<p> A gap exists in the literature on the actual experiences of older workers with information and communication technology adoption in technology-infused workplaces. To inform organizations on how to more effectively support this employee group's adoption of technology, the purpose of this qualitative exploratory multiple case study was designed to gain a deeper understanding of the daily experiences of older workers when adopting and adapting to information and communication technologies in a technology-infused workplace. This study is framed by, first, the <i>selection-optimization-compensation framework for successful aging</i> and, second, the <i>age-inclusive training design framework.</i> Semistructured interviews with 8 participants, observational field notes, and archival data provided data regarding specific technology experiences among older workers in the workplace. Identifiable themes emerged through thematic analysis of the textual data and cross-case synthesis analysis. A total of 8 categories that enclose a total of 18 themes were identified. The categories are (a) selection of resources, (b) optimization of resources, (c) compensation of resources, (d) performance limitations, (e) assessing training needs, (f) establishing/ sustaining performance, (g) age-inclusive training needs, and (h) older workers as organizational assets. Findings enhanced social change efforts by providing insight on the daily experiences that the older worker faced which may contribute to limited productivity in the workplace. Organizational leaders and human resource managers may use results of this study to implement provisions that improve help organizational profitability, team cohesiveness, and workplace satisfaction.</p><p>

Both an intuitive observation and maybe the most mysterious process of biology, aging describes the progressive deterioration of cellular functions with time. Asymmetric cell divisions stand at the center of ability to reset age in offspring and for stem cells to self-renew. This requires the asymmetric segregation of age-determinants, many of which have been identified in the budding yeast Saccharomyces cerevisiae. We here use budding yeast to explore fundamental aspects underlying the asymmetric inheritance of mitochondria and the concurrent rejuvenation of daughter cells. We show that in addition to the preferential inheritance of high-functioning mitochondria to daughter cells, a distinct population of high-quality organelles must also be retained within the mother cell. We find that both physical retention and qualitative maintenance of a distinct mitochondrial population at the mother cell tip depends on Mitochondrial F-box protein (Mfb1p) and that MFB1-deletion leads to premature aging. Our findings outline a critical balance between the need for daughter cell rejuvenation and the requirement to conserve replicative potential within the mother cell. The particular mechanism by which Mfb1p functions further lead us to uncover a critical role of globally maintained cellular polarity in form of an axial budding pattern in lifespan regulation, the functional significance of which thus far remained essentially unexplored. We also find that the asymmetric localization of Mfb1p depends on potentially novel structures of the actin cytoskeleton and the loss of Mfb1p-polarization with age may accurately predict remaining cellular lifespan.

Introdução: A humanidade passa por uma transformação notável, com profundas implicações para a organização social e para as políticas de Saúde Pública: o envelhecimento da população. Ao contrário dos países desenvolvidos, no Brasil e na maioria dos países em desenvolvimento a população idosa vem aumentando em um cenário de pobreza e despreparo. Nesse quadro, os idosos com idade igual ou superior a 80 anos – que, segundo o Censo de 2000, já eram 1.787.607 - representam um segmento da população pouco estudado e que possui demandas e características singulares e notavelmente diferentes das dos idosos mais jovens. Objetivo: Este estudo, parte do projeto SABE, tem como objetivo descrever as características sócio-demográficas e de saúde da população com idade igual ou maior que 80 anos residente no Município de São Paulo e que participou do estudo SABE no ano de 2000. Metodologia: Essa pesquisa é parte do Estudo SABE – Saúde, Bem-estar e Envelhecimento -, estudo multicêntrico que busca traçar o perfil dos idosos na América Latina e Caribe. A população de estudo foi composta pelos 2136 idosos residentes, no ano de 2000, na área urbana do município de São Paulo, e os dados coletados por meio de questionário padronizado. Resultados: observou-se uma alta prevalência de doenças crônicas não-transmissíveis nessa população, com destaque para a hipertensão e doenças reumáticas. A dentição, a audição e a visão foram mal avaliadas pelos muito idosos entrevistados, e as quedas, realidade para 40,7% dessa população, foram sérias o suficiente para demandarem atendimento médico em 40,2% dos casos. Apesar da fragilidade funcional, uma alarmante proporção de indivíduos não obtinha tratamento adequado e acesso a medicamentos, mesmo para enfermidades graves, como diabetes, doenças cardíacas e cerebrovasculares. Além disso, esses idosos careciam de adequada assistência de cuidadores, mesmo em atividades básicas, como ir ao banheiro ou locomoção. Esse quadro é agravado pelo fato de 14% dos homens e ¼ das mulheres viverem sozinhos, sendo esse o arranjo domiciliar no qual os níveis de ajuda foram os menores encontrados. Os muito idosos brasileiros encontraram-se marginalizados por órgãos públicos e assistenciais despreparados para atender às necessidades especiais desse segmento. Descritores: envelhecimento, muito idosos / Introduction: The world has suffered a remarkable change over the last years with profound implications for Public Health policies: the ageing of its population. Differently from what has happened in developed countries, population ageing in Brazil - and in most developing countries – has taken place in an extremely challenging environment, in which wealth inequalities and inefficient health care systems are the rule. The oldest old are at grater risk because it is an age group that demands very specialized care. Objective: to describe the population aged 80 or over in the Municipality of São Paulo, Brazil, in the year 2000. Methods: this research is part of the SABE study (Helth, Well Being and Ageing), which aims to describe the elderly population in Latin America and the Caribbean. Subjects were 2136 elderly people in São Paulo, Brazil. A standardized questionnaire was used to collect the data. Results: there was a high prevalence of chronic diseases, especially hypertension and rheumatic diseases. Hearing and dental health were badly assessed; 42% had suffered at least one fall and 40,2% of these falls were serious enough to demand medical assistance. Despite the high prevalence of morbidities, a high proportion of the population – sometimes greater than 50% - did not have medicines or appropriate treatment for these diseases, being many of them as serious as heart diseases or diabetes. They also lacked assistance from caregivers, even for basic activities of daily living, such as moving around in the house or using the bathroom. 14% of the elderly men and 25% of the elderly women aged 80 or over lived alone, and this was the worst living arrangement when it comes to receiving help from caregivers. The future for the oldest old living in the Municipality of São Paulo looks bleak, and urgent measures have to be taken in order to prevent the health care system from collapsing.

Age-related Macular Degeneration (AMD) is the leading cause of irreversible vision loss among elderly people in developed countries. The non-neovascular or “dry” form of AMD accounts for 85%, whereas the neovascular or “wet” accounts for 15%, of all cases. There are no effective treatments for dry AMD mainly because the molecular mechanisms that lead to the development and progression of AMD are not fully understood. Similarly, while wet AMD is being treated with antibodies against vascular endothelial growth factor (VEGF), the underlying cause that results in the development of wet AMD remains elusive. Cytosolic accumulation of nuclear-DNA (nDNA) fragments has been found to trigger inflammation and mediate the development of multiple diseases. Because inflammation plays a pivotal role in AMD pathogenesis, we thus investigated if accumulation of cytosolic nDNA also contributes to AMD. Our data show that cytosolic nDNA is enriched in macular retinal pigment epithelium (RPE) cells of AMD patients. To study the effect of cytosolic nDNA on RPE cells, we mimicked this pathology by deleting the lysosomal endonuclease Dnase2a, which is responsible for degrading DNA fragments, using CRISPR/Cas9. This resulted in cytosolic accumulation of nDNA in cultured primary human RPE cells as well as in the RPE cell line ARPE-19. Importantly, both RPE cell types with Dnase2a loss became senescent and secreted higher levels of VGEF and pro-inflammatory cytokines compared to control. These effects were mediated by the DNA sensor STING and mTOR pathway. Additionally, similar to other senescent cells, these senescent RPE cells secreted factors that acted in a paracrine manner turning otherwise healthy RPE cells into senescent cells that start secreting VEGF as well as pro-inflammatory cytokines. Finally, we found that mice with Dnase2a deletion develop features of AMD-like retinopathy, including drusen- like deposits, thickened Bruch’s membrane, RPE damage, photoreceptor atrophy, and reduced electroretinogram. The pleiotropic downstream effects of cytosolic accumulation of nDNA in RPE cells, which are consistent with the complex AMD pathology, suggest that this phenomenon contributes to the pathogenesis of AMD and thereby opens new opportunities for therapeutic interventions. / 2020-10-24T00:00:00Z

With ageing comes a deterioration of metabolic and physiological changes that often manifest themselves as age-related comorbidities. Calorie restriction (CR) is a robust intervention that can prevent and reverse such changes, resulting in reduced ageassociated disease and increased lifespan across a wide range of species. Moreover, a link between the extent of restriction and increased lifespan has also been established. Though widely studied, the mechanisms behind the beneficial effects of CR have yet to be fully understood. Consequently, I investigated metabolomic changes in the liver, plasma, brown adipose tissue (BAT) and cerebellum in five month old male C57BL/6 mice undergoing three months of either 10, 20, 30 or 40% CR, in addition to 12 hour and 24 hour ad libitum fed groups. Behavioural, physiological and molecular data was collected on each individual mouse and I used this information, in addition to my own metabolomic data to determine associations between phenotypic changes with graded CR. My results indicate that increasing CR resulted in greater numbers of significantly differentiated metabolites across all four tissues, and these were related to changes across sphingolipids, carnitines, bile acids, vitamins and amino acids. Metabolic remodelling in the liver indicated a shift from lipogenesis to lipolysis and changes in the plasma indicated an increase in absorption of vitamins from the stomach and colon. Changes in neurotransmitters and their precursors suggested activity and temperature driven BAT activation, in addition to an increase in antioxidant power, this was also seen in the cerebellum where metabolites associated with signalling in the hypothalamus were increased in a graded fashion with CR. In all tissues changes were linked with behaviours that accompany hunger signalling such as increased food anticipatory activity and reduced body temperature. Together, these changes reflect multi-tissue beneficial effects of CR, which may function to alleviate age-related comorbidities.

Healthy ageing in the absence of neurodegenerative diseases is accompanied by a variety of perceptual and cognitive changes, which can occur on various distinct but interacting levels. Much research has been devoted to understanding how basic cognitive functions such as working memory and attention change with age. However, only more recently has age-related changes on perceptual functions been investigated, thus less is understood. My thesis aimed to address this gap in the literature, by exploring age-related changes in visual perception, specifically changes related to visual temporal processing of natural and biological stimuli. Also investigated was the extent to which age-related changes in perceptual and cognitive functions affect each other. Chapter 2 investigated the time course of visual processing of natural scene categorisation in older adults. Younger, young-old and old-old adults performed a go/no-go task, in which they had to respond to images of animals whilst ignoring images of landscapes. The results showed that the temporal processing of complex scenes is impaired in healthy older adults and this deficit becomes more pronounced with increasing age. Chapter 3 assessed whether age-related decline in biological motion perception is mediated by impaired attentional abilities. Younger and older adults performed a series of tasks assessing biological motion perception and visual attention. The results indicated that age-related changes in biological motion perception are not driven by general attentional decline. Lastly, Chapter 4 explored whether age-related changes in biological motion and attention tasks can be explained by differences in the allocation of attention. Younger and older adults performed a conjunctive visual search, and two biological motion tasks, while their eye movements were being tracked. The results illustrated that differences in the allocation of attention cannot explain age-related differences found on biological motion perception and attention tasks. Overall, my findings provide substantial evidence to suggest that both visual perceptual and cognitive abilities change with healthy ageing. However, my results also indicate that certain aspects of these two functions remain relatively preserved in older adulthood.

The transcription factor p53 is an important master regulator of the cellular response to stress. Mdm2 is an E3 ubiquitin ligase that is the primary negative regulator of p53. Mdm2 downregulates p53 activity through three mechanisms: proteasome-mediated degradation, exportation from the nucleus, and direct inhibition through binding. Though the roles of the Mdm2-p53 axis in cancer have been well characterized, the relationship between p53 and other diseases remain elusive. Recently, three novel Mdm2 mutations were identified in patients with premature aging. One mutation leads to the abolishment of the Mdm2 stop codon, thereby extending the Mdm2 C-terminus by five additional amino acids. The other mutation leads to alternative splicing of Mdm2, resulting in two isoforms: a full length Mdm2 protein with a point mutation in the p53 binding domain and a truncated Mdm2 protein that has a 25 amino acid deletion in the p53 binding domain. Our results indicate that the causative Mdm2 variants are hyper-stable and lead to increased p53 protein stabilization. The anti-terminating mutant Mdm2 is defective as an E3 ligase, but retains its ability to bind and dampen p53 activity. However, p53 can be hyper-activated upon induction. Analysis of patient fibroblasts, patient lymphoblastoid cell lines, and genome-edited cells that express mutant Mdm2 confirmed the aberrant regulation of p53. MdmX may also potentially play a compensatory role in this axis. Altogether, our results demonstrate that defective Mdm2 can lead to constitutive dysfunctional regulation of p53 and contribute to accelerated aging phenotypes.

Purpose: The proportion of the United States population in older adulthood is growing rapidly, and with that growth comes an increase in diseases such as cancer. As rates of illness increase, there is a concomitant increase in cognitive and psychological correlates of illnesses like cancer. There is evidence that some cancer treatments, particularly chemotherapy, affect cognition for cancer patients, although these results are inconsistent. Additionally, depression, and other health factors such as activities of daily living (ADLs) have been found to relate to cognitive impairment among older adults with cancer. Method: The current study used latent class growth analysis (LCGA) to explore longitudinal data from the Health and Retirement Study. The primary goal was to investigate possible trajectories of cognitive functioning in older adults diagnosed with, and surviving cancer. Possible psychological, health, and demographic predictors of membership in these cognitive trajectories were investigated. Results: Findings indicated that three classes of cognitive functioning best fit the data: these were High Recall, Middle Recall and Low Recall Classes, which represented fairly stable trajectories from pre-diagnosis to a period four years later. Various covariates of class membership were included in the analyses. Treatment with chemotherapy significantly predicted membership in the High Recall Class, however this finding is accounted for by an interaction with younger age. More symptoms of depression after diagnosis (but not prior to diagnosis) were significantly predictive of membership in the Low Recall Class. A higher self-reported probability of living to the age of 85 pre-diagnosis predicted membership in the High Recall Class, and greater difficulty with ADLs post-diagnosis predicted membership in the Low Recall Class. Finally, individuals in the High Recall Class were significantly more likely to be younger, female, and more highly educated, when compared to both the Middle and Low Recall Classes. Limitations: The current study is limited by the wide spacing of data collection and dearth of sensitive and varied measures of cognitive functioning, which in turn limits the generalizability and specificity of the findings. Additionally, a lack of data on cancer type, staging and treatment variables make more nuanced analysis difficult. It is not possible to generalize these findings to individuals who passed away within two years of their diagnosis, not to individuals of minority status, who were underrepresented in this sample. Conclusions: These results may inform the understanding of cognitive functioning in older adults surviving cancer, as it relates to psychological, demographic and other health factors, with implications for timing and targeting of interventions.

Estima-se que, no ano de 2025, 23% da população total dos países desenvolvidos estarão com mais de 60 anos, evidenciando-se assim o envelhecimento gradativo do contingente populacional destes países. Deste modo, é perceptível o contingente de mulheres que estarão vivenciando a fase da menopausa com seus efeitos biológicos, psicológicos e sociais. As mudanças hormonais e fisiológicas que acontecem nas mulheres durante a fase da menopausa, acompanhadas pela desvalorização estética do corpo e por toda uma sintomatologia física e psíquica, têm sido interpretadas como perda da feminilidade, sinalizando o envelhecimento inevitável e a finitude. No entanto, muitos dos desconfortos que as mulheres vivenciam nesta fase não se devem às mudanças biológicas, mas ao seu processo de socialização, caracterizando a influência de gênero. Neste contexto, este trabalho teve como objeto o estudo da influência da relação de gênero na vivência e no significado do processo da menopausa, tendo como objetivos: descrever a vivência da menopausa a partir da perspectiva de mulheres que a vivenciam e identificar as particularidades relacionadas ao gênero diretamente envolvidas na experiência da menopausa a partir da perspectiva das mulheres. Para desenvolvimento do trabalho foi utilizada abordagem qualitativa de natureza descritiva com vinte mulheres de idade entre 45 e 55 anos que apresentavam menopausa espontânea e eram clientes das Unidades Básicas de Saúde da cidade de Curitibanos-SC, no período de 1 a 15 de outubro de 2009. Para a coleta de dados foi utilizado um roteiro de entrevista semi-estruturada com uma questão norteadora: Fale-me como é para você estar vivenciando a menopausa. A interpretação e análise foram feitas através de análise de conteúdo do tipo temática descritas por Bardin. Nas narrativas, identificaram-se categorias que foram integradas em quatro temas principais: 1- Vivenciando a Menopausa, 2- Identificando Transformações no Corpo e na Vida, 3- Cuidando de Si, 4- Buscando Informações/Influências e Construindo Conhecimento. Foi possível identificar nessas categorias que as mulheres trazem o conceito de que a fase da menopausa é uma doença, e relacionam essa fase com envelhecimento e declínio físico, a qual traz grandes sofrimentos, o que demonstra a influência de gênero no vivenciar desta fase. As entrevistadas explicitaram em suas falas diversos sintomas que as incomodavam e interferiam em suas atividades diárias e na sua maneira de ser, repercutindo muitas vezes no seu comportamento familiar e profissional. O conhecimento sobre a menopausa, neste grupo de mulheres, foi construído ao longo de suas vidas e reflete as suas realidades culturais e sociais, deixando evidente a escassez de fontes de informação e os tabus relacionados com relação à fase da menopausa. Este estudo contribui com a geração de conhecimentos levando em consideração os efeitos que a influência de gênero pode ter na vivência e percepção da menopausa, desmistificando-a para que a vivência das mulheres durante esse período não seja condicionada por estereótipos e crenças relacionadas ao gênero. / They is esteem that, in the year of 2025, 23% of the total population of the developed countries will be with more than 60 years, what show the gradual aging of the population contingent of these countries. In this way, is perceivable the contingent of women who will be living deeply the phase of the menopause with its biological, psychological and social effects. The physiological and chemical changes that happen in the women during the menopauses phase, followed with the aesthetic depreciation of the body, have been interpreted as loss of the feminine characteristics, signaling the inevitable aging and the end of the life. However, many of the discomforts that the women live in this phase not must to the biological changes, but to its process of socialization, characterizing the genre influence. In this context, this work had as object the study of the influence of the relation of genre in the experience and meaning of the process of the menopause, having as objective: describes the experience of the menopause on the perspective of women and to identify the particularitities involved to the genre in the experience of the menopause on the perspective of the women. For development of the work, was done one research descriptive-qualitative with customers of the Basic Units of Health of the city of Curitibanos-SC, in the period of 1 to 15 of October of 2009. For the collect of data, one structured interview was used with an orienting question: It speaks to me, as are for you to be living the menopause. The interviews were done with 20 women, with ages between 45 and 55 years and that had presented spontaneous menopause. The interpretation and analysis was done by analysis of content of the thematic type described by Bardin. In the narratives, was done the identification of categories that was integrated in four main subjects: Living on the Menopause, Identifying Transformations in the Woman Body and in the Life, Cares Myself, Research Information/Influences and Building Knowledge. Was possible identify in these categories that the women bring the idea that the menopause phase is an illness, and relate this phase with aging and physical decline, which brings great sufferings, what it demonstrates to the influence of genre in this phase. The interviewed ones showed diverse symptoms in its words, this bother and intervene in the daily activities and its way to be, and this situation, influence many times in its familiar and professional behavior. The knowledge on the menopause, in this group of women, was constructed throughout its lives and reflects its cultural and social realities, leaving evident the scarcity of information sources and the taboos related with the subject. This work improve of knowledge in consideration the effect that the genre influence can have in the experience and perception of the menopause, demystifying the experience mode of the women during this period, not conditioning for stereotypes and beliefs related to the genre.

朱穎. / "2010年8月". / "2010 nian 8 yue". / Thesis (M.Phil.)--Chinese University of Hong Kong, 2010. / Includes bibliographical references (leaves 91-100). / Abstracts in Chinese and English. / Zhu Ying. / 目錄 --- p.3 / 論文摘要 --- p.5 / Abstract. --- p.6 / Chapter 第一章 --- 引言 --- p.7 / Chapter 1.1 --- 文獻綜述 --- p.10 / Chapter 1.1.1 --- 子女分工 --- p.10 / Chapter 1.1.2 --- 性別分工 --- p.11 / Chapter 1.1.3 --- 理想和現實的落差 --- p.13 / Chapter 1.1.4 --- 老人的養老期望 --- p.15 / Chapter 1.2 --- 研究方法 --- p.15 / Chapter 1.3 --- 文章架構 --- p.22 / Chapter 第二章 --- 當代農村家庭中的養老內容 --- p.25 / Chapter 2.1 --- 經濟支持 --- p.26 / Chapter 2.1.1 --- 農村家庭中老人的收入來源 --- p.26 / Chapter 2.1.2 --- 農村家庭中老人的經濟需求 --- p.28 / Chapter 2.2 --- 日常照料 --- p.29 / Chapter 2.2.1 --- 老年父母的需求 --- p.29 / Chapter 2.2.2 --- 農村養老與社區情理 --- p.32 / Chapter 2.2.3 --- 居住安排 --- p.34 / Chapter 2.2.4 --- 由現今居住棋式所引發的養老問題 --- p.37 / Chapter 2.3 --- 情感支持 --- p.40 / Chapter 2.3.1 --- p.40 / Chapter 2.3.2 --- "“主與我同在"" " --- p.44 / 討論 --- p.45 / Chapter 第三章 --- 子女間的分工合作 --- p.46 / Chapter 3.1 --- 養老分工的傳統理想模式 --- p.46 / Chapter 3.2 --- 養老分工理想模式的轉變 --- p.55 / Chapter 3.3. --- 理想與現實的落差 --- p.59 / Chapter 3.3.1 --- 當女兒付出了比期望多 --- p.59 / Chapter 3.3.2 --- 子女出去打工了 --- p.63 / 討論 --- p.66 / Chapter 第四章 --- "“家庭策略與家庭迷思""一家庭關係中的權力變動與日常生活中的協商 " --- p.67 / Chapter 4.1 --- 性別策略 --- p.68 / Chapter 4.1.1 --- "對“男女平等""的解釋:""耙子""和""匣子""" --- p.68 / Chapter 4.1.2 --- """考順女兒""的權力象徵" --- p.70 / Chapter 4.1.3 --- 兒媳的策略:利益結盟和自立門派 --- p.73 / Chapter 4.2 --- 留守子女:依照空間劃分的責任 --- p.75 / Chapter 4.3 --- 父母的策略 --- p.77 / Chapter 4.2.1 --- "“息事寧人""的矛盾調停者 " --- p.77 / Chapter 4.2.2 --- "“兇狠的兒媳,懦弱的兒子"" " --- p.78 / Chapter 4.2.3 --- 自力更生的態度 --- p.79 / Chapter 4.2.4 --- 風險分擔:參與保險 --- p.81 / 結論 --- p.81 / Chapter 第五章 --- 結論 --- p.83 / 參考文獻 --- p.91