The Vo sector of the vacuolar H+-ATPase is a multi-subunit complex that forms a proteolipid pore. The largest subunit in this complex is the a subunit which has four isoforms (a1-a4). The isoform(s) critical for secretory vesicle acidification has yet to be identified. Using a cell line derived from rat pheochromocytoma in which Voa1 and/or Voa2 had been down-regulated this study revealed that Voa1, and to a lesser extent, Voa2 are critical for acidifying dense-core vesicles (DCVs). The acidification defects resulting from down-regulation of Voa1 and Voa1/ Voa2 were suppressed by the expression of knockdown-resistant Voa1. Defects in DCV acidification resulted in reductions in their transmitter uptake and storage. Lastly, Ca2+-dependent peptide secretion appeared normal in Voa1 and Voa1/ Voa2 knockdown cells. . This study demonstrated that Voa1 and Voa2 cooperatively regulate dense-core vesicle acidification as well as transmitter uptake/storage, while they may not be critical for dense-core vesicle exocytosis.

The Vo sector of the vacuolar H+-ATPase is a multi-subunit complex that forms a proteolipid pore. The largest subunit in this complex is the a subunit which has four isoforms (a1-a4). The isoform(s) critical for secretory vesicle acidification has yet to be identified. Using a cell line derived from rat pheochromocytoma in which Voa1 and/or Voa2 had been down-regulated this study revealed that Voa1, and to a lesser extent, Voa2 are critical for acidifying dense-core vesicles (DCVs). The acidification defects resulting from down-regulation of Voa1 and Voa1/ Voa2 were suppressed by the expression of knockdown-resistant Voa1. Defects in DCV acidification resulted in reductions in their transmitter uptake and storage. Lastly, Ca2+-dependent peptide secretion appeared normal in Voa1 and Voa1/ Voa2 knockdown cells. . This study demonstrated that Voa1 and Voa2 cooperatively regulate dense-core vesicle acidification as well as transmitter uptake/storage, while they may not be critical for dense-core vesicle exocytosis.

This paper deals with the possibilities of designing the module for control of the attenuation of the optical signal. The paper describes basics of ber optic transmission and beam e ects. Variants of attenuation cells are characterized in this paper and based on the ndings a solution of the module for control of the attenuation of the optical signal is proposed. Practical section of the thesis deals with the design and physical construction of module for control of the attenuation of the optical signal using MEMS variable optical attenuator.

Submitted by JÚLIO HEBER SILVA (julioheber@yahoo.com.br) on 2017-12-20T11:07:44Z No. of bitstreams: 2 Dissertação - Jordana Albino Oliveira - 2017.pdf: 3150287 bytes, checksum: 3680510e53922e021ec129a2fd4b0796 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) / Approved for entry into archive by Luciana Ferreira (lucgeral@gmail.com) on 2017-12-21T12:33:29Z (GMT) No. of bitstreams: 2 Dissertação - Jordana Albino Oliveira - 2017.pdf: 3150287 bytes, checksum: 3680510e53922e021ec129a2fd4b0796 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) / Made available in DSpace on 2017-12-21T12:33:29Z (GMT). No. of bitstreams: 2 Dissertação - Jordana Albino Oliveira - 2017.pdf: 3150287 bytes, checksum: 3680510e53922e021ec129a2fd4b0796 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Previous issue date: 2017-06-28 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES / The research seeks to analyze the essayist thought, the gesture of montage and the production of memory on the film-essay Rocha que Voa (2002) by the brazilian filmmaker Eryk Rocha. Film of assembly of sonic and imaginary elements, symbiotic and amalgamated, that transit between temporalities and localities producing memory of the latin american cinema of the years of the 60s and 70s. Memory that is not a subject or a whole movement, but immemorial, absolute and involuntary as the contact of a self and a world. In the movement of looking at the past and dialoguing with it, the files that constitute the film are updated in the present. Archives of Havana, Glauber Rocha, Latin American cinema, and aesthetic and political dimensions tensioned and in relation to the cinematographic language and with the established settlements with the world. Through bibliographical research and a film analysis, we seek to understand how the traces of images (visual and sound) are mobilized by the experience of the filmmaker and performed by the montage At first, we seek to contextualize the film within the context of Brazilian contemporary documentary and to approach it with the concept of filmessay, conceiving the essaistic gesture as the guide of our process of film analysis and bibliographic research. Next, we discuss the appropriation of archives and the unrelated relations of images with memory and history. The poetic-constructive operation of the montage marked by the heterogeneity of materials and their new combinations gains prominence in the figure of the palimpsest that summons in form and gesture the aporia of a past that is not over but still, and continues to be updated in the present. / A pesquisa busca analisar o pensamento ensaístico, o gesto da operação da montagem e a produção de memória no filme-ensaio Rocha que Voa (2002), do cineasta brasileiro Eryk Rocha. Filme de montagem de elementos sonoros e imagéticos, simbióticos e amalgamados, que transitam entre temporalidades e localidades produzindo memória do cinema latino-americano dos anos das décadas de 60 e 70. Memória essa não apenas de um sujeito ou de um movimento todo, mas memória imemorial, absoluta e involuntária enquanto contato de um eu e de um mundo. No movimento de olhar o passado e com ele dialogar, os arquivos que constituem o filme se atualizam no presente. Arquivos de Havana, de Glauber Rocha, do cinema latino-americano, e de dimensões estéticas e políticas tensionadas e em relação com a linguagem cinematográfica e com os agenciamentos estabelecidos com o mundo. A partir da pesquisa bibliográfica e de uma análise fílmica ensaística, buscamos compreender como a forma do filme-ensaio é modulada e como os vestígios de imagens são mobilizados pela experiência fílmica do cineasta. A princípio, buscamos contextualizar o filme no âmbito do documentário contemporâneo brasileiro e aproximá-lo do conceito de filme-ensaio, percebendo seus contornos e camadas que se formam ao conceber o pensamento ensaístico como o norteador de nosso processo de análise fílmica. Em seguida, discutimos a as relações imbrincadas das imagens com a memória e a história possibilitadas pela montagem. A operação poética-construtiva da montagem marcada pela heterogeneidade de materiais e correspondências ganha destaque na figura do palimpsesto que convoca em forma e gesto a aporia de um passado que não foi, mas que é, e que continua se atualizando no presente.

No description available.

Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2017-04-03T19:37:47Z No. of bitstreams: 1 FlavioDeSousaRamalho_DISSERT.pdf: 3355624 bytes, checksum: 3f25f965b48c6134a1b1af73cc666a52 (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2017-04-05T18:17:54Z (GMT) No. of bitstreams: 1 FlavioDeSousaRamalho_DISSERT.pdf: 3355624 bytes, checksum: 3f25f965b48c6134a1b1af73cc666a52 (MD5) / Made available in DSpace on 2017-04-05T18:17:55Z (GMT). No. of bitstreams: 1 FlavioDeSousaRamalho_DISSERT.pdf: 3355624 bytes, checksum: 3f25f965b48c6134a1b1af73cc666a52 (MD5) Previous issue date: 2016-12-15 / O r?pido crescimento do n?mero de dispositivos conectados ? Internet, associado ?s taxas crescentes de popularidade e demanda de aplica??es e servi?os em tempo real na nuvem, com restri??es de lat?ncia, torna muito dif?cil para estruturas de computa??o em nuvem tradicionais acomod?-los de forma eficiente. Mais especificamente, a abordagem centralizada adotada tradicionalmente por Data Centers (DC) atuais apresentam problemas de desempenho para atender de aplica??es em nuvem com alta densidade, principalmente quanto a capacidade de resposta e escalabilidade. Nossa depend?ncia insubstitu?vel por computa??o em nuvem, exige infra-estruturas de DCs sempre dispon?veis, enquanto mant?m ao mesmo tempo capacidades de desempenho suficientes para responder a uma enorme quantidade de solicita??es de aplicativos em nuvem. Neste trabalho, a aplicabilidade do emergente paradigma de computa??o em n?voa ? explorada para melhorar o desempenho no suporte de aplica??es em nuvem sens?veis ? lat?ncia voltadas a Internet das Coisas (do ingl?s Internet of Things - IoT). Com base neste objetivo, apresentamos o novo modelo denominado Infraestrutura de Borda como um Servi?o (do ingl?s Edge Infrastructure as a Service - EIaaS), que procura oferecer um novo modelo de computa??o em nuvem com servi?o de entrega baseado em computa??o de borda voltado a atender de forma eficiente as exig?ncias de aplica??es IoT em tempo real sens?veis ? lat?ncia. Com a abordagem EIaaS, provedores de nuvem podem implantar dinamicamente aplica??es/servi?os IoT diretamente nas infra-estruturas de computa??o de borda, nem como gerir seus recursos de n?vem/rede em tempo de execu??o, como forma de manter as aplica??es IoT sempre melhor conectadas e melhor servidas. A abordagem resultante ? arquitetada em uma estrutura modular, tendo como base tecnol?gica ferramentas de Rede Definida por Software (do ingl?s, Software- Defined Networking - SDN) para lidar com recursos de computa??o de borda (CPU, mem?ria, etc.) e de rede (caminhos, largura de banda, etc.), respectivamente. Os resultados preliminares mostram como as principais t?cnicas de virtualiza??o utilizadas no ?mbito deste trabalho, afetam o desempenho das aplica??es na infraestrutura de borda da rede. A virtualiza??o por containers leva vantagem sobre a t?cnica de virtualiza??o por m?quinas virtuais para implantar aplica??es na borda da rede, uma vez que oferece grande flexibilidade mesmo na presen?a de demanda de recursos. / The rapid growth in the number of Internet-connected devices, associated to the increasing rates in popularity and demand for real-time and latency-constrained cloud application services makes the use of traditional cloud computing frameworks challenging to afford such environment. More specifically, the centralized approach traditionally adopted by current Data Center (DC) pose performance issues to suit a high density of cloud applications, mainly in terms to responsiveness and scalability. Our irreplaceable dependency on cloud computing, demands DC infrastructures always available while keeping, at the same time, enough performance capabilities for responding to a huge amount of cloud application requests. In this work, the applicability of the fog computing emerging paradigm is exploited to enhance the performance on supporting latency-sensitive cloud applications tailored for Internet of Things (IoT).With this goal in mind, we introduce a new service model named Edge Infrastructure as a Service (EIaaS), which seeks to offer a new edge computing tailored cloud computing service delivery model to efficiently suit the requirements of the real-time latency-sensitive IoT applications. With EIaaS approach, cloud providers are allowed to dynamically deploy IoT applications/services in the edge computing infrastructures and manage cloud/network resources at the run time, as means to keep IoT applications always best connected and best served. The resulting approach is modeled in a modular architecture, leveraging both container and Software-Defined Networking technologies to handle edge computing (CPU, memory, etc) and network resources (path, bandwidth, etc) respectively. Preliminary results show how the virtualization technique affects the performance of applications at the network edge infra. The container-based virtualization takes advantage over the hypervisor-based technique for deploying applications at the edge computing infrastructure, as it offers a great deal of flexibility under the presence of resource constraints.

No description available.

Made available in DSpace on 2014-12-17T15:01:56Z (GMT). No. of bitstreams: 1 AlessandroALA_TESE.pdf: 2166690 bytes, checksum: 87b921d322f56a3c11d668a0ad8d9341 (MD5) Previous issue date: 2013-11-11 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior / The nonionic surfactants when in aqueous solution, have the property of separating into two phases, one called diluted phase, with low concentration of surfactant, and the other one rich in surfactants called coacervate. The application of this kind of surfactant in extraction processes from aqueous solutions has been increasing over time, which implies the need for knowledge of the thermodynamic properties of these surfactants. In this study were determined the cloud point of polyethoxylated surfactants from nonilphenolpolietoxylated family (9,5 , 10 , 11, 12 and 13), the family from octilphenolpolietoxylated (10 e 11) and polyethoxylated lauryl alcohol (6 , 7, 8 and 9) varying the degree of ethoxylation. The method used to determine the cloud point was the observation of the turbidity of the solution heating to a ramp of 0.1 ? C / minute and for the pressure studies was used a cell high-pressure maximum ( 300 bar). Through the experimental data of the studied surfactants were used to the Flory - Huggins models, UNIQUAC and NRTL to describe the curves of cloud point, and it was studied the influence of NaCl concentration and pressure of the systems in the cloud point. This last parameter is important for the processes of oil recovery in which surfactant in solution are used in high pressures. While the effect of NaCl allows obtaining cloud points for temperatures closer to the room temperature, it is possible to use in processes without temperature control. The numerical method used to adjust the parameters was the Levenberg - Marquardt. For the model Flory- Huggins parameter settings were determined as enthalpy of the mixing, mixing entropy and the number of aggregations. For the UNIQUAC and NRTL models were adjusted interaction parameters aij using a quadratic dependence with temperature. The parameters obtained had good adjust to the experimental data RSMD < 0.3 %. The results showed that both, ethoxylation degree and pressure increase the cloudy points, whereas the NaCl decrease / Os tensoativos n?o i?nicos, quando em solu??o aquosa, apresentam a propriedade de separa??o em duas fases, sendo uma denominada de fase dilu?da, baixa concentra??o em tensoativo, e a outra rica em tensoativo denominada de coacervato. A aplica??o deste tipo de tensoativo nos processos de extra??o de solutos de meios aquosos vem aumentando o que implica na necessidade do conhecimento das propriedades termodin?micas desses tensoativos. Neste trabalho foram determinados os pontos de n?voa dos tensoativos polietoxilados, da fam?lia do nonilfenolpolietoxilado variando o grau de etoxila??o (9,5;10;11;12 e 13), da fam?lia do octilfenolpolietoxilado variando o grau de etoxila??o (10 e 11) e do ?lcool laur?lico polietoxilado nos graus de etoxila??o (6;7;8 e 9). O m?todo utilizado para determina??o do ponto de n?voa foi a observa??o do turvamento da solu??o ao aquecer o sistema a uma rampa de 0,1oC/minuto, e no estudo da press?o foi utilizada uma c?lula de alta press?o, m?ximo (300 bar). Atrav?s dos dados experimentais obtidos dos respectivos tensoativos, foram utilizados os modelos de Flory-Huggins, UNIQUAC e NRTL para descrever as curvas do ponto de n?voa dos referidos tensoativos. Foram tamb?m estudados a influ?ncia da concentra??o do NaCl e da press?o no ponto de n?voa. Este ?ltimo par?metro ? importante para os processos de recupera??o avan?ada de petr?leo nos quais os tensoativos em solu??o s?o submetidos a press?es elevadas. Enquanto que o efeito do NaCl permite obter pontos de n?voa a temperaturas mais pr?xima da temperatura ambiente viabilizando sua utiliza??o em processos sem controle de temperatura. O m?todo num?rico utilizado para ajustar os par?metros foi o Levenberg-Marquardt. Para o modelo de Flory-Huggins foram determinados par?metros de ajuste como a entalpia da mistura, entropia da mistura e o n?mero de agrega??o. Para os modelos UNIQUAC e NRTL foram ajustados par?metros de intera??o aij utilizando uma depend?ncia quadr?tica com a temperatura. Os par?metros obtidos tiveram bons ajustes aos dados experimentais com RMSD < 0,3%. Os resultados mostraram que tanto a etoxila??o quanto a press?o aumentam o ponto de n?voa do tensoativo, enquanto que o NaCl diminui

No description available.