The tags and descriptions entered by video owners in video sharing sites are typically inadequate for retrieval purposes, yet the majority of video search still uses this text. This problem is escalating due to the ease with which users can self-publish videos, generating masses that are poorly labelled and poorly described. This thesis investigates how users tag videos and whether video tagging games can solve this problem by generating useful sets of tags. A preliminary study investigated tags in two social video sharing sites, YouTube and Viddler. YouTube contained many irrelevant tags because the system does not encourage users to tag their videos and does not promote tags as useful. In contrast, using tags as the sole means of categorisation in Viddler motivated users to enter a higher proportion of relevant tags. Poor tags were found in both systems, however, highlighting the need to improve video tagging. In order to give users incentives to tag videos, the VideoTag project in this thesis developed two tagging games, Golden Tag and Top Tag, and one non-game tagging system, Simply Tag, and conducted two experiments with them. In the first experiment VideoTag was a portal to play video tagging games whereas in the second experiment it was a portal to curate collections of special interest videos. Users preferred to tag videos using games, generating tags that were relevant to the videos and that covered a range of tag types that were descriptive of the video content at a predominately specific, objective level. Users were motivated by interest in the content rather than by game elements, and content had an effect on the tag types used. In each experiment, users predominately tagged videos using objective language, with a tendency to use specific rather than basic tags. There was a significant difference between the types of tags entered in the games and in Simply Tag, with more basic, objective vocabulary entered into the games and more specific, objective language entered into the non-game system. Subjective tags were rare but were more frequent in Simply Tag. Gameplay also had an influence on the types of tags entered; Top Tag generated more basic tags and Golden Tag generated more specific and subjective tags. Users were not attracted to use VideoTag by the games alone. Game mechanics had little impact on motivations to use the system. VideoTag used YouTube videos, but could not upload the tags to YouTube and so users could see no benefit for the tags they entered, reducing participation. Specific interest content was more of a motivator for use than games or tagging and that this warrants further research. In the current game-saturated climate, gamification of a video tagging system may therefore be most successful for collections of videos that already have a committed user base.

This work presents optimizations for modern capability machines and specifically for the CHERI architecture, a 64-bit MIPS instruction set extension for security, supporting fine-grained memory protection through hardware-enforced capabilities. The original CHERI model uses 256-bit capabilities to carry information required for various checks helping to enforce memory safety, leading to increased memory bandwidth requirements and cache pressure when using CHERI capabilities in place of conventional 64-bit pointers. In order to mitigate this cost, I present two new 128-bit CHERI capability formats, using different compression techniques, while preserving C-language compatibility lacking in previous pointer compression schemes. I explore the trade-offs introduced by these new formats over the 256-bit format. I produce an implementation in the L3 ISA modeling language, collaborate on the hardware implementation, and provide an evaluation of the mechanism. Another cost related to CHERI capabilities is the memory traffic increase due to capability-validity tags: to provide unforgeable capabilities, CHERI uses a tagged memory that preserves validity tags for every 256-bit memory word in a shadowspace inaccessible to software. The CHERI hardware implementation of this shadowspace uses a capability-validity-tag table in memory and caches it at the end of the cache hierarchy. To efficiently implement such a shadowspace and improve on CHERI’s current approach, I use sparse data structures in a hierarchical tag-cache that filters unnecessary memory accesses. I present an in-depth study of this technique through a Python implementation of the hierarchical tag-cache, and also provide a hardware implementation and evaluation. I find that validity-tag traffic is reduced for all applications and scales with tag use. For legacy applications that do not use tags, there is near zero overhead. Removing these costs through the use of the proposed optimizations makes the CHERI architecture more affordable and appealing for industrial adoption.

Made available in DSpace on 2016-04-26T18:11:45Z (GMT). No. of bitstreams: 1 Luciana Moherdaui.pdf: 56225694 bytes, checksum: 0ab074923ee8e7ae5e1f6842f540d10b (MD5) Previous issue date: 2012-05-25 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior / This research analyzes the news on the Web interface, although the finding can be extended to other protocols and applications (apps). The main objective is to rethink the view of news circulating in the right flow. The migration of static page culture to the culture data (BERNERS-LEE, 2009) changed the pattern of communication prevailed in the 20th century. The following terms were incorporated into the transmission, publication and reception: annotate, comment, reply, add, cut, share, download, upload, input and output. (MANOVICH, 2008, p. 226). This thesis assumes that journalism projects for the Internet are made under the logic of the printing press, with hierarchy and in columns (NELSON, 2000) when the current dynamics of the implosion of the page indicates, the loss of the standardization editorial process. In this reality, the discussion will be based from notions of relay assemblage (DELEUZE; GUATTARI, 2007, p. 180), map (DELEUZE; GUATTARI, 2006, p. 21-23) and theories of Journalism / Esta pesquisa analisa a interface jornalística na Web, embora a conclusão possa ser estendida a outros protocolos e aplicativos. O objetivo principal é repensar a exibição da notícia que circula no fluxo. A migração da cultura de página estática para a cultura de dados (BERNERS-LEE: 2009) modificou o padrão de comunicação que vigorou no século 20. Foram incorporados à transmissão, publicação e recepção os seguintes termos: anotar, comentar, responder, agregar, cortar, compartilhar, download, upload, input e output (MANOVICH: 2008, p. 226). Esta tese parte do pressuposto de que os projetos de Jornalismo para a Internet são constituídos sob a lógica do jornal impresso, com hierarquia e diagramação em colunas (NELSON: 2000) quando a dinâmica atual indica a implosão da página, a perda do processo de padronização editorial. Nesse sentido, a discussão será fundamentada a partir de noções de revezamento, agenciamento (DELEUZE; GUATTARI, 2007, p. 180), mapa (DELEUZE; GUATTARI: 2006, p. 21-23) e teorias do Jornalismo

Dada a grande quantidade de conteúdo criado por usuários na Web, uma proposta para ajudar na busca e organização é a criação de sistemas de anotações (tagging systems), normalmente na forma de palavras-chave, extraídas do próprio conteúdo ou sugeridas por visitantes. Esse trabalho aplica um algoritmo de mineração de dados em um banco de dados RDF, contendo instâncias que podem fazer referências à rede Linked Data do DBpedia, para recomendação de tags utilizando as medidas de similaridade taxonômica, relacional e literal de descrições RDF. O banco de dados utilizado é o Arquigrafia, um sistema de banco de dados na Web cujo objetivo é catalogar imagens de projetos arquitetônicos, e que permite que visitantes adicionem tags às imagens. Foram realizados experimentos para a avaliação da qualidade das recomendações de tags realizadas considerando diferentes modelos do Arquigrafia incluindo o modelo estendido do Arquigrafia que faz referências ao DBpedia. Os resultados mostram que a qualidade da recomendação de determinadas tags pode melhorar quando consideramos diferentes modelos (com referências à rede Linked Data do DBpedia) na fase de aprendizado. / Given the huge content created by users in the Web, a way to help in search and organization is the creation of tagging systems, usually in a keyword form (extracted from the Web content or suggested by users). This work applies a data mining algorithm in a RDF database, which contain instances that can reference the DBpedia Linked Data repository, to recommend tags using the taxonomic, relational and literal similarities from RDF descriptions. The database used is the Arquigrafia, a database system available in the Web which goal is to catalog architecture projects, and it allows a user to add tags to images. Experiments were performed to evaluate the quality of the tag recommendations made considering differents models of Arquigrafia\'s database, including an extended model which has references to DBpedia. The results shown that the quality of the recommendations of some tags can be improved when we consider different models (with references to DBpedia Linked Data repository) in the learning phase.

A correta adoção de medidas de manejo de resistência de insetos-pragas é motivo de preocupação constante, inclusive para as novas tecnologias disponíveis, como as plantas geneticamente modificadas para a expressão de toxinas da bactéria Bacillus thuringiensis (Bt). Portanto, para manter a eficiência das plantas-Bt comercialmente disponíveis, é preconizada a manutenção de áreas de refúgio (livres de plantas Bt) para evitar a rápida seleção de insetos resistentes. No caso de insetos polífagos, a determinação das áreas de refúgio pode levar em consideração a contribuição que fontes não-comerciais e/ou não-transgênicas têm na produção de adultos colonizadores que não sofreram seleção para o evento de transgenia de interesse. A identificação da fonte alimentar pode determinar a procedência de insetos e tornar mais eficiente e segura a implementação de zonas de refúgio e a determinação dos riscos de desenvolvimento de resistência pelo inseto-praga alvo. O objetivo deste trabalho foi o de avaliar o potencial de dois marcadores biológicos, a assinatura isotópica e a microbiota intestinal, para a identificação da fonte de alimento do imaturo pela análise de adultos de Spodoptera frugiperda (J.E. Smith) (Lepidoptera, Noctuidae), como subsídio para o monitoramento da origem de insetos migrantes em áreas de cultivo Bt para a adoção de estratégias adequadas de manejo de resistência. A análise isotópica de adultos foi realizada utilizando-se insetos criados em 12 plantas hospedeiras distintas, seis de metabolismo C3 e seis de metabolismo C4. Parte dos adultos provenientes dessa criação foram liofilizados, macerados e submetidos à análise isotópica para a determinação dos níveis de ?13C e ?15N. Amostras das plantas utilizadas na alimentação desses insetos também foram submetidas ao mesmo processo de análise. A outra parte dos adultos recémemergidos de S. frugiperda foi utilizada para a determinação da microbiota intestinal pela análise de região do gene do 16S rRNA após sequenciamento em plataforma 454. Os resultados da análise isotópica de carbono para as plantas utilizadas como fonte alimentar apresentaram médias entre -31,37? e -25,07? para plantas C3 e entre -13,03? e -12,26? para plantas C4. Adultos de S. frugiperda oriundos de lagartas criadas em plantas do grupo C3 apresentaram média de ?13C entre - 30,36? e -23,72?, enquanto aqueles do grupo C4 apresentaram média entre - 18,25? e -13,28?. O sequenciamento da microbiota via metagenômica produziu 126,970 sequências com cerca de 421 pb. O alimento influenciou substancialmente a diversidade da microbiota intestinal associada ao intestino de adultos, independentemente do metabolismo fotossintético da planta hospedeira. Análises comparativas de diversidade em que a abundância relativa dos diferentes componentes foi considerada (Unifrac ponderado) permitiu a distinção de praticamente todas as microbiotas. Foram identificadas várias UTOs exclusivamente associadas à microbiota intestinal de adultos provenientes de cada fonte de alimento, mas a maioria apresentou abundância relativa extremamente baixa. Apenas as UTOs 1199 e 2255 foram exclusivamente associados ao milho com abundância relativa superior a 2,5%. / The correct insect resistant management has been a topic of constant concern, even for the newer technologies available, such as genetically modified crops expressing Bacillus thuringiensis (Bt) toxins. The use of refuge areas with non-Bt crops to avoid the fast selection for resistant insects is proposed for maintaining the efficiency of Bt-crops. The implementation of refuge areas for polyphagous insects can consider non-commercial and non-Bt crops as sources of susceptible insects. The identification of the food source used during immature development can make the implementation of refuge areas safer and more efficient and allow for better estimates of risk assessment for insect resistance development. The objective of this study is to determine the potential use of, the isotopic signature and gut microbiota of adults as biological markers to allow for the identification of the food source during the larval stage of Spodoptera frugiperda (J.E. Smith) (Lepidoptera, Noctuidae). Adults were obtained from larval rearing on 12 food sources, six host-plants with a C3 photosynthetic metabolism and six host-plants with a C4 metabolism. Part of the adults obtained and the food source used during their immature development were lyophilized and macerated, and subjected to ?13C e ?15N isotopic analysis. The remaining adults of S. frugiperda were used to determine the composition of the gut microbiota by metagenomic analysis of the V1-V3 region of the 16S rRNA gene using a 454 sequencing platform. The carbon isotopic signatures obtained for the hostplants used as food source were between -31.37? and -25.07? for C3 plants, and - 13.03? and -12.26? for C4 plants. Adults of S. frugiperda obtained from larval rearing on C3 plants had a carbon isotopic signature between -30.36? and -23.72?, while those from C4 host-plants has between -18.25? and -13.28?. The metagenomic sequencing yielded 126,970 reads with an average of 421bp. The larval food source substantially influenced the diversity of the adult gut microbiota regardless of the plant\'s photosynthesis metabolism. Comparative analysis among gut microbiota in which the relative abundance was taken into account (weight- Unifrac) allowed the discrimination of the majority of the communities. Several OTUs were identified as exclusive to the adult gut microbiota from different food sources, but most of these OTUs were minor components of the community. OTUs 1199 and 2255, both exclusively associated with corn, were the only ones to represent at least 2.5% of their community.

Radio Frequency Identification (RFID) technology has been expanded to be used in different fields that need automatic identifying and verifying of tagged objects without human intervention. RFID technology offers a great advantage in comparison with barcodes by providing accurate information, ease of use and reducing of labour cost. These advantages have been utilised by using passive RFID tags. Although RFID technology can enhance the efficiency of different RFID applications systems, researchers have reported issues regarding the use of RFID technology. These issues are making the technology vulnerable to many threats in terms of security and privacy. Different RFID solutions, based on different cryptography primitives, have been developed. Most of these protocols focus on the use of passive RFID tags. However, due to the computation feasibility in passive RFID tags, these tags might be vulnerable to some of the security and privacy threats. , e.g. unauthorised reader can read the information inside tags, illegitimate tags or cloned tags can be accessed by a reader. Moreover, most consideration of reserchers is focus on single tag authentication and mostly do not consider scenarios that need multi-tag such as supply chain management and healthcare management. Secret sharing schemes have been also proposed to overcome the key management problem in supply chain management. However, secret sharing schemes have some scalability limitations when applied with high numbers of RFID tags. This work is mainly focused on solving the problem of the security and privacy in multi-tag RFID based system. In this work firstly, we studied different RFID protocols such as symmetric key authentication protocols, authentication protocols based on elliptic curve cryptography, secret sharing schemes and multi-tag authentication protocols. Secondly, we consider the significant research into the mutual authentication of passive RFID tags. Therefore, a mutual authentication scheme that is based on zero-knowledge proof have been proposed . The main object of this work is to develop an ECC- RFID based system that enables multi-RFID tags to be authenticated with one reader by using different versions of ECC public key encryption schemes. The protocol are relied on using threshold cryptosystems that operate ECC to generate secret keys then distribute and stored secret keys among multi RFID tags. Finally, we provide performance measurement for the implementation of the proposed protocols.

La dystrophine est une protéine cytoplasmique qui lie physiquement le cytosquelette à la matrice extracellulaire par le biais du complexe dystrophine-protéines associées (DAPC), assurant ainsi la stabilité du sarcolemme. Des mutations dans le gène DMD codant pour la dystrophine, conduisant à l’absence de la protéine, sont à l’origine de la dystrophie musculaire de Duchenne qui est une maladie liée au chromosome X. Pour mes travaux de thèse, j’ai généré et caractérisé un nouveau modèle de souris transgéniques rapportrices, dénommé DmdEGFP, qui exprime une protéine dystrophine endogène fusionnée avec la protéine fluorescente EGFP. La protéine dystrophine est liée dans sa région C-terminale qui est présente dans la majorité des isoformes. Dans le modèle, une expression forte et naturelle de l’EGFP était observée dans les muscles squelettiques, lisses, le cœur, le cerveau et l’œil, ce qui suggère un étiquetage correct de tous les isoformes de la dystrophine. La fluorescence de l’EGFP co-localisait exactement avec la dystrophine dans tous les sites. Dans le muscle squelettique, la dystrophine ainsi que d’autres protéines de la DAPC étaient exprimées dans des quantités normales et dans la bonne localisation subsarcolemmale. L’architecture du tissu musculaire squelettique était normale, suggérant que la fonction de la protéine de fusion était maintenue. In vitro, l’EGFP est également exprimée dans les fibres musculaires isolées, ainsi que dans les myotubes dérivés des cellules satellites. Par conséquent, cette nouvelle souris rapportrice de la dystrophine devient un outil important pour la visualisation directe et in vivo de l’expression de la dystrophine. / Dystrophin is a rod-shaped cytoplasmic protein that physically links the cytoskeleton to the ECM through the dystrophin-associated protein complex (DAPC), thereby providing sarcolemmal stability. Mutations in the dystrophin encoding DMD gene cause the severe X-linked disorder Duchenne muscular dystrophy. In this work a novel DmdEGFP reporter mouse that expresses a fluorescently labelled endogenous dystrophin – EGFP fusion protein was generated and characterized. The protein was tagged at the C-terminus that is present in the most dystrophin isoforms. To date, no dystrophin reporter mice exist, thus imaging is only possible by indirect antibody-mediated processing ex vivo. In DmdEGFP mice strong natural EGFP expression was observed in skeletal, smooth muscles, heart, brain and the eye and EGFP fluorescence co-localized with dystrophin at all sites suggesting proper tagging of the major dystrophin isoforms. In skeletal muscle, dystrophin as well as other proteins of the DAPC were expressed in normal quantity at correct sarcolemmal/subsarcolemmal localization. Skeletal muscle maintained normal tissue architecture, suggesting a correct function of the fusion protein. Isolated myofibers as well as satellite-cell derived myotubes expressed EGFP in vitro. Thus, the novel dystrophin reporter mouse provides a valuable tool for direct visualization of dystrophin expression.

Ventilator associated pneumonia (VAP) and acute respiratory distress syndrome (ARDS) are two respiratory conditions unique to mechanically ventilated patients. The diagnosis of these conditions, and therefore any subsequent treatment, are befuddled by uncertainty. VAP rates vary considerably according to the diagnostic or surveillance criteria used. The pathogenesis of ARDS is well understood but when the internationally agreed consensus criteria are employed, the histological hallmarks are absent about half the time, indicating a disconnection between the clinical diagnosis and what is known about the biology of this condition. It is argued that tests of biological function should be considered in addition to clinical characteristics in order to improve the utility of diagnosis. Given that the pathological sequelae of both VAP and ARDS are driven by an over exuberant host neutrophil response, the activated neutrophil was selected as a potential biological imaging target. Optical molecular imaging uses visible and near visible wavelengths from the electromagnetic spectrum to derive or visualize information based on the optical properties of the target tissue. Optical wavelengths are safe and cheap to work with, producing much higher resolution images than those relying on x-rays or gamma radiation. The imaging modality can be coupled with exogenously applied chemistry to identify specific biological targets or processes. The hypothesis that optical molecular imaging could be used to detect activated neutrophils in real time in the alveolar region of patients was tested. A bespoke optical molecular imaging agent called Neutrophil Activation Probe (NAP), designed in-house, was used to test the hypothesis. NAP is a dendrimeric compound delivered to the alveolar region of a patient in microdoses (≤100 micrograms), becoming fluorescent only on contact with activated neutrophils, and can be detected by optical endomicroscopy. Both the imaging agent and the endomicroscope are delivered to the distal lung via routine bronchoscopy. The agent was tested extensively in the laboratory to demonstrate function, specificity, and safety. Ex vivo testing took place using human and ovine lungs. A regulated dose escalation Phase I clinical trial of investigational medicinal product (CTIMP) in healthy volunteers, patients with bronchiectasis, and mechanically ventilated patients with a pulmonary infiltrate on chest radiography (NCT01532024) was designed and conducted. The aim of the Phase I study was to demonstrate the safety of the technique and to confirm proof of concept. In order to support the requirement for a technique that interrogates alveolar neutrophils two supplementary clinical studies were performed. Firstly, two VAP surveillance techniques (CDC surveillance and HELICS European VAP surveillance) were compared with clinically diagnosed VAP across consecutive admissions in two large tertiary centres for one year. Secondly, the utility of circulating neutrophils to permit discrimination between acute respiratory illnesses was examined. Blood samples from mechanically ventilated patients with and without ARDS underwent flow cytometric assessment using eight clusters of differentiation and internal markers of activation to determine neutrophil phenotype. All clinical studies received the appropriate regulatory, ethical, and/or Caldicott guardian approval prior to commencement. NAP became fluorescent only in the presence of three processes specific to neutrophil activation: active pinocytosis, progressive alkalinization of the phagolysosome, and the activity of human neutrophil elastase. High optical signal was detected following the application of NAP in the alveolar regions of explanted lungs from patients with cystic fibrosis, known to be rich in activated neutrophils. Using an ex vivo ovine lung ventilation and perfusion model optical signal was demonstrated following segmental lung injury. The safety and specificity of the technique in a small cohort of healthy volunteers and mechanically ventilated patients was demonstrated. The technique was tested on a small cohort of patients with bronchiectasis, which provided the first opportunity to obtain broncho-alveolar lavage samples for laboratory correlation. Fluorescent signal was shown in the lavaged neutrophils, labeling that could only have taken place in the alveolar region. The supportive clinical studies found the concordance between actual VAP events was virtually zero even though the reported VAP rates were similar. Furthermore, the rate at which clinicians initiate antibiotics for VAP was approximately five times higher than either surveillance VAP rate. The study of circulating neutrophils from the blood of healthy volunteers and mechanically ventilated patients with and without ARDS indicated circulating neutrophil activation phenotype was not capable of discriminating between clinically diagnosed ARDS and other acute respiratory illnesses. In summary, an ambitious programme of work was completed to develop and support an optical molecular imaging technique that meets the rigorous requirements for human application and can be applied at the bedside to yield immediate visual results. The spatiotemporal relationship of neutrophil activation in real time both in the laboratory and in volunteers and patients was visualized. The visualization of neutrophil activation at such a resolution has never been achieved before in humans, healthy or unhealthy. The Phase I study was not powered to determine utility but recruitment has begun to a Phase II CTIMP (NCT02804854) to investigate the utility, accuracy, and precision of the imaging technique in a large cohort of mechanically ventilated patients. Ultimately, it is proposed that the technique will facilitate diagnosis, stratify patients for treatment and monitor treatment response using this technique.

Autorizada por el autor para ser publicada a texto completo a contar de Noviembre de 2011 / El presente trabajo de título tuvo como objetivo la elaboración de un rediseño del proceso de cobro a usuarios de la empresa concesionaria vial Rutas del Pacífico S.A., en el que se consideró la implementación de una nueva tecnología de cobro basada en el uso del dispositivo TAG. El gobierno de Chile, en su rol de integrador de nuevas tecnologías a proyectos de infraestructura, ha solicitado a RDP, a través del Ministerio de Obras Públicas, la instalación de televías en la autopista interurbana que une Santiago con Valparaíso, conocida como Ruta 68. El proceso actual de cobro a usuarios de la autopista de RDP no considera la existencia de televías ni el cobro a través de telepeajes, por lo que, para que la implementación de la nueva tecnología de cobro sea exitosa, será necesaria la modificación de la estructura actual del proceso. El objetivo de este trabajo fue, entonces, el planteamiento de un rediseño del sistema de cobro de RDP que permita el funcionamiento eficiente de la compañía bajo el nuevo escenario tecnológico. La metodología empleada para la elaboración del rediseño corresponde a la propuesta por el profesor Oscar Barros en el libro: “Rediseño de Procesos de Negocios Mediante el Uso de Patrones”, donde el patrón de mejores prácticas utilizado se denomina Macro1. Debido a un cambio en el escenario inicial, se procedió a la elaboración de dos distintos rediseños para el proceso de cobro, los que atacan el problema desde distintos puntos de vista y proponen distintas soluciones para el mismo. Las dos alternativas de rediseño propuestas tienen sus fortalezas y debilidades, siendo la propuesta final del autor de este documento la adopción de la segunda alternativa de rediseño, que contempla la externalización de parte del proceso de cobro.

Proteins are large macromolecules that play important roles in nature. With the development of modern molecular biology techniques, protein engineering has emerged as a useful tool and found many applications in areas ranging from food industry, environmental protection, to medical and life science. Biomimetic membrane incorporates biological elements, such as proteins, to form membranes that mimic the high specificity and conductance of natural biological membranes. For any application involving the usage of proteins, the first barrier is always the production of proteins with sufficient stability, and the incorporation of proteins into the artificial matrix. This thesis contains two major parts, the first part is focused on the development and testing of method to immobilize active enzymes. The second part is devoted to study the degradation of membrane proteins in E. coli cells. In the immobilization study, Pyrophosphatase (PpaC) was chose as a model enzyme. A dual functional tag consist of histidine and methionine has been developed, in which histidine is used for purification while methionine is metabolically replaced with azidohomoalanine (AHA) for immobilization. We found that the addition of the tag and the incorporation of AHA did not significantly impair the properties of proteins, and the histidine–AHA tag can facilitate protein purification, immobilization, and labeling. This tag is expected to be useful in general for many proteins. Degradation of soluble protein has been well characterized, but the membrane protein degradation process remains elusive. SsrA tag is a well-known recognition sequence for soluble protein degradation, which marks prematurely terminated protein products translated from damaged mRNA. SsrA tagged membrane proteins was found to be substrate of a cytosolic protease complex ClpXP, which mediated complete degradation.