Currently, asset management has been a big concern of business science. However, few studies focused on asset valuation of the business of information technology (IT) in government offices, especially with regard to its replacement by outsourcing process. Therefore, this study aims at developing a mathematical model for analyzing the economic feasibility of projects outsourcing of information technology in a public organization (in this case, the UFSM), with a focus on service printing. Thus, from a case study of a descriptive-quantitative, with interviews with managers of the organization and outsourcing companies, it was possible to apply the model with three printing equipment belonging to the institution, chosen because of high performance and productivity, which is identified in each case the best time of substitution (useful lives) and the proposal most advantageous exchange, the economic point of view. Moreover, the sector in which the print job has been outsourced was possible to compare the costs of the company that currently provides this service with other outsourcing options researched the market, so finding the most viable organization. / Atualmente, a gestão patrimonial tem sido uma grande preocupação das ciências empresariais. No entanto, são poucos os estudos voltados à avaliação de ativos do ramo de tecnologia da informação (TI) em órgãos públicos, principalmente em relação ao seu processo de substituição por terceirização. Assim, este trabalho tem como objetivo geral desenvolver um modelo para a análise da viabilidade econômico-financeira de projetos de terceirização de TI em uma organização pública específica, com foco no serviço de impressão. Através de um estudo de caso, com o método do Custo Anual Uniforme Equivalente como ferramenta de apoio e a realização de entrevistas junto a gestores da organização e de empresas de outsourcing, foi possível aplicar o modelo junto a três equipamentos de impressão pertencentes à instituição, escolhidos por serem de alto desempenho e produtividade, onde se identificou a vida útil econômica de cada ativo (3 anos para a Impressora 1, 4 anos para a Impressora 2 e 5 anos para a Impressora 3), e a proposta de troca mais vantajosa, do ponto de vista econômico: terceirização ou compra de um novo equipamento (em todos os casos, as opções de terceirização mostraram-se mais viáveis). Além disso, no setor em que o serviço de impressão já era terceirizado foi possível comparar os custos da empresa que executa atualmente esse serviço com as demais opções de terceirização pesquisadas, de modo a encontrar a mais viável à organização (a Opção B para substituir dois equipamentos grande porte e Opção C para substituir uma de médio porte). Palavras-chave: Atualmente, a gestão patrimonial tem sido uma grande preocupação das ciências empresariais. No entanto, são poucos os estudos voltados à avaliação de ativos do ramo de tecnologia da informação (TI) em órgãos públicos, principalmente em relação ao seu processo de substituição por terceirização. Assim, este trabalho tem como objetivo geral desenvolver um modelo para a análise da viabilidade econômico-financeira de projetos de terceirização de TI em uma organização pública específica, com foco no serviço de impressão. Através de um estudo de caso, com o método do Custo Anual Uniforme Equivalente como ferramenta de apoio e a realização de entrevistas junto a gestores da organização e de empresas de outsourcing, foi possível aplicar o modelo junto a três equipamentos de impressão pertencentes à instituição, escolhidos por serem de alto desempenho e produtividade, onde se identificou a vida útil econômica de cada ativo (3 anos para a Impressora 1, 4 anos para a Impressora 2 e 5 anos para a Impressora 3), e a proposta de troca mais vantajosa, do ponto de vista econômico: terceirização ou compra de um novo equipamento (em todos os casos, as opções de terceirização mostraram-se mais viáveis). Além disso, no setor em que o serviço de impressão já era terceirizado foi possível comparar os custos da empresa que executa atualmente esse serviço com as demais opções de terceirização pesquisadas, de modo a encontrar a mais viável à organização (a Opção B para substituir dois equipamentos grande porte e Opção C para substituir uma de médio porte).

Due to the high cost of production, some farmers have abandoned their activities and invested in other, with the factors mismanagement of your business and the lack of specific technologies to its development, coupled with lack of a study that points the technical and economic feasibility insertion of these technologies. This study's main objective is to point out the technical / economic feasibility of the implementation of the Precision Farming System (PA) in the rice crop planted in the pre-germinated system in the city of Cachoeira do Sul/RS, without the need for machinery acquisition specific nor skilled labor, with simple techniques that can be performed by the manufacturer or the technician who has knowledge in AP. An experimental area of 42 hectares has been evaluated with sampling of 1 hectare grids and application of nutrients to variable rate through physical demarcation (banners) of management zones. Plants were harvested mechanically and productivity was inferred through the manual harvest estimation methods that have proven effective. The cost of production in the area of traditional cultivation system was R$ 5,412.21 ha-1, with a revenue of R$ 8,457.88 ha-1 and in precision agriculture cultivation system R$ 5,241.01 ha-1, with a revenue of R$ 8,401.40 ha-1, with the PA system increased profitability (R$ 114.72 ha-1) compared to the traditional system, showing the technical feasibility / economic / environmental deployment the Precision Farming system at the study site. / Devido ao alto custo de produção, alguns agricultores têm abandonado suas atividades e investido em outras, tendo como fatores a má gestão de seu negócio e a falta de tecnologias específicas ao seu empreendimento, aliada a inexistência de um estudo que aponte a viabilidade técnica e econômica de inserção dessas tecnologias. Este estudo tem por principal objetivo apontar a viabilidade técnica/econômica da implantação do sistema de Agricultura de Precisão (AP), na cultura do arroz plantado no sistema pré-germinado no município de Cachoeira do Sul/RS, sem a necessidade de aquisição de maquinário específico e nem mão-de-obra especializada, com técnicas simples que podem ser executadas pelo próprio produtor ou pelo técnico que possua conhecimento em AP. Foi avaliada uma área experimental de 42 hectares, com grids amostrais de 1 hectare e aplicação de nutrientes a taxa variável, através de demarcação física (bandeirolas) das zonas de manejo. A colheita foi realizada de forma mecânica e a produtividade foi inferida através dos métodos de estimativa de colheita manual que se mostraram eficientes. O custo de produção da área no sistema de cultivo tradicional foi de R$ 5.412,21 ha-1, com uma receita de R$ 8.457,88 ha-1 e no sistema de cultivo de agricultura de precisão de R$ 5.241,01 ha-1, com uma receita de R$ 8.401,40 ha-1, apresentando o sistema de AP uma maior rentabilidade (R$ 114,72 ha-1) em relação ao sistema Tradicional, mostrando a viabilidade técnica/econômica/ambiental da implantação do sistema de Agricultura de Precisão no local do estudo.

Baseline data on a species' distribution and abundance are essential for developing practical conservation management plans. Such data are difficult to obtain for many low density cryptic carnivores. The Scottish wildcat, Felis silvestris silvestris, is no exception with <400 individuals thought to remain. Its conservation has been further complicated by extensive hybridisation and introgression with the domestic cat (F.s.catus). Hybridisation has also resulted in difficulties in discriminating between wildcats, wildcat x domestic hybrids (hybrids) and tabby coloured feral domestic cats. This has inhibited survey efforts, leading to a lack of general ecological information. Using the most recent identification tools available, extensive surveys using various methods including camera trapping were carried out across Northern Scotland in order to examine the current status of the Scottish wildcat. Current distribution indicates a more restricted range than recent studies. Wildcats are at risk of hybridisation from feral domestic cats and in particular, hybrids, throughout their current probable range. The distribution of hybrids overlaps with both feral domestic cats and wildcats, pointing to a significant threat from hybrids acting as a bridge between wildcats and feral cats. Mean density estimates of 3.5 (SD=0.7) wildcats/100 km² were comparable with those from other studies in Scotland using different survey methods. Total population size estimates ranged between 115-314 individuals depending on local densities and home range size. Population viability analysis (PVA) indicated the current population is not viable unless management actions are undertaken in the near future (Mean time to extinction = 48.2 years (SD = 9.39), probability of extinction=1, SE = 0), and that reducing mortality rates and/or supplementing populations from captive bred cats are likely to be necessary to achieve viability. Based on these data, the Scottish wildcat may be more endangered than many other species classified as Endangered and the current status of the Scottish wildcat should be reviewed.

Magister Scientiae (Medical Bioscience) - MSc(MBS) / Eurycoma Longfolia Jack, also known as Tongkat Ali (TA) is a tropical plant belonging to the family of Simaroubaceae is widely distributed in South East Asian countries. The extracts of TA have been proven to have cytotoxicity, anti-proliferative and aphrodisiac properties. In vitro assays revealed cytotoxicity toward human breast cancer cell lines MCF-7, KB, CaOV-3, RD, DU-145and HepG2 human liver cancer cells and appear promising as a new chemotherapeutic agent against human cervical carcinoma (HeLa) cells. Although, there are extensive studies reported on its cytotoxicity benefits there are none pertaining to LNCaP human prostate cell line. Therefore, this study aimed at testing the effects of TA on LNCaP cells and prostate specific antigen (PSA) production. Materials and Methods This study investigated the effect of different concentrations of TA (0.0025, 0.025, 2.5, 25 and 250 g/ml) TA on LNCaP human prostate cancer cell line for 24 and 96 hours. The following parameters were investigated: morphology, cell viability (MTT), testosterone modulation, Annexin V-CY3 binding (Apoptosis), DNA fragmentation (TUNEL), caspase 3/7 activity (apoptosis), and PSA production. Results When observing the morphological changes of LNCaP cells exposed to TA, a clear increase in detachment and cell death via apoptosis as the concentrations of TA increased. The viability decreased significantly in both 24 and 96 hour treatment of TA at higher dosages (25 and 250 g/ml). The significant inhibitory effects on testosterone stimulated cell proliferation were seen at TA concentrations as low as 0.0025 μg/ml TA. At higher concentrations of TA (25 and 250 μg/ml), for all testosterone dosages a decreasing trend in proliferation was found. vii Testosterone concentrations of 10 nM showed maximum stimulation of cell proliferation for TA dosages up to 2.5 μg/ml. All concentrations of TA showed significant increase in apoptosis of the cells as dosages increased. A higher amount of DNA damage found at the highest dosage (250 μg/ml) of TA. The relative caspase 3/7 activation showed significant (P=0.0043) activation at the highest concentration (250 μg/ml) of TA. Relative PSA production resulted only a 5% increase with no significant difference at all doses indicting that TA does not change the cell PSA production and the decline in PSA concentration is due to LNCaP cells dying as a result of this exposure to TA. Conclusion In summary, the major finding of this study is that Tongkat Ali inhibits the viability of prostate cancer cell lines (LNCaP) through caspase-mediated pathway, as well as increased the level of apoptotic such as DNA fragmentation. In addition, Tongkat Alin also inhibited PSA production. In LNCaP cells, testosterone with the addition of TA does not increase the growth of the cells. However, more in-vitro and in-vivo studies are needed to establish the exact constituents of the extracts and their mechanism of action. Thus, this study opens perspectives on the use of Tongkat Ali preparations in the treatment of aging male symptoms, prostate cancer prevention or as additional treatment to standard prostate cancer therapy.

>Magister Scientiae - MSc / Cissampelos capensis, is commonly known by the Afrikaans name ‟dawidjies” or ‟dawidjieswortel”. C. capensis is the most important and best known medicinal plant of the family Menispermaceae used by the Khoisan and other rural people in the western regions of South Africa. Among numerous other ailments, it is traditionally taken to treat male fertility problems. Yet, no studies have investigated the effects of this plant or its extracts on human spermatozoa. The aim of study was to investigate the effects of C. capensis rhizome extracts on sperm function.

The development and implementation of effective species and population-specific management strategies requires population-specific information. To demonstrate the relative extirpation risk associated with various road mortality scenarios for a population of Blanding’s turtles at Canadian Nuclear Laboratories in Chalk River, Ontario, a Population Viability Analysis was conducted. Road mortality of two adult females every ten years resulted in population extirpation within 200 years relative to a stable population not experiencing road mortality. To accommodate informed decision-making for the management of this species at risk, the movement patterns and habitat selection of this Blanding’s turtle population were described. There was no significant difference between males and females in distance moved between relocations in either the spring or the summer, but turtles moved greater distances in the spring than in the summer. Annual and seasonal home range size did not differ between the sexes or between spring and summer periods. A compositional analysis indicated Blanding’s turtles preferred marsh habitats over bog, swamp, lake, and upland. Matched-paired logistic regression was used to determine selection of microhabitat features, such as type of vegetation, in the spring and summer. Turtles preferred sites with warmer air temperatures, shallower water, a higher availability of open water, and greater coverage of emergent and floating vegetation types in the spring period. In the summer period, turtles preferred sites characterized by cooler, deeper water, a higher availability of open water, and greater coverage of emergent and floating vegetation types. This population of Blanding’s turtles appears to be relatively small and the continued threat of road mortality indicates a delicate situation for its persistence. Considering seasonally preferred habitats will best inform management decisions for seasonal work restrictions and future development plans.

The basic aim of this dissertation is to evaluate the predictive ability and accuracy of the existing and until now created corporate models predicting financial distress and verification of multinational force models. Partial goals must be filled to meet the basic objective and therefore the theoretical part is especially mapping models used and developed in the Czech Republic and other European economies, of which the emphasis is primarily on the former transition economies. These now post-transition economies have passed a similar political and economic development as the Czech Republic, and therefore prediction models created and used there could be transferable to the environment of the Czech economy and Czech companies whose financial health and conditions are assessed. The practical part is based on the author's own research, which is dedicated to the evaluation of prediction models when applied to Czech business entities differentiated according to belonging to a particular industry branch. During the models' evaluation the emphasis is on the fact whether they are national (Czech) or foreign models.

Submitted by Fabio Sobreira Campos da Costa (fabio.sobreira@ufpe.br) on 2016-04-15T12:45:36Z No. of bitstreams: 2 license_rdf: 1232 bytes, checksum: 66e71c371cc565284e70f40736c94386 (MD5) Dissertação Emanuela Paz Rosas Final.pdf: 1301979 bytes, checksum: 812637acf4c6404700fd08c4dbcc4009 (MD5) / Made available in DSpace on 2016-04-15T12:45:36Z (GMT). No. of bitstreams: 2 license_rdf: 1232 bytes, checksum: 66e71c371cc565284e70f40736c94386 (MD5) Dissertação Emanuela Paz Rosas Final.pdf: 1301979 bytes, checksum: 812637acf4c6404700fd08c4dbcc4009 (MD5) Previous issue date: 2015-08-27 / CAPEs / Os produtos finais de glicação avançada (AGEs) são compostos heterogêneos gerados a partir da reação não enzimática, irreversível, conhecida como reação de Maillard ou Glicação. Essa reação ocorre entre açúcares redutores e seus metabólitos reativos ou lipídeos oxidados e proteínas ou ácidos nucléicos. Os AGEs são formados endógena (hiperglicemia e estresse oxidativo) e exogenamente (alimentos e fumo), e podem ser acumulados em vários tecidos causando efeitos deletérios em diversas patologias relacionadas a idade e a diabetes. Uma de suas principais ações é gerar estresse oxidativo através do aumento da expressão de mediadores inflamatórios. Os macrófagos são células fundamentais no reconhecimento, degradação e remoção dessas substâncias, além disso, têm papel crucial na produção de espécies reativas de nitrogênio (RNS) na resposta imune, e podem estar envolvidos com variadas patologias. Neste estudo avaliou-se o efeito dos AGEs sobre a produção de óxido nítrico e a viabilidade celular em macrófagos. Macrófagos J774 foram incubados com diferentes concentrações de BSA- AGE (15, 30, 60, 120 ou 240μg/mL) por 24 ou 48 h e em seguida foram expostas (ou não) ao LPS (100ng/ml) por 24h. Para analisar a produção de óxido nítrico e a viabilidade celular utilizaram-se os métodos de Griess e de MTT, respectivamente. As células J774 submetidas ao tratamento com AGE apresentaram alterações nos níveis de NO e na viabilidade celular. Notou-se que os macrófagos quando incubados com AGE nas maiores concentrações (60, 120, 240μg/mL), sem LPS, produziram um aumento significativo na liberação de NO por 24 h. Também foi visto que o tratamento com AGE nas concentrações 120 e 240μg/mL, e com LPS, também elevaram os níveis de NO significativamente. Os resultados mostraram um aumento na produção de NO quando as células foram tratadas com AGE em todas as concentrações (15, 30, 60, 120 e 240μg/mL) por 48 h. A incubação de células J774 com AGE nas concentrações de 240 μg/ml por 24h com LPS, como também as concentrações de 120 e 240 μg/ml por 48h sem LPS, reduziram a viabilidade celular quando comparados com seus respectivos controles. Em conclusão, os AGEs podem causar aumento da produção de NO por macrófagos quando estes são estimulados ou não por LPS, e por consequência conduzir a morte celular. / The advanced glycation end products (AGEs) are heterogenic compounds generated from Maillard reaction or Glycation reaction. It is an irrevesible nonenzymatic reaction involving reductive sugars and reactive metabolites or oxidated lipids, proteins or nucleic acids. AGEs could be originated by endogenous way (hyperglycemia or oxidative stress) and by exogenous way (certain types of food and tobacco). They could be accumulated in several organic tissues causing deleterious effects seen in diabetes and age-related diseases. One of the main AGE effects is the induction and maintenance of stress oxidative through inflammatory mediators increasing expression. In this scenario, macrophages are fundamental cells on the recognition, degradation and clearance of AGEs. They also play an important role on immune response by stimulating reactive nitrogen species production (RNS), which could be involved on the evolution of several diseases. The present study evaluated the AGE effect on the macrophages nitric oxide production as well as the cell viability. J774 macrophages were incubated with different concentrations of BSA- AGE (15μg, 30μg, 60μg, 120μg ou 240μg/mL) by 24 or 48 hours, following a second incubation with or without LPS (100ng/mL) by 24 hours. Nitric oxide production was evaluated by Griess reaction and cell viability by MTT production. Macrophages incubated with AGE presented nitric oxide and cell viability alterations. It was noted that macrophages when incubated with AGE in higher concentrations (60, 120, 240μg/mL) without LPS produced a significant increase in the release of NO for 24 h. It has also been seen that treatment with AGE concentrations 120 and 240μg/mL and stimulation by LPS have also increased NO levels significantly. The results showed an increase in NO production when cells were treated with AGE at all concentrations (15, 30, 60, 120 and 240μg/ mL) for 48 h. The incubation J774 cells with AGE at concentrations of 240 μg/ mL for 24 hours with LPS, as well as the concentrations of 120 and 240 μg/ mL for 48h without LPS, reduced the cell viability compared to their respective controls. In conclusion, AGEs can cause increased production of NO by macrophages when these are stimulated or not with LPS, leading to cell death.

A típica produção de cana-de-açúcar por fornecedores autônomos no Estado de São Paulo dá-se em pequenas propriedades rurais. Nessa atividade, constatou-se prejuízo econômico entre as safras 2007/08 e 2011/12, fato este que leva ao objetivo do presente estudo: responder por que, então, tais produtores mantêm-se no fornecimento de cana-deaçúcar em detrimento de encerrarem seus projetos e direcionarem os recursos para outras atividades. Foram analisados dois projetos de investimento em cana-de-açúcar, tomando como base informações disponíveis das regiões de Piracicaba e Sertãozinho. Considerando-se o método do fluxo de caixa descontado, obtiveram-se estimativas que apontam para a atratividade econômica do plantio de cana em ambas as regiões, haja vista a resposta positiva dos Valores Presentes Líquidos calculados. Tais evidências foram corroboradas quando da utilização da Teoria das Opções Reais, proposta que incorpora no modelo de decisão a flexibilidade gerencial do investidor. Neste caso, mais expressivas foram as respostas obtidas em termos de atratividade financeira. Ressalta-se, ainda, para os principais parâmetros que impactam na viabilidade econômica de empreendimentos canavieiros, a saber, o preço e a qualidade da cana-de-açúcar e a produtividade agrícola. Adicionalmente, os resultados apontam para melhores condições financeiras quando a produção de cana é realizada em terras próprias - em detrimento de áreas arrendadas - além da necessidade de incentivos dados pelas usinas aos fornecedores da região de Sertãozinho. Medidas que visam ganhos de produtividade agrícola e a profissionalização da atividade são apontadas como fatores motivadores de ganhos financeiros, enquanto que em termos de políticas públicas cita-se a possibilidade de ajustes nos sistemas de precificação da gasolina C e na tributação dos derivados da cana-de-açúcar, ações que tendem a elevar a rentabilidade da atividade ora analisada. / The typical production of sugarcane by independent suppliers in the São Paulo State gives up on small farms. In this activity, it was found that between crops 2007/08 and 2011/12 there was economic loss, a fact that leads to the goal of the present study: answer why these producers still remain providing sugarcane. Apart from the traditional viability analysis, the study incorporated the Real Options Theory analysis, which includes managerial flexibility on the investor decision model. Two sugarcane investment projects based on available information of Piracicaba and Sertaozinho regions were analyzed. Considering the discounted cash flow method, we obtained estimates pointing to the economic attractiveness of sugarcane planting in both regions, as shown by the positive response of the Net Present Values calculated. This evidence was corroborated when using the Real Options Theory, given the most significant were the responses in terms of financial attractiveness. Additionally, the results point to better financial conditions when the production of sugarcane is held in its own land, beyond the need for mills incentives to independent suppliers of Sertaozinho. Price and quality of sugarcane and agricultural productivity presented the higher impact on the financial viability of projects. Finally, actions focus on agricultural productivity gains are seen as motivating for financial gain, while in terms of public policy we mention the possibility of adjustments in C gasoline pricing systems and in the taxation on derived products from sugarcane.

Made available in DSpace on 2016-01-26T18:56:03Z (GMT). No. of bitstreams: 1 Deigilam Cestari Esteves.pdf: 2776358 bytes, checksum: 7e1a46b5b264cbf8e0c0d96e68b501e2 (MD5) Previous issue date: 2014-12-18 / The environmental and hospital infections, caused by bacteria which are resistant to a wide variety of antibiotics, have shown increasing records in the last years, manifesting themselves in high mortality and lethality. Recent researches report that the bacteria presents a survival profile on dry surfaces in order to maintain their virulence when exposed to biological fluids such as urine, saliva and blood. The objective of this study was to document - through laboratorial analysis the survival capacity of the main bacteria of medical interest on abiotic surfaces. The adopted procedures were completely conducted in the microbiology laboratory at Unoeste, in Presidente Prudente SP. Standard ATCC strains of Staphylococcus aureus (ATCC25923), Enterococcus faecalis (ATCC29212), Escherichia coli (ATCC35218), Klebsiella pneumoniae (ATCC700603) and Pseudomonas aeruginosa (ATCC27583) were used, to which biological fluids and water were added. The surfaces were tile, synthetic fabric, mattress and cotton fabric. The surfaces were contaminated with suspension composed by the ATCC strains, biological fluids and water in addition to the control containing only the ATCC strains, which were stored in petri dishes, kept in room temperature. Every seven days the surfaces were dipped in trypticase soy broth and kept in the oven for 24h at 37ºC. They were spread from the contaminated brothin Mueller Hinton agar and kept for 24h in the oven at 37ºC. The viability analysis was done through the colony-forming unity (CFU) counting. For the statistical analysis, the Software R was used, through the Friedman and Kruskal Wallis tests for multiple comparisons, analyzing within each factor (solution or surface) which differ between one another (Friedman s test) and also which treatments interactions are different (Kruskal-Wallis). Through this analysis it was possible to observe that in the current work that Staphylococcus aureus kept its viability for a longer period than other microorganisms under all the tested conditions, presenting significant differences between the fluids and surfaces, with a particular colony growth in blood and cotton. The Klebsiella pneumoniae showed differences between fluids, with saliva containing the highest colony number. With regards to the other tested bacteria, there was no isolated significance. For the multiple comparisons analysis, only the Pseudomonas aeruginosa presented no significant difference between any pair of treatments, whereas the other bacteria presented significant differences between the correlations. The need to analyze the environmental impact of these microorganisms persistence in environments, which are vulnerable to human beings, guides the creation of measures in order to control the spread of pathogenic microorganisms. / As infecções ambientais e hospitalares causadas por bactérias resistentes a um amplo espectro de antibióticos têm índices crescentes nos últimos anos, manifestando-se com alta morbidade e letalidade. Pesquisas recentes evidenciam que as bactérias demonstram um perfil de sobrevivência, em superfícies secas de modo a manter sua virulência quando expostas a fluidos biológicos como urina, saliva e sangue. O objetivo desse estudo foi documentar através de análises laboratoriais a capacidade de sobrevivência das principais bactérias de interesse médico em superfícies abióticas. Os procedimentos foram totalmente realizados no laboratório de microbiologia da Unoeste em Presidente Prudente - SP., utilizando cepas padrão ATCC de Staphylococcus aureus(ATCC25923), Enterococcus faecalis (ATCC29212), Escherichia coli(ATCC35218), Klebsiella pneumoniae(ATCC700603) e Pseudomonas aeruginosa(ATCC27583), ao qual foram adicionados fluídos biológicos e água. As superfícies utilizadas foram piso, tecido sintético, colchão e tecido algodão. As superfícies foram contaminadas com suspensão composta pelas cepas ATCC, fluídos biológicos e água, além do controle contendo somente as cepas ATCC, armazenadas em placas de petri e mantidas em temperatura ambiente. A cada sete dias as superfíciesarmazenadas foram mergulhadas em caldo Tripscaseína de Soja (TSB), colocadas na estufa por 24h a 37ºC. Foram semeados do caldo contaminado em ágar Mueller Hinton e mantidos por 24h em aquecimento a 37º em Estufa.A análise da viabilidadefoi realizada através da contagem de unidade formadora de colônias (U.F.C.). Para a análise estatística utilizou o software R, realizando os testes de Friedman e de Kruskal Wallis para comparações multiplas, analisando dentro de cada fator (solução ou superfície) quais diferem entre si (Teste de Friedman) e, também, quais interações de tratamentos são diferentes (Kruskal-Wallis). Através dessas análises foi possível observar no presente trabalho que Staphylococcus aureus manteve a viabilidade por tempo maior que os outros microrganismos em todas as condições testadas, apresentando diferença significativa entre os fluídos e as superfícies, tendo sangue e tecido algodão crescimento de maior número de colônias. A Klebsiella pneumoniae apresentou diferença entre os fluídos, sendo asaliva com maior número de colônias. Para as outras bactérias testadas não houve significância isoladamente. Para as análises de comparações múltiplas somente para a Pseudomonas aeruginosanão houve diferença significativa entre algum par de tratamentos, as outras bactérias apresentaram diferenças significativas entre as correlações. A necessidade de analisar o impacto ambiental da persistência desses microrganismos em ambientes vulneráveis ao ser humano, norteia o delineamento de medidas para o controle na disseminação de microrganismos patogênicos.