Magíster en Ciencias de la Ingeniería, Mención Recursos y Medio Ambiente Hídrico / Determinar de forma confiable y anticipada la cantidad de agua que puede generar una cuenca como respuesta a las forzantes meteorológicas a las que es sometida, es fundamental para la planificación y optimización del uso de este recurso. Un requisito fundamental para determinar esta relación, es contar con caudales correspondientes al estado natural de la cuenca (Cuencas inalteradas) para realizar la calibración, sin embargo, muchas veces, no se cuenta con mediciones en las zonas que desean estudiarse (cuencas no controladas) o las mediciones registradas, no corresponden al caudal natural de la cuenca, debido a extracciones, tomas no contabilizadas, desvíos, etc. El objetivo de este estudio es proponer y analizar una metodología que permita obtener simulaciones con coeficientes de eficiencia aceptables, de los valores registrados en sistemas que tienen un registro de caudal acotado (alterado) registrando sólo parcialmente los caudales generados por la cuenca. Para alcanzar el objetivo, como primer paso, se modela la zona estudiada, aplicando los programas HEC-HMS y MIKE-NAM en modo continuo, para evaluar su capacidad y limitantes para representar el fenómeno, siguiendo las bases teóricas que se sustentan las buenas prácticas del uso de estos modelos. Posteriormente, al verificar la incapacidad de los modelos utilizados de representar de forma adecuada los hidrogramas registrados, se utiliza una herramienta basada en la teoría de Modelos mecanísticos basados en datos (DBM) denominada Conceptual developer Model (CMD), para que la misma en base a los datos obtenidos utilizando los modelos hidrológicos y los datos registrados, simule la operación de la obra de toma que es la que gobierna la cantidad de agua que se capta de la cuenca y es posible de ser registrada. Como resultado de este trabajo se obtiene que una vez aplicado el CMD, pueden generarse hidrogramas comparables a los observados, con coeficientes de eficiencia de Nash Sutcliffe de hasta 0.89. Los resultados obtenidos, reflejan que a través de este procedimiento se puede obtener un pronóstico confiable de los caudales captados, lo cual contribuye en el caso estudiado a la implementación de un sistema de pronóstico a corto plazo (escala semanal como máximo) de caudales aportantes al sistema para la generación hidroeléctrica.

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Researches on bone defects are focused on the use of composites due to the composite and well-organized hierarchical structure of the bone. In this study, it is aimed to develop Polycaprolactone based implants with different organic &amp / #8211 / DBM, HYA- and/or inorganic &amp / #8211 / bioglass, calcium sulfate- compositions for augmenting bone healing. Bioactivity of the discs was evaluated by scanning electron microscopy and EDS analysis after incubation in SBF for 1, 7 and 14 days. All bioglass containing groups showed apatite molecules at different incubation times. Degradation studies demonstrated that only PCL/BG/HYA discs had fast degradation upon incubations in PBS (4 and 6 weeks). Initial mechanical properties of composites were found to be directly related to the composition. However, decreases in disc mechanical properties were also obtained in the same order with the amount of water uptake at composite groups. According to biocompatibility studies investigated with cytotoxicity tests on Saos-2 cells, all groups, except the HYA involving one were found as biocompatible. After in vivo application of discs to critical size defects on rabbit humeri (for 7 weeks), their efficacy on healing was studied with computerized tomography, SEM and biomechanical tests. The results revealed that bone-implant interface formation has started for all groups with high bone densities at the interface of implant groups compared to empty defect sites of negative controls. Also the healing was suggested to be gradual from bone to implant site as microhardness values increased at regions closer to bone. However, regeneration was found to not reach to healthy bone levels.

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Cbf11 and Cbf12 proteins, the members of the CSL transcription factors family, are involved in a wide range of cellular processes in the fission yeast Schizosaccharomyces pombe - among other things they regulate cell adhesion and they have also been implicated in maintenance of genome integrity. At the level of the whole genome we previously identified target loci bound by CSL proteins in vivo. Many of them do not contain any consensus CSL-binding element. There are probably different DNA binding modes of the Cbf11/12 proteins and it has not been known what specific biological function is associated with the particular way of DNA binding. For the purpose of studying CSL DNA binding modes we have worked in this project on the implementation of the DNA binding mutation (DBM), which prevents direct DNA binding of CSL proteins to canonical motif in vitro, into the chromosomal locus of the cbf11 and cbf12 genes. Using the "ura4 selection system" we have successfully constructed the scar-less Cbf12-TAP and Cbf12DBM-TAP knock-ins, i.e. the strains without/with DBM in the open reading frame of Cbf12 where Cbf12 is C- terminally TAP-tagged and contains the intact 3'UTR. In our laboratory we have established the CRISPR/Cas9 system by which we have been able to prepare the Cbf11- TAP strain. We have failed to...

Yes / This study looks at the relationship between big data analytics capability and market performance and how this relationship can be facilitated by adopting disruptive business models in competitive environments.

Orientador: Sergio Antonio de Bortoli / Resumo: O cultivo intensivo de Brassicaceae favorece o aparecimento de algumas pragas como Plutella xylostella (Linnaeus, 1758) (Lepidoptera: Plutellidae), a traça-das-crucíferas, cujos danos podem comprometer seriamente a produção. Esse inseto-praga pode ser controlado tanto com inseticidas sintéticos, como com biológicos, sendo, no entanto, usualmente utilizados os sintéticos em seu combate. A traça-das-crucíferas foi a primeira espécie de inseto a ser constatada resistência a inseticidas biológicos, com o uso indiscriminado de inseticidas sintéticos para o seu controle contribuindo também para a seleção de indivíduos tolerantes ou resistentes, além de causar a mortalidade de organismos não alvos. Sendo assim, este trabalho teve por objetivo avaliar a atividade inseticida e os efeitos subletais de duas toxinas lepidóptero-ativas de Bacillus thuringiensis, Cry1Ca e Cry2Ac, em três populações de traças-das-crucíferas, sendo uma delas suscetível (PC) e duas consideradas resistentes (Bt e NO-QA) a toxinas de B. thuringienis, em condições de laboratório. Foram realizados bioensaios de suscetibilidade com as três populações e as toxinas Cry1Ca e Cry2Ac, estimando-se a concentração letal (CL50) e os seus efeitos subletais. A toxina Cry2Ac não causou mortalidade nas lagartas das três populações, então os testes subletais foram realizados apenas com a toxina Cry1Ca que provocou mortalidade. Os parâmetros biológicos avaliados foram: período e peso pupal, sobrevivência larval e pupal, longevi... (Resumo completo, clicar acesso eletrônico abaixo) / Abstract: Intensive brassics cultivation favors appearing of some pests as Plutella xylostella (Linnaeus, 1758) (Lepidoptera: Plutellidae), diamondback moth (DBM), whose damage can seriously compromise production. This insect-pest can be controlled either synthetic insecticides as biological insecticides, but synthetics are usually used to combat. Diamondback moth was the first insect species to be verified resistance to biological insecticides, with indiscriminate use of synthetic insecticides for its control, also contributing to selection of tolerant or resistant individuals, as well as causing mortality on non-target organisms. Therefore, the objective of this work was to evaluate insecticide activity and sublethal effects of two lepidopteran-active toxins of Bacillus thuringiensis, Cry1Ca and Cry2Ac, on three DBM populations, one of them susceptible (PC) and two considered as resistant (Bt and NO-QA) to B. thuringiens toxins under laboratory conditions. Susceptibility bioassays were performed on the three populations and toxins Cry1Ca and Cry2Ac, estimating lethal concentration (LC50) and its sublethal effects. Cry2Ac toxin did not cause mortality on the caterpillars from the three populations, so the sublethal tests were performed only with Cry1Ca toxin. The biological parameters evaluated were: pupal period and weight, caterpillar and pupal survival, male and female longevity and fecundity. The populations showed susceptibilities to Cry1Ca toxin, with statistically equal behavio... (Complete abstract click electronic access below) / Mestre

Coronal Mass Ejections (CMEs) are considered to be one of the most energetic events in the heliosphere. Capable of inducing geomagnetic storms on Earth that can cause damage to electronics, a pillar which the modern society we live in leans heavily upon. Being able to accurately predict the arrival of CMEs would present us with the ability to issue timely warnings to authorities and commercial actors, allowing for protective measures to be put in place minimizing the damage. In this study the predicted arrival times and speeds from the Drag Based Model (DBM) and Drag Based Ensemble Model (DBEM) were compared to observational data from a set of 12 events containing fast, Earth-directed Halo CMEs and their corresponding shocks. Although DBM was developed to model CME propagation, varying some parameters allow it to be used for estimating shock/sheath arrival. The results presented in this study indicate that on average DBM performs best when the drag-parameter γ is in the range 0.2 ≤ γ ≤ 0.3. However the variability in the results show that determining a universal value of γ for fast CMEs does not increase the consistency in the model's performance. For completeness, further investigation is needed to account for not only halo CMEs. This will allow to test broader range of variation in the DBEM input parameters.