Chitosan has proven through the years as a versatile biomaterial to be used in pharmaceutical applications. Its mucoadhesive properties as well as its ability to manipulate the tight junctions in epithelium membranes have qualified it as an effective drug carrier in controlled drug delivery systems. Microparticles or beads as they are forward called in this study have advantages over conventional drug dosage forms because of a large surface to volume ratio and have the ability to target a specific site for drug release. Indomethacin is an anti-inflammatory drug that causes gastrointestinal side effects in conventional immediate-release dosage forms. The goal is to manipulate the drug delivery vehicle to target the intestines/colon as the site for drug delivery and to minimize this side effect. Thus chitosan beads have been chosen as a drug delivery system for indomethacin in this study. Chitosan beads have been prepared through the ionotropic gelation method using tripolyphophate (TPP) as a cross-linking agent. To prepare the most effective bead to encapsulate indomethacin different formulation and system variables (pH of the TPP solution, the concentration of the TPP solution as well as the indomethacin concentration) have been evaluated according to the following parameters: morphology, drug loading capacity and swelling capability. The ideal pH of the TPP solution was determined at 8.7 and the most effective TPP and indomethacin concentration were 5% w/v and 4% w/v respectively. The chitosan concentration was kept at 3% w/v throughout the study. These concentrations were used to examine the effect of pharmaceutical excipients on the indomethacin release from chitosan beads. The effect of the different excipients namely, ExplotabⒽ(0.25% w/v), Ac-Di-SolⓀ (0.5% w/v) and Vitamin C (0.25% w/v), on the morphology, drug loading capacity, swelling capability as well as the drug release of indomethacin chitosan beads (ICB's) were also studied. The excipients were used in the individually above mentioned concentrations and in combination with each other in the same concentrations. These formulations were used in dissolution studies over a period of 6 hours in PBS pH 7.4 solutions. The indomethacin release rate increased when an excipient was added to the formulation and it dramatically increased when the excipients were added in their various combinations, compared to the formulation that did not contain excipients. / Contents: Chitosan -- Controlled drug delivery -- Indomethacin -- Inotropic gelation -- Tripolyphosphate (TPP) -- Explotab® -- Ac-Di-Sol® -- Vitamin C / Thesis (M.Sc. (Pharmaceutics))--North-West University, Potchefstroom Campus, 2007.

Recent advances in micro technologies have equipped researches with novel tools for interacting with biological molecules and cells. This thesis focuses on the design, fabrication and application of microfluidic platforms for stimuli-responsive drug delivery and the electromechanical characterization of single cells. Stimuli-responsive hydrogels are promising materials for controlled drug delivery due to their ability to respond to changes in local environmental conditions. In particular, nanohydrogel particles have been a topic of considerable interest due to their rapid response times compared to micro and macro-scale counterparts. Owing to their small size and thus low drug-loading capacity, these materials are unsuitable for prolonged drug delivery. To address this issue, stimuli-responsive implantable drug delivery micro devices by integrating microfabricated drug reservoirs with smart nano-hydrogel particles embedded composite membranes have been proposed. In one proposed glucose-responsive micro device, crosslinked glucose oxidase enables the oxidation of glucose into gluconic acid, producing a microenvironment with lower pH values to modulate the pH-responsive nanoparticles. In vitro glucose-responsive drug release profiles were characterized and normoglycemic glucose levels in diabetic rats with device implantation were also recorded. The biophysical properties of single cells have recently been demonstrated as an important indicator of disease diagnosis. Existing technologies are capable of characterizing single parameter either electrical or mechanical rapidly, but not both, which could only collect limited information for cell status evaluation. To address this issue, two microfluidic platforms capable of simultaneously characterizing both the electrical and mechanical properties of single cells based on electrodeformation and integrated impedance spectroscopy with micropipette aspiration have been proposed. In one proposed microfluidic device, a negative pressure was used to suck cells continuously through the aspiration channel with impedance profiles measured. By interpreting impedance profiles, transit time and impedance amplitude ratio can be quantified as cellular mechanical and electrical property indicators. Neural network based cell classification was conducted, demonstrating that two biophysical parameters could provide a higher cell classification success rate than using electrical or mechanical parameter alone.

This thesis evaluates the effectiveness of study components in order to aid in design refinements for a larger randomized controlled trial (RCT). A total of 38 post-secondary students with Attention-Deficit/Hyperactivity Disorder (ADHD) were randomized into a waitlist control group, or standard-length (45 minute) or shortened-length (15 minute) WM training group. Criterion measures included the WAIS-IV Digit Span (auditory-verbal WM), CANTAB Spatial Span (visual-spatial WM) and WRAML Finger Windows (visual-spatial WM). Transfer-of-training effects were assessed using indices of everyday cognitive functioning. Participants in the standard- and shortened-length groups showed greater improvements at post-test on auditory-verbal WM and reported fewer cognitive failures in everyday life than waitlist controls. Participants in the standard-length group showed greater improvements in visual-spatial WM at post-test than participants in the other two groups. Preliminary findings suggest that shorter training may have similar beneficial outcomes as documented for the standard-length training, indicating that a larger-scale RCT is warranted.

Mean daily vegetable consumption is below the recommended 7-10 servings in the diet of adult Canadians. This thesis aimed to investigate the moderating role of perceived barriers on the relationship between motivation and (greater) vegetable consumption. 606 people from the Ontario Food panel participated in the online study. The results identified a positive moderating effect of perceived barriers on the relationship between autonomous motivation and vegetable consumption (p < .05) and negative approaching significance moderating effect of perceived barriers on controlled motivation and vegetable intake (p = 0.056). Also four motivational profiles of consumers were identified and described in terms of vegetable consumption, food related behaviors, stages of change and socio-demographic characteristics. These results extend the Self-Determination Theory with perceived barriers component and can inform intervention program development for social marketers and policy makers.

Because conventional seismic force resisting systems rely on yielding of key structural members to limit seismic forces, structural damage is expected after a design-level earthquake. Repairing this damage can be very expensive, if it is possible at all. Researchers have been developing a new family of self-centring systems that avoid structural damage. One such system is a controlled rocking steel frame, which is the subject of this thesis. In a controlled rocking steel frame, the columns of a frame are permitted to uplift from the foundation, and the response is controlled by using a combination of post-tensioning and energy dissipation. Although previous studies have confirmed the viability of this system, they have also shown that rocking does not fully limit the peak seismic forces because of higher mode effects. If a structure is designed to account for these effects, it may be uneconomical, but if it is not designed to account for them, it may be unsafe. The purpose of this thesis is to develop recommendations for the design of controlled rocking steel frames, particularly with regard to higher mode effects. A theoretical framework for understanding higher mode effects is developed, and large-scale shake table testing is used to study the behaviour of a controlled rocking steel frame. Two mechanisms are proposed to mitigate the increase in structural forces due to higher mode effects, and these mechanisms are validated by shake table testing. Numerical modelling of controlled rocking steel frames is shown to become more reliable when higher mode mitigation mechanisms are used to limit the seismic response. In the final chapters, the thesis proposes and validates a new methodology for the limit states design of controlled rocking steel frames.

Significant advances have been made in the understanding of living/controlled radical polymerization processes since their discovery in the early 1990’s. These processes enable an unprecedented degree of control over polymer architecture that was previously not possible using conventional radical polymerization processes, and this has made possible the synthesis of many new and interesting materials. However, there has been only limited success in commercializing these new methods. Recently there has been increased focus on the development of more industrially viable processes. Dispersed aqueous phase reactions have received much attention because these water-based processes have several technical, economic, and environmental benefits over the more common solution and bulk reactions that were originally developed. Likewise, there has been some investigation of using continuous reactors that have potential technical and economic benefits over the more commonly employed batch reactors. This thesis presents an in-depth study that combines the three aforementioned technologies: living/controlled radical polymerization, dispersed phase aqueous reactions, and continuous reactors. Specifically, the system of interest is a nitroxide-mediated miniemulsion polymerization reaction in a continuous tubular reactor to produce polymer latex. Design of the continuous tubular reactor is discussed in some detail with a focus on specific technical challenges that were faced in building a functional apparatus for this system. Scoping experiments are described which identified a significant effect of temperature ramping rate that is critical to understand when moving to larger scale reactors for this system. The unexpected phenomenon of room temperature polymerization initiated by ascorbic acid is also described. There is demonstration for the first time that bulk and miniemulsion polymers can be produced in a tubular reactor under controlled nitroxide-mediated polymerization conditions, and copolymers can be produced. A detailed residence time distribution study for the tubular reactor is also shown, and several interesting phenomena are discussed that have implications on the practical operating conditions of the tubular reactor. This particular study makes it clear that one should experimentally verify the residence time distribution within a continuous system with the reactants of interest, and that model systems may not give an accurate picture of the real system. / Thesis (Ph.D, Chemical Engineering) -- Queen's University, 2010-12-20 12:00:37.974

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<p>The focus of this study is to synthesize a novel hinged polymer actuator. The linking molecule (hinge) is phenazine with interconnected dipyrrole units.</p>

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