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Metodutprovning av ELISA detektion av anti-Porphyromonasgingivalis IgGDalstål, Elin January 2020 (has links)
No description available.
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En arytmijämförelse mellan unga elitidrottare och unga icke idrottande med 24h-Holter / An Arrhythmic Comparison between Young Elite Athletes and Young Non-athletes with 24h-HolterArnfjell, Emanuel January 2020 (has links)
No description available.
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The effects of urine adulteration with oxidants before drug analysis with LC-HRMS and CEDIANilsson, Anna January 2022 (has links)
In drug analysis, different kinds of immunoassays are often used as an initial screening for urine samples. The positive screening results are then followed by a confirmatory test, which consists of mass spectrometry methods to detect the drugs. The confirmatory tests are considered to be more resistant to manipulation than the screening tests. If the tested person wants to obtain a false negative result in the drug analysis, there are a lot of different manipulation strategies available today. Manipulation of urine with oxidants before the drug analysis, oxidizes the drugs, and obtains negative results. The aim of this study was to investigate how the results of the drug analysis with the screening test; cloned enzyme donor immunoassay (CEDIA) and the confirmatory test; liquid chromatography- high resolution mass spectrometry (LC-HRMS), were affected when the urine was manipulated with oxidants before the analysis. Four different oxidants (pyridinium chlorochromate, hypochlorite, sodium nitrite and povidone iodine) in three different concentrations, were investigated and evaluated on 37 different drugs with LC-HRMS. The most effective oxidant and the most adulterated drugs were then further investigated with LC-HRMS, CEDIA and with urine adulteration test strips. The results of the drug analysis were affected by the oxidants with both the CEDIA, and LC-HRMS analysis. All samples containing pyridinium chlorochromate and hypochlorite obtained negative results with CEDIA. With LC-HRMS, the tested samples containing 1 % hypochlorite effectively lowered the drug concentrations under the cut-off. Adulteration tests can be used to identify urine samples, manipulated with oxidants.
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Undersökning av latens, amplitud och nervledningshastighet i nervus peroneus communis / Study of latency, amplitude, and nerve conduction velocity in the common peroneal nerveBladh, Josefin January 2022 (has links)
Syftet med denna studie var att undersöka normalvärden för nervus peroneus communis (NPC) gällande latens, amplitud och nervledningshastighet samt undersöka om några signifikanta skillnader finns mellan höger respektive vänster NPC hos friska frivilliga deltagare mellan 20 och 40 år. Datainsamlingen utfördes på 25 deltagare på studentkliniken på Hälsohögskolan i Jönköping och maskinen Nicolet EDX® och programmet Synergy användes. Datainsamlingen skedde på tre stimuleringspunkter 1); 80 mm proximalt om musculus extensor digitorum brevis, 2); proximalt om caput fibulae och 3); 100–150 mm ovanför caput fibulae. Resultatet visade medelvärdet latens 4,49 ms, amplitud 10,5 mV och nervledningshastighet 58 ms gällande stimuleringspunkt 1. Latens 10,02 ms, amplitud 10,15 mV och nervledningshastighet 58 m/s för stimuleringspunkt 2. Latens 12,57 ms, amplitud 8,7 mV och nervledningshastighet 58,7 m/s för stimuleringspunkt 3. Icke-parametriska testet Mann-Whitney U Test visade en signifikant skillnad i latensen för stimuleringspunkt 1 gällande höger och vänster NPC p=0,04. Inga övriga variabler visade signifikant skillnad. Korrelationstest visade ett samband mellan längd och latens för stimuleringspunkt 1 och 2 p=0,011 respektive p=0,002. Ingen annan signifikant samband sågs mellan övriga variabler och längd. / The aim of the study was to investigate the normal value for the common peroneal nerve (NPC) in terms of latency, amplitude and nerve conduction velocity and investigate whether there are any significant differences between right and left NPC in healthy volunteers between 20 and 40 years of age. The data collection was performed on 25 participants at the student clinic at the School of Health and Welfare in Jönköping. The machine Nicolet EDX® and the program Synergy were used. Data collection took place at three stimulation points 1); 80mm proximal to the musculus (m.) extensor digitorium brevis, 2); proximal to the capute fibulae and 3); 100-150mm above the caput fibulae. The result showed the mean latency 4,49 ms, amplitude 10,5 mV and nerve conduction velocity 58 ms for stimulation point 1. Latency 10,02 ms, amplitude 10,15 mV and nerve conduction velocity 58 m/s for stimulation point 2. Latency 12,57 ms, amplitude 8,7 mV and nerve conduction velocity 58,7 m/s for stimulation point 3. The non-parametric test Mann-Whitney U Test showed a significant difference in latency for stimulation point 1 regarding right and left NPC p=0,04. No other variables showed a significant difference. Correlation test showed a relationship between length and latency for stimulation point 1 and 2, p=0,011 respectively p=0,002. No other significant correlation was seen between the other variables and height.
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Acidification of dog and cat urinary samples foranalysis of calcium and phosphorusSchönning, Angelica January 2022 (has links)
Introduction: Analysis of urine from dogs and cats are an important method for diagnosingkidney and urinary tract diseases. Earlier studies have suggested that urine needs to be acidifiedbefore analysis of calcium and phosphorus to obtain a more reliable result. The acidification ismeant to dissolve urinary crystals which could affect the analysis. Aim: The aim of this study was to investigate if acidification of dog and cat urinary samples isnecessary for reliable analysis of calcium and phosphorus. The study also aimed to investigate ifacidification is possible after the freezing of urine, and also evaluate the stability of acidifiedurine and supernatant in -80 °C freezer. Material and method: Forty-four urinary samples, of which 38 were from dogs and 6 from cats,were collected. Each sample was divided into three aliquots; one aliquot was acidified directly,one aliquot was acidified after storage in the freezer, and the last aliquot was centrifuged and thesupernatant was collected. Calcium and phosphorus were analyzed with an automatic chemistryinstrument in all aliquots before and after storage in -80 °C. The presence of urinary crystals wasinvestigated through a microscope. Results and conclusion: The calcium and phosphorus concentration were not higher in acidifiedurine, so acidification does not seem to be required for analysis of calcium and phosphorus indog and cat urine. Acidification after storage of urine in the freezer gave similar results asacidification of fresh urine. Both supernatant and acidified urine were stable in -80 °C for at least 3 days.
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Elektroneurografisk jämförelse av medianusnerven mellan frisörer och matchade kontroller : Förekomst av subklinisk karpaltunnelsyndrom hos frisörer?Lundmark, Lina January 2022 (has links)
No description available.
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Resistensbestämning av gramnegativa bakterier med VITEK 2 : Jämförelse mellan VITEK 2 och diskdiffusionFallström Mattsson, Johannes January 2022 (has links)
No description available.
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Hjärtfrekvensmätning vid isvaksbad - en metodjämförelse av två pulsmätningssystemMorén, Emma January 2022 (has links)
No description available.
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Analys med qPCR för bedömning av telomerlängdJohansson, Alva January 2022 (has links)
No description available.
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Analysis of lymphocyte subsets and intracytoplasmic cytokines in Covid-19 patients using flow cytometryVredin, Tuva January 2021 (has links)
Since late 2019 a new coronavirus, SARS-CoV2, has spread through the world and caused a pandemic. The virus causes the heterogenous disease Covid-19, causing flu- like symptoms for most people but can cause Acute Respiratory Distress Syndrome and fatal outcome in some patients. The mechanism is not clearly understood, but depletion of the immunological response has been seen in patients with severe Covid-19. This study will focus on T- lymphocyte, T- cell subtypes, B- lymphocyte, NK- cell and intracytoplasmic interleukin subsets in hospitalized Covid-19 patients to examine immune phenotypes that could be related to, or cause, more severe disease. The method used is multicolour flow cytometry on whole blood and frozen PBMC. Comparison of fraction of each cell type from total cell count, with regard of underlying diseases, age, sex and other factors were done using multivariate regression analysis to evaluate mechanisms correlated to severe disease. The results showed overall lower lymphocyte counts in Covid- patients versus healthy controls. Lower lymphocyte counts were also observed in men versus women. Lower levels of NK-cells and B-cells were found in people in ages over 60 years. Higher glucose levels were associated with lower B-cell and T-regulatory cell counts, while diabetes patients showed higher T-regulatory cell counts. No differences in proinflammatory cytokines were found between patient groups but were slightly higher in patients versus healthy controls. The conclusion of this study is that dysfunction of the immune system caused by infection of SARS-CoV2 gives lymphocytopenia and more severe outcome.
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