Made available in DSpace on 2016-04-25T20:21:25Z (GMT). No. of bitstreams: 1 Vivian Catarina Dias.pdf: 16086007 bytes, checksum: 6a5f546b854fa268d7eb63cefb940bd8 (MD5) Previous issue date: 2015-05-04 / Conselho Nacional de Desenvolvimento Científico e Tecnológico / Since childhood Charles Darwin showed an interest for the natural world. In his youth he developed that passion into an object of study travelling around the world onboard of The Beagle. The contact with unique species like the ones found in Galapagos Islands, the fossils discovered in South America, the experiences he lived and a extensive reseach during more than twenty years after his return to England made him think about a common link between the species, that lead him to publish The origin of species in 1859 and The expression of emotions in man and animals in 1872, along with other works and articles on the most diverse subjects, such as Diary of a naturalist around the world. In The origin of species he stated that the species are mutable and linked by the proccess of communion of ascendancy , fruits from the same tree of life. Forging the recreation of human history and its relocation in the natural world, the essays in The expression of emotions in man and animals widened the statement about the evolution of species pointing that humans share emotions and feelings with other species of animals. The implosion of the concept of human initiated with the darwinian facts opened new horizons for sciences such as anthropology to rethink the trajectory of the living beings and the borderlines between culture and animality. Notwithstanding, go beyond frontiers and enrich the critic, scientific, ethic and poetic repertoire is a really dificult task. Despite the relevance of the Darwinian Works, the contact with them is through indirect ways: cartoons, films and fiction, whose contents rarely approach the issue in all its depth / Desde a infância, Charles Darwin demonstrou interesse pelo mundo natural. Na juventude, transformou essa paixão em objeto de estudo viajando ao redor do mundo no navio Beagle. O contato com espécies únicas como as encontradas nas Ilhas Galápagos; os fósseis achados na América do Sul; as experiências adquiridas, e uma extensa pesquisa realizada por mais de vinte anos após o retorno à Inglaterra, levaram-no a pensar na filiação comum das espécies, desdobrando no lançamento de A origem das espécies (1859) e A expressão das emoções no homem e nos animais (1872), além de outras obras e artigos sobre os mais variados assuntos, como o Diário de um naturalista à volta do mundo. Em A origem das espécies, enunciou que as espécies são mutáveis e vinculadas pelo processo da comunhão de descendência , frutos da mesma árvore da vida. Forjando a recriação da história humana e sua realocação no mundo natural, os estudos de A expressão das emoções no homem e nos animais ampliaram o enunciado sobre a evolução das espécies, indicando que humanos compartilham emoções e sentimentos com outras espécies animais. A implosão do conceito de humano iniciada com os fatos darwinianos abriu outros horizontes para as ciências, incluindo a antropologia, repensarem a trajetória dos seres vivos e as fronteiras entre cultura e animalidade. Contudo, transpor limiares e enriquecer o repertório crítico, científico, ético, poético, mostra-se tarefa deveras espinhosa. Apesar da relevância das obras darwinianas, o contato com elas dá-se por vias indiretas: charges, filmes, ficção, cujos conteúdos, raramente, as abordam em todo seu alcance

Made available in DSpace on 2016-04-26T14:55:21Z (GMT). No. of bitstreams: 1 Vivian Catarina Dias.pdf: 16086007 bytes, checksum: 6a5f546b854fa268d7eb63cefb940bd8 (MD5) Previous issue date: 2015-05-04 / Conselho Nacional de Desenvolvimento Científico e Tecnológico / Since childhood Charles Darwin showed an interest for the natural world. In his youth he developed that passion into an object of study travelling around the world onboard of The Beagle. The contact with unique species like the ones found in Galapagos Islands, the fossils discovered in South America, the experiences he lived and a extensive reseach during more than twenty years after his return to England made him think about a common link between the species, that lead him to publish The origin of species in 1859 and The expression of emotions in man and animals in 1872, along with other works and articles on the most diverse subjects, such as Diary of a naturalist around the world. In The origin of species he stated that the species are mutable and linked by the proccess of communion of ascendancy , fruits from the same tree of life. Forging the recreation of human history and its relocation in the natural world, the essays in The expression of emotions in man and animals widened the statement about the evolution of species pointing that humans share emotions and feelings with other species of animals. The implosion of the concept of human initiated with the darwinian facts opened new horizons for sciences such as anthropology to rethink the trajectory of the living beings and the borderlines between culture and animality. Notwithstanding, go beyond frontiers and enrich the critic, scientific, ethic and poetic repertoire is a really dificult task. Despite the relevance of the Darwinian Works, the contact with them is through indirect ways: cartoons, films and fiction, whose contents rarely approach the issue in all its depth / Desde a infância, Charles Darwin demonstrou interesse pelo mundo natural. Na juventude, transformou essa paixão em objeto de estudo viajando ao redor do mundo no navio Beagle. O contato com espécies únicas como as encontradas nas Ilhas Galápagos; os fósseis achados na América do Sul; as experiências adquiridas, e uma extensa pesquisa realizada por mais de vinte anos após o retorno à Inglaterra, levaram-no a pensar na filiação comum das espécies, desdobrando no lançamento de A origem das espécies (1859) e A expressão das emoções no homem e nos animais (1872), além de outras obras e artigos sobre os mais variados assuntos, como o Diário de um naturalista à volta do mundo. Em A origem das espécies, enunciou que as espécies são mutáveis e vinculadas pelo processo da comunhão de descendência , frutos da mesma árvore da vida. Forjando a recriação da história humana e sua realocação no mundo natural, os estudos de A expressão das emoções no homem e nos animais ampliaram o enunciado sobre a evolução das espécies, indicando que humanos compartilham emoções e sentimentos com outras espécies animais. A implosão do conceito de humano iniciada com os fatos darwinianos abriu outros horizontes para as ciências, incluindo a antropologia, repensarem a trajetória dos seres vivos e as fronteiras entre cultura e animalidade. Contudo, transpor limiares e enriquecer o repertório crítico, científico, ético, poético, mostra-se tarefa deveras espinhosa. Apesar da relevância das obras darwinianas, o contato com elas dá-se por vias indiretas: charges, filmes, ficção, cujos conteúdos, raramente, as abordam em todo seu alcance

Le maintien des tissus péri-implantaires sains à un niveau le plus coronaire possible conditionne le succès à long terme des réhabilitations implanto-portées. Dès lors, la mise en place d'une barrière muqueuse protectrice et la préservation de l'os marginal au cours des premières semaines de cicatrisation est indispensable. Le design implantaire influence les remodelages tissulaires intervenant au niveau du col de l'implant lors des phases initiales de cicatrisation. L'objet de ce travail est d'analyser histologiquement chez le Beagle l'influence de deux systèmes implantaires innovants sur les caractéristiques de la muqueuse et la position de l'os marginal après 3 et 12 semaines de cicatrisation. Les résultats de cette étude, mis en relation avec les données de la littérature indiquent que les implants « deux-parts » présentant une connectique cône morse pourraient favoriser la mise en place d'un espace biologique court et limiter la perte osseuse marginale péri-implantaire. Les implants « une part » à col transmuqueux concave seraient quant à eux associés à des valeurs réduites d'espace biologique et autoriseraient une apposition osseuse sur l'épaule de l'implant. Dans la première partie de ce mémoire, nous établissons une synthèse des données histologiques actuelles concernant les caractéristiques et les processus de cicatrisation des tissus péri-implantaires, et l'influence de la conception implantaire sur les remodelages tissulaires marginaux intervenant au cours de la cicatrisation. Nous avons rédigé deux publications internationales que nous présentons en deuxième partie. Une réflexion analytique autour de ce travail est présentée dans la troisième partie / Aesthetic and functional long-term success of implant-supported rehabilitations relies on the preservation of peri-implant tissue levels in the most coronal position. Therefore, the achievement of an efficient protective mucosal seal, and the preservation of the peri-implant marginal bone during the first weeks of healing are essential to prevent long term implant failures. The characteristics of an implant transmucosal design (connections, platforms, surface properties) are related to biological width dimensions, marginal peri-implant bone levels, and the amount of inflammation within the peri-implant soft tissues. The aim of the research work presented in this manuscript was to investigate the effect of two innovative implant systems on peri-implant mucosa maturation, dimensions, collagen fiber organization, and marginal bone levels after 3 and 12 weeks of healing in the beagle dog. The results, compared with previous data, show that platform-switched twopiece implants exhibit reduced values of biological width and marginal bone loss, and that a concave transmucosal design in one-piece implants is associated with a short vertical value of biological width, and promote a mechanical interlocking of the implant body at the connective tissue and marginal bone levels. In the first part of this report, an overview of the current knowledge concerning the characteristics and healing patterns of peri-implant tissues, and the influence of implant design on the early peri-implant tissue remodeling is established. We have written two international publications which are presented in the second part. An analytical reflection about this work is presented in the third part

La vue est sans doute le plus important des sens. L'anatomie, l'histologie et la physiologie de l'œil normale chez les espèces de laboratoire adultes ont généralement été bien documentées. Cependant, les références décrivant les caractéristiques histomorphologiques du développement oculaire postnatal chez les animaux de laboratoire demeurent sporadiques et incomplètes. L'évaluation de tissus oculaires provenant d'animaux immatures peut être nécessaire lors d’études précliniques juvéniles de toxicité effectuées dans le but d’évaluer l'innocuité de médicaments destinés à la population pédiatrique. En effet, les données découlant d'études précliniques réalisées avec des animaux matures ne sont pas toujours jugées appropriées pour évaluer la toxicité d'un composé lorsqu'il est administré à des enfants. Cependant, la rareté des références histologiques sur le développement postnatal chez les animaux, ainsi que l'absence courante de témoins appariés selon l'âge pour les animaux sacrifiés de façon précoce lors d’études juvéniles, peuvent rendre difficile l'analyse des structures de l’œil en développement. Ainsi, l'objectif de cette thèse était de fournir des connaissances histologiques et immunohistochimiques (IHC) sur le développement oculaire postnatal, de la naissance au stade prépubère, chez le rat Sprague Dawley (SD), cochon domestique (DP), cochon miniature Göttingen (MP) et chien Beagle (BG). Les résultats de cette thèse ont démontré l'immaturité marquée de toutes les structures oculaires chez les rats SD et les chiens BG à la naissance et pendant la période postnatale. À la naissance, la rétine chez ces espèces altriciales était encore composée de la couche neuroblastique externe fœtale, et plusieurs étapes cruciales de la rétinogenèse, mises en évidence avec l’IHC, se sont produites lors des premières semaines de vie. D’autres évidences d'immaturité oculaire chez ces espèces incluaient la stratification de l’épithélium cornéen lors de l'ouverture des paupières et la présence de vestiges de la vascularisation hyaloïde. En revanche, les yeux des DP et MP, considérés comme une espèce précoce, étaient davantage développés à la naissance, néanmoins, d'importants changements de morphogenèse ont été observés lors de la période postnatale. Par exemple, la rétine du cochon néonatal présentait des photorécepteurs peu développés. Chez toutes les espèces examinées, la prolifération cellulaire et l'engagement des cellules dans le cycle cellulaire, mis en évidence avec Ki-67 et/ou PHH3, étaient prédominants dans la majorité des structures oculaires en développement. L'apoptose, démontrée avec l'IHC contre caspase-3 activé et/ou l'histochimie TUNEL, s’est avérée une caractéristique histologique clé à des âges précis de la rétinogenèse chez les rats SD et les chiens BG. Ce changement était aussi notable dans l'épithélium immature du cristallin du rat SD, ainsi que dans les vestiges hyaloïdes chez toutes les espèces. Enfin, des évidences d’activation non-apoptotique de caspase-3 ont été observées dans différents types cellulaires chez toutes les espèces. Les connaissances présentées dans cette thèse pourront servir de référence pour les pathologistes devant évaluer des structures oculaires en développement dans le cadre d'études précliniques de toxicité. Par ailleurs, les résultats de cette thèse ouvrent la voie pour des investigations plus approfondies sur le développement oculaire, particulièrement chez le chien et le cochon, qui pourront servir à des recherches futures en ophtalmologie pédiatrique. / Vison is arguably the most important of senses. The normal anatomy, histology, and physiology of the eye in mature laboratory species have generally been well documented, particularly in rodents. However, references addressing the histomorphological features of the postnatal ocular development in laboratory animals, notably in nonprimate large animal models, remain sporadic and incomplete. From a veterinary toxicologic pathology perspective, the evaluation of ocular tissues from immature animals may be needed during different types of preclinical juvenile animal toxicity studies conducted to assess the safety of xenobiotics on the pediatric population. Data from preclinical studies conducted in mature animals are often not deemed appropriate to evaluate the toxicity of a drug when administered to children, thus warranting the need to use juvenile animals. However, the paucity of histological references describing the postnatal development of laboratory animals, along with the common lack of age-matched controls when animals are unexpectedly sacrificed (or found death) early during juvenile studies, can render the analysis of developing ocular structures challenging. Thus, the objective of this thesis was to provide comprehensive histological and immunohistochemical (IHC) knowledge on the postnatal ocular development, using several age timepoints from birth to the peripubertal stage, in the Sprague Dawley (SD) rat, domestic pig (DP), Göttingen minipig (MP) and Beagle (BG) dog. Overall, the results from this thesis demonstrated the marked immaturity of all ocular structures in SD rats and BG dogs at birth and during the postnatal period. Notably, the retina at birth in these altricial species still contained the highly proliferative fetal outer neuroblastic layer, and critical retinogenesis events, highlighted with IHC, occurred rapidly during the first few weeks of life. Other noteworthy features of ocular immaturity in these species included the corneal epithelial stratification happening around the time of eyelid opening, the presence of hyaloid vascular remnants, and the globally poorly developed eye anterior segment. Contrastingly, the eyes of the DP and MP, considered a precocial species, were appreciably more developed at birth, although important ocular morphogenesis changes still occurred after birth. Importantly, the neonatal pig retina presented poorly developed cone and rod photoreceptors. In all examined species, cellular proliferation and the engagement of cells in the cell cycle, highlighted by Ki-67 and/or PHH3 IHC, were prominent in nearly all developing ocular structures for variable periods of time. Physiologically occurring apoptosis, highlighted by cleaved-caspase-3 IHC and/or TUNEL histochemistry, was a key histological feature of retinogenesis at specific age timepoints in SD rats and BG dogs, and was notable in the SD rat immature lens epithelium, as well as in regressing hyaloid vasculature remnants of all species. Lastly, evidence of nonapoptotic activation of caspase-3 was observed in different ocular cell types in all species. The information presented in this thesis will hopefully serve as general reference material for pathologists evaluating immature ocular structures in the context of preclinical toxicity studies. Moreover, the results pave the way for more in-depth investigations of specific ocular developmental events in nonprimate large animal models that may be useful for pediatric ophthalmology translational research.

O teste de potencial auditivo de tronco encefálico (PEATE) permite captar e registrar as atividades elétricas do sistema auditivo, desde a cóclea até o tronco encefálico. No Braisl ainda é pouco utilizado, e no Sul do país ainda não é realizado em animas.O objetivo deste trabalho foi identificar os valores absolutos de latência, interpicos, amplitudes, comparar as polaridades de compressão e rarefação e conhecer o limiar auditivo em onze cães hígidos da raça Beagle. Os cães passaram por exame físico e neurológico e após foram sedados e submetidos a otoscopia e ao exame de Potencial Auditivo de Tronco Encefálico (PEATE). Foram utilizados eletrodos de superfície e fones de inserção, o estimulo utilizado foi o clique com estimulação bilateral. O teste foi iniciado com a intensidade de 90 dB SPL com polaridade condensada. Realizava-se na sequência mais uma pesquisa em 90 dB SPL com polaridade rarefeita. Após, foi feito o exame com diminuição da intensidade de 10 dB SPL em 10 dB SPL, sendo todas as pesquisas feitas com polaridade condensada, para a pesquisa dos limiares auditivos. A interpretação do PEATE, foi realizada pela avaliação das ondas I, III e IV, pela identificação das latências absolutas e interpicos, pesquisa do limiar auditivo e comparação entre polaridades. Na latência da onda I com polaridade rarefeita em 90 dB SPL, os valores médios de latência absoluta da onda I foram significativamente maiores na orelha esquerda (p=0,037*). Dados relativos à latência absoluta da onda V evidenciou que na orelha direita a latência foi significativamente menor na intensidade de 70 dB SPL, e não houve presença de onda V na orelha esquerda em 40 dB SPL. Não houveram diferenças significativas nas latências dos interpicos entre as orelhas direita e esquerda, com polaridade condensada e rarefeita. Os valores das medianas das amplitudes I, III e V com 90 dB SPL em compressão e rarefação, não diferiram estatisticamente. Concluiu-se que o nível de resposta mínimo se encontrou entre 40 dB SPL e 80 dB SPL. / The Brainstem Auditory Evoked Potential (BAEP) capture and record the electrical activity of the auditory system, from the cochlea to the brainstem. The Brazil is still little used, and in the South of the country is not yet done in animals. The objective of this study was to identify the absolute values of latency, interpeaks, amplitudes, compare the polarities of compression and rarefaction and to know the auditory threshold in eleven healthy beagle dogs. The dogs underwent physical and neurological examination and were sedated and submitted to otoscopy and Brainstem Auditory Evoked Potential (BAEP). Surface electrodes and insertion headphones were used, the stimulus used was the click with bilateral stimulation. The test was started with 90 dB SPL intensity with condensed polarity. Further research was conducted on 90 dB SPL with rarefied polarity. After that, the test was performed with sequential reductions of 10 dB SPL intensity, and all the research for the auditory thresholds was done with condensed polarity. The interpretation of BAEP was performed by the evaluation of waves I, III and IV, by the identification of the absolute and interpeak latencies, auditory threshold research and comparison between polarities. In wave I latency with a rarefied polarity of 90 dB SPL, the mean absolute values of wave I latency were significantly higher in the left ear (p = 0.037*). Data on absolute V wave latency showed that, in the right ear, the latency was significantly lower in the intensity of 70 dB SPL, and there was no presence of V wave in the left ear in 40 dB SPL. There were no statistically significant differences in the interpeak latencies between the right and left ears, with condensed and rarefied polarity. The values of the medians of amplitudes I, III and V with 90 dB SPL in compression and rarefaction did not differ statistically. The minimum response level was found between 40 dB SPL and 80 dB SPL.

Some dairy breeds have too few animals genotyped for within breed genomic selection to be carried out with sufficient accuracy. As such, the level of linkage disequilibrium within each breed as well as consistency of gametic phase across breeds was studied. High correlations of phase (>0.9) were found between all breed pairs at this same SNP density. The efficacy of imputing animals genotyped on lower density (6k and 50k) panels was then explored in order to increase the size of the reference population with 777k genotypes in a cost-effective manner. These results showed high accuracies (>0.92) in all imputation scenarios studies, using both a within breed and a multi-breed reference population for imputation. It was concluded that given the results of both of these studies, pooling breeds into a common reference population for genomic selection should be a viable option for accurate genomic selection in breeds with few genotyped individuals. / NSERC, USDA, CDN, DairyGen, Ayrshire Canada, Guernsey Canada, Semex, L'Alliance Boviteq Inc.

O teste de potencial auditivo de tronco encefálico (PEATE) permite captar e registrar as atividades elétricas do sistema auditivo, desde a cóclea até o tronco encefálico. No Braisl ainda é pouco utilizado, e no Sul do país ainda não é realizado em animas.O objetivo deste trabalho foi identificar os valores absolutos de latência, interpicos, amplitudes, comparar as polaridades de compressão e rarefação e conhecer o limiar auditivo em onze cães hígidos da raça Beagle. Os cães passaram por exame físico e neurológico e após foram sedados e submetidos a otoscopia e ao exame de Potencial Auditivo de Tronco Encefálico (PEATE). Foram utilizados eletrodos de superfície e fones de inserção, o estimulo utilizado foi o clique com estimulação bilateral. O teste foi iniciado com a intensidade de 90 dB SPL com polaridade condensada. Realizava-se na sequência mais uma pesquisa em 90 dB SPL com polaridade rarefeita. Após, foi feito o exame com diminuição da intensidade de 10 dB SPL em 10 dB SPL, sendo todas as pesquisas feitas com polaridade condensada, para a pesquisa dos limiares auditivos. A interpretação do PEATE, foi realizada pela avaliação das ondas I, III e IV, pela identificação das latências absolutas e interpicos, pesquisa do limiar auditivo e comparação entre polaridades. Na latência da onda I com polaridade rarefeita em 90 dB SPL, os valores médios de latência absoluta da onda I foram significativamente maiores na orelha esquerda (p=0,037*). Dados relativos à latência absoluta da onda V evidenciou que na orelha direita a latência foi significativamente menor na intensidade de 70 dB SPL, e não houve presença de onda V na orelha esquerda em 40 dB SPL. Não houveram diferenças significativas nas latências dos interpicos entre as orelhas direita e esquerda, com polaridade condensada e rarefeita. Os valores das medianas das amplitudes I, III e V com 90 dB SPL em compressão e rarefação, não diferiram estatisticamente. Concluiu-se que o nível de resposta mínimo se encontrou entre 40 dB SPL e 80 dB SPL. / The Brainstem Auditory Evoked Potential (BAEP) capture and record the electrical activity of the auditory system, from the cochlea to the brainstem. The Brazil is still little used, and in the South of the country is not yet done in animals. The objective of this study was to identify the absolute values of latency, interpeaks, amplitudes, compare the polarities of compression and rarefaction and to know the auditory threshold in eleven healthy beagle dogs. The dogs underwent physical and neurological examination and were sedated and submitted to otoscopy and Brainstem Auditory Evoked Potential (BAEP). Surface electrodes and insertion headphones were used, the stimulus used was the click with bilateral stimulation. The test was started with 90 dB SPL intensity with condensed polarity. Further research was conducted on 90 dB SPL with rarefied polarity. After that, the test was performed with sequential reductions of 10 dB SPL intensity, and all the research for the auditory thresholds was done with condensed polarity. The interpretation of BAEP was performed by the evaluation of waves I, III and IV, by the identification of the absolute and interpeak latencies, auditory threshold research and comparison between polarities. In wave I latency with a rarefied polarity of 90 dB SPL, the mean absolute values of wave I latency were significantly higher in the left ear (p = 0.037*). Data on absolute V wave latency showed that, in the right ear, the latency was significantly lower in the intensity of 70 dB SPL, and there was no presence of V wave in the left ear in 40 dB SPL. There were no statistically significant differences in the interpeak latencies between the right and left ears, with condensed and rarefied polarity. The values of the medians of amplitudes I, III and V with 90 dB SPL in compression and rarefaction did not differ statistically. The minimum response level was found between 40 dB SPL and 80 dB SPL.

O teste de potencial auditivo de tronco encefálico (PEATE) permite captar e registrar as atividades elétricas do sistema auditivo, desde a cóclea até o tronco encefálico. No Braisl ainda é pouco utilizado, e no Sul do país ainda não é realizado em animas.O objetivo deste trabalho foi identificar os valores absolutos de latência, interpicos, amplitudes, comparar as polaridades de compressão e rarefação e conhecer o limiar auditivo em onze cães hígidos da raça Beagle. Os cães passaram por exame físico e neurológico e após foram sedados e submetidos a otoscopia e ao exame de Potencial Auditivo de Tronco Encefálico (PEATE). Foram utilizados eletrodos de superfície e fones de inserção, o estimulo utilizado foi o clique com estimulação bilateral. O teste foi iniciado com a intensidade de 90 dB SPL com polaridade condensada. Realizava-se na sequência mais uma pesquisa em 90 dB SPL com polaridade rarefeita. Após, foi feito o exame com diminuição da intensidade de 10 dB SPL em 10 dB SPL, sendo todas as pesquisas feitas com polaridade condensada, para a pesquisa dos limiares auditivos. A interpretação do PEATE, foi realizada pela avaliação das ondas I, III e IV, pela identificação das latências absolutas e interpicos, pesquisa do limiar auditivo e comparação entre polaridades. Na latência da onda I com polaridade rarefeita em 90 dB SPL, os valores médios de latência absoluta da onda I foram significativamente maiores na orelha esquerda (p=0,037*). Dados relativos à latência absoluta da onda V evidenciou que na orelha direita a latência foi significativamente menor na intensidade de 70 dB SPL, e não houve presença de onda V na orelha esquerda em 40 dB SPL. Não houveram diferenças significativas nas latências dos interpicos entre as orelhas direita e esquerda, com polaridade condensada e rarefeita. Os valores das medianas das amplitudes I, III e V com 90 dB SPL em compressão e rarefação, não diferiram estatisticamente. Concluiu-se que o nível de resposta mínimo se encontrou entre 40 dB SPL e 80 dB SPL. / The Brainstem Auditory Evoked Potential (BAEP) capture and record the electrical activity of the auditory system, from the cochlea to the brainstem. The Brazil is still little used, and in the South of the country is not yet done in animals. The objective of this study was to identify the absolute values of latency, interpeaks, amplitudes, compare the polarities of compression and rarefaction and to know the auditory threshold in eleven healthy beagle dogs. The dogs underwent physical and neurological examination and were sedated and submitted to otoscopy and Brainstem Auditory Evoked Potential (BAEP). Surface electrodes and insertion headphones were used, the stimulus used was the click with bilateral stimulation. The test was started with 90 dB SPL intensity with condensed polarity. Further research was conducted on 90 dB SPL with rarefied polarity. After that, the test was performed with sequential reductions of 10 dB SPL intensity, and all the research for the auditory thresholds was done with condensed polarity. The interpretation of BAEP was performed by the evaluation of waves I, III and IV, by the identification of the absolute and interpeak latencies, auditory threshold research and comparison between polarities. In wave I latency with a rarefied polarity of 90 dB SPL, the mean absolute values of wave I latency were significantly higher in the left ear (p = 0.037*). Data on absolute V wave latency showed that, in the right ear, the latency was significantly lower in the intensity of 70 dB SPL, and there was no presence of V wave in the left ear in 40 dB SPL. There were no statistically significant differences in the interpeak latencies between the right and left ears, with condensed and rarefied polarity. The values of the medians of amplitudes I, III and V with 90 dB SPL in compression and rarefaction did not differ statistically. The minimum response level was found between 40 dB SPL and 80 dB SPL.

The Martian atmosphere is of great scientific interest, both because of its similarity to Earth’s atmosphere, and because of its relevance to exploration of Mars. Although satellite instruments have provided a wealth of atmospheric data, they have provided little information about the atmospheric boundary layer. Conditions in the lowest few metres of the Martian atmosphere are perhaps the most directly interesting to humans, as this is the portion of our own atmosphere with which we have the most contact. In this thesis is described the design, calibration and operations planning for a new wind sensor for use on Mars. This sensor is lighter and smaller than previous Mars wind sensors. At the time of writing, the wind sensor is on its way to Mars as part of the science payload of Beagle 2, a small exobiology lander due to arrive in December 2003. The Beagle 2 wind sensor (B2WS) is a hot-film anemometer. Three platinum films are equally spaced around the surface of a vertical cylinder. A known current is dissipated in each film, heating the film 40-80°C above the ambient gas temperature. The film temperature is obtained by measuring its resistance. An effective heat transfer coefficient is then calculated for each film. A novel scheme has been developed which allows calculation of a wind vector from the differences between these heat transfer coefficients, rather than from their average. This makes the measured wind vector less prone to common-mode errors such as uncertainties in air temperature or sky temperature. The sensor was calibrated in a low density wind tunnel, optimised to provide stable winds of air or carbon dioxide at Martian pressures (5 – 10 mbar) and speeds (0.5 – 30 m/s). The flow field in the test section was calculated using analytical and finite element modelling techniques, and validated experimentally using a pitot probe. This facility’s stability and accuracy represent a significant improvement over previous calibration facilities. An analytical model of heat flow in the sensor has been developed in order to permit correction for conditions which may be encountered on Mars, but were not tested for in the wind tunnel. The wind sensor’s performance in a real Martian atmosphere is simulated using wind and temperature data from a previous Mars lander. The position of the wind sensor position at the end of Beagle 2's motorised arm allows several new possibilities for wind measurement on Mars that were unavailable in previous missions. The height of the wind and air temperature sensors can be adjusted to any height between 20 and 95 cm above the ground. The temperature sensor can be scanned horizontally and vertically above the lander to study convective updrafts above the heated lander. Planned operations sequences on Mars are discussed.

Speculative fiction allows an expanded view of literature and so allows scholars to explore new boundaries in the way words and ideas work. In the titular character of The Last Unicorn by Peter S. Beagle, the reader sees an expansion of self through liminality while A Scanner Darkly by Philip K. Dick explores its collapse. In order to portray each of these the character examined must move though one seems to move upward and the other downward. This idea of movement is only part of what expands the idea of liminality past the traditional idea of a doorway to create a hallway that the character might traverse on the way from place to place. This is not a redefinition of the term but a revision, a change in the way that we look at the concept as we accept and explore newer genres.