neutrons, causing them to become unstable and decay with radioactive signatures. By analyzing these signatures, one is able to detect trace levels of elements with relatively small samples sizes (< 1g) and in a nondestructive manner. / text

In this research, in order to realize such nanolaminated magnetic cores for high frequency and high power conversion, the following key tasks have been accomplished: 1) electrodeposition of metallic alloy materials such as NiFe, CoNiFe, and anisotropic CoNiFe; 2) development of new fabrication technologies to realize nanolaminated cores based on metallic alloy electrodeposition; 3) reliable characterization of the structural, magnetic, and electrical properties of the nanolaminated metallic alloy cores; 4) development of microfabricated inductor windings to integrate the nanolaminated cores; 5) demonstration of high-frequency and high-flux ultracompact DC-DC power conversion using inductors integrated with nanolaminated metallic alloy cores. By achieving these tasks, nanolaminated cores comprising tens to hundreds of layers of metallic alloy films (Ni80Fe20 and Co44Ni37Fe19) has been developed. The fabricated nanolaminated core consists of sufficiently thin nanolaminations (100 – 1000 nm) that can suppress eddy currents in the MHz range, while simultaneously achieving the overall magnetic thickness (35 – 2000 µm) such that substantial power can be handled. The nanolaminated metallic alloy cores were further integrated into microfabricated inductors using CMOS-compatible fabrication processes. Finally, an ultracompact DC-DC buck converter with the nanolaminated metallic alloy cores has been developed on PCB having footprint of 14 × 7.1 mm2. The input voltage of the converter varied from 30 to 70 V and the output voltage was fixed at 20 V. The converter operated with output power of approximately 11 W and the switching frequencies of 0.7 – 1.4 MHz, demonstrating conversion efficiency of 94.2% at 30 V input and 80.8% at 60 V input.

During every cell cycle and upon exogenous stress, tumor suppression programs are engaged to ensure genomic stability. In response to replicative aging and oncogenic stimuli, the p53 and Rb pathways are activated to prevent the proliferation of damaged cells. Several lines of evidence suggest that escape from senescence is a crucial early step in oncogenic progression. A major challenge in the cancer field is to combine genomic information regarding cancer-associated genetic changes with high-throughput functional studies, in order to confirm genetic requirements and pinpoint biological roles of these perturbed genes in oncogenesis. Furthermore, a complete genetic understanding of replicative senescence, and how it might be bypassed, is lacking. We describe here two genome scale loss-of-function genetic screens that interrogate these tumor suppressor programs. We utilized a unique sensitization approach to isolate senescence pathways and unmask compensatory mechanisms that may have been difficult to identify in previous studies. These genetic screens have generated comprehensive and validated datasets of putative senescence and p53 pathway genes. We present this dataset as a high-quality resource for further investigation into these biological pathways. We have uncovered several genes in distinct biological pathways which have not been demonstrated to have a functional role in senescence, and which may be putative tumor suppressors. We have identified BRD7 and BAF180, two SWI/SNF components, as critical regulators of p53. BRD7 and BAF180 are required for p53 activity and p21 expression during replicative and oncogene-induced senescence, and evidence suggests that they are inactivated in human cancer. In addition, we have uncovered a role for the deubiquitinating enzyme USP28 in the regulation of p53 accumulation during senescence, such that loss of USP28 results in bypass of the senescence program. We have also investigated several other novel senescence genes including SEMA6A, SEMA3b, and TMEM154. We have found that the expression of these genes is highly regulated during senescence by distinct means, including both p53-dependent and p53-independent mechanisms. These results demonstrate the efficacy of our sensitized screening approach, and also highlight the emerging view that the senescence program requires the combined action of multiple biological pathways for its execution.

Emotion regulation involves changing the emotions that one experiences and expresses. Many studies have shown that culture influences emotion regulation and that regulating emotions can have positive or negative effects on relationship quality (English & John, 2013; Impett et al., 2011; Kalokerinos, Greenaway, & Denson, 2014; Regan, Lakhanpal, Anguiano, 2012; Su, Wei, & Tsai, 2014; Yelsma & Athappilly, 1988). Although research has studied culture, emotion regulation, and relationship quality separately, essentially no studies have combined the three factors to see how they are related. Specifically, there is very little information on whether emotion regulation plays a similar role in relationship quality across different cultures. The present study looks at daily associations between emotion regulation strategies (reappraisal, suppression, avoidance, and taking action) and emotions felt due to the partner, moderated by marriage type (American, Indian-arranged, Indian-love). I compare 128 heterosexual couples from the United States and India (in love and arranged marriages). These couples completed daily diaries once a day for seven days and reported the types of emotion regulation they used, as well as their positive or negative emotions due to their partner. I found that for Indian love and arranged couples, taking action was associated with increased positive partner induced emotions and reduced negative emotions. In contrast, taking action for U.S. couples had the opposite effect and was associated with increased negative emotions due to the partner, with no effect on positive emotions. In the U.S. couples, I also found that suppression and avoidance were associated with decreased positive partner induced emotions, but there was no effect for the Indian couples. With reappraisal, I found that high levels were associated with decreased negative partner induced emotions for all couples. Overall, results suggest that effective regulation of negative partner induced emotions can protect relationship quality, but that what constitutes effective regulation depends on cultural context.

Στην παρούσα διπλωματική αναπτύχθηκε μια μέθοδος ψηφιακής επεξεργασίας σημάτων για ακουστικά σήματα συμβατή με πραγματικού χρόνου επεξεργασία. Αρχικά έγινε περίληψη των λειτουργιών των επεξεργαστών ειδικού σκοπου. Έγινε μελέτη της ανάλυσης στο πεδίο της συχνότητας καθώς και της συνάρτησης συνεκτικότητας. Για τους σκοπούς της διπλωματικής υλοποιήθηκε αλγόριθμος αφαίρεσης θορύβου από σήματα ομιλίας που αξιοποιεί την συνάρτηση συνεκτικότητας και χρησιμοποιεί είσοδο από δύο μικρόφωνα. Ο αλγόριθμος αυτός υλοποιήθηκε και δοκιμάστικε σε μη-πραγματικό χρόνο σε μαθηματικό λογισμικό , καθώς και σε πραγματικό χρόνο σε επεξεργαστή ειδικού σκοπού. / In this thesis, a method of digital signal processing for acoustic signals was developed, compatible with real-time processing. At first, a review of the operations that special purpose digital signal processors feature. We also studied the frequency domain analysis and the coherence function in depth. For the purposes of this thesis an algorithm of noise reduction from speech signals was implemented, that exploits the coherence function and takes two microphone signals as inputs. The algorithm was implemented offline in a mathematical software, as well as real time in a special purpose digital signal processor.

Interactions among site conditions, disturbance events, and climate determine the patterns of forest species recruitment and mortality across landscapes. Forests of the American Southwest have undergone significant changes over a century of altered disturbance regimes, human land uses, and changing environmental conditions. This study reconstructs the interactions between fire, spruce beetle outbreaks, climate, and anthropogenic factors and their influence on the species composition, spatial extent, and structure of four upper elevation forest types. We found that fire-climate associations changed following fire exclusion and recent high-severity fires occurred during less severe conditions than in several larger, lower severity fires in the historical record. Contemporary fires are burning with higher severity than similarly-sized historical fires, suggesting a shift toward higher-severity fire as a result of changes to forest structure and fuels over much of the upper elevation forest. In high elevation forests, the area occupied by Engelmann spruce and corkbark fir doubled in size over the four decades following fire exclusion. The increase in spruce beetle outbreak size and severity in the 20th century appears to be linked to significant expansion of host extent, accelerated growth of spruce in mixed-conifer forest, and incidence of anomalously warm summer temperatures followed by up to a decade of low precipitation. Trends toward warming, drying conditions are expected to increase the risk of future high-severity outbreaks, especially in locations of recent spruce population expansion. Forest conversion from disturbance-adapted to competition-adapted species following fire exclusion was a function of site productivity. Species assemblages in the lowest and highest productivity sites were the most stable over the century following fire exclusion. Frequent low severity fires maintained the stocking of forests in moderate productivity sites below their biological potential, conferring a degree of resistance to drought, insect outbreaks, and high-severity fire prior to fire exclusion. Current forests located on moderate productivity sites are now the most vulnerable to drought and future disturbance. Aggressive action to restore historical species composition, stocking and fire component of these forests may return resilience to this system in the face of projected changes to fire and climate dynamics.