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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
301

Analysis of endo-polygalacturonase activity in a recombinant yeast containing a reconstituted PGU1 gene

Van Wyk, Herine 03 1900 (has links)
Thesis (MSc (Wine Biotechnology))--University of Stellenbosch, 2009. / The PGU1 gene encodes an endo-polygalacturonase, an enzyme that degrades pectin. Although the presence and function of this gene is well characterized in Saccharomyces cerevisiae, its regulation is very complex and not yet fully understood. Yeast producing a highly active polygalacturonase (PG) during alcoholic fermentation could potentially improve filtration and turbidity and also enhance extraction of certain aroma compounds. This could replace the addition of expensive commercial enzyme preparations that often contain unwanted enzymes. The first objective of this study was to evaluate PGU1 expression in recombinant strains of S. cerevisiae that originally lacked the PGU1 gene. A functional PGU1 gene and its promoter were successfully re-introduced into their native position in the genomes of five wine strains. Three of these strains recovered PG activity while two did not transcribe the gene and subsequently lacked activity. The three strains that recovered activity were used in microvinification experiments to determine the effect of PG-producing yeast on the aroma profile of the wine. No significant differences were observed in the volatile compounds production between the recombinants and their respective wild types, but some tendencies arose, especially for the monoterpene geraniol. The second objective of this study was to analyze the PGU1 gene and promoter from Saccharomyces paradoxus RO88 (a strain that exhibits high PG activity) and to compare it to those of S. cerevisiae S288C in order to identify differences that could potentially be responsible for the difference in their PG activities. Comparison of the gene sequences revealed several amino acid differences, one of which was in the peptide secretion signal. Analyses of the promoters also indicated some potentially important differences. Furthermore, S. cerevisiae strain VIN13, RO88 as well as two interspecies hybrids (all displaying varying PG activities) were compared under winemaking conditions. Clear differences were observed for the production of certain compounds. RO88 and the hybrids produced higher concentrations of certain volatile compounds, although they were not strong fermenters. Two recombinants, each containing a PGU1-overexpressing plasmid (one with the PGU1 gene from S. paradoxus and the other from S. cerevisiae), were also used in vinification to determine the effects of the different PGU1 gene on the aroma profile of the wine. Unfortunately, the plasmids were unstable and lost during the fermentation. Nevertheless, some tendencies were observed that indicated possible higher production of certain compounds by the recombinants compared to their wild types. This study identified that regulation of the PGU1 gene differs between strains with different genetic backgrounds. Certain differences were observed in the PGU1 gene and promoter sequences between S. cerevisiae and S. paradoxus that could potentially be the reason for the difference in their PG activities. From an oenological point of view, the presence of PGU1 in the genome of a fermenting strain tends to increase the aromatic potential of wine. These results provide a good platform for further studies on the PGU1 gene.
302

The root system of vines on a fertilization experiment with special reference to the phosphate status of the soil

Vink, J. De M. January 1955 (has links)
Thesis (MScAgric)--University of Stellenbosch, 1955. / No Abstract Available
303

n Vergelykende ampelografiese en ampelometriese studie van die tros van verskillende wyndruifcultivars

De Villiers, F. S. (Francois Stephanus) January 1987 (has links)
Thesis (MSc Agric.)--Stellenbosch University, 1987. / NO Abstract Available
304

Bioquímica para o curso de tecnologia em viticultura e enologia: um novo currículo / Biochemistry for Viticulture and Oenology Technology course: a new curriculum

Schoenmaker, Fernando 28 June 2019 (has links)
Atendendo a um mercado em crescimento nos últimos anos, o curso de Tecnólogo em Viticultura e Enologia é oferecido no Instituto Federal de São Paulo, campus São Roque para a formação de profissionais qualificados para o trabalho nas vinícolas e outros estabelecimentos comerciais voltados à produção e comercialização da uva, suco de uva e do vinho existentes, não só na região de São Roque-SP, como também em outras regiões do estado e do pais. O curso conta com a disciplina de Bioquímica, que vinha sendo aplicada em moldes convencionais. Na tentativa de melhorar a qualidade da disciplina, o interesse e o aproveitamento dos alunos, o presente projeto desenvolveu um novo currículo para a disciplina de Bioquímica, voltado para as especificidades e peculiaridades do curso de Viticultura e Enologia. Com assessoria de profissionais experientes, realizamos uma seleção criteriosa de conteúdos e definimos o grau de complexidade com que cada tema deveria ser trabalhado para atender às reais necessidades do Viticultor Enólogo. Os temas foram tratados de forma interdisciplinar e totalmente contextualizados, na tentativa de promover uma aprendizagem significativa. Sob a óptica do ensino centrado no aluno, procuramos incluir, ao longo da disciplina uma série de atividades que promovessem uma aprendizagem ativa, visando dividir com o aluno a responsabilidade pelo seu aprendizado. Utilizando o conceito de zona de desenvolvimento proximal de Vygotsky, inserimos atividades em grupo para estimular a interação e o desenvolvimento entre os pares.A coleta e análise de dados reuniu as abordagens quantitativa e qualitativa, utilizando questionários com escala Likert e entrevistas semiestruturadas. Nossos resultados mostram que o novo currículo foi bem aceito pelos estudantes. Apesar de ainda termos, em função do perfil de alunos que atendemos, um índice de insucesso considerado alto, a nova disciplina de Bioquímica foi capaz de motivar os estudantes e fornecer subsídios bioquímicos que contribuíram para o prosseguimento do curso. Desenvolvemos uma nova metodologia de seminários em grupo que contribuiu para o engajamento dos estudantes em relação aos conteúdos apresentados. Além dos conteúdos, a disciplina contribuiu para a formação científica dos alunos, forneceu um ambiente de aprendizagem colaborativa que estimulou o desenvolvimento da autonomia dos estudantes. Deste modo, o novo currículo proposto procurou promover uma contribuição mais efetiva da Bioquímica para a continuidade do curso e também para a atuação profissional dos futuros Viticultores Enólogos. / Taking into account a growing market in the recent years, the Viticulture and Oenology Technology course is offered at the Federal Institute of São Paulo, São Roque campus, to educate qualified professionals to work in wineries and other commercial establishments focused on production and commercialization of grape, grape juice and wine that are located not only in the region of São Roque-SP, but also in other regions of the state and the country. The course counts with the Biochemistry discipline, which had been applied in conventional formats. In an attempt to improve the quality of the discipline, as well as students interest and achievements, the present project developed a new curriculum for the discipline of Biochemistry, focused on the specificities and peculiarities of Viticulture and Oenology course. With the assistance of experienced professionals, we made a judicious selection of contents and defined the degree of complexity that each theme should be worked to meet the real needs of the Wine-grower Oenologist. The themes were treated in an interdisciplinary and fully contextualized way, in an attempt to promote significant learning. From the perspective of student-centered teaching, we seek to include throughout the course a series of activities that would promote an active learning, aiming to share with the student the responsibility for his/her learning. Using the concept of Vygotsky\'s Zone of Proximal Development, we included group activities to stimulate the interaction and the development among peers.Data collection and analysis gathered quantitative and qualitative approaches using Likert-scale questionnaires and semi-structured interviews. Our results show that the new curriculum has been well accepted by the students. In spite of the fact that we still have, due to the profile of students that we attend, a failure rate, which is considered high, the new discipline of Biochemistry was able to motivate the students and to provide biochemical subsidies that contributed to the follow up of the course. We developed a new methodology in the format of group seminars that contributed to the engagement of the students related to the presented contents. Besides the contents, the discipline contributed to the students\' scientific education, provided a collaborative learning environment, which stimulated the students\' autonomy development. In this way, the new proposed curriculum sought to promote a more effective contribution of Biochemistry to the continuity of the course and also to the professional performance of the future Wine-grower Oenologists.
305

Les paysages dans leur complexité : une ressource pour le développement territorial du piémont et des contreforts viticoles du Larzac (Languedoc-Roussillon) / Landscapes in their complexity : a resource for territorial development of the viticultural Piedmont and foothills from Larzac

Quiumento Velloso, Carolina 17 December 2013 (has links)
Avant de devenir objet de préoccupations politiques et d’aménagement de l’espace, le paysage a été sujet dans les plus différents et divers courants de la pensée scientifique. Aujourd’hui, le paysage occupe une place importante et croissante dans les politiques et les actions publiques, notamment dans le cadre des problématiques de développement durable àl’échelle des territoires. Il sort ainsi de la « connaissance scientifique » vers « l’agir dans le monde ». Cette thèse se propose d’étudier dans quelles mesures les paysages du piémont et des contreforts du Larzac – marqués par la vitiviniculture – peuvent être un élément moteur dans les processus de développement territorial durable. Avec l’appui de la théorie des ressources territoriales, cette thèse propose dans un premier temps d’éclairer comment le paysage devientressource. La conception et les dynamiques du paysage sont complexes et souvent difficilement saisissables par les acteurs du territoire, ce qui complique les processus de valorisation et d’activation de la ressource. Avec l’appui de la pensée systémique, ce travail de recherche propose donc une « nouvelle forme » de représenter les paysages dans leur complexité- le dessin riche. Ceci est un outil efficace pour les acteurs locaux dans les processus d’autoréflexion et d’autoapprentissage, qui permettent la définition de stratégies et d’actions qui activent la ressource pour promouvoir un développement territorial durable et le maintien des paysages non simplement « beaux », mais aussi « vivants », avec la participation et selon les regards des acteurs. / Before becoming subject of political and spatial development concerns, landscapes have been subject into the most different and various currents of scientific thought. At present, landscapes have an important and increasing role into politics and public actions, particularly in the sustainable territorial development. Landscapes therefore “leave” from the scientific knowledge to “act in the world”. They are then auspicious to be a resource to engender territorial development. The conception and dynamics of landscapes are though complex and often difficult to be understood (into their complexity) by the actors from the territory, which complicate the process of resource activation. The aim of this thesis is to study how the landscapes from the Larzac piedmont and the foothills – characterized by the vitiviniculture – can be a driving force into the process of (sustainable) territorial development. With support of systems thinking, this research also provides a "new method" to represent landscapes in their complexity. This representation is an efficient tool for local actors to self-reflection and self-learning processes that allows strategies definition and actions to activate the resource and then promote sustainable territorial development, as well as the maintenance of a “beautiful” but also “living” landscape, with actors’ visions and participation.
306

Caracteriza??o dos solos e avalia??o da aptid?o agr?cola das regi?es produtoras de vinhos finos de altitude de Santa Catarina / Soil characterization and evaluation of agricultural potential of the regions producing fine wines in Santa Catarina altitude

DORTZBACH, Denilson 02 June 2016 (has links)
Submitted by Jorge Silva (jorgelmsilva@ufrrj.br) on 2017-10-03T17:25:33Z No. of bitstreams: 1 2016 - Denilson Dortzbach.pdf: 2628494 bytes, checksum: 615986b2b9f4992ad6bc0756d35c6669 (MD5) / Made available in DSpace on 2017-10-03T17:25:33Z (GMT). No. of bitstreams: 1 2016 - Denilson Dortzbach.pdf: 2628494 bytes, checksum: 615986b2b9f4992ad6bc0756d35c6669 (MD5) Previous issue date: 2016-06-02 / CAPES / The soils of fine wines producing regions of Santa Catarina State, has particular altitude characteristics resulting from its lithology and high levels of organic matter. That differentiate the soil of the major producing regions of the world wine. The study was conducted in the regions of west and mountain through Santa Catarina, covering over 80 % of the properties where descriptions and collections of 38 modal profiles and vineyards spread over 45 farms were carried out. The objectives were: a) To characterize the distribution and interaction of soil formation factors and chemical weathering rates; b) evaluate and quantify the carbon, humic fractions in the profiles and ?13C in surface horizons of Cambisols; c) To characterize physical and chemically soils of the vineyards; d) To evaluate the agricultural potential through different methods, with subsequent adaptation of the System for Agricultural Assessment of land for the grape culture in addition to formulating a proposal for zoning for the wine industry of the state. The results indicate that pedogenetic soil was strongly influenced by the source material, weather and terrain. Cluster analysis separated by the similarity the soil profiles in three different groups. Predominate classes Cambisols and Nitossols. Humic substances allowed to discriminate the taxonomic orders of soil and showed ?13C in soil with the humic, little change in isotopic signature, and soils with moderate. The observed changes were derived from C3 and C4 plants crops. The vineyards have similar chemical properties, and thus can be grouped in a single region for a determination of Geographical Indication. As for agricultural suitability evaluating different methods indicated that the evaluated profiles have low agricultural aptitude for annual crops. Therefore, it was proposed an adaptation of the evaluation system of agricultural suitability of the land for the growing of vines, taking into account specific conditions of altitude in the SC state. Allied to this, the drafting of the zoning proposal is an important tool for the implementation of new vineyards. / Os solos das regi?es produtoras de vinhos finos de altitude do estado de Santa Catarina apresentam caracter?sticas particulares decorrentes da sua litologia e dos elevados teores de mat?ria org?nica, que os diferenciam dos solos das grandes regi?es produtoras de vinho do mundo. O estudo foi realizado nas regi?es do meio oeste e serrana de Santa Catarina, abrangendo mais de 80 % das propriedades, onde foram realizadas descri??es e coletas de 38 perfis modais e em vinhedos espalhados em 45 propriedades rurais. Os objetivos foram: a) caracterizar a distribui??o e a intera??o dos fatores de forma??o dos solos e a taxa de intemperismo qu?mico; b) avaliar e quantificar os teores de carbono, fra??es h?micas nos perfis e o ?13C em horizontes superficiais de Cambissolos; c) caracterizar f?sica e quimicamente os solos dos vinhedos; e d) avaliar a aptid?o agr?cola atrav?s dos diferentes m?todos, com posterior adapta??o do Sistema de Avalia??o Agr?cola das Terras, para a cultura da videira, al?m de formular proposta de zoneamento para a vitivinicultura do estado. Os resultados indicam que a pedog?nese dos solos foi fortemente influenciada pelos fatores material de origem, clima e relevo. A an?lise do agrupamento separou pela similaridade os perfis de solo em tr?s grupos distintos. Predominam as classes de Cambissolos e Nitossolos. As subst?ncias h?micas permitiram discriminar as ordens taxon?micas de solos e o ?13C revelando pouca altera??o na assinatura isot?pica nos solos com horizonte A h?mico. Nos solos com A moderado foram observadas altera??es decorrentes de mudan?as nas coberturas (plantas C3 ou C4). As ?reas dos vinhedos apresentam atributos qu?micos similares, podendo ser agrupadas em uma regi?o ?nica para eventual determina??o de Indica??o Geogr?fica. Quanto ? aptid?o agr?cola, ? avalia??o atrav?s de m?todos usuais na literatura resultou em classes que indicam baixa aptid?o para culturas anuais. Diante disto, foi proposta adapta??o do sistema de avalia??o da aptid?o agr?cola das terras, para a cultura da videira, considerando condi??es especificas de altitude no estado de SC e associada ? elabora??o de uma proposta de zoneamento para a implanta??o de novos vinhedos.
307

The reproductive biology of grapevines: factors that affect flowering and fruitset.

Longbottom, Mardi L. January 2007 (has links)
Molybdenum experiments: In Australia young Merlot vines sometimes suffer from vegetative disorders such as slow, zigzagged growth and leaf distortion. Merlot is also particularly known as a low- and inconsistent-yielding grape variety. Previous research showed that when foliar applications of molybdenum (Mo) were applied to Merlot vines the vegetative symptoms improved. More recently, when sodium molybdate was applied to Mo-deficient Merlot, yield improved; a function of increased bunch weight brought about by bigger berries. It has also been reported that at high concentrations, molybdenum might be detrimental to yield. Experiments were conducted on own-rooted Merlot (clone D3V14) vines in commercial vineyards in the Adelaide Hills (Hills) and at McLaren Vale, South Australia. Effects of molybdenum deficiency on the vegetative growth and yield of Vitis vinifera cv. Merlot: The aims of the current study were to: a) elucidate the mechanism by which molybdenum affects yield of Merlot; b) to monitor the effects of Mo-treatment on the balance between vine reproductive and vegetative growth; c) to monitor the residual effects of Mo-treatment on growth and yield of Merlot and; d) to determine whether high concentrations of molybdenum are detrimental to yield. Three rates of sodium molybdate were applied to vines in springtime (control = 0g, rate 1 = 0.101g and rate 2 = 0.202g sodium molybdate per vine). Vine molybdenum status was measured prior to treatment and again at flowering time using petiole, shoot tip and inflorescence analysis. The effects on vegetative growth were monitored at veraison, during dormancy and at budburst in the seasons following Mo-treatment. At flowering time, pollen vitality, pollen tube growth and flower structure were examined. Bunch number per vine, fruitset, berry weight and berry composition were measured at harvest. In the Hills, the controls had adequate molybdenum however, at McLaren Vale petiolar molybdenum concentration fell within the suggested deficiency range of 0.05-0.09 mg/kg in the petioles at flowering time. No visual symptoms of Mo-deficiency were observed on the experimental vines. At McLaren Vale, Mo-treatment reduced pruning weight and improved vine balance. Mo-treated vines in the Hills and at McLaren Vale were affected by delayed budburst in the season following Mo-treatment irrespective of their Mo-status. However, no seasonal carryover of molybdenum could be detected in tissue analysis at flowering time. Juice total soluble solids, pH and titratable acidity were not affected by Mo-treatment at McLaren Vale or in the Hills. However, juice from Mo-treated vines in the Hills had a significantly higher concentration of molybdenum than the controls. At McLaren Vale there was no significant difference in juice molybdenum concentration between treatments. In the Hills, yield was not affected by Mo-treatment. However, Mo-treated vines at McLaren Vale had significantly higher yields (approximately double) than the Mo-deficient controls. Bunch number per vine was not affected by Mo-treatment, either in the year that treatments were applied or in the following season. However, bunches from Mo-treated vines had significantly better fruitset resulting in more berries per bunch. Berry weight was affected by Mo-treatment in one season only. Yield was not detrimentally affected on vines that received the higher rate of sodium molybdate. In the Hills, Mo-treatment did not affect pollen numbers, pollen vitality or pollen tube growth. At McLaren Vale, where the controls were Mo-deficient, pollen vitality was not affected by Mo-treatment. However, pollen tube growth was significantly enhanced by Mo-treatment. Significantly more pollen tubes penetrated the ovules from Mo-treated vines and a higher proportion of ovaries had at least one penetrated ovule. Structural observations revealed that a significantly higher proportion of ovules from Mo-deficient vines were defective. The absence of an embryo sac in those ovules is probably the cause of pollen tube growth inhibition and subsequent poor fruitset. Effects of mode of pollination on yield of Merlot and the interacting effects of sodium molybdate sprays: Pollination experiments were conducted on field-grown own-rooted Merlot (clone D3V14) vines in commercial vineyards in the Adelaide Hills and at McLaren Vale in 2003-04 and in 2004-05. Inflorescences were supplied with supplementary Merlot pollen (self-pollination), with pollen from another variety (cross-pollination) or they were left to pollinate naturally (open pollination). In the Hills, mode of pollination did not affect fruitset or berry weight. In 2003-04 fruitset increased significantly at McLaren Vale when inflorescences were cross-pollinated with Semillon. Applying supplementary Merlot pollen also tended to improve fruitset, however none of the treatments affected berry weight. In 2004-05 there was no significant difference between treatments. These results indicate that Merlot may be a poor producer of pollen and may suffer from self-incompatibility. Given the significant improvements in yield gained by spring foliar applications of sodium molybdate to Mo-deficient Merlot vines, in 2005-06 a reciprocal experiment was conducted to separate the effects of Mo-treatment and mode of pollination on the male and female flower parts. The aims of this experiment were to: a) determine whether the male or female reproductive organs are more important in determining the success of fruitset of Merlot and; b) determine which remedial measure, Mo-treatment or pollination, is more effective at overcoming poor fruitset. Supplementary pollination treatments—cross-pollination (Semillon); self-pollination (Mo-deficient pollen); self-pollination (Mo-treated pollen) and; open-pollination—were applied to Mo-treated and Mo-deficient vines. Cross-pollinating Mo-deficient vines with Semillon significantly improved fruitset of Merlot compared to other pollination treatments on those vines, however applying molybdenum to the vines in springtime was more effective at improving fruitset. Within the Mo-treated vines the effects of supplementary pollination on fruitset were not thought to be of any practical significance. The results of this experiment provide further evidence that Mo-deficiency affects the female flower parts more than the male reproductive organs of Merlot. The occurrence of ‘star’ flowers in Australia: In 2003 faulty flowers were discovered on Canada Muscat grown in the Coombe Vineyard at the University of Adelaide’s Waite Campus. The Canada Muscat flowers opened from the top in ‘star’ formation in contrast to normal grape flowers, which shed the calyptra from its base. Star flowers were reported in French literature in the late 1800s. They were reported to as a symptom of a ‘disease’ that caused ‘coulure’, the cure for which was vine removal. The current report is the first known report of star flowers occurring in Australia. Through dissemination of the news of this discovery, several star flower variants were found in other varieties in South Australia. The association of star flowers with poor berry development and the frequency of the occurrence of star flowers suggest that this flower aberration may be affecting yield to a greater extent than previously recognised. This study provides a detailed description of two types of star flowers: those that occur in response to environmental conditions and those that occur every season. Other star flower variants are also documented. / http://proxy.library.adelaide.edu.au/login?url= http://library.adelaide.edu.au/cgi-bin/Pwebrecon.cgi?BBID=1280856 / Thesis (Ph.D.) -- University of Adelaide, School of Agriculture, Food and Wine, 2007
308

Turning Water into Wine: The Political Economy of the Environment in Southern California's Wine Country

Simms, Jason 01 January 2013 (has links)
This dissertation examines questions of water sustainability in contexts of wine production and state-led neoliberal development in the Temecula Valley, southern California, where wine tourism is at present being harnessed as an engine of economic growth. Natural and anthropogenic forces, such as global climate change, desertification, urban development, and the marketization and commodification of natural resources, affect the distribution and availability of water throughout the globe. As a result, the use of water, and associated political and environmental processes and consequences, in the production of global commodities, including wheat, citrus, and coffee, recently have come under increased scrutiny. Given wine's importance as a global commodity, and the concurrent growth of wine tourism as a worldwide phenomenon, local and regional water systems experience increasing strain to meet heightened demand for wine and the associated influx of tourists. This dissertation presents an ethnographic account of water use in the production of wine in Temecula, a desert-like setting already deficient in water that faces increasing human-induced pressures on its limited supply. Despite its social importance, very few dedicated ethnographies of wine and winemaking within the United States exist. This dissertation also describes the waterworld of Temecula, using (and critiquing) the model presented by Ben Orlove and Steven C. Caton that examines water in terms of value, equity, governance, politics, and knowledge systems, showing how these elements manifest in three "sites": the watershed, the water regime, and the waterscape. In Temecula, the winery serves as a central locus within the waterworld, a contested representation of the interests, goals, and perspectives of primary actors and stakeholders, while also serving as an important vector of landscape transformation through time. Despite this, no anthropological treatment examining water and winemaking within broader frameworks of the political economy of the environment and historical ecology is extant, a lacuna that this dissertation addresses. Throughout 2012, I conducted ethnographic fieldwork including archival research, interviews, and participant-observation. For the majority of my fieldwork, I spent time at an established winery in Temecula, during which I participated in many tasks related to wine production, with a focus on water use. Throughout this process, I interviewed dozens of people, including long-time residents, early pioneers in the Temecula wine industry, winery and vineyard employees, water management professionals at local and state levels, environmental service technicians, and many others. This dissertation demonstrates that under conditions of neoliberal development in challenging economic times in Temecula, environmental concerns such as water availability and sustainability are suppressed or downplayed in order to prioritize goals related to economic growth and development. Ultimately I suggest that developers and local business leaders are guiding this political legerdemain, even if only implicitly, above the din of objections from at least a good number of area wineries, vineyards, and residents. Also, I suggest that as an applied outcome, the totality of potential costs and outcomes at all scales, including regional, must be considered, rather than obfuscated, simplified, or restricted to a local boundary, especially in terms of natural resources and their governance, when such areas lie within locales inexorably connected within a delicate ecological web.
309

Grape Expectations

Vice President Research, Office of the January 2008 (has links)
Deciphering grape genomics and the yeast fermentation process is giving Hennie van Vuuren and Steven Lund clues on how to help wineries improve wine quality.
310

Le terroir, essai d'une réflexion géographique à travers la viticulture

Rouvellac, Éric 04 December 2013 (has links) (PDF)
Depuis les débuts de nos recherches nous travaillons sur le concept de terroir, sur un essai d'une réflexion géographique à travers la viticulture. Voilà pourquoi nous nous sommes intéressés à l'évolution de ce concept, à la fois dans les domaines de la recherche concernés et dans notre parcours de géographe, pour essayer de cerner un concept bicéphale entre nature et culture. Nous avons insisté à propos de la confusion qui est souvent entretenue avec le sol ou le substrat dans l'application du concept à la viticulture. Pour nous, le terroir doit être assumé sous tous les angles, et nous avons étudié comment deux approches, deux écoles s'ignorent aux sujet des terroirs viticoles alors que nous penchons pour une synthèse, une approche intégré du concept pour mieux le cerner et mieux modéliser le réel pour répondre aux demandes des viticulteurs. La justification du terroir arrive parfois à postériori, dans une logique déterministe ou alors de communication sans garde fou. Nous envisageons le terroir comme possibilité, comme potentiel, comme un concept applicable, de l'agro terroir jusqu'au marketing visant le consommateur. Nous avons approché le concept d'abord par des biais environnementaux avec une étude et une cartographie à grande échelle appliquées à deux grands vignobles à l'échelle du Sud-Ouest viticole, Cahors et Bergerac. Cartographier des terroirs nous a mené à vérifier par l'expérience la corrélation de ceux-ci avec la qualité du vin. Nous avons alors confronté notre vision du terroir viticole au Nouveau Monde avec l'Afrique du Sud. Nous avons analysé un système d'appellations viticoles complexe et sous-utilisé avec deux centres historiques, patrimoniaux, qui concourent à l'image des vins sud africains et un poids important de la coopération dans la diffusion de la vitiviniculture et l'amélioration de la qualité. Le terroir est mobilisé en Afrique du Sud de l'agro-terroir jusqu'à la représentation et l'instrumentalisation, notamment dans des tentatives de " déracialisation " du vin face aux défis post apartheid. Ces stratégies différenciées de marketings allant jusqu'à la fabrique des terroirs nous ont amenés à prolonger nos réflexions encore plus pour comprendre comment passait-on encore plus du cultural au culturel, à travers un vignoble artificialisé à haute dose, celui de Banyuls Collioure dans les Pyrénées orientales françaises. Il s'est d'abord agi d'analyser les enjeux autour d'un territoire patrimonialisé, partagé (écartelé ?) entre activités balnéaires, viticulture et paysages emblématiques. Qu'était-ce qu'un terroir viticole dans le contexte de Banyuls ? Nous sommes passés alors du terroir au paysage pour comprendre leur utilisation et leur évolution depuis le début du XIXème siècle jusqu'à nos jours à travers quatre exemples de bassin-versant. Fort de savoir comment avaient évolué les paysages viticoles, nous avons pu étudier comment la communication et le marketing frappaient l'imaginaire en inventant, en rêvant l'histoire du vignoble. Parfois classique, restant dans des domaines très historiques ou agro géologiques, la communication à propos des terroirs en arrive jusqu'à dissoudre le concept dans la poussée actuelle de l'architecture iconoclaste de certains domaines à travers le monde viticole.

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