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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
121

A study of zebrafish hematopoiesis based on chemical screening and gene knock-down by morpholino with particular reference to ADP-ribosylation factor like 4 (ARL4)

Man, Hon-wai., 文漢威. January 2011 (has links)
Zebrafish has emerged as an important vertebrate model for studying hematopoiesis and its genetic and chemical modifiers. The zebrafish embryos are unique in their optical transparency, ease of maintenance and high fecundity. They are also amendable to genetic and pharmacological perturbation at high throughput. As a result, the embryos are suitable for various experimental techniques and have a high efficiency in large-scale drug screening. Recently, zebrafish has also emerged as a model for the study of human disease. In this model organism, primitive hematopoiesis is transitory and it occurs in the intermediate cells mass and comprises primarily erythroid cells. Definitive hematopoiesis arises from the ventral wall of dorsal aorta and moves to the caudal hematopoietic tissues, thence the kidney, where life-long and multi-lineage differentiation occurs. The chemical screening platform comprises O-dianisidine staining for hemoglobin containing cells (erythroid) during primitive hematopoiesis. Positive hits were validated based on flow cytometry of dissociated transgenic Tg(gata1:GFP) embryos and whole-mount in-situ hybridization (WISH) for hematopoietic genes. Gene knock-down was conducted by morpholinos (MO) injected into zebrafish embryos at 1-4 cell stage and the effects on hematopoietic development evaluated by WISH and quantitative real-time PCR. Chemical screening of 74 compounds has been performed. These compounds were obtained from a chemical library comprising 879 compounds from NIH (National Institutes of Health) and pre-screened by their effects on cancer cell lines. Four compounds (Tin(IV), chlorotriphenyl [1-(4-ethoxyphenyl)- 3-cyanoureato]-hydrogen,triethylamine, Nogamycin, N,N-Dibenzyldaunorubicin hydrochloride and Allyl 2,3,4-tri-O-benzyl-6-O-(tert-butyldimethylsilyl)-α- D-gluco-Pyranoside) which significantly reduced O-dianisidine staining were identified of which one (Allyl 2,3,4-tri-O-benzyl-6-O-(tert-butyldimethylsilyl) -α-D-glucopyranoside was shown to reduce GFP+ population in Tg(gata1:GFP) population Another chemical (2-Propanol,1,1'-[(1-methylethylidene)bis(4, 1-phenyleneoxy)]bis[3-[(1,1,3,3-tetramethylbutyl)amino]-,dihydrochloride]) was shown to reduce c-myb (marker of definitive hematopoiesis) expression in the ventral wall of dorsal aorta. I also attempted gene knock in zebrafish embryos based on anti-sense morpholino microinjection. A gene encoding for arl4ab was examined, as it was shown to be expressed in hematopoietic tissue in zebrafish embryos but its function is entirely unknown. Knock-down of arl4ab significantly reduced c-myb and runx1 expression in the ventral wall of dorsal aorta and it can be reversed by co-injecting arl4ab mRNA. scl and gata1 expression as well as GFP expression in transgenic Tg(flk1:GFP) embryos that represented vascular development was unaffected. In summary, a zebrafish platform for the study of chemical and genetic modifiers was established. The results have provided important leads for further study into the mechanisms whereby these modifiers regulate hematopoiesis in the zebrafish embryos. / published_or_final_version / Medicine / Master / Master of Medical Sciences
122

Molecular characterizations of chicken and zebrafish prostanoid receptors and their implications on evolution of vertebrate prostanoidreceptor family

Kwok, Ho-yan, Amy., 郭可茵. January 2011 (has links)
Prostanoid receptors (PG-Rs: prostaglandin D, E, F, prostacyclin and thromboxane receptors (DP, EP1-4, FP, IP and TP)) are known to mediate a diverse range of biological responses, such as cardiovascular homeostasis, nociception and reproduction, via binding to their respective ligands belonging to the five classes of prostanoids (PGs: class D, E, F, I and thromboxane). The majority of these findings were reported in mammals, and despite suggestive evidence provided by previous pharmacological and physiological studies in non-mammalian vertebrates, investigations on the mechanisms behind actions of PGs were impeded by the limited information on their receptors. In the present study, the full-length cDNAs of chicken (c-) and zebrafish (z-) prostanoid receptors – cEP3, cFPs, zEP1s and zFP – were identified from respective adult ovaries and their tissue distribution examined by RT-PCR. A novel middle-truncated splice variant, cFPb, which lacks 107 amino acids between transmembrane domains 4 and 6 but otherwise identical to cFPa was first identified. Three isoforms of zEP1 – zEP1a, zEP1b, zEP1c – were found, which might have subfunctionalized in their ligand binding and G protein coupling specificity, in addition to differential tissue distribution. Using various luciferase reporter systems (pGL3-CRE, pGL-NFAT-RE, pGL4-SRE), all the cloned receptors, except cFPb, were shown to potentially couple to intracellular cAMP, Ca2+, and/or MAPK signaling pathways. Owing to the proposed roles of PGs and its potential regulation by and/or on EGFR ligands and gonadotropins in mammals and chicken, genes involved in regulation of PG functions at various levels, including biosynthesis (COX1, COX2, mPGES1, mPGES2 and cPGES), availability (PGT) and signaling (cEPs and cFPs), were also characterized in granulosa cells during hen follicular development. Lastly, using our experimental data and systematic sequence retrieval from available databases, the PG receptor cascades from representative vertebrate species were pooled and analysed using phylogenetic analyses and synteny studies. Three putative clusters (IP-like, EP4-like and EP1-like cluster) were found in lamprey genome; meanwhile, only one PG-R-like cluster was identified from the Cephalochordate lancelet (amphioxus) genome. This concurs with the 1-2-4 rule proposed in first round/second round (1R/2R) whole genome duplication in which the missing lamprey cluster was presumably lost secondarily. With support from conserved orthologs-localization, the four PG-R paralogs (proto-EP4, proto-IP/EP2/DP, proto-TP/FP/EP1 & putative proto-EP3 genes) in the ancestral vertebrates might have further diversified via either localized- (e.g. EP2 and DP) or chromosomal segmental duplication (e.g. EP1, FP and TP) which resulted in the present array of vertebrate PGRs. Additional paralogs (e.g. EP1 and EP4) were identified from fishes, by which molecular dating coincide with and hint of their origins whence the ancient fishspecific whole genome duplication (3R) occurred ~350 million years ago. The present study offers the first glimpse and a better understanding of the roles of the PG-Rs and presents a higher resolution to the evolutionary history of each PG-R family member, consolidating that particular care has to be taken when studying non-mammalian PG-R functions in which some members are absent or present in multiples and propel the investigation of adaptational changes in the coding sequence during evolution of vertebrate PG-Rs. / published_or_final_version / Biological Sciences / Doctoral / Doctor of Philosophy
123

Using zebrafish as a model organism for the study of embryonic hematopoiesis based on chemical screening and genetic manipulation

Ng, Koon-kiu., 吳官橋. January 2013 (has links)
Zebrafish has emerged as an important model for the study of embryonic hematopoiesis. It is a well-characterized model with numerous advantages. Large amount of embryos can be produced by a single pair of zebrafish and the optically transparent embryos allow direct visualization and manipulation of embryonic processes. Large-scale chemical screening using zebrafish embryos can be developed for robust screening of chemical libraries. The zebrafish hematopoiesis resembles that of mammals and occurs in two successive waves, primitive and definitive hematopoiesis. High-throughput read-outs are available to study the effects of different chemicals and genetic modifications on hematopoiesis. In first part of this study, an initial screening using O-dianisidine staining and whole-mount in-situ hybridization as read-out for chemicals that might perturb the regulation of hematopoiesis was conducted. Positive hit was further evaluated by flow cytometry of dissociated transgenic Tg(gata1:GFP) zebrafish embryos. A total of 50 compounds were screened from the "Mechanistic set" chemical libraries obtained from Developmental Therapeutics Program of the National Cancer Institute. One compound, "NSC 643834" was shown to reduce O-dianisidine staining at different concentrations tested. The second part of this study was performed to investigate the role of inca2 in zebrafish hematopoiesis. inca2 was found to be upregulated in chordin morphant zebrafish in which primitive hematopoiesis was expanded. The spatial expression of inca2 was examined by whole mount in-situ hybridization of embryos at different developmental stages. Furthermore the function of inca2 was investigated by gene knockdown using inca2 anti-sense morpholino. Primitive hematopoiesis was perturbed, suggesting that inca2 might play an important role in the regulation of this process. In conclusion, the present study demonstrated the distinct advantages and feasibility of using zebrafish as a platform of high throughput chemical screening and genetic manipulation. The result provided important ground to investigate the regulatory mechanisms of embryonic hematopoiesis. / published_or_final_version / Medicine / Master / Master of Research in Medicine
124

Studies of adaptive immune responses in zebrafish (Danio rerio), with a focus on the role of CD4+ cells

Yoon, Sohye January 2014 (has links)
CD4+ lymphocytes (T helper cells) play a crucial role in orchestrating adaptive immune responses. This project aims to characterise the CD4+ cells in zebrafish (Danio rerio) for a better understanding of adaptive immunity in teleost fish. I cloned three CD4 homologues, termed zfCD4 and zfCD4 related (zfCD4rel1 and zfCD4rel2), with both reported previously in other bony fish species. The zfCD4 and zfCD4rels transcripts were detected in immune organs in zebrafish and were most highly expressed in the lymphocyte population. A moderate induction of the zfCD4 and zfCD4rels (and other immune related genes) was seen in kidney, spleen and intestine after poly (I:C) injection. Two antibodies against CD4 and IFN-γ, respectively, have been validated using various immunostaining approaches for further functional studies. The CD4 positive cells ranged from 20-30% of lymphocytes in zebrafish, similar to what is seen in other vertebrates. The expression level of IFN-γ and other Th cell related genes were analysed in immunised fish following re-stimulation with antigen, revealing that in zfCD4+ lymphocytes an increased expression of cytokines and master transcript factors was seen when the same antigen was used for boosting. This is the first demonstration of Th-type responses effected by CD4+ lymphocytes in a poikilotherm. Lastly, I studied an aspect of Treg function in zebrafish, focused on a master transcription factor of Tregs, the FoxP3 gene. Knocking down FoxP3 genes in zebrafish resulted in modulated gene expression of cytokines and transcription factors associated with Th and Treg cells, providing some evidence that the immune tolerance function of Treg cells may exist in teleost fish, with some sub-functionalisation of the two FoxP3 paralogues apparent. This thesis extends our knowledge into teleost adaptive immune responses mediated by CD4+ Th cells and putative FoxP3+ Treg cells and may aid future studies using zebrafish as a model of vertebrate immune function.
125

Imaging fusion and retrieval of synaptic vesicles in retinal bipolar synapses of zebrafish

Pelassa, Ilaria January 2011 (has links)
No description available.
126

Effect of the habitat fragmentation on the Grévy’s zebra population genetic structure

Khalatbari, Leili January 2013 (has links)
The exponential growth of the human population is limiting the wildlife habitat all around the word. In recent years habitat loss and fragmentation is one of the main reasons that threats the wild life species. The Grévy’s zebra (Equus grevyi) is the most endangered member of Zebras. Their historical range was previously from north Ethiopia to southwest Somalia and to northern Kenya. Currently they are distributed only in fragmented habitats in central and eastern part of Ethiopia and in the north of Kenya. They are listed as endangered in the IUCN red list, as their population has declined 68% in 27 years. There are very few studies on genetic structure of this species, and investigating the genetic connection between different populations is needed. Molecular markers are one of the best tools to understand the level of fragmentation, population bottlenecks or potential inbreeding. In this study, the population structure of Ethiopian zebra population from Alledeghi Wildlife Reserve (WR) and Sarite area was studied using non-invasively obtained fecal samples collected during 2001-2011. This study analyzes genetic variation at 10 microsatellite loci and a 350-bp fragment of the mitochondrial DNA control region. The results showed that the genetic diversity is very low between the populations (π=0.00116 for Alledeghi WR and π=0 for Sarite population). The population of Alledeghi WR is probably isolated from the population of Sarite, as they don’t share any haplotypes. As the population of Alledeghi WR is separated from the ones from Sarite and Kenya, applying more conservational programs in this area is needed to protect the genetic diversity of the Grévy’s zebras in this area.
127

Metabolic power budgeting in fishes : laboratory studies in zebra fish, Brachydanio rerio and heart-rate telemetry in pike, Esox lucius

Lucas, Martyn Charles January 1989 (has links)
Metabolic power budgeting, the regulation of metabolism with respect ot metabolic scope, was studied in the laboratory in zebra fish using respirometry, and in the field on pike using heart-rate telemetry. Increased food consumption by zebra fish resulted in higher growth, mortality and metabolism. The magnitudes of the components of metabolism: maximum metabolism, standard metabolism, routine metabolism and feeding metabolism were measured. Power budgets for zebra fish fed high and low rations were constructed. Fish fed high rations worked harder than fish fed lower rations, but were apparently not working near the upper limit of the metabolic scope. Possible mechanisms for growth-related mortality are considered. Biological information on the populations of pike in Lochs Kinord and Davan (Grampian Highlands) were gathered. The population of L. Kinord was dominated by young, small fish; apparently due to exploitation. L. Davan is unexploited and had a pike population consisting of a much wider range of ages and sizes. Methods for assessing regurgitation by pike were developed. Effects of long and short-term temperature fluctuations, and feeding on heart rate of captive pike were studied. Resting heart rate increased exponentially with increasing temperature; heart rate appeared to accommodate all changes in resting metabolism. Post-prandial heart-rate records could be used to accurately estimate meal size. Gastric evacuation rates corresponded to digestion times estimated from heart-rate records. Heart-rate telemetry was used to study metabolic power budgeting, feeding and activity of wild pike from Lochs Kinord and Davan in June 1988. Pike worked mainly at low power levels relative to metabolic scope. Tachycardias associated with localized movement were frequent, and such movement was accurately recorded by heart-rate telemetry but frequently undetected by conventional means. Feeding events were identified and the metabolic costs of survival estimated. Some unusually energetically-expensive localized movements were recorded; the possible reasons for this are discussed. Intraperitoneal implantation techniques were developed for transmitter attachment on pike. Experments using dummy transmitters on pike and rainbow trout showed no effect on growth, survival or reproduction, but tissue reactions differed. Male and female pike, location-tracked with implanted transmitters before, during and after spawning time exhibited increased overall activity during the apparent spawning period, as well as changes in diet activity. Males were significantly more active than females in three out of seven weeks. Spawning appears to be a period of high energy expenditure for pike.
128

Patterns in the distribution and abundance of zebra mussels (Dreissena polymorpha) in the St. Lawrence River in relation to substrate and other physico-chemical factors

Mellina, Eric January 1993 (has links)
Using SCUBA and an in situ method of quantifying substrate characteristics, I describe patterns of zebra mussel (Dreissena polymorpha) distribution along the St. Lawrence and Hudson Rivers and in Oneida Lake, New York, and develop empirical models for their abundance. Calcium-poor waters originating from rivers draining the Canadian Shield resulted in a lack of zebra mussel along the north shore of the St. Lawrence River east of Montreal until Portneuf despite an abundance of suitable substrate. Calcium concentrations of 15 mg/L or less were found to limit the distribution of zebra mussel. The entire south shore from Cornwall, Ontario to Ile d'Orleans, Quebec was colonized by zebra mussel wherever suitable substrate was found. In the Hudson River, along the south shore of the St. Lawrence River and in Oneida Lake variability in density was primarily related to substrate type which explained between 38% and 91% of the variance. Other factors such as Secchi depth, calcium concentration of the water, the presence of crayfish, native unionid abundance and the maximum width of the river at the site increased the amount of explained variance across the different systems. The influence of substrate type on zebra mussel density and the predictions of the model were also tested using data from the literature, where substrate type explained 75% of the variability in density. The scatter of the literature data above the predictions of the empirical model suggests that North American zebra mussel populations may continue to grow before reaching equilibrium levels. While water chemistry parameters may be useful predictors of the presence or absence of zebra mussel in a given water body, physical factors play a far greater role in determining local abundance.
129

Einfluss von Haltunsbedingungen im Zoo auf endokrine Stressparameter bei Zebras

Trapp, Juliane 20 May 2011 (has links) (PDF)
Mit dieser Arbeit sollte erforscht werden, welchen Einfluss Umsetzung und Vergesellschaftung von Grevy- bzw. Böhm-Zebras in Zoologischen Gärten auf die Produktion von Stressund Sexualhormonen haben. Dafür wurden vor, während und nach dem Umsetzungs- und Vergesellschaftungsprozess Kotproben der Zebras gesammelt,bei -20°C gelagert, mit einem Methanol-Wasser-Gemisch extrahiert und anschließend deren Gehalt an Cortisol-, Progesteron-, Östradiol- und Testosteronmetaboliten analysiert. Dabei konnte gezeigt werden, dass sowohl bei den adulten Grevy-Zebrastuten als auch bei deren Jungtieren (Zoo Leipzig) im Untersuchungszeitraum 2004 das ranghöchste und rangniedrigste Tier, 2008 nur das dominante Tier die höchsten Cortisolspiegel der Gruppe aufwiesen. Das in der Rangordnung den mittleren Platz einnehmende Zebra zeigte signifikant niedrigere Cortisolspiegel. Von den drei untersuchten Böhm-Zebras (Zoo Halle) besaß der Hengst signifikant niedrigere Cortisolwerte als die beiden Stuten, deren Cortisolspiegel sich vor der Umsetzung nicht signifikant voneinander unterschieden. Die Umsetzung der Grevy- Zebras in die Kiwara-Savanne und die Vergesellschaftungen mit Säbelantilopen, Straußen und Giraffen und auch die Umsetzung der Böhm-Zebras in ihr neues Gehege erhöhten jedoch nur bei den subdominanten Tieren den Basiswert des Cortisols signifikant. Zusätzliche Faktoren, wie z. B. sozialer Status (dominant vs. subdominant), soziale Ordnung und weitere exogene Einflüsse scheinen somit eine entscheidende Rolle bei der Stress-Verarbeitung von in Zoologischen Gärten gehaltenen Zebras einzunehmen. Die Adaptation der Zebras an ihre neue Umgebung nahm in den beiden Zoos unterschiedliche Zeitspannen in Anspruch. Bei den Grevy-Zebras war es aus tiergärtnerischen Gründen nicht möglich, diese AdaptationsZusammenfassung zeit zu definieren, da nur bis zwei Monate nach der Umsetzung eine individuelle Kotprobensammlung erfolgte und zu dieser noch bei einzelnen Tieren erhöhte Cortisolwerte messbar waren. 2008 konnten jedoch bei den adulten Stuten signifikant niedrigere Cortisolspiegel gemessen werden als 2004 im alten Gehege. Bei den drei untersuchten Steppenzebras fielen ca. vier Wochen nach der Umsetzung die Cortisolkonzentrationen deutlich ab und waren bei den beiden Stuten signifikant niedriger als im alten Gehege und als in der Zeit direkt nach der Umsetzung. Dies lässt die Schlussfolgerung zu, dass die neuen Gehege günstigere Umweltbedingungen bieten als die alten Zebra-Anlagen. Bei der Auswertung der Sexualhormone wurde deutlich, dass alle drei Grevy-Zebrastuten im nicht tragenden Zustand sehr ähnliche Sexualhormonspiegel zeigten. Nach der Umsetzung war weder für Progesteron noch für Östradiol ein charakteristischer Zyklusverlauf zu erkennen. Es ist also anzunehmen, dass hier der durch die Umsetzung und Vergesellschaftung hervorgerufene Stress und damit das Ansteigen der Cortisolproduktion einen negativen Einfluss auf die Reproduktionsfähigkeit der untersuchten Zebras hatte. Die Böhm-Zebras waren während der Umsetzung tragend. Die Narkose und die neue Umgebung hatten keinen messbaren Einfluss auf die Sexualhormone und am Ende einer physiologischen Tragezeit wurden zwei gesunde Jungtiere geboren. Bei den beiden Böhm-Zebrastuten konnten des Weiteren die Östradiol- und Progesteronexkretionsprofile über drei Jahre hinweg aufgenommen und somit die Trächtigkeitsdauer und der Hormonverlauf während der Trächtigkeit beschrieben werden. Das Hormonprofil des Östradiol gleicht weitestgehend dem bei Grevy- Zebras geschilderten Verlauf mit der maximalen Ausscheidung während des zweiten Trimesters. Die Konzentration der Progesteronmetabolite weicht jedoch deutlich von dem bei Grevy- Zebras beschriebenen Profil ab. Hier konnte ein stetiges Ansteigen der Werte ermittelt werden, wobei frühestens ab der 8. Woche a.p. Werte über 200ng/g erreicht wurden und die höchsten Konzentrationen ca. 12 bis einen Tag a.p. bzw. einen Tag post partum gemessen wurden. Die durchschnittliche Trächtigkeitsdauer betrug 343±15 Tage. Mit dieser Arbeit konnte gezeigt werden, dass mittels Hormonbestimmungen im Kot von Zebras Veränderungen im Hormonhaushalt sowohl von Stress- als auch von Sexualhormonen sehr gut quantifiziert werden können. Dadurch besteht die Möglichkeit, den Einfluss tiergärtnerisch wichtiger Verfahren, wie das Umsetzen und Vergesellschaften von Zebras oder die Kontrolle von Trächtigkeiten wissenschaftlich auszuwerten und Aussagen über die Qualität der Haltungsbedingungen zu treffen. Des Weiteren können über die individuellen Cortisolmetabolitkonzentrationen Rückschlüsse auf die soziale Stellung innerhalb der Gruppe gezogen und die Auswirkungen dieser auf den Umgang mit „Stress“ charakterisiert werden.
130

Characterization of genetically labeled dopamine neurons and circadian studies of the zebrafish retina

Meng, Shi, January 2008 (has links)
Thesis (M.S. in Biological Sciences)--Vanderbilt University, May 2008. / Title from title screen. Includes bibliographical references.

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