Thesis (MScAgric (Viticulture and Oenology))--University of Stellenbosch, 2007. / Biogenic amines are nitrogenous compounds of low molecular weight found in most fermented foods, including wine. These biologically produced amines are essential at low concentrations for normal metabolic and physiological functions in animals, plants and micro-organisms. However, biogenic amines can have adverse effects at high concentrations and pose a health risk for sensitive individuals. Symptoms include nausea, hot flushes, headaches, red rashes, respiratory distress and fluctuations in blood pressure. A number of countries have implemented upper limits for histamine in food and wine. This development has already started to threaten commercial export transactions and may become more serious in the near future, especially in the competitive wine industry of today. The most important biogenic amines in wine include histamine, tyramine, putrescine, cadaverine and phenylethylamine which are produced from the amino acids histidine, tyrosine, ornithine, lysine and phenylalanine respectively. Biogenic amines are mainly produced in wine by microbial decarboxylation of the corresponding precursor amino acid. It may be produced by yeast during alcoholic fermentation, by lactic acid bacteria during malolactic fermentation, or potentially by spoilage microbes such as acetic acid bacteria and Brettanomyces. However, lactic acid bacteria are widely accepted as the main causative agents. Inoculation with commercial malolactic fermentation starter cultures that do not possess the relevant decarboxylase genes may inhibit the growth and activity of decarboxylase positive indigenous bacteria and as such control the production of biogenic amines in wine. In this study it was shown that co-inoculation of malolactic starter cultures together with alcoholic fermentation could reduce the incidence of biogenic amines in wine compared to conventional inoculation protocols; presumably because undesirable activities were restrained at an earlier stage during co-inoculation. It was also indicated in this work that in some cases the effect of co-inoculation on biogenic amine reduction may only be visible after a period of ageing. The frequency of biogenic amine occurrence in wines aged for a short period was generally higher in the presence of fermentation lees than in its absence. This work also included a preliminary investigation into the contribution of commercial wine yeast starter cultures to biogenic amine production. Diamines and polyamines (putrescine, spermidine and cadaverine) were produced to variable extents by all yeasts with very little or no production of physiologically important biogenic amines (histamine, tyramine and phenylethylamine). Another objective of this study was to evaluate the influence of common winemaking practices on biogenic amine production under winemaking conditions. We have shown that biogenic amine production by lactic acid bacteria could be influenced, amongst others, by the presence of precursor amino acids in the grape must or wine, the time of contact between juice or wine and grape skins, the time of contact between wine and yeast lees, the presence of microbial nutrients, wine pH, sulphite and ethanol levels, the phenolic composition of the wine and the number of decarboxylase positive lactic acid bacteria present in the wine. Lately, the wine industry is under increasing pressure to increase measures to ensure food safety and security and to eliminate any compound, present even in trace amounts that could reduce the wholesomeness of the wine. The need arises for a rapid and inexpensive method for quality control. In this study we investigated the potential to use Fourier transform infrared spectroscopy to rapidly screen for the presence of elevated levels of biogenic amines. This presents a novel method for the detection and quantification of total biogenic amines in wines.

Thesis (MScAgric (Viticulture and Oenology. Wine Biotechnology))--University of Stellenbosch, 2010. / ENGLISH ABSTRACT: Necrotrophic fungi infect many economically important crop plants. This results in great losses in the agricultural sector world-wide. Understanding the nature by which plants respond to pathogens is imperative for genetically enhancing disease resistance in plants. Research tools have significantly contributed to our understanding of how the plant responds to pathogen attack, identifying an array of defence mechanisms used by plants upon attack. Many fungal pathogens secrete endopolygalacturonases (endoPGs) when infecting plants. These hydrolytic enzymes are inhibited by polygalacturonase-inhibiting proteins (PGIPs) associated with plant cell walls. PGIPs are well characterised and their current known functions are all linked to endoPG inhibition and the subsequent upregulation of plant defence pathways. Work on grapevine PGIPs have shown that apart from being efficient antifungal proteins, leading to protection of the plant against Botrytis cinerea when overexpressed, PGIPs might also have additional functions linked to cell wall strengthening. This working hypothesis formed the motivation of this study where a cinnamyl alcohol dehydrogenase (CAD) (1.1.1.195) gene was targeted for functional analysis in tobacco (Nicotiana tabacum). Some previous work and genetic resources obtained is relevant to this study, specifically previously characterized transgenic tobacco lines overexpressing the Vitis vinifera pgip1 (Vvpgip1) gene. These lines have confirmed PGIP-specific resistance phenotypes against B. cinerea, as well as increased levels of CAD transcripts in healthy plants. Moreover, preliminary evaluations indicated increased lignin levels as well as differential expression of several other cell wall genes in these overexpressing lines (in the absence of infections). In this study we generated a transgenic tobacco population, overexpressing the native CAD14 gene, via Agrobacterium transformations. The transgene was overexpressed with the Cauliflower Mosaic Virus promoter (CaMV 35Sp). The CAD transgenic population was analyzed for transgene integration and expression and showed active transcription, even from leaves that normally don’t express CAD to high levels. These lines, together with the untransformed control, and a representative transgenic VvPGIP1 tobacco line previously characterized with elevated expression of CAD were used for all further analyses, specifically CAD activity assays of stems and leaves, as well as whole plant infections with B. cinerea. CAD enzyme activity assays were performed on healthy uninfected plant lines, without inducing native CAD expression or resistance phenotypes (i.e. without Botrytis infection). CAD activity was detected in leaves and stems, but a statistically sound separation between the CAD population and the untransformed control was only observed in the stems. The CAD assays also confirmed previous results that indicated that CAD transcription was upregulated in the PGIP line in the absence of infection. Overall, in all plant lines the stems exhibited 10-fold higher levels of CAD activity than the leaves, but the transgenic VvPGIP1 line showed a further 2-3-fold increase in CAD activity in the stems, when compared to the untransformed control and the majority of the CAD overexpressing lines. Disease assessment by whole plant infections with B. cinerea of the CAD transgenic plants revealed reduced disease susceptibility towards this pathogen. A reduction in disease susceptibility of 20 – 40% (based on lesion sizes) was observed for a homologous group of transgenic lines that was statistically clearly separated from the untransformed control plants following infection with Botrytis over an 11-day-period. The VvPGIP1 transgenic line displayed the strongest resistance phenotype, with reduction in susceptibility of 47%. The reduction in plant tissue maceration and lesion expansion was most pronounced in the VvPGIP1 line compared to the CAD transgenic plants, while the CAD transgenic plants showed more reduction than the untransformed control. In combination, the data confirms that CAD upregulation could lead to resistance phenotypes. Relating this data back to the previously observed upregulation of CAD in the VvPGIP1-overexpressing lines, the findings from this study corroborates that increased CAD activity contributes to the observed resistance phenotypes, possibility by strengthening the cell wall. In conclusion, this study yielded a characterized transgenic population overexpressing the CAD14 gene; this overexpression contributed to increased RNA transcription compared to the untransformed control plant, increased CAD activity (most notably in the stems) and a disease resistance phenotype against Botrytis. These findings corroborates the current working hypothesis in our group that PGIPs might have a role in preparing the plant cell for attack by contributing to specific cell wall changes. The exact mechanisms are still currently unknown and under investigation. The transgenic lines generated in this study will be invaluable in the subsequent analyses where these various phenotypes will be subjected to profiling and accurate cell wall analyses. / AFRIKAANSE OPSOMMING: Nekrotrofiese swamme infekteer en beskadig verskeie ekonomies belangrike gewasse. Dit lei wêreldwyd tot groot verliese vir die landbousektor. Dit is noodsaaklik om te verstaan hoe plante reageer teenoor patogene, sodat die siekteweerstand van plante verbeter kan word. Navorsingshulpbronne het beduidend bygedra tot die kennis van plantreaksies tydens patogeniese aanvalle, en het sodoende ‘n reeks van weerstandmeganismes, wat die plant inspan tydens ‘n aanval, geïdentifiseer. Verskeie patogeniese swamme skei endopoligalakturonases (endoPGs) af tydens plantinfeksie. Hierdie hidrolitiese ensieme word geïnhibeer deur poligalakturonase-inhiberende proteïene (PGIPs) wat met die plantselwand geassosieerd is. PGIPs is goed gekarakteriseerd en al hulle huidiglik bekende funksies is gekoppel aan endoPG inhibisie en die daaropvolgende opregulering van plant weerstandspaaie. Navorsing op wingerd PGIPs het gewys dat, afgesien van die feit dat PGIPs goeie antifungiese proteïene is wat lei tot beskerming van die plant teen Botrytis cinerea wanneer dit ooruitgedruk word, PGIPs ook moontlik addisionele funksies verrig wat verwant is aan selwandversterking. Hierdie werkshipotese vorm die motivering vir hierdie studie waarin ‘n sinnamiel alkohol dehidrogenase (SAD) (1.1.1.195) geen geteiken is vir funksionele analise in tabak (Nicotiana tabacum). Vorige navorsing en genetiese hulpbronne daardeur verkry is belangrik vir hierdie studie, spesifiek die gekarakteriseerde transgeniese tabaklyne wat die Vitis vinifera pgip1 (Vvpgip1) geen ooruitdruk. Hierdie lyne besit bevestigde PGIP-spesifieke weerstandsfenotipes teen B. cinerea, sowel as hoër vlakke van SAD transkripte in gesonde plante. Voorlopige analises het ook aangedui dat hierdie ooruitdrukkende lyne hoër lignien vlakke het, asook differensiële uitdrukking van verskeie ander selwandgene (in die afwesigheid van infeksie). In hierdie studie is ‘n transgeniese tabakpopulasie gegenereer wat die natuurlike tabak SAD14 geen ooruitdruk, deur middel van Agrobacterium transformasie. Die transgeen is ooruitgedruk deur die Blomkool Mosaïek Virus promoter (CaMV 35Sp). Die SAD transgeniese populasie is geanaliseer vir transgeen integrasie en uitdrukking en het aktiewe transkriptering getoon, selfs in blare waar daar normaalweg nie hoë vlakke van SAD uitgedruk word nie. Hierdie lyne, die ongetransformeerde wilde-tipe kontrole sowel as ’n verteenwoordigende transgeniese VvPGIP1 tabaklyn wat vooraf gekarakteriseerd was met hoë SAD uitdrukking, is gebruik vir alle verdere analises, spesifiek SAD aktiwiteitstoetse in stingels en blare, asook heelplantinfeksies met B. cinerea. Aktiwiteitsanalises van die SAD ensiem is gedoen op gesonde ongeinfekteerde plantlyne, sonder om natuurlike tabak SAD uitdrukking of weerstandsfenotipes te induseer (dus sonder Botrytis infeksie). SAD aktiwiteit is waargeneem in beide die blare en stingels, maar ‘n statisties betekenisvolle skeiding is slegs gevind tussen die SAD populasie en die ongetransformeerde kontrole in die stingels. Die SAD toetse het ook vorige resultate bevestig wat aangedui het dat SAD transkripsie opgereguleer word in die PGIP lyn in die afwesigheid van infeksie. Die stingels het oor die algemeen ‘n 10-voudige vermeerdering in SAD aktiwiteitsvlakke getoon in vergelyking met die blare, maar die transgeniese VvPGIP1 lyn het ‘n verdere 2-3-voudige verhoging in SAD aktiwiteit gehad in die stingels ,in vergelyking met die ongetransformeerde kontrole en die meerderheid van die SADooruitdrukkende lyne. Siekteweerstand ondersoeke deur middel van heelplantinfeksies met B. cinerea van die SAD transgeniese plante, het verminderde vatbaarheid aangedui ten opsigte van hierdie patogeen. ‘n Afname in siekte-vatbaarheid van 20 – 40% (gebaseer op wondgroottes) is waargeneem vir ‘n homoloë groep transgeniese lyne wat statisties betekenisvol geskei kon word van die ongetransformeerde kontrole plante na infeksie met Botrytis in ‘n infeksietoets wat 11 dae geduur het. Die VvPGIP1 transgeniese lyn het die mees weerstandbiedende fenotipe gehad, met ‘n afname in siekte-vatbaarheid van 47%. Die afname in plantweefselafbreking en wondgrootte was die duidelikste in die VvPGIP1 lyn in vergelyking met die SAD transgeniese plante, terwyl die SAD transgeniese plante ‘n groter afname aangedui het as die ongetransformeerde kontrole. In kombinasie het die data bevestig dat SAD opregulasie kan lei tot weerstandbiedende fenotipes. Hierdie data, in vergelyking met die vorige bevinding van opregulasie van SAD in die VvPGIP1-ooruitdrukkende lyne, bevestig dat hoër SAD aktiwiteit bydra tot die waargenome weerstandbiedende fenotipes, moontlik deur versterking van die plantselwand. Ter afsluiting, hierdie studie het ‘n gekarakteriseerde transgeniese populasie wat die SAD14 geen ooruitdruk gelewer; hierdie ooruitdrukking het bygedra tot hoër RNA transkripsie in vergelyking met die kontrole, verhoogde SAD aktiwiteit (veral in die stingels) en siekteweerstandbiedende fenotipes teenoor Botrytis. Hierdie bevindinge ondersteun die huidige werkshipotese in ons groep dat PGIPs moontlik ‘n rol speel in die voorbereiding van die plantsel teen infeksie deur spesifieke selwandveranderinge te veroorsaak. Die spesifieke meganismes is steeds onbekend en word verder ondersoek. Die transgeniese lyne wat tydens hierdie studie gegenereer is, sal baie belangrik wees in opvolgende analises waar hierdie verskillende fenotipes gebruik kan word om die profiel van selwandkomponente, maar ook die akkurate selwandsamestelling te bestudeer.

Thesis (MScAgric)--Stellenbosch University, 1932. / No Abstract Available

En Viticulture de Précision, l’imagerie multi-spectrale est principalement utilisée pour des dispositifs de télédétection. Ce manuscrit s’intéresse à son utilisation en proxidétection, pour la caractérisation du feuillage. Il présente un dispositif expérimental terrestre mobile composé d’un GPS, d’une caméra multi-spectrale acquérant des images visible et proche infrarouge, et d’un Greenseeker RT-100 mesurant l’indice Normalized Difference Vegetation Index (NDVI). Ce système observe le feuillage de la vigne dans le plan de palissage, en lumière naturelle. La parcelle étudiée comporte trois cépages (Pinot Noir, Chardonnay et Meunier) plantés en carré latin. En 2013, six jeux de données ont été acquis à différents stades phénologiques.Pour accéder aux propriétés spectrales de la végétation, il est nécessaire de calibrer les images en réflectance. Cela requiert l’utilisation d’une mire de MacBeth comme référence radiométrique. Lorsque la mire est cachée par les feuilles, les paramètres de calibration sont estimés par une interpolation linéaire en fonction des images les plus proches sur lesquelles la mire est visible. La cohérence de la méthode d’estimation employée est vérifiée par une validation croisée (LOOCV).La comparaison du NDVI fournie par le Greenseeker avec celui déterminé via les images corrigées permet de valider les données générées par le dispositif. La polyvalence du système est évaluée via les images où plusieurs indices de végétation sont déterminés. Ils permettant des suivis de croissance de la végétation originaux offrant des potentialités de phénotypage ou une caractérisation de l’état sanitaire de la végétation illustrant la polyvalence et le gain en précision de cette technique. / Mutispectral imaging systems are widely used in remote sensing for Precision Viticulture. In this work, this technique was applied in the proximal sensing context to characterize vine foliage. A mobile terrestrial experimental system is presented, composed of a GPS receiver, a multi-spectral camera acquiring visible and near infrared images, and a Greenseeker RT-100 which measures the Normalized Difference Vegetative Index (NDVI). This optical system observes vine foliage in the trellis plan, in natural sunlight. The experimental field is planted with Chardonnay, Pinot Noir and Meunier cultivars in a latin squared pattern. In 2013, six datasets were acquired at various phenological stages.Spectral properties of the vegetation are accessible on images when they are calibrated in reflectance. This step requires the use of a MacBeth colorchart as a radiometric reference. When the chart is hidden by leaves, the calibration parameters are estimated by simple linear interpolation using the results from resembling images, which have a visible chart. The performance of this method is verified with a cross-validation technique (LOOCV).To validate the data provided by the experimental system, the NDVI given by the Greenseeker was compared to those computed from the calibrated images. The assessment of the versatility of the system is done with the images where several indices were determined. It allows an innovative follow-up of the vegetative growth, and offering phenotyping applications. Moreover, the characterization of the sanitary state of the foliage prove that this technique is versatile and accurate.

Depuis une perspective théorique au croisement de la sociologie économique et de la sociologie de la connaissance, cette thèse a pour objectif d’examiner les mécanismes sociaux à l’œuvre au moment de la conversion vers l’agriculture biologique. Sur la base de l’étude empirique et de l’analyse d'un réseau complet qui révèle les interdépendances entre plus de 60 viticulteurs certifiés ou en cours de certification à l'agriculture biologique en Côte de Beaune, sont analysés les processus d’apprentissage collectifs sur lesquels s’appuie cette transition. Cette thèse montre l’existence de deux processus-Type d’apprentissage qui se définissent en fonction des frontières des groupes de référence (ou niches sociales) ; elle analyse le rôle des pionniers du milieu étudié comme pivot de l’action collective dans ces deux processus d’apprentissage, et explore enfin les régularités qui structurent les échanges au sein du milieu. Ces régularités permettent de constater que les viticulteurs intègrent dans leur raisonnement des aspects identitaires qui différencient les viticulteurs bio « de la première heure » des novices, au-Delà d'aspects économiques liés à une tendance à la « coopétition ». / This PhD dissertation combines the approaches of economic sociology and sociology of knowledge in order to analyze the social mechanisms underlying the conversion into organic farming. Based on an empirical study and analysis of a complete social network describing the interdependencies among over 60 wine producers certified in organic farming or in the process of getting the official certification in French Côte de Beaune, this research analyzes the collective learning process on which this transition relies. The thesis proves the existence of two types of learning process, which are defined by the social borders of reference groups (or social niches); it analyzes the key role of pioneers of the social milieu in both learning processes and studies the regularities structuring social exchanges. These regularities allow us to confirm that wine producers take into account in their reasoning both the identity aspect related to their conversion to organic farming and their economic position, in a trend towards “coopetition”.

La thèse porte sur la proposition d’un protocole pour le développement durable de la viticulture du Brésil, avec un approche territorial, a partir de deux études exploratoires au Brésil, dans les régions viticoles de la Campanha Gaúcha et de la Vale do São Francisco, et basée sur des investigations préliminaires, ce qui impliquait des pratique de terrain dans trois pays (la France, l’Italie et l’Espagne) et l’observation des standards et programmes dans autres cinq (l’Afrique du Sud, l’Australie, la Nouvelle Zélande, les États Unis et le Chili). Le protocole proposé s’appelle BaccuS, qui est structurée sous forme matricielle, avec un axe correspondant à des dimensions et d'autres à des lignes directrices, organisées dans une ordre croissant de complexité et de développement. Les cinq dimensions (environnemental, économique, social, politique-institutionnelle et territorial) et les quatre directrices (la gestion, l’articulation et coopération, l’innovation et l’apprentissage et le développement durable) sont articulés par dix-huit thèmes, qui représentent les aspects principaux pour l’action pour promouvoir le développement durable dans les territoires du vin. Chaque thème a des indicateurs, qui sont détaillés en facteurs à considérer et des exemple pratique d’application au Brésil ou dans le contexte international. Ainsi, dix indicateurs de synthèse indiquent des initiatives ou programmes que peuvent être démarrés et répercuter positivement dans d’autres indicateurs. Le BaccuS peut être utilisé dans des échelles diverses, dans les producteurs ou territoires. / The thesis involves the proposition of a framework for the sustainability of viticulture in Brazil using a territorial approach built from two exploratory studies in Brazilian wine regions of Campanha Gaúcha and Vale do São Francisco based on preliminary studies. Those studies involved technical visits in three countries (France, Italy and Spain) and frameworks’ observations in another five (South Africa, Australia, New Zealand, USA and Chile). The framework proposed is called BaccuS. It is structured on a matrix basis, with an axis corresponding to dimensions and other to guidelines, both in ascending order of complexity and development. There are eighteen topics that represent areas to act to promote the sustainability in the wine territories. They articulate the five sustainability dimensions (environment, economic, social, political-institutional and territorial) and the four guidelines (management, articulation and cooperation, innovation and learning and sustainability). Each topic has indicators, in which factors to consider and practical examples in Brazil or internationally are detailed. Additionally, ten synthesis indicators specify initiatives or programs that can be implemented to impact positively on several indicators. The BaccuS framework can be applied in several scales, in the winery or the territory. / A tese envolve a proposição de um protocolo para a sustentabilidade da vitivinicultura do Brasil com uma abordagem territorial a partir de dois estudos exploratórios na Campanha Gaúcha e no Vale do São Francisco baseados em pesquisas prévias. Essas pesquisas contaram com trabalho de campo em três países (França, Itália e Espanha) e observação de protocolo em outros cinco (África do Sul, Austrália, Nova Zelândia, Estados Unidos e Chile). O protocolo proposto é chamado BaccuS. É estruturado em forma matricial, com um eixo correspondendo a dimensões e outro a diretrizes, ambas em ordem crescente de complexidade e desenvolvimento. As cinco dimensões de sustentabilidade (ambiental, econômica, social, político-institucional e territorial) e as quatro diretrizes (gestão, articulação e cooperação, inovação e aprendizado e sustentabilidade) são articuladas por 18 temas, que representam áreas principais para a ação, de modo a promover a sustentabilidade no território do vinho. Cada tema possui indicadores, nos quais exemplos práticos de aplicação no Brasil ou internacionalmente são considerados. Além disso, dez indicadores de síntese indicam iniciativa ou programas que podem ser implantados e repercutir positivamente em diversos indicadores. O protocolo BaccuS pode ser aplicado em diversas escalas, na vinícola ou no território.

The topic of the diploma thesis is the design of a wine hotel for a vineyard Nikolsburg in Mikulov. Main idea of this project is to create a new hotel building combined with the production of wine so it would naturally fit the given landscape and so it wouldn’t disturb its natural character. This building was also designed so it has extraordinary views of the whole wine area of Mikulov, the Mikulov Chateau and the Holy Hill. Primary function of the building is accommodation in a 4-star hotel rooms and a clean production of wine, which includes maturing, bottling and labeling. There are other services added, such as a restaurant, a wine bar, tasting salon with immediate a wine cellar designed for wine maturing and also last but not least a wellness center with sauna world to make your stay more pleasant.The architectural concept is mainly based on the view axis and the shape of given landscape. Hinged façade made of timber cladding is inspired by the wine production and regularity of the vineyards.

This paper examines vineyard perceptions and adoption of climate change adaptation and water conservation measures in the Paso Robles American Viticultural Area (AVA). A survey was distributed to all 220 vineyards and vineyard management companies that operate in the AVA, with a 53.64% response rate. The objective of the survey was to determine vineyard manager and owner attitudes towards climate change and adaptation, as well as their perceptions of how these threats would impact their operation. A second objective was to document the current level of water conservation and climate adaptation while identifying the barriers and opportunities for further adoption of these practices. The third objective was to share a climate projection to assess perceived impacts, attitudes, and perceptions of the projection and capacity to adapt. The final objective was to develop a typology to assist targeted outreach of vineyards. Our results showed that the most important current climate impact is heat, with water regulations and supply being the biggest concern in the future. Vineyards were also found to be relatively unsure about their capability to further implement adaptation measures, with relatively low adoption of most practices already. Decision support tools, like the climate projection, were found to be useful and desired by vineyards for management. Results of our study showed that trust in the information source can be a barrier to use of these tools. We identified further barriers to adoption of practices and identified an outreach strategy using a typology of vineyards, which focused on smaller vineyards and those without wineries. These results can be used to increase efficacy of government and NGO programs that aim to support climate adaptation and water conservation in the region.

Thesis MSc(Agric)--Stellenbosch University, 1973. / ENGLISH ABSTRACT: no abstract available / AFRIKAANSE OPSOMMING: geen opsomming

Thesis (MScAgric)--Stellenbosch University, 2011. / ENGLISH ABSTRACT: Shiraz/110R grapevines, growing in a fine sandy loam soil in the Breede River Valley, were subjected to ten different drip irrigation strategies during the 2006/07, 2007/08 and 2008/09 seasons. Grapevines of the control treatment (T1) were irrigated at 30% to 40% plant available water (PAW) depletion throughout the growing season. Grapevines of three treatments were irrigated at 70% to 80% PAW depletion from bud break until véraison (i.e. when ca. 95% of grape berries have changed colour), followed by either irrigation at 30% to 40% PAW depletion (T2) or a continuous deficit irrigation (CDI) strategy (T3) or irrigation at 70% to 80% PAW depletion (T4) during ripening. The CDI strategy was obtained by applying ca. half the volume of water that was applied to the control. This allowed the soil to dry out gradually between physiological stages (i.e. bud break and véraison or véraison and harvest). Grapevines of three further treatments were irrigated at ca. 90% PAW depletion from bud break until véraison, followed by irrigation at 30% to 40% PAW depletion (T5) or a CDI strategy (T6) or irrigation at ca. 90% PAW depletion (T7) during ripening. Grapevines of two treatments were irrigated by means of a CDI strategy from bud break until véraison. For both treatments, the soil water content (SWC) was allowed to dry out gradually until ca. 90% PAW depletion was reached. After véraison, the SWC of the one treatment was maintained at ca. 90% PAW depletion by applying only four small irrigations of three hours each during ripening (T8). The soil of the other treatment, received an irrigation at véraison to refill the SWC to field capacity (T9) followed by the CDI strategy during ripening. Grapevines of the tenth treatment were irrigated at ca. 90% PAW depletion between bud break and véraison followed by a partial profile refill (PPR) strategy during ripening (T10). In order to obtain the PPR strategy, SWC was only maintained between 40% and 60% PAW depletion. Evapotranspiration varied between 3.5 mm/day and 0.1 mm/day for driest and wettest treatments, respectively, during the period between December and February. This was substantially less than the volumes required for full surface irrigation. For irrigations applied at 30% to 40% PAW depletion (T1), 70% to 80% PAW depletion (T4) and ca. 90% PAW depletion (T7) levels throughout the season, crop coefficients for the Penman-Monteith reference evapotranspiration (ETo) were 0.4, 0.2 and 0.1, respectively. Under the given conditions, the different irrigation strategies did not have any effect on root distribution and density. Shoot growth of grapevines exposed to high to severe water deficits in the pre-véraison period stopped before mid December. Shoots of grapevines that were exposed to high or severe water deficits before véraison followed by more frequent irrigation during ripening showed active re-growth. These trends occurred during all the seasons. The level of PAW depletion reflected strongly in the plant water potential in the grapevines. Leaf water potential was influenced by the prevailing atmospheric conditions, whereas stem water potential was less sensitive to atmospheric conditions, but responded more directly to soil water availability. Due to the good relationships between pre-dawn leaf, mid-day leaf, mid-day stem and total diurnal water potential, it was possible to re-classify the water status in terms of previous classifications for these water potentials based on pre-dawn measurements. Water constraints in T1, T2 and T5 grapevines were classed as experiencing no stress, whereas the T7 and T8 ones experienced strong to severe water constraints before harvest. High frequency irrigation strategies during ripening delayed sugar accumulation due to dilution of sugar in the larger berries. Except for the wettest strategy, and where grapevines were subjected to the CDI strategy throughout the season, berry mass increased during ripening, i.e. from véraison to harvest. Water deficits had a negative effect on berry mass, bunch size and yield. Where higher soil water depletion levels were allowed, irrigation strategies had a positive effect on the irrigation water productivity of grapevines compared to the frequently irrigated or CDI strategies. Higher water constraints in grapevines, particularly during ripening, improved sensorial wine colour and enhanced some of the more prominent wine aromas, e.g. spicy and berry. Grapevines that were irrigated at a high frequency during ripening produced wines with diluted character flavours and aromas and inferior overall quality. Under the given conditions, sensorial wine colour and spicy character were the dominant factors in determining overall sensorial wine quality. / AFRIKAANSE OPSOMMING: Shiraz/110R wingerdstokke in ‘n fyn sandleem grond in die Breede Rivier vallei is gedurende die 2006/07, 2007/08 en 2008/09 seisoene met tien verskillende drupbesproeiingstrategieë besproei. Wingerdstokke van die kontrole (B1) is deur die seisoen by 30% to 40% plant beskikbare water (PBW) onttrekking besproei. Drie behandelings is tussen bot en deurslaan (wanneer ca. 95% van die korrels verkleur het) by 70% tot 80% PBW onttrekking besproei, gevolg deur besproeiing by 30% tot 40% PBW onttrekking (B2), ‘n deurlopende tekort besproeiing (DTB) strategie (B3) of besproeiing by 70% tot 80% PBW onttrekking (B4) gedurende rypwording. In die geval van die DTB strategie is ongeveer die helfte van die volume water toegedien wat by die kontrole toegedien is. Laasgenoemde strategie het die grond toegelaat om geleidelik tussen fisiologiese fases (i.e. tussen bot en deurslaan of tussen deurslaan en oes) uit te droog. Drie ander behandelings is by ca. 90% PBW onttrekking tussen bot en deurslaan besproei, gevolg deur besproeiing by 30% tot 40% PBW onttrekking (B5) of ‘n DTB strategie (B6) of besproeiing by ca. 90% PBW onttrekking (B7) gedurende rypwording. Wingerdstokke van twee ander behandelings is d.m.v. ‘n DTB strategie vanaf bot tot deurslaan besproei. Beide behandelings se grondwaterinhoud (GWI) was toegelaat om geleidelik uit te droog tot ca. 90% PBW onttrekking bereik was. Na deurslaan was die GWI van die een behandeling naby ca. 90% PBW onttrekking gehandhaaf deur slegs vier klein besproeiings van drie uur elk gedurende rypwording toe te pas (B8). Die grond van die ander behandeling het tydens deurslaan ‘n besproeiing ontvang om die GWI tot by veldkapasiteit te hervul (B9) en is tydens rypwording weer d.m.v. ‘n DTB strategie besproei. Stokke van die tiende behandeling is tussen bot en deurslaan by ca. 90% PBW onttrekking besproei, gevolg deur besproeiing d.m.v. ‘n gedeeltelike profiel hervul (GPH) strategie tydens rypwording (B10). Om ‘n GPH strategie toe te kon pas, is tussen 40% en 60% PBW ontrekking gehandhaaf. Evapotranspirasie het tussen 3.5 mm/dag en 0.1 mm/dag vir onderskeidelik die natste en droogste behandelings tussen Desember en Februarie gevarieer. Dit was aansienlik laer as volumes wat vir voloppervlak besproeide wingerde benodig word. In die geval van besproeiing by 30% tot 40% PBW onttrekking (B1), 70% tot 80% PBW onttrekking (B4) en ca. 90% PBW onttrekking (B7) deur die loop van die seisoen was die gewasfaktore vir die verwysingverdamping (ETo) 0.4, 0.2 en 0.1 onderskeidelik. Onder die gegewe toestande het die verskillende besproeiingstrategië geen effek op die worteldigtheid en –verspreiding gehad nie. Lootgroei van wingerdstokke wat aan hoë tot baie hoë watertekorte blootgestel was voor deurslaan, het voor middel Desember gestop. Lote van wingerdstokke wat aan hoë tot baie hoë watertekorte voor deurslaan blootgestel is, gevolg deur besproeiing teen ’n hoë frekwensie tydens rypwording, het aktiewe hergroei getoon. Die PBW ontrekkingspeil het sterk in die plantwaterpotensiale van wingerdstokke weerspieël. Blaarwaterpotensiaal is deur heersende klimaatstoestande beïnvloed, terwyl stamwaterpotensiaal minder sensitief teenoor die klimaat was, maar meer direk deur die beskikbaarheid van grondwater beïnvloed is. Vanweë die goeie verband tussen voordagbreek blaar-, mid-dag blaar-, mid-dag stam- en totale daaglikse waterpotensiaal, was dit moontlik om water status van die stokke te her-klassifiseer in terme van vorige vir waterpotensiaalklassifikasies wat op voordagbreek waardes gebasseer is. Waterspanning in B1, B2 en B5 stokke is as geen spanning geklassifiseer, terwyl dié van B7 en B8 voor oes in die hoë tot baie hoë klasse geval het. Hoë frekwensie besproeiing strategieë gedurende rypwording kan suikertoename a.g.v. die groter korrels vertraag. Met die uitsondering van die natste strategie, asook waar stokke volgens die DTB strategie deur die seisoen besproei is, het korrelmassa gedurende rypwording toegeneem. Watertekorte het ’n negatiewe effek op korrelmassa, trosgrootte en produksie gehad. Besproeiingstrategieë waar ’n hoë mate van grondwateronttrekking voor besproeiings toegelaat is, het ’n positiewe effek op die besproeiingwaterproduktiwiteit van wingerd in vergelyking met gereelde besproeiings of ‘n DTB strategie gehad. Watertekorte, veral gedurende rypwording, het ’n verbetering in sensoriese wynkleur en meer prominente wyn aromas, tot gevolg gehad. Besproeiing teen hoë frekwensies gedurende rypwording, het wyne met ’n afgewaterde smaak en aroma karakters asook ’n swak algehele gehalte produseer. Sensoriese wynkleur en spesery karakter die dominante faktore in die bepaling van algehele kwaliteit.