Shortfin eels (Anguilla australis) and longfin eels (A. reinhardtii) are true freshwater eels of the genus Anguilla. There are many mysteries still unsolved for the freshwater eel lifecycle, such as location of the spawning grounds, conditions that promote metamorphosis from the leptocephalid to glass eel phase, and the mechanisms that affect glass eel recruitment. In Australia, little is also known about the estuarine habitats of glass eels as they migrate towards freshwater, and the age at which these eels enter estuaries. Both species are of commercial importance in the estuary fishery where they are caught in eel traps for export. There is also a small, but potentially lucrative, aquaculture industry for ongrowing glass eels to market demand size. This thesis investigates the spatial and temporal recruitment of both species of glass eels to estuaries within NSW, the habitats that may be of importance to them as they continue their upstream migration, and the age at which these eels entered the estuaries. Firstly, a new sampling device needed to be developed since conventional methods to catch glass eels often required constant observation of gear, multiple operators, specific physical site characteristics, and/or were expensive. The artificial habitat collectors that were developed were then used to sample six estuaries in NSW monthly within one week of the new moon. Shortfins showed a more consistent and defined recruitment across all sites than longfins, where the peak shortfin recruitment season was from April - August. Longfins recruited primarily from January - May but often recruited outside of this period. Five year collections at one of these sites provided important recruitment information. It appeared that longfins failed to recruit to this site during 2000/01, which could affect commercial catches of this species when they enter the fishery. The East Australian Current (EAC) probably transports glass eels from spawning sites in the Coral Sea southward to the east coast of Australia but there was no predicted lag time in the recruitment of eels from northern to southern estuaries. Therefore, it was not possible to predict the timing of recruitment of glass eels in one estuary based on the timing of recruitment in another more northern estuary. When glass eels enter estuaries their upstream migration is assisted by the night flood tide. During the ebb tide, glass eels burrow into the substrate and resurface at the next night flood tide. The eels do not select particular habitats at this time, rather, their location is dictated by the tide. However, once glass eels reach the estuarine/freshwater interface, they may prefer more complex habitats such as seagrass/macrophytes or rocks/cobbles in which to hide during the day. At this interface, glass eels undergo a physiological change to adapt to a freshwater existence and this change may take up to a few weeks. During this time, glass eels commonly enter the water column during the night flood tide and may be able to locate more suitable habitats in which to hide during the day. The ages of shortfin and longfin glass eels caught in estuaries were examined both spatially and temporally. As the EAC travels north to south, glass eels recruiting to the southern sites were expected to be older. However, shortfins that recruited to the northern-most site in this thesis were older than at all other sites while there was no difference in the ages of longfins. Also, when the ages of longfins that recruited during the main recruitment period were compared to the ages of longfins that recruited outside of this period, there was no difference in ages. Therefore, the hypothesis that these later recruiting eels may have been caught in an eddy prior to their estuarine arrival has been disproved. The ages of shortfins that recruited in two separate years were significantly different from each other and may be due to shortfins' ability to detrain more easily from the weaker currents that exist at these recruitment periods. Conversely, there was no difference in the ages of longfins that recruited in the same month during three separate years. The estimated hatch dates for shortfins was estimated at October to January, while for longfins, estimated hatch time was July to September for eels that recruited during the peak recruitment period. For longfins that recruited outside of the main recruitment period, estimated hatch times were from December to February. It is unknown, however, whether longfins have an extended spawning period, or whether silver eels arrived at the spawning grounds later and thus produced later arriving longfins. Continuous monitoring of glass eel recruitment to estuaries is necessary to determine whether there are long term declines in the recruitment of Australian eels similar to the declines recently observed for eels in Europe and Asia.

Thesis (M. Phil.)--University of Hong Kong, 2000. / Includes bibliographical references (leaves 68-94).

Thesis (M. Phil.)--University of Hong Kong, 2000. / Includes bibliographical references (leaves 172-216).

No description available.

Depuis plusieurs dizaines d’années, la population des anguilles européennes a souffert d’un dramatique déclin et est classée parmi les espèces hors de leurs limites biologiques depuis 1998. Différents phénomènes, tels que la pêche, les obstacles aux migrations, ou le réchauffement climatique, en sont à l’origine. Cependant, les perturbations environnementales, telles que les contaminations métalliques et organiques ou les épisodes d’hypoxie, participent probablement à la vulnérabilité de cette espèce. Or, l’estuaire de la Gironde est soumis à des contaminations poly-métalliques historiques, ainsi qu’à des contaminations organiques de différentes origines, et à des épisodes hypoxiques réguliers. Les travaux menés au cours de cette thèse, se sont donc composés d’études de terrain visant à identifier les contaminants majeurs chez les anguilles jaunes de l’estuaire de la Gironde, ainsi que leur voie de bio-accumulation et leurs impacts au niveau physiologique, biochimique et moléculaire. Ces études ont nécessité la mise en place d’expériences préliminaires ayant pour objectifs de vérifier les impacts de certaines procédures, telles que l’anesthésie et la mise en cage des anguilles jaunes. D’autre part, les impacts des deux principaux contaminants métalliques et organiques des anguilles européennes de l’estuaire de la Gironde, ont été testés, de façon individuelle et combinée, ainsi que ceux de l'hypoxie sur des anguilles pré-contaminées ou non, lors d’une étude expérimentale.Ainsi, ces travaux ont mis en évidence que les anguilles européennes installées dans la zone avale de l’estuaire, étaient susceptibles d’être soumises à des contaminations poly-métalliques plus importantes, essentiellement d’origine trophique, responsables d’une croissance pondérale moins élevée et de perturbations transcriptionnelles hépatiques et cérébrales. De plus, bien que les contaminations métalliques de ces anguilles ne mettent pas ne danger la santé humaine, les contaminations en PCB sont, en revanche, supérieures aux normes de consommation. D’autre part, ces travaux ont également mis en évidence des dérèglements mitochondriaux ainsi qu’un stress oxydant, au niveau branchial et cérébral chez des anguilles contaminées au Cd, et au niveau cérébral, branchial, hépatique et rénal chez les anguilles contaminées aux PCB. Enfin, la concomitance de ces deux contaminants et/ou d’un épisode d’hypoxie, réduit et/ou retarde les capacités de réponses transcriptionnelles de ces anguilles. Il semble donc que les différentes perturbations chimiques subies par les anguilles européennes au stade jaune au sein de l’estuaire de la Gironde participent de façon non négligeable à la vulnérabilité de cette espèce. / For several decades, the European eel has been suffering from a dramatic decline and has been classified among species beyond their biological limit since 1998. Different phenomena, as fishing, migration barriers, or global warming, are to blame. Environmental perturbations, as metallic and organic contaminations, or hypoxic episodes, probably take part to the vulnerability of this species. The Gironde estuary has been submitted to historic poly-metallic contaminations, to organic contaminations from different origins, and to regular hypoxic episodes. This thesis work is composed of field studies aimed to identify the main contaminants in yellow eel living in the Gironde estuary, their major bioaccumulation way and impacts on physiological, biochemical and molecular parameters. These studies needed preliminary experiments aimed to verify whether some field and handling procedures are consistent with ecotoxicological analyses. Moreover, impacts of the two main contaminants in European eels from the Gironde estuary have been assessed individually or combined, with those of hypoxia on pre-contaminated eels.Thus, these studies have demonstrated that European eels installed in the downstream area of the estuary, were likely to be subject to largest poly-metallic contaminations, mostly by food web, and responsible for a less weight gain and high liver and brain transcriptional disturbances. Furthermore, although the metal contamination of eels does not endanger human health, PCB contaminations are higher than consumption standards. On the other hand, these studies have also revealed oxidative stress and mitochondrial disorders in gills and brain of Cd-contaminated eels, and in brain, gills, liver and kidneys of PCB-contaminated eels. The combination of these two contaminants and/or an episode of hypoxia, reduces and/or delays the transcriptional responses ability of these eels. It seems that the different chemical disturbances, suffered by the yellow eels in the estuary of the Gironde, participate significantly to this species vulnerability.

published_or_final_version / Zoology / Master / Master of Philosophy

Thesis (Ph. D.)--University of Hong Kong, 1994. / Includes bibliographical references (leaves 90-100).

Im Jahr 2006 wurden insgesamt 767 Aale des Bodensee-Obersees auf den Befall mit dem Schwimmblasenwurm A. crassus und dessen Folgen untersucht. Zusätzlich wurden die Befallsdaten von weiteren 2.326 Aalen aus den Jahren 1988 bis 2005 und 2007 bis 2009 ausgewertet. Weiterhin wurden in den Jahren 2006 bis 2008 eine Anzahl von 383 Kaulbarschen und zahlreiche Copepoden auf den Befall mit dem Larvenstadium von A. crassus untersucht. Auf der Ebene der echten Zwischenwirte ließ sich A. crassus im Bodensee-Obersee im Freiwasserplankton nicht nachweisen. Jedoch konnten Copepoden der Gattung C. abyssorum experimentell mit A. crassus infiziert werden. Der Kaulbarsch ist für A. crassus der wichtigste Stapelwirt im Bodensee-Obersee. Die untersuchten Kaulbarsche waren zwischen 18,8% und 52,1% mit A. crassus infiziert, wobei die Infektion einen deutlichen Frühjahrespeak aufwies. Im Jahr 2006 lag die Prävalenz befallener Aale im Bodensee bei 55,6%, die durchschnittliche Befallsintensität lag bei 4,4 adulter Parasiten je Aal. Durch die Datenauswertung von insgesamt 3.425 Aalen konnte der Verlauf der Anguilli­coloidose im Zeitraum von 1989 bis 2009 rekonstruiert werden. Der Höhepunkt der Infektion lag demnach im Jahr 1993 mit einer durchschnittlichen Prävalenz des Erregers von 58,3% und einer durchschnittlichen Befallsintensität von 16,6. Seit 1996 werden die Schadwirkungen von A. crassus auf die Schwimmblase aufgezeichnet und wurden nun in dieser Arbeit ausgewertet. In diesem Zeitraum nahm die Anzahl der Aale mit schweren Schwimmblasenschäden deutlich zu. Im Jahr 2006 besaßen 89,9% der Aale eine Schwimmblase mit geringer bis sehr starker Schädigung. Bei den abwanderungswilligen Blankaalen besaßen insgesamt 10% eine Schwimmblase mit sehr starken Schäden. Es bestand eine positive Korrelation zwischen der Größe der Aale und der Ausprägung der Schwimmblasenschäden. Weiterhin wurde ein negativer Einfluss durch den Befall mit Larven von A. crassus auf das Milzgewicht beobachtet. Der Befall mit abgestorbenen Adulten war mit Anämie korreliert. Durch den kumulativen Effekt der Schwimmblasenschäden, die durch die Infektion mit A. crassus hervorgerufen werden, werden vor allen Dingen größere Aale im Bodensee-Obersee beeinträchtigt. Eine parasiteninduzierte Mortalität von A. crassus liegt jedoch nicht vor.

Aquatic vegetation and different substrates can be important factors for fish habitat. The European eel (Anguilla anguilla) is threatened by habitat loss in freshwater systems during its life stage as a yellow eel. To conserve the species, restoration measures and increased knowledge about its preference for benthic habitats are needed. In this study, I examined how macrophytes with varying densities affected the habitat selection of A. anguilla yellow eels during day and at night. A laboratory experiment was conducted in three stream flumes containing sand and stone habitats, with an open passage where the eels (n = 17) could swim between them. The stone habitat was the same in all flumes, whereas the sand habitat had three macrophytes densities: 14, 7 and 0 m-2. The number of times the eel switched between the habitats, the time to the first habitat switch and the proportion of time spent in each habitat was noted. The result showed no significant effect of vegetation density on the number of habitat switches, the time to the first switch, or the proportion of time the eels spent in each habitat. There was no difference between night and day regarding the number of switches between the habitats. However, the proportion of time spent in the habitats differed between night and day; the eels spent more time in the stone habitat at night than during the day (Z = 2,707, n = 17, p = 0,038). The results suggests that yellow eels are active at night and may leave their resting habitat among the vegetation to search for food when it gets dark. Aquatic vegetation may be an important feature of yellow eel habitat, and to restore these environments, targeted restoration efforts and an increased understanding of macrophyte occurrence and substrate composition are needed. / Akvatisk vegetation och olika bottensubstrat kan vara viktiga faktorer för fiskars habitat. Den Europeiska ålen (Anguilla anguilla) är i sitt livsstadium som gulål hotad av habitatförlust i sötvattensystem. För att bevara arten krävs restaureringsåtgärder och ökad kunskap om artens preferens av bentiska livsmiljöer. I denna studie undersökte jag hur makrofyter av olika täthet påverkade A. anguilla gulålars habitatval på dagen och natten. Ett laboratorieexperiment utfördes i tre strömrännor innehållandes ett sand- och ett stenhabitat, samt en öppen passage där ålarna (n = 17) kunde simma mellan dem. Stenhabitatet var densamma i alla strömrännor medan sandhabitatet hade tre tätheter av makrofyter; 14, 7, samt 0 m-2. Tid till första byte, antal gånger ålarna bytte mellan habitaten och proportionen av tiden som ålarna spenderade i de två habitaten noterades. Resultatet visade ingen signifikant effekt av växttäthet för tiden till första bytet, antalet byten eller proportionen tid som ålarna spenderade i vardera habitat. Det fanns ingen skillnad mellan natt och dag i avseende på antalet byten mellan habitaten. Däremot var proportionen tid som ålarna tillbringade i de två habitaten olika mellan dag och natt; ålarna var mer i stenhabitatet på natten än på dagen (Z = 2,707, n = 17, p = 0,038). Experimentet tyder på att gulålar är nattaktiva och kan lämna sitt vilohabitat bland växterna när det blir mörkt för att söka föda i stenhabitat. Akvatisk vegetation är en viktig del av ålars livsmiljöer och för att återställa dessa behövs riktade restaureringsinsatser och ökad förståelse för makrofyt- och substratpreferens.

Sedan 1970-talet har ålbeståndet minskat rejält och under de senaste decennierna har man försökt hitta en lösning på detta problem. En ökad naturlig rekrytering kan hjälpa ålarnas återhämtning. Varje år fastnar en stor mängd juvenila ålar vid vattenkraftverk. För att hjälpa ålarna ta sig förbi dessa kan man installera ramper, vilka hjälper ålarna att klättra förbi vattenkraftverken. Syftet med studien var att ta reda på vilken typ av substrat i ramper vid dessa vattenkraftverk som ålynglen föredrar. Studien utfördes utomhus i två odlingsbassänger där sex stycken ramper hade installerats. Den huvudsakliga studien bestod av ett selektionstest där ålynglen fick välja mellan tre olika substrat. Man antecknade också om det var några yngel som valde att stanna kvar, detta för att kunna testa om det fanns någon signifikant skillnad mellan importerade och vilda ålyngel när det gäller de yngel som inte valt någon av ramperna. Sammanlagt 500 yngel (vilda N=235, importerade N=265) var med i studien. Ålynglen placerades i nätkassar nedanför ramperna. Där hade de möjligheten att antingen välja ett av substraten och vandra uppför rampen, eller stanna kvar i bassängen. De tre substraten som testades var plastkätting, FishPass™ rigid brush mixed green (FishPass™) och Diadrain 25H (D-25). Resultatet blev att både de vilda och de importerade ålynglen föredrog FishPass™ och D-25 framför plastkätting. Det fanns ingen signifikant skillnad mellan FishPass™ och D-25. Man kom också fram till att signifikant fler vildfångade ålar än importerade valde att stanna kvar istället för att klättra upp för en ramp. Resultatet från den här studien säger att vi kan öka antalet juvenila ålar som tar sig förbi vattenkraftverk. Placerar man ut ramper klädda med antingen FishPass™ eller D-25 vid de vattenkraftverk där juvenila ålar har svårt att passera så kan man på så sätt hjälpa ålarnas naturliga rekrytering. / Since the 1970s, eel stocks have declined, and in the recent decades people have been trying to find a solution to this problem. An increased natural recruitment could help the eels to recover. Each year a large quantity of juvenile eels get stuck at hydroelectric plants. To help the eels pass by these plants, ramps can be installed, which help the eels to pass the dams. The purpose of the study was to find out what type of substrate in ramps at these hydropower plants that juvenile eels prefer. The study was conducted in two outdoor hatchery ponds where six ramps had been installed. The main study consisted of a selection test where juvenile eels had to choose between three different substrates. It was also recorded if there were some juveniles who chose to remain, in order to test if there was a significant difference between imported and wild eels when it comes to individuals that have not selected any of the ramps. A total of 500 juveniles (Wild N = 235, Imported N = 265) were included in the study. The juvenile eels were placed in net enclosures beneath the ramps, and had to choose one of the substrates and climb the ramp, or else remain in the net enclosure. The three substrates tested were plastic chain, Fish Pass ™ rigid brush mixed green (Fish Pass™) and Diadrain 25H (D-25). I found that both the wild and imported eels preferred Fish Pass™ and D-25 instead of the plastic chain. There was no significant difference between Fish Pass™ and D-25. It was also found that significantly more wild-caught than imported eels chose to stay in the enclosure instead of climbing up a ramp. The results from this study indicate that we can increase the number of juvenile eels that successfully pass by hydroelectric plants. If we place ramps covered with either Fish Pass™ or D-25 at the hydropower plants, we can help the eel’s natural recruitment.