Collaborative tagging is a simple and effective method for organizing and sharing web resources using human created metadata. It has arisen out of the need for an efficient method of personal organization, as the number of digital resources in everyday lives increases. While tagging has become a proven organization scheme through its popularity and widespread use on the Web, little is known about its implications and how it may effectively be applied in different situations. This is due to the fact that tagging has evolved through several iterations of use on social software websites, rather than through a scientific or an engineering design process. The research presented in this thesis, through investigations in the domain of e-learning, seeks to understand more about the scientific nature of collaborative tagging through a number of human subject studies. While broad in scope, touching on issues in human computer interaction, knowledge representation, Web system architecture, e-learning, metadata, and information visualization, this thesis focuses on how collaborative tagging can supplement the growing metadata requirements of e-learning. I conclude by looking at how the findings may be used in future research, through using information based in the emergent social networks of social software, to automatically adapt to the needs of individual users.

In recent years, passive radio frequency (RF) tags that communicate using modulated backscatter radiation have shown great potential for use in inventory management, parcel and postal tracking, for use as remote sensors, and in a host of other Radio Frequency Identification (RFID) applications. However, for the widespread use of these tags to become reality, much basic research is needed to reduce the cost, increase the range, and increase the reliability of the RF tag. This research seeks to enhance the performance of passive RF tags by developing a series of tests, or radio assay, to measure the following: the performance of RF tag antennas as a function of antenna material and manufacturing technique and the antenna performance when attached to various materials. The radio assay experiments are designed for RF tag antennas that operate in the far field of the tag reader and communicate using modulated backscatter radiation at 915 MHz. Three flexible, folded dipoles, printed on plastic substrates, were measured in the radio assay experiments. The results of the experiments include the following: the antenna gain penalty (relative to a baseline antenna) for each antenna material and manufacturing technique, the antenna gain penalty (relative to the free space antenna gain) due to material losses when the tag antenna is attached to an object, and the benefits (in terms of antenna gain) of tuning each tag antenna to the material to which it is attached. The results are presented in a form to aid RF engineers in the design of RF tag system link budgets.

This study was to determine the effects of several selected environmental factors (temperature, salinity, light intensity, and acetate) on the nitrogen starvation induced lipid accumulation in Chlamydomonas reinhardtii CC 400 by the Nile Red staining of lipid in the cells. Nitrogen starvation induced lipid accumulation, the extent of lipid accumulation increased as nitrogen concentrations in the medium decreased. For the 9.4 mM NH4Cl of HS medium as 100% N, the absence of NH4Cl from the medium will show the maximum induction in the lipid accumulation. This was also observed in the treatment of algal cells in mid-log phase by the absence of NH4Cl in the medium. A decrease in temperature down to 15oC depressed the nitrogen starvation induction in lipid accumulation for the algal cells from the mid-log phase, while the elevation in the light intensity up to 300 £gmol photons • m-2 • s-1 also showed an inhibitory effect. However, the transfer to darkness for the nitrogen starvation also inhibited the lipid increase. The addition of 100 mM NaCl enhanced the nitrogen starvation induced lipid accumulation but the NaCl level up to 200 mM inhibited the increment. The nitrogen starvation induction of lipid increase was partly inhibited due to the absence of acetate, whereas the increase in acetate concentrations in the medium did not have effect on lipid accumulation as compared to normal acetate addition in the medium. Overall, the results of the present study show that light and acetate are essential factors for the maximum lipid accumulation in C. reinhardtii by nitrogen starvation.

From the sexual preselection point of view, understanding sex determination/differentiation mechanisms in the bird is critical in both evolutionary and industrial applications. The chicken embryo provides an unique vertebrate model in the field of development biology. Morphological sex development in the chick gonad starts at 6.5 embryonic day (E6.5), however, genetic sex determination and development should occur earlier. In order to comprehend genes and their underlying mechanisms being involved in sex-determination/development during early embryogenesis, we not only made a male-subtract-female and a female-subtract-male cDNA library as early as embryonic day 3 (E3; Hamburger and Hamilton Stage 20), but also examined early transcripts related to male development in chicken embryo and their expression profiles in this study. A total of 89 and 127 candidates of male-development transcripts represented respectively for 83 known and 119 unknown non-redundant sequences, which were characterized in an E3 male- subtract-female complementary DNA library. In this study, thirty-five selected transcripts being validated by quantitative reverse transcription-polymerase chain reaction that the expression levels of 25 transcripts were higher in male E3 whole embryos than in females (P < 0.05). Notably, twelve of these transcripts mapped to the Z chromosome. At 72 weeks of age, twenty transcripts were expressed at higher levels in testes than in ovaries. Meanwhile, four transcripts were expressed at higher levels in brains of male than in brains of female chickens (P < 0.05). By using of methods of whole mount and frozen cross-section in situ hybridization, the expressions of riboflavin kinase (RFK), WD repeat domain 36 (WDR36) and EY505808 transcripts on E7 chicken male gonads were corroborated to be better than female gonads. This result was confirmed by using of western blotting analysis which also showed the expressions were specifically on gonads than other tissues. Treatment with an aromatase inhibitor formestane at E4 depicted the effect of the expression levels at E7 of the coatomer protein complex (subunit beta 1), solute carrier family 35 member F1, LOC427316 and EY505812 transcripts across both sexes (P < 0.05), which was similar to the observed gene expressions for both doublesex and mab-3 related transcription factor 1 gene. Additionally, the interaction effects of sex with formestane treatment were observed in 15 candidate male development transcripts (P < 0.05). This study demonstrated a panel of potentially candidate male development transcripts being identified during early chicken embryogenesis; some might be regulated by sex hormones.

A measurement method for characterizing RFID chip has been proposed that can measure the approximate Read/Write threshold power and impedance of RFID strap with minimum operating procedures; furthermore, the complicated RF facilities are not required. Obtaining the specifications of RFID strap allows designers to estimate maximum read range of designed RFID tag in advance. Therefore, the implemented cost and design cycle times can be reduced substantially. For the verification of the final match condition of assembled RFID tag, a direct measurement technique has been developed, which not only can verify the final impedance match condition of the assembled RFID tags, but can also be used to identify the resistance and reactance mismatch condition between the RFID chip and antenna. The measurement data obtained from the verification method can also be used to estimate the assembly error introduced by different mounting methods. The use of the corrected circuit model of the RFID chip impedance, which includes the assembly error, helps improve the accuracy of the RFID tag design. In the RFID metal tag design, a series of low profile and miniature RFID tags, which is directly attached on metallic objects, has been developed. A series of low cost and easily produced RFID tag antenna structures also has been realized for RFID application on hanging metallic tag, which makes the RFID solution well suited for metallic tag of labeling system that requires integration of RFID technology. The attached RFID metal tag and the metallic RFID hanging tag cover most of the RFID application on steel products in the steel industry.

Expressed sequence tags (ESTs) are derived from the developing tilapia brain was established in our lab. There are 9 transcripts were identified as thermosensitive/Neural development-related gene. The effects of different temperatures on the ontogenetic expression of these thermosensitive/Neural development-related gene during the critical period of brain sexual differentiation were investigated in the present study. The ontogenetic expression of inhibitor of DNA binding/differentiation protein 2 (Id2), thermosensitive/Neural development-related gene, were enhanced by both lower (20¢J) and higher (32¢J) temperatures before 10 days post-hatching. In this study, bioinformatics were searched for Id2, which is a gene with 738 bp of patial cDNA sequence, open reading frame (ORF) is 411bp, and deduced 137 amino acids of protein sequence. The protein of Id2 was expressed in a prokaryotic system, BL21 (Escherichia coli) and purified with Ni-NTA affinity chromatography. Also, the ORF of Id2 was cloned into pEGFP vector, and plasmid (pEGFP-Id2) was transfected into the eukaryotic system, mouse neuroblastoma cell (Neuro-2a cell). The distribution of Id2 expressed in the Neuro-2a cell was identified by fluorescence microscopy.

Dieser Vortrag entspricht in seinem Inhalt dem ersten Kapitel des ZIN - Zertifikat Internet Nutzung der TU Chemnitz.

Depuis quelques années le nouveau paradigme d'internet des objets, qui se traduit par la connexion des objets de la vie quotidienne à internet grâce à des dispositifs électroniques intégrés, est devenu plus qu'un simple concept et a commencé à entrer dans la réalité. Il en est ainsi des systèmes d'identification par radiofréquence (RFID), qui font partie des technologies disponibles. Lorsque le coût et la taille des tags deviennent des exigences prépondérantes, l'adoption de tags (semi-)passifs fondés sur la modulation d'un signal rétrodiffusé représente une solution attractive. En outre, l'utilisation de techniques ULB apporte des avantages propres à favoriser le remplacement d'une partie des systèmes RFID actuels qui souffrent de certains défauts tels qu'une trop forte consommation ou une faible robustesse en présence d'interférence. La thèse de doctorat se place dans ce contexte: elle a pour objectif l'étude des systèmes ULB RFID semi-passifs et se situe dans le cadre du projet européen SELECT, qui vise à développer et à évaluer un tel système. La prise de conscience qu'il était fondamental de comprendre les mécanismes électromagnétiques intrinsèques à la communication lecteur-tag, préalablement à la conception du système, s'est traduite par la mise en oeuvre d'une collaboration franco-italienne dans le cadre d'une cotutelle de thèse. C'est donc au départ autour d'une analyse fine et d'une modélisation des aspects antennaires et du canal radio entre le lecteur et le tag que la 1re partie du travail s'est construite. La 2nde partie a exploité ces travaux pour l'élaboration des schémas de modulation et de codage du lecteur et du tag pour les systèmes semi-passifs.

Réovirus, connu sous le nom REOLYSIN®, est présentement à l'étude à titre d'agent oncolytique. Or, la spécificité du virus pour les cellules cancéreuses pourrait être optimisée par une modification au niveau de la protéine d'attachement σ1. La présente étude vise à démontrer qu'une telle amélioration est possible par l'utilisation de la méthode nouvellement décrite de génétique inverse. Par cette technique, il est possible d'ajouter un polypeptide d'une longueur de quarante acides aminés à l'extrémité C-terminale de σ1. Il est aussi possible d'engendrer des virus mutés en leur site d'activité mucinolytique. Les virus nouvellement créés démontrent une efficacité de réplication diminuée, mais demeurent infectieux. Contrairement aux méthodes traditionnellement utilisées avec réovirus, la méthode de génétique inverse permet de conserver les mutations engendrées, par substitution ou addition, au cours des cycles de réplication. Une telle étude démontre qu'il serait possible de modifier le tropisme de réovirus. / Reovirus, also known under the tradename REOLYSIN®, is currently under clinical trial as an oncolytic agent. The specificity of the virus for transformed (cancerous) cells could be improved by modifications of the σ1 attachment protein. This study presents two ways to achieve this improvement by using a newly developed method of reverse genetics for double-stranded RNA viruses. As a proof of concept, we engineered the addition of an exogenous polypeptide of forty amino-acids to the C-terminal end of σ1. Subsequently, we created viruses with compromised mucinolytic activities by introducing amino-acids substitutions in the appropriate genetic sites. A decreased replicative efficacy was observed in all experimentally generated viruses, despite maintaining their infectious capacity. Reverse genetics allows the generation of viruses that retain their mutations throughout successive replicative cycles, which was impossible with traditionnal techniques. This project demonstrates the feasibility of tropism modification by using the reverse genetics method.

Cette recherche propose d'examiner les marqueurs de l'anglais à la lumière du concept de polyphonie, abondamment utilisé en linguistique du français. Cette étude du cas de l'interro-négative en anglais questionne le caractère opératoire de ce concept : renseigne-t-il aussi le fonctionnement de la langue anglaise ?Pour répondre à cette question, nous proposons tout d'abord un retour sur les bases théoriques qui ont inspiré notre travail, pour les mettre ensuite à l'épreuve de nos occurrences d'interro-négatives extraites de notre corpus de nouvelles de Raymond Carver. Nous analysons ainsi, dans un premier temps, les interro-négatives sans pronom interrogatif, introduites par isn't, don't et didn't. Dans un deuxième temps, nous portons notre attention sur la question ouverte introduite par le pronom interrogatif why. Enfin, les question-tags sont abordées : elles permettent d'asseoir notre thèse quant à la place importante qu'occupe l'interlocuteur dans la construction de message. A cet égard, nous réhabilitons son rôle dans le processus de construction de message en lui attribuant celui de co-constructeur