Lietuvos žemės ūkio universiteto Agroekologijos centro ekologinės gamybos ūkyje 2007-2008 m. atlikus fosforo ir kalio trąšų įtakos ekologiškai auginamiems žieminiams rugiams tyrimus nustatyta, kad didžiausias žieminių rugių derlingumas gautas tręšiant P60 fosforitmilčių norma. Didinant normą žieminių rugių derlingumas mažėjo. Lyginant fosforitmilčių normas tarpusavyje bei su netręštais žieminiais rugiais, esminių grūdų derlingumo skirtumų nenustatyta. Tręšimas fosforitmilčiais neturėjo esminės įtakos baltymų kiekiui ir tešlos kritimo skaičiui žieminių rugių grūduose bei natūriniam grūdų svoriui. Kalio trąšų formos ir normos neturėjo esminės įtakos žieminių rugių grūdų derlingumui, baltymų kiekiui ir tešlos kritimo skaičiui žieminių rugių grūduose bei natūriniam grūdų svoriui. / After having performed tests, in 2007-2008 year, the different fertilizers affect with the phosphorus and potassium to ecologically grown winter rye in the ecological manufacturing farm of Agro-ecology centre of Lithuanian University of Agriculture, it was identified that the highest yields of winter rye rate with phosphorus P60 fertilization. The yields declined increasing the rate of winter rye. It was identified that the phosphorus rate compared with each other and did’t have essential influence on the grain crop of winter rye. The fertilization with phosphorus not have essential influence on the protein content of the dough and drop in the number of winter rye grain and to the natural grain weight. The potassium fertilizer forms and rates did not have essential influence on the grain yields of winter rye and protein content and drop in the number of winter rye grain and to the natural grain weight.

Winter rye produces ice-modifying antifreeze proteins upon cold treatment. Two of these antifreeze proteins are members of the large, highly conserved, ??-1,3-endoglucanase family. This project was designed to identify glucanase genes that are expressed during cold acclimation, wounding, pathogen infection, drought or treatment with the phytohormones ethylene and MeJa. Additionally, a more detailed proteomic analysis was to be carried out to evaluate the glucanase content of the apoplast of cold-acclimated (CA) winter rye. Results of 2D SDS-PAGE analysis revealed that non-acclimated whole leaf protein extracts contain at least two ??-1,3-endoglucanses while CA whole leaf protein extracts contain at least three ??-1,3-endoglucanses. Subsequent 2D SDS-PAGE analysis was conducted on the apoplast extracts of NA and CA winter rye plants revealed the limitations of standard 1D SDS-PAGE. The 2-dimensional gel analysis revealed that there is a minimum of 25 proteins within the apoplast of CA winter rye, including at least 5 ??-1,3-endoglucanases. Genome walking was used to isolate cold-responsive glucanase genes. The five genes isolated were designated scGlu6, scGlu9, scGlu10, scGlu11 and scGlu12. The cis-element pattern within the promoter of each gene was evaluated using online databases of documented plant cis elements. As expected, all of the promoters contained elements associated with cold, biotic and abiotic stresses, light regulation, and development. The expression patterns predicted by the cis elements in each promoter were compared to the mRNA abundance produced by each gene as detected by semi-quantitative reverse transcriptase PCR. In most cases, the abundance of transcripts arising from each gene loosely corresponded to the expression pattern predicted by the cis elements the corresponding promoter. Transcripts of scGlu9, 10 and 11 were present in cold-treated tissues and are candidates for ??-1,3-endoglucanases with antifreeze activity. The results presented in this thesis provide additional insight into the apoplast proteome of CA winter rye plants as well as the complexity of the signals controlling the proteins that reside there. Although there are still a number of unresolved questions, this research opens new directions for future studies in the cold acclimation process in winter rye and specifically for the contribution of ?? -1,3-endoglucanses.

The main part of the thesis is evaluation of yield and qualitative parameters of hybrid winter rye. A small plot experiment was made at the Faculty of Agriculture of the University of South Bohemia in České Budějovice (380 m). The trial took place in two years: 2016/2017 and 2017/2018. The effect of sowing (early and late), seed quantity (standard - 2 MKS / ha and reduced - 1 MKS / ha), variety and year were evaluated in three varieties of hybrid rye (Cossani, Performer, Santini). To achieve the most accurate results, all 12 variants were sown in 4 replicates. From the qualitative parameters were determined: Falling number, volume weight, content of N substances. Concerning the yield, use of a standard seed rate (2 MKS / ha) was more stable, with an average yield increase of 0.64 t / ha. The most profitable was the Performer variety, which stood out with a consistently high falling number.

Cover crops have the potential to reduce environmental impacts of corn production. The objective of this study was to quantify differences in nitrogen (N) and phosphorus (P) loading between corn plots with or without a winter rye cover crop (Secale cerale). Four field plots (30 x 46 m) in Chazy, NY with edge-of-field monitoring were used for the study. Two plots were randomly assigned a rye cover crop treatment and planted with a grain drill at a rate of 112 kg ha-1 after corn silage harvest in 2015 and 2016. Continuous water flows were monitored from surface runoff and tile drain hydrologic pathways dur-ing runoff events. Soluble reactive P (SRP), total P (TP), nitrate-N, total N (TN), and to-tal suspended solids (TSS) concentrations were measured and multiplied by runoff vol-umes to estimate nutrient export. Surface runoff from rye plots had lower nutrient loss compared to control plots. Cumulative nitrate-N exports were similar between treatments (15.7 vs. 14.8 kg nitrate-N ha-1 for rye and control, respectively). Cumulative TN exports were numerically higher for control plots compared to rye plots, (18.8 vs. 21.4 kg TN ha-1). Cumulative TP and SRP exports (surface + tile) for rye were 2.2 and 3-fold greater than control plots, (0.51 vs. 1.19 kg TP ha-1 and 0.33 vs. 0.96 kg SRP ha-1). Total P and SRP loads in surface runoff were 3.0-fold greater for control plots compared to rye plots (0.36 vs. 1.12 kg TP ha-1 and 0.32 vs. 0.94 kg SRP ha-1). TSS load in surface runoff was numerically higher for control plots compared to rye (5.7 vs. 20.6 kg ha-1). Cumulative surface runoff was 1.8-fold greater in control plots compared to rye plots (112.6 mm vs. 207.7 mm), while cumulative tile runoff was numerically higher in rye plots compared to control (83.2 mm vs. 66.1mm). Snowmelt events contributed the majority of phosphorus losses (96% of SRP and 92% of TP), emphasizing the need to implement management techniques that reduce P transport risk during the non-growing season. Winter rye re-duced snowmelt TP export by 3-fold compared to the control plots (0.33 kg TP ha-1 and 1.03 kg TP ha-1). The winter rye cover crop planted after corn silage harvest effectively reduced erosion and P transport in surface water runoff compared to corn silage left fal-low after harvest. In addition to significantly reducing P exports, farms have the option of harvesting rye as a forage crop and double cropping with corn. In this way, more total forage is possible for the farm in addition to offering environmental conservation and wa-ter quality benefits.

A gel is a cross-linked polymer network that spans an entire liquid medium; its properties depend strongly on the interaction of the polymer and the liquid medium. There are various ways to induce gelation in different systems such as altering temperature or pH. In this study, phenol extracted protein fractions from non-acclimated (NA) and cold-acclimated (CA) winter rye (Secale cereale L. cv Musketeer) leaf tissue were subjected to freeze-thaw treatment. Gelation was induced in the NA and CA extracts after repeated freeze-thaw treatments, accompanied by a change in sample rheological properties. Further experimentation revealed that gel formation only occurred at high pH (pH 12.0) and that a minimum of 3 to 4 freeze-thaw cycles were required. The viscosity of the protein gel increased 5.7- to 9.5-fold in the NA and CA extracts respectively upon freeze-thaw. Experiments optimizing the extraction conditions and protein concentration were also performed. The gel was stable and only a specific combination of chaotropic agent, anionic surfactant and reducing agent such as urea, sodium docecyl sulfate (SDS) and â-mercaptoethanol (â-ME) with heating could disrupt the gel network. The gel was composed of several proteins in the extracts as determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Based on SDS-PAGE analysis, ribulose-1,5-bisphosphate carboxylase oxygenase (Rubisco) was identified as the major protein component in the gel. Various experiments were performed to assess the role of Rubisco in gel formation; however, the results were inconclusive. It is suggested that these extracts may contain antifreeze proteins (AFPs) that have been demonstrated to form amyloid gels upon freeze-thaw. Further studies examining the composition and mechanism of gel formation may result in a future role for this material in the food industry.

A gel is a cross-linked polymer network that spans an entire liquid medium; its properties depend strongly on the interaction of the polymer and the liquid medium. There are various ways to induce gelation in different systems such as altering temperature or pH. In this study, phenol extracted protein fractions from non-acclimated (NA) and cold-acclimated (CA) winter rye (Secale cereale L. cv Musketeer) leaf tissue were subjected to freeze-thaw treatment. Gelation was induced in the NA and CA extracts after repeated freeze-thaw treatments, accompanied by a change in sample rheological properties. Further experimentation revealed that gel formation only occurred at high pH (pH 12.0) and that a minimum of 3 to 4 freeze-thaw cycles were required. The viscosity of the protein gel increased 5.7- to 9.5-fold in the NA and CA extracts respectively upon freeze-thaw. Experiments optimizing the extraction conditions and protein concentration were also performed. The gel was stable and only a specific combination of chaotropic agent, anionic surfactant and reducing agent such as urea, sodium docecyl sulfate (SDS) and â-mercaptoethanol (â-ME) with heating could disrupt the gel network. The gel was composed of several proteins in the extracts as determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Based on SDS-PAGE analysis, ribulose-1,5-bisphosphate carboxylase oxygenase (Rubisco) was identified as the major protein component in the gel. Various experiments were performed to assess the role of Rubisco in gel formation; however, the results were inconclusive. It is suggested that these extracts may contain antifreeze proteins (AFPs) that have been demonstrated to form amyloid gels upon freeze-thaw. Further studies examining the composition and mechanism of gel formation may result in a future role for this material in the food industry.

Cover crops are often planted during the fallow periods of cash crop harvests to cover the soil and reduce erosion but also to provide other ecosystem benefits including capturing residual nutrients and thus, reducing environmental losses of nitrogen (N) and phosphorus (P) in agroecosystems. Among these cover crops, winter rye (Secale cereale) is popular due to its winter hardiness and relatively cheap seed costs. However, growers in the Midwest, USA are reluctant to use winter rye prior to corn (Zea mays L.) due to the potential yield penalty in corn. This thesis introduces two strategies that could minimize winter rye’s effect on corn while providing nutrient loss reduction benefits are precision planting and reducing the seeding rate of winter rye ahead of corn. One study evaluates whether precision planting (planting winter rye strategically to avoid intersecting zones with corn) of winter rye at low seeding rate (37.5 kg ha-1) could produce similar cover crop biomass and quality to normal planted winter rye (50 kg ha-1) and if precision planting can improve performance and N requirement of corn (Chapter 1). The study was conducted in central Indiana during 2020-2021 (CIN21), and southern Illinois during 2021-2022 (SIL22), and 2022-2023 (SIL23) growing seasons. The experiment was arranged in a randomized block design with split plot arrangement. Main plots were three cover crops (a no-cover crop control (NoCC), conventional planted winter rye (CR), and precision planted winter rye (PR). Subplots were six rates of N fertilizer that ranged from 0-280 kg ha-1 for the CIN21 and 0-359 kg ha-1 for SIL22 and SIL23. Our results indicated that shifting from normal planting to precision planting resulted in similar cover crop biomass production with limited effect on winter rye quality [N concentration, Carbon (C):N ratio] and N and C accumulation. In CIN21, the no-cover crop control had higher yield and lower N requirements which was consistent with those of SIL22. The economic optimum rate of N (EORN) was below the typical recommended range for central Indiana and was above the recommended range for southern Illinois. Precision planting resulted in a slight increase in corn yield and N requirement, but overall was more profitable than normal planting due to a reduction in the number of seeds required and higher corn to fertilizer prices. Therefore, we recommend that (i) decision support tools for N management in corn should be revised for addition of cover crops in the Midwest, and (ii) precision planting should be implemented instead of normal planting for greater economic benefit. Future research should evaluate ecosystem services of precision vs. normal planting of winter rye over time. The other study evaluates whether planting method of winter rye (precision vs. conventional) at medium and low seeding rates of winter rye influence cover crop biomass production, N and C concentrations and accumulations, and corn performance (Chapter 2). A trial was conducted in 7 site-yrs in Indiana and Illinois during 2020-2021, 2021-2022, and 2022-2023 growing seasons. The trial was arranged in a randomized complete block design with four replicates. Cover crops [conventional planting (CR) and precision planting (PR)] were factorially arranged with two seeding rates (18.75 vs. 37.5 kg ha-1) for PR and (25 vs. 50 kg ha-1) for CR. Two extra treatments were included as control which were no-cover crop with zero-N and a 224 kg N ha-1 addition to corn. Cover crop biomass, C, N, their uptake, and C:N ratio were evaluated along with corn plant population, and corn grain yield. Our results indicated that winter rye had similar aboveground biomass, N uptake, and C accumulation regardless of planting method and seeding rate suggesting a precision planting at low seeding rate is most economical for cover crop establishment. Corn plant population was only affected by winter rye in one site-yr (CIL23) in which precision planting did not help with minimizing the negative effect of winter rye on corn population. In this study, lack of N fertilization did not decrease corn population but significantly reduced corn grain yield in all site-yrs. Corn grain yield was similar among cover crop treatments (with exception of no cover crop no N) but in one of the site-yrs, precision planting at 18.75 kg ha-1 resulted in greater corn yield than the no-cover crop with 224 N ha-1. We concluded that growers that plant winter rye prior to corn could use precision planting at a seeding rate of 18.75 kg ha-1 to take up residual soil N with limited interference with corn production at a reduced cost compared to conventional winter rye management.

Winter rye produces ice-modifying antifreeze proteins upon cold treatment. Two of these antifreeze proteins are members of the large, highly conserved, β-1,3-endoglucanase family. This project was designed to identify glucanase genes that are expressed during cold acclimation, wounding, pathogen infection, drought or treatment with the phytohormones ethylene and MeJa. Additionally, a more detailed proteomic analysis was to be carried out to evaluate the glucanase content of the apoplast of cold-acclimated (CA) winter rye. Results of 2D SDS-PAGE analysis revealed that non-acclimated whole leaf protein extracts contain at least two β-1,3-endoglucanses while CA whole leaf protein extracts contain at least three β-1,3-endoglucanses. Subsequent 2D SDS-PAGE analysis was conducted on the apoplast extracts of NA and CA winter rye plants revealed the limitations of standard 1D SDS-PAGE. The 2-dimensional gel analysis revealed that there is a minimum of 25 proteins within the apoplast of CA winter rye, including at least 5 β-1,3-endoglucanases. Genome walking was used to isolate cold-responsive glucanase genes. The five genes isolated were designated scGlu6, scGlu9, scGlu10, scGlu11 and scGlu12. The cis-element pattern within the promoter of each gene was evaluated using online databases of documented plant cis elements. As expected, all of the promoters contained elements associated with cold, biotic and abiotic stresses, light regulation, and development. The expression patterns predicted by the cis elements in each promoter were compared to the mRNA abundance produced by each gene as detected by semi-quantitative reverse transcriptase PCR. In most cases, the abundance of transcripts arising from each gene loosely corresponded to the expression pattern predicted by the cis elements the corresponding promoter. Transcripts of scGlu9, 10 and 11 were present in cold-treated tissues and are candidates for β-1,3-endoglucanases with antifreeze activity. The results presented in this thesis provide additional insight into the apoplast proteome of CA winter rye plants as well as the complexity of the signals controlling the proteins that reside there. Although there are still a number of unresolved questions, this research opens new directions for future studies in the cold acclimation process in winter rye and specifically for the contribution of β -1,3-endoglucanses.

Tiriamojo darbo objektas – žieminiai javai išauginti ekologinės ir intensyvios žemės ūkio gamybos ūkiuose Raseinių raj. Girkalnio ir Nemakščių seniūnijose. Tyrimo tikslas - ištirti javų žiemojimo ypatumus ekologinės ir intensyvios žemės ūkio gamybos ūkiuose. Tyrimo metodika. Tyrimas buvo atliktas 2008 – 2009 m. Raseinių rajono Girkalnio ir Nemakščių seniūnijos ūkiuose. Tyrimų dirvožemis – išplautžemis. Buvo pasirinkti 2 intensyvios ir 2 ekologinės žemės ūkio gamybos ūkiai. Šiuose ūkiuose buvo auginami žieminiai javai (rugiai, kviečiai ir kvietrugiai). Augalų skaičiavimo vietų skaičius buvo nustatomas pagal lauko dydį. Javų lauke atsitiktinai išskiriamos vietos po 0,25 m2 tyrimo laukelius kiekvieno lauko pradžioje, viduryje ir gale. Kiekvienoje vietoje suskaičiuojami augalai vegetacijos pradžioje ir prieš derliaus nuėmimą. Gauti duomenys perskaičiuojami į 1 m2. Pasinaudojant rudens ir pavasario augalų tankumo duomenimis buvo apskaičiuotas žiemojimo procentas. Tarp augalų skaičiaus rudenį ir peržiemojimo procento apskaičiuoti koreliaciniai ryšiai. Taip pat apskaičiuoti koreliaciniai ryšiai tarp augalų tankumo ir oro temperatūros. Tyrimo rezultatai. Atlikus tyrimus minėtuose laukuose suskaičiuota, kad ekologinės žemės ūkio gamybos ūkiuose tankiausi buvo žieminiai rugiai. Jų augalų skaičius įvairavo nuo 344 iki 580 vnt. m2, o intensyvios – žieminiai kvietrugiai, kurių priskaičiuota nuo 380 iki 568 augalų. Pavasarį tiek žieminių rugių, tiek ir žieminių kvietrugių išliko... [toliau žr. visą tekstą] / The study objective – to explore the characteristics of organic winter cereals and intensive agricultural production on farms. Objective – winter crops have been grown in organic and intensive agricultural production on farms Raseiniu region. Method of the work. The study was carried out in 2008 - 2009 on Raseiniu Girkalnio district and parish Nemakščių farms. Soil surveys - Luvisol. It was choice two and two intensive organic agricultural production of farms. These holdings have grown winter cereals (rye, wheat and triticale). Plant counting the number of sites were determined by field size. Cereal field identifies the local random 0.25 m2 for each field of study in the fields early, middle and rear. Each site counted at the beginning of vegetation and plants before harvest. The data obtained are converted into 1 m2. Through the autumn and spring plant density was estimated wintering percent. Among the number of plants in autumn and peržiemojimo calculate percent correlation. Also calculate the correlation between plant density and air temperature. The results of the work. After research in these fields has been calculated that organic agricultural farms were densest winter rye. Their number of plants varied from 344 to 580 units. m2, and intense - winter triticale, which counted from 380 to 568 plants. In spring and winter rye and winter triticale, and remained the least. Among the studies found an average variation - from 10 to 20 percent.Cereal hibernation depends on... [to full text]

Langzeitdüngungsversuche (LTFE) sind für die Agrarforschung von entscheidender Bedeutung, da sie dokumentieren, überwachen, lernen und zeigen können, was in der Vergangenheit geschehen ist, und mit Hilfe von Vorhersagemodellen vorhersagen und simulieren können, was in Zukunft geschehen wird. Diese Modelle dienen dazu, zukünftiges Pflanzenwachstum unter verschiedenen Klima- und Bewirtschaftungsszenarien abzuschätzen und so Entscheidungsprozesse zu unterstützen. In diese Studie wurden die Reaktionen der Getreideerträge auf das Düngermanagement in Sandböden in einem LTFE (1971 bis 2016) in Nordostdeutschland analysiert. Die Ziele dieser Studie waren a) die Analyse der Ertragsreaktionen von Sommergerste, Winterroggen und Winterweizen auf das Düngemanagement, b) die Analyse der Sensitivität der Ertragsreaktionen auf den Zeitpunkt von Wetterereignissen und c) der Vergleich verschiedener Analysemodelle. Die Studie ergab, dass die Reaktion der Getreideerträge auf das Düngermanagement von komplexen Beziehungen zwischen Klimaabhängigkeit, Vorfrucht und Bodeneigenschaften beeinflusst wurde. Die Witterungsbedingungen bei der Aussaat und in den frühen Wachstumsstadien des Getreides beeinflusste den Kornertrag. Bei Wintergetreide waren die Intensität und Dauer der extremen Temperaturen im Sommer, insbesondere die Anzahl der Tage mit einer Höchsttemperatur von über 30°C im Juli, eine wichtige Variable für den Ertrag. Unter den untersuchten Modellen zeigte das LMM-Modell eine bessere Vorhersageleistung als das M5P-Modell, und beide hatten umfangreichere Regressoren als die ANOVA und die BMA. Das M5P-Modell bot eine intuitive Visualisierung wichtiger Variablen und ihrer kritischen Schwellenwerte und offenbarte andere Variablen, die vom LMM-Modell nicht erfasst wurden. Die Ergebnisse dieser Analyse tragen zu umfassenden Strategien für eine nachhaltige Pflanzenproduktion im Hinblick auf den zukünftigen Klimawandel bei. / Long-term fertilizer experiments (LTFEs) are essential for agricultural research as they provide necessary information and data to build predictive models. These models can be used to estimate future plant growth under different climate and management scenarios to support decision-making processes. The current study analyzed cereal grain yield responses to fertilizer management in sandy soil in a LTFE (1971 to 2016) in Northeast Germany. The objectives of this study were to a) analyze yield responses of spring barley), winter rye, and winter wheat to fertilizer management, b) analyze the sensitivity of yield responses to timing of weather events, and c) compare different analysis models. The study revealed that cereal yield response to fertilizer management was influenced by complex relationships among climatic dependence, preceding crop, and soil characteristics. Weather conditions at seeding and early growth stages of cereal were found to be sensitive to grain yield. For winter cereals, the intensity and duration of extreme temperatures in the summertime (harvest year), especially the number of days recorded with a maximum temperature above 30°C in July was an important variable for the yield. Among the investigated models, LMM-model had a better predictive performance compared to M5P-model and both had richer regressors than the ANOVA and BMA-model. The M5P-model presented an intuitive visualization of important variables and their critical thresholds, and revealed other variables that were not captured by the LMM-model. The findings of this analysis contribute to comprehensive strategies for sustainable crop production with regard to future climate change.