Objective: The cytotoxic property of anti-EGFR-1 monoclonal-antibody nimotuzumab modified with nuclear localization sequence and radiolabeled with 111In was evaluated in trastuzumab-resistant breast cancer cells. Methods: 111In-nimotuzumab-NLS was constructed and its immunoreactivity was determined. Cellular and nuclear uptake was evaluated by cell fractionation. Finally, the cytotoxicity of conjugates (111In-nimotuzumab/111In-nimotuzumab-NLS) was studied by clonogenic assays. Results: The immunoreactivity of 111In-nimotuzumab-NLS was conserved. 111In-nimotuzumab-NLS exhibited 2-fold higher nuclear translocation as compared to 111In-nimotuzumab in MDA-MB-468 cells. Nuclear importation of 111In-nimotuzumab-NLS in MDA-MB-468 cells was 4-fold and 6-fold higher than moderate and low EGFR expressing cell lines, respectively. Clonogenic survival (CS) for MDA-MB-468 cells showed 111In-nimotuzumab-NLS to be 10-folds and 60-folds more potent than 111In-nimotuzumab and nimotuzumab, respectively. Moderate killing for TrR1 and MDA-MB-231 was observed. 111In-hEGF showed significantly higher cytotoxicity and 2-fold higher γ-H2AX foci integrated density/nuclear-area as compared to 111In-nimotuzumab-NLS. Preserved selectivity of 111In-nimotuzumab-NLS makes it an excellent drug for treating cancers.

The subject of this theoretical  study is the role ofelectronic structure as well as of rotational and vibrational motionson interactions between molecules and electromagnetic radiation,ranging from optical to x-ray. The thesis concerns both linear and nonlinear regimes of the light-matter interaction. The first part of the thesis is devoted to propagation of opticalpulses with different time-structure through various nonlinear absorbers.First we explain the double-exponential decay of fluorescence caused by photobleaching of pyrylium  salt irradiated by a train of short (100 fs) optical pulses. The main reason for this effect is the transversal inhomogeneity of the light beam which makes the dynamics of the photobleaching differ in the core of the pulse and on its periphery. We also explore the optical power limitingof C60 fullerene irradiated by either microsecond optical pulses or a picosecond pulse trains. Enhancement of nonlinear absorption is caused by strong triplet-triplet absorption that becomes important due toelongation of the interaction time.Here we show the importance of the repetitionrate for the optical power limiting performance.The second part of the thesis addresses the interaction of optical and x-rayfields with rotational degrees of freedom of molecules. In this part the main attention is paid to the rotational heating caused by the recoil, experienced by molecules due to the ejection of photoelectrons. We have quantitatively explained two qualitatively different experiments with the N2 molecule.We predict the interference modulation of the recoil-induced shift,which is a shift of the photoelectron line caused by the rotational recoil effect, as a function of the photon energy.The developed theory also explains the rotational heating ofmolecules observed in the optical fluorescence induced by x-ray radiation.Based on this explanation, we suggest a new scheme of the optical fluorescence induced by x-rays that allows to detect the recoil effect via the recoil-inducedsplitting of the optical resonance.The last part of the thesis focuses on multi-mode nuclear dynamics of the resonant Auger scattering from the C2H2 molecule, that was the subject of a recent experimental study.Here we develop a theory that explains the observed vibrationalscattering anisotropy. We have found that three qualitatively different mechanisms are responsible for this phenomenon. The first mechanism is the interference of the direct and resonance scattering channels. The second mechanismis the interference of the resonant scattering channels through core excitedstate with the orthogonal orientation of the vibrational modes of core excitedstate. The Young's double slit like interference of the quantum pathways through the double-well potential of the bending motion of core excited state is the third mechanism of the vibrational scattering anisotropy. / QC 20100713

Darmstadt, Techn. Universiẗat, Diss., 2007. / Dateien im PDF-Format.

Chemnitz, Techn. Univ., Diss., 2007.

abstract: Trenchless technologies have emerged as a viable alternative to traditional open trench methods for installing underground pipelines and conduits. Pilot Tube Microtunneling, also referred to as the pilot tube system of microtunneling, guided auger boring, or guided boring method, is a recent addition to the family of trenchless installation methods. Pilot tube microtunneling originated in Japan and Europe, and was introduced to the United States in the year 1995 (Boschert 2007). Since then this methodology has seen increased utilization across North America particularity in municipal markets for the installation of gravity sewers. The primary reason contributing to the growth of pilot tube microtunneling is the technology's capability of installing pipes at high precision in terms of line and grade, in a wide range of ground conditions using relatively inexpensive equipment. The means and methods, applicability, capabilities and limitations of pilot tube microtunneling are well documented in published literature through many project specific case studies. However, there is little information on the macroscopic level regarding the technology and industry as a whole. With the increasing popularity of pilot tube microtunneling, there is an emerging need to address the above issues. This research effort surveyed 22 pilot tube microtunneling contractors across North America to determine the current industry state of practice with the technology. The survey examined various topics including contractor profile and experience; equipment, methods, and pipe materials utilized; and issues pertaining to project planning and construction risks associated with the pilot tube method. The findings of this research are based on a total of 450 projects completed with pilot tube microtunneling between 2006 and 2010. The respondents were diverse in terms of their experience with PTMT, ranging from two to 11 years. A majority of the respondents have traditionally provided services with other trenchless technologies. As revealed by the survey responses, PTMT projects grew by 110% between the years 2006 and 2010. It was found that almost 72% of the 450 PTMT projects completed between 2006 and 2010 by the respondents were for sanitary sewers. Installation in cobbles and boulders was rated as the highest risk by the contractors. / Dissertation/Thesis / M.S. Construction 2011

Orientador: Carola Dobrigkeit Chinellato / Dissertação (mestrado) - Universidade Estadual de Campinas, Instituto de Fisica Gleb Wataghin / Made available in DSpace on 2018-08-12T14:28:59Z (GMT). No. of bitstreams: 1 Pascon_AlineMaria_M.pdf: 7108044 bytes, checksum: c42a3aa2a12d613b0ce9e4e8da6014dc (MD5) Previous issue date: 2008 / Resumo: Foi aplicado o método de reconstrução de uma anisotropia de raios cósmicos para certo número de direções simuladas, a partir de uma distribuição consistindo de modulação dipolar sobreposta a um fluxo isotrópico. Usou-se o método proposto por J. Aublin e E. Parizot, que permite reconstruir a amplitude e orientção do dipolo no espaço 3D para um observatório que possui apenas uma visão parcial do céu, como é o caso do Observatório Pierre Auger Sul. Admitiu-se que a exposição do observatório para uma dada direção depende apenas da declinação, sendo independente da ascensão reta. A modulação dipolar foi considerada apontando na direção do centro galáctico e levou-se em conta o efeito do erro experimental na determinação da direção fazendo-a utuar segundo uma distribuição gaussiana de meia largura igual ao erro experimental do detector (1,5o). Estudou-se a capacidade de reconstrução deste dipolo, em direção e amplitude, para as condições do Observatório Pierre Auger, para vários valores de amplitude do dipolo e vários números de eventos, e foram obtidos os limites de tempo observacional necessários para detecção em cada caso, para um fluxo de eventos com energias acima de 10 18,5 eV . Por fim, calculou-se o número de eventos e o tempo necessário de observação para detectar uma anisotropia dipolar de 1%, para as condições do Observatório Pierre Auger, para atingir um valor de significância considerável, de 3, valor mínimo necessário para dar atenção à possível existência de anisotropia dipolar / Abstract: A method to study the anisotropy of a cosmic-ray distribution was applied to a number of directions simulated from an angular distribution of arrival directions consisting of a dipolar modulation of an otherwise isotropic ux. The method was suggested by J. Aublin and E. Parizot and allows reconstructing the amplitude and the dipole orientation in 3D space for a partial-sky coverage observatory like the Southern site of the Pierre Auger Observatory. The exposure of the observatory was assumed to be dependent only on the declination of the arrival directions and not on its right ascension. The dipolar modulation was taken to be pointing towards the galactic center. The experimental uncertainty in the direction measurements was also considered, represented by a smearing of all directions with a Gaussian uctuation with a half width corresponding to the experimental uncertainty (1,5 o). The capability of the Southern Pierre Auger Observatory of reconstructing the dipole modulation was studied for various dipole amplitudes and number of events. The observational time which is necessary for the detection was evaluated assuming that the observatory is fully efficient for energies above 10 18,5 eV . The number of events and corresponding observational time for detecting a 1% dipolar anisotropy with the Pierre Auger Observatory with a 3 s significance was also estimated / Mestrado / Teorias Especificas e Modelos de Interação ; Sistematica de Particulas : Raios Cosmicos / Mestre em Física

Abstract In this thesis, different spectroscopic methods are used for studying metals. Electron spectroscopy is applied for the study of binding energy shifts between atomic vapor and solid metals. Photoionization and Auger decay of high temperature aluminum vapors are investigated. Ionization of atomic chromium metal vapor by light absorption is studied with synchrotron radiation and time-of-flight ion mass spectroscopy. Optical spectroscopy is used for studying light emission from electric arc furnace plasma in experimental apparatuses developed during this work. Experimental techniques and sample preparation methods are presented.

Abstract The present thesis investigates the electronic structure of selected atoms and molecules in vapor phase. Electron spectroscopy is applied for studying the electronic transitions following excitation and ionization with electron and photon bombardment. The work focuses on the photoionization and Auger decay of selected noble gasses, and on the photoionization and Auger decay of core ionized or resonant excited alkali halide molecules. The experimental results are compared with theoretical predictions.

Abstract Electron-ion coincidence spectroscopy has been used to study the fragmentation behaviour of small molecules in two distinct cases: to resolve the state specific fragmentation patterns of HgBr₂ and HgCl₂ subsequent to UV ionization, and to distinguish the dissociation behaviour of various chlorinated methanes (CH₃Cl, CH₂Cl₂, CHCl₃ and CCl₄) subsequent to X-ray irradiation. The mercury-compound work has revealed details on the electronic structure and dissociation dynamics of the valence states which were previously unknown. The study on the chlorinated methanes has found new details on the exact pathways of the appearance of a specific fragment and also investigates the speed of the dissociation in the four different chlorinated methane molecules. The results from the electron-ion coincidence spectroscopic experiments are presented and analysed together with theoretical and computational support.

The direct growth of graphene by scalable methods on magnetic insulators is important for industrial development of graphene-based spintronic devices, and a route towards substrate-induced spin polarization in graphene without spin injection. X-ray photoelectron spectroscopy (XPS), low energy electron diffraction LEED, electron energy loss spectroscopy (EELS) and Auger electron spectroscopy (AES) demonstrate the growth of Co3O4(111) and CoO(111) to thicknesses greater than 100 Å on Ru(0001) surfaces, by molecular beam epitaxy (MBE). The results obtained show that the formation of the different cobalt oxide phases is O2 partial pressure dependent under same temperature and vacuum conditions and that the films are stoichiometric. Electrical I-V measurement of the Co3O4(111) show characteristic hysteresis indicative of resistive switching and thus suitable for advanced device applications. In addition, the growth of Co0.5Fe0.5O(111) was also achieved by MBE and these films were observed to be OH-stabilized. C MBE yielded azimuthally oriented few layer graphene on the OH-terminated CoO(111), Co0.5Fe0.5O(111) and Co3O4(111). AES confirms the growth of (111)-ordered sp2 C layers. EELS data demonstrate significant graphene-to-oxide charge transfer with Raman spectroscopy showing the formation of a graphene-oxide buffer layer, in excellent agreement with previous theoretical predictions. XPS data show the formation of C-O covalent bonding between the oxide layer and the first monolayer (ML) of C. LEED data reveal that the graphene overlayers on all substrates exhibit C3V. The reduction of graphene symmetry to C3V – correlated with C-O bond formation – enables spin-orbit coupling in graphene. Consequences may include a significant band gap and room temperature spin Hall effect – important for spintronic device applications. The results suggest a general pattern of graphene/graphene oxide growth and symmetry lowering for graphene formation on the (111) surfaces of rocksalt-structured oxides.