Thesis (M.S.)--Miami University, Dept. of Chemistry and Biochemistry, 2004. / Title from first page of PDF document. Includes bibliographical references (p. 57-64).

Este estudo investiga processos de composição musical vinculados ao registro sonoro e à produção de um CD: Como o uso de registros sonoros se coaduna aos processos composicionais coletivos? Quais as implicações de compor e gravar músicas com adolescentes? Como os adolescentes desencadeiam processos composicionais quando os objetivos estão voltados para a produção de um CD? A pesquisa foi realizada com um grupo de 10 adolescentes, no âmbito de uma Oficina oferecida como atividade extracurricular numa escola da rede pública municipal em Gravataí-RS. O referencial teórico apoiou-se em dois eixos: composição musical na educação musical (SANTOS, 1994; SWANWICK, 1979; McDONALD e MIELL, 2000; FAUTLEY, 2004) e música e tecnologias (IAZZETTA, 1996, 1997, 2001; RODRIGUES, 2002 e CARVALHO, 1999). A metodologia adotada foi a pesquisaação na concepção de Pesquisa-Ação Integral (PAI) de André Morin. Entre as principais conclusões está a de que os processos composicionais constituíram-se para além de estruturações musicais, sendo constantemente permeados pelo conjunto de inter-relações sócio-afetivas do grupo. O registro das composições favoreceu o feedback auditivo para os adolescentes, podendo dessa forma, estabelecer novas referências estéticas de percepção musical sobre as próprias composições. Além disso, a produção do CD se configurou como um resultado tangível do fazer musical do grupo, no qual a identidade coletiva e a individual se fundiram. / This study investigates the processes of musical composition linked to the sound record and the production of a CD: how do the use of sound records join to collective compositional processes? What are the implications of composing and recording musics with adolescents? How do they develop compositional processes when the objectives are focused on the production of a CD? The research was carried out on a group of 10 adolescents, in a workshop offered as an extra class activity in a public school in the municipality of Gravataí, RS. The theoretical framework was supported in two axes: musical composition in the musical education (SANTOS, 1994; SWANWICK, 1979; McDONALD e MIELL, 2000; FAUTLEY, 2004) and music and technologies (IAZZETTA, 1996, 1997, 2001; RODRIGUES, 2002 e CARVALHO, 1999). The method of investigation was the action research in the view of André Morin’s Integral Action Research. Among the principal conclusions, it is that the compositional processes resulted beyond the musical structures, being constantly surrounded by a social-affective inter-relations set of the group. The recording of the compositions supported the auditory feedback to the adolescents, becoming possible to them, this way, to set up new aesthetic references of musical understanding about their own compositions. Moreover, the CD production became a tangible result of music making of the group, in which the collective and individual identity fused together.

Este estudo investiga processos de composição musical vinculados ao registro sonoro e à produção de um CD: Como o uso de registros sonoros se coaduna aos processos composicionais coletivos? Quais as implicações de compor e gravar músicas com adolescentes? Como os adolescentes desencadeiam processos composicionais quando os objetivos estão voltados para a produção de um CD? A pesquisa foi realizada com um grupo de 10 adolescentes, no âmbito de uma Oficina oferecida como atividade extracurricular numa escola da rede pública municipal em Gravataí-RS. O referencial teórico apoiou-se em dois eixos: composição musical na educação musical (SANTOS, 1994; SWANWICK, 1979; McDONALD e MIELL, 2000; FAUTLEY, 2004) e música e tecnologias (IAZZETTA, 1996, 1997, 2001; RODRIGUES, 2002 e CARVALHO, 1999). A metodologia adotada foi a pesquisaação na concepção de Pesquisa-Ação Integral (PAI) de André Morin. Entre as principais conclusões está a de que os processos composicionais constituíram-se para além de estruturações musicais, sendo constantemente permeados pelo conjunto de inter-relações sócio-afetivas do grupo. O registro das composições favoreceu o feedback auditivo para os adolescentes, podendo dessa forma, estabelecer novas referências estéticas de percepção musical sobre as próprias composições. Além disso, a produção do CD se configurou como um resultado tangível do fazer musical do grupo, no qual a identidade coletiva e a individual se fundiram. / This study investigates the processes of musical composition linked to the sound record and the production of a CD: how do the use of sound records join to collective compositional processes? What are the implications of composing and recording musics with adolescents? How do they develop compositional processes when the objectives are focused on the production of a CD? The research was carried out on a group of 10 adolescents, in a workshop offered as an extra class activity in a public school in the municipality of Gravataí, RS. The theoretical framework was supported in two axes: musical composition in the musical education (SANTOS, 1994; SWANWICK, 1979; McDONALD e MIELL, 2000; FAUTLEY, 2004) and music and technologies (IAZZETTA, 1996, 1997, 2001; RODRIGUES, 2002 e CARVALHO, 1999). The method of investigation was the action research in the view of André Morin’s Integral Action Research. Among the principal conclusions, it is that the compositional processes resulted beyond the musical structures, being constantly surrounded by a social-affective inter-relations set of the group. The recording of the compositions supported the auditory feedback to the adolescents, becoming possible to them, this way, to set up new aesthetic references of musical understanding about their own compositions. Moreover, the CD production became a tangible result of music making of the group, in which the collective and individual identity fused together.

Este estudo investiga processos de composição musical vinculados ao registro sonoro e à produção de um CD: Como o uso de registros sonoros se coaduna aos processos composicionais coletivos? Quais as implicações de compor e gravar músicas com adolescentes? Como os adolescentes desencadeiam processos composicionais quando os objetivos estão voltados para a produção de um CD? A pesquisa foi realizada com um grupo de 10 adolescentes, no âmbito de uma Oficina oferecida como atividade extracurricular numa escola da rede pública municipal em Gravataí-RS. O referencial teórico apoiou-se em dois eixos: composição musical na educação musical (SANTOS, 1994; SWANWICK, 1979; McDONALD e MIELL, 2000; FAUTLEY, 2004) e música e tecnologias (IAZZETTA, 1996, 1997, 2001; RODRIGUES, 2002 e CARVALHO, 1999). A metodologia adotada foi a pesquisaação na concepção de Pesquisa-Ação Integral (PAI) de André Morin. Entre as principais conclusões está a de que os processos composicionais constituíram-se para além de estruturações musicais, sendo constantemente permeados pelo conjunto de inter-relações sócio-afetivas do grupo. O registro das composições favoreceu o feedback auditivo para os adolescentes, podendo dessa forma, estabelecer novas referências estéticas de percepção musical sobre as próprias composições. Além disso, a produção do CD se configurou como um resultado tangível do fazer musical do grupo, no qual a identidade coletiva e a individual se fundiram. / This study investigates the processes of musical composition linked to the sound record and the production of a CD: how do the use of sound records join to collective compositional processes? What are the implications of composing and recording musics with adolescents? How do they develop compositional processes when the objectives are focused on the production of a CD? The research was carried out on a group of 10 adolescents, in a workshop offered as an extra class activity in a public school in the municipality of Gravataí, RS. The theoretical framework was supported in two axes: musical composition in the musical education (SANTOS, 1994; SWANWICK, 1979; McDONALD e MIELL, 2000; FAUTLEY, 2004) and music and technologies (IAZZETTA, 1996, 1997, 2001; RODRIGUES, 2002 e CARVALHO, 1999). The method of investigation was the action research in the view of André Morin’s Integral Action Research. Among the principal conclusions, it is that the compositional processes resulted beyond the musical structures, being constantly surrounded by a social-affective inter-relations set of the group. The recording of the compositions supported the auditory feedback to the adolescents, becoming possible to them, this way, to set up new aesthetic references of musical understanding about their own compositions. Moreover, the CD production became a tangible result of music making of the group, in which the collective and individual identity fused together.

Das Campusnetz - neue Strukturen und Funktionen Neues vom WWW-Server Umstellung der HOME-Verzeichnisse vom NFS ins AFS AFS löst NFS ab! Neue Rechnersysteme - zur allgemeinen Nutzung Superskalarrechner - Inbetriebnahme und Nutzungsmöglichkeiten PC-Integration via SAMBA-Server CD-ROM im Netz Die Programmpakete MATHEMATICA und ANSYS Ueberblick ueber /uni/global-Software Ein weihnachtliches Problem?

Notre étude s’inscrit dans le cadre des travaux en analyse de discours et consiste en une description sémiolinguistique de deux corpus, le premier issu de l’espace éditorial des médias informatisés, et le second extrait de la presse ordinaire quotidienne. Elle cherche plus précisément à démontrer, dans un premier temps, l’instabilité et la transformation au fil du temps des représentations socio-discursives à l’égard de l’écrit d’écran au sein de l’espace socio-historique français à la fin du vingtième siècle ; puis, dans un second temps, elle vise à identifier, au travers de cette fluctuation, l’orientation de stabilisation de ces mêmes représentations. Pour parvenir à cette fin, la présente étude place à son épicentre la notion d’image discursive, face visible, lisible et descriptible des représentations en question. Se limitant d’abord à un corpus de cédéroms nommés dans le métalangage naturel de “culturels” ou “grand public”, relatifs a priori à la transmission d’un savoir scientifique, elle vise à saisir l’image de l’écrit d’écran au sein de ce corpus à travers les divers degrés de didacticité que celui-ci convoque durant la courte diachronie des médias informatisés hors ligne. C’est ainsi que point, au fil de la description et des années, un paradigme de didacticité tripartite, celui-ci allant du visible à l’intelligible en passant par le lisible. Les images discursives de l’ethos des communautés scientifiques abordées par les écrits d’écran du corpus investissent à leur tour ce paradigme et sa mouvance dans le chronotope de la société française. La mobilisation de la presse ordinaire quotidienne, représentée ici par Le Monde s’effectue à des fins d’étayage du modèle initial de didacticité. À terme, ce quotidien – miroir social à part entière – interrogé à partir des mêmes notions descriptives que le corpus de cédéroms, permet non seulement de valider le modèle de départ, mais aussi, dans un souci interprétatif et causal plus étendu, d’inférer un certain nombre de représentations que la seule description du corpus de cédéroms ne nous aurait pas permis d’induire. En sus des implications didactiques [notamment dans le cadre de la formation de formateurs et de futurs enseignants] envisagées en fin de parcours, en sus aussi des prolongements descriptifs dans le cadre de l’internet, cette étude saisit un moment historique manifeste dans la mesure où elle nous permet de mieux comprendre notre rapport avec les matérialités qui nous entourent et les processus que nous mobilisons pour mieux nous les approprier. / Discourse analysis is the general research framework of this study. It consists of a semio-linguistic description of two corpora: the first is composed of computer-mediated news articles and the second of traditional daily print media. The research begins by examining the transformation and possible changes in socio-discursive written representations as presented on the computer screen in the late 20th century French socio-historical context. The next stage is to identify, through these variations, how these representations have stabilised. With this aim in mind, the study focuses on the concept of the discursive image, which is the visible, readable and describable side of such representations. We examine a corpus of CD-ROMs [so-called “cultural CD-ROMs” or “mainstream CD-ROMS”] which are designed to convey scientific knowledge, and try to analyse the image of writing-on-screen through the different levels of didactic purpose, as they appear over the short time span of offline media. This study reflects the emergence of a 3-fold model of didactic purpose: first an analysis of the visible, secondly an analysis of the legible and thirdly an analysis of the intelligible. The discursive images of the ethos of the scientific communities as they appear in writing-on-screen are also influenced by the time and space scheme specific to French society. The analysis of traditional daily print media, which is represented by Le Monde, extends the initial model of didacticity. At the end of the day, the analysis [using the same tools as for the corpus of CD-ROMs] of the mainstream press -a mirror of society- not only allows us to validate our results but also to infer some representations which the description of CD-ROMs alone has not shown. This study, apart from opening up new didactic perspectives for the training of future teachers and its implications for description on the Internet, grasps a historical moment, in the sense that it allows us to understand the relation that we have to the media and the processes that we use in order to interpret the media that surround us.

Characterization of post-translational modifications (PTMs) of therapeutic proteins is very important during the bioprocess development to maintain desired product quality and during the submission process to regulatory authorities for product approval. Monitoring glycosylation in pharmacokinetic studies can be useful to evaluate the dependence of clearance rates on different glycoforms. The cost and efficiency of characterization affect the speed to market of biopharmaceutical proteins. A reduction in the number of manual processing steps, cost of reagents and consumption of sample, as well as the time required for chemical analysis, is therefore necessary. The research presented in this thesis is focused on the potential of using microfluidic discs for automated, miniaturized, parallel and rapid sample preparation for PTM characterization of therapeutic monoclonal antibodies. Paper I describes the method development for N-linked glycosylation profiling. Several sample preparation steps have been performed in an integrated process in the microfluidic compact disc (CD). Paper II demonstrates the use of the method presented in paper I in combination with multivariate statistics for discrimination of glycosylation profiles of different therapeutic antibodies and simulation of a real case of quality control. Paper III is focused on a method for monitoring changes in glycosylation profiles of therapeutic antibodies in serum over time by incubation with an exoglycosidase enzyme. Paper IV describes the method for peptide mapping of therapeutic antibodies. In addition, recent work (unpublished results) assesses the potential of this method for methionine oxidation detection. The developed methods were fast, robust with low sample/reagent consumption. Generation of glycosylation profile data for one sample was established in approximately 2 h. The amount of samples and antigens loaded into the CD platform for one replicate was less than 0.3 μg and approximately 0.06 μg, respectively. Furthermore, considering the parallel function of the CD, conducting the analysis for 54 samples can be completed within a day. / <p>At the time of the doctoral defense, the following paper was unpublished and had a status as follows: Paper 3: Manuscript.</p>

This document defines systems of pushdown transducers. The idea of cooperating distributed grammar systems for components working on one word is adjusted for use of transducers instead of grammars. The transducers cooperate by passing output of one to input of another component. It discusses their descriptive power and equivalency between systems with arbitrary numbers of components. The main conclusion is then comparison of their descriptive power with Turing machines with regard to their translation and accepted languages.

L'essor des marqueurs moléculaires (SNPs) a révolutionné les méthodes de génétique quantitative en permettant l'identification de régions impliquées dans le déterminisme génétique des caractères (QTLs) via la génétique d'association (GWAS), ou encore la prédiction des performances d'individus sur la base de leur information génomique (GS). La stratification des populations en groupes génétiques est courante en sélection animale et végétale. Cette structure peut impacter les méthodes de GWAS et de GS via des différences de fréquence et d'effets des allèles des QTL, ainsi que par des différences de déséquilibre de liaison (LD) entre SNP et QTL selon les groupes.Pendant cette thèse, deux panels de diversité de maïs ont été utilisés, présentant des niveaux différents de structuration: le panel “Amaizing Dent” représentant les lignées dentées utilisées en Europe et le panel “Flint-Dent” incluant des lignées dentées, cornées européennes, ainsi que des lignées admixées entre ces deux groupes.En GS, l'impact de la structure génétique sur la qualité des prédictions a été évalué au sein du premier panel pour des caractères de productivité et de phénologie. Cette étude a mis en évidence l'intérêt d'une population d'entraînement (TS) dont la constitution en matière de groupes génétiques est similaire à celle de la population à prédire. Assembler les différents groupes au sein d'un TS multi-groupe apparaît comme une solution efficace pour prédire un large spectre de diversité génétique. Des indicateurs a priori de la précision des prédictions génomiques, basés sur le coefficient de détermination, ont également été évalués, mettant en évidence une efficacité variable selon le groupe et le caractère étudié.Une nouvelle méthodologie GWAS a ensuite été développée pour étudier l'hétérogénéité des effets capturés par les SNPs selon les groupes. L'intégration des individus admixés à l'analyse permet de séparer les effets des facteurs responsables de l'hétérogénéité des effets alléliques: différence génomique locale (liée au LD ou à une mutation spécifique d'un groupe) ou interactions épistatiques entre le QTL et le fonds génétique. Cette méthodologie a été appliquée au panel “Flint-Dent” pour la précocité de floraison. Des QTL ont été détéctés comme présentant des effets groupe-spécifiques interagissant ou non avec le fonds génétique. De nombreux QTL présentant un profil original ont pu être mis en évidence, incluant des locus connus tels que Vgt1, Vgt2 ou Vgt3. Une importante épistasie directionnelle a aussi été mise en évidence grâce aux individus admixés, confortant l'existence d'interactions épistatiques avec le fonds génétique pour ce caractère.Sachant l'existence de cette hétérogénéité d’effets alléliques, nous avons développé deux modèles de prédictions génomiques nommées Multi-group Admixed GBLUP (MAGBLUP). Ceux-ci modélisent des effets groupe-spécifiques aux QTLs et sont adaptés à la prédiction d'individus admixés. Le premier permet d'identifier la variance génétique additionnelle créée par l'admixture (variance de ségrégation), alors que le second permet d'évaluer le degré de conservation des effets alléliques entre groupes. Ces deux modèles ont montré un intérêt certain par rapport à des modèles standards pour prédire des caractères simulés, mais plus limité sur des caractères réels.Enfin, l'intérêt des individus admixés dans la constitution de TS multi-groupes a été évalué à l'aide du second panel. Si leur intérêt a clairement été mis en évidence pour des caractères simulés, des résultats plus variables ont été observés avec les caractères réels, pouvant s'expliquer par la présence d'interactions avec le fonds génétique.Les nouvelles méthodes et l'utilisation d'individus admixés ouvrent des pistes de recherches intéressantes pour les études de génétique quantitative en population structurée. / The advent of molecular markers (SNPs) has revolutionized quantitative genetics methods by enabling the identification of regions involved in the genetic determinism of traits (QTLs) thanks to association studies (GWAS), or the prediction of the performance of individuals using genomic information (GS). The stratification of populations into genetic groups is common in animal and plant breeding. This structure can impact GWAS and GS methods through group differences in QTL allele frequencies and effects, as well as in linkage disequilibrium (LD) between SNP and QTL.During this thesis, two maize diversity panels were used, presenting different levels of structuration: the "Amaizing Dent" panel representing the diversity of dent lines used in Europe and the "Flint-Dent" panel including dent, flint and admixed lines between these two groups.In GS, the impact of genetic structure on genomic prediction accuracy was evaluated in the first panel for productivity and phenology traits. This study highlighted the interest of a training population (TS) whose constitution in terms of genetic groups is similar to that of the population to be predicted. Assembling the different groups within a multi-group TS appears as an effective solution to predict a broad spectrum of genetic diversity. A priori indicators of genomic prediction accuracy, based on the coefficient of determination, were also evaluated and highlighted a variable efficiency depending on the group and the trait.A new GWAS methodology was then developed to study the heterogeneity of the allele effects captured by SNPs depending on the group. The integration of admixed individuals to such analyses allows to disentangle the factors causing the heterogeneity of allele effects across groups: local genomic difference (related to LD or group-specific mutation) or epistatic interactions between the QTL and the genetic background. This methodology was applied to the "Flint-Dent" panel for flowering time. QTLs have been detected as presenting group-specific effects interacting or not with the genetic background. QTLs with an original profile have been highlighted, including known loci such as Vgt1, Vgt2 or Vgt3. Significant directional epistasis has also been demonstrated using admixed individuals and supported the existence of epistatic interactions with the genetic background for this trait.Based on the existence of such heterogeneity of allele effects, we have developed two genomic prediction models named Multi-group Admixed GBLUP (MAGBLUP). Both model group-specific QTL effects and are suited to the prediction of admixed individuals. The first allows the identification the additional genetic variance created by the admixture (segregation variance), while the second allows the evaluations of the degree of conservation of SNP allele effects across groups. These two models showed a certain interest compared to standard models to predict simulated traits, but it was more limited on real traits.Finally, the interest of admixed individuals in multi-group TS was evaluated using the second panel. Although their interest has been clearly demonstrated for simulated traits, more variable results have been observed with the real traits, which can be explained by the presence of interactions with the genetic background.The new methods and the use of admixed individuals open interesting lines of research for quantitative genetics studies in structured population.

In the world of software development, there is an increasing demand for software to keep up with rapid changes in its real-world context. A Resilient Digital Twin of an Organization is a type of software whose purpose is to digitally represent an organization or a component of an organization - as a Digital Twin -, and to keep doing so accurately throughout the real-world organization’s changes - a Resilient Digital Twin. An architectural pattern, called the EA Blueprint Pattern, has recently been proposed as a pattern to use for developing Resilient Digital Twins that can change together with the changes in the organization. However, software architecture is not the only factor that enables continuous change and adaptability in software. For software development teams to be able to deliver software rapidly and reliably, the software development process itself must be adapted to allow for frequent and fast changes. From this need, the Agile methodology and subsequently the set of work practises called DevOps has emerged. DevOps leverages automation and fast feedback as tools to facilitate a shorter system development life cycle and continuous delivery. The usage of DevOps is becoming increasingly popular in the software development field. It stands to reason that there is a need to ensure that the EA Blueprint Pattern is appropriate even in a DevOps context, where different tools and routines may be used than in traditional development. To complete this project, a use case of the EA Blueprint Pattern has been moved from a traditionally developed and deployed setting to a DevOps setting that includes essential DevOps tools such as Infrastructure as Code, a cloud environment, and a CI/CD pipeline that enables automatic deployment and therefore a shorter system development life cycle. By doing this, it can be gauged how well the EA Blueprint Pattern is adapted to a modern software development process which utilises the advantages of DevOps.