Osmoregulation is an essential process to maintain water and ionic balance and when euryhaline fish move between freshwater and seawater environments as part of their life cycle this presents additional osmoregulatory challenges. Migrating fish can be exposed in both environments to pollutants such as endocrine disrupting chemicals (EDCs) that include natural hormones (e.g. 17β-estradiol; E2), synthetic hormones (e.g. 17α-ethinylestradiol; EE2), and industrial chemicals (e.g. nonylphenol). The focus of this thesis was to study the effects of different categories of EDCs on the osmoregulatory functions of euryhaline fish such as three-spined sticklebacks (Gasterosteus aculeatus) and rainbow trout (Oncorhynchus mykiss). Osmoregulatory variables (such as osmolality, water and ionic content) were compared in plasma and tissues (white muscle and carcass) of rainbow trout. This validated the use of specific tissue parameters as a surrogate of plasma responses to various osmoregulatory challenges. Waterborne exposure to 17α-ethinylestradiol revealed differential sensitivity of vitellogenesis in the three-spined sticklebacks (no induction) and rainbow trout, but had a significant effect on calcium homeostasis in both species. Intraperitoneal implants of 17β-estradiol reduced CaCO3 production and apparent water absorption in the intestine and increased in tissue calcium stores of seawater-acclimated trout, but fish were able to compensate and showed no overall osmoregulatory disturbance. Waterborne exposure to nonylphenol in freshwater trout was also investigated, but no effects on osmoregulation were found up to 2 ng/l. Overall, estrogens can affect osmoregulation differentially in euryhaline fish species, and sometimes at EDC levels lower than the threshold for reproductive effects (i.e. vitellogenin induction).

De nombreuses recherches se focalisent sur l'étude des aires de distribution des espèces qui se décalent vers des conditions plus adaptées à leurs besoins physiologiques sous l'effet du changement climatique. Le choix des indices utilisés pour caractériser l'écologie des espèces et définir leur vulnérabilité au réchauffement en cours est souvent conditionné par leur disponibilité, alors qu'il devrait être basé sur les connaissances en écophysiologie qui les concernent. D'autre part, la résolution spatiale parfois grossière utilisée n'est pas toujours pertinente au regard de l'échelle à laquelle les processus biologiques se déroulent. Dans ce cadre, l'objectif de ce travail est de cartographier à fine résolution spatiale les bilans en eau des sols et leurs différentes composantes à l'échelle des forêts de France, et d'évaluer leur intérêt pour modéliser la distribution ou la productivité des espèces au regard des indices traditionnellement utilisés. Dans un premier temps, nous avons modélisé et cartographié les différentes composantes du bilan en eau des sols, et tout particulièrement le rayonnement solaire et la réserve utile maximale en eau (RUM) des sols forestiers à partir des relevés de l'Inventaire Forestier National (IFN). Ces données ont été combinées avec des températures et des précipitations pour spatialiser le bilan en eau des sols forestiers de France. Les principaux résultats montrent l'importance de la nébulosité dans la prise en compte du calcul du rayonnement solaire, et l'inefficacité des indices dérivés de l'exposition pour en simuler les valeurs à l'échelle de la France. Nous avons également déterminé qu'il est possible de réaliser avec des informations simples à collecter une carte des RUM des sols forestiers de France. Elle permet de prédire la croissance des essences avec une efficacité comparable aux valeurs relevées sur des placettes et d'améliorer la modélisation de la distribution de certaines essences. Enfin, nous démontrons que les calculs de bilans en eau qui prennent en compte la réserve en eau des sols sont plus efficaces que les bilans hydriques climatiques ou les pluies, particulièrement pour ce qui concerne les espèces hygrophiles ou xérophiles. Ces résultats laissent penser que l'importance de l'eau a été sous-estimée dans l'analyse de la distribution des espèces et l'étude des conséquences du changement climatique sur les plantes. Les données produites permettent de progresser dans la connaissance de l'écologie des espèces et de mieux caractériser la vulnérabilité des espèces, ouvrant la porte à la création d'outils plus fonctionnels pour aider les gestionnaires à évaluer les impacts du changement de climat et à s'y adapter. / Numerous researches focus on species distribution shifts toward ecological conditions most suited to plants under climate change. Ecological indices used to characterize species ecology and to define their vulnerability over broad areas are often at coarse resolution and are determined by data availability. The aim of this work was to map soil water balance and its different components at a fine spatial resolution, and to evaluate their interest to model plant distribution and growth over the whole French forests. We firstly modeled and mapped the solar radiation and the soil water holding capacity of forest soils. These data were combined with temperatures and precipitation to map the soil water balance. For solar radiation, the main results showed that this parameter is only accurately predicted at the French scale when cloudiness is taken into account. We also showed that soil water holding capacity can be mapped at the French scale using the basic information collected on numerous plots from the French national forest inventory. Values extracted from the soil water holding capacity map allowed predicting tree species growth with efficiency similar to values estimated on plots. We also demonstrated soil water balance is more efficient than climatic water balance or precipitation to model species distribution, mainly for hygrophilous and xerophilous species. These results suggest importance of available water could be underestimated when determining the ecological niche of species. These maps allow to improve species ecology knowledge and to help in the determination of their vulnerability area to climate change.

Ce travail de thèse a porté sur la prise en compte de la phase juvénile des peuplements forestiers du Sud-Ouest de la France dans la caractérisation des échanges sol-végétation-atmosphère. L’étude s’est focalisée sur des structures de peuplements contrastées de jeunes peuplements de Pins maritimes et d’Eucalyptus. La première partie de ces recherches a consisté à étudier de façon expérimentale les échanges de masse et d’énergie de jeunes plantations d’Eucalyptus et de Pins maritimes du Sud-Ouest. Trois sites instrumentés pour la détermination en continu des échanges de CO2 et H2O, bilan d’énergie, flux de sève et de croissance et production des arbres ont été suivis durant deux années marquées par des épisodes répétés de sécheresse. Le comportement des essences présentes a été comparé ainsi que les effets de l’environnement et des pratiques culturales sur le fonctionnement de ces écosystèmes. Les bilans annuels d’eau, de carbone et la croissance et la production des trois peuplements ont été établis et comparés. A partir de ces mesures enrichies par des données déjà disponibles et en se basant sur le modèle de production forestière et de transfert sol-végétation-atmosphère GRAECO (INRA-EPHYSE), modèle mécaniste biophysique monodimensionnel, la deuxième partie a consisté à développer une nouvelle évolution de ce modèle pour l’adapter au cas des systèmes de culture de biomasse forestière à croissance rapide. L’approche proposée en couplant ce modèle avec le modèle MAESTRA (Medlyn 2004) a permis de dépasser les hypothèses d’homogénéités verticale et horizontale du couvert pour rendre compte de l’effet de la structure en trois dimensions de peuplements jeunes à couronnes disjointes et sous-étage développé sur les transferts radiatifs, de CO2 et vapeur d’eau. De plus, le modèle a été complété par trois nouveaux modules décrivant respectivement la dynamique du carbone du sol (Roth-C) et la croissance et fonctionnement du sous-étage et du taillis. Il a été évalué sur les données existantes couvrant différents sites et séries temporelles et incluant une série d’interventions de type labour, désouchage, disquage superficiel, semis, dépressages, éclaircies et recépées. Enfin, ce modèle a été implémenté sur un jeu de données décrivant le climat local sous le forçage du scénario climatique A2 régionalisé sur un point de la grille SAFRAN de Météo-France pour trois itinéraires sylvicoles de Pin maritime et Eucalyptus, afin d’évaluer, à titre exploratoire, les potentialités de productivité de ces itinéraires. / In this thesis, the juvenile phase of forest stands of southwestern France was studied in order to characterise soil-vegetation-atmosphere exchanges. The study focused on contrasted structures of young pines and Eucalyptus stands. The first part of this research was to study experimentally the mass and energy exchanges of young plantations of pines and Eucalyptus growing in southwestern France. Continuous measurements of CO2 and H2O fluxes, energy balance, sapflow and tree growth and production were carried out for two years, a period marked by repeated episodes of drought. The behavior of these species was compared, as well as the effects of the environment and cultural practices on these ecosystems. The annual water and carbon balances, growth and production of the three stands were established and compared. These measurements were enriched by data already available and were based on the 1D-mechanistic model of forest production and soil-vegetation-atmosphere transfer GRAECO (INRA-EPHYSE). The second part of the thesis focused on developing a new version of the model to be applied to crop systems for fast-growing forest biomass. By coupling this evolution of the model with the 3D-model MAESTRA (Medlyn 2004), the assumptions of vertical and horizontal homogeneities in the canopy can be exceeded thus accounting for the effect of the three-dimensional structure on the radiative, CO2 and water vapor transfers in young stands, where crowns are separate and the understorey is well-developed. In addition, the model was supplemented by three new modules describing the dynamics of soil carbon (Roth-C) and the growth and functioning of the understory and coppice. It was evaluated on existing data covering different sites and time series and including a series of forest practices, such as plowing, stump removal, superficial disking, seeding, early thinning, thinnings and cutting down close to the ground. Finally, as an exploratory work, this model has been implemented on a data set describing the local climate forced by the A2 climate scenario which was regionalized on a SAFRAN grid point (‘Météo-France’) for three forest itineraries of maritime pine and Eucalyptus, in order to assess the potential productivity of these systems.

L'un des enjeux majeurs en écologie est de comprendre et prédire la réponse des organismes aux variations environnementales. Pour cela, la clarification des mécanismes proximaux est une étape indispensable pour comprendre des patrons écologiques généraux comme l'utilisation de l'habitat ou la distribution des espèces. Dans ce contexte, l'approche écophysiologique est particulièrement pertinente. Si la ressource trophique et les contraintes énergétiques ont attiré un intérêt considérable, les compromis liés à l'eau demeurent actuellement peu considérés. Pourtant l'eau est aussi une ressource capitale pouvant être limitante. La régulation de la balance hydrique pourrait donc jouer un rôle clé dans les compromis physiologiques et comportementaux.L'objectif principal de ce doctorat est d'évaluer l'importance des compromis liés à l'eau chez une espèce caractéristique des milieux bordiers (haies, lisières), la vipère aspic (Vipera aspis). Cette espèce vivipare est particulièrement dépendante des conditions thermiques, notamment pour sa reproduction. Notre hypothèse générale est qu'au même titre que les conditions thermiques, les conditions hydriques confrontent l'organisme à des compromis physiologiques et comportementaux importants, particulièrement lors de la reproduction (i.e., gestation). Nous avons combiné des études descriptives (thermorégulation, pertes hydriques) et expérimentales (manipulation de l’accès à l'eau) qui suggèrent l'existence d'un compromis entre la thermorégulation et la balance hydrique. Ces contraintes liées à l'eau sont associées à des ajustements physiologiques et comportementaux qui doivent être considérés pour comprendre les stratégies reproductrices et identifier de possibles conflits intergénérationnels (mère-embryons). Au même titre que les contraintes énergétiques ou thermiques, les besoins en eau sont donc essentiels à considérer pour aborder des questions écologiques et évolutives générales. / A major goal in ecology is to understand and predict species responses to environmental variations. Clarifying the proximate factors involved is a crucial step to unravel general ecological patterns such as habitat use or species distribution. In this context, the use of an ecophysiological approach can be particularly relevant. Trophic resource and energy tradeoffs attracted considerable interest but water-based tradeoffs remain relatively overlooked to date. However water is a critical, often limiting resource that must be considered. The regulation of water balance may have a key influence on physiological and behavioral tradeoffs. The main objective in this thesis is to evaluate the importance of water-based tradeoffs in a species characteristic of "ecotone" habitats (hedgerows, edges), the Aspic viper (Vipera aspis). This species is viviparous and highly depends on thermal conditions during reproduction. Our general hypothesis is that, as thermal conditions, hydric conditions should expose individuals to important physiological and behavioral tradeoffs, especially during reproduction (i.e., pregnancy).We combined descriptive (thermoregulation, water losses) and experimental studies (manipulation of water availability) that suggest a significant tradeoff between thermoregulation and water balance regulation. Water-based tradeoffs induce physiological and behavioral adjustments that are relevant to understand reproductive strategies and identify possible transgenerational (mother-embryos) conflicts. As energy or thermal resource, water should therefore also be considered to address ecological or evolutive questions.

O Sistema Nacional de Informações de Recursos Hídricos (SNIRH) ainda não monitora todos os rios brasileiros. Esta carência de informações reflete em falhas na estimativa da disponibilidade hídrica para os usos múltiplos da água. Nesse contexto, os modelos hidrológicos destacaram-se por tentar suprir essa insuficiência de dados. Entretanto, nota-se que muitos modelos não geram bons resultados quando executados fora da área de estudo onde foram criados. Por isso, devido à flexibilidade na escolha dos processos hidrológicos a serem simulados, este trabalho utilizou o framework JAMS (Jena Adaptable Modelling System) na construção de um modelo chuva-vazão específico para a bacia piloto do Ribeirão da Onça, a qual está inserida em zona de afloramento de um dos mais importantes aquíferos do mundo, o Sistema Aquífero Guarani (SAG). O JAMS apresentou-se como um sistema simplificado e flexível diante das adaptações necessárias para tratar e gerar dados compatíveis com as características climatológicas e hidrológicas da área de estudo. O modelo construído mostrou-se eficiente na predição da resposta hidrológica da bacia avaliada, apresentando um coeficiente de Nash-Sutcliffe de 0,76 e 0,81, para os períodos de calibração e validação do modelo, respectivamente. Foi ainda satisfatória a predição do volume escoado, apresentando uma porcentagem de viés de -1.13 e -1.03% para os dois períodos. A reconstituição da série histórica de vazão mostrou que o escoamento de base representa de 79 a 89% do escoamento total anual da bacia do Ribeirão da Onça. Portanto, o modelo construído torna-se uma importante ferramenta para a reconstituição da série histórica dos dados fluviométricos da bacia do Ribeirão da Onça, importante para o aperfeiçoamento dos estudos de recarga e proteção do SAG. / The National System of Information Resources (SNIRH) has not monitored all Brazilian rivers. This lack of information reflects in the fault estimation of water availability for the multiple uses. In this context, the hydrological models stood out for trying to address this lack of data. However, it is noted that many models do not generate good results when performed outside of the study area where they were created. Therefore, due to flexibility in the choice of hydrological processes to be simulated, this study used the framework JAMS (Jena Adaptable Modelling System) in the construction of a specific rainfall-runoff model to the basin of the Ribeirão da Onça monitored watershed, which is embedded in an outcrop area of one of the most important aquifers in the world, the Guarani Aquifer System (SAG). The JAMS presented as a simplified and flexible for use on the necessary adaptations to process and generate consistent data with the climatological and hydrological characteristics of the study area. The constructed model was effective in predicting the hydrological response of the basin evaluated, with a Nash-Sutcliffe coefficient of 0.76 and 0.81, for periods of calibration and validation of the model, respectively. It was also satisfactory prediction of runoff volume, with a bias of -1.3 and -1.03% for the two periods. The runoff time series reproduction showed that the baseflow represents from 79 to 89% of the annual total runoff in the Ribeirão da Onça watershed. Therefore, the constructed model becomes an important tool for reconstruting the time series fluviometric data from Ribeirão da Onça basin, important for the improvement of the recharge studies and SAG protection.

Injúria renal aguda (IRA) é doença de elevada incidência, associada a altas taxas de morbimortalidade. Sepse, pós-operatório de grandes cirurgias e baixo débito cardíaco são as principais causas de IRA em todo o mundo. Na maioria destas situações, expansão volêmica é parte do manejo preventivo e terapêutico da IRA. Contudo, a manutenção de uma estratégia de infusão liberal de fluidos pode causar balanço hídrico positivo (BH+), que tem sido associado a desfechos desfavoráveis em pacientes criticamente enfermos. BH+ frequentemente ocorre nestes pacientes que recebem grandes infusões de volume, mesmo que apresentem volume urinário considerado satisfatório ou acima de 0,5ml/kg/h. Nesta situação, se não houver elevação da creatinina sérica, não será feito o diagnóstico de IRA pelos critérios do Kidney Disease Improving Global Outcome (KDIGO), ainda que haja claro déficit na eliminação da sobrecarga hidrossalina. Este estudo observacional prospectivo, com controle pareado por dias de exposição ao BH+ avaliou a associação entre BH+ e diagnóstico subsequente de IRA (pelos critérios do KDIGO) e mortalidade em 233 pacientes admitidos em uma unidade de terapia intensiva (UTI) geral. Observamos por análise de regressão logística que cada 100 ml de aumento no BH se associou a elevação de 4% na chance de desenvolver IRA (OR 1,04; IC 95% 1,01 a 1,08). Comparado ao primeiro quartil de BH médio, o quarto quartil de BH médio (BH > +1793 ml/dia) se associou a chance 3,12 vezes maior de desenvolver IRA (OR 3,12; IC 95% 1,13 a 8,65). Comparado ao BH de zero até +1500 ml/dia, o BH médio > +1500 ml/dia se associou a chance 3,4 vezes maior de desenvolver IRA, (OR 3,4; IC 95% 1,56 a 7,48). Um modelo de efeito fixo mostrou que BH+ estava presente pelo menos seis dias antes do diagnóstico de IRA pelos critérios do KDIGO. Para avaliar o desfecho óbito, consideramos o BH durante toda internação na UTI. Observamos que cada 100 ml de aumento no BH se associou a incremento de 7% na mortalidade (OR 1,07; IC 95% 1,02 a 1,12). Comparado ao primeiro quartil, o quarto quartil de BH médio (BH > +1652 ml/dia) se associou a chance 2,8 vezes maior de evoluir para óbito (OR 2,8; IC 95% 1,04 a 7,66). Comparado aos pacientes com BH de zero a +1500 ml/dia, os pacientes com média de BH > +1500 ml/dia apresentavam chance 3,8 vezes maior de evolução para óbito (OR 3,8; IC 95% 1,55 a 9,16). Em conclusão, BH+ como variável contínua, em quartis ou utilizando ponto de corte maior do que +1500 ml/dia se associou de maneira independente a maior chance de desenvolvimento subsequente de IRA e evolução para óbito em pacientes criticamente enfermos. No presente trabalho, o BH + foi biomarcador precoce de IRA. Estes achados sugerem que BH+ deve ser incluído nos critérios de definição de IRA, ao lado da creatinina e diurese / Acute kidney injury (AKI) is a disease with high incidence, which is associated with high morbidity and mortality rates. Sepsis, major surgery and low cardiac output are the main causes of AKI worldwide. In the majority of these situations, volume expansion is part of both prevention and therapeutic management of AKI. However, maintaining liberal fluid infusion strategy can cause fluid overload and it is associated to poor outcomes in critically ill patients. Positive fluids balance (FB) frequently occurs in these patients receiving high volume infusion, even if the urinary output is adequate (above 0.5ml/kg/h). In this situation, if there is no serum creatinine (SCr) increase, AKI will not be diagnosed by current Kidney Disease Improving Global Outcome (KDIGO) criteria, even with a clear kidney inability to eliminate the body excess of fluid. This prospective, paired control, cohort study aimed to evaluate the association between positive FB and subsequent development of AKI by KDIGO criteria and mortality in 233 critically ill adults. By multiple logistic regression, we showed that each 100 ml increase in FB was independently associated to a 4% increase in the chances for developing subsequent AKI (OR 1.04; 95% CI 1.01 to 1.08). When compared to the first quartile, the fourth FB quartile (FB > +1793ml/day) was associated with a 3.12 times greater chance of developing AKI (OR 3.12; 95% CI 1.13 to 8.65). Compared to FB zero to 1,500ml/24h, the mean FB above +1,500 ml/24h was associated with an OR of 3.4 for AKI (OR 3.4; 95% CI 1.56 to 7.48). A mixed effect model demonstrated that a positive FB predicted AKI development defined by KDIGO criteria within 6 days. To assess the outcome mortality, we evaluated the mean FB during the whole ICU hospitalization. Each 100 ml increase in FB was associated to a 7% increase in the chances for death (OR 1.07; 95% CI 1.02 to 1.12). Compared to the first quartile, patients in the fourth FB quartile (FB > +1652 ml/day) showed an OR of 2.8 for death (OR 2.8; 95% CI 1.04 to 7.66). Mean FB above +1,500 ml/24h was associated with an OR of 3.8 for death, as compared to FB zero to 1,500ml/24h (OR 3.8; 95% CI 1.55 to 9.16). In conclusion, positive FB, as continuum variable, as quartiles and as absolute thresholds, was independently associated with subsequent AKI development and death in critically ill patients. In this study, the positive FB was early biomarker of AKI. These findings suggest that positive FB should be included in the criteria for AKI in addition to serum creatinine and urine output

O conhecimento do armazenamento de água pelo solo (ARM) é importante por tratarse de uma cultura de grande interesse agronômico. Os tensiômetros de polímero são novos dispositivos capazes de verificar a tensão de água no solo em situações de solo bem seco e realizam medidas do potencial matricial do solo que podem ser utlizadas para o cálculo de ARM. O presente trabalho tem por objetivos: (i) estimar o armazenamento de água no solo através dos métodos de estimativa de balanço hídrico de Thornthwaite e Mather (M), Rijtema e Aboukhaled (R) e Cossenoidal (C), combinados com a evapotranspiração de cultura estimada pelos métodos de Thornthwaite, Penamn e Penman-Monteith, a fim de compara-los com valores de ARM medidos com tensiômetros de polímeros; (ii) estimar a produtividade deplecionada por água e comparar com os dados observados em um experimento de campo com uma cultura da soja. O experimento foi conduzido no município de Piracicaba, SP, com tensiômetros instalados a 0,05, 0,15 e 0,30 m de profundidade, representando as camadas de 0-0,1, 0,1-0,2 e 0,2-0,4 m, respectivamente. De acordo com os resultados obtidos, conclui-se que: (i) o método M subestima a evapotranspiração atual ( ). Consequentemente, superestima o déficit hídrico e subestima S pois assume que (a) só é igual à evapotranspiração potencial quando ARM é igual à capacidade de armazenamento do solo (CAD); (b) M assume aumento linear entre nulo até a ; (ii) os métodos R e C apresentam estimativas de equivalentes; consequentemente, também apresentam estimativas equivalentes de e déficit hídrico. Porém, enquanto o método R assume aumento linear de na faixa de nulo até crítico, o método C assume variação sigmoidal (o que condiz mais com a realidade); (iii) o método de estimativa de evapotranspiração que melhor ajustou-se aos dados reais foi o de Penman e Monteith. Porém, quando apenas houver dados de temperatura disponíveis, recomenda-se a utilização do método de Thornthwaite; (iv) a capacidade de campo pode ser determinada experimentalmente com base no valor médio do potencial matricial ou umidade durante o período chuvoso; (v) para efeito de realização de balanço hídrico, a pode ser determinada com base na determinação prévia do ponto de murcha permanente (teor de água correspondente ao potencial matricial de -15 atm), profundidade efetiva do sistema radicular (profundidade do solo que contribui com 95% da evapotranspiração), com o coeficiente angular da reta igual a um; e (vi) a produtividade deplecionada por água da soja foi de 4279,7 kg ha-1, com uma quebra de 55% em relação à produtividade potencial. / The knowledge about soil water storage (ARM) is essential in agriculture mainly for crops of great agronomic importance. Polymer tensiometers are new instruments capable of measuring soil water tension in very dry soil conditions and perform measurements of soil matrix potential that can be use to calculate ARM. The aims of this study were: (i) to estimate ARM by the water balance estimation methods of Thornthwaite and Mather (M), Rijtema and Aboukhaled (R), and Cossenoidal (C), combined with crop evapotranspiration estimated by Thornthwaite, Penman and Penman-Monteith, in order to compare them with ARM values calculated from polymer tensiometer data; (ii) to estimate the water-depleted productivity of the soybean and compare it with observed data in a field experiment with a soybean crop. The experiment was conducted in Piracicaba, São Paulo State, Brazil, with tensiometers installed at 0.05; 0.15 and 0.3 m depths, representing 0-0.1; 0.1-0.2 and 0.2-0.4 m soil layers. The results show that: (i) the M method underestimates the actual evapotranspiration (ETa). Consequently, it overestimates the hydric deficit and underestimates ARM because it assumes that (a) ETa is only equal to the potential evapotranspiration when ARM is equal to the available soil water storage capacity (AWC); (b) M assumes a linear increase from null ARM until ; (ii) the R and C methods provide equivalent estimates of Eta; consequently, they also present equivalent estimates of ARM and hydric deficit. However, while R assumes a linear increase of Eta from null ARM until a critic ARM, C assumes a sigmoidal variation (which agrees more with reality); (iii) the best method of evapotranspiration estimative was M. However, when only temperature data is available, it is recommended to use the Thornthwaite method; (iv) the field capacity can be determined experimentally based on the average matric potential and moisture during the rainy season; (v) for calculating water balances, AWC can be determined based on the previous determination of the permanent wilting point (water content corresponding to the matric potential of -15 atm), effective depth of the root system (soil depth whichs contributes with 95% of the evapotranspiration), with the slope equal to one; and (iii) the water-depleted productivity of the soybeans was 4279.7 kg ha-1, 55% less than the potential productivity.

O balanço hídrico para a bacia do Ji-Paraná (RO) nos anos de 1995 e 1996 foi modelado de modo simples, integrado a um Sistema de Informações Geográficas (SIG), utilizando como entrada, imagens de sensores remotos orbitais, dados climáticos (precipitação) e informações quanto à cobertura e o tipo de solo da bacia. Imagens diárias do sensor AVHRR foram utilizadas na geração de imagens compostas mensais de temperatura da bacia, as quais foram utilizadas na estimativa da evapotranspiração potencial mensal, através do método empírico de Thornthwaite. Estas, juntamente com mapas de precipitação interpolados a partir de postos pluviométricos, foram então utilizadas para se efetuar o balanço hídrico da bacia conforme Thornthwaite – Mather. Todo o sistema está inserido em um SIG, de forma que os resultados são espacialmente distribuídos. A evapotranspiração média da bacia foi de aproximadamente 3,2 mm dia-1. A máxima ocorreu nos meses de outubro (aproximadamente 4,2 mm dia-1), período onde se tem tanto energia e água disponível para a evapotranspiração. A mínima ocorreu no auge da estação seca, nos meses de julho e agosto (aproximadamente 1,7 mm dia-1). Esta sazonalidade indica que o modelo é fortemente influenciado pela disponibilidade hídrica. Os resultados obtidos com a modelagem foram comparados com o balanço de massa anual de 10 sub-bacias presentes na bacia do Ji-Paraná. O modelo aqui proposto sub estimou ligeiramente a evapotranspiração real (aprox 9 %), principalmente no período seco, apontando que a restrição hídrica exerce muita influência sobre os resultados. No entanto, um melhor ajuste da capacidade de armazenamento de água do solo (CAD) pode levar a melhores resultados. / Water balance for the Ji-Paraná (RO) basin for 1995 and 1996 was done through a simple model, integrated into a Geographic Information System (GIS), using as input, remote sensing images, climatic data (precipitation), soils and land cover maps. Daily AVHRR images were used to obtain mean monthly temperature composites, which were converted to monthly potential evapotranspiration, using Thornthwaite’s empirical formulation. Along with precipitation maps, derived from the interpolation of rain gages, the water balance was obtained according to Thornthwaite – Mather approach. All modeling was done inside a GIS, allowing for the results to be spatially distributed. Mean evapotranspiration for the basin is approximately 3.2 mm day-1. Maximum evapotranspiration, close to 4.2 mm day-1, occurred in October, when there is sufficient water and available energy. Minimum evapotranspiration, approximately 1.7 mm day-1, occurred at the peak of the dry season, July and August. This indicates that the model is strongly influenced by water availability. The results obtained by the model were checked against annual water balances for 10 sub-basins in the Ji-Paraná basin. Evapotranspiration was sub-estimated by aprox. 9 %. However, a better adjustment of the water holding capacity (CAD) can lead to better results.

O déficit de irrigação tem sido utilizado como uma das mais vantajosas estratégias de economia de água, redução dos custos com irrigação e custo de oportunidade de água. O objetivo principal deste trabalho foi avaliar o efeito da irrigação plena e com déficits em duas épocas distintas, no crescimento da planta, nas relações hídricas e parâmetros de produção e qualidade da pimenta cv. Tabasco (Capsicum frutescens). Como objetivo secundário avaliar o método da sonda de dissipação térmica (SDT) na estimativa da transpiração da pimenta Tabasco. O experimento foi desenvolvido em ambiente protegido, no Departamento de Engenharia de Biossistemas na Escola Superior de Agricultura Luiz de Queiroz ESALQ-USP, em Piracicaba-SP, de setembro de 2009 a agosto de 2010. O delineamento foi em blocos casualizados, com quatro repetições, lâminas de irrigação de 40, 60, 80 e 100% da evapotranspiração da cultura (Etc), inicializadas aos 39 dias após o transplantio - DAT (E1) e aos 59 DAT (E2). Aferiu-se a altura da planta (AP) e o comprimento de fruto (CF), o diâmetro do fruto (DF) e do caule da planta (DCP). Mediu-se a temperatura da folha aos 176 DAT, com termômetro de infravermelho e o potencial da água na folha antes do amanhecer falv, aos 59, 96 e 110 DAT, com câmara de pressão. O número de frutos (NF), a massa fresca de frutos por planta (MFFP) e a massa total de frutos por planta (MTFP) foram quantificados. Calculou-se a taxa de incremento de crescimento (TMICP) e do diâmetro do caule da planta (TMIDCP). Os dados foram submetidos à análise de variância para testar a significância das lâminas de irrigação (plena e com restrição) diferenciadas na E1 e E2 e, quando significativo, determinou-se a tendência por meio de análise de regressão. Foram instaladas sondas de dissipação térmica - SDT em 29 plantas com 8 meses de idade. A equação para estimativa do fluxo de seiva foi calibrada tendo como padrão medidas lisimétricas em uma planta. Os volumes de irrigação totais aplicados variaram de 160 (E1L40) a 362 L planta-1 (L100), num ciclo de 208 DAT. Houve efeito significativo da irrigação plena e com déficit aplicado a partir da fase vegetativa e de floração sobre a AP, DCP, TMICP, TMIDCP, folha antes do amanhecer, temperatura média da folha, NFP, sendo o modelo linear o que melhor se ajustou. A MMFP não variou com o déficit, nem com as épocas de inicialização. O déficit de irrigação 40% Etc imposto na E2 permitiu economia de até 49,8% de água no ciclo de 208 DAT. O déficit de irrigação afetou a produção da pimenta, mas o mesmo não foi observado para a sua qualidade física. Há maior influência do déficit de irrigação na relação hídrica da pimenteira quando aplicado a partir da fase de floração. O método da SDT, com ajuste da calibração original, correção das diferenças térmicas naturais e uma correta amostragem na determinação da área do xilema, é eficaz na avaliação da transpiração de pimenta Tabasco. Palavras-chave: Capsicum frutescens; Restrição de irrigação; Estresse hídrico; Sonda de dissipação de calor / The deficit irrigation has been used as one of the most advantageous strategies for saving water, reducing costs witch irrigation and the opportunity cost of water. The main objective of this study was to evaluate the effect of full irrigation and with deficits in two different periods in plant growth, water relations, production parameters and quality of pepper cv. Tabasco (Capsicum frutescens). As a secondary objective method to evaluate the thermal dissipation probe (SDT) in the estimation of transpiration pepper \'Tabasco\'. The experiment was conducted in a greenhouse at the Biosystems Engineering Department at the Escola Superior de Agricultura \"Luiz de Queiroz\" - ESALQ-USP, Piracicaba-SP, from September 2009 to August 2010. The experiment was conduced as randomized block design with four replications and irrigation levels of 40, 60, 80 and 100% of crop evapotranspiration (Etc), beginning 39 days after transplanting - DAT (E1) and 59 DAT (E2) . The plant height (PH), fruit length (FL), fruit diameter (FD) and the stem diameter (SD) were measured. Leaf temperature was measured upon 176 DAT, with infrared thermometer, also the pre-dawn leaf water f predawn, at 59, 96 and 110 DAT, pressure chamber was measured. The fruit number (FN), average mass fruit per plant (AMFP) and the total mass of fruits per plant were (TMFP) are quantified. The rate growth increment (RGI) and the plant stem diameter (PSD), considering the current and previous evaluation were calculated. Data were subjected to analysis of variance to test the significance of irrigation (full and restricted) differentiated in E1 and E2, and when significant, the trend was determined by regression analysis. A heat dissipation probe - HDP were installed in 29 plants 8 months old. The equation to estimate the sap flow was calibrated using a lysimeter as a standard measure in a plant. The total volume of irrigation applied ranged from 160 (E1L40) to 362 L plant-1 (L100), in a cycle of 208 days from transplanting. There was a significant deficit and full irrigation started at vegetative stage and flowering on the PH, SD, RGI, PSD, f pre-dawn, average leaf temperature, FN been the linear model that best fitted. The average fruit plant mass did not vary with deficit neither with beginning time. The deficit irrigation 40% Etc in E2 allowed savings of up to 49.8% water consumption in a cycle 208 DAT. The deficit irrigation affected the pepper production witch was not observed for the physical quality of the fruits. There is greater influence of deficit irrigation on pepper water relation when applied from the flowering stage. It can be concluded the method of HDP, adjusting the original calibration, correction of the natural thermal differences and a correct sampling in determining the area of the xylem, is effective in evaluating the transpiration of pepper Tabasco.

O objetivo principal desta pesquisa foi desenvolver um modelo de simulação dinâmica para a valoração ecológica de serviços ecossistêmicos hídricos, baseado em princípios de Dinâmica de Sistemas com o auxílio do software STELLA. A hipótese básica adotada foi que: a valoração ecológica dos serviços ecossistêmicos deve contar com uma ferramenta integradora dos elementos envolvidos na modelagem ecológica como requisito básico para compreensão da dinâmica ecossistêmica que gera fluxos de serviços ecossistêmicos hídricos (SEH). Os SEH são os benefícios gerados a partir das complexas interações, entre os componentes do ciclo hidrológico e que tem a ver com a preservação tanto em quantidade como em qualidade da oferta de água. O modelo foi desenvolvido, através de uma análise sistêmica dos processos ecossistêmicos compreendidos dentro do ciclo hidrológico, foram determinadas as principais variáveis e suas respectivas inter-relações, de acordo com a estrutura desse ciclo. Essa estrutura foi representada no diagrama causal, que serviu de base à elaboração do diagrama de estoque e fluxo, por meio do qual se estabeleceu o modelo matemático que permitiu efetuar a simulação numérica. A escala temporal de aplicação do modelo é diária e foram efetuadas 365 simulações, o que permitiu estimar fluxos anuais de SEH. O modelo foi parametrizado na sub-bacia das Posses, Município de Extrema, Minas Gerais; aqui esta sendo implementado o primeiro projeto piloto, baseado na relação floresta-água, de pagamentos por SEH do Brasil. Foram examinados 36 cenários, com o objetivo de avaliar o impacto que têm variáveis como: textura de solo, altura da vegetação, índice de área foliar e profundidade do lençol freático na geração dos fluxos de SEH em água na superfície, infiltrada e armazenada; porém foi avaliado como estes incidem de forma quantitativa na manutenção da oferta de água disponível. O modelo desenvolvido permitiu aumentar a compreensão dos fluxos de SEH, o que representa uma ferramenta de elevado potencial de aplicação nas fases de definição de âmbito, de avaliação de alternativas e monitoramento de esquemas de pagamentos por SEH no Brasil. / The main objective of this research was to develop a dynamic simulation model to valuate ecological hydrological ecosystem services. The principles of System Dynamics were applied using the software STELLA. A basic hypothesis was adopted: ecological valuation of ecosystem services must use a tool to integer all the elements involved in ecological modeling, as a basic requirement to understand the ecosystem dynamic that generates water fluxes as hydrological ecosystems services (HES). HES are the benefits generated from complex interactions that occur within the hydrologic cycle; those interactions deals with the preservation of available water, in quantity and quality. The model was developed after a systemic analysis of the ecosystem processes involved in the hydrologic cycle; pointing out the main variables and the interrelations that constitute it. Then, the structure of those relations was represented in a causal diagram; becoming the base to built up the stocks and flows diagram. This later diagram established the mathematical model that allowed the numerical simulation. The time step of the model is one day during 365 days, estimating annual water HES. The model was parameterized at Poses sub-watershed, Extrema County, Minas Gerais. In Extrema was established the first payment for HES project in Brazil, based on the relationship between forest land and water availability. Thirty six scenarios were studied considering surface, infiltrated and stored water services fluxes; affecting the availability of water. The objective of those scenarios was to evaluate the impact that variables such as: soil texture, vegetation height, leaf area index and groundwater depth have on water flux generation. The model developed enables to increase the understanding of HES fluxes, becoming a tool with a broad spectrum of applications during the stages of assessing and scoping alternatives for payment of environmental services and to monitor HES programs in Brazil.