<p>Climate and environmental change following the retreat of the last glacial ice sheet in the Antarctic Peninsula has been interpreted, employing diatom abundance, relative abundance of <i>Chaetoceros</i> resting spores and diatom assemblages as proxies. These together with sedimentological data and radiocarbon dating, suggest four major events that can be further subdivided.</p><p>Deglaciation ~13.2-11.5 kyr BP with ice shelf breakup and strong surface water stratification from melting ice. </p><p>Climate reversal ~11.5-9.0 kyr BP with turbulent water masses. </p><p>Climate optimum ~9.0-4.0 kyr BP with intrusions of northern ´warm` water masses. </p><p>Neoglacial ~4.0 kyr BP-present with extended periods of sea ice cover and increased storm frequency. </p><p>One aspect of climate change is the stability of marine based ice sheets, and the interaction with underlying sediment. A pilot study on characterizing sediment influenced by past ice streaming (Ross Sea) was performed using diatom, texture and chemical analysis. The results show that:</p><p>Diamictons are chemically and texturally well homogenized, whereas diatom assemblages suggest different degrees of stratigraphic mixing and reworking related to mode of glacial sediment transport. </p><p>Mud appears in different stratigraphic sections deposited in sub-ice shelf or ice edge environment, or through winnowing by currents. This is evident through stratigraphically-diverse diatom assemblages and texture. Most sediment characterized as mud is enriched in zinc (Zn). </p><p>Hemipelagic diatomaceous muds are enriched in barium (Ba) and the diatom assemblage is dominated by typical neritic post-glacial species.</p>

The Laurentian Great Lake, Lake Erie is an invaluable global resource and its watershed is home to over 11 million people. The pressures placed on the lake because of this high population caused Lake Erie to experience numerous environmental problems, including seasonal hypoxia and harmful algal blooms. While these topics have been widely studied in Lake Erie for over 40 years a more nuanced understanding of the interaction between phytoplankton and nutrient is needed to properly address the problems continuing to face the lake. In this study we combine classical limnological and cell growth experiments with modern molecular biological techniques and microscopy to more completely describe the aquatic microbial ecology of the lake. We used an oxalate rinse technique to examine the surface absorbed P pool of the toxic cyanobacterium Microcystis aeruginosa grown under a range of P conditions, as well as the general Lake Erie plankton assemblage. Our results suggest that while Microcystis is plastic in its cellular P needs, the ratio of intracellular to extracellular P remains stable across growth conditions. We describe the effect of the phosphonate herbicide glyphosate on the Lake Erie phytoplankton community using laboratory cell growth studies, field microcosm experiments and PCR amplification of a gene implicated in the breakdown of this compound from the environment. Results from these experiments suggest that the presence of glyphosate can affect community structure in multiple ways and may explain areas of unexplained phytoplankton diversity in coastal areas of Lake Erie. We also show heterotrophic bacteria are likely critical to the breakdown of glyphosate and further illustrate that understanding the context of the larger microbial community is critical to understanding the ecology of the constituent members of the community. Finally, we investigate the activity of the phytoplankton community in winter months with a focus on diatoms abundant in Lake Erie under the ice. We show these diatoms are active and that the winter bloom is a likely source of carbon important to seasonal hypoxia formation. Together, these studies significantly enrich our understanding of how phytoplankton influence important ecological processes in Lake Erie.

Climate and environmental change following the retreat of the last glacial ice sheet in the Antarctic Peninsula has been interpreted, employing diatom abundance, relative abundance of Chaetoceros resting spores and diatom assemblages as proxies. These together with sedimentological data and radiocarbon dating, suggest four major events that can be further subdivided. Deglaciation ~13.2-11.5 kyr BP with ice shelf breakup and strong surface water stratification from melting ice. Climate reversal ~11.5-9.0 kyr BP with turbulent water masses. Climate optimum ~9.0-4.0 kyr BP with intrusions of northern ´warm` water masses. Neoglacial ~4.0 kyr BP-present with extended periods of sea ice cover and increased storm frequency. One aspect of climate change is the stability of marine based ice sheets, and the interaction with underlying sediment. A pilot study on characterizing sediment influenced by past ice streaming (Ross Sea) was performed using diatom, texture and chemical analysis. The results show that: Diamictons are chemically and texturally well homogenized, whereas diatom assemblages suggest different degrees of stratigraphic mixing and reworking related to mode of glacial sediment transport. Mud appears in different stratigraphic sections deposited in sub-ice shelf or ice edge environment, or through winnowing by currents. This is evident through stratigraphically-diverse diatom assemblages and texture. Most sediment characterized as mud is enriched in zinc (Zn). Hemipelagic diatomaceous muds are enriched in barium (Ba) and the diatom assemblage is dominated by typical neritic post-glacial species.

Aquesta tesi tracta la jerarquia i l'heterogeneïtat dels sistemes fluvials que afecten l'estructura de les comunitats bentòniques de diatomees. A nivell regional, es van buscar diferents grups de punts i les seves espècies indicadores, es va estudiar la resposta de les comunitats de diatomees als gradients ambientals, es va avaluar la utilitat de diferents índexs de diatomees i es va buscar el millor sistema de classificació per a condicions de referència. A nivell de conca, es volien definir els factors que determinen la distribució longitudinal de la diversitat de les comunitats de diatomees. Finalment, a nivell d'hàbitat es van determinar quins factors afecten les algues i els cianobacteris a aquesta escala i es va examinar la contribució relativa de l'ambient i l'espai en la distribució de la biomassa i composició d'algues i cianobacteris. Per tant, els diferents capítols d'aquesta tesi han estat desenvolupats seguint aquest esquema. / This thesis deals with the hierarchy and heterogeneity of stream systems affecting the structure of benthic diatom communities. At a regional level, I search for different groups of sites and their indicator taxa, I studied the responses of the diatom communities to the gradients of environmental variables, I tested the usefulness of diatom indices and I searched for the best classification system for reference conditions. At a watershed level my interest was to define the factors that determined the longitudinal distribution of diversity of diatom communities. Finally, at a habitat level it was interesting to determine the factors affecting algae and cyanobacteria at this scale and examine the relative effects of environmental factors and space on the distribution of biomass and composition of benthic algae and cyanobacteria. Thus, the different chapters of the thesis had been approached following this scheme.

Lake Malawi is the second largest lake in Africa, supporting diverse populations of endemic cichlids and supplying essential water resources to Malawi, Mozambique and Tanzania. However, population growth, rapid deforestation and intensive agriculture, especially in the southern catchments, have accelerated soil erosion during the last half century. These anthropogenically-disturbed catchments have caused rivers to transport greater sediment loads into Lake Malawi than rivers within forested catchments. Lake Malawi?s immense size and oligotrophic nature may retard detection of inputs of external contaminants. Reversing the effects of increased nutrient loading to Lake Malawi once observed would likely take generations, as the residence time of water is over 140 years. Therefore, sensitive metrics are required to assess the effects of land use change and climate variability in Lake Malawi in advance of deleterious effects. In this study, paleolimnological analyses of four sediment cores collected in 1997 and 1998 along a longitudinal transect of Lake Malawi, dated with ²¹⁰Pb analyses and analyzed for biogenic silica and sedimentary diatom assemblages, were used to create a long-term water quality dataset. These four sites span gradients of land use and latitude in order to reconstruct limnological conditions over the whole lake during the last 300 years. Paleoecological results indicate that patterns of diatom assemblage change are not uniform lake wide. Southern cores contain evidence of nutrient enrichment starting as early as ca. 1940, indicated by increased silica, carbon and nitrogen burial. By ca. 1970, increased rates of sedimentation, diatom influx and changes in diatom community composition, characterized by increased percent abundance of eutrophic diatom taxa, are attributable to accelerated enrichment by terrestrial soil erosion. The succession of diatoms in southern Lake Malawi begins with high percent abundance of <i>Aulacoseira nyassensis</i> and <i>Fragilaria africana</i>, which thrive in nutrient-rich waters, followed by a shift towards diatom taxa with reduced silica requirements by ca. 1980 (e. g. <i>Stephanodiscus nyassae, S. minutulus, S. muelleri, Cyclostephanos</i> and small <i>Nitzschia</i> species. ), a pattern comparable to the eutrophication-induced decline in silica to phosphorus ratios in Lake Victoria. In Lake Malawi, evidence of eutrophication extends to the mid lake as indicated by similar diatom assemblage changes in the sediment core from the central region. Diatom stratigraphies from the north end of the lake indicate no observable impacts of land use change on the northern basin of Lake Malawi during the past 350 years. However, a nine-meter rise in water level ca. 1860 AD appears to have resulted in elevated diatom influxes at that time comparable to the recent eutrophication-induced diatom influxes of the southern cores. The effects of this rise in water level was recorded in all three measured sites, southern, central and northern Lake Malawi, indicating lake-wide increased productivity, yet changes to the diatom community composition were imperceptible. This study shows evidence of recent cultural eutrophication altering limnological conditions with impacts to the biogeochemical cycling of silica, the available silica to phosphorus ratios and the biotic communities of a large portion of Lake Malawi. Thus, providing an early warning that proper stewardship of Lake Malawi requires effective management of land-use practices within the catchment to reduce soil erosion and avoid widespread water quality deterioration of this great lake.

Lake Malawi is the second largest lake in Africa, supporting diverse populations of endemic cichlids and supplying essential water resources to Malawi, Mozambique and Tanzania. However, population growth, rapid deforestation and intensive agriculture, especially in the southern catchments, have accelerated soil erosion during the last half century. These anthropogenically-disturbed catchments have caused rivers to transport greater sediment loads into Lake Malawi than rivers within forested catchments. Lake Malawi?s immense size and oligotrophic nature may retard detection of inputs of external contaminants. Reversing the effects of increased nutrient loading to Lake Malawi once observed would likely take generations, as the residence time of water is over 140 years. Therefore, sensitive metrics are required to assess the effects of land use change and climate variability in Lake Malawi in advance of deleterious effects. In this study, paleolimnological analyses of four sediment cores collected in 1997 and 1998 along a longitudinal transect of Lake Malawi, dated with ²¹⁰Pb analyses and analyzed for biogenic silica and sedimentary diatom assemblages, were used to create a long-term water quality dataset. These four sites span gradients of land use and latitude in order to reconstruct limnological conditions over the whole lake during the last 300 years. Paleoecological results indicate that patterns of diatom assemblage change are not uniform lake wide. Southern cores contain evidence of nutrient enrichment starting as early as ca. 1940, indicated by increased silica, carbon and nitrogen burial. By ca. 1970, increased rates of sedimentation, diatom influx and changes in diatom community composition, characterized by increased percent abundance of eutrophic diatom taxa, are attributable to accelerated enrichment by terrestrial soil erosion. The succession of diatoms in southern Lake Malawi begins with high percent abundance of <i>Aulacoseira nyassensis</i> and <i>Fragilaria africana</i>, which thrive in nutrient-rich waters, followed by a shift towards diatom taxa with reduced silica requirements by ca. 1980 (e. g. <i>Stephanodiscus nyassae, S. minutulus, S. muelleri, Cyclostephanos</i> and small <i>Nitzschia</i> species. ), a pattern comparable to the eutrophication-induced decline in silica to phosphorus ratios in Lake Victoria. In Lake Malawi, evidence of eutrophication extends to the mid lake as indicated by similar diatom assemblage changes in the sediment core from the central region. Diatom stratigraphies from the north end of the lake indicate no observable impacts of land use change on the northern basin of Lake Malawi during the past 350 years. However, a nine-meter rise in water level ca. 1860 AD appears to have resulted in elevated diatom influxes at that time comparable to the recent eutrophication-induced diatom influxes of the southern cores. The effects of this rise in water level was recorded in all three measured sites, southern, central and northern Lake Malawi, indicating lake-wide increased productivity, yet changes to the diatom community composition were imperceptible. This study shows evidence of recent cultural eutrophication altering limnological conditions with impacts to the biogeochemical cycling of silica, the available silica to phosphorus ratios and the biotic communities of a large portion of Lake Malawi. Thus, providing an early warning that proper stewardship of Lake Malawi requires effective management of land-use practices within the catchment to reduce soil erosion and avoid widespread water quality deterioration of this great lake.

In the subtropical oligotrophic ocean, nitrogen fixation is an important source of new nitrogen (N) for supporting biological production. Previous studies have found that nitrogen-fixing Diatom-Diazotroph Associations (DDAs) are in high abundance in the intermediate salinity zone of large river plumes such as the Amazon and Mekong rivers, while Trichodesmium spp. becomes more abundant at higher salinities. This recurring pattern in the Amazon River plume suggests that strong salinity and nutrient gradients within the river plumes may lead to a cascade in diazotroph communities. I hypothesized that the Mississippi River, a major source of freshwater, nutrients and sediments to the northern Gulf of Mexico, creates a similar distribution of diazotroph communities. The relationship between large diazotrophs and salinity was examined in samples collected in July 2011 during a flood outflow from the Mississippi River. The dominant DDA, Hemiaulus spp. – Richelia spp., was at greatest abundance (≈31,000 cells L-1) west of the birdfoot delta on the periphery of the plume (≈29 salinity) where bottom water hypoxia was also observed. Trichodesmium spp., a cyanobacterium genus that occurs in both colonial and free trichome morphologies, was abundant at both high (≈35) salinities east of the delta reaching 20+ colonies L-1, as well as in the fresher (≈28) waters of the plume where it reached 3,500 trichomes L-1. Diazotroph distributions were separated east and west of the Mississippi River outflow, with DDAs being most abundant over bottom water hypoxic regions to the west and Trichodesmium spp. in high abundance to the east. The diazotroph – salinity gradient relationships present within the Amazon River plume were not present within the Gulf of Mexico. This study suggests that environmental factors other than salinity, such as nutrients or hypoxia, are influencing the distribution of diazotrophs around the Mississippi River plume. The seasonal hypoxia seen in the Gulf of Mexico with the co-occurring DDA increase could appear in other river systems. / text

Five different forms of ribulose 1,5-bisphosphate carboxylase/oxygenase (RubisCO; IA, IB, IC, ID, II), the carboxylase of the Calvin-Benson-Bassham cycle (CBB), are utilized by plants, algae and autotrophic bacteria for carbon fixation. Discrimination against 13C by RubisCO is a major factor dictating the stable carbon isotopic composition (δ13C = {[13C/12C sample/13C/12C standard] - 1} X 1000) of biomass. To date, isotope discrimination, expressed as ε values (={[12k/13k] - 1} X 1000; 12k and 13k = rates of 12C and 13C fixation) has been measured for form IA, IB, and II RubisCOs from only a few species, with ε values ranging from 18 to 29 /. The aim of this study was to better characterize form ID and IC RubisCO enzymes, which differ substantially in primary structure from the IB enzymes present in many cyanobacteria and organisms with green plastids, by measuring isotopic discrimination and kinetic parameters (KCO2 and Vmax). Several major oceanic primary producers, including diatoms, coccolithophores, and some dinoflagellates have form ID RubisCO, while form IC RubisCO is present in many proteobacteria of ecological interest, including marine manganese-oxidizing bacteria, some nitrifying and nitrogen-fixing bacteria, and extremely metabolically versatile organisms such as Rhodobacter sphaeroides. The ε - values of the form ID RubisCO from the coccolithophore, Emiliania huxleyi and the diatom, Skeletonema costatum (respectively 11.1 / and 18.5 /) were measured along with form IC RubisCO from Rhodobacter sphaeroides and Ralstonia eutropha (respectively 22.9 / and 19.0 /). Isotopic discrimination by these form ID/IC RubisCOs is low when compared to form IA/IB RubisCOs (22-29 /). Since the measured form ID RubisCOs are less selective against 13C, oceanic carbon cycle models based on 13C values may need to be reevaluated to accommodate lower ε values of RubisCOs found in major marine algae. Additionally, with further isotopic studies, the extent to which form IC RubisCO from soil microorganisms contributes to the terrestrial carbon sink may also be determined.

abstract: Biogenic silica nanostructures, derived from diatoms, possess highly ordered porous hierarchical nanostructures and afford flexibility in design in large part due to the availability of a great variety of shapes, sizes, and symmetries. These advantages have been exploited for study of transport phenomena of ions and molecules towards the goal of developing ultrasensitive and selective filters and biosensors. Diatom frustules give researchers many inspiration and ideas for the design and production of novel nanostructured materials. In this doctoral research will focus on the following three aspects of biogenic silica: 1) Using diatom frustule as protein sensor. 2) Using diatom nanostructures as template to fabricate nano metal materials. 3) Using diatom nanostructures to fabricate hybrid platform. Nanoscale confinement biogenetic silica template-based electrical biosensor assay offers the user the ability to detect and quantify the biomolecules. Diatoms have been demonstrated as part of a sensor. The sensor works on the principle of electrochemical impedance spectroscopy. When specific protein biomarkers from a test sample bind to corresponding antibodies conjugated to the surface of the gold surface at the base of each nanowell, a perturbation of electrical double layer occurs resulting in a change in the impedance. Diatoms are also a new source of inspiration for the design and fabrication of nanostructured materials. Template-directed deposition within cylindrical nanopores of a porous membrane represents an attractive and reproducible approach for preparing metal nanopatterns or nanorods of a variety of aspect ratios. The nanopatterns fabricated from diatom have the potential of the metal-enhanced fluorescence to detect dye-conjugated molecules. Another approach presents a platform integrating biogenic silica nanostructures with micromachined silicon substrates in a micro/nano hybrid device. In this study, one can take advantages of the unique properties of a marine diatom that exhibits nanopores on the order of 40 nm in diameter and a hierarchical structure. This device can be used to several applications, such as nano particles separation and detection. This platform is also a good substrate to study cell growth that one can observe the reaction of cell growing on the nanostructure of frustule. / Dissertation/Thesis / Doctoral Dissertation Materials Science and Engineering 2014

O estudo de três testemunhos rasos (até 2,5m de profundidade) coletados na região de Jaguaruna, litoral centro-sul de Santa Catarina, permitiu reconstituir parte da história de evolução sedimentar holocênica na área. Para isso, foi feita a análise integrada de quatro tipos de variáveis: sedimentológicas, diatomológicas, palinológicas e geoquímicas (Ctotal, Ntotal, 13C e 15N). Os dados de micropaleontologia foram tratados por métodos de estatística mutivariada (análise fatorial de correspondência, análise de componentes principais e classificação ascendente hierárquica), que evidenciaram agrupamentos significativos entre os microfósseis e, desse modo, auxiliaram nas interpretações. A ca. 5000 anos AP, a área de estudo deveria ser ocupada por um conjunto de lagunas interconectadas, cuja existência pôde ser atestada pelos sedimentos de fundo lagunar, na base de um dos testemunhos, em zona atualmente colonizada por mata de restinga. A ligação da laguna com o mar, nesta época, é verificada pela presença de diatomáceas marinhas e pelo sinal isotópico e elementar da matéria orgânica preservada nos sedimentos, indicativo de origem algácea, com valores de 13C de fitoplâncton marinho. A perda da conexão da laguna com o oceano ocorreu em diferentes momentos nos três testemunhos, sendo o último registro de desconexão da laguna observado a ca. 2740-2370 anos cal AP. Variações nas assembléis de diatomáceas no registro da fase lagunar (de 5500 até 2740-2370 anos cal AP) indicaram pelo menos dois períodos de mudanças maiores na salinidade da paleolaguna. Estes períodos puderam ser comparados a dados de paleoprecipitação, publicados em trabalhos anteriores, obtidos para o Estado de Santa Catarina. Observou-se boa correlação entre períodos com maior precipitação e períodos de diminuição na salinidade das águas da laguna, o que seria relacionado à sua diluição por maior aporte fluvial, sem excluir, no entanto, a possibilidade de menor influência marinha devido a dinâmica de abertura e fechamento das conexões da laguna com o oceano. O registro da vegetação através da analise palinológica auxiliou na elaboração do modelo evolutivo da área e permitiu reconstituir parte da história da vegetação no contexto de preenchimento da bacia lagunar. O desenvolvimento da vegetação de restinga em áreas antes ocupadas por vegetação característica de borda lagunar reforçaria a idéia de progradação das fácies costeiras assoreando por completo a paleolaguna na área. A síntese dos dados palinológicos entre os três testemunhos permite interpretar que, pelo menos desde 4000 anos cal AP, a vegetação na área de estudo não sofreu grandes modificações, o que sugere clima semelhante ao presente. A análise integrada dos diferentes indicadores paloambientais sugere que a dinâmica sedimentar deve ser a maior responsável pelas mudanças observadas nos três testemunhos, com evolução dos ecossistemas ligada, principalmente, a variações no regime e substrato deposicionais, relacionadas à progradação costeira. / Sediments from three cores collected in Jaguaruna (south Brazilian coast, Santa Catarina state) were analyzed, in order to reconstruct the Holocene sedimentary evolution of the area. The palaeoenvironmental reconstruction was based on microfossil proxies (diatoms, pollen and spores), analysis of sedimentary organic matter (13C, 15N and C/N ratios), and sedimentological analysis. From at least 5000 cal yr AP, the site was occupied by a system of interconnected lagoons, whose existence is verified in the core sediments by the record of paleolagoon sediments in area occupied by restinga forest nowadays. The existence of this lagoon and of its connection with the sea is indicated by the presence of marine diatoms and by the isotopic and elementary composition of the sedimentary organic matter, which is indicative of algal origin, with 13C values of marine phytoplankton. The end of the connection between sea and lagoon was registered in different moments in the three cores and the last record of disconnection is found in ca. 2740-2370 cal yr AP. The variations of the diatom assemblages from the lagoonal phase (between 5000 and 2740-2370 cal yr AP) indicated at least two periods of major changes in the paleolagoon salinity. These periods could be compared with the published paleoprecipitation data for the Santa Catarina state, which showed a good correlation between higher precipitation and reduction in the lagoon water salinity. This correlation would be related to dilution of water salinity by the greater fluvial discharges. However, it can not be exclude the possibility of a lower sea influence related with the dynamics of opening and closing connections between lagoon and ocean. The pollen analysis allowed the reconstruction of part of the vegetation history in the context of lagoon basin sedimentary filling. The development of the restinga forest in areas previously colonized by open vegetation around the lagoon suggested the progradation of coastal facies that completely filled paleolagoon in the area. The synthesis of the palinological data showed that no major changes of the mainland vegetation ecosystem took place at least since 4000 cal yr AP. The vegetation in the study area did not suffer great modifications; with no record of climatic changes. The multi-proxies analysis suggests that the sedimentary dynamics must be responsible for the greater changes observed in the core sediments. The evolution of ecosystems could be related to variations of the depositional process and modification in the character of the substratum, related to the coastal progradation.