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Transport cellobiose médié par PTS et son effet sur l'expression du gène de virulence chez Listeria monocytogenes / PTS-mediated cellobiose transport and its effect on virulence gene expression in Listeria monocytogenesCao, Minh Thanh Nguyen 17 December 2015 (has links)
Listeria monocytogenes transporte le cellobiose principalement via le PTS (PEP:carbohydrate phosphotransferase system). La croissance sur cellobiose induit l'expression des opérons celBCA1, celBA2 ainsi que du gène lmrg_01989, qui codent respectivement le composant soluble EIIACel1, le transporteur EIICCel1, le composant soluble EIIBCel1, les protéines EIIBCel2 et EIIACel2, et une seconde EIICCel. La croissance sur glucose réprime fortement l'expression de ces gènes. La délétion de celC1 codant l'EIICCel1 ou des deux gènes, celA1 et celA2, ralentit considérablement la consommation cellobiose. L'expression des trois unités de transcription induite par le cellobiose dépend de CelR. CelR, qui code un régulateur transcriptionnel LevR- like, est situé en aval de l'opéron bicistronique celBA2. CelR est activé par phosphorylation par EI et HPr de l'His550. En revanche, la phosphorylation de l'His823, catalysée par P~EIIBCel1 et P~EIIBCel2, inhibe l'activité de CelR. Le remplacement de l'His823 par une Ala empêchant cette phosphorylation ou la délétion des deux gènes codants les EIIAsCel ou EIIBsCel entraîne l'expression constitutive des trois unités de transcription contrôlées par CelR. Comme le glucose, le cellobiose inhibe fortement l'activité de PrfA, l'activateur des gènes de virulence. Nous avons donc cherché à tester si l'un des composants PTSCel pouvait être impliqué dans la répression de gènes de virulence. Les mutants consommant faiblement le cellobiose, présentaient une levée de la répression des gènes de virulence par le cellobiose, alors que le glucose et les autres sucres-PTS les réprimaient toujours. De manière surprenante, la délétion du gène monocistronique lmrg_00557, qui code un autre composant EIIBCel du PTS, induisait la levée de la répression des gènes de virulence médiée par toutes les sources de carbone mais n'avait aucun effet sur la consommation de glucose ou de cellobiose. Ce gène lmrg_00557 a été appelé vgiB (virulence gene inhibitor B) et la protéine correspondante, qui semble jouer un rôle majeur dans la régulation de l'activité de PrfA, EIIBVir. Cette protéine est phosphorylée par le PEP et les composants PTS EI, HPr et EIIACel2 sur le résidu cystéine-8. La complémentation du mutant ΔvgiB avec l'allèle sauvage, mais également avec l'allèle Cys8Ala, restaurait le mécanisme général de répression des gènes de virulence par les sucres, suggérant ainsi que la forme non phosphorylée de EIIBVir inhibe l'activité de PrfA. / Listeria monocytogenes transports cellobiose mainly via a PEP:carbohydrate phosphotranseferase system (PTS). Growth on cellobiose induces the expression of the celBCA1 and celBA2 operons as well as lmrG01989, which encode the soluble EIIA Cel1 and EIIB Cel1 components, the transporter EIIC Cel1 , the EIIA Cel2 and EIIB Cel2 proteins, and a second EIIC Cel , respectively. Growth on lucose strongly repressed the expression of these genes. Deletion of the EIIC Cel1 –encoding celC1 or of both, celA1 and celA2, significantly slowed cellobiose consumption. The bicistronic operon celBA2 is located downstream from celR, which codes for a LevR-like transcription activator. Expression of the three cellobiose-induced transcription units depends on CelR. The gene encoding CelR is located upstream from the bicistronic operon celBA2. CelR itself is activated via phosphorylation by EI and HPr at His550. In contrast, phosphorylation at His823, which is catalyzed by both, P~EIIB Cel1 and P~EIIB Cel2 , inhibits CelR activity. Preventing this phosphorylation by replacing His823 with Ala or deleting the two EIIA Cel – or EIIB Cel -encoding genes caused constitutive expression of all three CelR-controlled transcription units. Similar to glucose, cellobiose strongly inhibits the activity of the virulence gene activator PrfA. We therefore tested whether one of the PTS Cel components might be involved in virulence gene repression. Mutants, that exhibit slow cellobiose consumption, were relieved from cellobiose-mediated virulence gene repression, whereas glucose and other PTS-sugars still repressed them. Strikingly, deletion of the presumed monocistronic lmrg_00557, which codes for another EIIB Cel -like PTS component, caused a general relief from carbon source-mediated virulence gene repression, but had no effect on cellobiose or glucose consumption. The gene lmrg_00557 was named vgiB (virulence gene inhibitor B) and the encoded protein, which seems to play a major role in PrfA regulation, was called EIIB Vir . It becomes phosphorylated by PEP and the PTS components enzyme I, HPr and EIIA Cel2 at cysteine-8. Complementation of the ΔvgiB mutant with wild-type vgiB, but also with the Cys8Ala allele restored general virulence gene repression, thus suggesting that it is the unphosphorylated form of EIIB Vir , which inhibits the activity of PrfA.
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Hydrolytic and Oxidative Mechanisms Involved in Cellulose DegradationNutt, Anu January 2006 (has links)
<p>The enzymatic degradation of cellulose is an important process in nature. This thesis has focused on the degradation of cellulose by enzymes from two cellulose-degrading fungi, <i>Hypocrea jecorina</i> and <i>Phanerochaete chrysosporium</i>, including both the action of the individual enzymes and their synergistic interplay. </p><p>The end-preference of cellobiohydrolases on crystalline cellulose was studied. Cellobiohydrolases belonging to glycosyl hydrolase (GH) family 7 were found to hydrolyse cellulose processively, starting from the reducing end of the cellulose chain. End-labelled cellulose can serve as a tool for functional classification of cellulases.</p><p>The synergy mechanism between endoglucanases and cellobiohydrolases was studied using substrates with different physical properties derived from bacterial cellulose. A new mechanism for synergism between endo- and exoacting enzymes was proposed whereby endoglucanases, in addition to creating nicks in amorphous parts of cellulose, thereby making new starting-points for processively acting cellobiohydrolases, also “polish” the cellulose surface by removing shorter chains from cellulose surface.</p><p>A new small endoglucanase belonging to the GH12 family was isolated and characterised. The proposed role of this enzyme is to make the cellulose in wood more accessible to other cellulases.</p><p>Oxygen conversion by cellobiose dehydrogenase was studied. Hydrogen peroxide produced by cellobiose dehydrogenase can be decomposed even by traces of certain metal ions into a hydroxyl radical and a hydroxyl ion. As an example, reduced metal ions will be continuously regenerated by cellobiose dehydrogenase, which thus stimulates the degradation.</p><p>Interactions between GH7 family cellobiohydrolases and o-nitrophenyl cellobioside were studied by fluorescence spectroscopy and kinetic tests. o-nitrophenyl cellobioside was used as indicator ligand to determine the dissociation constants for cellobiose binding to catalytically inactive Cel7A mutants by displacement binding experiments.</p>
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Hydrolytic and Oxidative Mechanisms Involved in Cellulose DegradationNutt, Anu January 2006 (has links)
The enzymatic degradation of cellulose is an important process in nature. This thesis has focused on the degradation of cellulose by enzymes from two cellulose-degrading fungi, Hypocrea jecorina and Phanerochaete chrysosporium, including both the action of the individual enzymes and their synergistic interplay. The end-preference of cellobiohydrolases on crystalline cellulose was studied. Cellobiohydrolases belonging to glycosyl hydrolase (GH) family 7 were found to hydrolyse cellulose processively, starting from the reducing end of the cellulose chain. End-labelled cellulose can serve as a tool for functional classification of cellulases. The synergy mechanism between endoglucanases and cellobiohydrolases was studied using substrates with different physical properties derived from bacterial cellulose. A new mechanism for synergism between endo- and exoacting enzymes was proposed whereby endoglucanases, in addition to creating nicks in amorphous parts of cellulose, thereby making new starting-points for processively acting cellobiohydrolases, also “polish” the cellulose surface by removing shorter chains from cellulose surface. A new small endoglucanase belonging to the GH12 family was isolated and characterised. The proposed role of this enzyme is to make the cellulose in wood more accessible to other cellulases. Oxygen conversion by cellobiose dehydrogenase was studied. Hydrogen peroxide produced by cellobiose dehydrogenase can be decomposed even by traces of certain metal ions into a hydroxyl radical and a hydroxyl ion. As an example, reduced metal ions will be continuously regenerated by cellobiose dehydrogenase, which thus stimulates the degradation. Interactions between GH7 family cellobiohydrolases and o-nitrophenyl cellobioside were studied by fluorescence spectroscopy and kinetic tests. o-nitrophenyl cellobioside was used as indicator ligand to determine the dissociation constants for cellobiose binding to catalytically inactive Cel7A mutants by displacement binding experiments.
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The role of acid in the cerium (IV) oxidation of carbohydratesCzappa, Dennis J. 01 January 1974 (has links)
No description available.
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The hydrolysis of cellobiose in the presence of ferric ionKraske, Karl V. 06 1900 (has links)
No description available.
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Etude des conditions physiologiques influançant [i.e. influençant] la croissance, le catabolisme du cellubiose et la sporulation de Clostridium Cellulolyticum ATCC 35319Payot-Lacroix, Sophie. Petitdemange, Henri January 1999 (has links) (PDF)
Thèse doctorat : Sciences biologiques fondamentales et appliquées. Psychologie : Nancy 1 : 1999. / Titre provenant de l'écran-titre.
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Model Analysis of Cellobiose Solubility in Organic Solvents and WaterHeng, Joseph O. 18 May 2020 (has links)
The solubility of cellobiose in 18 organic liquids and water was measured at 20°C. Hydrogen bond acceptors were the most effective solvents. Three models were analyzed to evaluate their accuracy and to understand factors that affect cellobiose solubility: Hansen solubility parameters (HSP), linear free energy relationship (LFER), and UNIQUAC functional-group activity coefficients (UNIFAC). The HSP of cellobiose were determined and the model was able to distinguish between most good and poor solvents, however, proved to be occasionally unreliable due to a false negative. The LFER model produced an empirical equation involving contributions from solvent molar refraction, polarizability, acidity, basicity, and molar volume, which predicted cellobiose solubilities to within ±2 log units. LFER indicated that good solvents were highly polarizable and had low molar volume, which was consistent with the good solvents found for cellobiose. A modified version of UNIFAC that includes an association term (A-UNIFAC) predicted the solubility of cellobiose in water and alcohols to within ±0.6 log units, indicating that A-UNIFAC can be used to predict the solubility of cellobiose and other carbohydrates provided additional data to extend the model to solvents other than water and alcohols.
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Cellobiose dehydrogenase from Clonostachys rosea: Production, purification and activity analysisLarsson, Terese January 2021 (has links)
Biological control agents are a promising niche to replace chemical pesticides for treating plant pathogens in agriculture. A potential biocontrol agent is the microparasitic fungi Clonostachys rosea which has the ability to attack various plant pathogens such as other fungi and nematodes. One key feature in the interaction between mycoparasite and prey is degradation of the fungal cell wall where cell wall degrading enzymes are important. One cell wall degrading enzyme is cellobiose dehydrogenase of which it has been found a high number of genes for in C. rosea compared to other mycoparasites. The reason for these many cellobiose dehydrogenase genes being present in C. rosea is what this study aimed to find out. To do so, the different cellobiose dehydrogenase proteins 001, 002, 003 and 004 were successfully expressed in Pichia pastoris. The 003 protein had significantly higher expression levels and were further purified with size exclusion chromatography where some of the resulting purified protein was used to set up a crystallization screen. Unfortunately, no crystals have been formed so far. The enzymatic activity against lactose, cellobiose and laminaribiose of all produced cellobiose dehydrogenase proteins were also analyzed using a 2,6-dichloroindophenol activity assay. The proteins 001 and 002 showed a low activity against lactose and cellobiose whereas the other protein showed no activity for the tested conditions. That these proteins have developed variations in their activities may be one reason for why they are all still existing.
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Matériaux à porosité contrôlée sulfonés : Synthèse, Caractérisation, Etude des propriétés catalytiques / Sulfonated ordered mesoporous materials : Synthesis, Charcacterization, Catalytic propertiesKaraki, Mariam 08 July 2013 (has links)
La catalyse solide acide a été pendant longtemps l'objet d'activité de recherche intense, en particulier pour l'industrie pétrochimique. Aujourd'hui, les catalyseurs solides acides sont de plus en plus étudiés dans d'autres domaines et en particulier dans celles liées à la «chimie verte» et à la valorisation des bioressources, telles que la synthèse de biodiesel et la transformation des polysaccharides. L’objectif de la thèse est d’étudier le potentiel des matériaux poreux sulfonés ayant une porosité contrôlée dans des réactions catalysées par un acide en condition eau surchauffé telle que l'hydrolyse de la cellobiose. Dans une première partie, nous décrivons la préparation et la caractérisation des organosilicates mésoporeux périodiques sulfonés de type SBA-15, SBA-1 et KIT-6 par co-condensation de 1,4-bis (triéthoxysilyl) benzène (BTEB). Les matériaux ont été acidifiés suivant des voies différentes à l'aide de 3-mercaptopropyltriméthoxysilane (MPTMS)/H2O2 ou d'acide chlorosulfonique (ClSO3H). Leur propriété acide a été étudiée par adsorption d’NH3 suivie par calorimétrie et par la réaction de déshydratation d'isopropanol (IPA) comme réaction modèle en phase gazeuse. Contrairement à notre attente, l'adsorption d’NH3 suivie par calorimétrie a mis en évidence l'hétérogénéité de la force des sites suggérant la présence de sites distincts de la sulfonation. Les solides sulfonés avec l'acide chlorosulfonique ont une activité équivalente à celle de la résine sulfonée, Amberlyst 15, mais ils sont moins stables en raison de la libération des espèces de soufre. Les catalyseurs préparés en utilisant un groupement mercapto-propyle suivie d’une oxydation sont moins acides et ils ont donné des niveaux d'activité plus basse dans la réaction de déshydratation d'IPA. Pour l'hydrolyse de la cellobiose, de bonnes performances ont été obtenues à 150°C, mais, ces matériaux se sont montrés instables dans des conditions hydrothermales avec une lixiviation totale de soufre réalisant alors la réaction en phase homogène. Un lavage dans l'eau surchauffée des matériaux contenant des groupements propyles-SO3H conduit à une diminution de leur efficacité dans l'hydrolyse de la cellobiose, mais un gain de stabilité a été obtenu, permettant le recyclage de ces matériaux. Dans une deuxième partie, des répliques carbonées sulfonées par l’acide chlorosulfonique ou l’acide sulfurique ont été synthétisé. La sulfonation par l’acide sulfurique suivi par un lavage dans l’eau bouillante puis un prétraitement thermique à 300°C sous azote, de ces matériaux aboutissent au meilleur catalyseur en termes d’activité/stabilité. / Catalysis with solid acids has been for a long time the subject of intense research activities, especially for the petrochemical industry. Nowadays, solid acid catalysts are more and more studied in other areas and particularly in those related to “green chemistry” and bioresources valorization such as biodiesel synthesis and now polysaccharides transformations. The present work aimed to investigate the potential of acidic ordered mesoporous materials with a controlled local environment of the acid sites for applications in acid catalyzed reactions in hot water such as cellobiose hydrolysis. First we described the synthesis of periodic mesoporous organosilicas SBA-15, SBA-1 and KIT-6 types, from the condensation of 1,4-Bis(triethoxysilyl)benzene. The material was sulfonated using 3-mercaptopropyltrimethoxysilane further oxidized with H2O2 or chlorosulfonic acid to give Brønsted solid acids which were fully characterised. Their acidic properties were studied by calorimetry of NH3 adsorption and in the model reaction of gas phase isopropanol dehydration. The calorimetry of NH3 adsorption has evidenced the heterogeneity of the acid strength distribution suggesting the presence of distinct sites of sulfonation contrary to our expectation. For gas phase isopropanol (IPA) dehydration, the solids sulfonated with the chlorosulfonic acid exhibited an activity equivalent to that of the sulfonated resin, Amberlyst 15, but were less stable due to sulphur species release, assumed to be sulfonated silanols. The acidic organosilicas obtained via H2O2 oxidation of the mercapto-propyl group are less acidic catalysts, showing a low activity for gas phase IPA dehydration. In the hydrolysis reaction, the solids were active at 150 °C however sulfur leaching analysis showed that the reaction preceded mainly homogeneously, especially for the material acidified with chlorosulfonic acid. A hot washing pre-treatment applied to the catalysts containing the sulfonated propyl groups, led to a decrease of their hydrolysis activity but along with a gain of stability allowing recycling. Second we described the synthesis of ordered mesoporous carbon and their sulfonation with chlorosulfonic acid or sulfuric acid. Sulfonation of carbon replicas with sulfuric acid followed by washing in hot water and thermal pretreatment at 300°C under nitrogen, lead to the best catalyst in terms of activity / stability.
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Nouvelles enzymes fongiques pour l'amélioration de la dégradation de la biomasse lignocellulosique : étude des "Lytic Polysaccharide Monooxygenases" (LPMOs) / New fungal enzymes for the improvement of lignocellulosic biomass degradation : study of the "Lytic Polysaccharide Monooxygenases" (LPMOs)Bennati-Granier, Chloe 02 February 2016 (has links)
Dans le contexte actuel, il devient nécessaire de rendre les alternatives au pétrole, tel que le bioéthanol 2G, disponibles à grande échelle. Cependant, l’étape d’hydrolyse par les enzymes de Trichoderma reesei reste un verrou à un procédé économiquement stable et rentable. Ces travaux de thèse, s'intègrent dans le cadre du projet Futurol et ont pour objectifs d'identifier et de caractériser de nouvelles enzymes fongiques pour améliorer l'hydrolyse de la biomasse lignocellulosique. A partir des données protéomiques disponibles pour Podospora anserina et Fusarium verticillioides, une douzaine d'enzymes candidates ont été identifiées dans leurs sécrétomes. Ce travail de thèse s'est plus particulièrement focalisé sur les AA9s « Lytic Polysaccharide Monooxygenases » (LPMOs) de P. anserina. Parmi les LPMOs étudiées, PaLPMO9A, PaLPMO9E et PaLPMO9H, qui possèdent un CBM1, sont les plus actives sur la cellulose. La détermination de la régiosélectivité d'action a mis en évidence que PaLPMO9A et PaLPMO9H clivent la cellulose en position C1 et C4 alors que la PaLPMO9E génère uniquement des produits oxydés en C1. La PaLPMO9H est la plus versatile puisqu’elle est active sur les cello-oligosaccharides solubles et sur les polysaccharides hémicellulosiques liés en β-(1,4) (i.e., xyloglucane, glucomannane). La supplémentation du cocktail de T. reesei avec PaLPMO9E ou PaLPMO9H a permis de doubler les rendements d'hydrolyse du miscanthus prétraité. Les travaux réalisés au cours de cette thèse ont permis de démontrer l'importance de ces enzymes oxydatives dans les phénomènes de déconstruction de la lignocellulose chez les champignons filamenteux. / In the current context, it becomes essential to make alternative to oil, such as the 2G bioethanol, available at large scale. However, the hydrolysis step by Trichoderma reesei enzymes remains the major bottleneck for an economically sustainable process. The present work is part of the Futurol project, and aims at identifying and characterizing new fungal enzymes to improve the hydrolysis of lignocellulosic biomass. From the proteomic data available for Podospora anserina and Fusarium verticillioides, a dozen of interesting enzymes were identified in their secretomes. This work focuses, mainly, on the AA9s « Lytic Polysaccharide Monooxygenases » (LPMOs) from P. anserina. Among all the LPMOs studied, PaLPMO9A, PaLPMO9E and PaLPMO9H that harbored a CBM1 were the most active on cellulose. Investigation of their regioselective mode of action revealed that PaLPMO9A and PaLPMO9H oxidatively cleaved at both C1 and C4 positions while PaLPMO9E released only C1-oxidized products. PaLPMO9H that was the most versatile in terms of substrate specificity as it also displayed activity on cello-oligosaccharides and β-(1,4)-linked hemicellulose polysaccharides (e.g., xyloglucan, glucomannan). The hydrolysis yield of the pretreated miscanthus was significantly improved up to 2 fold, when the PaLPMO9E, or PaLPMO9H were supplemented to the T. reesei cocktail. This work demonstrated the importance of these oxidative enzymes for lignocellulose deconstruction by fungi. These biocatalysts open new prospects to improve the enzymatic conversion of plant biomass for 2G bioethanol production.
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