Here we report the preparation, structure, and spectroscopic properties of an intriguing copper(I) cyclophane-like dimeric complex [Cu2(dppa)2(bpy)2][BF4]2(2)¡B[Cu2(dppa)2(mbpy)2][BF4]2(3)¡B[Cu2(dpbp)2(bpy)2][BF4]2(7)©M[Cu2(dppb)2(bpy)2] [BF4]2(8), containing phenyl phosphine bridge ligands, such as diphenylphosphino acetylene (dppa), 1,4-bis(diphenylphosphino)benzene (dppb) and 4,4'-bis(diphenyl phosphino)biphenyl (dpbp). As a building unit, the complex [Cu2(dpbp)2(NCMe)4] [BF4]2(9) containing labile acetonitrile molecules those can be easily substituted by anionic ligands, is expected to combine with suitable linkers to synthesize supramolecular arrays with shapes of polygons and polyhedra.

The Human Genome Project completed in 2003 and the draft of human genome sequences were also yielded. It has been known that any two human gnomes are almost identical, and only very little difference makes human diversities. Single nucleotide polymorphism (SNP) means that a single-base nucleotide changes in DNA. A SNP sequence from one of a pair of chromosomes is called a haplotype. In this thesis, we study how to reconstruct a pair of chromosomes from a given set of fragments obtained by DNA sequencing in an individual. We define a new problem, the chromosome pair assembly problem, for the chromosome reconstruction. The goal of the problem is to find a pair of sequences such that the pair of output sequences have the minimum mismatch with the input fragments and their lengths are minimum. We first transform the problem instance into a directed multigraph. And then we propose an efficient algorithm to solve the problem. We apply the ACO algorithm to optimize the ordering of input fragments and use dynamic programming to determine SNP sites. After the chromosome pair is reconstructed, the two haplotypes can also be determined. We perform our algorithm on some artificial test data. The experiments show that our results are near the optimal solutions of the test data.

Taiwan¡¦s monopoly system, originating from the Military Governor Ming-chuan Liu of Qing Dynasty and applied by the Japanese, was a major financial source in Japan-colonized Taiwan. Chen Yi, the first ROC Chief Executive and Garrison Commander of Taiwan, renamed the Monopoly Bureau of Government-General as the Monopoly Bureau of Taiwan and then continued its monopolistic business. He stipulated five items -- tobacco, alcohol, camphor, matches, and measurement gadgets-- be monopolized by government whereas private manufacturing and sales be barred under the supervision of the Monopoly Bureau. Also banned from private transactions are certain necessities, such as salt, lime, cane sugar, gasoline, and electricity, which were distributed by some authorities other than the Monopoly Bureau. It was thought that such measures could restrict individual capital, and reinforce national capital in the hopes of realizing the idea of the nation founding, based on the doctrines of the Three People¡¦s Principles. Dr. Sun Yat-sen, though asserting capital restrictions in some degree, was not opposed to private capital, but, rather, was keen to encourage private enterprises. He had the national industries developed by a dual action via both private enterprises and national organizations. His philosophy argued that those productions not so proper to be commissioned to a state-run organization as to a private one should, along with incentives from the state and protection by the law, be rendered to the latter for operations. Based on this, it appeared obvious that people¡¦s livelihood didn¡¦t connote nationalization. And by no means did livelihood denote that the government could confiscate the industries already operated by civilians. After a further study, a distinction could be found in the argument between the economic centralization implemented by the authorities of Taiwan¡¦s administrative officers and the capital restriction elaborated in the Principles of People¡¦s Livelihood. The colossal assets taken over from Japanese-owned industries didn¡¦t mean any opportunities to reinforce national capital. Instead, monopolistic business was designed to operate by the government and afterwards turned up being outstanding samples of state-owned businesses among the industries in post-war Taiwan. The key factor consisted in the fact that state-owned industries were run under the supervision of the Taiwan Provincial Assembly, which boosted the managerial performance. The revenues from monopolistic sales of tobacco and spirits were crucial to the finances of our country and were helpful to the development of its economic constructions. The monopoly featured two functions. One function was to revive the production of the state-owned industries, which laid a foundation to carry out a six-term Four-year Economic Program, beginning in 1953; to make a substitute for imports; to spur export expansion; and to help kick-start our country¡¦s economic boom. The other function was to improve village constructions with land reforms of Three-seven-five Rent Deduction (a program limiting the rent of a land to a maximum of 37.5 percent of its total product), Release of State Lands, and Land-to-the-tillers Movements. The monopoly system was an indispensable support of finances, and it also ensured that the capital from the agricultural sector could be transferred to the industrial sector, which proved that provincial constructions were closely related to national economic development.

This study is focused on two-sided assembly line balancing problems of type-I and type-II. This problem is encountered in production environments where a two-sided assembly line is used to produce physically large products. For type-I problems, there is a specified production target for a fixed time interval and the objective is to reach this production capacity with the minimum assembly line length used. On the other hand, type-II problem focuses on reaching the maximum production level using a fixed assembly line and workforce. Two different mathematical models for each problem type are developed to optimally solve the problems. Since the quality of the solutions by mathematical models decreases for large-sized problems due to time and memory limitations, two heuristic approaches are presented for solving large-sized type-I problem. The validity of all formulations is verified with the small-sized literature problems and the performances of the methods introduced are tested with large-sized literature problems.

In order to meet production requirements of small quantity and large variety for versatile market demands, industrial robots with dexterous end-effectors are usually applied to the flexible manufacturing systems. However, owing to constraints of robot¡¦s accuracy, repeatability, and resolution, assembled parts may experience collision during the insertion process. Both positional and angular errors, which cannot be easily predicted because of indeterminate collision situations, may cause failure of the assembly. One of the frequently applied strategies is to use a passive remote center compliance device. Most traditional remote center compliance (RCC) devices aim to solve insertion difficulty for round peg insertion. This dissertation is devoted to analyze the insertion behavior and develop a new remote center compliance device for square pegs, which lack of the axial symmetry property of round pegs. The presented Passive Multiple Remote Center Compliance Device (MRCC) introduces a new azimuthal compliance over traditional passive compliance mechanisms that can effectively compensate the peg¡¦s orientation deviation for polygonal assembly. Besides, a special feature of the adjustable compliance provides capability to overcome the gravity effect. Non-vertical insertions therefore become possible. A spring-supported object in space is also adopted for stability analysis of the compliant device. Actual experimental assembly processes demonstrate promising results on polygonal insertions in both traditional top-down and horizontal directions. The assembly process of a square peg consists of approach, one-point contact, two-point contact, three-point contact, four-point contact, rotation, departure from chamfer crossing, and insertion. Full analysis of the square peg mating process, using a quasi-static approach will be presented. Constraints that can avoid the jamming and wedging phenomenon for successful assembly will also be established. Furthermore, a novel geometric insertion map, which is able to predict regions of failure and success before actual insertion takes place, is developed to improve efficiency of successful assembly for square pegs.

In this research we consider two different approaches for microfabricating physical templates to be used in template directed colloidal self-assembly experiments. Fabrication of templates, usable with confocal microscopy, forms an essential part of observation and analysis of template directed colloidal self-assembly studies. We use existing laboratory based microfabrication methods for patterning thin glass coverslips and polymeric films. These templates when used for directing colloidal self-assembly along with confocal microscopy analysis provide us with relevant information on the effect of confined geometries of the template on particle packing and order. The first method of template fabrication involves ultraviolet photolithography, thin film deposition, and glass micro machining. Various stages of the process were optimized while selecting reactive ion etch (RIE) and nickel etch mask with a suitable etch recipe for microfabrication of patterns on thin multi-component glass coverslips. Pattern dimensions were shown to be nearly commensurate with patterns on the microfiche, which was used as a field mask. In another approach, mechanical machining for fabricating polymeric templates was attempted on poly(methyl methacrylate) films spin coated on thin glass cover slips. The mechanical machining was implemented using computer numerical control (CNC) machines with the pattern dimensions in the range of 50 Mu m-150 Mu m. The glass and polymeric templates were used in template directed colloidal self-assembly experiments us ing polystyrene or silica particles. Confocal microscopy was used to obtain images of particle packing in template geometries. Imaging of the particles confined in the template geometries show increased particle concentration along pattern walls and corners. Inherent pattern irregularities and roughness possibly resulted in limited order in particle. Using a simple fortran program, image stack generated from confocal microscopy is used for obtaining images of particle packing in four different view planes which includes top, side, cross sectional and diagonal view of the image stack. The results from this research show the application of simple microfabrication processes for creating physical templates for template directed colloidal self-assembly. Confocal microscopy imaging combined with fortran image processing program can provide images of particle packing in different view planes. These images of the particles confined in various pattern geometries illustrate greater possibility of packing order in straight and regular pattern geometries or profiles.

This study presents a novel polyimide-based self-assembly three dimensional micro blade using surface micromachining technology for the development of micro-fan chip. The high surface-tension-force of reflowed polyimide has can be used to lift the free-standing micro blade. In addition, the thesis introduces a micro hinge structure to effectively limit the maximum lifting angle of the micro blade and to accurately lock hinge-pin into the vertical position. Many parameters have been investigated its influence on the surface-tension- force of polyimide, including the thickness of polyimide and the temperature/time in reflow processing. Based on the experimental results, 18 £gm-thick polyimide can lift the micro blade at 70¢X angle under 380 ¢J/10 hrs reflow condition. On the other hand, 25 £gm-thick polyimide has demonstrated its maximum lifting angle can be achieved to 130¢X utilizing the very high surface-tension-force induced by over contraction and deformation when it was reflowed at higher temperature (400 ¢J). Finally, this dissertation has studied the relation between the position of polyimide elastic-joint and the deflection angle (£r). Furthermore, this thesis has successfully demonstrated a novel multi-joint and asymmetrical microstructure for the development of the spiral and out-of-plane 3D micro blade.

The goal of this thesis is to develop a novel polishing tool system. This system can be attached to a CNC machine and execute a precision polishing job mainly for an axially symmetric free surface. The precision polishing job is to remove the error surface profile on the work to improve its form precision, which was left by the previous machining process. An inferential rule, which was based on a top-down planning strategy, was utilized to gradually decompose the design goals of the tool system to facilitate the process for generating all of the possible design proposals. The major design goal is to render all the rotational axes of the tool system to exactly intersect at the tool center. To analyze the effects of the structure and interface stiffness of tool system on the major goal, the finite element method was adopted. Further, the homogeneous transformation scheme is applied to establish the forward kinematic error of the designed system and to analyze the effect of different manufacturing and assembly errors on the major goal.Accordingly, two novel polishing tool systems were developed. The simulation study indicated that the total errors after assembly at the tool center and the two rotation axes were dominated by the stiffness at the interfaces of the tool system, in addition to the influence of structure stiffness. An assembly strategy was then proposed in the study to reduce the total error.

<p>Imagine if you could combine the power and capabilities ofan entire laboratory in the palm of your hand. Advances inmicrofluidic chip technology promise to integrate andminiaturize multiple lab processes into a single palm-sizeddevice. The advantages of these lab-on-a-chip devices,sometimes also referred to as micro total analysis systems(µTAS), compared with conventional bench-scale systems arenumerous and wide ranging and include: less reagentconsumption, low manufacturing costs, increased performance,faster analysis, high sample throughput, integration andautomation possibilities, and disposability. However,microfluidic devices also present challenges such as theinterfacing to the macro world and detection limits.</p><p>In this thesis the focus has been to develop novel discretemicrofluidic components for biotechnology and organic chemicalapplications with the goal to integrate them to formlab-on-chips. A flow-through filter-chamber device has beendesigned, manufactured and evaluated for chemical analysis onbeads. Passive liquid handling has been integrated on the chipin the form of hydrophobic valves at the inlet channels. Anarray format has also been developed to allow parallel analysisof multiple samples. The filter-chamber functions well forsingle nucleotide analysis using pyrosequencing. Initialevaluations on catalyst screening in the filter-chamber devicehas been performed.</p><p>The suitability of valve-less micropumps for biochemicalapplications is presented. Fluids encountered in variousbiochemical methods, including living cells, that areproblematic for other micropumps have been pumped with goodperformance. This thesis also introduces expandablemicrospheres as a novel component in microfluidics includingapplications such as one-shot valves, micropositioning andsurface enlargement.</p><p>A novel technique for bead immobilization in microfluidicdevices based on surface chemistry is presented in this thesis.Beads for both biochemical assays and organic chemistry havebeen self-sorted and self-assembled in line patterns as narrowas 5 µm on both structured and unstructured substrates.This method will greatly facilitate the generation of screeningplatforms, for example.</p><p>To develop a microfluidic device for catalysis-on-chip,ligands for asymmetric catalysis have successfully beenimmobilized in silicon channels by consecutive microcontactprinting, which is a novel technique presented in thisthesis.</p><p><b>Keywords:</b>microfluidics, beads, microspheres, silicon,filter-chamber, flow-through, bead trapping, DRIE, passivevalves, fluorocarbon, microfluidic array, adhesive bonding,valve-less micropump, microcontact printing, PDMS,self-assembly, self-sorting, DNA, SNP, pyrosequencing,allele-specific extension, expandable microspheres, catalysis,chiral ligand, monolayer, miniaturization, lab-on-a-chip,µTAS.</p>

<p>The work detailed in this dissertation relates to thedevelopment of high performance manufacturing systems. Theperformance factor aimed for is especially flexibility, butthere is an intention of making the results adaptable to focuson performance factors of the readers or users choice. Thefocus of the presented research is not only to provide meansfor accomplishing manufacturing that can handle changes butalso to accomplish flexibility in another area. The resultsshould be applicable in many different situations. The researchhas been divided into three parts: the further development of amanufacturing strategy, the development of a base for amanufacturing system design method and the development of amanufacturing control system.</p><p>The developed strategy is called Assembly-InitiatedProduction (AIP). An implementation of the strategy shouldprovide high manufacturing system flexibility but at the sametime contribute to the lowering of inventory levels andlead-times. Different solutions coupled to technicalrequirements found are also discussed.</p><p>The design method research focuses on basic manufacturingsystem properties and the possibility of expressing theseproperties by using simple combinable abstract units calledconcepts. The principle is the same as in physics where realworld phenomena may be expressed by using standard concepts asfor example time and mass. The intended use of the results isin an early manufacturing system design phase. The method isnot directly linked to the AIP strategy, but could be used forimplementing it.</p><p>Production Planning and Control (PPC) is an important partof a manufacturing system. After having reviewed current PPCpractices, a need for a factory floor PPC system workingregardless of factory floor layout, was identified. Based ontheoretical and industrial studies, the suggested solution is acomputerised, decentralised control system, physicallyseparated from the PPC/ERP system. In order to be able to makequick changes in the schedule, to obtain flexibility and toprovide the organisation with a tool for manufacturing controland decision-making, the system works in real time to provideaccurate and valid data.</p>