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Phosphatidylserine exposure in red blood cells from patients with sickle cell diseaseCytlak, Urszula Malgorzata January 2015 (has links)
No description available.
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Asociación entre síntomas depresivos en la madre y anemia en niños de 6 a 59 meses de edad en PerúAlarcon Guevara, Samuel Leonardo 27 November 2018 (has links)
Antecedentes: Aproximadamente 01 de cada 03 niños peruanos de 6 a 59 meses de edad tiene anemia. Por otro lado, la depresión materna, que puede ser incapacitante y afectar el cuidado adecuado de los niños, está asociada con la desnutrición crónica de los mismos. De igual manera, la desnutrición crónica está asociada a anemia en los niños. Por esto, el objetivo de este estudio es evaluar si existe asociación entre los síntomas depresivos de las madres y la presencia de anemia en sus hijos. Métodos: Estudio transversal analítico de la Encuesta Demográfica y de Salud Familiar 2015, que es representativa a nivel nacional. Los síntomas depresivos se midieron con el Patient Health Questionnaire -9 (PHQ-9) utilizando una puntuación de 10 como punto de corte. La presencia de anemia se midió con HemoCue® y se consideró positiva cuando la hemoglobina era inferior a 11 g / dL. La razón de prevalencia cruda y ajustada (RP y RPa) se calcularon con intervalos de confianza del 95%, utilizando modelos lineales generalizados de la familia Poisson Resultados: Se analizaron 6683 binomios madre-hijo. La prevalencia de anemia en los niños fue del 28,7% [IC 95%: 27,3-30,2]. y la de los síntomas depresivos en la madre de 6.9% [IC 95%: 6,1-7,9]. No se encontró asociación estadísticamente significativa entre las dos variables principales en el análisis bivariado ni en los diferentes modelos multivariados (RPa: 1.05, IC 95%: 0.85-1.30). Nuestros resultados no muestran asociación entre los síntomas depresivos maternos y la anemia en el niño. Conclusiones: No se encontró asociación entre síntomas depresivos maternos y anemia en sus hijos mayores de 6 a 59 meses en el análisis bivariado, multivariado, gráfico de dispersión o con análisis por estratos. La prevalencia de síntomas depresivos maternos y de anemia en el niño se mantienen elevados en la muestra. Se sugiere continuar el estudio de esta posible asociación y el modelo multifactorial de anemia en el niño con estudios prospectivos. / Background: Approximately, one in three Peruvian children aged 6 to 59 months have anemia. Maternal depression, which may be disabling and affect the proper care of children, is associated with chronic malnutrition of their children. In the same way, chronic malnutrition in children is associated with children anemia Therefore, the aim of this study is to evaluate if there is an association between depressive symptoms of mothers with the presence of anemia in their children. Methods: Analytical cross-sectional study of the Peruvian Demographic Health Survey 2015, which is nationally representative. Depressive symptoms were measured with the Patient Health Questionnaire-9 (PHQ-9) using a score of 10 as cut-off. The presence of anemia was measured using HemoCue® and was considered positive when hemoglobin was less than 11 g/dL. Crude and adjusted prevalence ratios (PR and aPR) were calculated with 95% confidence intervals, using generalized linear models of the Poisson family. Results: We analyzed 6683 mother-child binomials. The prevalence of anemia in the children was 28.7% [95% CI: 27.3-30.2]. and that of depressive symptoms in the mother of 6.9% [95% CI:6,1-7,9]. We did not find statistically significant association between the two main variables in the bivariate analysis nor in the different multivariate models (RPa: 1.05, 95% CI: 0.85-1.30). Our results show no association between maternal depressive symptoms and anemia in the child. Conclusions: We found no association between maternal depressive symptoms and anemia in their children aged 6 to 59 months in the bivariate, multivariate, scatter plot or stratum analysis. The prevalence of maternal depressive symptoms and anemia in their child remain high in the sample. We suggest to continue the study of this possible association and the multifactorial anemia model with prospective studies. / Tesis
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Modulação pelo haplótipo Bantu da resposta ao uso de hidroxiureia em anemia falciforme /Okumura, Jéssika Viviani. January 2013 (has links)
Orientador: Claudia Regina Bonini-Domingos / Banca: Nicola Amanda Conran Zorzetto / Banca: Maria Stella Figueiredo / Resumo: A anemia falciforme (AF) apresenta processos fisiopatológicos complexos que dificultam o tratamento e culminam no desenvolvimento de manifestações clínicas diversificadas, as quais são moduladas por marcadores genéticos, como os haplótipos da globina beta S (β S ), e bioquímicos como glicoproteínas plasmáticas detoxificadoras: haptoglobina (Hp) e a hemopexina (Hpx) que formam complexos com a Hb e grupo heme livres, respectivamente, garantindo efeitos antioxidantes e anti-inflamatórios, e a eritropoetina (Epo) que repõe os eritrócitos em resposta à hemólise, por exemplo. Desta forma, o objetivo do trabalho foi verificar, em portadores da AF, a influência do haplótipo Bantu sobre o tratamento com hidroxiuréia (HU) por meio da análise de marcadores de estresse oxidativo e eritrogênico, correlacionando-os com parâmetros hematimétricos e níveis de hemoglobina fetal (Hb F). Para isso, o grupo amostral (67 portadores da AF), foi dividido em três subgrupos: 25 (37,3%) homozigotos para o haplótipo Bantu (Bantu/Bantu), 26 (38,8%) heterozigotos para o haplótipo Bantu (Bantu/_) e 16 (23,0%) sem o haplótipo Bantu (_/_). Todos sob uso de HU há pelo menos 300 dias, sem transfusão sanguínea há 120 dias e adultos independentes do gênero. A confirmação da homozigose para Hb S e dos haplótipos β S , foi realizada por PCR-RFLP; os níveis de peroxidação lipídica (TBARS) foram avaliados por método espectrofotométrico e as glicoproteínas plasmáticas (Epo, Hp e Hpx) por ensaio imunoenzimático. Os parâmetros hematológicos foram fornecidos pelo HEMORIO. A média de Hb F no grupo amostral foi de 12,1% e não diferiu entre os subgrupos (p=0,55), porém o Bantu/_ apresentou acréscimo na média sendo o Benin o segundo haplótipo mais frequente. Os valores médios de... (Resumo completo, clicar acesso eletrônico abaixo) / Abstract: Sickle cell anemia (SCA) presents complex pathophysiological processes that complicate the treatment and culminate in development of diverse clinical manifestations, which are modulated by genetic markers, such as beta-S globin haplotype (β S ), and biochemical, such as plasmatic glycoproteins with action to detoxify: haptoglobin (Hp) and hemopexin (Hpx), which form complexes with free Hb and heme, respectively, providing antioxidant and anti-inflammatory effects, and erythropoietin (Epo), that restores the erythrocytes in response to hemolysis, for example. Thus, the aim of this study was to determine, in patients with SCA, the influence of Bantu haplotype in treatment with hydroxyurea (HU), by analysis of oxidative stress and erythrogenic markers, correlating them with hematological parameters and levels of fetal hemoglobin (Hb F). For this, the sample group (67 SCA patients), was divided into three subgroups: 25 (37.3%) were homozygous for the Bantu haplotypes (Bantu/Bantu), 26 (38.8%) heterozygous for the Bantu haplotype (Bantu/_) and 16 (23.0%) without the Bantu haplotype (_/_). The sample group was under use of HU for at least 300 days, without blood transfusion for 120 days and were all adults independent of the genus. The confirmation of homozygous Hb S and β S haplotypes was performed by PCR-RFLP; levels of lipid peroxidation (TBARS) were measured by the spectrophotometric method and plasmatic glycoproteins (Epo, Hp and Hpx) by enzyme immunoassay. Hematological parameters were provided by HEMORIO. The average of Hb F in the sample group was 12.1% and did not differ between the groups (p = 0.55), but the Bantu/_ showed an increase in the average, being Benin the second most frequent haplotype. The average values of Epo, Hp and Hpx did not differ between the subgroups, indicating... (Complete abstract click electronic access below) / Mestre
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Correlación de parámetros clínicos y de laboratorio con resultados materno-neonatales en pacientes con diagnóstico del Síndrome de Hellp, en el Hospital Nacional Docente Madre Niño San Bartolomé, durante los años 2000-2003Luque Mamani, Renán January 2004 (has links)
Objetivo General: Determinar la correlación clínica y de exámenes de laboratorio del Síndrome Hellp con los resultados materno - neonatales.
Material y Métodos: Se realizó un estudio observacional longitudinal, retrospectivo correlacional, en pacientes como diagnostico Síndrome de Hellp, encontrándose en un lapso de tres años, un total de 153 casos; se correlacionó los resultados maternos y resultados neonatales con los parámetros de laboratorio que hacen el diagnóstico de Sd de Hellp. Para el análisis se usaron las pruebas t de student, para las variables paramétricas; y para las variables no paramétricas, se utilizó la prueba de Kruskal – Wallis, considerándose la significación estadística con un valor de p <0.05.
Resultados: La incidencia del S. de Hellp fue de 0.76%, siendo la clase III la mas frecuente (60%), el 48% no tuvo CPN, la mayor parte de las gestaciones culminaron por cesárea (62.7%), siendo la clase I la que tuvo mas cesáreas; se observó que el edema pulmonar se encontró en la clase I, los vómitos están relacionados significativamente con malos resultados materno y neonatales, La epigastralgia se relaciona mejor con la clase I en el 50%, la eclampsia no guarda relación de causalidad con los niveles plaquetarios, los parámetros de laboratorio no guardan relación con resultados neonatales, la diferencia de resultados neonatales con el tipo de Hellp no fue observada, los preterminos se presentaron en el 44.3%, la ictericia se asocio mas a clases I y II con 50 y 41%, la mortalidad neonatal se observo mas con la clase 1 con 16.7%; en los RN se observo la relación directa de nauseas con muerte neonatal 25%, también de nauseas con sepsis neonatal (66.6%), finalmente se aprecia un mayor uso de hemoderivados en la Clase I, >66%.
Conclusiones: La Clase de Hellp de mayor severidad se correlaciona con malos resultados maternos, mayor tasa de cesáreas, uso de hemoderivados, complicaciones oculares, pulmonares, uso de UCI, mayor tiempo de hospitalización, así como de malos resultados neonatales, sepsis y muerte neonatal; siendo el parámetro clínico de mayor importancia las nauseas y los vómitos, que se relacionan con malos resultados maternos; en cuanto a los niveles de plaquetas, los valores bajos se asocian a muerte y sepsis neonatal. / Tesis de segunda especialidad
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Relaciʹion entre anemia, consumo de hierro y parasitosis intestinal en niños menores de cinco años pertenecientes a las etnias indʹigena, mestiza y negra del ʹarea rural de Imbabura 1999 /Paguay Ruiz, R. Patricio. January 2000 (has links)
Thesis (Licenciado en Nutriciʹon y Dietʹtica)--Universidad Tʹecnica del Norte. Facultad Ciencias de la Salud Escuela de Nutriciʹon y Dietʹeica. / Abstract in Spanish and English.
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Differential cytokine mRNA expression induced by binding of virulent and avirulent molecularly cloned equine infectious anemia viruses to equine macrophagesLim, Wah-Seng 15 November 2004 (has links)
Equine infectious anemia virus (EIAV) causes rapid development of acute disease followed by recurring episodes of fever, thrombocytopenia and viremia, termed chronic EIA. Most infected horses control the virus by immune mechanisms and become inapparent carriers. To further our understanding of the equine immune response to EIAV, a multi-probe ribonuclease protection assay (RPA) was developed to quantitate equine-specific cytokine mRNAs. Eleven template plasmids specific to ten equine cytokine genes and the ?-actin gene were generated, from which radiolabeled anti-sense RNA probes were produced. The RPA simultaneously quantitated mRNA levels of interleukin (IL)-1, IL-1, IL-6, IL-8, IL-10, IL-12 p35, IL-12 p40, interferon (IFN)-, transforming growth factor (TGF)-1 and tumor necrosis factor (TNF)- in equine peripheral blood mononuclear cells and equine monocyte-derived macrophages (EMDM). The assay detected as few as 5105 RNA molecules and displayed coefficients of variation of 0.03-0.08 when normalized to -actin expression. Using this RPA, cytokine expression in EMDM infected with 2 molecularly cloned viruses (EIAV17 and EIAV19) was determined. EIAV17 varies from EIAV19 only in env, rev and LTR and causes fatal disease in Shetland ponies. When added to EMDM cultures, virulent EIAV17 stimulated expression of IL-1, IL-1, IL-6, IL-10 and TNF-. These cytokine mRNAs were significantly elevated by 0.5 to 1 hr post infection (hpi) and returned to basal levels by 12 to 24 hpi, indicating modulation by early event(s), such as receptor binding. In contrast to EIAV17, EIAV19 is avirulent in vivo and failed to induce any of the tested cytokines in EMDM. These data show a direct correlation between the virulence of the EIAV clone and the induction of cytokines. The cytokines stimulated by EIAV17 may contribute to EIA-associated symptoms, enhance viral replication in the host, and regulate the host immune response. To determine whether cytokine induction requires EIAV17 replication, EMDM cultures were exposed to UV-inactivated EIAV17 and cytokine induction was monitored. UV-inactivation did not block cytokine induction by EIAV17, suggesting dispensability of viral replication. Given that EIAV17 induces cytokines in a rapid and replication-independent manner, the activation of cytokine expression is likely mediated by binding of EIAV17 to equine macrophage receptor(s).
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Effectiveness of Mandated Folic Acid and Iron Fortification of Wheat Flour on Anemia in Children of Ivory CoastBiemi, Flinle Danielle 11 May 2013 (has links)
Purpose: Anemia in children is a major Public Health problem in developing countries such as Ivory Coast. The fortification of wheat flour with iron and folic acid has been mandated in this country in 2007. To date, there are no studies conducted to assess the effectiveness of these fortification efforts. Therefore, the purpose of this study was to determine if mandated iron and folic acid fortification of wheat flour has reduced the prevalence of anemia in Ivory Coast children.
Methods: The study was conducted at the pediatric unit of the university hospital of Treichville, one of the 3 main hospitals of Abidjan, the largest city of Ivory Coast. The medical records of 467 children from 5 to 14 years old were analyzed. The period from January 1st 2004 to December 31st 2006 was considered as pre-fortification period and the period from January 1st 2008 to December 31st 2010 was regarded as the post-fortification period. Data for anemia, hemoglobin, hematocrit, red blood cell count, and MCV were compared between pre- and post-fortification periods.
Results: The results showed that there were no statistically significant difference in prevalence of anemia and mean hemoglobin, hematocrit, red blood cells, and MCV between pre and post-fortification periods.
Conclusion: The double fortification of wheat flour with folic acid and iron had no significant impact on anemia in Ivorian children. This is perhaps due to the fact that wheat flour is not widely consumed as rice and rice products are more popular in Ivory Coast population. The Ivorian government perhaps should focus efforts on the fortification of these products.
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Happy Fish: A Novel Iron Supplementation Technique to Prevent Iron Deficiency Anemia in Women in Rural CambodiaCharles, Christopher Vaughn 14 May 2012 (has links)
Maternal and child undernutrition are a significant problem in the developing world, with serious consequences for human health and socio-economic development. In Cambodia, 55% of children, 43% of women of reproductive age, and 50% of pregnant women are anemic. Current prevention and control practices rely on supplementation with iron pills or large-scale food fortification, neither of which are affordable or feasible in rural Cambodia. In the study areas, 97% of women did not meet their daily iron requirements. The current research focuses on the design and evaluation of an innovative iron supplementation technique. A culturally acceptable, inexpensive and lightweight iron ingot was designed to resemble a fish species considered lucky in Khmer culture. The ingot, referred to as ‘try sabay’ or ‘happy fish’, was designed to supply iron at a slow, steady rate. Iron leaching was observed in water and soup samples prepared with the iron fish when used concurrently with an acidifier. More than 75% of daily iron requirements can be met with regular use. Its use in the common pot of soup or boiled water provides supplementation to the entire family. The effectiveness of the iron fish was investigated in a randomized community trial involving 310 women in rural Cambodia. Blood samples were taken at baseline and every three months thereafter, over a 12-month trial period. Significant increases in hemoglobin concentrations were observed in women allocated an iron fish when compared to controls throughout the study, with an endline difference of 11.6 g/L. Significant improvements in serum ferritin concentration were observed at 9 months (6.9 ng/mL) and endline (30.8 ng/mL) in women who used an iron fish regularly when compared to the control group. Overall, use of the iron fish led to a two-fold reduction in the prevalence of anemia. The supplement was used daily by 94% of the households at the end of the trial. The study highlights the acceptability and effectiveness of a fish-shaped iron ingot as a means of improving dietary iron content. It offers a promising, simple solution to iron deficiency anemia if the project can be scaled-up for use throughout the country. / Canadian Institutes of Health Research, University of Guelph, International Development Research Centre (Canada)
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Understanding the Role of Ribosomal Proteins and Aberrant FLVCR1 Splicing in Diamond Blackfan AnemiaFernandes, Abigail Brenda 21 March 2012 (has links)
Diamond Blackfan Anemia is a rare congenital disease that is primarily characterized by reduced erythroid progenitors. DBA pathogenesis has been associated with genes encoding ribosomal proteins (RPs) which are important in translation. However, this fails to explain why erythropoiesis is specifically disrupted. Our lab previously found that aberrant splicing of the human transcript encoding heme exporter, FLVCR1, is involved in DBA pathogenesis; and that RPS19 implicated in 25% of DBA patients, regulates FLVCR1 transcript splicing.
This thesis investigated the role of another DBA associated gene encoding RPS17, in the regulation of FLVCR1 splicing and disrupted erythropoiesis in DBA. My findings further support the role of FLVCR1 aberrant splicing in DBA and provide evidence suggesting that RPS17 may not be a candidate DBA gene. Furthermore, my study implicates a potential role for RPS19 transcript levels in defective erythroid differentiation observed in DBA.
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Understanding the Role of Ribosomal Proteins and Aberrant FLVCR1 Splicing in Diamond Blackfan AnemiaFernandes, Abigail Brenda 21 March 2012 (has links)
Diamond Blackfan Anemia is a rare congenital disease that is primarily characterized by reduced erythroid progenitors. DBA pathogenesis has been associated with genes encoding ribosomal proteins (RPs) which are important in translation. However, this fails to explain why erythropoiesis is specifically disrupted. Our lab previously found that aberrant splicing of the human transcript encoding heme exporter, FLVCR1, is involved in DBA pathogenesis; and that RPS19 implicated in 25% of DBA patients, regulates FLVCR1 transcript splicing.
This thesis investigated the role of another DBA associated gene encoding RPS17, in the regulation of FLVCR1 splicing and disrupted erythropoiesis in DBA. My findings further support the role of FLVCR1 aberrant splicing in DBA and provide evidence suggesting that RPS17 may not be a candidate DBA gene. Furthermore, my study implicates a potential role for RPS19 transcript levels in defective erythroid differentiation observed in DBA.
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