Background. Security Information and Event Management (SIEM) systems today are sophisticated sets of software packages combined with hardware platforms, which can perform real-time analysis on security events and can respond to them before potential damage due to the actions of intruders. A huge number of systems rely on the continuous transmission of data through computer networks. Nowadays it is difficult to imagine a sphere of human activity that would not be affected by information technologies and would not use computer networks. Along with the means of protecting information, the technologies that are used by cybercriminals to achieve their goals are also improving. Moreover, the so-called insiders - information security perpetrators inside the protected perimeter, who can cause much more damage by their actions, as they are among the legitimate users and can have access to more confidential information - are becoming a growing threat. Objectives. To identify insider activity within an acceptable time inside the network, we need to develop a methodology to detect abnormal activity within the network using advanced data processing techniques, based on machine learning. After recreating the data processing system, we will also need to determine the most efficient algorithm that can be applied to the task of insider detection. Methods. The work analyzed research papers with similar objectives to investigate methods and technologies for securing against intruder intrusions, in conjunction with a study of machine learning techniques for detecting anomalies in the data. Experimental data were also collected containing information about network activity within the same network over two weeks. With this data, it is possible to conduct an experiment in network traffic processing using state-of-the-art technology. Results. During the study of relevant works, several effective ways to detect anomalies in the data were identified, technologies for processing large amounts of data using NoSQL were studied, and work on creating an experimental bench was performed. As a result, the experimental data obtained was sufficient to verify the effectiveness of the obtained solution. Conclusions. As a result, we analyzed existing approaches to detect insider activity within a computer system. Algorithms based on machine learning and big data processing methods were evaluated. In addition, a model for representing big data in NoSQL format was developed, which made it possible to create an architecture of a system for detecting insiders in computer networks using a graph database and machine learning methods.

Esta pesquisa examina os discursos, projetos e tentativas de elaboração e implantação de políticas culturais em duas universidades da América Latina – a Universidade de São Paulo, Brasil e a Universidade de Buenos Aires, Argentina. Estes documentos, obtidos por meio de pesquisa do campo, são registros organizados das práticas culturais utilizadas nestas duas instituições universitárias.A coleta desses registros oferece ainda a possibilidade da criação futura de um banco de dados sobre as práticas culturais na Universidade de São Paulo, Brasil. Durante o período de 1989/1995, no caso da Universidade de São Paulo, Brasil, houve uma primeira tentativa de direcionamento sistematizado de intenções e diretrizes para o desenvolvimento de projetos culturais. Neste período foi possível efetuar análises dos resultados de uma intervenção cultural que pretendia a construção de uma cena cultural antecipada na cidade de São Paulo. Por outro lado, na Universidade de Buenos Aires, após a intervenção ocorrida durante o extenso período do regime militar, 1976/1983, com a nova estrutura universitária, foi criado o Centro Cultural Reitor Ricardo Rojas, como tentativa de direcionamento na área cultural. Ao mesmo tempo, foi acampado o que ocorria socialmente no entorno da cidade de Buenos Aires. Assim, este estudo tem como objetivo comparar a administração cultural dessas duas universidades latino-americanas, verificando ao mesmo tempo os mecanismos utilizados na prática artística e o papel de cada um no cenário cultural dos respectivos países. Pretende-se alcançar esse objetivo por meio dos seguintes procedimentos: levantamento bibliográfico, cadastramento, análise e comparação das Ações Culturais postas em prática por essas instituições universitárias no período compreendido entre 1989 e 1995. / This research examines speeches, projects and attempts made by two Latin American universities: the University of Sao Paulo, Brazil, and the University of Buenos Aires, Argentina to elaborate and implement cultural policies. Documents obtained through field work, are in form of organized registers of cultural practices utilized in these institutions. The gathering of these registers will also facilitate the creation of a data banks of these cultural practices at the University of Sao Paulo,Brazil. In the case of the University of Sao Paulo, there was a first attempt made from 1989 through 1995, to systematize the channeling of intentions and instructions to the development of cultural projects. During this period it was possible to analyze results of cultural intervention, which intended the construct of an anticipated cultural scene in the city of Sao Paulo. On the other hand, at the end of the intervention of the military regime (1976-1983), the Centro Cultural Reitor Ricardo Rojas was created at the University of Buenos Aires, to channel cultural area, and document what was socially happening around the city of Buenos Aires. Thus, my objective is to compare the cultural administration of these two Latin American universities, observing the mechanisms used for an artistic practice and the role of each one of them on the cultural scene of these respective countries. This will be accomplished through the bibliographic gathering, to document, analyze, and compare cultural actions implemented by these institutions between 1989 and 1995.

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Esta pesquisa examina os discursos, projetos e tentativas de elaboração e implantação de políticas culturais em duas universidades da América Latina – a Universidade de São Paulo, Brasil e a Universidade de Buenos Aires, Argentina. Estes documentos, obtidos por meio de pesquisa do campo, são registros organizados das práticas culturais utilizadas nestas duas instituições universitárias.A coleta desses registros oferece ainda a possibilidade da criação futura de um banco de dados sobre as práticas culturais na Universidade de São Paulo, Brasil. Durante o período de 1989/1995, no caso da Universidade de São Paulo, Brasil, houve uma primeira tentativa de direcionamento sistematizado de intenções e diretrizes para o desenvolvimento de projetos culturais. Neste período foi possível efetuar análises dos resultados de uma intervenção cultural que pretendia a construção de uma cena cultural antecipada na cidade de São Paulo. Por outro lado, na Universidade de Buenos Aires, após a intervenção ocorrida durante o extenso período do regime militar, 1976/1983, com a nova estrutura universitária, foi criado o Centro Cultural Reitor Ricardo Rojas, como tentativa de direcionamento na área cultural. Ao mesmo tempo, foi acampado o que ocorria socialmente no entorno da cidade de Buenos Aires. Assim, este estudo tem como objetivo comparar a administração cultural dessas duas universidades latino-americanas, verificando ao mesmo tempo os mecanismos utilizados na prática artística e o papel de cada um no cenário cultural dos respectivos países. Pretende-se alcançar esse objetivo por meio dos seguintes procedimentos: levantamento bibliográfico, cadastramento, análise e comparação das Ações Culturais postas em prática por essas instituições universitárias no período compreendido entre 1989 e 1995. / This research examines speeches, projects and attempts made by two Latin American universities: the University of Sao Paulo, Brazil, and the University of Buenos Aires, Argentina to elaborate and implement cultural policies. Documents obtained through field work, are in form of organized registers of cultural practices utilized in these institutions. The gathering of these registers will also facilitate the creation of a data banks of these cultural practices at the University of Sao Paulo,Brazil. In the case of the University of Sao Paulo, there was a first attempt made from 1989 through 1995, to systematize the channeling of intentions and instructions to the development of cultural projects. During this period it was possible to analyze results of cultural intervention, which intended the construct of an anticipated cultural scene in the city of Sao Paulo. On the other hand, at the end of the intervention of the military regime (1976-1983), the Centro Cultural Reitor Ricardo Rojas was created at the University of Buenos Aires, to channel cultural area, and document what was socially happening around the city of Buenos Aires. Thus, my objective is to compare the cultural administration of these two Latin American universities, observing the mechanisms used for an artistic practice and the role of each one of them on the cultural scene of these respective countries. This will be accomplished through the bibliographic gathering, to document, analyze, and compare cultural actions implemented by these institutions between 1989 and 1995.

Made available in DSpace on 2014-06-11T19:24:46Z (GMT). No. of bitstreams: 0 Previous issue date: 2005-08-25Bitstream added on 2014-06-13T20:52:31Z : No. of bitstreams: 1 pedro_mam_me_sjrp.pdf: 977007 bytes, checksum: 33a5c50b033be5da84fd3ee27ae2d684 (MD5) / Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) / Neste trabalho estudou-se a influência de pré-tratamentos químicos, com emulsões a base de óleo e lecitina de soja e carbonato de potássio, sobre a cinética de secagem da uva Rubi. A secagem foi conduzida em um secador de leito fixo, à temperatura fixa de 60ºC, velocidade de ar de 0,9 m/s e umidade relativa do ar de secagem do ambiente na faixa de 18 a 20 %. A umidade inicial das amostras de uva Rubi variou entre 81 a 84% (base seca) e o conteúdo de sólidos solúveis ficou em torno de 11o Brix. O objetivo deste trabalho foi estudar um pré-tratamento alternativo a base de lecitina e óleo de soja na cinética de secagem da uva Rubi, otimizando as concentrações dessas substâncias de modo a minimizar o tempo de secagem. Foram calculados os coeficientes de difusividade aparente a partir do ajuste dos dados experimentais de secagem pela solução analítica da 2a Lei de Fick para uma esfera. A desconsideração do encolhimento da fruta levou ao péssimo ajuste dos dados pela 2a Lei de Fick. As curvas de secagem foram ajustadas pelo modelo de Page. A avaliação deste modelo foi feita pelo cálculo do R2 (> 0,99) e do RMS (< 30%). Este ajuste representou satisfatoriamente os dados experimentais às curvas. A cinética de secagem foi avaliada pela análise estatística dos efeitos das condições do pré-tratamento químico - tempo de imersão da uva e concentração de lecitina, carbonato de potássio e óleo de soja nas emulsões - sobre o parâmetro K do modelo de Page. Observou-se que o tempo de imersão e suas interações com as concentrações dos componentes em estudo não foram significativos no nível de 5%. Quando todos os componentes são empregados nas máximas e mínimas concentrações testadas, mesmo para o mínimo tempo de imersão (3 minutos), há um aumento no valor do parâmetro K, o qual está diretamente ligado... Resumo completo, clicar acesso eletrônico abaixo) / The influence of pretreatments of emulsions of lecithin, soya oil and potassium carbonate on drying kinetic of grape Rubi was studied in this work. The drying was carried out in a fixed bed dryer, at 60ºC, air velocity of 0,9 m/s and relative humidity of drying air in range from 18 to 20%. The initial moisture varied from 81 to 84% and soluble solids content was 11º Brix. The objective was to study an alternative pretreatment with lecithin, soya oil and potassium carbonate, optimizing the concentration of these substances with the aim of decrease the drying time. The apparent diffusion coefficients were calculated by the adjustment of the experimental data of drying by the analytical solution of the second Fick’s Law for a spherical geometry. The fruit shrinkage wasn’t taken in consideration. These carried out the bad adjustment of data by the second Fick`s Law. The drying curves were adjustment by Page’s model. The evaluation was goodness fit was shown through the analysis of the coefficient of determination (R2 > 0,99) and RMS (< 30%). This adjustment was good. The drying kinetics was valued through the statistical analysis of chemical pretreatment effects – dipping time of grape and concentration of lecithin, potassium carbonate and soya oil – on the parameter k of Page´s model. It was checked that dipping time and their interactions with the concentrations of the components in study were not significant in the level of 5%. When all components are spent in maximum and minimum concentration tested, even for the minimum dipping time (3 minutes), there is an increase in value of parameter K, which is straight into to the drying rate and reduction of drying time. The equilibrium moisture used in the calculation of adimensional for drying curves was obtained by isotherms data of Rubi grape without... (Complete abstract click electronic access below)

我國籃球運動，在1994年成立中華職業籃球聯盟，在歷經6年的經營，於1999年正式宣布解散，使我國籃球運動自此回歸業餘。在中華職業籃球聯盟之後，由中華民國高級中等學校體育總會主辦之「HBL高中籃球聯賽」再次地在國內引起一陣風潮，籃球賽事又再度吸引球迷目光。而中華民國大專院校體育總會所主辦之UBA大專籃球聯賽無論是電視轉播、媒體報導甚至現場觀眾人數，皆無法與人氣鼎沸的高中籃球聯賽相提並論。為何兩者雖皆屬於體育類非營利組織，主管機關同樣為內政部，目的事業主管機關亦同樣為教育部及行政院體育委員會，亦分別有大型企業之贊助，所經營之籃球聯賽卻有如此之差異？本研究發現，高中體總對籃球聯賽之行銷，設有臨時任務編組，並累積多年行銷運作過程之經驗，轉化為智慧資本，並與行銷公司及贊助廠商定期召開會議，不致在行銷過程中產生問題。而大專體總與行銷公司及贊助廠商之合作過程，則缺乏充分溝通協調，導致行銷效果不彰。大專體總與高中體總在其行銷運作時同樣面臨困難，包括球員配合行銷活動問題、運動組織與行銷公司配合問題等問題。另外，主動積極檢視行銷公司執行行銷之成效。除行銷本身運作之困難，尚有其他外在因素會阻礙行銷計畫之進行。包括學生球員與社會球隊球員重疊之問題、少子化趨勢等，皆增加了行銷之困難度。

While a relatively limited number of known oncogenes underlie a large percentage of cancers, a variety of new genes have emerged as low-frequency cancer drivers. Each of these new oncogenes represents a frontier for targeted therapy. However, the discovery of low-frequency targetable oncogenic drivers is challenging. This study focuses on the poorly understood Tyrosine kinase non-receptor-1 (TNK1), which has been reported to have both oncogenic and tumor suppressive functions. TNK1 has been identified to promote cancer cells survival and promote chemoresistance in multiple independent studies. On the other hand, whole-body constitutive deletion of TNK1 in mice caused an increase in spontaneous carcinomas and lymphomas. All in all, with no known regulatory mechanism and substrates of TNK1, the precise biological role of TNK1 is still unclear. To understand how TNK1 is regulated, we employed a proteomic approach to identify TNK1 interactors. We found out that TNK1 interacts with the phospho-binding protein 14-3-3 and this interaction is mediated by a cluster of MARK-mediated phosphorylations within the proline-rich domain. 14-3-3 binding retains TNK1 in the cytosol and maintains TNK1 in an inactive state. Release of TNK1 from 14-3-3 binding drives TNK1 to a heavy membrane fraction, where it becomes highly active. One unique feature of TNK1 is an ubiquitin association domain (UBA) on its C-terminus. Our data suggest that the UBA domain of TNK1 binds to poly-ubiquitin chains in nondiscriminatory manner. Remarkably, point mutations within the UBA that disrupt ubiquitin binding abolish TNK1 activation and oncogenic signaling, suggesting, to our knowledge, a unique UBA-centric mechanism of tyrosine kinase regulation. Finally, we used a structure-guided approach to identify a small molecule inhibiting TNK1 with high potency and selectivity. Such compound, TP-5801, inhibits TNK1 dependent STAT3 phosphorylation. TP-5801 also prolongs the survival of mice injected via tail vein with TNK1-driven Ba/F3 cells and reduces tumor burden in a subcutaneous xenograft model. In conclusion, our data reveal a mechanism of TNK1 regulation that controls its oncogenic tyrosine kinase activity and a potential strategy for TNK1 inhibition.

We studied the usefulness of genetic fusion to TELSAM polymers as an effective protein crystallization strategy. We observed novel properties in crystals of two TELSAM-target protein fusions. TELSAM as a crystallization chaperone shows rapid crystallization when it's fused to target proteins and possibly with a greater propensity. Some TELSAM-target fusions crystallized more rapidly than the same target protein alone. TELSAM-target proteins can be crystallized at relatively low protein concentrations such as 0.1 mg/mL. TELSAM requires no TELSAM polymers touching one another in the crystal lattice in order to form well-diffracting crystals. This lack of crystal contacts has not been observed in previously reported TELSAM crystal structures. Flexible TELSAM-target protein linkers can allow target proteins to find productive binding modes against the TELSAM polymer. This study tested TELSAM linker lengths varying by the number of glycines, such as 2xGly, 4xGly, 6xGly, 8xGly, and 10xGly. Only TELSAM fused to UBA with 2 and 4 glycine linkers were crystalized. TELSAM polymers can adjust their helical rise to allow fused target proteins to make productive crystal contacts, and fusion to TELSAM polymers increases avidity to stabilize weak inter-target protein crystal contacts. In conclusion, we report features of TELSAM-target protein crystal structures and outline future work needed to validate TELSAM as a crystallization chaperone and define the best practices for its use.

Structural biology uncovers life's secrets by studying protein structures via techniques like X-ray crystallography. This knowledge drives advancements in protein engineering for the improvement of human lives. Yet, obtaining high-quality crystals in X-ray crystallography is challenging. To overcome this, we used Translocation ETS Leukemia protein Sterile Alpha Motif domain (TELSAM), a promising polymer-forming crystallization chaperone (PFCC), to enhance protein crystallization. Human thirty-eight-negative kinase-1 (TNK1), a key player in cancer progression, possess a ubiquitin association (UBA) domain that binds polyubiquitin and regulates TNK1 activity and stability. Although sequence analysis hints at an unconventional TNK1 UBA domain architecture, its molecular structure lacks experimental validation. To gain insight into TNK1 regulation, we fused the UBA domain to the 1TEL crystallization chaperone and obtained crystals diffracting as far as 1.53 Ã…. 1TEL enabled solution of the X-ray phases. GG and GSGG linkers allowed the UBA to reproducibly find a productive binding mode against its 1TEL polymer and to crystallize at protein concentrations as low as 0.1 mg/mL. Our findings support a TELSAM fusion crystallization mechanism, highlighting fewer crystal contacts compared to traditional crystals. Both modeling and experimental validation indicate that the UBA domain exhibits selectivity towards polyubiquitin chain length and linkages. Radical S-adenosylmethionine (SAM) enzymes catalyze various radical-mediated substrate transformations. Despite the growing interest of computational enzyme design in industrial small molecule synthesis, radical SAM enzymes remain relatively unexplored. We used PyRosetta to leverage hydrogen bonding design (hbDes) and hydrophobic interaction design (hpDes) to enable a radical cyclization reaction on our selected substrate. Although the purified enzymes demonstrated activation potential with a reducing agent, enzymatic assays failed to exhibit activity against the reactant. To obtain successful results, addressing additional questions and issues is required, which may involve the implementation of machine learning.

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