Levisticum officinale Koch is one of the most important plants producing essential oil. An experiment was conducted to determine the effect of drought stress on growth, total protein and essential oil content of Levisticum officinale. The experiment was conducted in a greenhouse, in a completely randomized design using 5 replications. Aridity levels of field capacity irrigation (as control), −0.6 and −1.0 MPa were applied. There was significant effect of aridity on leaf area in Levisticum officinale. The highest leaf area values were found in the lowest aridity (FC) level. The root weight and root length in the −0.6 MPa aridity level were more than that of the control aridity level, but, shoot height and shoot weight were highest in the control treatment. Total soluble protein contents under −0.6 MPa drought stress was numerically lower than that in the control treatment, but statistically there was no significant difference between protein contents in the −0.6 MPa and the control treatment. Compared to the control treatment, total soluble protein contents of Levisticum officinale were significantly decreased in the −1.0 MPa drought treatment. Essential oil content of Levisticum officinale was significantly increased in the −0.6 MPa drought treatment compared to the control. Compared to control treatment, there was a significant reduction found in essential oil content of Levisticum officinale in the −1.0 MPa aridity treatment. The results suggest that in the drought levels between −0.6 to −1.0 MPa, Levisticum officinale could be used for arid and semi-arid lands economical use.

Orientador: José Luiz Laus / Banca: Carlos Augusto Araújo Valadão / Banca: Paula Diniz Galera / Resumo: Estudou-se a eficácia do meloxicam e do carprofeno, aplicados por diferentes vias, em uveítes experimentais em cães. Realizou paracentese de câmara anterior em dois momentos (M0 e M1), com intervalo de cinco horas entre si. Em M0 e M1, colheram-se 0,2 ml de humor aquoso e determinou-se a concentração de proteína total e de prostaglandina E2 (PGE2). Em um primeiro período, constituíram-se quatro grupos (n = 5), que receberam meloxicam ao final de M0 pelas vias subcutânea (GIm), subconjuntival (GIIm) e tópica (GIIIm). Um quarto grupo não recebeu tratamento (Controle). Decorridos sete dias, os animais foram submetidos aos mesmos procedimentos adotados previamente e receberam carprofeno. Avaliação clínica foi também realizada, assim como histopatologia da conjuntiva dos animais dos grupos GIIm e GIIc. Os resultados foram avaliados estatisticamente (p LÜ 0,05). Em todos os grupos, encontrou-se aumento significativo dos níveis protéicos e de PGE2 em M1 (p < 0,001). Não se observou diferença significativa entre os grupos para os valores de proteína total e de PGE2 em M1 (p > 0,05). Observou-se correlação positiva entre proteína total e PGE2 (p < 0,05) apenas no GIm, GIc, GIIIm, GIIIc e GIIm. Exsudado inflamatório de caráter agudo e hemorragia discreta foram vistos à histopatologia após a aplicação de ambos os fármacos (p > 0,05). O meloxicam e o carprofeno foram ineficazes em inibir a síntese de PGE2 e o influxo de proteínas para a câmara anterior, por qualquer uma das vias testadas. A redução nos níveis de 44% proteínas, quando o carprofeno foi utilizado pela via tópica, sugere que por esta via, ele pode ser utilizado como adjuvante no controle da uveíte em cães. / Abstract: Efficacy of meloxican and carprofen, administered by different routes, in experimental uveitis in dogs were studied. Anterior chamber paracenteses was accomplished at two different moments (M0 and M1), with a five hour interval among them. At M0 and M1, 0,2 ml of aqueous humor were collected and total protein and prostaglandin E2 (PGE2) concentration was determined. Four groups were formed in a first period (n = 5), which received meloxican at the end of M0, by the following routes: subcutaneous (GIm), subconjunctival (GIIm), and topical (GIIIm). A fourth group that received no treatment was instituted (Control). Seven days after, animals underwent the same procedures described previously and received carprofen. Clinical evaluation was also performed, as well as conjunctival histopathology of the conjunctiva of the animals of GIIm and GIIc. Results were evaluated statistically (p LÜ 0,05). In all groups, protein and PGE2 values enhanced significantly in M1 (p < 0,001). Protein and PGE2 values, did not change significantly between groups at M1 (p > 0,05). Positive correlation among total protein and PGE2 (p < 0,05) was only noted in GIm, GIc, GIIIm, GIIIc and GIIm. Inflammatory exudate of acute character and mild hemorrhage were seen at histopathology, after both agents were administered. Meloxican and carpofen were unable to inhibit PGE2 synthesis and the protein influx to the anterior chamber by any of the tested routes. The lowering of 44% in protein levels, when carprofen was used by the topical route, suggests that by this route, it can be used as an adjuvant to control uveitis in dogs. / Mestre

Žmogaus organizmas normaliai gali egzistuoti tik tada, kai yra palaikoma pastovi vidinė organizmo terpė – homeostazė. Ji priklauso nuo normalios daugelio sistemų ir organų tarp jų ir inkstų veiklos. Šiuo tyrimu siekėme palyginti fizinio krūvio sukeltus inkstų funkcinius kitimus su sergančiųjų arterine hipertenzija patologiniais inkstų funkciniais pakitimais. Šio darbo tikslas – ištirti sportuojančių, nesportuojančių bei sergančiųjų pirmine arterine hipertenzija tiriamųjų kraujo biocheminių rodiklių, nusakančių inkstų funkciją, kaitą fizinio krūvio metu. Nurodytam tikslui pasiekti iškelti sekantys uždaviniai: 1. Palyginti sportuojančių, nesportuojančių bei sergančiųjų pirmine arterine hipertenzija kūno dydžio rodiklius. 2. Atlikti sportuojančių, nesportuojančių bei sergančiųjų pirmine arterine hipertenzija tiriamųjų kraujo biocheminių rodiklių, nusakančių inkstų funkciją, analizę ramybėje. 3. Nustatyti sportuojančių ir nesportuojančių tiriamųjų inkstų funkcinius rodiklius po fizinio krūvio ir palyginti su sergančiųjų pirmine arterine hipertenzija ramybės reikšmėmis. 4. Palyginti sportuojančių ir nesportuojančių tiriamųjų inkstų funkcinių rodiklių vidurkių tarp ramybės ir po fizinio krūvio skirtumus. 5. Išnagrinėti kreatinino klirenso kitimus. Šiame darbe kėlėme hipotezę, kad sportuojančių tiriamųjų inkstų funkciniai rodikliai po fizinio krūvio padidės labiau nei nesportuojančių tiriamųjų ir bus artimi sergančiųjų arterine hipertenzija rodikliams. Tyrime savo noru dalyvavo 10... [toliau žr. visą tekstą] / In the literature, there exists numerous studies of exercise – induced sports hematuria, proteinuria, acute renal failure following a marathon (Poortmans et al., 2001; Ayca et al., 2006; Steward, Pasen, 1980). But studies investigating the effects of exercise on blood indicators of renal function are quiet few. The aim of this study was to investigate the effects of submaximal veloergometric exercise on very important biochemical indicators of renal function – level nitrogen compounds in the blood. In venous blood samples was investigate concentrations of creatinine, urea, total protein and uric acid before and after submaximal (about 50 % VO2max) veloergometric exercise. This nitrogen compounds was studies in three groups of subjects. Methods. The study was performed on 10 trained (1 group), 10 untrained (2 group) and 10 subjects with I° hypertensive status (3 group). The age range was 20,5-21,3 yr, weight – 71,8-77,3 kg, height – 177-180 cm. All subjects volunteered to participate in the study after providing written informed consent. The study was approved in accordance with the Declaration of Helsinki. Blood samples were collected before and after the submaximal veloergometric test into vacationer tubes. Concentrations of creatinine, urea, total protein and uric acid in the serum were determined using Technician Auto Analyzer ADVIA 1650 system. Data from Submaximal ride compared using two-way repeated – measures ANOVA. Dependent t-tests were administered in the event of... [to full text]

Baculoviruses are versatile viruses that can be used as biopestisides, or for the production of recombinant protein and vaccines. Baculoviruses have also been found to be able to transfer genes to mammalian cells. This finding opened the door for the application of baculovirus vectors in human gene therapy. However, the mass production of clinical grade baculovirus vectors is challenging. Downstream processing has now become the bottle-neck of the manufacturing process. In this work, an anion exchange chromatography-based process was investigated for the purification of recombinant baculovirus vectors using a novel hydrogel based membrane (Natrix Separations Ltd.). Crude recombinant baculovirus supernatant from infected insect cell cultures was first subjected to a clarification process consisting of centrifugation and filtration. The pH of the viral solution was adjusted and then passed through a fast protein liquid chromatography system consisting of the ion exchange membrane. After washing weakly bound impurities, the captured baculoviruses are recovered by an elution step. Overall, baculoviruses strongly associated with the membrane; however, this interaction which was much physical as it was chemical, could not be entirely reversed and baculovirus was lost in the process. Product purity has also been evaluated and up to 85% of total protein reduction was determined. The significant losses of baculovirus observed have indicated major limitations in using this membrane for the purification of baculovirus.

Šio darbo tiklas buvo nustatyti senų arklių sveikatos palaikymui naudojamų gliukokortikoidų poveikį kraujo biocheminiams rodikliams ir įvertinti kaip pasikeičia kraujo gliukozės, bendrųjų baltymų ir trigliceridų koncentracija kraujyje panaudojus ilgo ir trumpo veikimo gliukokortikoidų dozės. Tyrimams buvo atrinkta 18 senų sportinių veislinių žirgų, kurie anksčiau intensyviai buvo naudojami sportinėje veikloje. Visi jie eržilai, turintys negalavimų susijusių su senomis sąnarių ir nugaros traumomis. Sudarytos trys žirgų grupės. I gr. žirgams buvo taikomas palaikomasis gydymas naudojat trumpo veikimo gliukokortikoidų preparatą po 2,0 ml / 100 kg svorio. Organizme preparatas išlieka iki 24 val. II gr. žirgams buvo naudojamas ilgai veikiantis gliukokortikoidų preparatas po 10,0 ml neatsižvelgiant į arklio svorį. III gr. – kontrolinė. I ir II grupių žirgų kraujas buvo imamas 6 kartus, o kontrolinės – vieną kartą. Prieš preparato suleidimą ir po suleidimo praėjus 1, 2, 3, 4 ir 7 d. ir nustatoma gliukozės, bendrųjų baltymų ir trigliceridų koncentracija. Tyrimo metu nustatyta, kad naudojant preparatus ilgo ir trumpo veikimo su gliukokortikoidais, pakinta medžiagų apykaita ir padidėja gliukozės, trigliceridų ir bendrųjų baltymų koncentracija kraujyje. / Aim of the study: The aim of this study was to establish, how the results of old horses biochemistry blood tests, such as glucose, total proteins and triglycerides change after the use of long acting or short acting glucocorticoids. Methods: There were 18 old sport horses in this study. All of them were stallions, who had old joint and back traumas. The horses were divided into the three groups: horses from the first group were treated with the short acting glucocorticoids – 2,0 ml / 100 kg. It remains in the body for up to 24 hours. Horses from the second group were treated with long acting glucocorticoids – 10,0 ml. It remains in the body for up to 4 days. The third was control group of horses. Blood samples from the horses of the first and second group were taken 6 times. Blood samples from the control group were taken once. Before the injection and after the injection of glucocorticoids, the concentration of glucose, total protein and triglycerides were carried out. Results: The test results showed, that the use of long and short acting glucocorticoids can change the metabolism. After the use of glucocorticoids, the concentration of glucose, triglycerides and total proteins have increased.

Plasma protein shave many important functions in the organism. Gamma globulins are carriers of immunoglobulins which play animportant role in the immune response. Their contentis primarily given by the health burden of the organism. The aimofthe study was to determine the individual protein fractions in the blood plasma of ewes and lambs, comparemutual relations between total plasma proteins and thein variol factions and assess the concentrations of individual plasma proteins, mainly globulin, in connection with the aktivity of the thyroidgland, physiological state, and increments. The sampling was carried out in the spring (25.3.) and the autumn (14.10) 2013. The individual protein fractions were determined from the serum by the means of electrophoresis. The results show that the concentration of proteins in the blood plasma of bothewes and ewe lambs and ram lambs was high due to haemoconcentration, heat stress during sampling, grazing young green forage, comprising a large number of protein aceous substances, and increasing demands on energy for milk production, especially in the early stagesoflactation. Theconcentrationofproteins in theblood plasma oflambspertains to thegrowthproduction. It establishes a correlation between the concentration of plasma proteins and the aktivity of the thyroidgland. It also confirmes higher concentrations of plasma proteins of lambs correlating to thein higher daily gain. Due to the high concentration of total protein in the plasma, the level of its fractions was high as well. After the conversion to a percentage, the level of - globulins in blood plasma in all categories was within normal limits in the range of 14-27%. The concentration of - globulins in blood plasma increased in relation to the parasitological findings coccidia of the genus Eimeria and gastrointestinal nematodes. Relations between the kontent of plasma proteins in the blood of the lambs and ewe sobserved were in most casespositive. There was a strong dependence between the total protein and globulins. Even among other fractions of plasma proteins and globulins correlation coefficient was almou always positive.

The aim of the thesis was to evaluate the influence of selected factors on the level of passive immunization of calves (expressed in total protein, g/l in blood serum) in selected beef cattle breeding. In the monitored period 2014-2016, a total of 205 samples of calf serum were evaluated. The selected factors were: the length of dry period and parity, colostrum quality, the time of first intake of colostrum after birth, the weight and sex of the calf. An adequate level of passive immunity was found in 90.2% of calves. This result was also reflected in low calf mortality rates in monitored years: 1.16% (2014), 1.15% (2015) and 1.23% (2016). At the level of passive immunization, the time of the first colostrum intake (P < 0,001), parity (P = 0,0002), colostrum quality (P = 0,0061) and the length of dry period (P = 0,0008) were the most important factors. The other evaluated factors (sex and birth weight of calves) were not statistically significant. Colostrum period management is essential for the rearing of healthy calves and profitability of breeding.

Made available in DSpace on 2014-06-11T19:23:42Z (GMT). No. of bitstreams: 0 Previous issue date: 2007-07-27Bitstream added on 2014-06-13T20:48:19Z : No. of bitstreams: 1 ribeiro_ap_me_jab.pdf: 821460 bytes, checksum: 9540c58da44188e3ae8a41593d305a9c (MD5) / Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) / Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) / Estudou-se a eficácia do meloxicam e do carprofeno, aplicados por diferentes vias, em uveítes experimentais em cães. Realizou paracentese de câmara anterior em dois momentos (M0 e M1), com intervalo de cinco horas entre si. Em M0 e M1, colheram-se 0,2 ml de humor aquoso e determinou-se a concentração de proteína total e de prostaglandina E2 (PGE2). Em um primeiro período, constituíram-se quatro grupos (n = 5), que receberam meloxicam ao final de M0 pelas vias subcutânea (GIm), subconjuntival (GIIm) e tópica (GIIIm). Um quarto grupo não recebeu tratamento (Controle). Decorridos sete dias, os animais foram submetidos aos mesmos procedimentos adotados previamente e receberam carprofeno. Avaliação clínica foi também realizada, assim como histopatologia da conjuntiva dos animais dos grupos GIIm e GIIc. Os resultados foram avaliados estatisticamente (p LÜ 0,05). Em todos os grupos, encontrou-se aumento significativo dos níveis protéicos e de PGE2 em M1 (p < 0,001). Não se observou diferença significativa entre os grupos para os valores de proteína total e de PGE2 em M1 (p > 0,05). Observou-se correlação positiva entre proteína total e PGE2 (p < 0,05) apenas no GIm, GIc, GIIIm, GIIIc e GIIm. Exsudado inflamatório de caráter agudo e hemorragia discreta foram vistos à histopatologia após a aplicação de ambos os fármacos (p > 0,05). O meloxicam e o carprofeno foram ineficazes em inibir a síntese de PGE2 e o influxo de proteínas para a câmara anterior, por qualquer uma das vias testadas. A redução nos níveis de 44% proteínas, quando o carprofeno foi utilizado pela via tópica, sugere que por esta via, ele pode ser utilizado como adjuvante no controle da uveíte em cães. / Efficacy of meloxican and carprofen, administered by different routes, in experimental uveitis in dogs were studied. Anterior chamber paracenteses was accomplished at two different moments (M0 and M1), with a five hour interval among them. At M0 and M1, 0,2 ml of aqueous humor were collected and total protein and prostaglandin E2 (PGE2) concentration was determined. Four groups were formed in a first period (n = 5), which received meloxican at the end of M0, by the following routes: subcutaneous (GIm), subconjunctival (GIIm), and topical (GIIIm). A fourth group that received no treatment was instituted (Control). Seven days after, animals underwent the same procedures described previously and received carprofen. Clinical evaluation was also performed, as well as conjunctival histopathology of the conjunctiva of the animals of GIIm and GIIc. Results were evaluated statistically (p LÜ 0,05). In all groups, protein and PGE2 values enhanced significantly in M1 (p < 0,001). Protein and PGE2 values, did not change significantly between groups at M1 (p > 0,05). Positive correlation among total protein and PGE2 (p < 0,05) was only noted in GIm, GIc, GIIIm, GIIIc and GIIm. Inflammatory exudate of acute character and mild hemorrhage were seen at histopathology, after both agents were administered. Meloxican and carpofen were unable to inhibit PGE2 synthesis and the protein influx to the anterior chamber by any of the tested routes. The lowering of 44% in protein levels, when carprofen was used by the topical route, suggests that by this route, it can be used as an adjuvant to control uveitis in dogs.

Calves are born agammaglobulinemic and rely on colostrum consumption for the transfer of maternal passive immunity. Calves that fail to absorb adequate amounts of maternal antibodies from colostrum are commonly referred to as having failed transfer of passive immunity (FTPI). The overall aim of this dissertation was to explore the usefulness of FTPI testing in neonatal beef calves to predict their risk for subsequent illness or death. The objectives were to evaluate the impact of FTPI on pre-weaning disease in beef and dairy calves, quantify and compare the variance in IgG concentrations measured by radial immunodiffusion and serum total protein (STP) values measured by optical refractometry, and evaluate the correlation between herd-level prevalence of FTPI and herd-level prevalence of pre-weaning disease in beef calves. Evaluation of literature relevant to FTPI was compiled and assessed to quantify the impact of FTPI on pre-weaning disease in beef and dairy calves. A series of randomized trials were used to evaluate the variance in IgG concentrations and STP values from banked serum. Health records from multiple farms and IgG results were used to evaluate the relationship between FTPI and disease at the individual and herd-level. Failed transfer of passive immunity had a variable association with pre-weaning disease in beef and dairy calves. IgG concentrations were less precise than STP values especially when dilution was required. IgG concentrations and STP values were associated with an increased risk of disease in pre-weaned beef calves, but FTPI cut-off values poorly classified the risk for subsequent disease. The proportion of calves with FTPI was not correlated with the proportion of calves that developed pre-weaning disease. Using a single immunological factor, such as IgG concentration or STP, to predict disease results in the misclassification of disease risk and does not consider additional component causes of disease.

No description available.