The primary goals of this research were to develop an improved design for measuring tympanic temperature in groups of beef cattle, determine the ideal operating parameters of the new device, and to verify its effectiveness in a research application. Development of the continuous tympanic temperature logger (CTTL) consisted of two steps: identifying a small temperature logger capable of adequate data storage and constructing a probe to hold the logger and fit in the ear canal of a bovine animal. The minimum sampling interval needed to measure tympanic temperature in beef cattle was calculated using Fourier analysis. In addition, the differences in core body temperature between three measurement locations (left ear, right ear, and vagina) were quantified. The CTTL was also used to record the thermoregulatory responses of feedlot heifers with access to shade provided by three different materials.

Thesis (Ph.D.)--Indiana University, 2010. / Title from screen (viewed on March 3, 2010). Department of Pharmacology and Toxicology, Indiana University-Purdue University Indianapolis (IUPUI). Advisor(s): Joseph A. DiMicco, Sherry F. Queener, Daniel E. Rusyniak, Michael R. Vasko. Includes vitae. Includes bibliographical references (leaves 126-147).

Tyrimo objektas: miego ir paros ciklo įtaka atminčiai, sveikiems asmenims. Darbo tikslas: nustatyti kaip miegas ir paros ciklas veikia sveikų žmonių atmintį. Darbo uždaviniai: 1. Išsiaiškinti, kaip vidinės kūno temperatūros pokyčiai veikia nuotaiką ir mieguistumą. 2. Ištirti, kaip paros ciklo metu kinta atminties aktyvumas. 3. Palyginti atminties aktyvumą po išsimiegotos nakties su atminties aktyvumu po bemiegės nakties. Tyrimo hipotezės: vidinės kūno temperatūros svyravimai atspindi atminties aktyvumą. Tyrimo metodai: Mokslinės literatūros analizė. Empirinis tyrimas naudojat kompiuterinę testų programą ANAM4. Statistinė duomenų analizė. Išvados: 1. Vidinė kūno temperatūra veikia, mieguistumą ir nuotaiką. Kai nakties metu vidinė kūno temperatūra krenta, padidėja mieguistumas ir pablogėja nuotaika, o kai dienos metu vidinė kūno temperatūra kyla, mažėja mieguistumas ir pagerėja nuotaika. 2. Vidinės kūno temperatūros svyravimai atspindi darbinės atminties aktyvumo svyravimus, kai reikia atsiminti išmoktus objektus. O atliekant matematinius skaičiavimus ir erdvinio suvokimo užduotis, atmintis aktyvumo svyravimai nesutapo su vidinės kūno temperatūros svyravimais. 3. Atmintis aktyvumas yra geresnis dieną po išsimiegotos nakties, lyginant su diena po bemiegės nakties. / The object of research: sleep and circadian rhythm effects on healthy people memory. The aim of research: to find out how sleep and circadian rhythm effects memory in healthy The goals of research: 1. Find out, how core body temperature effects mood and sleepiness. 2. Determine, how memory activity changes in circadian rhythm. 3. Compare memory activity after sleep night with memory activity after sleepless. Hypothesis: core body temperature reflects fluctuations in memory activity. Research methods: Scientific literature analysis. Empiric research using computer cognitive tests program ANAM4. Statistical data analysis. Conclusions: 1. Core body temperature affects sleepiness and mood. When at night time core body temperature decreases, sleepiness increase and mood impairs, and when at day time core body temperature increases, sleepiness decreases and mood improves. 2. Core body temperature fluctuations reflects memory activity, when need to recognize learned objects. In mathematical computation and spatial processing, memory activity does not match with core body temperature fluctuations. 3. Memory activity is higher during day after sleep night, comparing with memory activity after sleepless night.

Transporting cattle from southern Alberta into the United States (US) plays a substantial economic role in the western Canadian beef industry. Thermal environments within cattle transport trailers are dependent on ambient conditions, and if inadequately managed, can be a welfare concern. To effectively manage cattle transport, the environmental conditions throughout the livestock trailer must be understood. The objective of the present study was to investigate the trailer micro-climate and welfare during 5-paired commercial long-haul transports of slaughter cattle from Alberta, Canada to Washington State, US during summer months. In addition, the effect of compartment location and trailer porosity (8.7% vs 9.6%) on trailer micro-climate, shrink and core body temperature were also investigated during the warmest in-transit hour and stationary events. The compartment location had an effect on micro-climate variables where the upper compartment had greater (P < 0.05) temperature than the bottom deck compartments and relative humidity variables had the opposite effect for both the warmest in-transit hour and stationary events. There was also an effect of trailer porosity on micro-climate variables where it was generally warmer in the trailer with the higher porosity in the stationary event. Differences between trailers included 2 additional roof hatches on the trailer with lower side-wall porosity and lower internal temperatures, which could suggest the location of the trailer porosity, could be important for heat and moisture exchange during transit. The nose of the trailer with higher porosity had generally warmer internal conditions (larger T(trailer)°C and THI(trailer)) than the trailer with lower porosity. This study also found that the temperatures inside the trailer can be 10.5°C greater than ambient temperatures during stationary events and 9°C greater than ambient levels during the warmest in-transit hour. The average amount of per-animal weight loss was 4.3 0.3 % and was affected by trailer porosity and compartment, which followed the trends in thermal environment variables. The transit status (stationary or in-transit) and trailer porosity affected the vaginal core body temperature of the heifers in transit. The core body temperature was greater during stationary events for animals transported in the trailer with lower porosity. It is suggested that the lower side-wall porosity and/or the shape of perforation pattern could impair the movement of fresh air to the respiratory tract of heifers, thus impacting the main mechanism for dissipating heat. The difference in temperature from the trailer ceiling to the animal level was 3.38°C in the trailer with lower porosity (cooler at the ceiling) and 2.23°C in the trailer with the higher porosity. This relationship also had a compartment location effect that followed the micro-climate compartmental differences. This could suggest that excess heat in the trailer with the lower porosity, that also had lower overall temperatures, exited through roof hatches, while in the trailer with the higher porosity, the heat escaped through the side-wall perforations. This theory also supports the idea that the location of where the porosity is located on the trailer may be important to alleviating heat stress in summer months during transport. The results of this study also indicated that there was no difference in the location of the data logger plane (driver, middle passenger) and within the compartments (front, middle, back), suggesting that compartment location effect is substantial when considering micro-climate but temperatures within a compartment are mostly homogenous. The trip that had average ambient temperatures of 25.9 ± 6.06°C for the entire journey, had a temperature Humidity Index that was considered in the danger or emergency category according to the Livestock Weather Heat Index during 95% of the warmest in-transit hour. This suggests that during ambient temperatures of 25.9°C, both trailers used in this study did not have sufficient heat exchange to mitigate the risk of heat stress for cattle.

Ten canines were utilized in a repeated design to examine the effects of stress associated with work on activity expenditure, core body temperature, and salivary cortisol. However, only eight canines had valid data results due to the ninth canine having a metabolic syndrome that was not diagnosed. The tenth canine was removed from the study prior to initiation of the search scenario due to aggression. The objective was to observe the impact of stressors associated with work on activity expenditure, core body temperature, and salivary cortisol in human remains detection canines. Nine canines were randomly assigned to 1 of 3 simulated search sites which each had two repetitions 1A) RU (rubble area with two concealed partial cadavers); 1B) RU (open crop field with one concealed partial cadaver); 2A) WA (wooded area with two concealed partial cadavers elevated off the ground); 2B) WA (wooded area with two concealed partial cadavers); 3A) MC (mass casualty area with eight exposed full body cadavers); 3B) MC (mass casualty area with five buried full body cadavers). Canines searched for an average of 90 minutes at each site which was then followed by a 10 minute rotation period where canines randomly rotated to one of the other two sites. Upon completion of the third site, canines repeated the first site location. There was a 69 minute rest period between the second and third rotation. Canines were rotated through search sites such that one canine was actively working (AW), the next canine was waiting to work (WW) and was exposed to visual and auditory stimulation in preparation for work and the third canine in the rotation was not working (NW) and crated in the handler’s vehicle with no visual or auditory stimulation associated with work 100 feet from WW area. Total activity expenditure was not influenced by site (P = 0.89) which may indicate detection canines were efficient in expending energy during this study. This correlated with the walk activity expenditure by site as it did not differ based on site (P = 0.16). The canines expended a similar amount of walk energy at each site and this may demonstrate that canines spent more time searching and in scent of the cadaver(s). Conversely, run activity expenditure was influenced by site (P = 0.03), specifically RU site was greater than MC site. When further comparison of the RU repetitions was analyzed, the second repetition was greater than the first in run activity expenditure (P < 0.0001). Canines at the RU site during the second repetition had an easier ability to run compared to the first repetition because of the nature of the site. Total activity expenditure was influenced by rotation (P = 0.04) but was not influenced by rotation within site (P = 0.17). The first rotation was greater than the fourth rotation which may indicate a psychological stressor such as anticipation of the work and possible acclimation of the site. The fourth rotation was at the same location site as the first rotation and canines did not have the novelty of the site as a psychological stressor during the fourth rotation. Canines that did not have exposure to full body cadavers prior to this study did not have greater total activity expenditure (P = 0.46) at the MC site compared to canines that did have exposure to full body cadavers prior to the study. There was an effect of phase on core body temperature (P = 0.0003). However, 95.3% of canines had their core body temperature peaked in the NW phase directly after the AW phase. Which demonstrates that core body temperature continued to increase post exercise. There was also a plateau in the core body temperature for 18 minutes post exercise which could indicate peripheral vasodilation. Attenuation of core body temperature occurred 37 minutes after cessation of the AW phase. There was an effect of site (P < 0.0001), rotation (P < 0.0001), and rotation within site (P < 0.0001) on core body temperature. This may indicate that there was a boundary layer stimulus that acted as a psychological stressor. In addition, the second and third rotation were greater than the first and fourth rotation which is most likely due to the increase in humidity during the second rotation. Core body temperature was impacted by total activity expenditure (P < 0.0001) which is to be expected with increased exercise, core body temperature increases. Salivary cortisol was influenced by site (P = 0.003), specifically the WA site was greater than the MC and the RU site. The WA site may have had a stronger psychological stressor with the tree lines compared to the MC and the RU sites. Rotation within site did impact salivary cortisol (P < 0.0001). However, rotation alone did not influence salivary cortisol (P = 0.42). Core body temperature did not impact salivary cortisol (P = 0.18). Total activity expenditure did not affect salivary cortisol (P = 0.73). Salivary cortisol and age of canine did display a pattern (P = 0.003). Salivary cortisol and exposure to prior full body cadaver did not displayed a pattern (P = 0.78). Based on these results, some physical stressors such as site, rotation, and exercise impacted activity expenditure, core body temperature, and salivary cortisol. This thesis provides limited data dissemination on three physiological parameters that may be impacted on independent variables that are associated with search scenario work. More research is needed to confirm if these specific stressors observed impact activity expenditure, core body temperature, and salivary cortisol in human remains detection canines.

Master of Science / Animal Sciences and Industry / Micheal J. Brouk / Study 1 was performed to assess the effect of an evaporative cooling system (ECS) on core body temperature (CBT) and lying times in lactating dairy cows. Respiration rates and rear udder temperatures (T[subscript u]) were also measured. Trial 1 contained 3 environmental treatments while trial 2 contained 2 environmental treatments. Treatments were: OFF (Cyclone fans and fog shut off), FAN (Cyclone fans only, no fog), and FANFOG (Cyclone fans and fog on) and cows exposed to these 3 environments were housed in a bedded pack barn (PACK) equipped with an ECS or a tie-stall barn (TIE). TIE and PACK cows moved between barns every 8 h for milking and both groups moved opposite of each other. Ambient, barn temperature and relative humidity (RH) measurements in addition to vaginal temperatures and lying times were recorded by sensors which took measurements at 1 min intervals. Respiration rates (BPM), for PACK cows during FANFOG were reduced (P < 0.05) when compared to TIE (53 ± 2.0 vs 64 ± 2.0, respectively) in trial 1. Similar results were found in trial 2. Rear udder temperature was measured and found to be decreased (P < 0.05) in PACK cows while housed under FANFOG vs TIE in trial 1. These results could not be repeated in trial 2 because of greater ambient temperatures. Core body temperature (CBT) was reduced during each trial as shown by less time spent above 39.0°C during FANFOG. During trial 2, PACK spent 5.7 and 8.5 h/d less over a CBT of 39.0°C compared to TIE cows. Total daily lying time was tracked and found to increase for cows exposed to the ECS during PACK but no difference between PACK and TIE. In study 2, the same ECS was used but its effects on nonlactating dairy cows were studied. There was a treatment by h interaction for vaginal temperature showing the greatest effects during the afternoon h where FANFOG had numerically decreased CBT vs FAN. FANFOG cows spent reduced time over a CBT of 39.0°C and greater time < 38.0°C. FANFOG cows also had increased lying times of 1.7 h/d compared to FAN.

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