Les huiles essentielles sont connues pour leur richesse en molécules chirales. L'identification et la caractérisation de ces différentes molécules en termes de la configuration absolue des énantiomères majoritaires représente un important pas dans la compréhension des actions thérapeutiques des huiles essentielles. Dans le cadre de cette thèse, une étude est réalisée sur la composition chimique des huiles essentielles de quelques plantes aromatiques du Sahara algérien (l'Artemisia herba-alba, le Bubonium graveolens et l'Artemisia arborescens) ayant un usage fréquent dans la pharmacopée traditionnelle. Différentes techniques analytiques chromatographiques telles que la CG-SM classique et chirale, la CLHP chirale et spectroscopiques comme l'IR et le VCD seront mises en œuvre afin d’étudier les constituants chiraux de ces HE pour la connaissance de leurs signatures chiroptiques qui peuvent être des paramètres essentiels pour leurs caractérisations. En associant la performance du traitement chimiométrique, la fiabilité des techniques spectroscopiques et le potentiel discriminant de la signature chirale, nous avons développé des outils de caractérisation, de contrôle qualité et de traçabilité des HE. Les configurations absolues de la (-)-α-thujone, la (+)-β-thujone, l'acetate de (-)-cis-chrysanthenyl, le (+)-oxocyclonerolidol et l'acetate de (-)-cis-acetoxychrysanthenyl ont été obtenues par la comparaison des spectres VCD expérimentaux et calculés et on a montré qu'on peut utiliser le VCD pour l'étude et la modélisation des matrices complexes. / Essential oils are known for their richness in Chiral molecules. Identification and characterization of these different molecules in terms of absolute configuration of the majors enantiomers represents an important step in the understanding of the therapeutic actions of essential oils. In this thesis, a study was done to investigate the chemical composition of the essential oils of some aromatic plants of the Algerian Sahara (Artemisia herba-alba, Bubonium graveolens and Artemisia arborescens) frequently used in the traditional pharmacopoeia. Different chromatographic analytical techniques such as classic and chiral GC-MS, chiral HPLC and spectroscopic techniques as IR and VCD will be implemented to study the chiral constituents in these EO for the knowledge of their chiroptical signatures which can be essential parameters for their characterizations. Combining chemometrics processing performance, reliability of spectroscopic techniques and potential discriminating chiral signature, we have developed tools for the characterization, quality control and traceability of EO. Absolute configuration of (-)-α-thujone, (+)-β-thujone, (-)-cis-chrysanthenyl acetate, (+)-oxocyclonerolidol and (-)-cis-acetoxychrysanthenyl acetate were obtained by comparison of calculated and experimental VCD spectra and we demonstrated that VCD can be used for the study and modeling of complex matrices.

Sixteenth- and seventeenth-century portraits from the Italian peninsula that depict women with keyboard instruments have been discussed as an apparent trend by feminist art historians and musicologists. While the connection between these portraits and the well-known iconography of the musical St. Cecilia has been noted, the association between keyboard instruments and the female body has been less frequently explored. In this study, I use methodologies from feminist theory and gender studies, most notably gender performativity, in order to explore how an artist's dialogue between the portrait subject and her instrument creates and is created by complex relationships ingrained by the dominant patriarchal structures that circumscribed women's lives at the time. To realize these interpretive goals, I have chosen two paintings that are less often discussed in art historical and musicological literature: the self-portrait attributed to Marietta Robusti, and St. Cecilia Playing the Keyboard in the style of Artemisia Gentileschi.

A Artemisia annua L. (Asteraceae) é uma planta herbácea, nativa da Ásia e aclimatada no Brasil. As folhas apresentam até 1,4% do peso seco em artemisinina, sendo fonte abundante desta lactona sesquiterpênica que, conjuntamente aos seus derivados semi-sintéticos, apresentam ação efetiva contra as cepas resistentes das espécies de Plasmodium causadoras da malária. A artemisinina também está sendo testada com sucesso no tratamento de câncer e apresenta-se efetiva contra inúmeros parasitas que afetam a saúde humana e animal. Os objetivos deste trabalho foram determinar as épocas de plantio e colheita da cultivar Artemis de A. annua em Pato Branco-PR, como também, determinar em campo o fotoperíodo crítico e o número de ciclos fotoindutivos necessários para o florescimento desta cultivar. Um segundo experimento foi realizado com o objetivo de determinar o potencial alelopático desta espécie sobre sementes de alface e leiteiro. No experimento em campo, os tratamentos utilizados foram seis diferentes épocas de plantio, sendo as 09 plantas centrais das parcelas colhidas para a determinação da massa seca de folha e caules, relação folha/caule e teor de artemisinina na massa seca de folhas quando 50% das plantas da parcela iniciaram a emissão dos botões florais. O número de ciclos fotoindutivos encontrado para a cultivar Artemis foi de aproximadamente 36 ciclos e o fotoperíodo crítico aproximado foi de 13 horas, que ocorreu em 09/02/2007 em Pato Branco-PR. Esse resultado determinou que a época de colheita para a cultivar Artemis, na região de Pato Branco-PR, deve ser na segunda quinzena do mês de março. O maior rendimento de biomassa nesse experimento ocorreu no plantio de 27/07/2007, 231 dias após o transplantio, mas recomenda-se tomar cuidado com as geadas que podem ocorrer até a primeira quinzena de setembro, sugerindo-se optar pelo plantio na segunda quinzena de setembro, período livre de geadas na região. Os maiores teores de artemisinina (em média 0,80% na matéria seca de folhas) foram obtidos nas épocas que mais vegetaram a campo devido à maior produção de biomassa. Para o experimento de alelopatia, o extrato aquoso bruto foi preparado a partir de folhas frescas na proporção de 1 L de água destilada para 250 g do material. Foram utilizadas as seguintes concentrações do extrato aquoso: 100%, 75%, 50%, 25% e 0% (água destilada). Foram analisadas as variáveis germinabilidade, velocidade média de germinação, tempo médio de germinação, comprimento da radícula e massa seca das plântulas. Extratos aquosos de A. annua apresentaram ação alelopática inibitória sobre a germinação de sementes e o desenvolvimento de plântulas de alface e leiteiro. / The Artemisia annua L. (Asteraceae) is a herbaceous plant, native of Asia and acclimated in Brazil. The leaves are abundant source of artemisinin, a sesquiterpene lactone that, jointly to yours derived semi-synthetic, they present effectiveness action against the resistant stumps of the species of Plasmodium against malaria. The artemisinin is also being tested with success in the cancer treatment and against countless parasites that affect the human and animal health. The objectives of this work went to determine the planting times and crop of the cultivar Artemis of A. annua in Pato Branco-PR, as well as, to determine in field the critical photoperiod and the number of inductive photoperiod for the flowering of this cultivar. A second experiment was accomplished with the objective of determining the allellopathic effect of this species on lettuce seeds and wild poinsettia. In the experiment in field, the treatments were six different transplant times, being the 09 central plants of the portions picked for the determination of the mass dries of leaf and stems, relationship leaf/stems and artemisinin in the mass dries of leaves when 50% of the plants of the portion began the emission of the floral buttons. The number of cycles inductive found for the cultivar Artemis was of approximately 36 cycles and the critical photoperiod was approximately 13 hours, that happened in 09/02/2007 in Pato Branco-PR. This results determines that the crop time for cultivar Artemis, in Pato Branco-PR will be in the second fortnight of the month of March. The largest biomass income in that experiment happened in the planting of 27/07/2007, 231 days after the transplant, but it is recommended to take care with the frost that can happen until the first fortnight of September, suggesting choose for the transplant in the second fortnight of September, period free from frost in the area. The largest artemisinin tenor (0,88% in dry matter leaves) was obtained in the times that more they vegetated to field due to the largest biomass production. Besides this experiment, a second experiment was developed to determine the allellopathic effects of this species on lettuce seeds and wild poinsetia. The aqueous extract was prepared starting from fresh leaves in the proportion of 1 L of water distilled for 250 g of the material, after, the extract was filtrate and centrifuged, being used the sobrenadante. The following concentrations of the extract were used: 100%, 75%, 50%, 25% and 0% (distilled water). They were analyzed the variables germinability, germination velocity, germination time, length of the radicle and weight of the dry matter of the seedlings. Aqueous extracts of A. annua is allellopathic on the germination and development of lettuce and wild poinsettia seedlings.

A Artemisia annua L. (Asteraceae) é uma planta herbácea, nativa da Ásia e aclimatada no Brasil. As folhas apresentam até 1,4% do peso seco em artemisinina, sendo fonte abundante desta lactona sesquiterpênica que, conjuntamente aos seus derivados semi-sintéticos, apresentam ação efetiva contra as cepas resistentes das espécies de Plasmodium causadoras da malária. A artemisinina também está sendo testada com sucesso no tratamento de câncer e apresenta-se efetiva contra inúmeros parasitas que afetam a saúde humana e animal. Os objetivos deste trabalho foram determinar as épocas de plantio e colheita da cultivar Artemis de A. annua em Pato Branco-PR, como também, determinar em campo o fotoperíodo crítico e o número de ciclos fotoindutivos necessários para o florescimento desta cultivar. Um segundo experimento foi realizado com o objetivo de determinar o potencial alelopático desta espécie sobre sementes de alface e leiteiro. No experimento em campo, os tratamentos utilizados foram seis diferentes épocas de plantio, sendo as 09 plantas centrais das parcelas colhidas para a determinação da massa seca de folha e caules, relação folha/caule e teor de artemisinina na massa seca de folhas quando 50% das plantas da parcela iniciaram a emissão dos botões florais. O número de ciclos fotoindutivos encontrado para a cultivar Artemis foi de aproximadamente 36 ciclos e o fotoperíodo crítico aproximado foi de 13 horas, que ocorreu em 09/02/2007 em Pato Branco-PR. Esse resultado determinou que a época de colheita para a cultivar Artemis, na região de Pato Branco-PR, deve ser na segunda quinzena do mês de março. O maior rendimento de biomassa nesse experimento ocorreu no plantio de 27/07/2007, 231 dias após o transplantio, mas recomenda-se tomar cuidado com as geadas que podem ocorrer até a primeira quinzena de setembro, sugerindo-se optar pelo plantio na segunda quinzena de setembro, período livre de geadas na região. Os maiores teores de artemisinina (em média 0,80% na matéria seca de folhas) foram obtidos nas épocas que mais vegetaram a campo devido à maior produção de biomassa. Para o experimento de alelopatia, o extrato aquoso bruto foi preparado a partir de folhas frescas na proporção de 1 L de água destilada para 250 g do material. Foram utilizadas as seguintes concentrações do extrato aquoso: 100%, 75%, 50%, 25% e 0% (água destilada). Foram analisadas as variáveis germinabilidade, velocidade média de germinação, tempo médio de germinação, comprimento da radícula e massa seca das plântulas. Extratos aquosos de A. annua apresentaram ação alelopática inibitória sobre a germinação de sementes e o desenvolvimento de plântulas de alface e leiteiro. / The Artemisia annua L. (Asteraceae) is a herbaceous plant, native of Asia and acclimated in Brazil. The leaves are abundant source of artemisinin, a sesquiterpene lactone that, jointly to yours derived semi-synthetic, they present effectiveness action against the resistant stumps of the species of Plasmodium against malaria. The artemisinin is also being tested with success in the cancer treatment and against countless parasites that affect the human and animal health. The objectives of this work went to determine the planting times and crop of the cultivar Artemis of A. annua in Pato Branco-PR, as well as, to determine in field the critical photoperiod and the number of inductive photoperiod for the flowering of this cultivar. A second experiment was accomplished with the objective of determining the allellopathic effect of this species on lettuce seeds and wild poinsettia. In the experiment in field, the treatments were six different transplant times, being the 09 central plants of the portions picked for the determination of the mass dries of leaf and stems, relationship leaf/stems and artemisinin in the mass dries of leaves when 50% of the plants of the portion began the emission of the floral buttons. The number of cycles inductive found for the cultivar Artemis was of approximately 36 cycles and the critical photoperiod was approximately 13 hours, that happened in 09/02/2007 in Pato Branco-PR. This results determines that the crop time for cultivar Artemis, in Pato Branco-PR will be in the second fortnight of the month of March. The largest biomass income in that experiment happened in the planting of 27/07/2007, 231 days after the transplant, but it is recommended to take care with the frost that can happen until the first fortnight of September, suggesting choose for the transplant in the second fortnight of September, period free from frost in the area. The largest artemisinin tenor (0,88% in dry matter leaves) was obtained in the times that more they vegetated to field due to the largest biomass production. Besides this experiment, a second experiment was developed to determine the allellopathic effects of this species on lettuce seeds and wild poinsetia. The aqueous extract was prepared starting from fresh leaves in the proportion of 1 L of water distilled for 250 g of the material, after, the extract was filtrate and centrifuged, being used the sobrenadante. The following concentrations of the extract were used: 100%, 75%, 50%, 25% and 0% (distilled water). They were analyzed the variables germinability, germination velocity, germination time, length of the radicle and weight of the dry matter of the seedlings. Aqueous extracts of A. annua is allellopathic on the germination and development of lettuce and wild poinsettia seedlings.

Magister Pharmaceuticae - MPharm / Leonotis leonurus (L. leonurus) and Artemisia afra (A. afra) are two of the most commonly used medicinal plants in South Africa traditionally advocated for use in asthma. However, proper scientific studies to validate these claimed uses are lacking and little is known about the mechanisms for this effect. These plants contain flavonoids, which are reported to have smooth muscle relaxant activity and may be responsible for the activity of these two plants. The objectives of this study were to: (1) determine and compare the flavonoid profiles and levels in A. afra and L. leonurus, (2) compare the respiratory smooth muscle relaxant effects of freeze-dried aqueous extracts of A. afra and L. leonurus and (3) investigate whether K⁺ - channel activation (i.e. KATP channel) is one possible mechanism of action that can explain the effect obtained in traditional use of these two plants. It was hypothesized that: (1) the flavonoid levels and profile of A. afra would be greater than the flavonoid levels and profile of L. leonurus, (2) A. afra would have a more potent respiratory muscle relaxant effect than L. leonurus and (3) A. afra and L. leonurus will inhibit K⁺ - induced contractions in a superior manner than carbachol and histamine - induced contractions. To realize these objectives, freeze-dried aqueous extracts (FDAE) of the dried leaves of the two plants were prepared. A validated HPLC assay was developed and used to identify and determine the levels of luteolin in the plant preparations. Solutions of the plant extracts were studied in the isolated guinea-pig trachea tissue preparation in the presence of carbachol, histamine and KCL. The possible mechanism of action of the two plants was determined by cumulative log dose-response curves (LDRC) for carbachol, histamine and KCL in the absence and presence of 1, 30 and 100 mg/ml solutions of the plant extracts. The flavonoid profile of un-hydrolyzed and hydrolyzed L. leonurus was greater than that of un-hydrolyzed and hydrolyzed A. afra. The levels of free and total luteolin in A. afra FDAE (8.977 ± 0.73 μg/ml and 16.394 ± 0.884 μg/ml, respectively) were significantly (p < 0.001) higher than that in L. leonurus FDAE (0.929 ± 0.066 μg/ml and 3.093 ± 0.531 μg/ml, respectively). L. leonurus and A. afra relaxed tracheal smooth muscles contracted with histamine, KCL and carbachol in a dose dependent manner. The degree of relaxant activity of L. leonurus versus the three inducers of contraction (agonists) could be classified as KCL > carbachol > histamine, with EC₅₀ values of 9.87, 29.34 and 94.76 mg/ml, respectively. The A. afra tracheal smooth muscle relaxant activity was categorized as carbachol > histamine > KCL, with EC₅₀ values of 13.93, 15.47 and 19.88 mg/ml, respectively. Overall, A. afra which contained the higher levels of luteolin, was more potent at relaxing the guinea pig tracheal smooth muscle than L. leonurus. Collectively, the results confirm that aqueous solutions of A. afra and L. leonurus as used in local traditional practice have potent but different degrees of bronchodilator activities that could be useful in the treatment of asthma, and that these actions may be related to each plant's luteolin (or flavonoid) levels. Moreover it is very unlikely that KATP channels are primarily responsible for the actions of A. afra and L. leonurus, but rather that more than one mechanism of action is involved in the tracheal smooth muscle relaxant effects of these two plants. / National Research Foundation

>Magister Scientiae - MSc / Current research classifies Type 2 diabetes as most prevalent non-communicable diseases in South Africa. Approximately 285 million people are affected globally with an expected increase to 595 million by the year 2035. Synthetic first-line drugs in the treatment of Type 2 diabetes, have been shown to have an efficacy rate of approximately 43% as a result of poor drug uptake and metabolism. Furthermore, given South Africa’s uniquely diverse botanical heritage, herbs commonly used traditional medicine have shown promise in the treatment of Type 2 diabetes.

Malaria, a disease caused by parasites of the Plasmodium genus, infects over 220 million people annually, resulting in over 400,000 deaths. Most of these deaths occur in Africa in children < 5 years of age. Artemisia annua L., an ancient Chinese medicinal herb, is known for its foremost phytochemical constituent, artemisinin (AN). Semisynthetic derivatives of AN form the primary component of artemisinin combination therapies (ACTs), the frontline treatment for malaria worldwide. However, ACTs have several drawbacks including cost and availability. Thus, cheaper, more readily available antimalarials are needed. Recent clinical data suggested dried leaves of A. annua (DLA) administered orally as a tea infusion may be as efficacious as ACTs despite a significantly lower AN dose delivered. In mice, AN plasma concentration was improved when administered as DLA compared to pure AN. I therefore hypothesized that phytochemicals within DLA enhanced the oral bioavailability of AN. To investigate this hypothesis, here I examined the effects of DLA on the underlying mechanisms that govern oral bioavailability. Using an in vitro human digestion model, I showed that AN solubility was greater when delivered as DLA, largely due to essential oil in the plant. Furthermore, AN intestinal permeability was enhanced in a Caco-2 cell model of the intestinal epithelium. Extracts, teas, and phytochemicals produced by Artemisia also inhibited the activity of CYP2B6 and CYP3A4, the enzymes responsible for first-pass AN metabolism in the liver. Additionally, AN tissue distribution was improved when delivered as DLA and AN accumulation in tissues was higher in female vs. male rats. Finally, I showed that DLA was a more efficacious anti-inflammatory than pure AN in rats, potentially due to enhanced AN bioavailability. Taken together, these results shed light on the mechanisms behind enhanced oral bioavailability afforded by DLA and demonstrate the potential for DLA to be used as a therapeutic for malaria and other diseases.

Critical mule deer (Odocoileus hemionus) winter ranges in southeastern Utah dominated by Wyoming big sagebrush (Artemisia tridentata var. wyomingensis [Beetle and A. Young] Welsh) have developed dense cheatgrass (Bromus tectorum L.) understories. These communities are currently characterized by predominately mature to decadent stands of sagebrush with few perennial grasses and forbs. Sagebrush seedlings and perennial grasses compete for limited resources with annual grasses and forbs. To determine the affects of cheatgrass control on sagebrush growth and reproductive characteristics, imazapic (PLATEAU®, AC 263,222) herbicide was sprayed at 438.5 ml/ha with water and methylated seed oil during active fall growth of cheatgrass in 2002. Sagebrush growth and reproductive variables were measured on browsed and unbrowsed (caged) plants on sprayed and non-sprayed paired plots on 6 sites. Cheatgrass and other annual forb pretreatment cover was reduced from 23% to less than 3% the first year after the herbicide treatement and only increased to 4% the second year. Soil moisture on the treated plots was available at 15, 30, and 60 cm for several days to several weeks longer than on the control plots depending on the depth in the soil and year. Annual leader growth and flowering seedstalk length were similar on unbrowsed and browsed shrubs, but the number of seedstalks per plant was decreased by browsing. The number of sagebrush flowering seedstalks was significantly reduced by the herbicide the first year after the treatment, but recovered by the second year. The decrease in flowering seedstalks per sagebrush reduced the number of sagebrush seedlings observed the second year after the treatment (control = 81,800 seedlings/ha and treated = 16,700 seedlings/ha). Both seedstalk length (treated = 13.4 cm and control = 11.2 cm) and annual leader growth (treated = 6.2 cm and control = 5.3 cm) were greater on treated plots than control plots. Overall, imazapic can provide a window of cheatgrass and annual forb control to allow big sagebrush seedlings and perennial grasses and forbs to establish.

Yes / Ancient Chinese herbal texts as far back as the 4th Century Zhou hou bei ji fang describe methods for the use of Qing Hao (Artemisia annua) for the treatment of intermittent fevers. Today, the A. annua constituent artemisinin is an important antimalarial drug and the herb itself is being grown and used locally for malaria treatment although this practice is controversial. Here we show that the ancient Chinese methods that involved either soaking, (followed by wringing) or pounding, (followed by squeezing) the fresh herb are more effective in producing artemisinin-rich extracts than the usual current method of preparing herbal teas from the dried herb. The concentrations of artemisinin in the extracts was up to 20-fold higher than that in a herbal tea prepared from the dried herb, but the amount of total artemisinin extracted by the Chinese methods was much less than that removed in the herbal tea. While both extracts exhibited potent in vitro activities against Plasmodium falciparum, only the pounded juice contained sufficient artemisinin to suppress parasitaemia in P. berghei infected mice. The implications of these results are discussed in the context of malaria treatment using A. annua infusions.

Submitted by Renata Lopes (renatasil82@gmail.com) on 2016-09-23T14:40:10Z No. of bitstreams: 1 luisamariasilveiradealmeida.pdf: 3706782 bytes, checksum: 1b4e01bcd663e5b0167c387beb0d0e2d (MD5) / Approved for entry into archive by Diamantino Mayra (mayra.diamantino@ufjf.edu.br) on 2016-09-26T20:31:05Z (GMT) No. of bitstreams: 1 luisamariasilveiradealmeida.pdf: 3706782 bytes, checksum: 1b4e01bcd663e5b0167c387beb0d0e2d (MD5) / Made available in DSpace on 2016-09-26T20:31:05Z (GMT). No. of bitstreams: 1 luisamariasilveiradealmeida.pdf: 3706782 bytes, checksum: 1b4e01bcd663e5b0167c387beb0d0e2d (MD5) Previous issue date: 2015-07-30 / CAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior / A esquistossomose é uma doença que afeta cerca de 200 milhões de pessoas no mundo e mais de 600 milhões estão expostas ao risco de contraí-la, sendo que no Brasil, o Estado de Minas Gerais é um dos mais afetados com esta parasitose. Atualmente, o único fármaco disponível para o tratamento desta doença é o praziquantel (PZQ). No entanto, este medicamento já está no mercado há décadas e tem demonstrado relatos de ineficácia. Neste contexto, extratos e metabólitos secundários obtidos de espécie do gênero Artemisia (Asteraceae) têm demonstrado expressiva atividade esquistossomicida. Assim, o presente trabalho descreve o estudo fitoquímico do extrato diclorometânico das folhas de Artemisia absinthium L. (AAF), bem como a avaliação da atividade esquistossomicida in vitro dos extratos AAF e das raízes, do óleo essencial e do metabólito obtido. O estudo fitoquímico, do AAF, culminou no isolamento e identificação de dois metabólitos: uma flavona, artemetina e uma lactona sesquiterpênica, hidroxipelenolídeo. No estudo esquistossomicida in vitro foi utilizado vermes adultos de S. mansoni da linhagem BH e os parâmetros avaliados foram mortalidade, motilidade e alterações tegumentares. Os resultados deste estudo demonstraram que o extrato diclorometânico bruto (AAF), na concentração de 200 μg/mL, possui expressiva atividade esquistossomicida in vitro ao ser capaz de causar a morte de 100% dos parasitos após 24 h de incubação. Em relação à atividade esquistossomicida do extrato bruto da raiz (200 μg/mL), este foi capaz de provocar a morte de todos os vermes após 48 h. O óleo essencial da A. absinthium L. (100 µg/mL) apresentou atividade esquistossomicida moderada sendo capaz de causar 60% de mortalidade nos parasitos após 48 h de incubação. Dentre as substâncias isoladas, a artemetina, foi submetida ao ensaio de atividade esquistossomicida e não foi ativa. Estudos adicionais de avaliação esquistossomicida devem ser realizados com o extrato bruto e metabólitos de A. absinthium L. com o intuito de se identificar as moléculas responsáveis pela atividade esquistossomicida in vitro observada pelo extrato AAF. / Schistosomiasis is a disease that affects more than 200 million individuals in the world and 600 million people remain under risk of infection. In Brazil, the Minas Gerais state is one of the most affected by this disease. Nowadays, the only drug available for schistosomiasis treatment is praziquantel (PZQ). However, this drug is already on market for decades and has shown ineffectiveness. In this context, extract and secondary metabolites of the genus Artemisia (Asteraceae) have shown an expressive schistosomicidal activity. Thus, the present work describes the phytochemical study of the dichloromethane extract of Artemisia absinthium L. leaves (AAF), as well as in vitro schistosomicidal evaluation of AAF, crude roots extracts, essential oil and metabolite. The phytochemical study of AAF allowed the isolation and identification of two metabolites: one flavone, artemetin and one sesquiterpene lactone, hydroxypelenolide. In the in vitro schistosomicidal study, was used adult worms of the S. mansoni BH strain and the parameters evaluated were mortality, motility and alterations tegumental surface. The results showed that AAF, at 200 μg/mL, presents expressive schistosomicidal activity being able to cause 100% of death in parasites after 24 h of incubation. Regarding the schistosomicidal activity of the extract crude roots extract (200 μg/mL), was able to kill all the parasites after 48 h. The A. absinthium L. essential oil (100 mg / mL) showed moderate schistosomicidal activity being able to cause 60% mortality of parasites after 48 h of incubation. Among the isolated compounds, artemetin, was subjected to the schistosomicidal activity test and was inactive. Additional schistosomicidal assays should be carried out with A. absinthium L. crude extract and metabolites in order to identify the molecules responsible for schistosomicidal in vitro activity observed by AAF.