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Stanovení biologické aktivity rekombinantního proteinu adiponektinu pomocí buněčné kultury / Determination of biological activity of adiponectin, a recombinant protein using cell culturePernicová, Iva January 2016 (has links)
This thesis deals with the determination of biological activity of adiponectin, a recombinant protein using cell culture. First it was important to acquire the working skills for the cell culture of cell line 3T3-L1. An optimal concentration of inactivated fetal bovine serum in cell culture media was determined. A stimulation of the cell proliferation by HB-EGF, PDGF-BB and bFGF growth factors was observed at various concentration levels. Afterwards the biological activity of adiponectin was determined as an inhibition of growth stimulation with 5 ng/ml PDGF-BB. This biological activity assay for adiponectin was also conducted with lyophilized adiponectin and a growth factor bFGF (0.1 ng/ml). The lyophilization did not affect the biological activity of adiponectin.
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Nucleus-localized adiponectin is survival gatekeeper through miR-214-mediated AIFM2 regulation / 核局在アディポネクチンはmiR-214とAIFM2の経路を介して細胞の生存を制御するCho, Junkwon 25 March 2019 (has links)
京都大学 / 0048 / 新制・課程博士 / 博士(医科学) / 甲第21695号 / 医科博第99号 / 新制||医科||7(附属図書館) / 京都大学大学院医学研究科医科学専攻 / (主査)教授 生田 宏一, 教授 Shohab YOUSSEFIAN, 教授 齊藤 博英 / 学位規則第4条第1項該当 / Doctor of Medical Science / Kyoto University / DFAM
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Associations Between Physical Activity Level and Adiponectin/Leptin Ratios in Older AdultsMcMath, Arden Lee 03 May 2019 (has links)
No description available.
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The Regulation of Adiponectin by Ethanol Feeding and Taurine SupplementationChen, Xiaocong January 2009 (has links)
No description available.
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Omega-3 Fatty Acids and Heart FailureO'Shea, Karen Michelle 23 January 2010 (has links)
No description available.
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Determination of the Effect of Raspberry Ketone on Markers of Obesity in High-fat Fed C57BL/6 Male MiceDiamond, Stephanie A. 28 May 2015 (has links)
No description available.
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Immunomodulation During Systemic InflammationKaplan, Jennifer Melissa 06 August 2007 (has links)
No description available.
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The Effects of Diet Composition and Weight Loss on Serum AdiponectinSummer, Suzanne Spang 08 October 2007 (has links)
No description available.
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Increased Adiponectin is Associated with Left Ventricular Mass Index in Pediatric Chronic Kidney DiseaseLo, Megan M. 20 September 2011 (has links)
No description available.
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Investigation of adiponectin and its receptors in mouse-models of altered growth hormone action: Attempts to understand the link between adipose tissue and longevityLubbers, Ellen MR 20 June 2012 (has links)
No description available.
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