Made available in DSpace on 2014-06-11T19:28:23Z (GMT). No. of bitstreams: 0 Previous issue date: 2009-01-23Bitstream added on 2014-06-13T20:48:08Z : No. of bitstreams: 1 souza_lfa_me_jabo.pdf: 291371 bytes, checksum: 2921d3b2418a9b75c8fccd1974605cd7 (MD5) / Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) / Este trabalho avaliou o efeito da exposição ao calor crônico e cíclico sobre o desempenho, metabolização dos nutrientes, energia metabolizável aparente corrigida para balanço de nitrogênio (EMAn) e atividade de enzimas pancreáticas em frangos de corte. Foram utilizados 450 frangos, distribuídos em delineamento inteiramente casualizado com 5 esquemas de temperatura/alimentação (TN/Ad libitum, Crônico/Ad libitum, TN/restrito a Crônico, Cíclico/Ad libitum e TN/restrito a Cíclico). Assim, TN representa a temperatura termoneutra e os grupos TN/restrito a Crônico e TN/restrito a Cíclico receberam a mesma quantidade de ração que Crônico/Ad libitum e Cíclico/Ad libitum, respectivamente. A exposição crônica ao calor afetou o desempenho de frangos de corte com maior intensidade que a exposição cíclica, havendo efeito direto do calor crônico e cíclico. Os coeficientes de metabolização da matéria seca, gordura e a EMAn aumentaram com a idade. Os coeficientes de metabolização dos nutrientes foram diminuídos pela exposição crônica ao calor, havendo efeito direto da temperatura apenas para gordura. A restrição alimentar gerada pelo calor crônico causou aumento da atividade da amilase e redução na atividade da tripsina. A atividade da lipase aumentou devido ao efeito direto do calor crônico. A menor digestibilidade dos nutrientes foi associada à piora do desempenho das aves expostas ao calor crônico, porém não foi relacionada à piora dos índices zootécnicos dos frangos expostos ao calor cíclico. A redução da atividade da tripsina pode estar associada à menor digestibilidade da proteína causada pelo estresse por calor crônico. / This work evaluated the effect of the chronic and cyclical heat stress in performance, metabolization of nutrients, apparent metabolizable energy corrected for nitrogen balance (EMAn) and pancreatic enzymes activity in broiler chickens. 450 chickens, distributed in completely randomized design with 5 groups of temperature/feeding (TN/Ad libitum, Chronic/Ad libitum, TN/Chronic restrict, Cyclical/Ad libitum and TN/Cyclical restrict). Thus, TN represents the thermoneutral temperature and the TN/Chronic restrict and TN/Cyclical restrict had received the same amount from feed that Chronic/Ad libitum and Cyclical/Ad libitum, respectively. The chronic exposition to the heat affected the performance of chickens with bigger intensity that the cyclical exposition, having direct effect of the chronic and cyclical heat. The coefficients of metabolization of dry matter, fat and EMAn had increased with the age. The coefficients of metabolization of nutrients had been decreased by the chronic heat stress, having direct effect of the temperature only for fat. The feed restriction generated by the chronic heat caused increase of amylase activity and reduction in trypsin activity. The lipase activity increased due to direct effect of chronic heat exposure. The lesser metabolization of nutrients was associated with the worsening of the performance of the chronic heat exposed broilers, however it was not related to the worsening of performance of chickens exposed to cyclical heat stress. The reduction of the trypsin activity can be associated to the lesser digestibility of the protein caused for chronic heat stress.

Made available in DSpace on 2014-06-11T19:28:23Z (GMT). No. of bitstreams: 0 Previous issue date: 2010-07-23Bitstream added on 2014-06-13T19:16:15Z : No. of bitstreams: 1 deantonio_j_me_jabo.pdf: 657213 bytes, checksum: 374031f1e29e0df005c8fe1ed5dc8c25 (MD5) / Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) / O estresse por calor é considerado um dos fatores de maior impacto na criação de frangos de corte, já que exerce grande influência no desempenho e na qualidade da carcaça, bem como no perfil de expressão de genes relacionados ao crescimento e desenvolvimento animal. Assim, o presente trabalho objetivou verificar o efeito da temperatura e da restrição alimentar no desempenho, composição de carcaça e nos padrões de expressão de genes envolvidos na regulação do crescimento e desenvolvimento (GHR, IGF-I e IGF-IR), aos 21 e 42 dias de idade dos frangos de corte pela técnica de PCR quantitativo em tempo real. Os resultados evidenciaram que a restrição alimentar imposta pela exposição ao calor reduziu o desempenho e rendimento de peito e aumentou o rendimento de coxas+sobrecoxas. O estresse por calor e o consumo de ração alteraram o rendimento de asas, mas não influenciaram o rendimento de carcaça e de gordura abdominal. O calor aumentou o teor de extrato etéreo no peito e nas asas e diminuiu o de matéria mineral nas coxas+sobrecoxas. Independente da temperatura de criação, a restrição alimentar exerce um efeito negativo na expressão do gene do GHR no fígado das aves jovens, sem maiores reflexos na expressão do gene do IGF-I. O estresse térmico afeta a expressão dos genes hepáticos GHR e IGF-I somente nas aves adultas, as quais apresentam perfil inverso ao das aves restritas pelo “pair-feeding”. A reação de amplificação do gene IGF-IR apresentou formação de produtos de amplificação inespecíficos, assim como formação de estruturas secundárias das moléculas dos iniciadores (dímeros e “hairpins”), o que impossibilitou a análise dos resultados / Heat stress is considered one of greatest impact factors on broiler chicken production, once that it has great influence on the performance and carcass quality, as well as on the expression profile of genes related to growth and animal development. Thus, the present work had the objective of verifying the effect of temperature and food restriction on the performance, carcass composition and expression pattern of genes involved in the growth regulation and development (GHR, IGF-I and IGF-IR), at 21 and 42 days of age of the broilers using the PCR quantitative real-time technique. The results showed that the food restriction imposed by heat exposure, affected negatively the performance and breast yield and, positively, the tight and drumstick yield. The heat stress and feed intake altered the yield of wings, but did not affect carcass yield and abdominal fat. The heat increased both ether extract content in the breast and wings and reduced the mineral matter content in the tights and drumsticks. Independent of temperature, in the young bird’s livers, food restriction endorses a negative effect on the GHR gene expression, without greatest reflects on IGF-I expression. Only on adult bird’s, thermal stress affects the expression of GHR and IGF-I hepatic genes, that exhibit an inverse profile of restricted pair-feeding birds. The amplification reaction of IGF-IR gene showed both formation of nonspecific amplification products, as well as secondary structures of the primers of the molecules (dimerous and hairpins), precluding the analysis of results

Resumo: Foram realizados dois experimentos com objetivo de avaliar os efeitos da temperatura de criação, dos consumos de ração e de energia sobre o desempenho, composição bromatológica da carcaça e concentrações de alguns componentes do metabolismo intermediário no plasma e fígado de frangos com potencial genético de crescimento diferenciado. No primeiro experimento, 600 pintos de corte, machos, foram alojados em esquema fatorial 2 x 3, correspondendo a dois grupos genéticos: um da Embrapa Suínos e aves (PCLC), sob acasalamento ao acaso, desde 1984, e outro comercial (ROSS 308). O outro fator foi representado por três grupos de manejo, diferenciados pela prática de fornecimento de ração e temperatura de criação, sendo dois com ração à vontade e submetidos a duas temperaturas distintas (uma termoneutra, 23RA, e outra de estresse por calor, 32RA), e um terceiro grupo utilizado como controle (23RR) com temperatura termoneutra e consumo de ração equivalente, em pair-feed diário com o grupo 32RA. No segundo experimento, 600 pintos de corte, machos, foram alojados em esquema fatorial 2 x 3, correspondendo a dois grupos genéticos, os mesmos da descrição para o primeiro experimento, e três níveis de energia metabolizável da ração (2.950, 3.200 e 3.450 kcal/EM/kg). Frangos de corte mantidos sob altas temperaturas e consumo de ração à vontade apresentam carcaças com menor quantidade de proteína e maior deposição de gordura. Linhagens de maior potencial genético para crescimento são mais adequadas para produzir carcaças com maior rendimento e quantidade de proteína, menor deposição de gordura e maior rendimento de peito. As diferenças observadas nos níveis dos metabólitos permitem concluir que tanto a temperatura elevada quanto a redução do consumo de ração provocam alterações significativas no direcionamento de nutrientes para o metabolismo relacionado com a deposição de gordura. / Abstract: The objective of this study was to evaluate the effect of temperature, feed consumption, and metabolizable energy consumption on performance, carcass yield, carcass composition and levels of intermediate metabolites in plasma and liver of broiler chickens. Two experiments were performed. In the first trial, 600 male-chicks from two broiler strains (ROSS 308 and PCLC, a control line kept by Embrapa since 1984) were housed considering a factorial design 2 x 3 (strains x management practices). The management consisted of two groups submitted to ad libitum feed consumption, with a temperature difference (neutral, 23RA or heat stres, 32RR), and a third group (23RR) under neutral temperature and controlled feed consumption (daily pair-feed to 32RR). In the second trial, 600 male broiler-chicks were housed considering a factorial design 2 x 3 (strains, as in trial 1 x diet energy levels (2,950; 3,200 and 3,450 kcal/EM/kg). At 42 days of age, the birds were processed in order to access body composition. The results showed that birds raised at high temperatures and ad libitum feed consumption produce a carcass with lower protein content and higher abdominal fat deposition. The use of high growth potential strains showed to be the choice in order to obtain carcasses with higher protein concentration, lower fat deposits, and higher breast yield. It is possible to conclude that both high temperature and reduced feed consumption do produce significant effects on the direction of nutrients for fat deposition. / Orientador: Renato Luis Furlan / Coorientador: Poliana Fernanda Giachetto / Banca: Vera Maria Barbosa de Moraes / Banca: Danísio Prado Munari / Banca: Fabiano Dahlke / Banca: Ricardo de Albuquerque / Doutor

Thesis (M. Sc. Agriculture (Animal Production)) -- University of Limpopo, 2019 / Two experiments were conducted to determine the effect of dietary methionine level on productivity and carcass characteristics of Ross 308 broiler chickens. In each experiment, the diets were isocaloric and isonitrogenous but with different dietary methionine levels. Five diets were formulated to contain dietary methionine levels of 4, 5, 6, 8 or 9g/kg DM. The first experiment commenced with 300 unsexed Ross 308 broiler chickens with initial average live weights of 42 ± 2g per chicken. The chickens were randomly assigned to five treatments with five replications, resulting in 25-floor pens with 12 chickens per replicate. The second experiment commenced with 150 male Ross 308 broiler chickens with initial average live weight of 637 ± 12g per chicken. The chickens were randomly assigned to five treatments with three replications, resulting in 15-floor pens with 10 chickens per replicate. A complete randomized design was used in each experiment. Data was analysed using the General Linear Model (GLM) procedures of the statistical analysis of variance, Version 9.3.1 software program. Where there were significant differences, mean separation was done using the Tukey test at the 5% level of significance. A quadratic regression model was used to determine the optimal productivity of the chickens while a linear model was used to determine the relationships between dietary methionine level and responses by the chickens in the variables measured. The treatments for the first experiment were UM4 (4g methionine/kg DM), UM5 (5g methionine/kg DM), UM6 (6g methionine/kg DM), UM8 (8g methionine/kg DM) and UM9 (9g methionine/kg DM). Feed intake, growth rate, feed conversion ratio (FCR), metabolisable energy intake and nitrogen retention of unsexed Ross 308 broiler chickens aged one to 21 days were not affected (P>0.05) by dietary methionine level. Similarly, dietary methionine level did not have any effect (P>0.05) on diet crude protein (CP), acid detergent fibre (ADF), neutral detergent fibre (NDF) and fat digestibilities in unsexed Ross 308 broiler chickens aged 14 to 21 days. Dietary methionine level did not have any effect on live weights of broiler chickens at 21 days. Live weights of unsexed Ross 308 broiler chickens aged 7 or 14 days were not improved (P>0.05) by increasing dietary methionine level from 4 to 9g/kg DM. Crop, gizzard and small intestine weights and crop, proventriculus and gizzard digesta pH values of unsexed Ross 308 broiler chickens aged 21 days were not affected v (P>0.05) by dietary methionine level. Similarly, dietary methionine level did not improve (P>0.05) caecum and large intestine lengths of unsexed Ross 308 broiler chickens aged 21 days. However, dietary methionine level affected (P<0.05) dry matter (DM) and ash digestibilities of unsexed Ross 308 broiler chickens aged 14 to 21 days. Proventriculus and large intestine weights, gastrointestinal tract and small intestine lengths of unsexed Ross 308 broiler chickens aged 21 days were improved (P<0.05) by increasing dietary methionine level. In addition, increasing dietary methionine level increased (P<0.05) small and large intestine digesta pH values of broiler chickens aged 21 days. Thus, dry matter digestibility, live weights at day 7 ad 14, caecum length, large intestine length and digesta pH were optimized at different dietary methionine levels of 7.26, 5.29, 4.99, 6.80, 4.84 and 6.37g/kg DM feed, respectively. The treatments for the second experiment were MM4 (4g methionine/kg DM), MM5 (5g methionine/kg DM), MM6 (6g methionine/kg DM), MM8 (8g methionine/kg DM) and MM9 (9g methionine/kg DM). Dietary methionine level did not have effect (P>0.05) on feed intake of male Ross 308 broiler chickens aged 6 weeks. However, dietary methionine level improved (P<0.05) feed intake of male Ross 308 broiler chickens aged 4 or 5 weeks. Live weights of male Ross 308 broiler chickens aged 28 days were not affected (P>0.05) by dietary methionine level. However, live weights of male Ross 308 broiler chickens aged 35 and 42 days were affected (P<0.05) by dietary methionine level. Similarly, dietary methionine level affected (P<0.05) DM, CP, ADF, NDF, fat and ash digestibilities of male Ross 308 broiler chickens aged 35 to 42 days. Thus, dietary methionine levels of 6.93, 7.70, 6.85 and 11.27g/kg DM optimized dry matter, CP and fat digestibilities, and live weight of male broiler chickens aged 42 days. Dietary methionine level did not affect (P>0.05) FCR, growth rate and metabolisable energy intakes of male Ross 308 broiler chickens. Increasing dietary methionine level from 4 to 9g/kg DM improved (P<0.05) nitrogen retention of male Ross 308 broiler chickens aged 22 to 42 days. Dietary methionine level did not have any effect (P>0.05) on proventriculus, gizzard, caecum and large intestine weights, caecum, small and large intestine lengths, and crop, gizzard, caecum and large intestine digesta pH values of male Ross 308 broiler chickens aged 42 days. Crop and small vi intestine weights and gastrointestinal tract lengths of male Ross 308 broiler chickens aged 42 days were improved (P<0.05) by dietary methionine level. Similarly, dietary methionine level affected (P<0.05) proventriculus and small intestine digesta pH values of male Ross 308 broiler chickens aged 42 days. Thus, dietary methionine levels of 6.558 and 7.851g/kg DM optimized broiler chicken crop weight and GIT length. Dietary methionine level affected (P<0.05) carcass organ weights of male Ross 308 broiler chickens aged 42 days. Increasing dietary methionine level increased chicken breast meat weight. However, there was no clear trend for the other carcass organs. Meat flavour and shear force values of male Ross 308 broiler chickens aged 42 days were not affected (P>0.05) by dietary methionine level. However, dietary methionine level affected meat tenderness and juiciness. Thus, dietary methionine levels of 10.09 and 13.32g/kg DM optimized broiler chicken meat tenderness and juiciness. . / National Research Foundation (NRF) and VLIROUS

L’entérite nécrotique aviaire causée par Clostridium perfringens est une maladie économiquement dévastatrice et celle-ci est en émergence dans les troupeaux de poulets de chair éliminant l’usage des antibiotiques. À ce jour, aucune alternative en élevage ne permet de prévenir efficacement la maladie et un contrôle par une stratégie vaccinale serait des plus prisé. Une approche par génomique comparative jumelée à la vaccinologie réverse soustractive et comparative identifiant des protéines bactériennes de surface immunogènes figure parmi les approches méthodologiques des plus prometteuses pour le développement rapide d’un vaccin efficace. Une étude génomique comparative réalisée sur 48 souches de C. perfringens provenant de poulets de chair en santé ou affectés par l’entérite nécrotique a permis d’établir que les génomes analysés étaient composés de 155 700 protéines distinctes, où 13% étaient extracellulaires, 65% cytoplasmiques et 22% membranaires. L’évaluation du pouvoir immunogène de ces protéines à l’aide de l’outil de prédiction VaxiJen v.2.0 a permis d’identifier 4 catégories de scores pour les protéines identifiées, allant de 0,5 (seuil minimal recommandé) à 1,5. Les protéines présentant les scores les plus élevés ont été majoritairement associées à des localisations extracellulaires. La combinaison du score d’immunogénicité et de la localisation cellulaire des protéines analysées a mené à la sélection de 12 protéines candidates vaccinales, la plupart d’entre elles étant de fonction hypothétique. Une description plus approfondie de ces protéines permettra de mieux définir leur fonction, d’évaluer leur potentiel antigénique réel en caractérisant leur interaction avec le système immunitaire de la volaille et ultimement, d’évaluer leur rôle probable dans la pathogénie de l’entérite nécrotique. / Avian necrotic enteritis caused by Clostridium perfringens is a disease with a major economical impact, generating losses up to 6 billion dollars for the poultry industry worldwide. This disease appears in broiler chicken flocks that no longer employ the use of antibiotics. To date, no alternative method allows for the efficient prevention of necrotic enteritis (NE) and a control by a vaccinal strategy would be mostly prized. A comparative genomics approach as well as comparative and subtractive reverse vaccinology identifying immunogenic bacterial surface proteins is one of the most promising methodologies for the rapid development of an efficient vaccine. A comparative genomic study was performed on 48 C. perfringens strains isolated from healthy broiler chickens and from broilers affected by necrotic enteritis. From this study, it was established that the genomes analyzed were composed of 155 700 distinct proteins where 13% were predicted to have an extracellular expression, 65% at the cytoplasma level and 22% within the plasma membrane. The evaluation of the immunogenic potential of these proteins was established with the prediction software VaxiJen v2.0 for which a 0.5 threshold score allowed for the identification of four score categories among the identified proteins, from 0.5 to 1.5. For the most part, proteins with the highest scores were associated with an extracellular localisation. The combination of the immunogenicity score and localisation of the analysed proteins led to the selection of 12 vaccinal candidate proteins that were mostly identified as hypothetical. A more in-depth description of these proteins would allow the assessment of their function, the evaluation of their true immunogenic potential by characterizing their interaction with the avian immune system and ultimately, evaluate their probable role in the pathogenesis of necrotic enteritis.

Department of Animal Science / BSCAGR

Thesis (M. Sc. Agriculture (Animal Production)) -- University of Limpopo, 2021 / Two experiments were conducted to determine the effect of replacing soya bean meal with yellow mealworm larvae meal (Tenebrio molitor) in a diet on productivity, gut morphology, carcass characteristics and bone morphometrics of Ross 308 broiler chickens aged one to 42 days. In each experiment, a total of 360 Ross 308 broiler chickens were randomly assigned to the five dietary treatments, each treatment having four replications, and 18 chickens per replicate. Five diets were formulated to contain yellow mealworm replacement levels at 0, 25, 50, 75 and 100% to meet the nutrient requirements of Ross 308 broiler chickens. Data was analysed using the General Linear Model procedures of the Statistical Analysis System, Version 9.3.1 software program. Fisher’s least significant difference (LSD) test was applied for mean separation where there were significant differences (P<0.05). A quadratic regression model was used to determine the levels for optimal responses in the variables measured. The first experiment determined the effect of replacing soya bean meal with yellow mealworm larvae meal on productivity and gut morphology of unsexed Ross 308 broiler chickens aged one to 21 days. Replacement of soya bean meal with yellow mealworm meal in a diet had no effect (p > 0.05) on feed intake, growth rate, FCR, live body weight, ME intake and nitrogen retention of unsexed Ross 308 broiler chickens aged one to 21 days. Replacing soya bean meal with yellow mealworm meal in a diet did not affect (p > 0.05) caecum weight of unsexed Ross 308 broiler chickens. However, replacing soya bean meal with yellow mealworm meal in a diet increased (p < 0.05) gastro intestinal tract, crop, ileum and large intestine weights. Crop and ileum lengths of unsexed Ross 308 broiler chickens aged 21 days were not affected (p > 0.05) by replacement of soya bean meal with yellow mealworm meal in the diet. However, replacing soya bean meal with yellow mealworm meal in a diet increased (p < 0.05) gizzard, caecum and large intestine lengths of unsexed Ross 308 broiler chickens aged 21 days. Yellow mealworm meal in a diet did not affect (p > 0.05) gut organ digesta pH values of unsexed Ross 308 broiler chickens aged 21 days. The second experiment determined the effect of replacing soya bean meal with yellow mealworm meal in a diet on productivity, gut morphology, carcass characteristics and bone morphometrics of Ross 308 broiler chickens aged 22 to 42 days. Replacement of soya bean meal with yellow mealworm meal in a diet did not affect (p > 0.05) growth rate, FCR, ME intake and nitrogen retention of male Ross 308 broiler chickens aged 22 to 42 days. However, replacing soya bean meal with yellow mealworm meal in a diet affected (p < 0.05) feed intake and live body weight of male Ross 308 broiler chickens aged 22 to 42 days. Broiler chickens on diets containing 75 or 100% yellow mealworm meal had higher (p < 0.05) intakes than those on diets containing no yellow mealworm meal. Similarly, male broiler chickens on diets having 50% yellow mealworm meal had higher (p < 0.05) live body weights than those on diets containing no yellow mealworm. Quadratic equations indicated that feed intake and live body weight of male Ross 308 broiler chickens were optimized at yellow mealworm meal replacement levels of 13 and 61%, respectively. The present study showed that replacing soya bean meal with yellow mealworm meal in a diet did not affect (p > 0.05) gut organ digesta pH values, gut organ weights, gut organ lengths, meat colour, meat pH values, bone morphometric values, carcass part weights and meat sensory attributes of male Ross 308 broiler chickens aged 42 days. However, meat from chickens on diets containing yellow mealworm meal was softer (p < 0.05) than meat from chickens on diets having 100% soya bean meal. It is concluded that soya bean meal can be replaced with yellow mealworm larvae meal in a diet at 25, 50, 75 and 100% levels without having adverse effects on production and carcass characteristics of Ross 308 broiler chickens aged one to 42 days

Thesis (M.Sc. (Animal Production)) -- University of Limpopo, 2023 / Two experiments were conducted to determine the effect of citric acid inclusion level in the diet on growth performance, carcass weight, tibia bone breaking strength and ash content of male Ross 308 broiler chickens aged one to 35 days. The first experiment determined the effect of citric acid inclusion level in the diet on growth performance traits of male Ross 308 broiler chickens aged one to 21 days. The experiment commenced with 200 male day-old Ross 308 broiler chickens with an initial average live weight of 40±1.6g per chick. The chicks were assigned to five treatment groups in a completely randomized design, each replicated five times, and each replicate having ten chicks. The citric acid inclusion levels were at 0, 12.5, 25 or 50g per kg DM of feed. The second experiment determined the effect of citric acid inclusion level in the diet on growth performance, carcass weight, tibia bone breaking strength and ash content traits of male Ross 308 broiler chickens aged 22 to 35 days. The experiment commenced with 180 male Ross 308 broiler chickens aged 22 days. The chickens were assigned to four treatment groups, each having three replicate pens of eight chickens per replicate in a completely randomized design. Data was analysed using the General Linear model (GLM) procedures of the Statistical Analysis of System, version 9.3.1 software program. Where there were significant differences (P<0.05) between the treatment means, Tukey Multiple Comparison Test was used for mean separation. Citric acid inclusion in the starter diets improved (P<0.05) live weight and growth rate of male Ross 308 broiler chickens aged one to 21 days. Citric acid inclusion in the starter diets did not affect (P>0.05) daily feed intake, body weight gain and feed conversion ratio of male Ross 308 broiler chickens aged one to 21 days. The inclusion of citric acid did affect (P<0.05) live weight, body weight gain, feed conversion ratio and growth rate of chickens aged 22 to 35 days. Citric acid inclusion levels used in the present study influenced (P<0.05) DM and CP digestibility, ME intake and N-retention of male broiler chickens aged 22 to 35 days. The results of the current study showed that citric acid inclusion in a diet improved (P<0.05) chicken bone morphology. Thus, positive relationships were observed between citric acid inclusion level and right tibia bone weight, diameter, calcium, phosphorous and Magnesium contents of chicken bones aged 35 days. There were positive relationships between citric acid inclusion level and breast weight of male Ross 308 broiler chickens aged 35 days. Further studies are recommended to ascertain these findings. / National Research Foundation (NRF)

<p>Cilj istraţivanja bio je da se ispita uticaj svetlosnog programa (konstantnog &ndash; KS i opadajuĆe- rastuĆeg - ORS), gustine obroka i gustine naseljenosti i njihove interakcije na proizvodne i klaniČne osobine brojlerskih piliĆa, kvalitet mesa i o&scaron;teĆenje tabanskih jastuČiĆa.<br />Istraţivanja su sprovedena na farmi piliĆa Donji Crnci nadomak Podgorice u tri ogleda: u prvom su ispitivani efekti svetlosnog programa i gustine obroka, u drugom programi svetla i gustina naseljenosti piliĆa, a u treĆem program svetla i gustina obroka (sme&scaron;e sa standardnim i poveĆanim sadrţajem energije i proteina, pri Čemu je odnos energija : protein bio konstantan). U svakom ogledu bilo je 320 piliĆa linijskog hibrida Cobb 500, me&scaron;anih po polu, a ogledi su trajali 42 dana. U sva tri ogleda formirane su po Četiri grupe sa Četiri ponavljanja i u svakoj grupi bilo je 80 jednodnevnih piliĆa.<br />Proizvodni parametri (telesna masa, utro&scaron;ak hrane i uginuĆa) praĆeni su u svim ogledima, a izraČunati su konverzija hrane i proizvodni indeks. Telesne mase su merene u uzrastu od 1; 7; 14; 21; 28; 35 i 42 dana. Utro&scaron;ak hrane utvrčivan je na kraju perioda primene pojedinih sme&scaron;a, i to: 14. dana koliČina konzumirane starter sme&scaron;e, 35. dana grovera i 42. dana fini&scaron;er sme&scaron;e. Mortalitet je odrečivan beleţenjem broja uginulih piliĆa u toku trajanja ogleda. Na osnovu telesnih masa, konverzije hrane i mortaliteta izraČunati su proizvodni indeksi.<br />Na kraju svakog ogleda, sa 6 nedelja starosti, piliĆi su izmereni pojedinaČno, izraČunati su proseci tretmana, a zatim je od svakog tretmana izdvojeno 20 piliĆa (10 mu&scaron;kih i 10 ţenskih) radi ispitivanja klaniČnih karakteristika. Pre klanja piliĆi su gladovali 12 sati. Nakon klanja i Či&scaron;Ćenja trupovi su ohlačeni na 40C u toku 24 sata, a potom obračeni kao: &bdquo;klasiČna obrada&rdquo; &bdquo;spremno za peČenje&rdquo; i &bdquo;spremno za ro&scaron;tilj&rdquo;.<br />Pri konfekcioniranju trupova izdvojena je abdominalna mast, izmerene mase obračenih trupova i dobijeni randmani. Radi utvrčivanja prinosa i udela osnovnih i sporednih delova trupa izvr&scaron;eno je rasecanje ohlačenih trupova. Ocena konformacije trupova izvr&scaron;ena je na osnovu utvrčenih apsolutnih mera: duţine piska, duţina kobilice, dubina grudi i obim bataka. Kori&scaron;Ćen je indeks koji predstavlja odnos ţive mase pre klanja i posmatrane mere (g/mm).<br />U ogledu II izvr&scaron;eno je i fiziČko ispitivanje kostiju, na femuru nakon izdvajanja od skeleta brojlera, a u ogledu III odrečeni su sadrţaj vlage, sadrţaj ukupne masti, ukupnog pepela i ukupnih proteina belog i crvenog mesa, na uzorcima mi&scaron;iĆnog tkiva grudi i karabataka od 5 mu&scaron;kih i 5 ţenskih trupova u svakom tretmanu (ukupno 20).</p><p>&nbsp;</p><p>&nbsp;</p><p>Rezultati ukazuju da svetlosni program nije statistiČki znaČajno uticao na zavr&scaron;ne telesne mase u ogledima I i II, dok su u ogledu III veĆe mase utvrčene kod piliĆa tovljenih pri KS. Primena ORS uticala je na usporavanje stope rasta u prvom periodu tova, a kao rezultat kompenzacionog porasta bez uticaja na zavr&scaron;ne mase, sem u treĆem ogledu. Bolju konverziju hrane u periodu ishrane fini&scaron;erom postigli su piliĆi u ogledu I pri ORS nego pri KS, kao i u ishrani starterom u ogledu III. Svetlosni programi u ogledu II nisu uticali na konverziju hrane i mortalitet piliĆa.<br />Gustina obroka nije uticala na zavr&scaron;ne mase, konverziju hrane, mortalitet i proizvodni indeks. Kod piliĆa hranjenih obrocima sa vi&scaron;im sadrţajem proteina i energije utvrčene su veĆe telesne mase na kraju tova (P&lt;0.01). Gustina obroka u ogledima I i III nije imala uticaj na konverziju hrane, mortalitet i proizvodni indeks. Znatno je veĆa zavr&scaron;na masa piliĆa tovljenih pri gustini naseljenosti od 12 grla/m2 (2667,08 g) nego pri 17 grla/m2 (2435,76 g) i razlike su bile statistiČki visoko znaČajne. Nije utvrčen uticaj gustine naseljenosti na konverziju hrane, mortalitet i proizvodni indeks.<br />Ispitivani faktori (svetlo, gustina obroka i gustina naseljenosti) nisu uticali na randmane, ali jesu interakcije nekih od njih. U svim ogledima utvrčen je manji udeo abdominalne masti pri ORS nego pri KS, ali bez statistiČke znaČajnosti. Gustina obroka i gustina naseljenosti nisu uticali na sadrţaj abdominalne masti. VeĆi je sadrţaj abdominalne masti u svim ogledima bio kod ţenskih nego mu&scaron;kih piliĆa. Udeo grudi veĆi je pri KS nego pri ORS, ali su razlike statistiČki znaČajne samo u treĆem ogledu. Gustina obroka i gustina naseljenosti nisu uticale na udeo grudi. Udeo bataka bio je veĆi kod piliĆa tovljenih pri ORS nego pri KS, ali su razlike statistiČki znaČajne samo u prvom ogledu. Udeo karabataka u prvom i drugom ogledu nije se znaČajno razlikovao izmeču svetlosnih programa, dok je u treĆem bio veĆi pri ORS nego pri KS (P&lt;0.01). Nije utvrčen uticaj gustine naseljenosti i gustine obroka na udeo bataka i karabataka. Kod mu&scaron;kih piliĆa utvrčen je veĆi udeo bataka, a kod ţenskih karabataka.<br />Relativne vrednosti mera konformacije nisu bile pod uticajem svetlosnih programa i gustine obroka, osim &scaron;to je pri manjoj gustini naseljenosti relativna vrednost dubine grudi bila veĆa (P&lt;0.05). Na o&scaron;teĆenje tabanskih jastuČiĆa nije utvrčen uticaj svetla i gustine obroka, ali su piliĆi pri veĆim gustinama naseljenosti, poČev od 28. dana, imali znatno veĆa o&scaron;teĆenja tabanskih jastuČiĆa nego pri manjoj gustini.<br />Svetlosni program i gustina naseljenosti nisu uticali na fiziČke osobine femura: povr&scaron;inu preseka, silu loma i specifiČnu silu loma. Mu&scaron;ki piliĆi imali su veĆi povr&scaron;inu preseka i silu loma femura, razlike za povr&scaron;inu preseka su statistiČki znaČajne, ali ne i za silu loma.<br />Procenat masti u tamnom mesu bio je veĆi, a proteina niţi pri ORS nego pri KS. Ostali parametri hemijske analize tamnog i belog mesa nisu bili pod uticajem svetlosnih programa. Gustina obroka nije imala uticaj na hemijski sastav mesa grudi i karabataka.<br />Na osnovu svega iznesenog moţe se zakljuČiti da su sva tri faktora (svetlosni program, gustina obroka i gustina naseljenosti), kao i njihove interakcije, uticali na brojne proizvodne parametre i kvalitet mesa brojlera, pa i na njihovu dobrobit u razliČitim fazama tova. ImajuĆi u vidu ogromne razlike u efektima koji se mogu postiĆi pri razliČitim kombinacijama ovih Činilaca, rezultati ovih i sliČnih istraţivanja treba da olak&scaron;aju izbor tehnologije koja Će davati najbolje ekonomske efekte u tovu piliĆa. Takoče, tamo gde su rezultati nejasni ili kontradiktorni, treba nastaviti istraţivanja na veĆem broju jedinki i fokusirati se na ekonomski vaţnije proizvodne i klaniČne osobine piliĆa.</p> / <p>The purpose of the research was to investigate the effect of the lighting program (constant &ndash; KS and intermittent &ndash; ORS), feed density and stocking density and their interaction with production and slaughterhouse characteristics of broiler chicken, meat quality and feet pad damage.<br />The research was conducted on a chicken farm Donji Crnci in the vicinity of Podgorica, in three trials: the first trial focused on effects of the lighting program and feed density, the second on the lighting program and stocking density and the third on the lighting program and the feed density (mixes with standard and increased content of energy and protein, with a constant energy:protein ratio). Each of the trials included 320 chicken of the Cobb 500 hybrid line, mixed sexes; trials lasted for 42 days. In all three trials, four groups were formed with four repetitions and each group included 80 one-day chickens.<br />Production parameters (weight, feed consumption and deaths) were monitored in all trials and feed conversion ratio and production index were calculated. Weight was measured when broilers were 1; 7; 14; 21; 28; 35 and 42 days old. Feed consumption was determined at the end of the use of specific mixes, as follows: 14th day the quantity of starter mix consumed, 35th day the quantity of grower mix consumed and 42nd day quantity of the finisher mix. Mortality rate was determined by recording the number of chicken that died during the trial. Production indices were calculated based on weight, food conversion ratio and mortality.<br />At the end of all trials, at 6 weeks of age, the chickens were measured individually, group averages were calculated and then by random sampling, 20 chicken were taken from each group (10 males and 10 females) in order to analyse slaughter characteristics. Pre-slaughter fasting lasted for 12 hours. After slaughter and cleaning, the carcasses were cooled to 40C over 24 hours and then processed as: &ldquo;classical processing&rdquo;, &ldquo;ready to grill&rdquo; and &ldquo;barbecue ready&rdquo;.<br />In carcass processing, abdominal fat was taken out, weight of processed carcases measured and carcass yield values were obtained. In order to determine the yield and share of the main and secondary parts of the carcass, cooled carcasses were cut out. Evaluation of carcass conformation was done on the basis of absolute measures of the following: metatarsus length, keel length, breast depth and drumsticks circumference. Index representing the ratio of live weight before slaughter and measure observed was used (g/mm).<br />In Trial II, physical examination of bones was done, on femur, upon separation from the broiler skeleton, and in Trial III moisture content, total fat content, total ash content and total protein of white and red meat was determined on samples of muscle tissue of breasts and thighs of 5 male and 5 female carcasses in each of the treatments (20 in total).<br />The results show that lighting program had no statistically significant effect on final body weight in Trials I and II, while in Trial III, higher weights were recorded in chicken fattened under KS. ORS resulted in slowing down of the growth rate in the first fattening period, and as a result of compensatory growth it had no effect on final weights, except in the third trial. Better food conversion ratio in the period of finisher mix diet was achieved by chicken in Trial I under ORS than those under KS, as well as in starter mix diet in Trial III. Lighting program in Trial II had no effect on food conversion ratio and mortality of chicken. Feed density did not have an effect on final weights, feed conversion, mortality and production index. In chicken fed with meals with higher protein and energy content, higher weight was recorded at the end of the fattening period (P&lt;0.01). Feed density in Trials I and III did not have an effect on feed conversion ratio, mortality and production index. Significantly higher weight was recorded in chicken fattened at the stocking density of 12 animals/m2 (2667.08 g) than at the density of 17 animals/m2 (2435.76<br />8<br />g) and differences had statistically high significance. No effect of stocking density was identified in terms of feed conversion, mortality and production index.<br />Factors analysed (light, feed density and stocking population) did not have an effect on carcass yield, but interactions of some of them did. In all the trials, a smaller share of abdominal fat was established under ORS than under KS, but it was not statistically significant. Feed density and stocking density had no effect on abdominal fat content. Higher abdominal fat content in all trials was found in female than in male chicken. The share of breasts is larger under KS than under ORS, but differences are statistically significant only in the Trial III. Feed density and stocking density had no effect on the share of breasts in the carcass. The share of drumsticks was higher in chicken fattened under ORS than those under KS, but differences were statistically significant only in the Trial I. The share of thighs in Trials I and II did not differ significantly between lighting programs, while in the Trial III it was higher under ORS than under KS (P&lt;0.01). Effect of stocking density and feed density on the share of drumsticks and thighs was not determined. Higher share of drumsticks was determined in male chicken while in female, the share of thighs was higher.<br />Relative values of conformation measures were not influenced by the light programs and feed density, except that in lower stocking density the relative value of the breast depth was higher (P&lt;0.05). Effect of light and feed density on feet pads damage was not determined, but chicken in higher stocking density, as of 28th day, had significantly higher feet pad damage than those in lower stocking density.<br />Light program and stocking density did not have an effect on physical properties of femur: cross-sectional area, breaking force and specific breaking force. Male chicken had larger cross-sectional area and femur breaking force, differences in cross-sectional area were statistically significant, but this was not the case with the breaking force.<br />Percentage of fat in dark meat was higher and that of proteins lower under ORS than under KS. Other parameters of the chemical analysis of dark and white meat were not under the influence of the light programs. Feed density had no effect on chemical composition of breasts and thighs meat.<br />Based on all stated above, it can be concluded that all three factors (light program, feed density and stocking density) as well as their interactions had effect on numerous production parameters and quality of broiler meat, including on their welfare in different fattening phases. Taking into account enormous differences in effects that may be achieved in different combinations of these factors, the results of these and similar researches should make easier the selection of the technology that would yield the best economic effects in chicken fattening. Furthermore, where results are unclear or contradictory, further research should be conducted on a larger number of animals and focus on economically more significant production and slaughterhouse characteristics of chicken.</p>

Le Staphylococcus aureus résistant à la méthicilline (SARM) est un enjeu majeur en santé publique. Il est responsable d’une grande variété d’infections. Les “Livestock Associated-MRSA” (LA-MRSA) sont des SARM ayant comme origine les animaux de production tels le porc ou la volaille. Ils constituent un risque de transmission à l’humain via la chaîne alimentaire. Les LA-MRSA peuvent former du biofilm ce qui augmente leur tolérance aux stress environnementaux. Le biofilm est partiellement régulé par le système Agr. Il n’existe aucune donnée sur les ‘LA-MRSA’ d’origine aviaire au Québec. Les objectifs de ce projet étaient : (i) de déterminer la prévalence de ces SARM dans la viande de poulet et le poulet à griller de la province de Québec et (ii) de caractériser les isolats retrouvés. La collecte d’échantillons s’est effectuée dans 43 épiceries (309 cuisses et pilons de poulet) et dans deux abattoirs (échantillons nasaux et fécaux de 200 poulets) de la Montérégie. La prévalence de SARM a été évaluée à 1.29% (IC 95%: 0.35-3.28) et 0% dans la viande et les oiseaux respectivement. Les isolats testés se sont révélés résistants aux bêta-lactamines (n=15), à la tétracycline (n=10), à l’oxytétracycline (n=10), à la spectinomycine (n=10) et à la tobramycine (n=1). Le typage a révélé deux clones différents (ST398-V, n=10; et ST8-IVa ’USA300’, n=5). La présence de gènes de résistance aux antibiotiques (blaZ, blaR, blaI, erm(A), lnu(A), aad(D), fosB, tet(K), tet(L) et spc) ainsi que plusieurs gènes codant pour l’évasion du système immunitaire (IEC), la production de toxines ou encore pour la production de biofilm ont aussi été détectés. Une forte production de biofilm a été observée pour la majorité des isolats (n=11) à l’exception de certains isolats ST398. Le taux d’expression du système Agr n’a révélé aucune différence particulière entre les SARM testés. Pour conclure, nos données indiquent une faible prévalence de SARM chez la volaille et la viande de poulet. Les isolats ont été catégorisés en deux génotypes, dont un portant plus de gènes de résistance aux antibiotiques (ST398) et l’autre possédant plus de gènes de virulence (ST8). / Methicillin-resistant Staphylococcus aureus (MRSA) is of critical concern for public health. It is responsible for a wide range of infections. Livestock-Associated MRSA (LA-MRSA) refers to MRSA of animal origin, like pork, cattle or poultry, and constitutes a risk for transmission to humans. LA-MRSA can also form biofilms enhancing their environmental survival. Biofilms are partly regulated by the Agr system. There is no data on LA-MRSA of poultry origin from Québec, Canada. The objectives of this project were: (i) to determine the prevalence of LA-MRSA from chicken meat and broiler chickens from the Province of Quebec, and (ii) to molecular characterize these MRSA. A total of 309 chicken drumsticks and thighs were randomly collected. In addition, nasal swabs and caeca samples from 200 chickens were randomly collected at slaughterhouses. LA-MRSA was recovered from 1.29% (95% CI: 0.35-3.28) of chicken meat samples but none were recovered from poultry. Antibiotic susceptibility testing revealed resistances to beta-lactam antibiotics (n=15), tetracycline (n=10), oxytetracycline (n=10), spectinomycin (n=10) and tobramycin (n=1). Molecular typing revealed two types of clones (ST398-V, n=10; and ST8-IVa ’USA300’, n=5). DNA microarrays determined the presence of the antibiotic resistant genes blaZ, blaR, blaI, erm(A), lnu(A), aad(D), fosB, tet(K), tet(L) and spc. In addition, genes encoding for toxins, biofilm formation and the human-associated Immune-Evasion-Cluster (IEC) were also detected. High biofilm production was observed in most isolates (n=11) with the exception of some ST398. Quantitative PCR of Agr expression revealed no specific difference among MRSA isolates. To conclude, our data show that the prevalence of the MRSA lineages ST398 and ST8 is low in broilers and chicken meat in the Province of Quebec. ST398 demonstrated more antibiotic resistant genes while ST8 harboured more virulence genes.