<p>Studies of hybrid zones offer important insights into the process of speciation. Much of the knowledge to be gained is dependent on an accurate estimation of the strength of pre- and post-zygotic isolation between hybridizing taxa. My results demonstrate that hybridization can variously affect different components of fitness. In Ficedula flycatchers, late-breeding females may directly benefit from pairing with a heterospecific male by gaining access to superior territories. The hybrid offspring possess an immune system that is as equally well functioning as in the parental species (the collared, F. albicollis, and pied flycatcher, F. hypoleuca). However, I found that a severe reduction in fertility persists for at least three generations after the actual hybridization event. Combining all information about the reproductive success of hybridizing individuals and their descendents revealed that postzygotic isolation between flycatchers is very strong; hybridizing individuals leave almost no descendents. This thesis presents one of few comprehensive summaries of the selection for/against assortative mating in a natural hybrid zone. These findings suggest a central role for intrinsic postzygotic isolation as a reproductive barrier separating newly evolved bird species, and contrast previous suggestions that postmating isolation is the slowest of the reproductive barriers to evolve in birds.</p><p>Despite this strong selection against hybridization, pre-mating isolation is incomplete. Hybridization often results from females lacking conspecific partners, but appears to be also caused by errors in species recognition. Much of this error probably reflects the short period of time that pied flycatchers on Gotland and Öland have been in sympatry. Compared to collared flycatchers, pied flycatchers are poorer able to discriminate between conspecific and heterospecific song, and male pied flycatchers more often falsely signal their own identity through heterospecific song copying. However, despite colonising the study site from other sympatric populations and having very little gene flow from allopatry, collared flycatchers also possess traits (e.g. delayed plumage maturation) that increase their hybridization risk. Once pre-mating isolation is strong, the rarity of hybridization probably inhibits further selection against traits promoting interspecific mating, especially when such traits may be beneficial in other contexts. This thesis highlights complex interactions between factors affecting hybridization rate that would not be detected if such a study were not field-based. Furthermore, it showcases likely examples in nature of a number of theoretical objections to the evolution of pre-mating barriers between populations living in sympatry.</p>

A study of nuclei with few nucleons outside the closed shell provides benchmarks for the nuclear shell model especially in this modern era of physics where exotic doubly magic nuclei can be tested. The subject of this thesis is to experimentally investigate the properties of nuclei near 78Ni and to confront them with the predictions of modern large scale shell model calculations. In this regard, an experiment was performed at the National Superconducting Cyclotron Laboratory (NSCL) to measure excited states in 71-73Ni populated via the beta-decay of 71-73Co. Data collected from this experiment lead to partial level schemes for 71Ni and 73Ni and to improvements of existing level schemes for 70Ni and 72Ni. An objective of this experiment was to investigate the changes in excitation energy of the 5/2- state relative to the 9/2+ ground state as well as the search for the 1/2- isomeric state in odd-mass nickel isotopes approaching 78Ni. A second experiment was performed also at the NSCL where a two nucleon removal reaction from 73Cu was used to populate the low lying yrast states in 71Ni. Results from this in-beam experiment aided in constructing the level scheme of 71Ni. Systematics in shell model calculations using realistic interactions for odd mass 69-77Ni reveal a steady increase in energy spacing between the 1/2- level and 9/2+ ground state - suggesting an increased role of the g9/2 correlations, but an almost constant energy separation between the 5/2- and 1/2- excited states. Using data from the two experiments, the position of the 5/2- state in 71Ni and 73Ni and 1/2- state in 71Ni were identified. The decay of 74,76Ni into 74,76Cu was also investigated. Low lying states revealed new level schemes which are presented with an interpretation of the position of 1+ states populated via allowed GT transitions. A second project in this thesis is on the design and development of a detector system using a dual micro channel plate configuration. The system was built to detect and measure nanosecond isomers in neutron rich 73-76Cu and 76Zn isotopes. The design of the instrument and preliminary beam and alpha source tests done on the system are discussed.

Studies of hybrid zones offer important insights into the process of speciation. Much of the knowledge to be gained is dependent on an accurate estimation of the strength of pre- and post-zygotic isolation between hybridizing taxa. My results demonstrate that hybridization can variously affect different components of fitness. In Ficedula flycatchers, late-breeding females may directly benefit from pairing with a heterospecific male by gaining access to superior territories. The hybrid offspring possess an immune system that is as equally well functioning as in the parental species (the collared, F. albicollis, and pied flycatcher, F. hypoleuca). However, I found that a severe reduction in fertility persists for at least three generations after the actual hybridization event. Combining all information about the reproductive success of hybridizing individuals and their descendents revealed that postzygotic isolation between flycatchers is very strong; hybridizing individuals leave almost no descendents. This thesis presents one of few comprehensive summaries of the selection for/against assortative mating in a natural hybrid zone. These findings suggest a central role for intrinsic postzygotic isolation as a reproductive barrier separating newly evolved bird species, and contrast previous suggestions that postmating isolation is the slowest of the reproductive barriers to evolve in birds. Despite this strong selection against hybridization, pre-mating isolation is incomplete. Hybridization often results from females lacking conspecific partners, but appears to be also caused by errors in species recognition. Much of this error probably reflects the short period of time that pied flycatchers on Gotland and Öland have been in sympatry. Compared to collared flycatchers, pied flycatchers are poorer able to discriminate between conspecific and heterospecific song, and male pied flycatchers more often falsely signal their own identity through heterospecific song copying. However, despite colonising the study site from other sympatric populations and having very little gene flow from allopatry, collared flycatchers also possess traits (e.g. delayed plumage maturation) that increase their hybridization risk. Once pre-mating isolation is strong, the rarity of hybridization probably inhibits further selection against traits promoting interspecific mating, especially when such traits may be beneficial in other contexts. This thesis highlights complex interactions between factors affecting hybridization rate that would not be detected if such a study were not field-based. Furthermore, it showcases likely examples in nature of a number of theoretical objections to the evolution of pre-mating barriers between populations living in sympatry.

Enhanced Data Rates for Global Evolution (EDGE) is one of the 3G wireless communication standards, which provides higher data rates by adopting 8-PSK modulation in TDMA system infrastructure of GSM. In this thesis, a turbo detection receiver for Enhanced General Packet Radio Services (EGPRS) system, which is the packet switching mode of EDGE, is studied. In turbo detection, equalization and channel decoding are performed iteratively. Due to 8-ary alphabet of EGPRS modulation, full state trellis based equalization, as usually performed in GSM, is too complex not only for turbo detection but even for conventional equalization / so suboptimum schemes have to be considered. The Delayed Decision Feedback Sequence Estimation (DDFSE) is chosen as suboptimal and less complex trellis based scheme and it is examined as a conventional equalization technique firstly. It is shown that the DDFSE has a fine tradeoff between performance and complexity and can be a promising candidate for EGPRS. Then it is employed to reduce the number of the trellis state in turbo detection. Max-log-MAP algorithm is used for soft output calculations of both SISO equalizer and SISO decoder. Simulation results illustrate that proposed turbo detection structure improves bit error rate and block error rate performance of the receiver with respect to the conventional equalization and decoding scheme. The iteration gain varies depending on modulation type and coding rate of Modulation Coding Scheme (MCS) employed in EGPRS.

Probabilistic assessment and life cycle management of engineering components and systems in a nuclear power plant is intended to ensure safe and efficient operation of energy generation over its entire life. The CANDU reactor core consists of 380-480 pressure tubes, which are like miniature pressure vessels that contain natural uranium fuel. Pressure tubes operate under severe temperature and radiation conditions, which result in degradation with ageing. Presence of flaws in a pressure tube makes it vulnerable to delayed hydride cracking (DHC), which may lead to rupture or break-before-leak situation. Therefore, assessment of flaws in the pressure tubes is considered an integral part of a reactor core assessment program. The main objective of the thesis is to develop advanced probabilistic and mechanical stress field models for the assessment of flaws. The flaw assessment models used by the industries are based on deterministic upper/lower bound values for the variables and they ignore uncertainties associated with system parameters. In this thesis, explicit limit state equations are formulated and first order reliability method is employed for reliability computation, which is more efficient than simulation-based methods. A semi-probabilistic approach is adopted to develop an assessment model, which consists of a mechanics-based condition (or equation) involving partial factors that are calibrated to a specified reliability level. This approach is applied to develop models for DHC initiation and leak-before-break assessments. A novel feature of the proposed method is that it bridges the gap between a simple deterministic analysis and complex simulations, and it is amenable to practical applications. The nuclear power plant systems are not easily accessible for inspection and data collection due to exposure to high radiation. For this reason, small samples of pressure tubes are inspected at periodic intervals and small sample of data so collected are used as input to probabilistic analysis. The pressure tube flaw assessment is therefore confounded by large sampling uncertainties. Therefore, determination of adequate sample size is an important issue. In this thesis, a risk informed approach is proposed to define sample size requirement for flaw assessment. Notch-tip stress field is a key factor in any flaw assessment model. Traditionally, linear elastic fracture mechanics (LEFM) and its extension, serves the basis for determination of notch-tip stress field for elastic and elastic-perfectly-plastic material, respectively. However, the LEFM solution is based on small deformation theory and fixed crack geometry, which leads to singular stress and strain field at the crack-tip. The thesis presents new models for notch and crack induced stress fields based on the deformed geometry. In contrast with the classical solution based on small deformation theory, the proposed model uses the Cauchy's stress definition and boundary conditions which are coupled with the deformed geometry. This formulation also incorporates the rotation near the crack-tip, which leads to blunting and displacement of the crack-tip. The solution obtained based on the final deformed configuration yields a non-singular stress field at the crack-tip and a non-linear variation of stress concentration factor for both elastic and elastic-perfectly-plastic material. The proposed stress field formulation approach is applied to formulate an analytical model for estimating the threshold stress intensity factor (KIH) for DHC initiation. The analytical approach provides a relationship between KIH and temperature that is consistent with experimental results.

Probabilistic assessment and life cycle management of engineering components and systems in a nuclear power plant is intended to ensure safe and efficient operation of energy generation over its entire life. The CANDU reactor core consists of 380-480 pressure tubes, which are like miniature pressure vessels that contain natural uranium fuel. Pressure tubes operate under severe temperature and radiation conditions, which result in degradation with ageing. Presence of flaws in a pressure tube makes it vulnerable to delayed hydride cracking (DHC), which may lead to rupture or break-before-leak situation. Therefore, assessment of flaws in the pressure tubes is considered an integral part of a reactor core assessment program. The main objective of the thesis is to develop advanced probabilistic and mechanical stress field models for the assessment of flaws. The flaw assessment models used by the industries are based on deterministic upper/lower bound values for the variables and they ignore uncertainties associated with system parameters. In this thesis, explicit limit state equations are formulated and first order reliability method is employed for reliability computation, which is more efficient than simulation-based methods. A semi-probabilistic approach is adopted to develop an assessment model, which consists of a mechanics-based condition (or equation) involving partial factors that are calibrated to a specified reliability level. This approach is applied to develop models for DHC initiation and leak-before-break assessments. A novel feature of the proposed method is that it bridges the gap between a simple deterministic analysis and complex simulations, and it is amenable to practical applications. The nuclear power plant systems are not easily accessible for inspection and data collection due to exposure to high radiation. For this reason, small samples of pressure tubes are inspected at periodic intervals and small sample of data so collected are used as input to probabilistic analysis. The pressure tube flaw assessment is therefore confounded by large sampling uncertainties. Therefore, determination of adequate sample size is an important issue. In this thesis, a risk informed approach is proposed to define sample size requirement for flaw assessment. Notch-tip stress field is a key factor in any flaw assessment model. Traditionally, linear elastic fracture mechanics (LEFM) and its extension, serves the basis for determination of notch-tip stress field for elastic and elastic-perfectly-plastic material, respectively. However, the LEFM solution is based on small deformation theory and fixed crack geometry, which leads to singular stress and strain field at the crack-tip. The thesis presents new models for notch and crack induced stress fields based on the deformed geometry. In contrast with the classical solution based on small deformation theory, the proposed model uses the Cauchy's stress definition and boundary conditions which are coupled with the deformed geometry. This formulation also incorporates the rotation near the crack-tip, which leads to blunting and displacement of the crack-tip. The solution obtained based on the final deformed configuration yields a non-singular stress field at the crack-tip and a non-linear variation of stress concentration factor for both elastic and elastic-perfectly-plastic material. The proposed stress field formulation approach is applied to formulate an analytical model for estimating the threshold stress intensity factor (KIH) for DHC initiation. The analytical approach provides a relationship between KIH and temperature that is consistent with experimental results.

In einem komplexen tierexperimentellen Ansatz wurde das Potenzial der anti huCD4-Antikörper MAX16H5 und MAX12F6 zur Modulierung zellvermittelter Immun-reaktionen in vivo untersucht. Dafür kam ein mehrfach transgenes Mausmodell zur Anwendung, in dem das humane Zielmolekül und dessen physiologischer Ligand als Transgene exprimiert waren. Als T-Zell vermittelte Immunreaktion wurde eine Kon-taktreaktion (delayed type hypersensitivity, DTH) gegen DNFB etabliert und validiert. An der DTH wurde untersucht, ob und wie die verschiedenen Antikörper die Sen-sibilisierungs- und die Auslösungsphase beeinflussen. Die experimentellen Ergeb-nisse zeigen, dass die Antikörper epitop- und isotypabhängig die beiden Phasen der DTH unterschiedlich beeinflussen. Die Applikation der Antikörper während der Sensi-bilisierung führte zu einer unterschiedlich ausgeprägten Suppression der DTH. Dage-gen hatten sie gegensätzliche Effekte auf die Auslösung. Während nach MAX12F6-Behandlung eine stärkere und prolongierte DTH gemessen wurde, verlief die DTH-Reaktion nach MAX16H5-Applikation deutlich abgeschwächt. Mittels flowzytometri-scher Analysen konnte gezeigt werden, dass die Antikörper unterschiedliche Subpo-pulationen der T-Helferzellen depletieren. Darüber hinaus führte MAX16H5 offen-sichtlich zur Induktion regulatorischer T-Zellen. Die Daten erklären unterschiedliche Erfolge aus ersten klinischen Studien mit verschiedenen anti huCD4 Antikörpern. Auch eignet sich CD4 auch als diagnostisches Target zur in vivo Diagnostik T-Zell vermittelter Entzündungsreaktionen. Mit Antikörperfragmenten von MAX16H5 wurde ein immunszintigraphisches Verfahren entwickelt, das die spezifische Darstellung der mit der DTH einhergehenden Entzündungsreaktion ermöglicht.

La tolérance orale permet la modulation de la réponse immunitaire à l’égard des antigènes exogènes présents dans la lumière intestinale. Essentiels à l’établissement d’une relation symbiotique entre le système immunitaire et la flore intestinale, l’induction et le maintien de la tolérance orale reposent sur différents mécanismes immunologiques. Parmi eux, l’induction de cellules T régulatrices par les cellules dendritiques et de mécanismes apoptotiques. Or, la glycoprotéine membranaire CD47 est impliquée, en périphérie, dans ces mécanismes. Cependant, le rôle de CD47 dans la tolérance orale n’est pas connu. À l’aide d’un modèle murin déficient en CD47, nous avons démontré principalement, que l’absence de CD47 est associée à une diminution de 50 % de la proportion de cellules dendrites myéloïdes CD11b+CD103- retrouvées dans les ganglions mésentériques. Suite au transfert adoptif de cellules T antigènes spécifiques dans nos différents modèles expérimentaux, on a, aussi, observé une diminution de 45 % de leur niveau d’activation dans les ganglions mésentériques. Malgré les effets observés, le CD47 n’est pas impliqué dans l’induction d’une réaction de tolérance orale secondaire à l’administration intragastrique de fortes doses d’ovalbumine. Cependant, nous avons démontré que CD47 est impliquée au niveau de la migration des cellules dendritiques de la peau et de certaines sous-populations retrouvées dans les ganglions mésentériques. / Oral tolerance allows the modulation of the immune response against exogenous antigens present in the intestinal lumen. Essential to establish a symbiotic relationship between the immune system and intestinal flora, the induction and maintenance of oral tolerance rests on different immunological mechanisms. Among them, induction of regulatory T cells by dendritic cells and apoptotic mechanisms. However, the membrane glycoprotein CD47 is involved in the periphery of these mechanisms. However, the role of CD47 in oral tolerance is unknown. With a mouse model deficient in CD47, we showed mainly that the absence of CD47 is associated with a decrease of 50% in the proportion of myeloid dendritic cells CD11b+ CD103- found in the mesenteric lymph nodes. Following the adoptive transfer of antigen specific T cells in our experimental models, we also observed a decrease of 45% of their level of activation in mesenteric lymph nodes. Despite the observed effects, CD47 is not involved in the induction of oral tolerance response secondary to intragastric administration of high doses of ovalbumin. However, we have shown that CD47 is involved in the migration of dendritic cells of the skin and some sub-populations found in mesenteric lymph nodes.

The main problem evaluated in this manuscript is the stabilization of periodic orbits of non-linear dynamical systems by use of feedback control. The goal of the control methods proposed in this work is to achieve a stable periodic oscillation. These control methods are applied to systems that present unstable periodic orbits in the state space, and the latter are the orbits to be stabilized.The methods proposed here are such that the resulting stable oscillation is obtained with low control effort, and the control signal is designed to converge to zero when the trajectory tends to the stabilized orbit. Local stability of the periodic orbits is analyzed by studying the stability of some linear time-periodic systems, using the Floquet stability theory. These linear systems are obtained by linearizing the trajectories in the vicinity of the periodic orbits.The control methods used for stabilization of periodic orbits here are the proportional feedback control, the delayed feedback control and the prediction-based feedback control. These methods are applied to discrete and continuous-time systems with the necessary modifications. The main contributions of the thesis are related to these methods, proposing an alternative control gain design, a new control law and related results.

L'étude des noyaux exotiques est un outil important pour sonder la structure nucléaire des noyaux éloignés de la stabilité. Pour des noyaux situés à la drip-line proton, l'émission directe de protons a été prédite depuis le début des années 1960. Ce travail de thèse est focalisé sur l'étude de la radioactivité 2-protons du noyau 45Fe. Un modèle théorique récent, dit modèle à trois corps, prévoit les corrélations énergétiques et angulaires qui doivent exister entre les protons émis. Afin d'étudier le processus d'émission, une Chambre à Projection Temporelle a spécialement été conçue pour reconstruire en trois dimensions la trajectoire des protons. Elle a été utilisée pendant une expérience sur le séparateur LISE3 du GANIL pour l'étude de 45Fe. Pour la première fois, les deux protons émis ont pu être détectés individuellement. Des outils d'analyse spécifiques ont permis de calculer les corrélations énergétiques entre les protons, montrant qu'ils partagent équitablement l'énergie disponible, tel que le prévoient les différents modèles théoriques. Les corrélations angulaires ont été étudiées, mais le manque de statistique ne permet pas de trancher définitivement sur le processus d'émission mis en jeu même si les données sont en accord avec les prévisions du modèle à trois corps. L'expérience a également permis d'étudier l'émission retardée de protons de 43Cr. L'émission beta-2p a été identifiée ; les corrélations énergétiques et angulaires sont en faveur d'une émission séquentielle des deux protons. Enfin, et pour la première fois, l'émission retardée de trois protons a été observée pour ce noyau.