31 |
Microfluidic Chemical Signal GenerationAzizi, Farouk 23 October 2009 (has links)
No description available.
|
32 |
Magnetic resonance microscopy of Aplysia neurons : studying neurotransmitter-modulated transport and response to stressJelescu, Ileana O. 02 October 2013 (has links) (PDF)
Recent progress in magnetic resonance imaging (MRI) has opened the way for micron-scale resolution, and thus for imaging biological cells. In this thesis work, we performed magnetic resonance microscopy (MRM) on the nervous system of Aplysia californica, a model particularly suited due to its simplicity and to its very large neuronal cell bodies, in the aim of studying cellular-scale processes with various MR contrasts. Experiments were performed on a 17.2 Tesla horizontal magnet, at resolutions down to 25 µm isotropic. Initial work consisted in conceiving and building radiofrequency microcoils adapted to the size of single neurons and ganglia. The first major part of the project consisted in using the manganese ion (Mn2+) as neural tract tracer in the buccal ganglia of Aplysia. Manganese is an MR contrast agent that enters neurons via voltage-gated calcium channels. We performed the mapping of axonal projections from motor neurons into the peripheral nerves of the buccal ganglia. We also confirmed the existence of active Mn2+ transport inside the neural network upon activation with the neurotransmitter dopamine. In the second major part of the project, we tested the potential of two diffusion MRI sequences for microscopy. On the one hand, we explored a very original mechanism for diffusion weighting, DESIRE (Diffusion Enhancement of SIgnal and REsolution), particularly suited for small samples. The two-dimensional DESIRE sequence was implemented and successfully tested on phantoms. The measured enhancement was consistent with theoretical predictions. Using this sequence to produce diffusion weighted images with an unprecedented contrast in biological tissue remains a challenge. On the other hand, a more "standard" sequence was implemented to measure the apparent diffusion coefficient (ADC) in nervous tissue with MRM. This sequence was a three-dimensional DP-FISP (Diffusion Prepared Fast Imaging with Steady-state free Precession), which met criteria for high resolution in a short acquisition time, with minimal artifacts. Using this sequence, we studied the changes in water ADC at different scales in the nervous system, triggered by cellular challenges. The challenges were hypotonic shock or exposure to ouabain. ADC measurements were performed on single isolated neuronal bodies and on ganglia tissue, before and after challenge. Both types of stress produced an ADC increase inside the cell and an ADC decrease at tissue level. The results favor the hypothesis that the increase in membrane surface area associated with cell swelling is responsible for the decrease of water ADC in tissue, typically measured in ischemia or other conditions associated with cell swelling.
|
33 |
Microtubule Assembly and Translocation Dynamics During Axonal ElongationKristi McElmurry (6636089) 25 June 2020 (has links)
<p> The urgency for deeper knowledge about nervous system function and dysfunction has never been greater. With increasing rates of mental disorders and expanding healthcare costs, deciphering details of axonal development is essential to meeting this imperative. Models of neuronal growth are improving as roles of microtubules and motor proteins surface. However, traditional motor protein studies focus on intracellular cargo transport, leaving deficits in knowledge about how these proteins organize cytoskeletal filaments in the axon and growth cone during neuronal development. Inconsistent findings on microtubule activity in growing axons also leave gaps in quantitative assessments of microtubule translocation and assembly, limiting the ability to construct a comprehensive model of axonal elongation.</p> The goal of this study was to provide a more complete neuronal growth cone model by determining how individual microtubule translocation and assembly, mass microtubule movements, and motor protein activity contribute to axonal elongation. The underlying mechanisms of these processes were investigated by testing the roles of dynein and microtubule dynamics in axonal elongation of <i>Aplysia </i><i>californica </i>neurons using transillumination, fluorescent speckle, and super-resolution microscopy. Pharmacologically inhibiting either dynein activity or microtubule assembly reduced both bulk and individual microtubule anterograde translocation and neurite elongation rates. Suppressing both processes simultaneously had compensatory rather than additive effects. Super-resolution imaging also revealed fewer dynein motors co-localized with microtubules when microtubule assembly was inhibited. These results strongly suggest that disrupting microtubule assembly blocks neurite outgrowth partly because it inhibits dynein-mediated bulk microtubule translocation.
|
34 |
Imagerie cellulaire par résonance magnétique rehaussée au manganèse (CelMEMRI) / Cellular manganese enhanced magnetic resonance imaging (CelMEMRI)Radecki, Guillaume 24 September 2015 (has links)
La science a avancé depuis le XIX ème siècle. Des nouveaux outils sont apparus : la microscopie optique nous donne la vision des cellules, la microscopie électronique nous entraine au cœur de celles-ci. L’imagerie par résonance magnétique est apparue dans les années soixante-dix. Depuis son évolution, l’IRM nous entraine de plus en plus loin dans les profondeurs secrètes de nos cerveaux. La possibilité d’observer l’activité neuronale à l’aide de l’imagerie fonctionnelle est une grande révolution. Cette thèse montrera la possibilité que l’on a d’observer l’activité d’un neurone individuel sans modification de son réseau grâce à l’imagerie rehaussée au manganèse. L’étude sera effectuée sur des Aplysies à très haut champ (17T). Ces animaux sont des mollusques marins gastropodes qui possèdent une particularité : leurs neurones sont de tailles importantes, ils peuvent atteindre 1 mm de diamètre. Leurs neurones sont regroupés en plusieurs ganglions. Mon étude portera sur le ganglion buccal, qui est le ganglion le plus étudié dans les recherches en électrophysiologie. Avant de réaliser les acquisitions, j’ai dû concevoir plusieurs antennes de tailles microscopiques adaptées à la taille des ganglions. En réduisant la taille des antennes, le rapport signal sur bruit augmente. Dans un deuxième temps, une double antenne a été développée permettant l’acquisition de deux échantillons simultanément. Cette antenne a nécessité de créer des préamplificateurs fonctionnant à 730 MHz. La première série d’expériences a permis d’observer l’évolution de l’activité neuronale selon différents stimulus liés au comportement alimentaire des aplysies in-vivo. J’ai montré grâce à la technique mise en place que l’on peut distinguer par IRM l’activité de chaque neurone face à un stimulus. Par la suite, pour continuer ce travail, une deuxième série d’expériences a été effectuée in-vitro. J’ai étudié le comportement des neurones selon les neuro-stimulateurs perfusés : la dopamine et la sérotonine, tous les deux présents naturellement dans l’aplysie. Globalement les neurones ont été activés mais après les avoir observés individuellement, j’ai remarqué quelques différences selon les neurotransmetteurs. Cette technique peut maintenant être utilisée pour étudier d’autres conduites de l’aplysie comme le comportement compulsif. L’étude sur la mémoire peut être aussi envisagée. Les origines comportementales ont probablement des mécanismes identiques entre les différentes espèces animales et donc avec l’Homme comme l’a démontré les études d’Eric Kandel sur la mémoire. / Science has evolved since the 19th century. New tools have appeared such as optical microscopy which gives us the vision of cells and electronic microscopy which leads us into their hearts. The magnetic resonance imaging appeared in the seventies. Evolving over time, the MRI has taken us farther and farther into the secret depths of our brains. The possibility of observing the neuronal activity thanks to the functional imaging is a major evolution. This thesis will show the possibility we have to observe the activity of a single neuron without modification of its network thanks to the manganese enhanced magnetic resonance imaging technique. The study was done on the Aplysia at very high field magnet (17T). These animals are marine gastropod mollusks with a peculiarity: their neurons are of important size and can reach 1 mm in diameter. Their neurons are grouped into several ganglia. My study concerns the buccal ganglion which is the most studied ganglia in the research in electrophysiology. Before making any acquisitions, I had to conceive several microscopic coils adapted to the size of the ganglions. By reducing the size of the coils, the signal of the noise ratio increases. Then, a double coil allowing the simultaneous acquisition of two samples was built. This antenna required the construction of pre-amplifiers operating at 730 MHz. The first series of experiments helped observe the evolution of the neuronal activity according to different stimuli linked to the eating habits of the Aplysia in vivo. Thanks to the technique implemented, I shall show that, using MRI, it is possible to distinguish the activity of each neuron with respect to a stimulus. Afterwards, to continue this work, a second series of experiments was made in vitro. I studied the behavior of neurons when perfused with neural stimulators: dopamine and serotonin, both naturally present in the Aplysia. Generally, all neurons were activated but when observing them individually, I noticed some differences. Studies in electrophysiology will allow us to get a better understanding and a confirmation of the results of this study. The MEMRI technique can be used in the future to study various disorders such as compulsive behaviors, which are present in the Aplysia, and probably have the same origins as in humans, given that many fundamental processes (such as memory studied by Eric Kandel who he demonstrated that human and Aplysia memories works with the same mechanism) are similar between the two species.
|
35 |
Magnetic resonance microscopy of Aplysia neurons : studying neurotransmitter-modulated transport and response to stress / Microscopie par résonance magnétique des neurones d’aplysie : étude du transport actif en présence de neurotransmetteurs, et de la réponse au stressJelescu, Ileana O. 02 October 2013 (has links)
Les progrès technologiques récents en imagerie par résonance magnétique (IRM) ont ouvert la voie à une résolution spatiale de l’ordre de quelques microns, et donc à l’imagerie de cellules biologiques. Dans le cadre de ce projet, nous avons réalisé des expériences de microscopie IRM sur le système nerveux de l’aplysie (Aplysia californica), particulièrement adapté de par sa simplicité et de par la très grande taille de ses neurones, en vue d’étudier des processus à échelle cellulaire avec divers contrastes IRM. Les expériences d’imagerie ont été effectuées sur un aimant horizontal 17.2 Tesla, à des résolutions spatiales jusqu’à 25 µm isotrope. Le travail initial a consisté en la conception et fabrication de micro-antennes radiofréquences adaptées à la taille de neurones uniques et de ganglions. La première partie du projet a porté sur l’utilisation de l’ion manganèse (Mn2+) comme traceur de réseaux neuronaux dans le ganglion buccal de l’aplysie. Le manganèse (Mn) est un agent de contraste IRM qui pénètre dans les neurones par les canaux de calcium. La cartographie des projections axonales des neurones moteurs du ganglion dans chacun des nerfs périphériques a été établie. Il a également été démontré l’existence d’un transport actif du Mn2+ au sein du réseau neuronal activé par le neurotransmetteur dopamine. Dans un second temps, on s’est intéressé à deux méthodes de mesure de diffusion par IRM, à échelle microscopique. D’une part, un mécanisme de pondération en diffusion, DESIRE (Diffusion Enhancement of SIgnal and REsolution), original et particulièrement adapté à des échantillons petits, a été exploré. La séquence DESIRE a été implémentée en deux dimensions et testée avec succès sur fantôme. Le rehaussement mesuré était en accord avec les prévisions théoriques. Le grand défi à venir sera d’utiliser cette séquence pour acquérir des images de tissu biologique pondérées en diffusion avec un contraste unique. D’autre part, une séquence plus « classique » a été implémentée pour mesurer le coefficient de diffusion apparent (ADC) dans le tissu nerveux. Il s’agit d’une DP-FISP (Diffusion Prepared Fast Imaging with Steady-state free Precession) en trois dimensions, qui répond aux critères de résolution spatiale et de rapidité, avec un minimum d’artefacts. Cette séquence a permis d’étudier l’évolution de l’ADC de l’eau à différentes échelles du tissu nerveux en réponse à un stress cellulaire. Les deux sollicitations retenues étaient un choc hypotonique ou l’ajout d’ouabaïne. Des mesures d’ADC ont été effectuées sur des corps neuronaux isolés et sur du tissu de ganglion, avant et après sollicitation. Les deux types de stress ont entraîné une augmentation de l’ADC dans la cellule et une diminution globale de l’ADC dans le tissu. Ces résultats soutiennent l’hypothèse que la diffusion ralentie de l’eau habituellement observée dans un tissu ischémié (ou dans d’autres conditions associées à un gonflement cellulaire) est due à l’augmentation de surface membranaire. / Recent progress in magnetic resonance imaging (MRI) has opened the way for micron-scale resolution, and thus for imaging biological cells. In this thesis work, we performed magnetic resonance microscopy (MRM) on the nervous system of Aplysia californica, a model particularly suited due to its simplicity and to its very large neuronal cell bodies, in the aim of studying cellular-scale processes with various MR contrasts. Experiments were performed on a 17.2 Tesla horizontal magnet, at resolutions down to 25 µm isotropic. Initial work consisted in conceiving and building radiofrequency microcoils adapted to the size of single neurons and ganglia. The first major part of the project consisted in using the manganese ion (Mn2+) as neural tract tracer in the buccal ganglia of Aplysia. Manganese is an MR contrast agent that enters neurons via voltage-gated calcium channels. We performed the mapping of axonal projections from motor neurons into the peripheral nerves of the buccal ganglia. We also confirmed the existence of active Mn2+ transport inside the neural network upon activation with the neurotransmitter dopamine. In the second major part of the project, we tested the potential of two diffusion MRI sequences for microscopy. On the one hand, we explored a very original mechanism for diffusion weighting, DESIRE (Diffusion Enhancement of SIgnal and REsolution), particularly suited for small samples. The two-dimensional DESIRE sequence was implemented and successfully tested on phantoms. The measured enhancement was consistent with theoretical predictions. Using this sequence to produce diffusion weighted images with an unprecedented contrast in biological tissue remains a challenge. On the other hand, a more “standard” sequence was implemented to measure the apparent diffusion coefficient (ADC) in nervous tissue with MRM. This sequence was a three-dimensional DP-FISP (Diffusion Prepared Fast Imaging with Steady-state free Precession), which met criteria for high resolution in a short acquisition time, with minimal artifacts. Using this sequence, we studied the changes in water ADC at different scales in the nervous system, triggered by cellular challenges. The challenges were hypotonic shock or exposure to ouabain. ADC measurements were performed on single isolated neuronal bodies and on ganglia tissue, before and after challenge. Both types of stress produced an ADC increase inside the cell and an ADC decrease at tissue level. The results favor the hypothesis that the increase in membrane surface area associated with cell swelling is responsible for the decrease of water ADC in tissue, typically measured in ischemia or other conditions associated with cell swelling.
|
36 |
The Role of Chemical Senses in Predation, Risk Assessment, and Social Behavior of Spiny LobstersShabani, Shkelzen 17 November 2008 (has links)
Chemical senses play a critical role in predator-prey and social interactions of many animals. Predators often evoke adaptive escape responses by prey, one of which is the release of chemicals that induce adaptive avoidance behaviors from both predators and conspecifics. I explore the use of chemicals in predator-prey and social interactions, using a crustacean model system, the spiny lobster. As predators, spiny lobsters are opportunistic, polyphagous feeders, and they rely heavily on their chemical senses during feeding. Some of their potential prey deter attacks through chemical defenses that act through the spiny lobsters’ chemical senses. An example of this is sea hares, Aplysia californica, which secrete an ink when vigorously attacked by sympatric spiny lobsters, Panulirus interruptus. I show that that this ink defends sea hares from spiny lobsters through several mechanisms that include phagomimicry, sensory disruption, and deterrence, and that the ink’s efficacy is enhanced by its naturally high acidity. As prey, spiny lobsters rely heavily on their chemical senses to assess risk from predators. One way to assess risk of predation is through ‘alarm cues’, which are injury-related chemicals. I show that injured Caribbean spiny lobsters, Panulirus argus, release alarm cues in their hemolymph, and that nearby conspecifics detect these cues using olfaction. Hemolymph from conspecifics induces primarily alarm behavior in the form of retreat, sheltering, and suppression of appetitive responses. In contrast, hemolymph from heterospecifics, depending on phylogenetic relatedness, induces either mixed alarm and appetitive behaviors or primarily appetitive behaviors. Spiny lobsters also use chemical cues to assess risk during social interactions with conspecific. I show that spiny lobsters use urine-borne chemical signals and agonistic behaviors to communicate social status and that these chemical signals are detected exclusively by the olfactory pathway. Dominant animals increase urine release during social interactions, whereas subordinates do not. Experimental prevention of urine release during interactions causes an increase in agonism, but this increase is abolished when urine of dominants is reintroduced. My findings lay the foundation for neuroethological studies of risk-assessment systems mediated by intraspecific chemical cues.
|
37 |
Neural Correlates of Adaptive Responses to Changing Load in Feeding <i>Aplysia</i>Gill, Jeffrey Paul 29 May 2020 (has links)
No description available.
|
38 |
<b>Post-translational modifications governing neuro-migration and infection</b>Sherlene Brown (18087418) 04 March 2024 (has links)
<p dir="ltr">This dissertation delves into two research projects that aim to characterize post-translational modifications in two distinct proteins, each originating from a different species – one from the eukaryotic sea slug Aplysia californica and the other from the bacterial pathogen Bordetella bronchiseptica.</p><p dir="ltr">Aplysia have an unusually large neuron and therefore serve as an excellent model for studying cell signaling regulating neuronal chemotaxis. Cortactin is an actin binding protein that is regulated by post-translational modifications, including acetylation and phosphorylation. Studies have shown that Src2 tyrosine kinase phosphorylates cortactin to regulate lamellipodia protrusion and filopodia formation in Aplysia bag cell neurons. However, these in vivo phenotypes have not been tested mechanistically in vitro. To this end, the goal of my thesis work was to validate in vivo observations. The following work describes the methodology we developed to purify homogenous non-phosphorylated proteins. Our collaborative results show that Src2 phosphorylates cortactin at Y499, although Y505 is the preferred site in vitro.</p><p dir="ltr"> Filamentation induced by cAMP (Fic) proteins constitute a recently characterized family of enzymes that are being recognized to regulate diverse cellular processes in bacteria and metazoans. While Fic proteins predominantly utilize adenosine triphosphate (ATP) to post-translationally modify target proteins via a covalent addition of AMP, two Fic proteins have been reported that utilize uridine triphosphate (UTP) and cytidine diphosphate-choline (CDP-choline) to alter the activity of their target. In this dissertation, we report the discovery of the first guanosine triphosphate (GTP) specific Fic protein – BB0907 (BbFic) from Bordetella bronchiseptica. BbFic displays weak to no binding to ATP; instead has a 10-fold increased preferential usage for GTP. We identify key residues involved in GTP recognition. Additionally, sequence similarity network (SSN) analyses reveal that BbFic represents a distinct clade of Fic proteins, highlighting BbFic as a representative new class of guanylyltransferase. Our discovery adds to the functional diversity of the growing Fic protein family and frames the groundwork for understanding Fic-mediated GMPylation as a novel signaling paradigm. </p><p dir="ltr">Taken together, my thesis work provides novel insights into biological consequences of Fic-mediated GMPylation in bacteria and Src-mediated phosphorylation in filopodia formation.</p><p><br></p>
|
39 |
Distinguishing Behavior from Highly Variable Neural Recordings Using Machine LearningSasse, Jonathan Patrick 04 June 2018 (has links)
No description available.
|
Page generated in 0.0372 seconds