O vírus da influenza tipo A (VIA) é um importante agente em rebanhos suínos ao redor do mundo. Alguns subtipos do vírus podem ser transmitidos entre espécies diferentes, como aves, homem e suínos, proporcionando o aumento de mutações genômicas e de novas cepas circulantes. Os suínos são considerados hospedeiros de "mixagem", uma vez que possuem receptores para cepas de aves, de humanos e suínos. A doença clínica em suínos é caracterizada por quadro respiratório agudo e brando, com duração de 5 a 7 dias. Em rebanhos de reprodutores – como as Unidade Produtoras de Leitões (UPL) – um surto epidêmico de influenza pode levar o estabelecimento de uma infecção endêmica com duração de semanas a meses, sem produção de sinais clínicos evidentes. Protocolos de biosseguridade vêm sendo incorporados e padronizados pelas agroindústrias, visando prevenir a introdução de doenças infecciosas em rebanhos. Entretanto, existem lacunas no conhecimento dos fatores associados à biosseguridade em rebanhos de matrizes suínas brasileiras e sua relação com doenças infecciosas. Por essa razão, um estudo transversal foi realizado para estimar a soroprevalência do VIA em matrizes de UPL e explorar práticas de biosseguridade associadas à presença de anticorpos contra o vírus da influenza. Ao todo, foram amostradas 404 matrizes em 21 granjas. O diagnóstico sorológico foi realizado pelo ELISA (protocolo in house). Todas as amostras positivas pelo ELISA foram testadas usando a inibição de hemaglutinação (IH) para diagnosticar a presença de H1N1pdm2009, H1N2 e H3N2 como subtipos de vírus influenza. As informações sobre práticas de biosseguridade foram obtidas através da aplicação de um questionário. A associação entre o resultado do diagnóstico do ELISA de cada uma das matrizes amostradas e as práticas de biosseguridade da propriedade foi feitas através de um modelo de Regressão de Poisson Robusta, estimando a Razão de Prevalência (RP) como medida da associação. A prevalência estimada de anticorpos anti-VIA nas matrizes foi de 63,9% (IC 95%: 55% - 72%), sendo que todas as granjas tiveram resultados positivos. A frequência dos subtipos nas matrizes usando IH foi 51,9% para H1N1, 27,8% H1N2 e 0,6% H3N2. Coinfecções entre H1N1 e H1N2 foram observadas em 19 granjas. As práticas de biosseguridade associadas significativamente com a presença de anticorpos (p-valor <0,05) foram a "presença de tela anti-pássaros" (RP = 0,75) e "local de aclimatação para leitoas" (RP = 0,57) como fatores protetivos e "reposição externa de leitoas" (RP = 1,38) como associada a uma maior prevalência do vírus da influenza suína. Foi possível verificar que a soroprevalência do VIA nas matrizes comerciais da população estudada é alta, indicando que os animais são frequentemente expostos ao patógeno, e que algumas medidas de biosseguridade estão associadas com a ocorrência da doença, fornecendo subsídios técnicos sobre a importância dos protocolos de biosseguridade para a promoção da saúde do plantel. / Influenza A virus (IAV) is an important infectious agent in pig herds across the globe. Some subtypes of this virus can be transmitted between different species, such as birds, human and pig, increasing genomic mutations and evolving of new circulating strains. Pigs are considered "mixed vessel" for influenza A, since they have cell receptors for birds, humans and pigs strains. The clinical disease in pigs is characterized by an acute and mild respiratory disease, lasting from 5 to 7 days. In breeding herds such as sow farms, an epidemic outbreak of influenza can lead to the establishment of an endemic infection lasting weeks to months without clinical signs. Biosecurity procedures were incorporated and standardized by the agroindustry in order to prevent both the introduction and dissemination of infectious diseases. However, there are gaps in the knowledge about what biosecurity factors are associated with infectious diseases in Brazilian herds. For this reason, a cross-sectional study was carried out to estimate IAV seroprevalence in sows and assess which biosecurity practices are associated with the prevalence of influenza virus antibodies. Four hundred forty-four sows were sampled from 21 farms. Serological assays were performed using an ELISA test (in-house protocol). All ELISA positive samples were tested using the hemagglutination inhibition test (HI) to identify the presence of H1N1pdm2009, H1N2 and H3N2 subtypes. Information of biosecurity practices was obtained through the application of a questionnaire. Association between ELISA diagnostic result of each sampled sow and biosecurity practices was assessed using a robust Poisson regression and the Prevalence Ratio (PR) was used as the measure of association. The estimated prevalence of anti-IAV antibodies in sows was 63.9% (95% CI: 55% - 72%), and all farms had at least one seropositive sow s. The frequency of subtypes using HI was 51.9% for H1N1, 27.8% for H1N2 and 0.6% for H3N2. Co-infections with H1N1 and H1N2 were observed in 19 farms. Biosecurity practices such as "presence of bird-proof" (PR = 0.75), and "presence of an acclimatization unit" (PR = 0.57), protective ones, and "external replacement of gilts" (PR = 1.38), which was positively associated with the IAV prevalence, were statistically significant in the final model (p-value <0.05). It was possible to verify that IAV seroprevalence is high and some biosecurity procedures were associated with the serologic status, offering technical subsidies about the importance of the biosecurity for the herd heath.

Os procedimentos de limpeza e desinfecção usados na produção frangos de corte são fundamentais para a manutenção do alto nível de saúde do plantel, reduzindo o risco de ocorrência de enfermidades e melhorando o desempenho produtivo das aves. Existe a possibilidade de contaminação dos produtos desde a chegada das aves às instalações até o abate. Campylobacter é uma bactéria constantemente encontrada no trato gastrointestinal das aves e merece destaque na produção avícola devido a sua capacidade de provocar enfermidades em humanos. Para evitar a ocorrência de surtos de doenças transmitidas por alimentos como a campilobacteriose, para manutenção da saúde de plantéis avícolas e o aumento da produtividade de lotes de frangos de corte é necessário a realização das práticas preventivas como a limpeza e desinfecção. Desta maneira, o presente estudo teve como objetivos avaliar: o desempenho produtivo em lotes de frangos de corte desafiados com Campylobacter jejuni, a redução da pressão de infecção em instalações submetidas a diferentes protocolos de limpeza e desinfecção, e sua viabilidade econômico-financeira. Foi conduzido protocolo experimental no Laboratório de Pesquisa em Aves do departamento de Nutrição e Produção Animal, da Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo, Campus Pirassununga. Foram realizados dois alojamentos cada um com 960 pintos de um dia de idade. No primeiro alojamento, todas as aves foram inoculadas com uma cepa conhecida de Campylobacter jejuni (atcc 33560) a fim de contaminar o ambiente criando desafio sanitário. No segundo alojamento foram realizados dois protocolos de limpeza e desinfecção, constituindo os dois tratamentos experimentais (Comum e Proposto). Neste lote, para avaliação da produtividade, foram mensurados os parâmetros: viabilidade, mortalidade, conversão alimentar, ganho de peso e consumo de ração. Para avaliação microbiológica do ambiente e das carcaças foi realizada a contagem total de microrganismos para ambiente e a identificação de Campylobacter spp.. As amostras positivas foram submetidas a um ensaio de PCR multiplex para identificação de espécies de Campylobacter spp. Para avaliação econômica financeira dos lotes de frangos de corte foram utilizados e adaptados os dados de custos de produção elaborados pela Embrapa Suínos e Aves às condições de criação e resultados obtidos no presente estudo. O tratamento Proposto demonstrou influências positivas no desempenho das aves, na redução da contagem total de microrganismos, na eliminação de Campylobacter spp. e viabilidade econômico-financeira / The procedures of cleaning and disinfection used in broiler production are necessary to maintaining a high standard of birds health, decreasing the risk of diseases, optimizing the animal performance, as well as to ensure sanitary quality of meat. There is possibility of contamination in all farming steps, since chicks arrival until the end of slaughter. Campylobacter is a bacteria constantly found at gastrointestinal tract of the birds, and so, a special concern must be given since it can cause human diseases. In order to avoid foodborne illness outbreaks such as campylobacteriosis, to ensure poultry flocks health maintenance and to improve broilers productivity, adopting preventive practices such as cleaning and disinfection is required. Thus, this study aimed to evaluate: the animal performance of broilers challenged with Campylobacter jejuni; the reduction of infection pressure in housing plants subjected to different cleaning protocols and disinfection, and its economic and financial viability. Experimental protocol was conducted at the Research Laboratory of Poultry of Nutrition and Animal Production Department, College of Veterinary Medicine and Animal Science, University of São Paulo, Campus Pirassununga. Two housings each with 960 day-old chicks of age were performed. In the first housing, all birds were inoculated with a known strain of Campylobacter jejuni (ATCC 33560) to contaminate the environment in order to create health challenge. In the second housing were performed two cleaning and disinfection protocols, featuring two experimental treatments (Common and Proposed). In this lot, to evaluate animal performance, the parameters were measured: feasibility, mortality, feed conversion, weight gain and feed intake. For microbiological evaluation of environment and of carcasses, was performed a total count of microorganisms for environmental and identification of Campylobacter spp. The positive samples were subjected to a multiplex PCR assay identification of species of Campylobacter spp. For economic and financial evaluation of batches, the data of husbandry costs elaborated by Embrapa Swine and Poultry were adapted to conditions and rsults obtained in this study. Based on results, the Proposed treatment has shown positive influences on bird performance, reduction of total count of microorganisms, on elimination of Campylobacter spp. and on economic and financial viability

Since the first detected cases of Middle East Respiratory Syndrome Coronavirus (MERS-CoV), a respiratory virus capable of causing severe human illness, surveillance and research have focused on identification of the source of primary human MERS-CoV infections. Within the One Health framework which recognizes the interconnectedness of human, animal, and environmental health, various animal hosts were investigated as sources of human infection because most emerging infectious diseases are zoonoses – pathogens spilling over from an animal host to a human. A systematic literature review was conducted to synthesize knowledge about MERS-CoV infections in livestock animals and zoonotic transmission of MERS-CoV to humans. Dromedary camels are the presumptive source of primary human MERS-CoV infections, but the importance, frequency, and specific mechanisms of camel-to-human MERS-CoV transmission have not been well characterized. Further, it is unknown what potential barriers will be faced when implementing basic biosecurity interventions to prevent zoonotic transmission at occupational sites where individuals regularly work or interact with dromedary camels. This dissertation addressed these knowledge gaps through a study of MERS-CoV serology and knowledge, attitudes, and beliefs about camel-to-human disease risks and basic biosecurity interventions in Jordan. This study detected individuals with low MERS-CoV neutralizing antibody titers in Jordan and found an association between regular work or interaction with dromedary camels or their living environments and evidence of MERS-CoV infection. Specific behavioral practices were not associated with evidence of MERS-CoV infection among those who regularly work or interact with dromedary camels or their living environments in Jordan. The study also identified multiple important barriers that may be faced when implementing basic biosecurity interventions in this population, including personal protective equipment (PPE) use and frequent handwashing. There were widespread ingrained beliefs that dromedary camels cannot transmit disease to humans and that camel milk and camel urine are beneficial to human health. Among camel workers, reported PPE use was low and frequent handwashing was uncommon. Only a few camel workers felt they would use PPE even if made freely available at their place of work. In conclusion, this dissertation provides further evidence that dromedary camel exposure is an important pathway in the epidemiology of primary human MERS-CoV infections in Jordan. The findings also support the notion that an intervention that simply provides basic biosecurity materials such as PPE and handwashing stations is likely to be ineffective in this population, and that identified barriers should be addressed to motivate behavioral change. Future studies are proposed including a longitudinal cohort study of individuals at risk for MERS-CoV that can detect individual seroconversion over time, track antibody responses among those who seroconvert, and evaluate key sub-exposures among those who are occupationally exposed to dromedary camels; as well as a pilot study to evaluate the feasibility, acceptability, and sustainability of a multifaceted biosecurity intervention that provides materials as well as training and education targeting the knowledge, attitude, and belief barriers identified.

Submitted by Jorge Silva (jorgelmsilva@ufrrj.br) on 2016-10-25T18:01:56Z No. of bitstreams: 1 2005 - Eliane Mendon?a dos Santos.pdf: 652395 bytes, checksum: bd5ff58751aa2707364a743183c317ac (MD5) / Made available in DSpace on 2016-10-25T18:01:56Z (GMT). No. of bitstreams: 1 2005 - Eliane Mendon?a dos Santos.pdf: 652395 bytes, checksum: bd5ff58751aa2707364a743183c317ac (MD5) Previous issue date: 2005-12-13 / This paper has been done in Serop?dica, at Col?gio T?cnico da Universidade Rural (CTUR) and in Pinheiral, at Col?gio Agr?cola Nilo Pe?anha (CANP), both in Rio de Janeiro State, in the year of 2004. Two surveys have been given to the students at CTUR. Some teachers at CTUR answered questions about more contextualized learning, and the incentive to interaction among subjects. It was aimed to check the students perception as far as connections between the theme and the curriculum is concerned the relations with their daily activities. After a speech and na interative activity the teachers answered some more questions. At CANP, the course of farming is traditional. The students at CANP answered the questions of the first questionnaire. The hightschoolers demonstrated less envolviment with the subject. The technical students presented varied perceptions related to the theme. The answers from the students of the high school showed that they did not understand the matter goes through many subjects, thus a transversal theme. The professional course students were able to list a bigger number of subjects that have connections with the GMO. Most students classified the transgenic insufficiently. There have been many students interested in participating in the speech, as it was clear to note the need for more clarification about this issue so polemic and forward. / Este trabalho foi realizado em Serop?dica - RJ, no Col?gio T?cnico da Universidade Rural (CTUR) e em Pinheiral - RJ. no Col?gio Agr?cola Nilo Pe?anha (CANP) em 2004. Foram aplicados dois question?rios aos alunos do CTUR. Alguns docentes do CTUR responderam a perguntas, almejando uma aprendizagem mais contextualizada, e o est?mulo ? interdisciplinaridade. Buscou-se verificar a percep??o dos alunos quanto ?s liga??es e intera??es entre o tema e a grade curricular e as correla??es com o cotidiano. Ap?s a palestra e a atividade interdisciplinar, os docentes responderam a mais algumas perguntas. No CANP, o curso de Agropecu?ria ? tradicional. Os alunos do CANP responderam as quest?es do primeiro question?rio. Os alunos do Ensino M?dio demonstraram menos envolvimento com o assunto. Os discentes do t?cnico apresentaram percep??es variadas quanto ao tema. No Ensino M?dio, as respostas denotaram que eles n?o discerniram que a quest?o perneia v?rias disciplinas, pois ? um tema transversal. Os alunos do Ensino T?cnico elencaram um n?mero maior de disciplinas que possuem conex?es com os OGMs. A maioria dos alunos conceituou os transg?nicos de uma forma n?o satisfat?ria. Houve muitos alunos interessados em participar da palestra, ficou evidente o anseio por maiores esclarecimentos sobre este tema t?o pol?mico e atual.

The effectiveness of biosecurity measures at national borders is influenced by the behaviour of individuals. One influence on the behaviour of individuals is level of involvement. Involvement is the importance or relevance of an object or situation to an individual. Involvement helps regulate the way in which people receive and process information and thus influences the extent of information searching for decision making, information processing and persuasion. A second area of influence on individuals is the way in which information is framed. Framing influences the persuasiveness of communications. In this study the concept of involvement was used to investigate the response of individuals to New Zealand biosecurity requirements and the response of individuals to differently framed biosecurity information. A range of people associated with the agricultural and food processing sectors were surveyed using a five item scale of involvement to measure their level of involvement in biosecurity. The results indicated that most respondents had medium to high levels of involvement. This implies that respondents were motivated to attend to and process information on biosecurity measures. However, not all respondents reported taking note of biosecurity information implying that involvement with biosecurity prompts some initial information processing which may or may not continue over time. Respondents were also asked to rate four postcards, each designed with a different message strategy. Individuals with high involvement indicated that the postcard with the negatively framed emotion/entertainment message strategy was most persuasive. The results indicate that specifically targeting information to individuals based on their level of involvement in biosecurity may increase compliance with biosecurity measures.

This thesis is an investigation of external biosecurity and its application to risk-based surveillance approaches in the southern Ontario swine industry. In each of two datasets, the best number of groups to describe biosecurity practices was identified, resulting in two groups with high biosecurity standards and one group with low biosecurity standards. Multinomial logistic regression models identified herd density, herd size, and herd type among significant predictors of biosecurity group membership. A map of southern Ontario that can be used as a tool in the risk-based surveillance of contagious swine diseases was developed using geographic information about swine density, and the distribution of herds belonging to the high biosecurity groups. Finally, multiple correspondence analysis examined how individual biosecurity practices form strategies on sow farms. Some practices that are generally considered high-risk were closely associated with other practices that mitigate the risk, suggesting that evaluation of the overall strategy is essential for complete assessment of biosecurity. / The Ontario Ministry of Agriculture, Food and Rural Affairs (under the Emergency Management research theme); Ontario Pork; the Ontario Pork Industry Council's Swine Health Advisory Board; the Natural Sciences and Engineering Research Council of Canada;

Protection of the agriculture and food infrastructure, defined as "the physical production and distribution systems critical to supporting national security and economic well-being, including all activities essential to food, feed, and fiber production, including all techniques for raising and processing livestock," is the responsibility of all stakeholders in the food supply chain (Spellman, 2008, p.8). Though many stakeholders emerge in the chain, producers are the primary line of defense to a disease epidemic. Many factors influence livestock producers‘ protective action decision process in relation to biological hazards. By identifying these factors in a specific producer population, more effective preparedness programs and messages can be developed by risk communicators. The purpose and objectives of this study determined Texas and Southwestern Cattle Raisers Association (TSCRA) members‘ perceptions of vulnerability to the Texas cattle industry, perceived emergency preparedness level, barriers to adoption of protective actions, and sources of animal health information. This study targeted 7,661 members of the TSCRA. An online survey questionnaire developed from previous research with similar populations allowed TSCRA members to respond to questions related to the objective of this study. A representative sample of TSCRA members from Texas, Oklahoma, and New Mexico responded to the survey. Recognizing susceptibility to biological hazards, TSCRA members identified high levels of perceived trust and reliability in local veterinarians as a source of information, consistent with previous studies. Although the majority of members reported they did not have a biosecurity plan implemented on their operations, they did recognize the necessity of preventative practices. TSCRA members were neutral on all barriers to adoption of protective actions; however, the barrier "lack of information" was rated higher by means as a barrier to adoption of protective actions. When investigating differences among noncommercial and commercial operations managed by TSCRA members, no statistical differences were identified in this study. However, when investigating differences among TSCRA members and number of cattle managed, a weak positive correlation was identified for perception of hazard by threat in relation to more animals managed. Findings of this study confirmed sources stating that the agriculture and food infrastructure is vulnerable to biological hazards as perceived by livestock owners (Motes, 2007; Spellman, 2008). Ashlock (2006) and Riley (2007) found similar perspectives of vulnerability among Oklahoma and Kansas producers further strengthening the need to protect the agriculture and food critical infrastructure as outlined by Homeland Security Presidential Directive – 9 (HSPD-9, 2004). Since it was determined that veterinarians are perceived to be the most reliable and trustworthy source of information by TSCRA members, local opinion leaders, such as veterinarians, should engage in train-the-trainer programs to ensure a consistent risk communication message is being disseminated.

This study had as objective to present and to discuss the methodologic and computational structure in the collection and analyzes of the data flow and information used in georeferencing system of the poultry of Rio Grande do Sul State. It was focused the field applicability, introducing the technologies of geoinformation in the management of poultry companies with the function of to georeference, to store, to monitor and to analyze data and information collected by the companies, as well as to use specific tools of geoprocessing which modernize these poultry companies and assist the management agencies in taking of decisions, allowing them a higher security and rapidity concerning to biosecurity. The gotten results had allowed verifying that the production of georeferenced information regarding to poultry biosecurity is of vital importance for the economic and social situation of Brazil, therefore with them we can project action zones in a short interval of time from a focus, using a determined research radius, being able to take necessary prophylactic measures. It was concluded that the use of geoinformation technology for the production of georeferenced information reached the proposed objectives and revealed efficacious regarding to the planning, management and taking of decision. / Este estudo teve como objetivo apresentar e discutir a estrutura metodológica e computacional empregada na coleta e análise do fluxo de dados e informações utilizados no sistema de georreferenciamento da avicultura do Rio Grande do Sul referente à aplicabilidade de campo, introduzindo na gestão de empresas avícolas, as tecnologias de geoinformação com a função de georreferenciar, armazenar, monitorar e analisar dados e informações coletadas pelas empresas, bem como utilizar ferramentas especÍficas de geoprocessamento que modernizem estas empresas avícolas e auxiliem os órgãos na tomada de decisões permitindo a estas uma maior segurança e rapidez no que diz respeito à biossegurança. Os resultados obtidos permitiram verificar que a produção de informações georreferenciadas em relação à biossegurança avícola é de vital importância para a situação econômica e social brasileira, pois com as mesmas podemos em um curto intervalo de tempo projetar zonas de ações a partir de um foco utilizando um determinado raio de pesquisa, podendo com isto tomar as medidas profiláticas necessárias. A conclusão deste estudo é que o uso de tecnologia da geoinformação para a produção de informações georreferenciadas alcançou os objetivos propostos e mostrou-se eficaz no que tange ao planejamento, gestão e tomada de decisão.

This thesis addresses the role of education to mitigate the risks of deliberate disease, including biological weapons. Specifically, it aims to analyse how education was constructed as a potential instrument to mitigate specific security risks; if and how education could impact on risks; and how effectiveness of education as a risk mitigation measure could be improved. The research framework combines concepts of security, risk and education within a general constructionist approach. Securitization is used to analyse attempts to construct education as a tool to mitigate specific security risks; risk assessment is used to identify and characterize risk scenarios and potential for risks mitigation; and instructional design and evaluation models are used for the design and evaluation of education. The thesis contends that education has been constructed as a mitigation tool for what were presented as urgent security risks of deliberate disease. Nine attempted securitization moves are identified and assessed. Improved competences identified in four thematic areas, and built with education, can mitigate risks in specific scenarios via impacting factors that primarily influence risk likelihood. The thesis presents several examples of achieved learning objectives, and tools that can be useful to evaluate behavioural and risk impacts, though empirical results on these levels here are still scarce. Design and evaluation tools, illustrated through a large amount of original and pre-existing data from a range of countries and contexts, are presented that can improve effectiveness of education as a deliberate disease risks mitigation measure.

Orientador : Valéria Nogueira Dias Paes Secco / Banca: Valéria Nogueira Dias Paes Secco / Banca: Gilberto Luppi dos Anjos / Banca: Eliana Aparecida Sanches Tonolli / Resumo: Com o objetivo de analisar a prevalência da imunização contra a hepatite B entre os servidores municipais que atuam em ambiente de risco de transmissão ocupacional na Secretaria Municipal de Saúde (SMS) de Maringá, foi utilizado um questionário semi-estruturado autoexplicativo, com questões pertinentes à situação do esquema de vacinação e ao conhecimento e aplicação das condutas de biossegurança pelos servidores da área da saúde, em seu ambiente de trabalho. Participaram desta pesquisa 152 servidores das equipes de enfermagem, odontologia, análises clínicas e médica. O software SPSS (Statistical Package for the Social Sciences versão 12.0) foi utilizado para análise estatística. Quanto aos resultados obtidos, verificou-se que 99,3% dos servidores da amostra receberam a vacina contra a hepatite B, o que é considerado um resultado significativo, visto que o profissional de saúde faz parte do grupo de risco e o PNI Programa Nacional de Imunização do Ministério da Saúde recomenda a vacinação de todos que compõem esse grupo. Verificamos que 63,2% dos servidores desenvolvem suas atividades profissionais somente na Secretaria Municipal de Saúde (SMS) de Maringá. Dentre os profissionais com atividades de risco de transmissão ocupacional desenvolvidas fora da SMS de Maringá observamos que 15,1% desses servidores pertencem à equipe médica, 13,8% à equipe de enfermagem, 7,2% à equipe de odontologia e somente um profissional (0,7%) à equipe de análises clínicas. Em relação à orientação recebida para se vacinar contra a hepatite B, 93,4% dos servidores referiram tê-la recebido. A maioria dos servidores (98,7%) relataram ter conhecimento das medidas de prevenção contra risco de transmissão ocupacional e 92,8% dos servidores identificam risco de contato com sangue durante o desenvolvimento de suas atividades profissionais... (Resumo completo, clicar acesso eletrônico abaixo) / Abstract: This study aimed to analyzing the prevalence of hepatitis B immunization of among the city workers that work in environments with risk for occupational transmission in the City Health Care Secretary (CHCS) of Maringá. So, It was used a self-explaining and semi-structured questionnaire, with questions related to the situation of vaccination system and to the knowledge and application of biosecurity attitudes by health field professionals, in their workplace. Workers, in an amount of 152 individuals, took part in this survey and they were from nursing, odontology, clinic and medical analysis teams. The SPSS (Statistical Package for the Social Sciences) 12.0- version software was used for the statistical analysis. Concerning the obtained results, it was possible to verify that 99.3% of the sample workers received the hepatitis B vaccine, what is considered a meaningful result, since the health professional is part of the risk group and the NIP- National Immunization Program of the Health Ministry recommends the vaccination of every member from such group. We could verify that 63.2% of the workers perform their professional activities only in the City Health Care Secretary (CHCS) of Maringá. We could also observe that among the professionals with activities of risk for occupational transmission accomplished out of the CHCS of Maringá, 15.1% of these employees belong to the medical team, 13.8% are from the nursing one, 7.2% from the odontology group and only one professional (0.7%) is from the clinic analysis team. Concerning the received orientation to be vaccinated against hepatitis B, 93.4% of the employees said they had received it. Most of them (98.7%) said they knew the prevention measures against risk for occupational transmission and 92.8% of the workers identified contact risk with blood during the performance of their professional activities... ( Complete abstract, click eletronic address below) / Mestre