MGS / Institute for Gender and Youth Studies / Extensive resources and information are available on HIV/AIDS, but there remain disparities between what is known and actual behavior, particularly among students at tertiary institutions. A lot has been done to create HIV awareness and prevent further infections among male and female students in Higher Education Institutions of South Africa. Despite the availability of information, awareness education and campaigns, free condoms and treatment, the HIV infections continue to increase in South Africa especially amongst the youth. The aim of the study was to investigate the knowledge, attitudes, and perceptions of male and female students at the University of Venda towards the “ABC” strategy for HIV prevention, and how these consequently influence their sexual behavior. Data was obtained from 32 students using a semistructured questionnaire. A non-probability, haphazard sampling technique was used to randomly select the participants. Descriptive statistics and thematic analysis were used for data analysis. The study findings contend that knowledge of HIV and its prevention measures always translates to positive sexual behaviour, as university students were found to be knowledgeable enough, but their attitudes and sexual behaviour were found not to be consistent enough. The researcher recommends future researchers to focus on the factors that hinder sexual abstinence, fidelity in sexual relationships, and condom use amongst Higher Education students, towards creating safer environments for them.