The enzyme inosine triphosphate pyrophosphatase (ITPase) is responsible for controlling the levels of the by-products guanosine monophosphate (GMP) and adenosine monophosphate (AMP) through their precursor inosine monophosphate (IMP). ). Human ITPase consists of a 194-amino acid homodimer which relies upon either an Mg2+ ion or a Mn2+ ion for catalytic activity, and orthologs of this protein have been found in many different organisms. The purpose of this project was to try out methods learned throughout the education and to use this knowledge to gather new data about the human protein inosine triphosphate pyrophosphatase (ITPase). The protein was expressed in BL21/DE3 cells from a pre-made vector. Experiments performed during this project include secondary- and tertiary stability measurements, tryptophan fluorescence spectra, binding curve and thermic stability to ITPase with ANS and methotrexate. The Tm-value of human ITPase was examined with Trp-Fluorescence, ANS-fluorescence and Near-UV and Far-UV circular dichroism (CD). The stability of ITPase monitored by Near-UV as well as Far-UV coincides, indicating that secondary- and tertiary-unfolding occur simultaneously without any intermediates. The results of Trp-fluorescence showed that the tryptophans were already exposed and thus it did not yield a reliable result. The binding properties of ANS and MTX to ITPase were also examined.

Single frame ultrasound B-mode image quality is largely governed by the ability to focus the ultrasound beam over a range in depths both in transmission and reception. By developing a comprehensive understanding of acoustic wave propagation two signal processing methods were identified for solving the transmission problem. We made use of both the impulse response using the classical point spread function (PSF) and the spatial sensitivity function (SSF) which describes the spatial distribution at a particular time. Using the angular spectrum method, an accurate analytical model was developed for the field distribution arising from a finite geometry, apodized and focused, plane piston transducer. While there is a thorough understanding of the radiated field arising from uniformly excited plane piston transducers, the focused equivalent (i.e., one that allows a continuous change in phase over the plane piston surface) is incomplete and assumes the Fresnel approximation. Our model addresses the effects of diffraction and evanescent waves without the use of the Fresnel approximation and is applicable at all near- and far-field locations in a lossless medium. The model was analyzed to identify new insights into wave propagation and compared with the Fresnel approximation and the spherically-focused, concave transducer. The piston transducer model was then extended to an attenuating and dispersive medium. After analysing existing models of power-law frequency dependent attenuation, a causal, spherical wave Green’s function was derived from the Navier-Stokes equation for a classical viscous medium. Modifications to the angular spectrum method were presented and used to analyze the radiated field of a focused, planar piston transducer. Finally, after presenting our signal processing strategy for improving imaging spatial resolution through minimization of the SSF, two signal processing methods were derived and analysed in simulation: a deconvolution technique to remove the effects of the ultrasound excitation wave and suppress additive noise from the received ultrasound signal, and a retrospective transmit focusing method that changed the response from a predefined transmit focus to an arbitrary transmit focal depth. Proof-of-concept simulations were presented using a variable number of scatterers and compared with the traditional matched filtering and envelope detection technique.

A large acceptance polarimeter has been designed to measure recoil polarisation in pseudoscalar (Jπ=0−) meson photoproduction reactions. The device was installed at the MAMI facility at the Institut für Kernphysik in Mainz, Germany. A racetrack microtron provided a longitudinally polarised 1.5 GeV electron beam, which impinged on a 12 μm copper radiator, producing a beam of circularly polarised Bremsstrahlung photons with energies between 400-1400 MeV. The electrons were then momentum analysed in the Glasgow Photon Tagging Spectrometer to tag the photon energy with a resolution of ~4 MeV. The photons were incident on a liquid hydrogen target, and the reaction products were detected using the Crystal Ball and TAPS detectors. The beam-recoil polarisation observable Cx, which describes the fraction of circular polarisation transferred from the photon beam to the recoiling nucleon, was measured in the reactions γp→pπ0 and γp→pη from data taken in September-October 2008. The results for π0 production give a significant expansion of the world data set and are shown to be consistent with the few previous measurements taken at Jefferson Lab, USA, while the results for the η channel are a world first. The observed values for Cx are compared to the current solutions from the two leading partial wave analyses, SAID and MAID, with wide angular coverage up to a photon beam energy of 1400 MeV. Significant discrepancies in the prediction of Cx can be resolved by the new data.

Rectangular collimation is used in dentistry to reduce radiation by restricting the x-ray beam to approximately the size of a number 2 intraoral film (3.2X4.1 cm). However, this restricted beam size can lead to exposure errors. The aim of this study was to retrospectively evaluate the number of radiographs exposed and the presence of technical errors by the use of traditional circular or rectangular collimators during endodontic therapy on simulated teeth in manikins. A total of 1475 digital radiographs of 84 teeth exposed by 60 dental students were evaluated. Evaluation was done by a board certified endodontist, an endodontic resident, and a dental student. Analysis of the different raters showed no significant differences among the three. Radiographs were randomized and blindly renamed. Repeated-measures mixed-model ANOVA was used to compare the number of radiographs exposed using the different collimators. Although not statistically significant (P<.05), there were 15% more radiographs taken with the rectangular collimator when compared to the circular collimator. Using a repeated-measures logistic regression, there was a significant difference of the proportion of radiographs with cone cuts (P = .0003) taken with a rectangular collimator (59%) compared to radiographs taken with a circular collimator (19%). There was no significant evidence for a collimator difference when considering missed apex (P = .0986) or missed apex due to a cone cut (P = .0631). In order to expose high quality radiographs avoiding cone cuts, a traditional circular collimator may be indicated for use during endodontic therapy.

In the present work the vacuum polarization and the circular dichroism of hydrogen-like atoms are studied. We derive equations for the Fourier transforma- tion of the vacuum expectation value of the charge density. We use it to derive Uehling potential and calculate energy shifts caused by it. Then we discuss effects of vacuum polarization in higher orders of α. In second part we define circular dichroism and we express it in terms of reduced matrix elements. Then we derive the formula for parity vilating potential which is generated by weak interaction and together with other results we use it to find the expression for circular dichro- ism in terms of hydrogen radial functions. 1

Two compact planar crossed-dipole antennas loaded with near-field resonant parasitic (NFRP) elements are reported. The NFRP and crossed-dipole elements are designed for the desired circularly polarized (CP) radiation. By placing the NFRP element over the driven element at angles of 0 degrees and 45 degrees, respectively, dual-band and broadband CP antennas are realized. All radiating elements of antennas are 35 mm x 35 mm x 0.508 mm (0.187 lambda(0) x 0.187 lambda(0) x 0.0027 lambda(0) at 1.6 GHz) in size. The dual-band CP antenna has a measured vertical bar S-11 vertical bar < -10-dB bandwidth of 226 MHz (1.473-1.699 GHz) and measured 3-dB axial ratio (AR) bandwidths of 12 MHz (1.530-1.542 GHz) and 35 MHz (1.580-1.615 GHz) with minimum AR CP frequencies of 1.535 GHz (AR = 0.26 dB) and 1.595 GHz (AR = 2.08 dB), respectively. The broadband CP antenna has a measured vertical bar S-11 vertical bar< -10-dB bandwidth of 218 MHz (1.491-1.709 GHz) and a 3-dB AR bandwidth of 145 MHz (1.490-1.635 GHz). These compact antennas yield bidirectional electromagnetic fields with high radiation efficiency across their operational bandwidths.

Master of Science / Department of Computing and Information Sciences / Daniel Andresen / The purpose of this project is to analyze the growth of plants under certain lighting conditions. In order to ensure ideal lighting for all plants under demanding conditions like lack of optimal light due to shadowing, side wall reflections, overlapping of plants, etc., pots are rotated manually in an irregular fashion. To keep track of the position of these plants from time to time, a marking system is used for each tray of 16 plants. These markers are unique for each tray High definition surveillance cameras placed above these plants capture the plant images periodically. These images undergo image processing. Image processing should be able to identify and recognize the plants from the identification markers that were placed within each tray and thereby draw the statistics about the growth of the plants. Hence the computing part of this project is all about extracting the identity of a plant through image processing. Image processing involves object and color recognition. Fiji, an image processing tool, is used for object recognition and the Python image module called “Image” is used for color recognition. Object recognition accurately locates the position of these circular objects and measures their size and shape. Color recognition identifies the pixel values of these circular objects. Finally the code corresponding to three-element groups of these circular units is fetched and stored. This code gives the identity of the tray and, therefore, each plant. The timestamp that is stored with each plant image along with the code fetched through image processing is used to track the location of a plant in the plant chamber through time.

This thesis is focused on optimizing the routes which are implemented in our country at night. Goods are transporting between designated central cities. It deals with creating cyclic routs, along which the goods should be effectively transported, with the respect of the cost. The instruction how to create these paths represents a heuristic method for generating cyclic paths. The algorithm uses the results provided by model that is based on a search for multiple product chart. The chart contains the minimum number of vehicles that provide transport and individual amount of transported goods. The principle of this heuristic method is to create cyclic paths in such a way to be able to serve all transportation requirements with the lowest number of reloads. This approach leads to the fact that the direct paths are preferred.

Chronic hepatitis B virus (HBV) infection is endemic to sub-Saharan Africa, and despite the availability of anti-viral agents, there is currently no cure. This double stranded DNA virus is hepatotropic, and active viral replication results in two genomic equivalents, the relaxed circular DNA (rcDNA) and covalently closed circular DNA (cccDNA). The virion encapsulated rcDNA contains a partially synthesised positive DNA stand and a gap region within the negative strand. After infection of hepatocytes, the rcDNA is repaired in the nucleus to form cccDNA. An important objective of HBV therapy is the elimination of cccDNA, as its persistence within hepatocytes has been attributed to chronic HBV infection. Therefore a reliable assay for this replication intermediate is crucial. The objective of this study was to develop a method based on real-time PCR to detect and quantify HBV cccDNA. PCR primers which flank the rcDNA gap were designed to amplify cccDNA whilst primers flanking the pre-S1 region quantify total HBV DNA. Viral DNA was extracted from HepG2.2.15 cells, along with serum and livers from HBV transgenic mice. According to this assay, cccDNA was readily detectable in transgenic mouse livers, but was present at low concentrations in serum samples. The intrahepatic HBV DNA profile of transgenic mice was found to be 40% cccDNA to 60% rcDNA. In HepG2.2.15 cells, only 2% of HBV DNA was cccDNA whilst the majority was in the form of rcDNA. These results were validated using non-radioactive Southern blothybridisation. Additionally, it was established that although RNAi-based effecters inhibit HBV replication, established cccDNA pools were not eliminated. Real-time PCR provides a convenient platform for HBV cccDNA detection as it allows for the rapid simultaneous amplification and quantification of a specific DNA target through either non-specific or specific DNA detection chemistries. In conclusion, this HBV qPCR assay should enable improved monitoring of patients’ responses to antiviral therapy

Ce travail expérimental s’intéresse à l’écoulement en aval d’un swirler statique court. L’induction d’un mouvement de swirl est une solution connue pour augmenter le gradient pariétal de vitesse. L’augmentation du frottement pariétal présente un intérêt industriel dans les échangeurs de chaleur pour accroître leurs potentiels de transfert de chaleur et diminuer leurs vitesses d’encrassement. Nous proposons d’approfondir la connaissance des écoulements avec swirl décroissant à faibles nombres de Reynolds pour lesquels l’intérêt énergétique est a priori plus important. Dans un premier temps, le champ d’écoulement est caractérisé à l’aide de méthodes optiques, pour identifier le type de tourbillon caractérisant le swirl. Dans un second temps, la méthode électrochimique est utilisée pour mesurer le frottement pariétal instationnaire. Par analogie, ces mesures permettent d’obtenir une première estimation du transfert de chaleur en écoulement anisotherme. / This experimental work is interested in understanding the flow downstream a short static swirler. Inducing a swirl motion is a known solution to increase the wall velocity gradient. The increase in wall shear stress is useful in industrial heat exchangers in order to enhance their heat transfer capabilities and reduce their fouling rates. We try to go deeper into knowledge of decaying swirl flows, for low Reynolds number for which energy interest is greater a priori. First, we study the flow field with optical methods in order to identify type of vortex characteristics of the swirl. Second, electrochemical method is used to measure the unsteady wall shear stress. By analogy, these measurements give a first estimate of the heat transfer for the case of non-isothermal flows.