This diploma thesis is dealing with methods of identification genetic variability in sequencing data. The resarch is targeted to bacterial strains of Treponema pallidum. The sequencing was performed by Illumina platform. There is a proposition of method to identificate variable spots in resequenced genomes and their analysis and comparation across all processed genomes.

Magister Chirurgiae Dentium (MChD) / There is an absence of both dental services and systematic planning to meet the oral health needs of the Black* population ~f greater Cape Town. Little epidemiological data exists upon which such planning can be based. This study describes the prevalence and treatment need related to tooth decay and periodontal disease ofaXhosa-speaking* squatter community on the outskirts of Cape Town. An age and sex stratified sample of 290 adults attending the SACLA clinic in Crossroads were examined. Examiner variability was measured by a percentage intra-examiner agreement for the DMFT of 95% and for the CPITN 84%. Cohen's kappa statistic, for tooth-specific caries detection errors was k = 0.877. The mean DMFT was 11.8 and varied little with sex or age below 55 years. After this age, the DMFT climbs steeply due largely to the rapid increase in the M value (missing teeth). The results show that for every tooth needing to be extracted, two teeth per subject required a restoration. Only three subjects already had some restorations. Periodontal health was reflected by a high prevalence of calculus (TN2 = 99%; MNS = 5.2) for the whole sample. Deep pockets were detected in 13% of those aged between 15 and 29 years, but only at a relatively low intensity (MNS = 0.1). This prevalence reached a high 60% for those aged between 45 and 64 years (MNS = 1.7). All subjects require oral hygiene instruction and gross scaling in at least four sextants, according to CPITN criteria. In conclus~on it is noted that there is a shortage of relevant epidemiological information necessary to the planning of oral health services to improve the oral health of the Xhosa-speaking community in the Western Cape. Caries prevalence rates are already high in young adults and a high tooth mortality rate and an absence of fillings, suggests that extraction is the only form of treatment made available to this community. The absence of appropriate prevention strategies such as water fluoridation is reflected in these results. The existence of small amounts of severe periodontal disease in young adults is of concern. The high prevalence of mild (and preventable) periodontal disease, seems to reflect a low awareness of the condition and/or a lack of resources to control it. It is no coincidence that such poor oral health was observed in this, a poor, peri-urban squatter community. This study, serves as a sad reminder of the maldistribution of oral health and socia-economic resources in South Africa. The socia-economic and political character of this community is reflected by the epidemiological picture of oral health observed in the study. It is clear that further data must be collected, especially a clear assessment of community-expressed needs. Active planning must take place urgently to integrate oral health with Primary Health Care to rectify the serious misuse and maldistribution of oral health resources required to improve the oral health of this population.

No / Aim: A diagnostic test that could detect Treponema pallidum antigens in urine would facilitate the prompt diagnosis of syphilis. Materials & methods: Urine from 54 individuals with various clinical stages of syphilis and 6 controls were pooled according to disease stage and interrogated with complementary mass spectrometry techniques to uncover potential syphilis biomarkers. Results & conclusion: In total, 26 unique peptides were uncovered corresponding to four unique T. pallidum proteins that have low genetic sequence similarity to other prokaryotes and human proteins. This is the first account of direct T. pallidum protein detection in human clinical samples using mass spectrometry. The implications of these findings for future diagnostic test development is discussed. Data are available via ProteomeXchange with identifier PXD009707.

La parodontite est une infection polymicrobienne qui entraîne une inflammation du parodonte et provoque une destruction irréversible des tissus de soutien de la dent. Le but de cette étude était d'évaluer les concentrations des cytokines IL-18, IL-1β, IL-10, TNFα ainsi que celles des récepteurs Toll de type 2 (Toll-like receptor 2, TLR2) et 4 (TLR4) solubles dans le fluide créviculaire gingivale (FCG) et la salive d'une cohorte de patients atteints de parodontite. Ces concentrations ont été corrélées avec les paramètres cliniques de la parodontite et la quantité des bactéries Porphyromonas gingivalis, Treponema denticola et Fusobacterium nucleatum retrouvées chez les participants à l'étude. Les résultats obtenus ont révélé que les taux d'IL-18, d'IL-1β et de TLR4 dans le FCG sont plus élevés chez les sujets atteints de parodontite par rapport à ceux exempts de la maladie et que la sévérité de la maladie parodontale est associée à des concentrations élevées d'IL-1β et de TLR4 chez les sujets malades.

INTRODUÇÃO: Infecções pelo HIV, HBV, HCV e Treponema pallidum encontram no sistema prisional um ambiente favorável para suas propagações e representam grave problema de saúde pública. Neste estudo buscamos conhecer o perfil epidemiológico dos reeducandos de uma unidade prisional e a dinâmica do seu comportamento associado às infecções estudadas. Foram medidas as prevalências dessas infecções e suas coinfecções, investigados os potenciais fatores de risco, assim como medido o padrão de uso de drogas no presídio com ênfase no crack. MÉTODOS: Estudo epidemiológico transversal realizado de fevereiro a dezembro de 2007, na Penitenciária I de São Vicente. Realizada uma análise descritiva utilizando medidas de frequência, médias e desvio padrão. Foram considerados apenas os diagnósticos sorológicos para definição das infecções. Utilizado o OR (odds ratio) como medida de associação com nível de significância de 5% (?). Utilizada a regressão logística para estimar OR ajustado por algumas variáveis. RESULTADOS: Participaram das entrevistas 546 (84,1%) reeducandos e 514 (94,1%) realizaram as sorologias. Apresentaram um perfil jovem com idade média de 29,8 anos. 52,0% (283) referiram relacionamento conjugal com média de dois filhos. Declararam ser de cor parda 51,5% (280). O tempo médio de prisão foi de 10,5 anos. As prevalências identificadas foram: HIV 1,8% [IC95% = 0,1- 3,3], HBV 21,0% [IC95% = 17,8-25,1], HCV 5,3% [IC95% = 3,5-7,6], e 5,3% [IC95% =3,5-7,6] para a infecção pelo Treponema pallidum. Os fatores de risco associados com a infecção pelo HIV foram: uso de droga injetável (OR=15,38), > 30 anos (OR=13,3), uso de cocaína na vida (OR= 5,36) e uso de crack na vida (OR= 5,21). Nas análises multivariadas as variáveis que se mantiveram associadas com o HBV foram: uso de droga injetável (OR=3,36), ter referido DST (OR= 2,28), > de 30 anos (OR=1,86) e mais de cinco anos de prisão (OR= 2,17); com o HCV foram: uso de droga injetável (OR=9,65), uso de maconha na prisão (OR=2,91) e idade >30 anos (OR=8,41); com o Treponema pallidum foram: relação homossexual (OR=11,92) e ter referido sífilis (OR=10,88). As prevalências das coinfecções foram: 0,8% [IC95%= 0,2-2,0] para HIV/HBV, 1,4% [IC95%=0,5-2,8], para HBV/Treponema pallidum, 1,8% [IC95%= 0,8-3,3] para HBV/HCV, e para a tripla infecção 0,4% [IC95%= 0,5-1,4]. Confirmaram o uso de crack na vida 25,0% (136) dos entrevistados. Idade média de início de uso de drogas ilícitas foi de 15,4 anos. Na prisão, o uso de álcool foi relatado por 8,4% (45), tabaco por 62,0% (318), maconha por 36,5 % (194), cocaína por 9,0% (48), e crack por 11,7% (15), sendo seu uso diário referido por 2,3% (3). Uso de drogas injetáveis na vida foi referido por 5,9% (32) e nenhum uso nos últimos seis meses. As situações de violência relacionadas com drogas para 14,3% (28) foram de ameaças de morte, 16,7% (57) agressões físicas e 27,3% (3) sofreram agressão sexual. Para 15,8% (30) o crack esteve relacionado com estas situações. O seu uso com outras drogas foi referido por 41,5% (54). CONCLUSÃO: As prevalências encontradas na população confinada continuam maiores que as observadas na população geral. O consumo de drogas e praticas sexuais desprotegidas foram mantidas no período de confinamento. O tempo de confinamento mostrou-se fator importante na análise de risco. Os fatores de riscos identificados aparecem como importantes indicadores para a estruturação de estratégias de controle dessas infecções junto à população confinada. / INTRODUCTION: Infection by HIV, HBV, HCV and Treponema pallidum are common in the prison system due to an environment favorable to their propagation and represent a serious public health problem. This study aims to understand the epidemiological profile of the inmates in a prison unit and the behaviors associated with the infectious diseases studied. We estimated the prevalence of these infections and their co-infections, investigated potential risk factors, as well as identified the pattern of drug use in prison, especially crack use. METHODS: A cross-sectional epidemiological study conducted from February to December 2007 at the São Vicente Penitentiary . A descriptive analysis using frequency measures, means and standard deviations was conducted. We considered only the setting for serological diagnosis of infections. Odds ratio (OR) with a significance level of 5% (?) was used as a measure of association and a logistic regression was applied to estimate adjusted OR for some variables. RESULTS: A total of 546 prisoners were interviewed (84.1%). Of these, 514 (94.1%) underwent serological analysis. Results showed a profile of young men with an average age of 29.8 years. Fifty-two percent (283) reported marital relationship with an average of two children, and 51.5% (280) reported being of mixed ethnicity. The average time of arrest was 10.5 years. The prevalences were identified: HIV 1.8% [95% CI = 0.1 - 3.3], HBV 21.0% [95% CI 17.8 to 25.1], HCV 5.3% [95% CI = 3.5 to 7.6] and 5.3% [95% CI = 3.5 to 7.6] for infection with Treponema pallidum. The risk factors associated with HIV infection were injected-drug use (OR = 15.38), > 30 years (OR = 13.3), cocaine use (OR = 5.36) and use of crack during lifetime (OR = 5.21). According to multivariate analyzes, variables associated with HBV were: injected-drug use (OR = 3.36), reported any STD (OR = 2.28), > 30 years (OR = 1.86) and more than five years in prison (OR = 2.17); the variables associated with HCV were: injected-drug use (OR = 9.65), marijuana use in prison (OR = 2.91) and age> 30 years (OR = 8.41); the variables associated with Treponema pallidum were: homosexual intercourse (OR = 11.92) and have referred syphilis (OR = 10.88). The prevalence of co-infections were 0.8% [95% CI = 0.2 to 2.0] for HIV / HBV, 1.4% [95% CI = 0.5 to 2.8] for HBV / Treponema pallidum, 1.8% [95% CI = 0.8 to 3.3] for HBV / HCV infection and for the triple infection 0.4% [95% CI = 0.5 to 1.4]. Average age of onset of illicit drug use was 15.4 years. The use of crack during lifetime was reported by 25.0% (136) of respondents. In prison, alcohol use was reported by 8.4% (45), tobacco by 62.0% (318), marijuana by 36.5% (194), 9.0% for cocaine (48), and crack by 11.7% (15), with the daily use of crack reported by 2.3% (3). Injected-drug use during lifetime was reported by 5.9% (32), but no use in the last six months was reported. Drug-related violence episodes reported were death threats 14.3% (28), 16.7% (57) assaults and 27.3% (3) suffered sexual assault. To 15.8% (30) of the respondents, crack use was associated with violence episodes. The use of crack-cocaine in association with other drugs was reported by 41.5% (54). CONCLUSION: The prevalence found in the confined population is still larger than those observed in the general population. Drug use and unprotected sexual practices were maintained during the period of confinement. The confinement time proved to be an important factor in the analysis of risk of infection. The identified risk factors appear to be important indicators for developing strategies to control these infections in the prison environment.

INTRODUÇÃO: Infecções pelo HIV, HBV, HCV e Treponema pallidum encontram no sistema prisional um ambiente favorável para suas propagações e representam grave problema de saúde pública. Neste estudo buscamos conhecer o perfil epidemiológico dos reeducandos de uma unidade prisional e a dinâmica do seu comportamento associado às infecções estudadas. Foram medidas as prevalências dessas infecções e suas coinfecções, investigados os potenciais fatores de risco, assim como medido o padrão de uso de drogas no presídio com ênfase no crack. MÉTODOS: Estudo epidemiológico transversal realizado de fevereiro a dezembro de 2007, na Penitenciária I de São Vicente. Realizada uma análise descritiva utilizando medidas de frequência, médias e desvio padrão. Foram considerados apenas os diagnósticos sorológicos para definição das infecções. Utilizado o OR (odds ratio) como medida de associação com nível de significância de 5% (?). Utilizada a regressão logística para estimar OR ajustado por algumas variáveis. RESULTADOS: Participaram das entrevistas 546 (84,1%) reeducandos e 514 (94,1%) realizaram as sorologias. Apresentaram um perfil jovem com idade média de 29,8 anos. 52,0% (283) referiram relacionamento conjugal com média de dois filhos. Declararam ser de cor parda 51,5% (280). O tempo médio de prisão foi de 10,5 anos. As prevalências identificadas foram: HIV 1,8% [IC95% = 0,1- 3,3], HBV 21,0% [IC95% = 17,8-25,1], HCV 5,3% [IC95% = 3,5-7,6], e 5,3% [IC95% =3,5-7,6] para a infecção pelo Treponema pallidum. Os fatores de risco associados com a infecção pelo HIV foram: uso de droga injetável (OR=15,38), > 30 anos (OR=13,3), uso de cocaína na vida (OR= 5,36) e uso de crack na vida (OR= 5,21). Nas análises multivariadas as variáveis que se mantiveram associadas com o HBV foram: uso de droga injetável (OR=3,36), ter referido DST (OR= 2,28), > de 30 anos (OR=1,86) e mais de cinco anos de prisão (OR= 2,17); com o HCV foram: uso de droga injetável (OR=9,65), uso de maconha na prisão (OR=2,91) e idade >30 anos (OR=8,41); com o Treponema pallidum foram: relação homossexual (OR=11,92) e ter referido sífilis (OR=10,88). As prevalências das coinfecções foram: 0,8% [IC95%= 0,2-2,0] para HIV/HBV, 1,4% [IC95%=0,5-2,8], para HBV/Treponema pallidum, 1,8% [IC95%= 0,8-3,3] para HBV/HCV, e para a tripla infecção 0,4% [IC95%= 0,5-1,4]. Confirmaram o uso de crack na vida 25,0% (136) dos entrevistados. Idade média de início de uso de drogas ilícitas foi de 15,4 anos. Na prisão, o uso de álcool foi relatado por 8,4% (45), tabaco por 62,0% (318), maconha por 36,5 % (194), cocaína por 9,0% (48), e crack por 11,7% (15), sendo seu uso diário referido por 2,3% (3). Uso de drogas injetáveis na vida foi referido por 5,9% (32) e nenhum uso nos últimos seis meses. As situações de violência relacionadas com drogas para 14,3% (28) foram de ameaças de morte, 16,7% (57) agressões físicas e 27,3% (3) sofreram agressão sexual. Para 15,8% (30) o crack esteve relacionado com estas situações. O seu uso com outras drogas foi referido por 41,5% (54). CONCLUSÃO: As prevalências encontradas na população confinada continuam maiores que as observadas na população geral. O consumo de drogas e praticas sexuais desprotegidas foram mantidas no período de confinamento. O tempo de confinamento mostrou-se fator importante na análise de risco. Os fatores de riscos identificados aparecem como importantes indicadores para a estruturação de estratégias de controle dessas infecções junto à população confinada. / INTRODUCTION: Infection by HIV, HBV, HCV and Treponema pallidum are common in the prison system due to an environment favorable to their propagation and represent a serious public health problem. This study aims to understand the epidemiological profile of the inmates in a prison unit and the behaviors associated with the infectious diseases studied. We estimated the prevalence of these infections and their co-infections, investigated potential risk factors, as well as identified the pattern of drug use in prison, especially crack use. METHODS: A cross-sectional epidemiological study conducted from February to December 2007 at the São Vicente Penitentiary . A descriptive analysis using frequency measures, means and standard deviations was conducted. We considered only the setting for serological diagnosis of infections. Odds ratio (OR) with a significance level of 5% (?) was used as a measure of association and a logistic regression was applied to estimate adjusted OR for some variables. RESULTS: A total of 546 prisoners were interviewed (84.1%). Of these, 514 (94.1%) underwent serological analysis. Results showed a profile of young men with an average age of 29.8 years. Fifty-two percent (283) reported marital relationship with an average of two children, and 51.5% (280) reported being of mixed ethnicity. The average time of arrest was 10.5 years. The prevalences were identified: HIV 1.8% [95% CI = 0.1 - 3.3], HBV 21.0% [95% CI 17.8 to 25.1], HCV 5.3% [95% CI = 3.5 to 7.6] and 5.3% [95% CI = 3.5 to 7.6] for infection with Treponema pallidum. The risk factors associated with HIV infection were injected-drug use (OR = 15.38), > 30 years (OR = 13.3), cocaine use (OR = 5.36) and use of crack during lifetime (OR = 5.21). According to multivariate analyzes, variables associated with HBV were: injected-drug use (OR = 3.36), reported any STD (OR = 2.28), > 30 years (OR = 1.86) and more than five years in prison (OR = 2.17); the variables associated with HCV were: injected-drug use (OR = 9.65), marijuana use in prison (OR = 2.91) and age> 30 years (OR = 8.41); the variables associated with Treponema pallidum were: homosexual intercourse (OR = 11.92) and have referred syphilis (OR = 10.88). The prevalence of co-infections were 0.8% [95% CI = 0.2 to 2.0] for HIV / HBV, 1.4% [95% CI = 0.5 to 2.8] for HBV / Treponema pallidum, 1.8% [95% CI = 0.8 to 3.3] for HBV / HCV infection and for the triple infection 0.4% [95% CI = 0.5 to 1.4]. Average age of onset of illicit drug use was 15.4 years. The use of crack during lifetime was reported by 25.0% (136) of respondents. In prison, alcohol use was reported by 8.4% (45), tobacco by 62.0% (318), marijuana by 36.5% (194), 9.0% for cocaine (48), and crack by 11.7% (15), with the daily use of crack reported by 2.3% (3). Injected-drug use during lifetime was reported by 5.9% (32), but no use in the last six months was reported. Drug-related violence episodes reported were death threats 14.3% (28), 16.7% (57) assaults and 27.3% (3) suffered sexual assault. To 15.8% (30) of the respondents, crack use was associated with violence episodes. The use of crack-cocaine in association with other drugs was reported by 41.5% (54). CONCLUSION: The prevalence found in the confined population is still larger than those observed in the general population. Drug use and unprotected sexual practices were maintained during the period of confinement. The confinement time proved to be an important factor in the analysis of risk of infection. The identified risk factors appear to be important indicators for developing strategies to control these infections in the prison environment.

Periodontal disease (PD) is a polymicrobial infection characterized by inflammation of the gingiva, alveolar bone resorption, and tooth loss (edentulism). Treponema denticola along with Porphyromonas gingivalis and Tannerella forsythia are among the “Red Complex” and are main etiological agents in PD. Treponemes are a member of the Spirochaeta phylum and are obligate anaerobes, that express pyruvate ferredoxin oxidoreductase (PFOR). The enzyme catalyzes the oxidation of pyruvate to acetyl-CoA and reduced ferredoxin. Amixicile is a novel bacteriostatic derivative of nitazoxanide and an inhibitor of PFOR. In light of the fact that Treponemes express PFOR, this study was conducted to investigate the susceptibility of oral Treponemes to AMX. All oral Treponemes tested were susceptible to AMX and the MIC values were determined ranging of 1.5-4.5 μg mL-1 for an initial starting cell concentration of 1.9x106 cells mL-1. Other potentially therapeutic effects for AMX for T. denticola were investigated: motility, hydrogen sulfide production, and serum sensitivity. AMX reduced overall spirochete motility by 50% at sub-MIC concentrations. There was a dose dependent decrease in H2S production in T. denticola at sub-MIC and MIC values. Furthermore, prior exposure of AMX led to increases in serum sensitivity. Taking into account the fact that other periodontal red complex bacteria express PFOR, AMX could serve as a new selective adjunctive treatment for periodontal disease.

Submitted by Cássio da Cruz Nogueira (cassionogueirakk@gmail.com) on 2017-05-05T11:38:15Z No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Tese_PeriodontiteOvinosEstado.PDF: 11749160 bytes, checksum: 07582d26b606607e8e1786f83ce34921 (MD5) / Approved for entry into archive by Edisangela Bastos (edisangela@ufpa.br) on 2017-05-09T20:27:18Z (GMT) No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Tese_PeriodontiteOvinosEstado.PDF: 11749160 bytes, checksum: 07582d26b606607e8e1786f83ce34921 (MD5) / Made available in DSpace on 2017-05-09T20:27:18Z (GMT). No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Tese_PeriodontiteOvinosEstado.PDF: 11749160 bytes, checksum: 07582d26b606607e8e1786f83ce34921 (MD5) Previous issue date: 2015-01-29 / O presente trabalho relata os aspectos epidemiológicos, clinico-patológicos e bacteriológicos do primeiro registro de periodontite em ovinos no Brasil, ocorrido em uma propriedade rural no município de Benevides, Para. O surto ocorreu aproximadamente um mês após o pastejo na área de Panicum maximum cv. Massai, a qual sofreu praticas agrícolas, quando foi observado aumento de volume na mandíbula em alguns animais, em sua maioria com idade acima de 36 meses. Foi realizado o exame clinico extra-oral dos 545 ovinos e verificou-se aumento de volume da mandíbula em 3,7%. Os animais acometidos apresentavam baixo escore corporal, pelos arrepiados e sem brilho, alguns com afrouxamento e perda dos dentes pré-molares e molares inferiores e superiores, formação de abscesso e fístula no local acometido, demonstração de dor a palpação e dificuldade de mastigação. Das 39 cabeças de animais jovens analisadas no exame macroscópico post-mortem, 51,3% apresentavam lesões periodontais e das 38 analisadas após a maceração, 73,7% também apresentavam lesões. Das cabeças com lesões no exame post-mortem, em 45% as lesões encontravam-se na maxila, 15% na mandíbula e 40% nas duas estruturas (maxila e mandíbula). Nas cabeças com lesões após a maceração, 50% encontravam-se na maxila e 50% na maxila e mandíbula. Das 17 cabeças de animais adultos analisadas no exame post-mortem e após a maceração, todas apresentavam lesões periodontais. No exame post-mortem, 11,8% apresentavam lesões na mandíbula e 88,2% nas duas estruturas; após a maceração, 5,9% na maxila, 11,8% na mandíbula e 82,3% nas duas estruturas. Os achados histopatológicos revelaram processo inflamatório piogranulomatoso. Para caracterização da microbiota bacteriana subgengival de 14 ovinos com periodontite foi realizada a Reação em Cadeia da Polimerase (PCR) para pesquisa de micro-organismos pertencentes ao complexo vermelho de Socransky (Porphyromonas gingivalis, Tannerella forsythia e Treponema denticola) e outros possíveis periodontopatogenos Gram-negativos e Gram-positivos. Em 50% das amostras foi possível identificar Porphyromonas gingivalis, em 92,8% Tannerella forsythia e em 78,5% Treponema denticola. Os três periodontopatogenos pertencentes ao complexo vermelho ocorreram concomitantemente em 42,8% das amostras. Foram identificados ainda em pelo menos um animal examinado Campylobacter rectus, Eikenella corrodens, Enterococcus faecium, Fusobacterium nucleatum, Prevotella intermedia, Prevotella loeschii e Prevotella nigrescens. Não foram detectados nas 14 amostras Aggregatibacter actinomycetemcomitans, Dialister pneumosintes, Enterococcus faecalis e Porphyromonas gulae. Os resultados permitem concluir que a periodontite ovina ocorrida no município de Benevides – PA possui etiologia infecciosa e acometeu um significativo numero de animais, diversos com abaulamento da mandíbula; teve alta incidência nos jovens e envolveu a totalidade dos animais adultos examinados post-mortem e após a maceração. / This paper describes the epidemiological, clinical, pathological and bacteriological the first periodontitis record in sheep in Brazil occurred in a rural property in the municipality of Benevides, Para. The outbreak occurred about a month after grazing in the area of Panicum maximum cv. Massai, which suffered agricultural practices, there was swelling in the jaw in a few animals, mostly those over the age of 36 months. The extraoral examination was performed in 545 sheep and there was swelling of the jaw in 3.7% of them. Clinical signs were low body score, rough hair coat and dull, some relaxation and loss of premolars and upper and lower molar teeth, abscess and fistula formation in the affected site, demonstration of pain on palpation and difficulty chewing. Between the 39 heads of young animals analyzed in the post-mortem examination, 51.3% had lesions somewhere and between the 38 analyzed after maceration, 73.7% also had injuries. 45% of the lesions from the 39 heads were in the maxilla, 15% in the mandible and 40% in both structures (maxilla and mandible) and from those heads with injuries observed after maceration 50% of the lesions were in the maxilla and 50% in the maxilla and mandible. Between the 17 heads of adult animals analyzed in the post-mortem examination and after maceration all had lesions somewhere. In the post-mortem examination, 11.8% had lesions in the mandible and 88.2% in both structures; after maceration, 5.9% were in the maxilla, 11.8% in the mandible and 82.3% in both structures. The histopathological findings revealed a chronic pyogranulomatous inflammatory process. To characterize the subgingival microbiota of the 14 sheep with periodontitis it was performed the Polymerase Chain Reaction (PCR), especially for microorganisms belonging to the red complex Socransky (Porphyromonas gingivalis, Tannerella forsythia and Treponema denticola) and other possible Gram-negative and Gram-positive periodontopathogens. There were identified in 50% of the samples Porphyromonas gingivalis, Tannerella forsythia in 92.8% and Treponema denticola in 78.5%. The three periodontal pathogens belonging to the red complex occurred simultaneously in 42.8% of the samples. We also identified in at least one of the examined animals Campylobacter rectus, Eikenella corrodens, Enterococcus faecium, Fusobacterium nucleatum, Prevotella intermedia, Prevotella nigrescens, and Prevotella loeschii. In the 14 samples Aggregatibacter actinomycetemcomitans, Dialister pneumosintes, Enterococcus faecalis and Porphyromonas gulae were not detected. The results showed that the sheep periodontitis occurred in the municipality of Benevides - PA has an infectious etiology and rushed a significant number of animals, many with bulging of the jaw; had high incidence in young and involved all of the adult animals post-mortem and after maceration examined.

No description available.

Yaws is a skin debilitating disease caused by Treponema pallidum subspecies pertenue with most cases reported in children. World Health Organization (WHO) aims at total eradication of this disease through mass treatment of suspected cases followed by an intensive follow-up program. However, effective diagnosis is pivotal in the successful implementation of this control program. Recombinase polymerase amplification (RPA), an isothermal nucleic acid amplification technique offers a wider range of differentiation of pathogens including those isolated from chronic skin ulcers with similar characteristics such as Haemophilus ducreyi (H. ducreyi). We have developed a RPA assay for the simultaneous detection of Treponema pallidum (T. pallidum) and H. ducreyi (TPHD-RPA). The assay demonstrated no cross-reaction with other pathogens and enable detection of T. pallidum and H. ducreyi within 15 min at 42 °C. The RPA assay was validated with 49 clinical samples from individuals confirmed to have yaws by serological tests. Comparing the developed assay with commercial multiplex real-time PCR, the assay demonstrated 94% and 95% sensitivity for T. pallidum and H. ducreyi, respectively and 100% specificity. This simple novel TPHD-RPA assay enables the rapid detection of both T. pallidum and H. ducreyi in yaws-like lesions. This test could support the yaws eradication efforts by ensuring reliable diagnosis, to enable monitoring of program success and planning of follow-up interventions at the community level.