This research investigate the matrix effect, operation time, cost and solvent comsumption for triarylmethanes and corresponding metabolites in different aquaculture products by different extraction methods. Triarylmethane was determined by LC-ESI-MS/MS in positive mode. The analytical method validation were followed the regulation of 2002/657/EC. HPLC separation was conducted with the RP-18 column. The mobile phases consisted of 0.5 mM ammonium acetate buffer (pH 3.8, adjusted with acetic acid) ¡V acetonitrile (contained 0.1% formic acid) solution. The ratios of standard deviation to average residual time for all compounds are less than 0.05. The ion ratio is stable and allowed by the regulation of 2002/657/EC. The regression constant of calibration curve is in the range from 0.998 to 0.999. The CC£\ for MG, LMG, CV, LCV are 0.09 ¡Ó 0.05, 0.19 ¡Ó 0.14, 0.15 ¡Ó 0.13, 0.15 ¡Ó 0.10 £ggkg-1, respectively. The CC£] for MG, LMG, CV, LCV are 0.09 ¡Ó 0.05, 0.19 ¡Ó 0.14, 0.15 ¡Ó 0.13, 0.15 ¡Ó0.10 £ggkg-1, respectively. The results for SPE and liquid-liquid extraction are not significantly different; the liquid-liquid extraction have shorter operation time, cheaper cost and less solvent consumption in comparison with SPE.

In this research, two major experiments, including the self-assembly of silica spheres, were performed by using a physical confinement method with an attractive capillary force. The silica spheres were dragged and aggregated as results of the evaporation of the solvent. In the first experiment, silica spheres were assembled into the two-dimensional pattered substrate, constructed by the photo-resist film formed under a lithography process. Several patterned substrates could work as a physical trap during the flow of the silica spheres. The ordered arrangement of the silica spheres was controlled by the concentration and the size of the silica spheres, the thickness of the photo-resist film, and the titled angle of the substrate. In our conditions, the silica spheres could orderly arrange in larger area of the substrate. In the second experiment, a surface-enhanced Raman scattering (SERS) enhancement was observed from a chain of silica spheres with silver nanoparticles, which worked as a excitation source to provid a strong local electromagnetic fields exciting the crystal violet (CV) dye coated on the silica spheres. We found that the CV molecules has a strong SERS intensity due to the refraction and reflection of the incident light within the silica spheres. When the silica spheres were linearly arranged, longer length of the chained silica spheres would lead to a maximum value of the SERS intensity.

The presence of flowers is felt in Catholic architecture, literature, artwork, personal histories and devotional practices. This, however, has not always been the case. The Catholic Church has had a long and tumultuous relationship with flowers, the focus of which has been the subject of considerable scholarship (e.g. Fisher (2011, 2007), Ward (1999), Winston-Allen (1997), Goody (1993), Coats (1970)). What has not been much considered is a phenomenological treatment of Catholic floral experience, and how such experiences have shaped individual and shared understandings of the Catholic faith. This thesis seeks to redress this omission through an exploration of the life of the Polish Catholic mystic, St. Faustina Kowalska (1905-1938), whose mystical experiences with the divine were explicitly mediated and narrated through flowers. Through Faustina’s diary, Divine Mercy in my Soul, we gain access to powerful, and unequivocally Catholic, experiences with flowers which comprise the very centre of her religious convictions. This thesis queries the ways in which flowers have dynamically shaped, and have been shaped by, St. Faustina's relationship with God and Catholic holy figures. To address this question I use the semiotic, phenomenological and hermeneutic approach of Max van Manen. Van Manen uses four elements of lived experience he calls lifeworld existentials, these are: lived space, lived time, lived body and lived relationality. These four categories are applied to St. Faustina’s life as she engages with God spatially, temporally, corporeally and relationally; each reveals the centrality of flowers in her religious experiences. While this thesis focuses on the religio-floral experiences of a particular mystic-saint, its significance lies also in the broader Catholic narrative of which it is a part. Writing about flowers was a transformative medium in Faustina's life and has been historically significant in the lives of many other Catholic saints and mystics who recorded similar experiences. This thesis, in describing the details of St. Faustina’s floral-saturated experiences from her diary, reveals a particularized instance of a paradigmatic Catholic phenomenon whereby flowers provide access to the sacred.

Abstract: The role of bacterial biofilms in patients with persistent CRS is of growing concern. The limited efficacy of some medical and surgical treatments for CRS illustrates the need for further progress in this area. The current treatments of chronic rhinosinusitis are concentrated on medical +/- surgical therapy. In this thesis, we consider two methods performed in chronic rhinosinusitis, endoscopic sinus surgery and long term low-dose macrolide therapy, and consider how they can affect bacterial biofilms. The effect of endoscopic sinus surgery on bacterial biofilms and the clinical impact of this condition on CRS patients may be far more profound than we can currently understand. To understand the impact of ESS on bacterial biofilms, we have performed the first prospective study to evaluate the effect of ESS on bacterial biofilms in patients with CRS and patients’ clinical outcomes after 3 months follow-up. We have shown that ESS results in a statistically significant improvement in QoL, subjective and objective outcome measures. In terms of bacterial biofilms, the mean OD630nm of isolates was significantly reduced after 3 months follow-up (p=0.043). No correlations between the reduction of bacterial biofilms with any of the objective, subjective and QoL outcomes were seen in our study. Macrolides have demonstrated their anti-inflamatory effects in the treatment of diffuse panbronchiolitis, asthma, cystis fibrosis and chronic rhinosinusitis. In recent years, there are a number of in vitro studies supporting the anti-biofilm effects of macrolide antibiotics, especially at sub-MICs level. These have shown that macrolides alter the outer membrane, lipopolysaccharide of biomass and inhibit the expression of other bacterial virulence factors which may disrupt the adherence of bacteria to form biofilms. Long term low dose macrolide therapy, therefore, may transform bacterial biofilms from the protected organized form into the plantonic form. In this thesis, we also report the first in vivo efficacy of long term low dose macrolides on bacterial biofilms in patients with CRS. Patients receiving oral macrolide showed significant improvements in subjective, objective and QoL scores following a 12 week course. Nasal swabs were taken from CRS patients at the first visit and 3 months after macrolide therapy. Using the microtiter biofilm assay, these swabs showed a reduction in the mean OD630nm of isolates in 8/12 patients. While it is well-known that bacterial biofilms are established in CRS patients, the relationship between the improvement of clinical symptoms and the severity of bacterial biofilm is less clear.

Thesis (M.A.)--Bowling Green State University, 2007. / Document formatted into pages; contains viii, 71 p. Includes bibliographical references.

Cinzas oriundas da combustão de carvão são resíduos produzidos no processo de geração de energia em termoelétricas. Esses resíduos industriais podem ser aproveitados como matéria prima para síntese de zeólitas, as quais são materiais aluminossilicatos porosos com importantes aplicações, como adsorventes no tratamento de efluentes líquidos. Nesse contexto, o objetivo deste trabalho foi sintetizar zeólitas a partir de três diferentes cinzas de carvão e utilizá-las na remoção de corante violeta cristal em soluções aquosas via processo de adsorção. Para isso, foram sintetizadas zeólitas denominadas ZP, ZF e ZFD a partir de três diferentes cinzas de carvão, sendo elas, respectivamente: cinzas de carvão de combustão em leito pulverizado, de combustão em planta piloto de leito fluidizado e de combustão em planta piloto de leito fluidizado utilizando processo de dessulfuração interna. A síntese foi feita pelo método hidrotérmico, utilizando NaOH 3 mol L-1 como agente mineralizante, relação líquido/sólido (L/S) igual a 6 e tempo de reação de 24 h a 100 °C. Os materiais obtidos foram submetidos à caracterização física, química e morfológica, nas quais foi observada a formação de três diferentes fases zeolíticas (zeólitas P, X e sodalita) nas amostras ZF e ZFD, e uma única fase (zeólita P) na amostra ZP. Os valores de área superficial BET encontrados foram de 17, 35 e 40 m2 g-1 para as zeólitas ZP, ZFD e ZF, respectivamente, com tamanho de poros na faixa de 3 a 4 nm para os três materiais. Os valores de capacidade de troca catiônica (CTC) ficaram entre 2,07 e 2,85 meq NH4 + g-1. Posteriormente foram realizados ensaios de adsorção do corante em que foi avaliada a influência do pH, tempo de contato e concentração de sólido adsorvente. Ainda, foram construídas isotermas de adsorção para esses sistemas e os modelos de isotermas de Langmuir, Freundlich e BET modificado foram ajustados aos dados experimentais. Os resultados mostraram que a maior eficiência foi obtida pela zeólita ZF, alcançando máxima remoção de corante de 85 % nos estudos do efeito do tempo de contato, e as melhores condições experimentais obtidas foram de 60 min e 15 g L-1 de concentração de sólido adsorvente. O modelo de isotermas que melhor se ajustou aos dados experimentais foi BET modificado, com R2 = 0,9894. A síntese de zeólita a partir de cinzas de carvão mostrou potencial na produção de um adsorvente alternativo para remoção do corante violeta cristal de águas contaminadas, contribuindo também para a diminuição do impacto ambiental das atividades de combustão de carvão. / Ashes from coal combustion are waste produced in the process of power generation in thermoelectric. These industrial wastes can be used as raw material for zeolite synthesis, which are porous aluminosilicate with important applications, such as adsorbents in the treatment of liquid effluents. The objective of this study was to synthesize zeolites from three different coal ash and apply it in crystal violet dye adsorption processes in aqueous solution. Thereunto, zeolites called ZP, ZF e ZFD were synthesized from the three different coal ashes, as so, respectively: coal ashes from powdered bed combustion, from combustion in a fluidized bed pilot plant and from combustion in a fluidized bed pilot plant with internal desulphurization. The synthesis was carried by hydrothermal method, using NaOH 3 mol L-1 as the mineralizing agent, the liquid/solid ratio (L/S) equal to 6 and 24 hours reaction time at 100 ° C. The materials were subjected to physical, chemical and morphological characterization and it was observed the formation of three different zeolite phases (zeolites P, X and sodalite) in the sample ZF and ZFD, and a single phase (zeolite P) in ZP sample. The BET surface area values were 17, 35 and 40 m2 g-1 to ZP, ZFD and ZF zeolites, respectively, with pore size in the range of 3 to 4 nm for all three materials. The values of cation exchange capacity (CEC) were between 2.07 and 2.85 meq NH4 + g-1. Subsequently, adsorption tests were carried out in which it was evaluated the influence of pH, contact time and adsorbent solid concentration. Still, adsorption isotherms were built for these systems and isotherm models of Langmuir, Freundlich and modified BET were fitted to experimental data. The results showed that the highest efficiency was obtained by zeolite ZF, achieving maximum dye removal of 85 % in the studies of contact time effects, and the best experimental conditions were 60 min and adsorbent solid concentration of 15 g L-1. The BET isotherm model was found to be the best fit to experimental data, with R2 = 0.9894. The zeolite synthesis from coal ash has shown potential in the production of an alternative adsorbent to remove the crystal violet dye from contaminated water, also contributing to reducing the environmental impact of coal combustion activities.

Made available in DSpace on 2014-06-11T19:34:59Z (GMT). No. of bitstreams: 0 Previous issue date: 2010-07-03Bitstream added on 2014-06-13T18:45:52Z : No. of bitstreams: 1 winckler_dcf_dr_botfca.pdf: 826861 bytes, checksum: 0065d6bc13ca46069b7bee48b655a961 (MD5) / Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) / A espécie Sarsina violascens (Herrich–Schaeffer, 1856) (Lepidoptera: Lymantriidae) é relatada como praga primária em plantios de eucalipto e teve sua primeira ocorrência registrada em 1975. No período de 2006 a 2009 houve grandes surtos dessa praga, causando desfolhamento intenso em plantios de eucalipto no norte da Bahia. Apesar dos estudos feitos no passado terem considerado espécies de eucalipto, há pouca informação sobre a suscetibilidade de clones híbridos de eucalipto à mariposa violácea. O presente trabalho teve por objetivo avaliar a biologia de S. violascens em diferentes espécies e híbridos de eucalipto e em diferentes temperaturas, além de avaliar a eficiência de entomopatógenos no controle de S. violascens. No experimento 1 a biologia de S. violascens foi determinada nas espécies Eucalyptus grandis, E. urophylla e dois híbridos de E. grandis x E. urophylla (‘urograndis’). Os parâmetros avaliados foram duração e viabilidade dos estágios larval, pupal, adulto e de ovo e do ciclo total. No experimento 2 foi selecionada a espécie E. urophylla como padrão e avaliou-se o efeito de diferentes temperaturas (18, 22, 26 e 30oC) no desenvolvimento de S. violascens, avaliando-se os mesmos parâmetros anteriores. Os resultados obtidos no experimento 1 revelaram que E. urophylla ofereceu melhores condições para odesenvolvimento de S. violascens, com maior viabilidade larval (85,3 %), pupal (78,7 %) e de ovos (72,6 %), e menor duração do ciclo total (54,4 dias). A espécie E. grandis foi considerada a menos adequada ao desenvolvimento de S. violascens, revelando baixas viabilidades larval (29,2 %) e pupal (28,9 %). Os resultados obtidos com os dois híbridos foram intermediários comparativamente com as espécies testadas. No experimento 2 as temperaturas de 18, 22 e 26°C foram adequadas para o desenvolvimento e reprodução de S. violascens, com alta viabilidade... / Defoliator lepidopterous are one of the main Eucalyptus pest groups in Brazil. There are records of caterpillars outbreaks since 1940, with important occurrences during 1980’s to 1990’s. The species Sarsina violascens (Herrich–Schaeffer, 1856) (Lepidoptera: Lymantriidae), named as violet moth, is related as pest in eucalypts plantations since 1975 and during 2006 to 2009, this species has occurred in outbreaks, causing intense defoliation in eucalypt plantations in Northern Bahia, Brazil. Despite studies in the past have considered species of eucalyptus, there is little information about susceptibility of Eucalyptus hybrid clones to violet moth. This study aimed to determine the biology of S. violascens in different species and hybrids of eucalyptus and in different temperatures. The insects were observed diary to evaluate life cycle in different species of eucalyptus (experiment 1) and in different temperatures (experiment 2). In experiment 1 were tested the following species: Eucalyptus grandis, E. urophylla and two hybrids of E. grandis x E. urophylla (‘urograndis’). The parameters evaluated were: duration and viability of larvae, pupae, adult and eggs phases and total life cycle. In experiment 2 was chose the species E. urophylla as standard and evaluated the effect of temperatures of 18, 22, 26 and 30oC, considering the same previous parameters. The results obtained in experiment 1 showed that E. urophylla offered better conditions to S. violascens development and reproduction, due the higher larvae, pupae and eggs viability, and shorter total life cycle duration. The species E. grandis was considered as less adequate to S. violascens development and reproduction, with low larvae and pupae viability. To the two hybrid clones, the results were intermediary comparatively with tested species. In experiment 2 temperature of 18°C showed the most adequate to S. violascens... (Complete abstract click electronic access below)

Cinzas oriundas da combustão de carvão são resíduos produzidos no processo de geração de energia em termoelétricas. Esses resíduos industriais podem ser aproveitados como matéria prima para síntese de zeólitas, as quais são materiais aluminossilicatos porosos com importantes aplicações, como adsorventes no tratamento de efluentes líquidos. Nesse contexto, o objetivo deste trabalho foi sintetizar zeólitas a partir de três diferentes cinzas de carvão e utilizá-las na remoção de corante violeta cristal em soluções aquosas via processo de adsorção. Para isso, foram sintetizadas zeólitas denominadas ZP, ZF e ZFD a partir de três diferentes cinzas de carvão, sendo elas, respectivamente: cinzas de carvão de combustão em leito pulverizado, de combustão em planta piloto de leito fluidizado e de combustão em planta piloto de leito fluidizado utilizando processo de dessulfuração interna. A síntese foi feita pelo método hidrotérmico, utilizando NaOH 3 mol L-1 como agente mineralizante, relação líquido/sólido (L/S) igual a 6 e tempo de reação de 24 h a 100 °C. Os materiais obtidos foram submetidos à caracterização física, química e morfológica, nas quais foi observada a formação de três diferentes fases zeolíticas (zeólitas P, X e sodalita) nas amostras ZF e ZFD, e uma única fase (zeólita P) na amostra ZP. Os valores de área superficial BET encontrados foram de 17, 35 e 40 m2 g-1 para as zeólitas ZP, ZFD e ZF, respectivamente, com tamanho de poros na faixa de 3 a 4 nm para os três materiais. Os valores de capacidade de troca catiônica (CTC) ficaram entre 2,07 e 2,85 meq NH4 + g-1. Posteriormente foram realizados ensaios de adsorção do corante em que foi avaliada a influência do pH, tempo de contato e concentração de sólido adsorvente. Ainda, foram construídas isotermas de adsorção para esses sistemas e os modelos de isotermas de Langmuir, Freundlich e BET modificado foram ajustados aos dados experimentais. Os resultados mostraram que a maior eficiência foi obtida pela zeólita ZF, alcançando máxima remoção de corante de 85 % nos estudos do efeito do tempo de contato, e as melhores condições experimentais obtidas foram de 60 min e 15 g L-1 de concentração de sólido adsorvente. O modelo de isotermas que melhor se ajustou aos dados experimentais foi BET modificado, com R2 = 0,9894. A síntese de zeólita a partir de cinzas de carvão mostrou potencial na produção de um adsorvente alternativo para remoção do corante violeta cristal de águas contaminadas, contribuindo também para a diminuição do impacto ambiental das atividades de combustão de carvão. / Ashes from coal combustion are waste produced in the process of power generation in thermoelectric. These industrial wastes can be used as raw material for zeolite synthesis, which are porous aluminosilicate with important applications, such as adsorbents in the treatment of liquid effluents. The objective of this study was to synthesize zeolites from three different coal ash and apply it in crystal violet dye adsorption processes in aqueous solution. Thereunto, zeolites called ZP, ZF e ZFD were synthesized from the three different coal ashes, as so, respectively: coal ashes from powdered bed combustion, from combustion in a fluidized bed pilot plant and from combustion in a fluidized bed pilot plant with internal desulphurization. The synthesis was carried by hydrothermal method, using NaOH 3 mol L-1 as the mineralizing agent, the liquid/solid ratio (L/S) equal to 6 and 24 hours reaction time at 100 ° C. The materials were subjected to physical, chemical and morphological characterization and it was observed the formation of three different zeolite phases (zeolites P, X and sodalite) in the sample ZF and ZFD, and a single phase (zeolite P) in ZP sample. The BET surface area values were 17, 35 and 40 m2 g-1 to ZP, ZFD and ZF zeolites, respectively, with pore size in the range of 3 to 4 nm for all three materials. The values of cation exchange capacity (CEC) were between 2.07 and 2.85 meq NH4 + g-1. Subsequently, adsorption tests were carried out in which it was evaluated the influence of pH, contact time and adsorbent solid concentration. Still, adsorption isotherms were built for these systems and isotherm models of Langmuir, Freundlich and modified BET were fitted to experimental data. The results showed that the highest efficiency was obtained by zeolite ZF, achieving maximum dye removal of 85 % in the studies of contact time effects, and the best experimental conditions were 60 min and adsorbent solid concentration of 15 g L-1. The BET isotherm model was found to be the best fit to experimental data, with R2 = 0.9894. The zeolite synthesis from coal ash has shown potential in the production of an alternative adsorbent to remove the crystal violet dye from contaminated water, also contributing to reducing the environmental impact of coal combustion activities.

Orientador: Douglas Soares Galvão / Tese (doutorado) - Universidade Estadual de Campinas, Instituto de Fisica Gleb Wataghin / Made available in DSpace on 2018-08-10T18:28:40Z (GMT). No. of bitstreams: 1 Sato_Fernando_D.pdf: 7711328 bytes, checksum: 22c01d9be26cdd21cf062f6b8f62cde0 (MD5) Previous issue date: 2007 / Resumo: Neste trabalho utilizamos métodos de física computacional para descrever o comportamento estrutural de formação de cadeias atômicas lineares suspensas (LACs) (i ) e de uma estrutura orgânica sobre superfície metálica (ii ). Ambos os temas foram desenvolvidos sobre o ponto de vista de física computacional e comparados com resultados experimentais específicos provenientes da estreita colaboração em grupos experimentais. Desenvolvemos uma metodologia para estudo sistemático da formação de nanofios (i ) metálicos a partir de aglomerados (clusters) compostos por átomos puros (Au, Ag, Cu) e a partir de ligas bi-metálicas (Au-Ag). A metodologia se baseou nas equações de movimento de Newton (método de dinâmica molecular clássica) utilizando um potencial parametrizado de origem quântica, denominado potencial de ligações fortes com aproximação de segundos momentos (TB-SMA). Com a metodologia desenvolvida foi possível realizar um estudo estatístico da formação de cadeias atômicas lineares suspensas, a verificação de aglomerados até então somente vistos em estudos direcionados a clusters, a observação de defeitos de empilhamento e a previsão de novas estruturas. Estudamos aspectos da geometria de uma molécula orgânica denominada Violeta Lander (ii ) (VL) com métodos clássicos e semi-empíricos no vácuo. A VL é uma das moléculas da classe das moléculas de Lander. Após a descrição geométrica, colocamos a VL sobre uma superfície de Cu[110] para verificar posições e conformações de estabilidade através de métodos clássicos de mecânica molecular. Para entender o comportamento dinâmico da VL sobre uma superfície de Cu[110] utilizamos um método clássico de dinâmica molecular utilizando o potencial Universal Force Field (UFF). A relevância desta parte do trabalho reside no fato de investigarmos, pela primeira vez, um caso análogo ao efeito chave-fechadura não biológico estudado no ambiente experimental e teórico de simulação. Ambos os trabalhos nos remetem para o campo da nanociência, servindo de base para futuras aplicações em nanotecnologia, tais como contatos elétricos e interação de sistemas orgânicos sobre superfície metálica / Abstract: In this work we use tools of computational physics to describe the structural and formation behavior of metallic nanowires (i ) and an organic structure on metallic surface (ii ). Both subjects had been developed under computational physics point of view and compared to specific experimental data from our close collaborations with experimental and theoretical groups. In this part of the work we developed a methodology for systematic study of the formation of metallic nanowires (i ) from clusters composed by pure atoms (Au, Ag and Cu) and from bi-metallic alloys (Au-Ag). The methodology was based on the Newton equations of motion (traditional method of molecular dynamics) together with a parametrized potential of quantum origin, named Tight-binding potential with second moment approximations (TB-SMA). With the developed methodology it was possible to carry out a statistical study of suspended linear atomic chains formation from clusters and to predict new structures and defects, stacking faults, among others. We also studied aspects of the geometry of an organic molecule called Violet Lander (VL) (ii ) with classic and semi-empirical methods in vacuum and also deposited over copper [110]. The VL is one of molecules of the Lander molecules class. The great relevance of this part of the work is because we addressed and explained the rst non-biological lock-key eect in the experimental and theoretical environment. Both the works have a great nanoscience appeal, being one of its bases. It will have great importance in future applications of nanotechnology as electric contacts and organic-metallic interaction based devices / Doutorado / Física da Matéria Condensada / Doutor em Ciências

Submitted by Nadir Basilio (nadirsb@uninove.br) on 2017-04-27T18:10:59Z No. of bitstreams: 1 Cristina Silveira da Silva.pdf: 1525168 bytes, checksum: c8777d95365a6771207361a1332fe0e7 (MD5) / Made available in DSpace on 2017-04-27T18:10:59Z (GMT). No. of bitstreams: 1 Cristina Silveira da Silva.pdf: 1525168 bytes, checksum: c8777d95365a6771207361a1332fe0e7 (MD5) Previous issue date: 2016-08-31 / In cutting process, the cutting fluids are used for lubrication and cooling. However, its composition rich in organic components and the use of water as solvent make the fluid a favorable environment for the microorganisms proliferation. One of the ways to control this contamination is using bactericides, that over time, become a problem, both for the operator and for the part / machine. Ultraviolet radiation, which is widely known for its action on the partial elimination of microorganisms on surfaces, can be quite efficient in controlling or even eliminating contaminants. In order to contribute to the theme, this work aims to treat cutting fluid through the application of ultraviolet radiation. For this, a device specially developed for this purpose was used. In order to verify the efficiency of the procedure, the flow rate and the number of lamps used were changed through a previous experiment planning. The results of the tests confirmed the efficiency of the use of ultraviolet light together with related device, to controlling the microorganisms present in the cutting fluid. / Em usinagem os fluidos de corte são utilizados para lubrificação e refrigeração. Porém, sua composição rica em componentes orgânicos e a utilização de água como solvente tornam o fluido um ambiente favorável para a proliferação de microrganismos. Uma das maneiras de se controlar essa contaminação é a adição de bactericidas que ao longo do tempo se tornam um problema, tanto para o operador quanto para a peça/máquina. A radiação por ultravioleta que é amplamente conhecida por sua ação na eliminação parcial de microrganismos em superfícies, pode ser bastante eficiente no controle ou até mesmo eliminação dos contaminantes. Com o intuito de contribuir com o tema, este trabalho objetiva tratar fluido de corte por meio da aplicação de luz ultravioleta. Para isto, foi utilizado um dispositivo especialmente desenvolvido para tal finalidade. Para verificar a eficiência do procedimento, foram alterados, por meio de um planejamento de experimentos, a vazão e a quantidade de lâmpadas utilizadas. Os resultados dos testes realizados confirmaram a eficiência do emprego da luz ultravioleta, juntamente com o referido dispositivo, no controle dos microrganismos presentes no fluido de corte utilizado.