Maize genetic diversity includes an array of kernel colors (red, blue, purple) with blue concentrated in the aleurone and red primarily in the pericarp. Quality protein maize (QPM) is improved over normal maize in regards to grain concentration of the essential amino acids lysine and tryptophan but has not been widely adapted in part due to lower than conventional yields. These are minimally-utilized specialty corns when compared to the yellows and whites commonly grown. Red, blue, and purple pigments are antioxidant phytochemicals produced by the plant as secondary metabolites. Antioxidants have been linked to anti-cancer and other anti-inflammatory health benefits. QPM hybrids are desirable in developing countries where subsistent agriculture is commonly practiced and quality protein cereals are non-existent. These two diverse maize categories have been the subject of little breeding research compared to normal maize and the potential for high phenolic content as well as the characterization of these QPM hybrids has not been previously investigated. We evaluated 153 maize hybrids (84 colored, 69 QPM) across three locations. High heritability estimates were found for phenolic content (0.80), tryptophan (0.46), and endosperm opacity (0.82). It was encouraging that all three traits observed little genotype by environment (GxE) interaction across diverse environments. This proved the trait analysis procedure to be robust in detecting and separating genotypes for both total phenolic content in colored maize, and amino acids in QPM. Top combiners for phenolics were the purple maize "maize morado" and red lines, with blue, yellow and white maize performing in descending order. Within the tested hybrids, high per kernel antioxidants (measured by total phenolics) may be the answer for producing the most total phenolics, with the top hybrid yielding greater than twice the total phenolics as the top yielding yellow hybrid. The top QPM hybrid out yielded the top normal hybrid by 35 and 30% for lysine and tryptophan. Additionally, QPM endosperm opacity primarily followed an additive, mid-parent trend, with some hybrids (20%) from diverse germplasm backgrounds deviating from that trend displaying the complexity and recessive nature of multiple modifier loci. Additional agronomic and composition traits were minimally correlated with phenolics.

Freie Univ., Habil.-Schr.-1998--Berlin, 1997.

Thesis (Ph. D.)--University of Wisconsin--Madison, 1962. / Typescript. Vita. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references (leaves 190-209).

Thesis (Ph. D.)--University of Chicago, Dept of Anthropology, December 1999. / Includes bibliographical references. Also available on the Internet.

Thesis (MSD (Play Therapy)) -- University of Pretoria, 2004. / Includes bibliographical references.

Thesis (M.A.)--University of Wisconsin--Madison, 1970. / eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references.

Thesis (M.S.)--University of Wisconsin--Madison. / Typescript. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references (leaves 432-440).

Made available in DSpace on 2015-04-20T12:31:37Z (GMT). No. of bitstreams: 1 Alita Moura de Lima.pdf: 2197035 bytes, checksum: 64774d32c0950f346e45e0226627e35a (MD5) Previous issue date: 2013-10-31 / Fundação de Amparo à Pesquisa do Estado do Amazonas / Tuberculosis (TB), an infectious disease that occurs in 90% of cases in the lungs, is caused by Mycobacterium tuberculosis. Although TB has been described since antiquity, being diagnosed about 100 years and has treatment from the 40s, is currently still considered a serious public health problem, being reported annually 1.1 million deaths worldwide. Knowing that the cycle of transmission of tuberculosis involving almost exclusively the human, being one of the targets for global TB control is the diagnosis, this being vital to break the chain of transmission. The Global Tuberculosis Control Programmes use bascilloscopies (microscopic detection of bacilli)as their first choice in the diagnosis of pulmonary tuberculosis. However, carbolfucsina, the dye solution used in this method is a toxic carcinogenic compound which contaminates water and endagers human health. In order to verify the potential of coloured substances produced by fungi endophytic filamentous to replace carbolfucsina, endophytes derived from vegetables H. sucuuba , C. multijuga , P. aduncum and S. reticulata were analyized. From 75 isolates, 30 were selected with capacity to produce coloured diffusible substances in solid medium. The culture filtrates were tested in for their ability to stain smears prepared from the standard strain of Mycobacterium tuberculosis H37Rv. Analysis of the staining was performed by bright field and fluorescence microscopy. Fluorescence substances identified as proteins by the Bradford technique, present in 78% of the culture filtrates, enabled the visualization of M. tuberculosis H37Rv. When the culture filtrates were tested with other microorganisms (E. coli ATCC 25922, S. aureus ATCC 25923 and C. albicans ATCC 36232) they were found to be nonspecific for M. tuberculosis. Despite the negative result of the proposed objective, there is potential for fluorescent proteins to be used in monitoring cell, tissue and protein interactions. / A tuberculose (TB), doença infecciosa que acomete em 90% dos casos os pulmões, é causada principalmente pelo Mycobacterium tuberculosis. Embora a TB tenha sido descrita desde a antiguidade, ser diagnosticada a mais de 100 anos e possuir tratamento desde a década de 40, atualmente ainda é considerada um grave problema de saúde pública, sendo registrados anualmente 1,1 milhões de óbitos no mundo. Sabendo-se que o ciclo de transmissão da tuberculose envolve quase que exclusivamente o ser humano, um dos alvos para o controle global da doença é o diagnóstico, sendo esse vital para a quebra da cadeia de transmissão. Os Programas Mundiais de Controle da Tuberculose consideram a baciloscopia como a primeira opção para diagnóstico da Tuberculose pulmonar. No entanto, a solução corante de carbolfucsina utilizada na técnica é um composto tóxico, carcinogênico, combustível e contaminante da água. Com o objetivo de verificar o potencial de substâncias coloridas produzidas por fungos endofíticos filamentosos em substituição à carbolfucsina, foram analisados endofíticos oriundos dos vegetais Himatanthus sucuuba, Copaifera multijuga, Piper aduncum e Senna reticulata. A partir de 75 isolados, foram selecionados 30 com capacidade de produzir substâncias coloridas difusíveis em meio sólido. Os filtrados do cultivo em caldo foram testados quanto à capacidade de coloração em esfregaços preparados a partir da cepa padrão de Mycobacterium tuberculosis H37Rv. A análise da coloração foi realizada por microscopia em campo claro e em fluorescência. Constatou-se a existência de capacidade de fluorescência em 78% dos filtrados de cultivo sobre as unidades bacilares de M. tuberculosis. No entanto, quando os filtrados foram submetidos a testes de coloração em outros microrganismos, Escherichia coli ATCC 25922; S. aureus ATCC 25923 e Candida albicans ATCC 36232, constatou-se serem inespecíficos para M. tuberculosis. Em análise de caracterização química a substância responsável pela fluorescência apresentou características compatíveis com proteínas. Nesse sentido, apesar do resultado negativo ao objetivo proposto, tem-se a possibilidade da descoberta de proteínas fluorescentes que vêm sendo utilizadas como reveladores na monitoração de células, tecidos e na interação entre proteínas.

Neste trabalho, foram realizados diversos experimentos para a criação controlada de nanoestruturas superficiais produzidas por Laser pulsado de femtossegundo em Titânio e em aço inoxidável, ambos utilizados para fins cirúrgicos e industriais. Os parâmetros alterados nos experimentos foram a Fluência do laser, a taxa de sobreposição de pulsos, polarização do feixe e atmosfera de processamento. A vantagem na utilização do laser de femtossegundo para essas aplicações é a possibilidade de ser criar tais estruturas sem que haja aquecimento crítico do material, evitando assim, a criação de óxidos ou nitretos superficiais, já que esses a formação desses materiais superficiais pode afetar o desempenho industrial e cirúrgico dos materiais. Após o entendimento dos parâmetros de irradiação na formação das nanoestruturas, foram propostas três aplicações possíveis para a superfície resultante do processamento com laser: Superfícies coloridas, que podem ser utilizadas em personalização de peças; Superfícies escuras, que podem ser utilizadas na marcação e tipagem de peças; e um Micro Aquecedor, que pode ser utilizado no controle de temperaturas dentro de circuitos microfluídicos. / In this work, several experiments were carried out for the controlled creation of surface nanostructures produced by femtosecond laser pulses in Titanium and stainless steel, both used for surgical and industrial purposes. The altered parameters in the experiments were the laser Fluence, the overlap pulses rate, the beam polarization and processing atmosphere. The advantage of using femtosecond laser for such applications is the possibility of creating the nanostructures without critical heating of the material, thus avoiding the creation of surface oxides or nitrides, since the formation of such surface materials may affect the industrial and surgical materials performance. After the understanding of the irradiation parameters in the formation of the nanostructures, three possible applications were proposed for the surface resulting from the laser processing: Colored surfaces, which can be used in personalization of parts; Dark surfaces, which can be used in parts marking; and a Micro Heater, which can be used to control temperatures inside microfluidic circuits.

Magister Artium - MA / In this study the theatre as staged performance and as text was used as exploratory and discursive tools to investigate the negotiation of identities. The aim was to explore this theme by examining the responses to four popular Coloured identity-related staged performances; Marc Lottering's "Crash" and "From the Cape Flats with Love", as well as Petersen, Isaacs and Reisenhoffer's "Joe Barber" and "Suip". These works, both as performance and as text, was used to investigate the way stereotypical representations of Coloured identities are played with, subverted or negotiated in performance. / South Africa