Made available in DSpace on 2014-06-11T19:33:37Z (GMT). No. of bitstreams: 0 Previous issue date: 2011-07-20Bitstream added on 2014-06-13T19:44:30Z : No. of bitstreams: 1 alves_au_dr_jabo.pdf: 1100641 bytes, checksum: 13f3398f1e24941f73ea3e93de845ff8 (MD5) / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) / Com objetivo de avaliar o desempenho agroeconômico da berinjeleira e do tomateiro, em cultivo consorciado, em relação a seus cultivos solteiros, em função da época de transplante da berinjeleira em relação ao do tomateiro e da época de cultivo. Foram realizados dois experimentos em campo, em Jaboticabal, São Paulo, Brasil, (12 de fevereiro a 5 de setembro 2009 e 8 de agosto de 2009 a 20 de fevereiro 2010. Em cada experimento foram avaliados 21 tratamentos, sob blocos ao acaso, em esquema fatorial 2 x 10 + 1, com quatro repetições. Os fatores avaliados foram sistemas de cultivo (consórcio e solteiro) e épocas de transplante da berinjeleira após o transplante do tomateiro (-30, -25, -20, -15, -10, -5, 0, +5, +10 e +15 dias) e o cultivo solteiro do tomateiro. Na primeira época de cultivo, transplantes mais tardios da berinjeleira causaram decréscimos no número de frutos, produção comercial e produtividade comercial em razão da maior interferência do tomateiro na berinjeleira e das menores temperaturas, enquanto na segunda época de cultivo, os decréscimos foram motivados somente pela interferência do tomateiro. Os componentes da produção do tomateiro foram maiores à medida que mais tardio foi o transplante da berinjeleira. Maiores no número de frutos, produção comercial e produtividade comercial do tomateiro e da berinjeleira foram obtidos na segunda época de cultivo. A mão-deobra é a componente com maior participação no custo operacional total das culturas consorciadas ou do cultivo solteiro de berinjeleira e tomateiro. Nos consórcios, as maiores receitas, taxas e margens de retorno são obtidas nos consórcios na segunda época de cultivo. Do ponto de vista econômico, o tomateiro foi a cultura dominante e a berinjeleira foi a dominada / Two experiments were carried out under field conditions in Jaboticabal, SP, Brazil (21o15’22’’S, 48o18’58’’W and altitude 575m), in order to evaluate the productive performance of eggplant and tomato, in intercropping system, in function of transplanting times of eggplant in relation to tomato transplanting and of growing times (2-12 to 9-5-2009 and 8-8-2009 to 2-20-2010). Each experiment was carried out in a randomized complete block design with 21 treatments (2 x 10 + 1) and four replications. The treatments consisted of the combination of two cropping systems (intercropping and sole crop) and ten eggplant transplanting times (-30, -25, -20, -15, - 10, -5, 0, +5, +10 and +15 days after tomato transplanting) and tomato sole crop. In the first growing time, transplants later of eggplant caused decreases in the number of fruits per plant, commercial production and business productivity yield due the interference of tomato in the eggplant and low temperatures, whereas in the second growing time, the decreases in yield component of eggplant were motivated only by competition with the tomato. For tomato, there was a significant interaction between growing times and eggplant transplanting times in number of marketable fruits per plant marketable yield per plant and commercial yield. The yield components of tomato were higher as was the later transplant of eggplant. Higher number of marketable fruits per plant marketable yield per plant and commercial yield.of tomato and eggplant were obtained in the second growing time. The workmanship is the component with greater participation in total operating cost of intercropping or monocropping of eggplant and tomato. In the consortium, the higher revenues, margins and rates of return are obtained in the consortia in the second growing season. The point view economic Tomato was the dominant culture and eggplant was dominated

Made available in DSpace on 2014-06-11T19:33:38Z (GMT). No. of bitstreams: 0 Previous issue date: 2010-05-18Bitstream added on 2014-06-13T18:45:40Z : No. of bitstreams: 1 charlo_hco_dr_jabo.pdf: 339831 bytes, checksum: 53edd19c24ce9c5b7dfd8a470866bb02 (MD5) / Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) / Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) / Com o objetivo de avaliar o desempenho de híbridos experimentais de melão rendilhado em dois sistemas de cultivo e o desempenho de híbridos de melão rendilhado em diferentes substratos foram desenvolvidos dois experimentos no Setor de Olericultura e Plantas Aromático-Medicinais da UNESP, Jaboticabal, SP. O primeiro experimento foi desenvolvido de 20 de setembro de 2008 a 05 de janeiro de 2009. Foram avaliados seis híbridos experimentais, Jab 07#16, Jab 07#17, Jab 07#23, Jab 07#24, Jab 07#26, Jab 07#28, e três híbridos comerciais de melão rendilhado, Bônus nº 2, Louis e Fantasy, em dois sistemas de cultivo (em solo e em fibra da casca de coco), adotando-se o manejo recomendado para a cultura, em cada um dos sistemas. O delineamento experimental utilizado foi em blocos casualizados, com nove tratamentos e quatro repetições, para cada sistema de cultivo, realizando-se análise conjunta dos dados. Concluiu-se que, para o cultivo em fibra da casca de coco todos os híbridos são recomendados, exceto o Jab 07#17. No cultivo em solo recomendam-se os híbridos Bônus n° 2, Fantasy, Jab 07#26, Jab 07#28 e Jab 07#16. No segundo experimento foram avaliados cinco substratos (S1 = fibra da casca de coco; S2 = areia; S3 = ½ areia e ½ bagaço de cana-de-açúcar; S4 = ½ areia e ½ casca de amendoim; e S5 = areia, bagaço de cana-de-açúcar e casca de amendoim), e quatro híbridos de melão rendilhado (Bônus n° 2, Louis, Fantasy e Jab 2007#16). Utilizaram-se vasos plásticos de 13 dm3 que foram dispostos no espaçamento de 1 m entre fileiras e 0,5 m entre vasos. O delineamento experimental foi em blocos casualizados, em esquema fatorial 5 x 4, com quatro repetições. Concluiu-se que a melhor opção de substrato é a combinação de 50% de areia e 50% de casca de amendoim, e como segunda opção a combinação de areia, bagaço de cana-de-açúcar e casca de amendoim em partes iguais... / Aiming evaluate the net melon experimental hybrids in two crop systems as well the net melon hybrids performance in different substrates were carried out two experiments in the Setor de Olericultura e Plantas Aromático-Medicinais da Faculdade de Ciências Agrárias e Veterinárias (UNESP-Jaboticabal Vegetables and Aromatic- Medicinal Plants Sector). The first experiment was carried out from Sept-20-2008 to Jan- 05-2009, where were evaluated six experimental hybrids (Jab 07#16, Jab 07#17, Jab 07#23, Jab 07#24, Jab 07#26, and Jab 07#28) and three commercial hybrids (Bônus nº 2, Louis, and Fantasy), in two crop systems (soil and coconut fiber cropping), adopting the recommended management to the melon crop in each of the two crop systems. The experimental design used was the randomized blocks with nine treatments and four replications to each cultivation system. The both experiment data analysis was done joint. Productive and qualitative fruit characteristics were evaluated and, based on the results, the crop system under coconut fiber all hybrids are recommended, except the hybrid Jab 07#17, while only the hybrids Bônus n° 2, Fantasy, Jab 07#26, Jab 07#28, and Jab 07#16 should be cultivated in the conventional (soil) system. In the second experiment were evaluated five substrates (S1= coconut fiber; S2= sand; S3= ½sand + ½sugar cane bagasse; S4= ½sand + ½peanut fiber; and S5= sand + sugar cane bagasse + peanut fiber in four net melon hybrids - Bônus n° 2, Louis, Fantasy, and Jab 2007#16). Plastic pots of 13dm3 each were filled up with each substrate and spaced 1m between lines and 0.5m between plots. The experimental design used was the randomized blocks, in a 5x4 factorial scheme and four replications. The fruit productive and qualitative characteristics were evaluated and based on the results, the best substrate option to cultivate net melon is the 50%sand + 50% peanut fiber... (Complete abstract click electronic access below)

Submitted by Camila Seno Nascimento (camilaseno@gmail.com) on 2018-04-19T00:57:07Z No. of bitstreams: 1 dissertacao_Camila_Seno_Nascimento.pdf: 983641 bytes, checksum: 512ebabb21bfd537b166db61ac06cce7 (MD5) / Approved for entry into archive by Alexandra Maria Donadon Lusser Segali null (alexmar@fcav.unesp.br) on 2018-04-19T11:20:37Z (GMT) No. of bitstreams: 1 nascimento_cs_me_jabo.pdf: 983641 bytes, checksum: 512ebabb21bfd537b166db61ac06cce7 (MD5) / Made available in DSpace on 2018-04-19T11:20:37Z (GMT). No. of bitstreams: 1 nascimento_cs_me_jabo.pdf: 983641 bytes, checksum: 512ebabb21bfd537b166db61ac06cce7 (MD5) Previous issue date: 2018-02-21 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / O correto estabelecimento e balanceamento da relação N:K para cada estádio fenológico do meloeiro é fundamental para promover a maximização do crescimento e desenvolvimento da planta e evitar desordens nutricionais. Desta forma, com o objetivo de avaliar a influência de concentrações de nitrogênio (N) e potássio (K) em cada fase fenológica (vegetativa e reprodutiva) do meloeiro e obter a melhor relação N:K para cada uma delas, um experimento foi conduzido em casa de vegetação, em sistema hidropônico. Na primeira fase, foram avaliadas quatro concentrações de N (8, 11, 14 e 17 mmol L-1) e duas concentrações de K (4 e 5 mmol L-1), em delineamento experimental de blocos ao acaso, esquema fatorial 4x2, com cinco repetições. Na segunda fase, em esquema fatorial 2x2, foram avaliadas as combinações das duas concentrações de N que geraram as melhores características na fase anterior (maior área foliar, altura de planta e número de folhas) e duas concentrações de K (4,5 e 9,0 mmol L-1). Com base nas características de crescimento, a melhor relação N:K para a fase vegetativa foi a de 17:5 mmol L-1. O aumento das concentrações de N na fase vegetativa promoveu o aumento dos teores foliares de N, P e S e a redução de K, Ca e Mg. As concentrações de N e K não influenciaram a área foliar, produtividade e qualidade dos frutos do meloeiro, e assim considerou-se 14:4,5 mmol L-1 como a melhor relação N:K para a fase reprodutiva. / The correct establishment and balancing of the N:K ratio for each phenological growth stage of melon is essential to maximize the plant growth and development and avoid nutritional disorders. Thus, in order to evaluate the influence of nitrogen (N) and potassium (K) concentrations in each phenological growth stage (vegetative and reproductive) of melon plants and obtain the best N:K ratio for each of them, an experiment was carried out in a greenhouse, in a hydroponic system. In the first stage, four N concentrations (8, 11, 14, and 17 mmol L-1) and two K concentrations (4 and 5 mmol L-1) were evaluated. The experimental design adopted was a randomized block in a 4x2 factorial scheme with five replicates. In the second stage, in a 2x2 factorial scheme, the combination of the two N concentrations which generated the best characteristics in the previous stage (higher leaf area, plant height and number of leaves) and two K concentrations (4.5 and 9.0 mmol L-1) were evaluated. Based on the growth characteristics, the best N:K ratio for the vegetative stage was 17: 5 mmol L-1. The increase of N concentrations in the vegetative stage promoted the increase of leaf N, P, and S content and the decrease of K, Ca and Mg. The N and K concentrations did not influence the leaf area, yield and fruit quality, so 14:4.5 mmol L-1 was considered as the best N: K ratio for the reproductive stage.

Resumo: Com objetivo de avaliar o desempenho agroeconômico da berinjeleira e do tomateiro, em cultivo consorciado, em relação a seus cultivos solteiros, em função da época de transplante da berinjeleira em relação ao do tomateiro e da época de cultivo. Foram realizados dois experimentos em campo, em Jaboticabal, São Paulo, Brasil, (12 de fevereiro a 5 de setembro 2009 e 8 de agosto de 2009 a 20 de fevereiro 2010. Em cada experimento foram avaliados 21 tratamentos, sob blocos ao acaso, em esquema fatorial 2 x 10 + 1, com quatro repetições. Os fatores avaliados foram sistemas de cultivo (consórcio e solteiro) e épocas de transplante da berinjeleira após o transplante do tomateiro (-30, -25, -20, -15, -10, -5, 0, +5, +10 e +15 dias) e o cultivo solteiro do tomateiro. Na primeira época de cultivo, transplantes mais tardios da berinjeleira causaram decréscimos no número de frutos, produção comercial e produtividade comercial em razão da maior interferência do tomateiro na berinjeleira e das menores temperaturas, enquanto na segunda época de cultivo, os decréscimos foram motivados somente pela interferência do tomateiro. Os componentes da produção do tomateiro foram maiores à medida que mais tardio foi o transplante da berinjeleira. Maiores no número de frutos, produção comercial e produtividade comercial do tomateiro e da berinjeleira foram obtidos na segunda época de cultivo. A mão-deobra é a componente com maior participação no custo operacional total das culturas consorciadas ou do cultivo solteiro de berinjeleira e tomateiro. Nos consórcios, as maiores receitas, taxas e margens de retorno são obtidas nos consórcios na segunda época de cultivo. Do ponto de vista econômico, o tomateiro foi a cultura dominante e a berinjeleira foi a dominada / Abstract: Two experiments were carried out under field conditions in Jaboticabal, SP, Brazil (21o15'22''S, 48o18'58''W and altitude 575m), in order to evaluate the productive performance of eggplant and tomato, in intercropping system, in function of transplanting times of eggplant in relation to tomato transplanting and of growing times (2-12 to 9-5-2009 and 8-8-2009 to 2-20-2010). Each experiment was carried out in a randomized complete block design with 21 treatments (2 x 10 + 1) and four replications. The treatments consisted of the combination of two cropping systems (intercropping and sole crop) and ten eggplant transplanting times (-30, -25, -20, -15, - 10, -5, 0, +5, +10 and +15 days after tomato transplanting) and tomato sole crop. In the first growing time, transplants later of eggplant caused decreases in the number of fruits per plant, commercial production and business productivity yield due the interference of tomato in the eggplant and low temperatures, whereas in the second growing time, the decreases in yield component of eggplant were motivated only by competition with the tomato. For tomato, there was a significant interaction between growing times and eggplant transplanting times in number of marketable fruits per plant marketable yield per plant and commercial yield. The yield components of tomato were higher as was the later transplant of eggplant. Higher number of marketable fruits per plant marketable yield per plant and commercial yield.of tomato and eggplant were obtained in the second growing time. The workmanship is the component with greater participation in total operating cost of intercropping or monocropping of eggplant and tomato. In the consortium, the higher revenues, margins and rates of return are obtained in the consortia in the second growing season. The point view economic Tomato was the dominant culture and eggplant was dominated / Orientador: Arthur Bernardes Cecílio Filho / Coorientador: Bráulio Luciano Alves Rezende / Coorientador: Francisco Bezerra Neto / Banca: Durvalina Maria Mathias dos Santos / Banca: Pablo Forlan Vargas / Banca: Jairo Osvaldo Cazetta / Banca: Hamilton César de Oliveira Charlo / Doutor

Os Planctomycetes são bactérias que possuem características peculiares, ainda pouco conhecidas. São bactérias de difícil cultivo, sendo descritas em diversos ambientes, mas frequentemente isoladas de ambientes marinhos, principalmente em associações com algas. Aqui apresentamos um amplo estudo deste grupo em solos de manguezais, e reportamos de maneira inovadora o isolamento dessas bactérias. A comparação das comunidades de Planctomycetes em manguezais com diferentes históricos de contaminação permitiu fazer inferências sobre a resposta da comunidade ao ambiente. A análise de sequências pertencentes a este filo, obtidas a partir de amostras dos manguezais - tanto sequências do gene 16S DNAr ou sequências metagenômicas - permitiu inferir sobre a diversidade e as funções destes grupos nos solos dos manguezais estudados. Destacam-se dentre estas o aumento da biodiversidade deste grupo em áreas contaminadas, e as evidências de sua participação na degradação de xenobióticos (demonstrada por predição metagenômica baseada em biblioteca de 16S rDNA, e análise de sequencias metagenômicas). Com estes resultados, foram encontrados em dados metagenômicos, a ocorrência de genes envolvidos na biodegradação de compostos intermediários centrais das vias de degradação. Adicionalmente, mesmo com a dificuldade no cultivo de membros desse grupo, foram obtidos 43 isolados afiliados filogeneticamente principalmente às espécies Blastopirellula marina, Rhodopirellula baltica e Planctomycetes sp. e Pirellula. Foram selecionados dois isolados (Rhodopirellula sp. MGV e Rhodopirellula baltica BR-MGV) para estudos genômicos e metabólicos (via análise de consumo de diferentes fontes de carbono-Biolog). Além disso, foi realizado teste de degradação de hidrocarbonetos com os 43 isolados aferindo as respostas ao contato com contaminantes como hexadecano, naftaleno, fenantreno e fenol. Cinco isolados mostraram a capacidade em degradar três diferentes hidrocarbonetos exceto fenantreno. Estes isolados (Blastopirellula cremea Hex-1 MGV, Blastopirellula marina Nap-Phe MGV, Blastopirellula cremea Hex-2 MGV, Blastopirellula cremea Hex PRIS-MGV e Blastopirellula marina Nap PRIS-MGV) foram enviados a uma análise genômica e suas anotações indicaram a presença de genes envolvidos em vias de degradação de hidrocarbonetos corroborando com o teste realizado em laboratório de degradação dos hidrocarbonetos. Além disso, foi observada através das anotações dos genomas desses microrganismos a ocorrência de síntese de metabólitos secundários, sendo os principais terpenos, bacteriocinas e resorcinol. / Planctomycetes are bacteria with peculiar characteristics and still little known. They are bacteria of difficult cultivation, being described in diverse environments, but often isolated from marine environments, mainly in associations with algae. Here we present an extensive study of this group in mangrove soils and report in an innovative way the isolation of these bacteria. The comparison of the communities of Planctomycetes in mangroves with different contamination histories allowed to make inferences about the response of the community to the environment. The analysis of sequences from this phylum, obtained from samples of the mangroves - both sequences of the 16S DNAr gene or metagenomic sequences - allowed to infer about the diversity and the functions of these groups in the mangrove soils studied. The highlights are the increase of this group\'s biodiversity in contaminated areas, and evidence of its participation in the degradation of xenobiotics (demonstrated by 16S rDNA library-based metagenomic prediction and metagenomic sequence analysis). With these results, the occurrence of genes involved in the biodegradation of central intermediates of the degradation pathways was found in metagenomic data. In addition, even with the difficulty in the cultivation of members of this group, 43 isolates belonging to this phylum were obtained phylogenetically, mainly the species Blastopirellula marina, Rhodopirellula baltica and Planctomycetes sp. and Pirellula. Two isolates (Rhodopirellula sp. MGV and Rhodopirellula baltica BR-MGV) were selected for genomic and metabolic studies (via consumption analysis of different sources of carbon-Biolog). Furthermore, a hydrocarbon degradation test was performed with this library of 43 isolates, assessing the responses to contact with contaminants such as hexadecane, naphthalene, phenanthrene and phenol. Five isolates showed the ability to degrade three different hydrocarbons except phenanthrene. These isolates (Blastopirellula cremea Hex-1 MGV, Blastopirellula marina Nap-Phe MGV, Blastopirellula cremea Hex-2 MGV, Blastopirellula cremea Hex PRIS-MGV and Blastopirellula marina Nap PRISMGV) were sent to a genomic analysis and their notes indicated the presence of genes involved in hydrocarbon degradation pathways, corroborating with the laboratory test for hydrocarbon degradation. In addition, the synthesis of secondary metabolites was evaluated through the annotations of the genomes of these microorganisms, being the main terpenes, bacteriocins and resorcinol.

The demand for organic produce is based on a general belief that organically grown produce is more nutritious than conventionally cultivated produce. To date, there have been several studies both supporting and contradicting these assumptions and at this point there is no clear consensus. However, there has been one accepted and appreciated aspect of the organic cultivation, which is, that it renders the soil more suitable for long-term cultivation and improves the ecological aspect of producing produce. For this reason, in the long term organic farming may be both economically and ecologically more desirable. The focus of this project as a whole is to study conventional and organic methods for peach cultivation to better understand them and to determine the most economically and ecologically desirable method of peach cultivation in Utah. This specific experiment involved evaluating physicochemical properties of peaches grown under 6 different organic treatments (peaches grown in a certified organic orchard using six different organic treatments) and cultivated using 5 different conventional treatments (peaches grown in a conventional orchard). Peaches were harvested on four different harvest dates to determine the effect of time of cultivation on peach fruit quality. Several different quality attributes of peaches were evaluated. Peaches cultivated under six different organic treatments were statistically compared to determine the difference in their quality attributes. Similarly, peaches cultivated under five different conventional treatments were compared statistically to determine the difference in their quality attributes. Effect of organic treatment on peach quality was not statistically compared with the effect of conventional treatment on peach quality as both treatments were used in separate orchards. No significant differences were observed in quality attributes of either variety of peaches subjected to 6 different organic treatments, nor were any differences observed amongst peaches subjected to 5 different conventional treatments. Moreover, it was observed that peaches harvested on early dates (typically 1 and 2) had more desirable quality attributes. It is interesting that the treatments affected peach growth and development, and future work will involve a correlation with sensory, and volatile analysis.

The disparity between the ongoing use, the overall positive attitudes towards criminal profiling and the lack of empirical evidence for its validity is also referred to as criminal profiling illusion. Associated risks for society range from misled police investigations, hindered apprehensions of the actual offender(s), and wrongful convictions of innocent citizens to mistrust in the police and their methods. Research on potential explanations to the Criminal Profiling Illusion is still in its infancy but assumes that people receive and adopt incorrect messages favouring the accuracy and utility of criminal profiling. One suggested mechanism through which individuals may acquire such incorrect messages is the consumption of fictional crime-related media which typically present criminal profiling as highly accurate, operationally useful and leading to the apprehension of the offender(s). By having some relation to reality but presenting a distorted picture of criminal profiling, fictional crime-related media may blur the line between fiction and reality thereby increasing the risk for the audience to mistake fiction for fact. Adopting a cultivation approach adequate to examine media effects on one’s perception, the present study is the first to investigate whether the perception of criminal profiling may be influenced by the consumption of fictional crime-related media based on a correlation study. Although the results provide support for the assumption that misperceptions of criminal profiling are widely spread in the general population and associated with the consumption of fictional crime-related media, the found cultivation effects are small and must be interpreted cautiously. Considering that even small effects may have the potential to influence real-life decision-making, they may still be relevant and affect the society at large.

Thesis (M.Sc.(Crop Science) North-West University, Mafikeng Campus, 2011

Vampirovibrio chlorellavorus (Gromov et Mamkaeva, 1980) is a member of the phylum cyanobacteria that has been described as an obligate pathogen of several of the green microalga, Chlorella. It utilizes as yet unknown functions to access the contents of individual Chlorella sp. host cells, which results in cell death. Its presence in a cultivated Chlorella sorokiniana culture was first discovered using polymerase chain reaction (PCR) to amplify the 16S ribosomal DNA gene, followed by DNA sequencing. Its continued routine detection throughout much of the cultivation season suggested it was an endemic member of the phycosphere community in this open cultivation system, located in Tucson, Arizona. Ultimately, its presence resulted in rapid death of C. sorokiniana in open pond systems and reduced biomass harvest. PCR analysis of total DNA isolated from sand and soil layers removed from a nearby riverbed indicated that V. chlorellavorus resides naturally in the riverbed. The ability to manage this bacterial pathogen in cultivated Chlorella host species is hindered by the limited information available in the literature regarding the biological and genomic characteristics of V. chlorellavorus. The objective of this study was to identify environmental factors that trigger the apparent increased growth rate of V. chlorellavorus and rapid algal death during the cultivation cycle. In laboratory experiments, V. chlorellavorus was shown to cause death of C. sorokiniana when the temperature exceeded 28°C, whereas, algal death was not observed when the temperature was 24°C or lower, among the temperatures tested. Also, the bacterium was more pathogenic to C. sorokiniana, grown in open cultivation systems during the summer months, compared to the cooler season months. Futhermore, when C. sorokiniana and V. chlorellavorus were co-cultivated in the presence of sodium chloride ranging from 0-10 g per liter, the growth of the bacterium was not impeded to any extent that might suggest C. sorokiniana was rendered less susceptibility to pathogen attack. Future work involves examining more triggers and ways to inhibit V. chlorellavorus growth.

<p>The purpose of the study was to set up a method for cultivation of C. pneumoniae from infected mouse tissue in Hep2 cells. We also evaluated a new reagent, Chlamatis, which is considered to increase the detection sensitivity of the bacterium with both PCR and cultivation. All 10 serum samples treated with Chlamatis were negative for C. pneumoniae in PCR. The cultivation of tissue was found to work without problems. The yield of the bacteria was highest at the speed of 30 Hz using homogenization with TissueLyser. Mice infected with C. pneumoniae were killed at days 4, 8, 20 and 40. The highest yields of C. pneumoniae were found at days 4 and 8 using PCR with all infected mice. The results obtained with PCR and cultivation confirmed each other to a large extent. For heart tissue, PCR positive mice were found only at days 4 and 8. The number of PCR positive lung samples confirmed to a large extent the number found by cultivation, except for mice killed after 4 days and 40 days where the results differed slightly. This indicated that the optimization of the cultivation method was successful</p>