O presente trabalho apresenta uma discussão de metodologia para o diagnóstico e ações para redução de perdas de água, auxiliando na determinação do perfil das perdas, permitindo uma melhor priorização das ações de combate e redução de perdas reais (físicas) e perdas aparentes (comerciais). Para o desenvolvimento desta metodologia, foi realizada uma ampla revisão bibliográfica, onde é apresentada a conceituação geral sobre perdas de água, suas causas e ocorrências, indicadores, ações para redução, e uma abordagem detalhada quanto às metodologias existentes para avaliação de perdas e ferramentas disponíveis para isto. A metodologia aqui aplicada desenvolve a avaliação das perdas através do balanço hídrico, utilizando o software gratuito desenvolvido pelo Banco Mundial (W-B Easy Calc - v1.17), e foi aplicada no sistema de abastecimento de água da Região Metropolitana de São Paulo (RMSP). Foram levantados os dados básicos de controle do sistema e realizados ensaios de campo, com medições de pressão e ensaios para determinação das perdas aparentes. Como estudo de caso, a metodologia foi aplicada em três setores de abastecimento da área central da RMSP, com características distintas. Os resultados do diagnóstico das perdas foram coerentes com as expectativas. Em função dos resultados, em cada setor deverá ser desenvolvida uma estratégia específica para a redução de perdas. / This study presents a discussion of a methodology for diagnosis and actions to reduce water losses, to assist in determining the profile of water losses, allowing a better prioritization of actions to combat and reduce real (physical) losses and apparent (commercial) losses. For developing the methodology, it was conducted an extensive bibliography review, where is presented the general concepts on water losses, its causes and events, indicators, actions to reduce, and a detailed discussion about the existing methodologies to assess losses and tools available for this. The applied methodology consists of an assessment of losses through the water balance, using free software developed by the World Bank (WB Easy Calc - v1.17) and it was applied in the water supply system in metropolitan region of Sao Paulo. To do this, it was necessary to collect basic data of system control and field tests, with pressure measurements and tests to determine apparent losses. As case study, the methodology was applied in three supply zones at the central area of metropolitan region of Sao Paulo, with different characteristics. The results of water losses diagnosis were consistent with expectations. With these results, a specific strategy should be developed in each sector for reducing losses.

O presente trabalho visa analisar as implicações e desafios impostos ao direito penal na tutela da criminalidade econômica atual, especialmente no que tange ao delito de gestão fraudulenta de instituição financeira, previsto no caput do artigo 4º da Lei 7.492/86, delito mais severamente apenado na Lei dos Crimes contra o Sistema Financeiro Nacional. Em razão de sua descrição absolutamente genérica e da gravidade da sanção cominada buscar-se-á interpretar suas elementares típicas de forma conectada aos motivos que ensejaram a sua edição, bem como relacionada ao bem jurídico tutelado pela norma, aplicando-se redutores teleológicos no desiderato de conferir ao tipo uma identidade própria, agregando coerência interna na própria lei e minimizando os recorrentes problemas quanto ao âmbito de incidência de seus dispositivos legais, por vezes dispostos em situação de conflito aparente de normas. Nesses termos, o crime de gestão fraudulenta de instituição financeira será decomposto em todos os seus elementos típicos, objetivos e subjetivos, observando-se sua objetividade jurídica, objeto material, sujeitos ativos, passivos, concurso de pessoas, consumação e tentativa. Posteriormente, será adentrado à problemática do concurso aparente de normas entre o crime estudado gestão fraudulenta de instituição financeira e os demais tipos penais previstos na Lei 7.492/86, especialmente em relação aos tipos penais previstos nos artigos 5º, 6º, 9º, 10, 11, 16, 17, 21 e 22. A análise será feita com base nas relações lógico-conceituais entre os preceitos normativos, seguida de uma interpretação teleológica e valorativa, com base nos critérios de resolução de conflito aparente de normas propostos pela doutrina especialidade, subsidiariedade, consunção e alternatividade. Ao final, as conclusões encontradas serão confrontadas com o recorte jurisprudencial dos julgados atinentes à matéria, proferidos pelo Tribunal Regional Federal da 3º Região nos últimos 10 anos (01/01/2003 a 31/12/2013). / This work intends to analyze the implications and challenges imposed on criminal law for the defense of current economic crimes, especially in regards to the crime of mismanagement of financial institutions provided for in the main provision of Article 4 of Law No. 7492/86, a crime punished by maximum sentence in the Law of Crimes against the National Financial System. As a result of its completely general description and the severity of the sanction imposed, the interpretation of its typical elements shall be made in connection with the motives which originated the enactment thereof, as well as relating to the legal interest protected by the rule, while applying teleological reducers for the purpose of conferring a proper identity to the definition of the crime, adding internal consistency to the law itself and minimizing recurring problems regarding the scope of incidence of the legal provisions thereof, at times applied in situations of apparent conflict of rules. This way, the crime of mismanagement of financial institutions will be decomposed into all its typical objective and subjective elements, addressing legal objectivity, material object, perpetrators, victims, co-perpetration, consummation and attempt. Next, it will address the issue of the apparent joinder of rules between the crime examined - mismanagement of financial institution - and other criminal offenses established by Law 7492/86, especially in relation to criminal offenses provided for in Articles 5, 6, 9, 10, 11, 16, 17, 21 and 22. The analysis will be based on logical-conceptual relations between the normative precepts, followed by a teleological and judgmental interpretation, based on the solution criteria of apparent conflict of rules proposed by the jurists - specialty, subsidiarity, merger and alternativity. Finally, the conclusions reached will be confronted with case law clippings of decisions regarding the matter granted by the Federal Regional Court of the third Region in the past 10 years (01/01/2003 to 12/31/2013).

O objetivo deste estudo foi avaliar diferentes concentrações de monensina sódica na ração de vacas em lactação e seus efeitos sobre o consumo e digestibilidade aparente total da matéria seca e dos nutrientes, produção e composição do leite, fermentação ruminal, síntese de proteína microbiana, parâmetros sanguíneos e resíduos de monensina no leite. Foram utilizadas 12 vacas da raça Holandesa, distribuídas em três quadrados latinos 4x4 balanceados, alimentadas com as seguintes rações: 1) Controle (C), composto por ração basal sem adição de monensina, 2) Monensina 12 (M12), adição de 12 mg/kg MS de monensina na ração, adicionada ao concentrado, 3) Monensina 24 (M24), adição de 24 mg/kg MS de monensina na ração, adicionada ao concentrado e 4) Monensina 48 (M48), adição de 48 mg/kg MS de monensina, adicionada ao concentrado. A produção de leite e o consumo de matéria seca foram mensurados diariamente durante todo o período experimental. As amostras utilizadas para análise da composição do leite foram coletadas no 16o dia de cada período experimental, sendo provenientes das duas ordenhas diárias. As amostras de sangue foram coletadas em tubos vacuolizados por punção da veia e/ou artéria coccígea. As amostras de líquido ruminal foram coletadas com a utilização de sonda esofágica três horas após a alimentação matinal. A digestibilidade foi determinada por meio de indicador interno FDAi. Houve efeito linear decrescente do consumo de matéria seca, matéria orgânica, proteína bruta, extrato etéreo, carboidratos totais, fibra detergente neutro, carboidratos não fibrosos e nutrientes digestíveis totais. Não houve diferença na digestibilidade aparente total da matéria seca e dos nutrientes em função das rações experimentais, excetuando-se a digestibilidade aparente total da proteína bruta e fibra detergente neutro, que foram observados efeitos linear crescente e decrescente, respectivamente. Não houve efeito das rações experimentais sobre os valores de pH ruminal. A suplementação com monensina sódica nas dietas resultou em alteração da proporção molar de propionato no líquido ruminal, além de diminuir a relação acetato:propionato. As rações contendo monensina sódica apresentaram maior proporção molar de propionato e menor de acetato em relação à ração controle, e, de forma semelhante, apresentaram menor relação acetato:propionato. Não houve efeito das dietas experimentais nas excreções diárias totais de urina, de alantoína e de acido úrico na urina, e de derivados de purinas totais. Não houve diferença na porcentagem de alantoína nos derivados de purina, das purinas absorvidas e do nitrogênio microbiano para as rações experimentais. Foi observado aumento de produção de leite para as vacas alimentadas com as concentrações intermediárias de monensina sódica nas rações. Não houve efeito da adição de monensina sódica nas rações sobre os parâmetros sangüíneos glicose, colesterol total, proteína total, albumina, aspartato aminotransferase e γ-glutamiltransferase. Foi observado efeito linear crescente com a adição de monensina sódica nas rações sobre a uréia e nitrogênio uréico do soro. Os resíduos de monensina sódica detectados no leite estão dentro do limite máximo estabelecido pela FAO/WHO. A utilização de monensina sódica nas rações de vacas leiteiras no terço médio de lactação influencia o desempenho produtivo dependendo da dose utilizada, e não resulta em resíduos no leite independentemente da dose utilizada. / This aim this study was evaluate the use of different concentrations of sodic monensin the feed of dairy cows and its effects on consumption and total apparent digestibility of dry matter and nutrients, milk yield and milk composition, ruminal fermentation, synthesis microbial protein, blood parameters and sodic monensis residues in milk. Twelve Holstein cows were allocated in three balanced Latin squares 4x4, and fed with the following rations: 1) control (C), basal diet without sodic monensin addition, 2) monensin 24 (M24), addition of 24 mg/kg DM of monensin in the ration, added to concentrate, and 3) monensin 48 (M48), addition of 48 mg/kg DM of monensin in the ration, added to concentrate. Milk yield and dry matter intake were measured daily throughout the experimental period. The samples used for analysis of milk composition were collected on the 16th day of each period, and from the two milkings. Blood samples were collected in vacuolated tubes by puncture or coccygeal vein or artery. Samples of rumen fluid were collected with use of esophageal probe three hours after the morning feeding. The digestibility was determined by means of an internal indicator iADF. There was linear effect dry matter intake, organic matter, crude protein, ether extract, total carbohydrates, neutral detergent fiber, non-fiber carbohydrates and total digestible nutrients. There was no difference on apparent total tract digestibility of dry matter, organic matter, ether extract, total carbohydrates, non-fiber carbohydrates and total digestible nutrient values observed for the experimental diets. There was no difference in total apparent digestibility of dry matter and nutrients depending on the experimental diets, except for the total apparent digestibility of crude protein and neutral detergent fiber, wich effects were observed linear ascending and descending, respectively. There was no effect for ruminal pH values. Supplementation with sodic monensin in the diets resulted inchanging the molar proportion of propionate in ruminal fluid while decreasing the acetate:propionate. The rations containing monensin had higher molar ratio of acetate propionate and smaller relation to the control diet and similary had a lower acetate:propionate ratio. There was no effect in total daily urine excretion of allantoin and uric acidin urine and total purine derivates. There was no difference in the percentage of allantoin in the purine derivates, absorbed purines and microbial nitrogen to the experimental diets. We observed increase in milk yield for cows fed the intermediate concentration of monensin in the feed. There was no effect of monensin in feed on the blood parameters glucose, total cholesterol, total protein, albumin, aspartate aminotransferase and γ-glutamyltransferase. Increased linearly, with the addition of monensin in the feed on the urea and serum urea nitrogen. The detected residues of sodic monensin in milk was smaller that the maximum limit established for FAO/WHO. The use of monensin in diets cows in the middle third of lactation influences growth performance depending on the dose used, and does not result in residues in milk regardless of dose.

Marginally outer-trapped surfaces (MOTSs) are found for a family of space-like hypersurfaces described by the Brill-Lindquist initial data. These hypersurfaces contain a singular ring characterized by its radius, mass and charge. Due to the ring character of the singularity, these surfaces are natural candidates for MOTSs with toroidal topology. By adjusting and employing the numerical method of geodesics, we indeed localize MOTSs of both spherical and toroidal topology, and compare the results with those obtained previously by Jaramillo & Lousto.

La perméabilité est l'un de paramètres importants pour tous les procédés faisant intervenir des transferts couplés de chaleur et de masse. Sa valeur est directement liée à la morphologie du réseau de pores, clairement double échelle dans le cas du bois. Il existe plusieurs outils d’investigation 3-D par exemple la micro-tomographie voire nano-tomographie pour décrire la morphologie des pores. Néanmoins, ces investigations 3-D échouent pour les plus petits pores dans le bois, qui peuvent avoir une taille largement inférieure au micromètre. Ce travail de thèse utilise l'effet du libre parcours moyen du gaz sur la perméabilité apparente pour identifier la taille des pores utilisés par le cheminement du fluide.Une approche équilibrée entre expérimentation et modélisation est proposée. Dans la première partie du travail, nous avons développé un dispositif original destiné à mesurer la perméabilité apparente sur une large plage de niveau de pression moyenne. Ce dispositif ne comporte pas de débitmètre : le flux massique est simplement obtenu par la relaxation de la différence de pression entre deux réservoirs de volume connu.Ce dispositif a permis de mesurer la perméabilité apparente en fonction de la pression moyenne pour différents matériaux. Les valeurs obtenues de perméabilité intrinsèque sont en bon accord avec les publications précédentes.L'effet de la pression moyenne sur la perméabilité apparente a été analysé pour identifier des paramètres structuraux des milieux poreux. Partant d'un agencement série/parallèle de pores, les paramètres structuraux sont identifiés par méthode inverse en tirant profit du changement de régime d’écoulement avec le nombre de Knudsen (du régime de Darcy vers le régime de glissement moléculaire lorsque le nombre de Knudsen passe de zéro à l’infini).Cette approche a été validée avec des membranes à pore monodisperse grâce à une observation en microscopie électronique à balayage. Les paramètres ont ensuite été identifiés sur des échantillons de bois, selon différentes directions et sur des matériaux à base de bois. Ils confirment certaines données de la littérature, et apportent un éclairage nouveau, notamment sur les chemins de migration transverse chez le hêtre. / Permeability is one of the important parameters for all processes involving coupled heat and mass transfer. Its value is directly related to the morphology of the pore network, clearly a dual scale organisation in the case of wood. Nowadays, several 3D investigative tools exist, such as micro-tomography or nano-tomography. However, these 3-D investigations fail for the smallest pores active in fluid flow in wood, due to their submicron size. The present work takes advantage of the effect of the mean free path on the apparent gaseous permeability to identify the pore size of the pore network.A balanced approach between experimentation and modelling is proposed. In the first part of the work we have developed an original device for measuring the apparent permeability over a wide range of average pressure. This device was conceived to work without flowmeter: the mass flow is obtained by the relaxation of the pressure difference between two tanks. This device was used to measure the apparent permeability as a function of the average pressure for different materials.The intrinsic permeability values obtained are in good agreement with literature data.The effect of the average pressure on the apparent permeability was analysed to identify structural parameters of the porous media. Starting from a serial / parallel arrangement, the structural parameters are identified by inverse method taking advantage of the dependence of the flow regime with Knudsen's number (from Darcy's regime to pure molecular slip regime when Knudsen's number shifts from zero to infinity).This approach was validated with a monodisperse membrane, whose pore size was determined using a scanning electron microscopy. Structural parameters were then identified on wood samples, measured along different directions and on wood-based materials. They confirm literature data and bring new outcomes, namely regarding the fluid pathway in beech in radial and tangential directions.

Desde 1814, quando então se deram seus primeiros estudos, a mecânica de sistemas de massa variável tem se constituído como um ramo particular dentro da mecânica clássica. Suas aplicações encontram-se espalhadas por diversas áreas do conhecimento e vão desde a engenharia até a medicina, por exemplo. No entanto, apesar dessas aplicações de sucesso, ainda hoje são encontradas na literatura discussões acerca dos fundamentos da mecânica de sistemas de massa variável. Nesse cenário, figuram os chamados aparentes paradoxos que envolvem diferentes equações de movimento para um mesmo sistema de massa variável. É o que pode ser encontrado, por exemplo, com relação ao problema de Wagner, no âmbito do estudo do impacto de corpos sólidos contra superfícies de líquidos, e ao problema da corrente em queda. Nessa tese, questões como essas serão abordadas. Mas o cerne do escopo do presente trabalho é a apresentação de uma discussão de caráter mais geral e interpretativa sobre a teoria e aplicação da mecânica de sistemas de massa variável, mantendo-se como foco principal a contribuição para um melhor entendimento desse importante ramo da mecânica. Para tal, resultados teóricos originais serão apresentados, e discussões e aplicações sobre os mesmos serão feitas. Inicialmente, uma discussão sobre os primeiros trabalhos que fundamentam a dinâmica de uma partícula de massa variável é feita. Nesse contexto, interpretações originais do autor dessa tese são apontadas. Em seguida, a aplicação da equação de Lagrange a sistemas de massa variável é abordada. Nesse cenário, esse autor apresenta a chamada equação de Lagrange para um volume de controle onde a massa varia com as coordenadas e velocidades generalizadas. Esse também é um dos resultados originais dessa tese. Por fim, é apresentada a extensão do princípio variacional para um líquido para um volume de controle, que também é um resultado original desse trabalho. Dois problemas clássicos dentro da teoria de sistemas de massa variável são então tratados, i.e. o problema da corrente em queda e o problema de Wagner. Trata-se de dois problemas aparentemente paradoxais. A resolução desses aparentes paradoxos é abordada, o que também se constitui em um dos resultados originais dessa tese. Uma breve discussão sobre o problema do colapso das torres gêmeas do World Trade Center à luz da mecânica de sistemas de massa variável é também feita. / Since 1814, when the first researches on the topic were carried out, variable mass system mechanics has become a particular branch within classical mechanics. Applied problems involving variable mass systems are sparsely distributed over a wide range of different areas of knowledge, and go from engineering to medicine, for example. However, despite these successful applications, even today one can find in the specialized literature discussions on the fundamentals of the variable mass system mechanics. In this scenario, apparent paradoxes, which are based on different equations of motion for a same variable mass system, figure out. In this sense, the Wagners problem, in the context of the study of the impact of solid bodies into liquid surfaces, and the falling chain problem can be cited as didactic examples. In this thesis, topics like this one will be treated. However, the main scope of this work is to present a more general and interpretative discussion on both the theory and application of the mechanics of variable mass systems, but keeping the focus on contributions that enable a better understanding of such an important branch of mechanics. For that, original theoretical results will be presented, as also discussions and applications of them. In the beginning, a discussion on the first fundamental works about the dynamics of a variable mass particle is done. In such a context, original interpretations of this author are pointed out. Then, the application of Lagrange equations on variable mass systems is discussed. In this scenario, this author shows the so-called Lagrange equation for a control volume where mass varies with generalized coordinates and velocities. This is also an original result of this thesis. By the end, an extension of a variational principle to a control volume is shown, also an original result of this work. Two classical problems within the theory of variable mass systems are then treated, i.e. the falling chain problem and the Wagners problems. Both are apparently paradoxical problems. The resolution of such apparent paradoxes is addressed, what is also an original result. Within the present context of the mechanics of variable mass systems, a brief discussion on the problem of the collapse of the World Trade Center twin towers is also done.

Hypertension is the chronic elevation in blood pressure that is regulated in part through the retention and regulation of sodium retention and excretion in the kidneys. Hence the kidney has been considered the organ that regulates blood pressure. There are a cohort of patients that suffer with high blood pressure due to lack of 11β-hydroxysteroid dehydrogenase-type 2 (11β-HSD2) expression (which inactivates glucocorticoids (GCs), allowing selective activation of mineralocorticoid receptors (MR) by aldosterone) that results in hypertensive and increased salt appetite phenotypes - a condition known as syndrome of apparent mineralocorticoid excess (SAME). This disorder can be recapitulated in the mouse through the global deletion of 11β-HSD2, which results in over activation of the MR driving an elevation in blood pressure. However, the distinction between blood pressure elevation because of kidney dysfunction with loss of 11β-HSD2 or increased salt appetite due to loss of brain 11β-HSD2 expression is not clear from the global 11β-HSD2 knockout model. Salt appetite is regulated by regions of the brain out-with the blood-brain barrier, known as circumventricular organs. In the mouse, salt appetite is controlled by aldosterone-sensitive cells in the nucleus of the solitary tract (NTS) in the brain stem, where 11β-HSD2 is expressed to provide mineralocorticoid selectivity. However, in the fetal brain, 11β-HSD2 is widely expressed, protecting against adverse GC action that alters brain development and increases susceptibility to psychiatric disorders as adults. 11β-HSD2 deletion solely in the brain from embryonic day 12 resulting in GC fetal programming (HSD2BKO) causes effects on both behaviour and salt appetite. To determine the role of developmental versus adult expression of brain 11β- HSD2, mice with deletion of brain 11β-HSD2 from mid gestation (HSD2BKO) and mice with adult deletion of 11β-HSD2 in the NTS using lentivirus (HSD2.v- BKD) were compared. The phenotypes (salt appetite, blood pressure (BP), baroreceptor response (BRR) and cognition), can be categorised as either due to GC fetal programming (as indicated by HSD2BKO groups), or increased activation of MR in adult 11β-HSD2 expressing neurons (recapitulated in the HSD2.v-Cre groups). Salt appetite increased in both HSD2BKO and HSD2.v-BKD cohorts (mean percentage increase 65% n=8 and 46% n=6, compared to their respective controls), leading to an increased BP in both groups (+12% and +8%, respectively) as well as an impaired BRR, indicating all phenotypes are mediated by adult NTS neurons. However, spatial recognition memory (Object-in-Place task) is abolished in HSD2BKO mice, whereas, HSD2.v-BKD mice still retain short-term memory. Our data suggest that neural 11β-HSD2 protects against inappropriate activation of MR by corticosterone to regulate salt appetite and salt-induced rises in blood pressure. However, spatial recognition memory is not influenced by deletion of 11β-HSD2 in the adult brain, confirmation that this phenotype is underpinned by developmental programming by GCs, which is observed in the 11β-HSD2 brain KO. Salt appetite has been shown to be centrally regulated through the adult deletion of 11β-HSD2. From this, our data suggest that an increased salt appetite is due to adult loss of function of 11β-HSD2 rather than GC programming during development. Highlighting the NTS as a region for drug delivery to try and control salt appetite in salt sensitive individuals who struggle with administering a recommended change in diet. To develop this further, minimally invasive modes of delivery of viruses and drugs into the brain were investigated. In so doing, a non-invasive and reversible method to temporarily disrupt the blood brain barrier (BBB) was optimised. The technique required acoustic insonation of ultrasonic contrast agents (CAs) (gas microbubbles) adjacent to the BBB. These microbubbles (SonoVueTM, Bracco) were delivered via tail vein injection into the vasculature. To target the BBB, an ultrasonic transducer was suspended and focused through coupling gel onto the area of interest in the brain with skull the intact. The optimisation of this technique required determination of the focal position of the 3.5MHz transducer that was utilised, in addition to optimisation of the pulse length, pulse repetition frequency and power output of the ultrasound beam to enable the BBB to be disrupted. In addition, measurement of the attenuation of the ultrasound beam through ex vivo mouse skulls were measured. These results showed a 50% reduction in pressure amplitude from the baseline of 335.2mV (Baseline mean = 100% +/-SEM 0 n=3 (No skull), five regions across the skull averaged 47.79% +/-SEM 1.913 n=25 (using 5 different animals). In in vivo mice, after co-injection of the microbubbles with Evans Blue and insonation of the brain, disruption of the BBB was confirmed by the presence of Evans Blue dye in the brain, with no measurable damage occurring in the brain. This was confirmed by cell and nuclear morphology with no red blood cell extravasation into the surrounding tissue. The parameters used to open the BBB used a peak negative pressure of 2.1MPa (single pulse), transducer frequency 3.5MHz, 35,000 cycles over a 10ms burst at a pulse repetition frequency of 10Hz. The technique when applied in vivo in recovery animals is speculated to work by the focused ultrasound causing the microbubbles to oscillate within the vasculature adjacent to the BBB, resulting in high-shear stresses being generated on the tight junctions within the BBB. The resultant gaps in the BBB allow free circulating compounds (e.g. large dye molecules (Evans Blue - 960.8g/mol molecular weight) and adeno-associated-viruses (25nm with a packing capacity of 4.5kb) within the blood to pass into the brain, but there is no penetration of red blood cells (7μm). Longitudinal mouse experiments demonstrated that within 12-hours these gaps close with no long-term damage observed. Currently, utilising this technique, successful passage of an adeno-associated virus expressing GFP (as a marker) has been shown to pass into the brain (n=6 for each cohort including control) - indicating that the virus requires the ultrasound and microbubbles to facilitate its movement into the brain. Further technique optimisation is being explored looking at the role of CAs used in the opening and disruption of the BBB, comparing composition and size of the CAs. Microbubbles (2-3μm) and nanobubbles (200nm) were compared as well as lipid and non-ionic surfactant surface compositions, using volume of drug delivery and degree of disruption as outputs. Using this technique, the hydrophilic drug mimic calcein was delivered into the brain (n=5 non-ionic surfactant nanobubble, n=5 lipid nanobubble). Results have indicated that the delivery of calcein is most efficient when using non-ionic surfactant nanobubbles as opposed to lipid nanobubbles - with a greater volume of the drug being delivered into the cerebral tissue. Furthermore, the concentration and surface composition of the nanobubble have an effect as to the size and potential damage to the brain when opening the BBB. In conclusion, it has been shown that it is possible to non-invasively open the BBB and deliver viruses and dye into the brain. In addition, this thesis has investigated the use of nanobubbles as both facilitators to opening the BBB and delivery vectors for potentially therapeutic drugs. Finally, a non-invasive opening of the BBB has been achieved using focused ultrasound. Ultimately this non-invasive opening of the BBB can be used to achieve delivery of larger molecules (such as antibodies and viruses) into the brain to target treatments. Focused ultrasound brain targeting can be applied to the potential treatment of salt appetite regulation in the NTS. For the individuals who suffer from salt sensitive hypertension, the NTS can be targeted to reduce the drive to ingest high salt diets. Furthermore, the continuation of research into the central control of BP, salt appetite and baroreceptor reflex control can become better understood, using less invasive delivery techniques to the brain.

Made available in DSpace on 2014-12-17T15:42:27Z (GMT). No. of bitstreams: 1 AlineAAO_TESE.pdf: 3075922 bytes, checksum: bd3bdc6aafacdcbe05784c0fbe751097 (MD5) Previous issue date: 2013-08-09 / The mesoporous nanostructured materials have been studied for application in the oil industry, in particular Al-MCM-41, due to the surface area around 800 to 1.000 m2 g-1 and, pore diameters ranging from 2 to 10 nm, suitable for catalysis to large molecules such as heavy oil. The MCM-41 has been synthesized by hydrothermal method, on which aluminum was added, in the ratio Si/Al equal to 50, to increase the generation of active acid sites in the nanotubes. The catalyst was characterized by X-ray diffraction (XRD), surface area by the BET method and, the average pore volume BJH method using the N2 adsorption, absorption spectroscopy in the infrared Fourier Transform (FT-IR) and determination of surface acidity with application of a probe molecule - n-butylamine. The catalyst showed well-defined structural properties and consistent with the literature. The overall objective was to test the Al-MCM-41 as catalyst and thermogravimetric perform tests, using two samples of heavy oil with API ? equal to 14.0 and 18.5. Assays were performed using a temperature range of 30-900 ? C and heating ratios (β) ranging from 5, 10 and 20 ?C min-1.The aim was to verify the thermogravimetric profiles of these oils when subjected to the action of the catalyst Al- MCM-41. Therefore, the percentage ranged catalyst applied 1, 3, 5, 10 and 20 wt%, and from the TG data were applied two different kinetic models: Ozawa-Flynn-Wall (OFW) and Kissinger-Akahrira-Sunose (KAS).The apparent activation energies found for both models had similar values and were lower for the second event of mass loss known as cracking zone, indicating a more effective performance of Al-MCM-41 in that area. Furthermore, there was a more pronounced reduction in the value of activation energy for between 10 and 20% by weight of the oil-catalyst mixture. It was concluded that the Al-MCM-41 catalyst has applicability in heavy oils to reduce the apparent activation energy of a catalyst-oil system, and the best result with 20% by weight of Al-MCM-41 / Os materiais mesoporosos nanoestruturados vem sendo estudados para aplica??o na ind?stria do petr?leo, em especial o Al-MCM-41, devido ? ?rea superficial em torno de 800-1000 m2g-1 e di?metro de poros variando de 2 a 10 nm, adequado para cat?lise de mol?culas grandes como petr?leos pesados. O MCM-41 foi sintetizado pelo m?todo hidrot?rmico, o qual foi adicionado Alum?nio numa propor??o Si/Al igual ? 50, a fim de aumentar a gera??o de s?tios ?cidos ativos nos nanotubos. O catalisador foi caracterizado por difra??o de Raios-X (DRX), ?rea superficial pelo m?todo BET e volume m?dio de poros pelo m?todo BJH atrav?s da adsor??o de N2, espectroscopia de absor??o na regi?o do infravermelho por Transformada de Fourier (FT-IR) e determina??o da acidez superficial com aplica??o de uma mol?cula sonda nbutilamina. O catalisador apresentou propriedades estruturais bem definidas e coerentes com a literatura. O objetivo geral foi testar o Al-MCM-41 como catalisador e realizar ensaios termogravim?tricos, utilizando duas amostras de petr?leo pesado com ?API iguais ? 14,0 e 18,5. Os ensaios foram realizados usando uma faixa de temperatura de 30 900?C, e raz?es de aquecimento (β) variando de 5, 10 e 20 ?Cmin-1. O intuito era verificar os perfis termogravim?tricos destes petr?leos quando submetidos ? a??o do catalisador Al-MCM-41. Para tanto, variou-se o percentual de catalisador aplicado in situ: 1, 3, 5, 10 e 20% em massa e, a partir dos dados termogravim?tricos foram aplicados dois modelos cin?ticos diferentes: Ozawa-Flynn-Wall (OFW) e Kissinger- Akahrira-Sunose (KAS). As energias de ativa??o aparente encontradas para ambos os modelos tinham valores semelhantes e eram menores para o segundo evento de perda de massa conhecido como zona de craqueamento, indicando uma atua??o mais efetiva do Al-MCM-41 nessa zona. Al?m disso, constatou-se uma diminui??o mais acentuada no valor das energias de ativa??o para valores entre 10 e 20% em massa da mistura catalisador-?leo. Concluiu-se que o Al-MCM-41 possui aplicabilidade catal?tica em petr?leos pesados diminuindo a energia de ativa??o aparente de um sistema catalisador?leo, sendo o melhor resultado com 20% em massa de Al-MCM-41

O presente trabalho apresenta uma discussão de metodologia para o diagnóstico e ações para redução de perdas de água, auxiliando na determinação do perfil das perdas, permitindo uma melhor priorização das ações de combate e redução de perdas reais (físicas) e perdas aparentes (comerciais). Para o desenvolvimento desta metodologia, foi realizada uma ampla revisão bibliográfica, onde é apresentada a conceituação geral sobre perdas de água, suas causas e ocorrências, indicadores, ações para redução, e uma abordagem detalhada quanto às metodologias existentes para avaliação de perdas e ferramentas disponíveis para isto. A metodologia aqui aplicada desenvolve a avaliação das perdas através do balanço hídrico, utilizando o software gratuito desenvolvido pelo Banco Mundial (W-B Easy Calc - v1.17), e foi aplicada no sistema de abastecimento de água da Região Metropolitana de São Paulo (RMSP). Foram levantados os dados básicos de controle do sistema e realizados ensaios de campo, com medições de pressão e ensaios para determinação das perdas aparentes. Como estudo de caso, a metodologia foi aplicada em três setores de abastecimento da área central da RMSP, com características distintas. Os resultados do diagnóstico das perdas foram coerentes com as expectativas. Em função dos resultados, em cada setor deverá ser desenvolvida uma estratégia específica para a redução de perdas. / This study presents a discussion of a methodology for diagnosis and actions to reduce water losses, to assist in determining the profile of water losses, allowing a better prioritization of actions to combat and reduce real (physical) losses and apparent (commercial) losses. For developing the methodology, it was conducted an extensive bibliography review, where is presented the general concepts on water losses, its causes and events, indicators, actions to reduce, and a detailed discussion about the existing methodologies to assess losses and tools available for this. The applied methodology consists of an assessment of losses through the water balance, using free software developed by the World Bank (WB Easy Calc - v1.17) and it was applied in the water supply system in metropolitan region of Sao Paulo. To do this, it was necessary to collect basic data of system control and field tests, with pressure measurements and tests to determine apparent losses. As case study, the methodology was applied in three supply zones at the central area of metropolitan region of Sao Paulo, with different characteristics. The results of water losses diagnosis were consistent with expectations. With these results, a specific strategy should be developed in each sector for reducing losses.

Estações de tratamento de água que tratam águas com cor elevada, causada pela presença de substâncias húmicas, e baixa turbidez têm apresentado problemas operacionais, principalmente na coagulação. As substâncias húmicas aquáticas (SHA) apresentam moléculas de massa molecular variada, grande parte entre 500 Da e 10 kDa. Por esse motivo, as condições de coagulação (tipo e dosagem de coagulante e pH de coagulação) podem ser totalmente distintas no tratamento de águas com a mesma coloração, devido à presença de SHA com propriedades estruturais diferentes, conforme já observado em pesquisas recentes. Neste contexto, o presente trabalho estudou a influência do tamanho molecular aparente das SHA na coagulação por hidroxicloreto de alumínio e fez a comparação dos custos entre este coagulante e dois estudados anteriormente - sulfato de alumínio e cloreto férrico - para mesma eficiência de remoção de cor. Para isso, realizaram-se ensaios de coagulação seguida de filtração em jarteste com quatro águas de estudo preparadas com frações de diferentes tamanhos moleculares aparentes de SHA, com cor verdadeira de 100 uH e turbidez de 5,0 uT. A partir dos resultados encontrados, pode-se afirmar que há influência do tamanho molecular aparente de diferentes frações de substâncias húmicas aquáticas na coagulação por hidroxicloreto de alumínio. Observou-se a tendência de maior consumo de coagulante para a coagulação de frações de SHA de menores tamanhos moleculares aparentes, em uma faixa determinada de pH. Em relação aos custos, o cloreto férrico foi o coagulante que apresentou menor valor para tratamento (por filtração direta) de águas com elevada cor aparente devida a presença de SHA. / The water treatment plants that treat waters with high brown color caused by the presence of humic substances, and low turbidity have been presenting operational problems, mostly in the coagulation. The aquatic humic substances (AHS) present molecules of different molecular mass, most part between 500 Da and 10 kDa. Therefore, the coagulation terms (coagulant type and dosage and coagulation pH) can be totally distinct in waters treatment with the same coloration, due to the presence of AHS with different structural properties, as already observed in recent researches. In this context, the present work presented the influence of AHS\'s apparent molecular size in the coagulation for aluminum hydroxychloride and did the costs comparison between this coagulant and two studied previously - aluminum sulfate and ferric chloride - for same efficiency of the color removal. For that, it accomplished coagulation rehearsals followed by filtration in jar-test with four waters of study prepared with fractions of different AHS\'s apparent molecular sizes, with true color of 100 uH and turbidity of 5.0 uT. From the found results, it can affirm that there is influence of the apparent molecular size of different fractions from aquatic humic substances in the coagulation for aluminum hydroxychloride. The larger coagulant consumption tendency was observed for the AHS\'s fractions coagulation of smaller apparent molecular sizes, in a range determined by pH. Regarding the costs, the ferric chloride was the coagulant that presented smaller value for treatment (for direct filtration) of waters with high brown apparent color due the presence of AHS.