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Ettsyfte med denna uppsats är jämförelsen mellan två behandlingsalternativ för matavfall, hemkompostering eller biogasproducering sett från klimatperspektivet. Koldioxidekvivalenter har beräknats för de två alternativen. Ett annat syfte har varit att studera den politiska processen för att förstå varför det först under 90-talet uppmuntras till att hushållen ska hemkompostera för att sen under början av 2000-talet förespråka rötning av matavfallet. Mina uträkningar och jämförelser visar att det är mer fördelaktigt för klimatet att röta matavfallet, koldioxidutsläppet blir mindre än vid hemkompostering. Mitt andra syfte visar att det kan finnas en klimatvinst genom att låta politiken fatta besluten men det kan även finnas en risk att det egentliga syftet med hemkompostering glöms bort och att hushållen måste betala för något som de egentligen inte behöver (matkärl) eller som inte kanske finns (tillsynen). / One purpose of this study is to compare two treatment options for food waste. Home composting or biofuel production, seen from the climate perspective. The carbon dioxide equivalent has been calculated for the two options. The second purpose was to study the political process with regard to food waste to understand why Swedish households were encouraged to home compost food waste during the nineties only to early twenties advocate biofuel production. My calculations and comparisons shows that it is more beneficial for the climate to digest the food waste then composting since the carbon dioxide emissions is less. My second analysis indicates that while the political reorientation has led to environmental improvements, the effect has also been that to gain control of the food waste stream, waste management organisations effectively provide customers with no choice and economically punish households that have no food waste to collect since they have effective home composting.

Due to the increasing climate change concerns, biofuels have attracted more attention in the energy field as potential alternative energy sources. Particularly, microalgal biofuel has stood out because of its higher fuel yield potential and lower water and land demand than terrestrial biomass. Because of its outstanding photosynthetic efficiency, the microalgal technology is also investigated by researchers around the world as a potential biological solution for carbon capturing in the industrial sector. To explore the prospects of microalgal technology in a local context, this research lays it focus on investigating the potentiality of microalgal biofuel in the cement industry on Gotland, which is the largest emitter of greenhouse gases on the island. For this purpose, the thesis implements a series of estimations based on the emission data of Cementa AB, Slite, a picture of the potential production of algal biomass and biofuel was created, followed by comparisons to the energy situation on Gotland. While practical data of the selected microalgae species are presented, the results indicate a high potential of microalgae in the production of algal biofuel and the possibility for algal biofuel to power the industrial sector of Gotland, or even the island entirely. Although the estimations are made based on an assumption where all controlling parameters are assumed to be perfectly manipulated, the results still indicate the significance of microalgal technologies in the near-future bioeconomy and global energy system.

[pt] As emissões de combustíveis fósseis derivados do petróleo têm um impacto significativo no meio ambiente, nas mudanças climáticas, na poluição do ar e outros. Por isso, existe um grande interesse por fontes alternativas de energia, principalmente aquelas que podem reduzir as emissões de poluentes atmosféricos. Este trabalho teve por objetivo avaliar os perfis das concentrações de poluentes regulamentados (material particulado e CO2) e não regulamentados (benzeno, tolueno, etilbenzeno, xilenos, HPA, hidrocarbonetos alifáticos, aldeídos e metais) originados da queima de diferentes combustíveis em um motor estacionário do ciclo diesel e verificar os possíveis efeitos toxicológicos utilizando células epiteliais brônquica. Os combustíveis utilizados foram: mistura binária diesel com 5 por cento de biodiesel (B5), biodiesel de soja (B100), biodiesel de soja aditivado (B100 adt) e etanol aditivado. Para obter uma maior confiabilidade dos resultados realizou-se a verificação do método para a determinação de monoaromáticos e aldeídos por meio de cromatografia gasosa com detector de ionização de chama (CG-DIC) e cromatografia líquida de alta eficiência (CLAE), respectivamente. O MP foi determinado por gravimetria, Os HPA associados ao MP foram determinados por cromatografia gasosa acoplada a espectrometria de massa (CG-EM), hidrocarbonetos alifáticos (HA) por CG-DIC e metais foram analisados por espectrometria de massa com plasma indutivamente acoplado (ICP-MS). Os perfis das concentrações foram avaliados considerando: o tempo de queima de combustível (15, 30 e 60 min) e, o tempo de armazenamento (7, 14 e 21 dias) a 40 graus Celsius, para avaliar os processos oxidativos que podem ocorrer durante o armazenamento, especialmente no caso do biodiesel. A operação do motor quando ele ainda está frio, por exemplo, em 15 minutos, apresentou maior emissão de MP, benzeno e etilbenzeno para o biodiesel de soja (B100) em comparação com a mistura de diesel com 5 por cento de biodiesel (B5). O estudo dos perfis das concentrações dos compostos mono aromáticos, hidrocarbonetos alifáticos e hidrocarbonetos policíclicos aromáticos (HPA) mostraram resultados semelhantes aos descritos na literatura, em que o combustível B5 emiti mais poluentes do que o biodiesel. No entanto, comparando alguns poluentes individualmente, as emissões de benzeno e etilbenzeno foram maiores para B100 e B100 adt. O B5 apresentou o maior perfil de concentração para a soma HPA na fase gasosa do que na forma particulada em períodos de funcionamento do motor mais longos. O tempo de armazenamento reduziu as emissões de MP em 40 por cento para B100, 20 por cento para B100 adt e 3 por cento para B5. Em relação à concentração de compostos aromáticos, a redução foi de aproximadamente 60 por cento desses poluentes para B100 e B100 adt. A menor emissão de poluentes foi durante a queima do etanol aditivado. / [en] The emissions of fossil fuels derived from oil have a significant impact on the environment, climate change, air pollution, and others. Therefore, there is great interest in alternative energy sources, especially those that can reduce emissions of air pollutants. The aim of this study was the evaluation of concentration profiles of criteria pollutants, particulate matter (PM) and CO2 and unregulated pollutants (i.e., benzene, toluene, ethylbenzene, xylenes, polycyclic aromatic hydrocarbons (PAH), aliphatic hydrocarbons, aldehyde and metals) originated from burning of different fuels: The evaluated fuels were the binary mixture of fossil diesel with 5 percent biodiesel (B5), biodiesel soybean (B100), additivated biodiesel soybean (B100 adt) and additivated ethanol using a stationary diesel engine cycle, operating at the speed of 1800 rpm and 0 percent load. For a larger reliability of results, the method validation for determination the monoaromatic and aldehydes by gas chromatography flame ionization detection (GC-FID) and high-performance liquid chromatography (HPLC), respectively. PM mass was determinate by gravimetry, PAH associated to the PM were determined by gas chromatography coupled to mass spectrometry (GC-MS), aliphatic hydrocarbons were determined by GC-FID, and metals were determined by inductively coupled plasma mass spectrometry (ICP-MS). The concentration profiles were evaluated considering: 1) burning time (15, 30, and 60 minutes) and; 2) storage time (7, 14, and 21 days) at 40 Celsius Degrees to evaluate the oxidative processes that occur during storage, especially for biodiesel. When it is still cold (i.e., 15 minutes), the operation of the engine showed negative effect on particulate matter (PM), benzene, and ethylbenzene emissions for B100 compared to B5. In this study, the concentration profiles of the monoaromatic, aliphatic hydrocarbons, and PAH showed similar results to those reported in the literature, where B5 fuel emits more pollutants than pure biodiesel. However, comparing some pollutants individually, the benzene and ethylbenzene emissions were higher for B100 and B100 adt. For long engine operation periods, B5 showed the highest concentration profile for the PAH sum in the gaseous in comparison to PAH sum in the particulate phase. The storage time reduced the PM emissions in 40 percent (B100), 20 percent (B100 adt), and 3 percent (B5). Regarding the concentration of aromatics, the reduction was circa 60 percent for B100 and B100 adt. The lowest emission of pollutants was observed for additived ethanol burning.

Switchgrass is a C4 perennial grass that is currently being developed for use as a second generation lignocellulosic biofuel crop. For switchgrass to be fully utilized as a bioenergy crop, large-scale plantings of elite switchgrass germplasm, possibly in monoculture, are likely to occur. This practice may increase the selection pressure on plant pathogens, such as switchgrass rust, which could result in devastating disease epidemics. The identification and deployment of quantitative trait loci (QTLs) and major plant disease resistance genes (R) in switchgrass breeding programs could offer broad spectrum and durable disease resistance in commercial switchgrass cultivars. 'Alamo', a lowland cultivar, is generally resistant to switchgrass rust whereas 'Dacotah', an upland cultivar, is highly susceptible. I hypothesized that major R genes and/or QTLs were contributing to the differences in disease phenotypes of these two cultivars. In this dissertation, bioinformatics and molecular biology approaches were employed to dissect the genetic mechanisms underlying switchgrass rust disease resistance. Novel pseudo-F2 mapping populations were created from a cross derived from 'Alamo' and 'Dacotah'. RNA-sequencing of the pseudo-F2 progenies of 'Alamo' x 'Dacotah' was used to construct a genetic linkage map and to identify potential QTLs correlating with disease resistance. In addition, a homology-based computational method was used to identify 1,011 potential NB-LRR R genes in the switchgrass genome (v 1.1). These potential R genes were characterized for polymorphism and expression differences between 'Alamo' and 'Dacotah'. Moreover, I found that some NB-LRR genes are developmentally regulated in switchgrass. One of the major objectives of switchgrass breeding programs is to develop cultivars with improved feedstock quality; however, changes in the components of the plant cell wall may affect disease resistance. I hypothesized that genetically modified switchgrass plants with altered cell wall components will respond differently than the wild-type to switchgrass rust. Transgenic switchgrass plants overexpressing AtSHN3, a transcription factor with known functions in epicuticular wax accumulation and cell wall deposition, were created. I found that AtSHN3-overexpressing transgenic switchgrass lines were more susceptible than wild-type plants in their response to switchgrass rust. Overall, the results of this dissertation provide a platform for elucidating the molecular mechanisms underlying resistance of switchgrass to switchgrass rust. These findings will help breeders create switchgrass cultivars with improved disease resistance, and will ultimately allow switchgrass to be used for sustainable biomass production. / Ph. D.

Hydrogen production from abundant renewable biomass would decrease reliance on crude oils, achieve nearly zero net greenhouse gas emissions, create more jobs, and enhance national energy security. Cell-free synthetic pathway biotransformation (SyPaB) is the implementation of complicated chemical reaction by the in vitro assembly of numerous enzymes and coenzymes that microbes cannot do. One of the largest challenges is the high cost and instability of enzymes and cofactors. To overcome this obstacle, strong motivations have driven intensive efforts in discovering, engineering, and producing thermostable enzymes. In this project, ribose-5-phosphate isomerase (RpiB), one of the most important enzymes in the pentose phosphate pathway, was cloned from a thermophile Thermotoga maritima, and heterologously expressed in Escherichia coli, purified and characterized. High-purity RpiB was obtained by heat pretreatment through its optimization in buffer choice, buffer pH, as well as temperature and duration of pretreatment. This enzyme had the maximum activity at 80°C and pH 6.5-8.0. It had a half lifetime of 71 h at 60°C, resulting in its turn-over number of more than 2 x108 mol of product per mol of enzyme. Another two thermostable enzymes glucose-6-phosphate dehydrogenase (G6PDH) and diaphorase (DI) and their fusion proteins G6PDH-DI and DI-G6PDH were cloned from Geobacillus stearothermophilus, heterologouely expressed in E. coli and purified through its His-tag. The individual proteins G6PDH and DI have good thermostability and reactivity. However, the presence of DI in fusion proteins drastically decreased G6DPH activity. However, a mixture of G6PDH and a fusion protein G6PDH-DI not only restored G6PDH activity through the formation of heteromultimeric network but also facilitated substrate channeling between DI and G6PDH, especially at low enzyme concentrations. My researches would provide important building blocks for the on-going projects: high-yield hydrogen production through cell-free enzymatic pathways and electrical energy production through enzymatic fuel cells. / Master of Science

Currently extensive research on alternative fuels is being conducted due to their increasing demand to reduce greenhouse emissions. One renewable fuel studied in this work is dimethyl ether (DME) blended with propane(C3H8) as a potential mixture for heavy-duty engines used in semi-trucks. The blend has the potential to drastically reduce particulate and greenhouse gas emissions compared to a conventional diesel engine operating under similar conditions. To develop the use of mixture, one must conduct detailed conceptual and simulation studies before progressing to detail studies in CFD, engine modifications, and live testing. For simulations, accurate high-fidelity chemical kinetic models are necessary. However, the validity of the chemical kinetic mechanism for operating conditions of a heavy-duty mixing-controlled compression (MCCI) engine was widely unknown until recent work presented here and published. In this work, we studied the ignition of DME and propane blends in a shock tube under MCCI engine conditions. Ignition delay time (IDT) gathered behind the reflected shock for DME-propane mixtures for heavy-duty compression ignition (CI) engine parameters. Testing was conducted for undiluted varieties spanning from temperatures of 700 to 1100 K at pressures ranging from 55 to 84 bar for various blends (100% CH3OCH3, 100% C3H8, 60% CH3OCH3/ 40% C3H8) of DME and propane were combusted in synthetic air (21% O2/ 79% N2). Several experiments were conducted at higher pressures (90-120 bar) to improve the model performance and accuracy. The ignition delay times (IDTs) were compared to recent mechanisms, including Aramco3.0, NUIG, and Dames et al. A common trend among the mechanisms was overpredicted experimental IDTs. Further studies were conducted by a sensitivity analysis using the Dames et al. model, and critical reactions sensitive to IDTs of DME-propane mixture near 60 bar are outlined. Chemical analysis was conducted on the NTC region to explain chemical kinetics which is critical for developing MCCI heavy duty engines.

Thesis (MDF (Development Finance))--University of Stellenbosch, 2009. / ENGLISH ABSTRACT: Price volatility and high dependency on imported petroleum fuel has prompted the Zambian government to look into renewable fuels as part of an energy diversification program. With growing global interest in biofuels as a transportation fuel, the Zambian government intends to introduce bioethanol and biodiesel as renewable fuels in the transportation sector. While it seems feasible to produce both the feedstocks and biofuels to meet local demand, a regulatory framework and industry support mechanisms have not yet been formulated. The policy and regulatory frameworks encompass a multitude of actors, networks and institutions all playing distinct and important roles. Incorporating the differing interests of all these stakeholders is an involving process that requires detailed analysis of agriculture, environmental, energy, socioeconomic and taxation policies. This study attempts to contribute to the biofuels policy formulation process in Zambia. It analyses biofuel policies in leading biofuels producing countries and identifies aspects that the Zambian government should consider incorporating in its own policies to ensure a viable biofuels industry. Biofuel policies in Brazil, Germany and the United States of America were analysed using a detailed case study and extensive literature review. Furthermore, a detailed analysis of the Zambian agriculture sector and the demand for petroleum fuel puts into context the potential demand and challenges likely to be faced. By understanding the history and development of biofuels in the case study countries, best practices, problems faced, policy innovations and industry support mechanisms were identified to inform policy formulation in Zambia. This does not only provide valuable insights and lessons but also ensures that time and resources are not wasted by reinventing the wheel. The comparative analysis of policies and support mechanisms in the three case study countries showed that articulating a clear policy objective, government support in the form of subsidies, wide stakeholder involvement and industry regulation have all played a critical role in the development of the industry. However, the extent to which all these factors have helped to shape the industry in Brazil, Germany and the USA is neither equal nor static. Countries are continuously adapting their policies and support mechanisms to environmental, energy and economic conditions. / AFRIKAANSE OPSOMMING: Die onbestendigheid van pryse en die groot mate van afhanklikheid van ingevoerde petroleumbrandstof het die Zambiese regering aangespoor om ondersoek in te stel na hernubare brandstof as deel van 'n energiediversifiseringsprogram. In die lig van die groeiende globale belangstelling in biobrandstof as vervoerbrandstof, beplan die Zambiese regering om bioetanol en biodiesel as hernubare brandstof in die vervoersektor te begin gebruik. Al lyk dit prakties uitvoerbaar om sowel die voerstof as die biobrandstof te vervaardig om in die plaaslike aanvraag te voorsien, is 'n reguleringsraamwerk en ondersteuningsmeganismes vir die industrie nog nie geskep nie. 'n Menigte rolspelers, netwerke en instellings, wat almal verskillende en belangrike rolle speel, sal betrokke wees by die beleidsformulering en reguleringsraamwerk. Om die uiteenlopende belange van al die betrokke partye in ag te neem is 'n ingewikkelde proses wat sal vereis dat 'n uitvoerige analise gemaak word van landbou-, omgewings-, energie-, sosio-ekonomiese en belastingbeleidsrigtings. Die doelwit van hierdie studie is om 'n bydrae te lewer tot die formuleringsproses van die biobrandstofbeleid in Zambie. Dit analiseer die biobrandstofbeleid van die vooraanstaande lande wat biobrandstof vervaardig, en identifiseer aspekte wat die Zambiese regering in sy beleid behoort in te sluit om 'n lewensvatbare biobrandstofindustrie te verseker. Die biobrandstofbeleid van Brasilie, Duitsland en die Verenigde State van Amerika (VSA) is geanaliseer met behulp van uitvoerige gevallestudies en 'n grondige literatuurstudie. Verder plaas 'n noukeurige analise van die Zambiese landbousektor en die aanvraag na petroleumbrandstof die potensiele aanvraag en uitdagings wat waarskynlik hanteer sal meet word in konteks. Deur insig te verkry in die geskiedenis en ontwikkeling van biobrandstof in die lande waar die gevallestudies gedoen is, kon die beste gebruike, moontlike probleme, nuwe beleidsrigtings en ondersteuningsmeganismes in die bedryf geidentifiseer word om die beleid in Zambie te help formuleer. Dit bied nie slegs waardevolle insig en leergeleenthede nie, maar verseker ook dat tyd en hulpbronne nie vermors word deur die wiel van voor af uit te vind nie. Die vergelykende analise van die beleidsrigtings en ondersteuningsmeganismes in die drie lande waar die gevallestudies gedoen is, het getoon dat 'n duidelik geformuleerde beleidsdoelwit, ondersteuning van die regering in die vorm van subsidies, die algemene betrokkenheid van belanghebbendes en die regulering van die industrie alles 'n uiters belangrike rol gespeel het in die ontwikkeling van hierdie industrie. Die mate waarin al hierdie faktore die industrie in Brasilie, Duitsland en die VSA help vorm het, het egter gewissel en was nooit staties nie. Lande pas voortdurend hulle beleid en ondersteuningsmeganismes aan by omgewings-, energie- en ekonomiese toestande.