No description available.

[pt] A velocidade da onda de pulso (VOP) tem sido identificada como o padrão-ouro para avaliação da rigidez arterial e, recentemente, vem sendo reconhecida como um importante indicador no diagnóstico e tratamento de doenças cardiovasculares. Atualmente, já existem dispositivos comerciais capazes de efetuar a medição da VOP, entretanto, ainda exigem um investimento financeiro significativo e alguns requerem um treinamento especializado para seu correto uso. Os, transdutores de pressão atuais são majoritariamente baseados em sensores piezoresistivos, piezoelétricos e capacitivos. Entretanto, pesquisas recentes demostraram que transdutores de pressão que utilizam sensores magnéticos baseados na magnetoimpedância gigante (GMI) apresentam elevada sensibilidade. Tendo em vista que a VOP é um importante indicador do risco de distúrbios cardiovasculares, e considerando os potenciais beneficios dos sensores GMI em relação às demais alternativas, esta tese de doutorado buscou utilizar-se destes elementos sensores a fim de desenvolver um sistema de medição portátil, não-invasivo, de baixo custo, acessível e simples de usar, capaz de efetuar a medição da VOP. Neste intuito, foram desenvolvidos transdutores de alta sensibilidade, baseados nas características de fase da impedância de sensores de Magnetoimpedância Gigante, destinados à medição da velocidade da onda de pulso arterial. A fim de se otimizar as características de desempenho dos transdutores, foram realizadas avaliações teórico-computacionais dos transdutores na configuração em malha aberta e fechada, bem como ensaios experimentais dos protótipos projetados. As caracterizações e ensaios experimentais realizados com o transdutor de pressão em malha aberta resultaram em uma sensibilidade de 59,6 mV/kPa, e resolução de 192,8 Pa para uma média de 30 amostras, na banda de passagem de 1000 Hz. Por outro lado, a configuração em malha fechada apresentou uma sensibilidade de 54,2 mV/kPa, e resolução de 206,0 Pa para uma média de 30 amostras, na banda de passagem de 32 Hz. Tendo em vista os valores de sensibilidade e resolução obtidos, propõe-se empregar o sistema de transdução de pressão que incorpora uma câmara incompressível para amplificação mecânica, na medição de ondas de pulso arterial. Neste protótipo, uma pequena membrana semirrígida localizada na superfície da câmara incompressível é posicionada sobre a superfície da pele, próxima à artéria de interesse. Deste modo, pequenas mudanças de pressão na superfície da pele, causadas pela onda de pulso arterial, provocam uma variação do campo magnético sobre o elemento sensor. Por outra parte, devido à alta sensibilidade apresentada pelo transdutor magnetico (magnetômetro GMI) na configuração de malha aberta (0,2 mV/nT) e de malha fechada (0,19 mV/nT), estes foram usados para medir diretamente a forma de onda do pulso arterial, sem utilizar uma câmara incompressível para transdução mecânica. Nesta medição, considerando a adequada resolução espacial para as demandas anatômicas, utiliza-se um pequeno marcador magnético, envolto por uma fita adesiva hipoalergênica e flexível, aderida á região da pele sobre a artéria de interesse, e aproxima-se o sensor magnético GMI da superfície da pele onde o marcador foi colocado. Finalmente, as configurações propostas foram analisadas e comparadas, a fim de se identificar aquela com melhor desempenho, a qual foi utilizada para medição da VOP. Como o estudo envolve o registro da onda de pulso em participantes da pesquisa, o projeto foi submetido à apreciação e aprovado pela Comissão da Câmara de Ética em Pesquisa da Pontifícia Universidade Católica do Rio de Janeiro (PUC-Rio) 045/2020 – Protocolo 83/2020. Espera-se que o dispositivo desenvolvido contribua para o avanço tecnológico do ferramental utilizado no setor da saúde. / [en] Pulse wave velocity (PWV) is considered the gold standard for assessing arterial stiffness and recently, it has been recognized as an important indicator in the diagnosis and treatment of cardiovascular disease. Currently, there are commercial devices capable of measuring PWV, however, significant investments are required and some devices requires specialized training for their correct use. Conventional pressure-sensing devices are mainly based on piezoresistive, piezoelectric and capacitive sensors. Recent investigations, however, show that pressure transducer using magnetic sensors based on the giant Magnetoimpedance (GMI) present high-sensitivity. Considering that, PWV is a significant risk factor for future cardiovascular disease and in view of some of the advantages of GMI sensors in relation to another sensing technologies, this doctoral thesis aims to develop a portable measurement system, non-invasive, low-cost, accessible and simple to use, capable of measuring PWV. For this purpose, we have developed a high-sensitivity transducers based on the impedance phase characteristics of GMI sensors, for measuring the arterial pulse wave velocity. In order to improve the performance characteristics of the transducers, computational and theoretical analysis in open and closed loop configuration were performed. The characterizations and experimental tests performed with the open-loop pressure transducer resulted in a sensitivity of 59.6 mV/kPa, and resolution of 192.8 Pa for an average of 30 samples, in the 1000 Hz passband. On the other hand, the closed-loop configuration presented a sensitivity of 54.2 mV/kPa, and a resolution of 206.0 Pa for an average of 30 samples, in the 32 Hz passband. In view of the considerable sensitivity and resolution obtained, it is proposed to employ a pressure transduction system that incorporates an incompressible chamber for mechanical amplification, in the measurement of arterial pulse waves. In this prototype, a small semi-rigid membrane located on the surface of the incompressible chamber is positioned over the surface of the skin, close to the artery of interest. In this way, small pressure changes on the skin surface, caused by the arterial pulse wave, cause a variation of the magnetic field on the sensing element. On the other hand, due to the high sensitivity presented by the magnetic transducer (GMI magnetometer) in the open-loop (0.2 mV/nT) and closed-loop (0.19 mV/nT) configurations, they were used to measure the shape pulse waveform without using an incompressible chamber for mechanical transduction. In this test, considering the adequate spatial resolution for the anatomical demands, a small magnetic marker is used, the magnetic marker is attached to the skin region over the artery of interest, and the GMI magnetic sensor is approached near the marker placed of the skin surface. Finally, the proposed configurations were analyzed and compared in order to identify the one with the best performance, which was used to measure PWV. As the study involves recording the pulse wave in research participants, the project was submitted for consideration and approved by the Research Ethics Committee of the Pontifical Catholic University of Rio de Janeiro (PUC-Rio) 045/2020 – Protocol 83/2020. It is expected that the device developed will contribute to the technological advancement of the tools used in the health sector.

No description available.

Submitted Ph.D. thesis is concerning the application and interpretation of seismic surface waves in a broad range of frequencies and scales. Using surface waves as a supplement to the methods dealing with body waves seems to be worth the effort. Surface wave interpretation can be used to obtain new information about the studied medium and simultaneously it can overcome, in some cases, the limitations of other seismic techniques. Moreover, surface waves are usually present on measured records and hence for its usage it is not necessary to modify the standard measuring procedures. One of the results of this thesis is an original algorithm for dispersive waveform calculation. The program works in an arbitrary range of frequencies and scales. The input parameter for the calculation is the dispersion curve. In this point the algorithm differs from all other approaches used so far. Algorithm is based on a summation of frequency components with shifts corresponding to the velocity dispersion and distance. The resulting waveform only contains an individual dispersive wave of the selected mode, thus being particularly suitable for testing of methodologies for dispersive wave analysis. The algorithm was implemented into the program DISECA. Furthermore, a new procedure was designed to calculate the dispersion...

Dados sobre a avaliação não invasiva da rigidez vascular e suas relações com variáveis de risco cardiovascular são escassos em jovens. Objetiva avaliar a relação entre a velocidade de onda de pulso (VOP) e a pressão arterial (PA), variáveis antropométricas, metabólicas, inflamatórias e de disfunção endotelial em indivíduos adultos jovens. Foram estudados 96 indivíduos (51 homens) do Estudo do Rio de Janeiro, em duas avaliações, A1 e A2, com intervalo de 17,691,58 anos (16 a 21 anos). Em A1 foram avaliados em suas escolas (10-15 anos - média 12,421,47 anos) e em A2 foram novamente avaliados em nível ambulatorial (26-35 anos - média 30,091,92 anos). Em A1 foram obtidos pressão arterial (PA) e índice de massa corporal (IMC). Em A2 foram obtidos a velocidade da onda de pulso (VOP)-método Complior, PA, IMC, circunferência abdominal (CA), glicose, perfil lipídico, leptina, insulina, adiponectina, o índice de resistência à insulina HOMA-IR, proteína C-Reativa ultrassensível (PCRus) e as moléculas de adesão E-selectina, Vascular Cell Adhesion Molecule-1(VCAM-1) e Intercellular Adhesion Molecule-1 (ICAM-1). Foram obtidos, ainda, a variação da PA e do IMC entre as 2 avaliações. Em A2 os indivíduos foram estratificados segundo o tercil da VOP para cada sexo. Como resultados temos: 1) Os grupos foram constituídos da seguinte forma: Tercil 1:homens com VOP < 8,69 m/s e mulheres com VOP < 7,66 m/s; Tercil 2: homens com VOP &#8805; 8,69 m/s e < 9,65m/s e mulheres com VOP &#8805; 7,66 m/s e < 8,31m/s;Tercil 3:homens com VOP &#8805; 9,65 m/s e mulheres com VOP &#8805; 8,31 m/s. 2) O grupo com maior tercil de VOP mostrou maiores médias de PA sistólica (PAS) (p=0,005), PA diastólica (PAD) (p=0,007), PA média (PAM) (p=0,004), variação da PAD (p=0,032), variação da PAM (p=0,003), IMC (p=0,046), variação do IMC (p=0,020), insulina (p=0,019), HOMA-IR (p=0,021), E-selectina (p=0,032) e menores médias de adiponectina (p=0,016), além de maiores prevalências de diabetes mellitus/intolerância à glicose (p=0,022) e hiperinsulinemia (p=0,038); 3) Houve correlação significativa e positiva da VOP com PAS (p<0,001), PAD (p<0,001), PP (p=0,048) e PAM (p<0,001) de A2, com a variação da pressão arterial (PAS, PAD e PAM) (p<0,001) entre as duas avaliações, com o IMC de A2 (p=0,005) e com a variação do IMC (p<0,001) entre as duas avaliações, com CA (p=0,001), LDLcolesterol (p=0,049) e E-selectina (p<0,001) e correlação negativa com HDLcolesterol (p<0,001) e adiponectina (p<0,001); 4)Em modelo de regressão múltipla, após ajuste do HDL-colesterol, LDLcolesterol e adiponectina para sexo, idade, IMC e PAM, apenas o sexo masculino e a PAM mantiveram correlação significativa com a VOP. A VOP em adultos jovens mostrou relação significativa com variáveis de risco cardiovascular, destacando-se o sexo masculino e a PAM como importantes variáveis no seu determinismo. Os achados sugerem que a medida da VOP pode ser útil para a identificação do acometimento vascular nessa faixa etária. / Data on non-invasive evaluation of vascular stiffness in the young and its relationship with cardiovascular (CV) risk variables are scarce. Objective to assess the relationship between pulse wave velocity (PWV) and blood pressure (BP), anthropometric, metabolic, inflammatory and endothelial dysfunction variables in young adults. Ninety-six individuals (51 males) from The Rio de Janeiro Study cohort were studied in two evaluations, A1 and A2, with an interval of 17.69 1.58 years (16-21 years). In A 1 they were evaluated at their schools (10-15 years average 12.42 1.47 years) and in A2 they were all re-evaluated as outpatients (26-35 years - average 30.09 1.92 years). In A1 BP and body mass index (BMI) were obtained. In A2 pulse wave velocity (PWV) by Complior method, BP, BMI, waist circumference (WC), glucose, lipid profile, leptin, insulin, adiponectin, the HOMA-IR insulin resistance index, high sensitive C-Reactive protein (CRPhs) and E-selectin, Vascular Cell Adhesion Molecule 1 (VCAM-1) and Intercellular Adhesion Molecule 1 (ICAM-1) adhesion molecules were obtained. The BP and BMI variation over the time interval between the two evaluations were also obtained. Subjects were stratified according to tertile of PWV for each sex in A2. As results: 1) The groups were constituted as follows: Tertile 1: males with PWV <8.69 m/s and females with PWV <7.66 m/s; Tertile 2: males with PWV &#8805; 8.69 m/s and <9.65 m/s and females with PWV &#8805; 7.66 m/s and <8.31 m/s; Tertile 3: males with PWV &#8805; 9.65 m/s and females with PWV &#8805; 8.31 m/s 2) The group with the highest PWV tertile showed higher values of systolic BP (SBP) (p=0.005), diastolic BP (DBP) (p=0.007), mean BP (MBP) (p=0,004), DBP variation (p=0,032), MBP variation (p=0.033), BMI (p=0.046), BMI variation (p=0.020), insulin (p=0.019), HOMA-IR (p=0.021), E-Selectin (p=0.032) and lower values of adiponectin (p=0.016), besides higher prevalence of diabetes mellitus/glucose intolerance (p=0.022) and hyperinsulinemia (p=0.038); 3) There were a significant positive correlation of PWV with SBP (p<0,001), DBP (p<0,001), PP (p=0,048) and MBP (p<0,001) from A2, variation in blood pressure (SBP, DBP, and MBP) (p<0,001) between the two assessments, BMI (p=0.005) and BMI variation between the two evaluations (p<0,001), WC (p=0.001), LDL-cholesterol (0.049), and E-selectin (p<0,001) and negative correlation with HDL-cholesterol (p<0,001) and adiponectin (p<0,001); 4) In the multiple regression model, HDL-cholesterol, LDL-cholesterol and adiponectin lost statistical significance after adjustment for sex, age, BMI and MBP, only the male gender and MBP remained significantly correlated with PWV. PWV in young adults showed a significant association with CV risk variables, highlighting the male gender and MBP as important variables in its determining. The findings suggest that measurement of PWV can be useful for the identification of vascular impairment in this age group.

Dados sobre a avaliação não invasiva da rigidez vascular e suas relações com variáveis de risco cardiovascular são escassos em jovens. Objetiva avaliar a relação entre a velocidade de onda de pulso (VOP) e a pressão arterial (PA), variáveis antropométricas, metabólicas, inflamatórias e de disfunção endotelial em indivíduos adultos jovens. Foram estudados 96 indivíduos (51 homens) do Estudo do Rio de Janeiro, em duas avaliações, A1 e A2, com intervalo de 17,691,58 anos (16 a 21 anos). Em A1 foram avaliados em suas escolas (10-15 anos - média 12,421,47 anos) e em A2 foram novamente avaliados em nível ambulatorial (26-35 anos - média 30,091,92 anos). Em A1 foram obtidos pressão arterial (PA) e índice de massa corporal (IMC). Em A2 foram obtidos a velocidade da onda de pulso (VOP)-método Complior, PA, IMC, circunferência abdominal (CA), glicose, perfil lipídico, leptina, insulina, adiponectina, o índice de resistência à insulina HOMA-IR, proteína C-Reativa ultrassensível (PCRus) e as moléculas de adesão E-selectina, Vascular Cell Adhesion Molecule-1(VCAM-1) e Intercellular Adhesion Molecule-1 (ICAM-1). Foram obtidos, ainda, a variação da PA e do IMC entre as 2 avaliações. Em A2 os indivíduos foram estratificados segundo o tercil da VOP para cada sexo. Como resultados temos: 1) Os grupos foram constituídos da seguinte forma: Tercil 1:homens com VOP < 8,69 m/s e mulheres com VOP < 7,66 m/s; Tercil 2: homens com VOP &#8805; 8,69 m/s e < 9,65m/s e mulheres com VOP &#8805; 7,66 m/s e < 8,31m/s;Tercil 3:homens com VOP &#8805; 9,65 m/s e mulheres com VOP &#8805; 8,31 m/s. 2) O grupo com maior tercil de VOP mostrou maiores médias de PA sistólica (PAS) (p=0,005), PA diastólica (PAD) (p=0,007), PA média (PAM) (p=0,004), variação da PAD (p=0,032), variação da PAM (p=0,003), IMC (p=0,046), variação do IMC (p=0,020), insulina (p=0,019), HOMA-IR (p=0,021), E-selectina (p=0,032) e menores médias de adiponectina (p=0,016), além de maiores prevalências de diabetes mellitus/intolerância à glicose (p=0,022) e hiperinsulinemia (p=0,038); 3) Houve correlação significativa e positiva da VOP com PAS (p<0,001), PAD (p<0,001), PP (p=0,048) e PAM (p<0,001) de A2, com a variação da pressão arterial (PAS, PAD e PAM) (p<0,001) entre as duas avaliações, com o IMC de A2 (p=0,005) e com a variação do IMC (p<0,001) entre as duas avaliações, com CA (p=0,001), LDLcolesterol (p=0,049) e E-selectina (p<0,001) e correlação negativa com HDLcolesterol (p<0,001) e adiponectina (p<0,001); 4)Em modelo de regressão múltipla, após ajuste do HDL-colesterol, LDLcolesterol e adiponectina para sexo, idade, IMC e PAM, apenas o sexo masculino e a PAM mantiveram correlação significativa com a VOP. A VOP em adultos jovens mostrou relação significativa com variáveis de risco cardiovascular, destacando-se o sexo masculino e a PAM como importantes variáveis no seu determinismo. Os achados sugerem que a medida da VOP pode ser útil para a identificação do acometimento vascular nessa faixa etária. / Data on non-invasive evaluation of vascular stiffness in the young and its relationship with cardiovascular (CV) risk variables are scarce. Objective to assess the relationship between pulse wave velocity (PWV) and blood pressure (BP), anthropometric, metabolic, inflammatory and endothelial dysfunction variables in young adults. Ninety-six individuals (51 males) from The Rio de Janeiro Study cohort were studied in two evaluations, A1 and A2, with an interval of 17.69 1.58 years (16-21 years). In A 1 they were evaluated at their schools (10-15 years average 12.42 1.47 years) and in A2 they were all re-evaluated as outpatients (26-35 years - average 30.09 1.92 years). In A1 BP and body mass index (BMI) were obtained. In A2 pulse wave velocity (PWV) by Complior method, BP, BMI, waist circumference (WC), glucose, lipid profile, leptin, insulin, adiponectin, the HOMA-IR insulin resistance index, high sensitive C-Reactive protein (CRPhs) and E-selectin, Vascular Cell Adhesion Molecule 1 (VCAM-1) and Intercellular Adhesion Molecule 1 (ICAM-1) adhesion molecules were obtained. The BP and BMI variation over the time interval between the two evaluations were also obtained. Subjects were stratified according to tertile of PWV for each sex in A2. As results: 1) The groups were constituted as follows: Tertile 1: males with PWV <8.69 m/s and females with PWV <7.66 m/s; Tertile 2: males with PWV &#8805; 8.69 m/s and <9.65 m/s and females with PWV &#8805; 7.66 m/s and <8.31 m/s; Tertile 3: males with PWV &#8805; 9.65 m/s and females with PWV &#8805; 8.31 m/s 2) The group with the highest PWV tertile showed higher values of systolic BP (SBP) (p=0.005), diastolic BP (DBP) (p=0.007), mean BP (MBP) (p=0,004), DBP variation (p=0,032), MBP variation (p=0.033), BMI (p=0.046), BMI variation (p=0.020), insulin (p=0.019), HOMA-IR (p=0.021), E-Selectin (p=0.032) and lower values of adiponectin (p=0.016), besides higher prevalence of diabetes mellitus/glucose intolerance (p=0.022) and hyperinsulinemia (p=0.038); 3) There were a significant positive correlation of PWV with SBP (p<0,001), DBP (p<0,001), PP (p=0,048) and MBP (p<0,001) from A2, variation in blood pressure (SBP, DBP, and MBP) (p<0,001) between the two assessments, BMI (p=0.005) and BMI variation between the two evaluations (p<0,001), WC (p=0.001), LDL-cholesterol (0.049), and E-selectin (p<0,001) and negative correlation with HDL-cholesterol (p<0,001) and adiponectin (p<0,001); 4) In the multiple regression model, HDL-cholesterol, LDL-cholesterol and adiponectin lost statistical significance after adjustment for sex, age, BMI and MBP, only the male gender and MBP remained significantly correlated with PWV. PWV in young adults showed a significant association with CV risk variables, highlighting the male gender and MBP as important variables in its determining. The findings suggest that measurement of PWV can be useful for the identification of vascular impairment in this age group.

Ce travail de thèse est axé sur le domaine du traitement d’images biomédicales. L’objectif de notre étude est l’estimation des paramètres traduisant les propriétés mécaniques de l’artère carotide in vivo en imagerie échographique, dans une optique de détection précoce des pathologies cardiovasculaires. L’étude des comportements dynamiques de l’artère pour le dépistage précoce de l’athérosclérose constitue à ce jour une piste privilégiée. Cependant, malgré les avancées récentes, l’estimation du mouvement de la paroi carotidienne reste toujours difficile, notamment dans la direction longitudinale (direction parallèle au vaisseau). L’élaboration d’une méthode innovante permettant d’étudier le mouvement de la paroi carotidienne constitue la principale motivation de ce travail de thèse. Les trois contributions principales proposées dans ce travail sont i) le développement, la validation, et l’évaluation clinique d’une méthode originale d’estimation de mouvement 2D adaptée au mouvement de la paroi carotidienne, ii) la validation en simulation, et expérimentale de l’extension à la 3D de la méthode d’estimation proposée, et iii) l’évaluation expérimentale de la méthode proposée, en imagerie ultrasonore ultra-rapide, dans le cadre de l’estimation locale de la vitesse de l’onde de pouls. Nous proposons une méthode d’estimation de mouvement combinant un marquage ultrasonore dans la direction latérale, et un estimateur de mouvement basé sur la phase des images ultrasonores. Le marquage ultrasonore est réalisé par l’intermédiaire d’oscillations transverses. Nous proposons deux approches différentes pour introduire ces oscillations transverses, une approche classique utilisant une fonction de pondération spécifique, et une approche originale par filtrage permettant de contrôler de manière optimale leurs formations. L’estimateur de mouvement proposé utilise les phases analytiques des images radiofréquences, extraites par l’approche de Hahn. Ce travail de thèse montre que la méthode proposée permet une estimation de mouvement plus précise dans la direction longitudinale, et plus généralement dans les directions perpendiculaires au faisceau ultrasonore, que celle obtenue avec d’autres méthodes plus traditionnelles. De plus, l’évaluation expérimentale de la méthode sur des séquences d’images ultrasonores ultra-rapides issues de fantômes de carotide, a permis l’estimation locale de la vitesse de propagation de l’onde de pouls, la mise en évidence de la propagation d’un mouvement longitudinal et enfin l’estimation du module de Young des vaisseaux. / This work focuses on the processing of biomedical images. The aim of our study is to estimate the mechanical properties of the carotid artery in vivo using ultrasound imaging, in order to detect cardiovascular diseases at an early stage. Over the last decade, researchers have shown interest in studying artery wall motion, especially the motion of the carotid intima-media complex in order to demonstrate its significance as a marker of Atherosclerosis. However, despite recent progress, motion estimation of the carotid wall is still difficult, particularly in the longitudinal direction (direction parallel to the probe). The development of an innovative method for studying the movement of the carotid artery wall is the main motivation of this thesis. The three main contributions proposed in this work are i) the development, the validation, and the clinical evaluation of a novel method for 2D motion estimation of the carotid wall, ii) the development, the simulation and the experimental validation of the 3D extension of the estimation method proposed, and iii) the experimental evaluation of the 2D proposed method in ultra-fast imaging, for the estimation of the local pulse wave velocity. We propose a motion estimation method combining tagging of the ultrasound images, and a motion estimator based on the phase of the ultrasound images. The ultrasonic tagging is produced by means of transverse oscillations. We present two different approaches to introduce these transverses oscillations, a classic approach using a specific apodization function and a new approach based on filtering. The proposed motion estimator uses the 2D analytical phase of RF images using the Hahn approach. This thesis work shows that, compared with conventional methods, the proposed approach provides more accurate motion estimation in the longitudinal direction, and more generally in directions perpendicular to the beam axis. Also, the experimental evaluation of our method on ultra-fast images sequences from carotid phantom was used to validate our method regarding the estimation of the pulse wave velocity, the Young’s modulus of the vessels wall, and the propagation of a longitudinal movement.

Cardiovascular disease varies by ethnicity in the UK. South Asians (SA) have higher coronary heart disease (CHD) and diabetes prevalence, while African-Caribbeans (AfC) have greater stroke, but intriguingly lower CHD rates despite higher blood pressures and diabetes risk than Europeans. Conventional risk factors do not fully explain such differences. This cross-sectional study tested the hypothesis that the hormones, vitamin D measured as 25(OH)D and aldosterone, would be independently associated with intermediate cardiovascular outcome markers in these ethnic groups. Community-dwelling men 40-80 years old (AfC: n=67, 55±10yr; SA: n=68, 55±10yr; European: n=63, 57±8yr) were sampled from Greater Manchester’s multi-ethnic population. The intermediate markers examined were aortic pulse wave velocity (aPWV), left ventricular (LV) mass and function, and carotid intima media thickness (CIMT), measured non-invasively by ultrasound, and hemodynamic profiling methods (the Arteriograph) in the total sample and by magnetic resonance imaging (MRI) in a subsample of 50. Adjusted for age, systolic blood pressure and diabetes, mean(SE) aPWV by the Arteriograph, was 0.5(0.2) m/s higher in SA than AfC and Europeans (p=0.01), which paralleled known cross-ethnic CHD risk differences in the UK. By MRI, aPWV along the descending aorta in SA was 0.7(0.3) and 0.8(0.3) m/s higher than that in AfC and Europeans, but aPWV along the aortic arch was not significantly different. Unlike aldosterone, 25(OH)D was independently and inversely correlated with aPWV (unstandardised B(SE)=-0.013[0.004] m/s, p<0.001), and partly explained the ethnic variation in aPWV. Similar inverse correlations were found between 25(OH)D and LV concentricity measured by echocardiography and MRI. Compared to Europeans, SA and AfC, had 21(3) and 14(3) nmol/L lower mean(SE) 25(OH)D, respectively (p<0.01). Mean(SE) of relative wall thickness, an index of LV concentricity by echocardiography, was 0.05(0.01) higher in SA and AfC than Europeans. Lower 25(OH)D levels were also associated with higher myocardial deformation rates measured by MRI myocardial tagging (n=50), supporting previous animal experimental evidence. A one standard deviation (SD) decrease in 25(OH)D was associated with a 0.38 SD increase in absolute systolic strain rate (p=0.003) and 0.22 SD rise in diastolic strain rate (p=0.04). Right and left CIMT showed different relations with 25(OH)D and aldosterone. Left-right CIMT differences varied by ethnicity and were related to SA ethnicity and aldosterone levels. Two related technical studies investigated the relatively new method of hemodynamic profiling, the Arteriograph, used here. The results suggested a standardisation method of aortic length estimation for purely central aPWV, which significantly improved aPWV agreement between the Arteriograph and MRI (reference method here), and was used for calibrating the Arteriograph aPWV in the above-mentioned results for the total sample. Future well-designed trials are necessary to investigate any cause-effect relationship between vitamin D deficiency and the unfavourable cardiovascular intermediate outcomes found here in a cross-sectional design and multi-ethnic background.

Mankind is facing the problem due to earthquake hazard since prehistoric times. Many of the developed and developing countries are under constant threats from earthquakes hazards. Theories of plate tectonics and engineering seismology have helped to understand earthquakes and also to predicate earthquake hazards on a regional scale. However, the regional scale hazard mapping in terms of seismic zonation has been not fully implemented in many of the developing countries like India. Agglomerations of large population in the Indian cities and poor constructions have raised the risk due to various possible seismic hazards. First and foremost step towards hazard reduction is estimation of the seismic hazards in regional scale. Objective of this study is to estimate the seismic hazard parameters for Lucknow, a part of Indo-Gangetic Basin (IGB) and develop regional scale microzonation map. Lucknow is a highly populated city which is located close to the active seismic belt of Himalaya. This belt came into existence during the Cenozoic era (40-50 million years ago) and is a constant source of seismic threats. Many of the devastating earthquakes which have happened since prehistoric times such as 1255 Nepal, 1555 Srinagar, 1737 Kolkata, 1803 Nepal, 1833 Kathmandu, 1897 Shillong, 1905 Kangra, 1934 Bihar-Nepal, 1950 Assam and 2005 Kashmir. Historic evidences show that many of these earthquakes had caused fatalities even up to 0.1 million. At present, in the light of building up strains and non-occurrence of a great event in between 1905 Kangra earthquake and 1934 Bihar-Nepal earthquake regions the stretch has been highlighted as central seismic gap. This location may have high potential of great earthquakes in the near future. Geodetic studies in these locations indicate a possible slip of 9.5 m which may cause an event of magnitude 8.7 on Richter scale in the central seismic gap. Lucknow, the capital of Uttar Pradesh has a population of 2.8 million as per Census 2011. It lies in ZONE III as per IS1893: 2002 and can be called as moderate seismic region. However, the city falls within 350 km radial distance from Main Boundary Thrust (MBT) and active regional seismic source of the Lucknow-Faizabad fault. Considering the ongoing seismicity of Himalayan region and the Lucknow-Faizabad fault, this city is under high seismic threat. Hence a comprehensive study of understanding the earthquake hazards on a regional scale for the Lucknow is needed. In this work the seismic microzonation of Lucknow has been attempted. The whole thesis is divided into 11 chapters. A detailed discussion on the importance of this study, seismicity of Lucknow, and methodology adopted for detailed seismic hazard assessment and microzonation are presented in first three chapters. Development of region specific Ground Motion Prediction Equation (GMPE) and seismic hazard estimation at bedrock level using highly ranked GMPEs are presented in Chapters 4 and 5 respectively. Subsurface lithology, measurement of dynamic soil properties and correlations are essential to assess region specific site effects and liquefaction potential. Discussion on the experimental studies, subsurface profiling using geotechnical and geophysical tests results and correlation between shear wave velocity (SWV) and standard penetration test (SPT) N values are presented in Chapter 6. Detailed shear wave velocity profiling with seismic site classification and ground response parameters considering multiple ground motion data are discussed in Chapters 7 and 8. Chapters 9 and 10 present the assessment of liquefaction potential and determination of hazard index with microzonation maps respectively. Conclusions derived from each chapter are presented in Chapter 11. A brief summary of the work is presented below: Attenuation relations or GMPEs are important component of any seismic hazard analysis which controls accurate prediction of the hazard values. Even though the Himalayas have experienced great earthquakes since ancient times, suitable GMPEs which are applicable for a wide range of distance and magnitude are limited. Most of the available regional GMPEs were developed considering limited recorded data and/or pure synthetic ground motion data. This chapter presents development of a regional GMPE considering both the recorded as well as synthetic ground motions. In total 14 earthquakes consisting of 10 events with recorded data and 4 historic events with Isoseismal maps are used for the same. Synthetic ground motions based on finite fault model have been generated at unavailable locations for recorded events and complete range distances for historic earthquakes. Model parameters for synthetic ground motion were arrived by detailed parametric study and from literatures. A concept of Apparent Stations (AS) has been used to generate synthetic ground motion in a wide range of distance as well as direction around the epicenter. Synthetic ground motion data is validated by comparing with available recorded data and peak ground acceleration (PGA) from Isoseismal maps. A new GMPE has been developed based on two step stratified regression procedure considering the combined dataset of recorded and synthetic ground motions. The new GMPE is validated by comparing with three recently recorded earthquakes events. GMPE proposed in this study is capable of predicting PGA values close to recorded data and spectral acceleration up to period of 2 seconds. Comparison of new GMPE with the recorded data of recent earthquakes shows a good matching of ground motion as well as response spectra. The new GMPE is applicable for wide range of earthquake magnitudes from 5 to 9 on Mw scale. Reduction of future earthquake hazard is possible if hazard values are predicted precisely. A detailed seismic hazard analysis is carried out in this study considering deterministic and probabilistic approaches. New seismotectonic map has been generated for Lucknow considering a radial distance of 350 km around the city centre, which also covers active Himalayan plate boundaries. Past earthquakes within the seismotectonic region have been collected from United State Geological Survey (USGS), Northern California Earthquake Data Centre (NCEDC), Indian Meteorological Department (IMD), Seismic Atlas of India and its Environs (SEISAT) etc. A total of 1831 events with all the magnitude range were obtained. Collected events were homogenized, declustered and filtered for Mw ≥ 4 events. A total of 496 events were found within the seismic study region. Well delineated seismic sources are compiled from SEISAT. Superimposing the earthquake catalogue on the source map, a seismotectonic map of Lucknow was generated. A total of 47 faults which have experienced earthquake magnitude of 4 and above are found which are used for seismic hazard analysis. Based on the distribution of earthquake events on the seismotectonic map, two regions have been identified. Region I which shows high density of seismic events in the area in and around of Main Boundary Thrust (MBT) and Region II which consists of area surrounding Lucknow with sparse distribution of earthquake events. Data completeness analysis and estimation of seismic parameter “a” and “b” are carried out separately for both the regions. Based on the analysis, available earthquake data is complete for a period of 80 years in both the regions. Using the complete data set, the regional recurrence relations have been developed. It shows a “b” value of 0.86 for region I and 0.9 for Region II which are found comparable with earlier studies. Maximum possible earthquake magnitude in each source has been estimated using observed magnitude and doubly truncated Gutenberg-Richter relation. The study area of Lucknow is divided into 0.015o x 0.015o grid size and PGA at each grid has been estimated by considering all sources and the three GMPEs. A Matlab code was generated for seismic hazard analysis and maximum PGA value at each grid point was determined and mapped. Deterministic seismic hazard analysis (DSHA) shows that maximum expected PGA values at bedrock level varies from 0.05g in the eastern part to 0.13g in the northern region. Response spectrum at city centre is also developed up to a period of 2 seconds. Further, Probabilistic seismic hazard analysis (PSHA) has been carried out and PGA values for 10 % and 2 % probability of exceedence in 50 years have been estimated and mapped. PSHA for 10 % probability shows PGA variation from 0.035g in the eastern parts to 0.07g in the western and northern parts of Lucknow. Similarly PSHA for 2 % probability of exceedence indicates PGA variation from 0.07g in the eastern parts while the northern parts are expecting PGA of 0.13g. Uniform hazard spectra are also developed for 2 % and 10 % probability for a period of up to 2 seconds. The seismic hazard analyses in this study show that the northern and western parts of Lucknow are more vulnerable when compared to other part. Bedrock hazard values completely change due to subsoil properties when it reaches the surface. A detailed geophysical and geotechnical investigation has been carried out for subsoil profiling and seismic site classification. The study area has been divided into grids of 2 km x 2 km and roughly one geophysical test using MASW (Multichannel Analysis Surface Wave) has been carried out in each grid and the shear wave velocity (SWV) profiles of subsoil layers are obtained. A total of 47 MASW tests have been carried out and which are uniformly distributed in Lucknow. In addition, 12 boreholes have also been drilled with necessary sampling and measurement of N-SPT values at 1.5 m interval till a depth of 30 m. Further, 11 more borelog reports are collected from the same agency hired for drilling the boreholes. Necessary laboratory tests are conducted on disturbed and undisturbed soil samples for soil classification and density measurement. Based on the subsoil informations obtained from these boreholes, two cross-sections up to a depth of 30 m have been generated. These cross-sections show the presence of silty sand in the top 10 m at most of the locations followed by clayey sand of low to medium compressibility till a depth of 30 m. In between the sand and clay traces of silt were also been found in many locations. In addition to these boreholes, 20 deeper boreholes (depth ≥150 m) are collected from Jal Nigam (Water Corporation) Lucknow, Government of Uttar Pradesh. Typical cross-section along the alignment of these deeper boreholes has been generated up to 150 m depth. This cross-section shows the presence of fine sand near Gomati while other locations are occupied by surface clayey sand. Also, the medium sand has been found in the western part of the city at a depth of 110 m which continues till 150 m depth. On careful examination of MASW and boreholes with N-SPT, 17 locations are found very close and SWV and N-SPT values are available up to 30 m depth. These SWV and N-SPT values are complied and used to develop correlations between SWV and N-SPT for sandy soil, clayey soil and all soil types. This correlation is the first correlation for IGB soil deposits considered measured data up to 30 m. The new correlation is verified graphically using normal consistency ratio and standard percentage error with respect to measured N-SPT and SWV. Further, SWV and N-SPT profiles are used Another important earthquake induced hazard is liquefaction. Even though many historic earthquakes caused liquefaction in India, very limited attempt has been made to map liquefaction potential in IGB. In this study, a detailed liquefaction analysis has been carried out for Lucknow a part of Ganga Basin to map liquefaction potential. Initially susceptibility of liquefaction for soil deposits has been assessed by comparing the grain size distribution curve obtained from laboratory tests with the range of grain size distribution for potentially liquefiable soils. Most of surface soil deposits in the study area are susceptible to liquefaction. At all the 23 borehole locations, measured N-SPT values are corrected for (a) Overburden Pressure (CN), (b) Hammer energy (CE), (c) Borehole diameter (CB), (d) presence or absence of liner (CS), (e) Rod length (CR) and (f) fines content (Cfines). Surface PGA values at each borehole locations are used to estimate Cyclic Stress Ratio (CSR). Corrected N-SPT values [(N1)60CS] are used to estimate Cyclic Resistance Ratio (CRR) at each layer. CSR and CRR values are used to estimate Factor of Safety (FOS) against liquefaction in each layer. Least factor safety values are indentified from each location and presented liquefaction factor of safety map for average and maximum amplified PGA values. These maps highlight that northern, western and central parts of Lucknow are very critical to critical against liquefaction while southern parts shows moderate to low critical area. The entire alignment of river Gomati falls in very critical to critical regions for liquefaction. Least FOS shows worst scenario and does not account thickness of liquefiable soil layers. Further, these FOS values are used to determine Liquefaction Potential Index (LPI) of each site and developed LPI map. Based on LPI map, the Gomati is found as high to very high liquefaction potential region. Southern and the central parts of Lucknow show low to moderate liquefaction potential while the northern and western Lucknow has moderate to high liquefaction potential. All possible seismic hazards maps for Lucknow have been combined to develop final microzonation map in terms of hazard index values. Hazard index maps are prepared by combining rock PGA map, site classification map in terms of shear wave velocity, amplification factor map, and FOS map and predominant period map by adopting Analytical Hierarchy Process (AHP). All these parameters have been given here in the order starting with maximum weight of 6 for PGA to lower weight of 1 for predominant frequency. Normalized weights of each parameter have been estimated. Depending upon the variation of each hazard parameter values, three to five ranks are assigned and the normalized ranks are calculated. Final hazard index values have been estimated by multiplying normalized ranks of each parameter with the normalized weights. Microzonation map has been generated by mapping hazard index values. Three maps were generated based on DSHA, PSHA for 2% and 10 % probability of exceedence in 50 years. Hazard index maps from DSHA and PSHA for 2 % probability show similar pattern. Higher hazard index were obtained in northern and western parts of Lucknow and lower values in others. The new microzonation maps can help in dividing the Lucknow into three parts as high area i.e. North western part, moderate hazard area i.e. central part and low hazard area which covers southern and eastern parts of Lucknow. This microzonation is different from the current seismic code where all area is lumped in one zone without detailed assessment of different earthquake hazard parameters. Finally this study brings out first region specific GMPE considering recorded and synthetic ground monitions for wide range of magnitudes and distances. Proposed GMPE can also be used in other part of the Himalayan region as it matches well with the highly ranked GMPEs. Detailed rock level PGA map has been generated for Lucknow considering DSHA and PSHA. A detailed geotechnical and geophysical experiments are carried out in Lucknow. These results are used to develop correction between SWV and N-SPT values for soil deposit in IGB and site classification maps for the study area. Amplification and liquefaction potential of Lucknow are estimated by considering multiple ground motions data to account different earthquake ground motion amplitude, duration and frequency, which is unique in the seismic microzonation study.

This Mater's thesis deals with the evaluation of pulse wave velocity using multi-channel whole-body impedance cardiography. Data were taken from the group of healthy volunteers whose impedance changes were measured during rest, respiratory maneuvers, tilt and stress exercise. The result of this measurement are values of peaks of pulse wave time shifts towards R-wave. The velocity values towards the thorax electrodes were recalculated on the basis of knowledge about the pulse wave time shifts and the distances from the heart to the scanned locations, which were measured using the arterial segment's lengths.