Made available in DSpace on 2014-06-11T19:26:40Z (GMT). No. of bitstreams: 0 Previous issue date: 2011-02-21Bitstream added on 2014-06-13T18:54:56Z : No. of bitstreams: 1 janani_jk_me_botfca.pdf: 939751 bytes, checksum: 44d9b0cf69e527a9491a6a09a3dc8d50 (MD5) / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / A Lippia alba (Mill.) N.E.Br. ex Britt & Wilson é uma importante espécie medicinal de uso popular conhecida como erva cidreira brasileira. Trata-se de um arbusto, cujas folhas frescas ou secas e inflorescências são utilizadas na forma de chás, macerados, compressas, pomadas, banhos e extratos alcoólicos. Suas folhas apresentam ação antiespasmódica, moluscicida, fungicida, calmante e digestiva. Considerando, a) a inexistência de estudos sobre a L. alba em hidroponia b) a utilização de solução nutritiva para atender as necessidades da espécie, fornecendo nutrientes de maneira balanceada e possibilitando a produção de plantas com adequada qualidade e em menor espaço de tempo, c) a necessidade de produção de matéria prima para uso medicinal popular e farmacêutico, o presente estudo objetivou avaliar o desenvolvimento e as trocas gasosas de L. alba submetida à variação das concentrações dos nutrientes a partir da diluição da solução nutritiva de Hoagland e Arnon (1950). Assim, as plantas foram cultivadas em quatro tratamentos, constituídos pela variação de nutrientes na solução nutritiva n°2 de Hoagland e Arnon (1950), ou seja, em solução completa (100%) e diluída à 80%, 60% e 40% em relação à completa. As variáveis avaliadas foram área foliar, massas secas dos diferentes órgãos, razão de área foliar (RAF), área foliar específica (AFE), razão de massa foliar (RMF), taxa de crescimento absoluto (TCA), taxa assimilatória líquida (TAL), taxa de crescimento relativo (TCR), taxa de assimilação de CO2 (A, mmolCO2 m-2 s-1), condutância estomática (gs, mol m-2s-1), transpiração (E, mmol H2O m-2 s-1), concentração intercelular de CO2 na folha (Ci, mmolCO2 mol-1ar) e eficiência de uso da água (EUA). Os resultados obtidos permitem concluir que: 1. As concentrações dos nutrientes na solução nutritiva, de maneira geral, foram excessivas para... / Lippia alba (Mill.) N.E.Br. ex Britt & Wilson is a native species popular known as “erva-cidreira brasileira”. It is a shrub who’s fresh or dried leaves and flowers are used as teas, macerated, bandages, ointments, baths and alcoholic extracts. Its leaves have an antispasmodic, molluscicidal, fungicidal, sedative and digestive. Considering a) the lack of studies about L. alba in hydroponic cultivation b) the use of nutrient solution to meet the needs of the species, providing nutrients in a balanced manner and enabling the production of plants with adequate quality and in shortest time, c) the need for production of raw materials for folk medical and pharmacist, the present study evaluate the development and gas exchange of L. alba subjected to varying concentrations of nutrients from the dilution of Hoagland and Arnon (1950). Thus, plants were grown in four treatments, consisting by variation of nutrients in the nutrient solution No 2 of Hoagland and Arnon (1950), full solution (100%), diluted to 80%, 60% and 40% compared to the full. Variables evaluated were leaf area, dry masses of different organs, leaf area ratio (LAR), specific leaf area (SLA), leaf mass ratio (LMR), absolute growth rate (AGR), net assimilation rate (NAR), rate relative growth (RGR), rate of CO2 assimilation (A, molCO2 m m-2 s-1), stomatal conductance (gs, mol m-2s-1), transpiration (E, mmol H2O m-2 s -1), intercellular CO2 concentration in the leaf (Ci, molCO2 m mol-1AR) and efficiency of water use (USA). The results showed that: 1. The concentrations of nutrients in the nutrient solution, in general, were excessive for the development and gas exchange of Lippia alba, 2. The cultivation of the species with a dilute solution of 80% resulted in higher total dry matter in the shortest time possible, which, however, was highest among the various treatments, 3. Diluted solutions at 60 and 40% ...(Complete abstract click electronic access below)

Made available in DSpace on 2014-06-11T19:32:58Z (GMT). No. of bitstreams: 0 Previous issue date: 2010-03-26Bitstream added on 2014-06-13T21:05:28Z : No. of bitstreams: 1 lupatini_gc_dr_botfmvz.pdf: 341308 bytes, checksum: df486ae1605629080494d4b067f0a6e2 (MD5) / Universidade Estadual Paulista (UNESP) / O experimento foi conduzido na UNESP – Campus de Botucatu, Faculdade de Medicina Veterinária e Zootecnia. O delineamento experimental foi em blocos ao acaso num arranjo fatorial com três cultivares de Brachiaria brizantha (Marandu, Xaraés e BRS Piatã) e duas alturas de resíduo (15 e 25 cm), no total de seis tratamentos com quatro repetições (parcelas de 20 m2). O período de avaliação foi de um ano (01/12/2007 a 10/12/2008), com a realização de oito cortes, sendo três na primavera, três no verão, um no outono e um no inverno. A produção de massa seca (MS) da Xaraés foi maior no verão e na primavera em relação à Marandu. No somatório do ano a produtividade de MS da Xaraés foi 27,07% superior à Marandu, alcançando valor elevado (20.023 kg/ha), o que demonstra o potencial de utilização deste genótipo, principalmente em sistemas de produção de bovinos de corte mais intensivos. A produção de MS da Piatã nas estações do ano foi semelhante à Marandu, totalizando 17.572 e 15.757 kg/ha, respectivamente. Os dados de produção indicam que a Piatã apresentou a melhor distribuição da forragem produzida ao longo do ano. A produção de forragem das cultivares foi maior na altura do resíduo de 15 cm no verão e inverno, e não diferiu nas alturas de manejo no outono e primavera, demonstrando exigências diferentes de manejo do capim ao longo do ano. Os resultados obtidos confirmam a recomendação e a importância da cultivar Piatã como alternativa de utilização e diversificação das pastagens nos sistemas produtivos. / The experiment was conducted in the School of Veterinary Medicine and Animal Science of UNESP - Botucatu Campus. The experimental design was a complete randomized blocks in a factorial arrangement with three cultivars of Brachiaria brizantha (Marandu, Xaraés and BRS Piatã) and two stubble heights (15 and 25 cm), in the total six treatments with four replications (20 m² plots). The evaluation period was of one year (12/01/2007 to 12/10/2008) with eight cuts: three in the spring, three in the summer, one in the fall and one in the winter. The production of dry matter (DM) of Xaraés in summer and spring were higher than Marandu with a total of 27.07% higher DM than Marandu. The total DM production was 20,023 kg/ha, demonstrating the potential of use of the Xaraés cultivar, especially in more intensive systems of beef cattle production. DM production of Piatã in the seasons was similar to Marandu, totalizing 17,572 and 15,757 kg/ha, respectively. The production data indicate that Piatã cultivar had the best distribution of forage produced during the year. The forage production of cultivars was higher in the stubble height of 15 cm in summer and winter, and did not differ for two heights of management in the fall and spring, showing different requirements of grass management during the year. The results confirm the recommendation and the importance of Piatã cultivar as an alternative of use and diversification of pastures in the production systems.

Made available in DSpace on 2014-11-10T11:09:57Z (GMT). No. of bitstreams: 0 Previous issue date: 2014-02-28Bitstream added on 2014-11-10T11:57:31Z : No. of bitstreams: 1 000786133.pdf: 2473732 bytes, checksum: 2718404dbc454aa4d3963273c33b3ecc (MD5) / Introdução: Muitas patologias podem acometer a córnea, entre elas a síndrome do olho seco e as doenças relacionadas. Tratamentos inovadores para a síndrome do olho seco têm surgido, entre eles, o uso de colírio produzido com soro autólogo de uso ocular, soro de sangue de cordão umbilical, meio condicionado obtido pela expansão de células tronco do epitélio limbal (modelo ex vivo), entre outros. Pacientes com provável indicação do colírio de soro autólogo podem ter a contra-indicação na produção oriunda do status imune para doenças virais como HIV, hepatites B e C, sífilis, doença de Chagas e HTLV I e II, que do ponto de vista de produção do autocolírio, encontram barreiras. Para estes casos, o colírio de soro alogênico, oriundo de doadores saudáveis, é uma alternativa viável. Objetivos: Analisar o efeito in vitro da ação de colírio alogênico em cultura de células da região córneo-escleral, estabelecer a cultura e expansão de células aderentes obtidas da região córneo-escleral, comparar a cultura das células aderentes em meio de cultura contendo colírio e fatores de crescimento derivados de plaquetas, avaliar as células por meio da técnica de imunohistoquímica e analisar os dados médicos dos pacientes que utilizam autocolírio por meio de prontuários. Casuística e Métodos: Os anéis córneo-esclerais foram obtidos no centro cirúrgico após cirurgia de doação de córnea, sendo que estes anéis são materiais de descarte após a cirurgia. Em seguida, o tecido foi encaminhado ao Laboratório de Engenharia Celular do Hemocentro de Botucatu, onde foi fragmentado em pequenas partes e digerido com colagenase tipo I. As células foram plaqueadas em frascos de 25 cm² com meio DMEM Knockout, em uma primeira parte do experimento, e em meio Keratinocyte na segunda parte do trabalho. Após confluência de 80%, as células passaram pelo processo de tripsinização para desprendimento ... / Introduction: Many diseases can affect cornea, including dry eye syndrome and related diseases. Innovative treatments for dry eye syndrome have emerged , among them the use of autologous serum eye drops produced with the use of eye serum, umbilical cord blood, conditioned medium obtained by expansion of limbal epithelial stem cells (ex vivo model) among others. Patients with probable indication of autologous serum eyedrops may be contraindicated in production originating immune status for viral diseases such as HIV, hepatitis B and C, syphilis , Chagas disease and HTLV I and II, from the point of view of eye drops´s production, have barriers . For these cases, eye drops from healthy donors allogeneic serum can be an alternative. Objectives: Evaluate in vitro effect of the action of allogeneic eye drops in corneal-scleral cell culture, established the culture and expansion of adherent cells obtained from the corneal- scleral region, compare the culture of adherent cells in culture medium containing eye drops and platelets derived growth factors, evaluating the cells by immunohistochemistry and analyze medical data of eye drops´s patients. Materials and Methods: The corneal- scleral rings were obtained in the operating room after surgery of tissue donor, and these rings were discarded after surgery. Then, tissue was sent to the Laboratory of Cell Engineering of Botucatu Blood Center, which was fragmented into small pieces and digested with collagenase type I. Cells were plated in 25 cm² flasks with DMEM Knockout in the first part of the experiment, and Keratinocyte in the second part of the experiment. After 80% confluence, cells passed through the trypsinization procedure for detachment of the cells from the culture flask. Mounting plaque experiment, cells were placed to control, or untreated cells, treated cells with eye drops and cells treated with platelets derived growth factors was performed. After 5 days, immunohistochemistry ...

Doctor of Philosophy / Department of Animal Sciences and Industry / Christopher D. Reinhardt / Two studies evaluated dry-rolled corn (DRC) manufacturing considerations in feedlot finishing diets. In study 1, feedlots (n = 35) participated in a survey to evaluate dry-rolled corn (DRC) processing practices, processed corn particle size distribution, and fecal starch content in finishing cattle. Average particle size of dry-processed corn, including DRC and hammermill-ground corn across all operations (n = 35) was 4,223 ± 1,265 µm with a range of 1,165 to 6,823 µm. Fecal starch content averaged 19.0 ± 6.5% with a range of 7.0 to 36.6%. Diet composition was evaluated for co-product [27.8 ± 13.4%] roughage concentration [8.9 ± 2.0%] and NDF concentration [19.3 ± 4.3%]. In study 2, cross-bred yearling steers (n = 360; initial BW = 395 ± 33.1 kg) were used to evaluate the effects of dry-rolled corn (DRC) particle size in diets containing 20% (DMB) wet distiller’s grains plus solubles (WDGS) on feedlot performance, carcass characteristics, and starch digestibility. Treatments were Coarse DRC (4,882 µm; COARSE), Medium DRC (3,760 µm; MEDIUM), Fine DRC (2,359 µm; FINE), and Steam-flaked corn (SFC, 0.35 kg/L). Final BW and ADG were not affected by treatment (P > 0.05). Dry matter intake was greater and G:F was lower (P < 0.05) for steers fed DRC vs. SFC. There was a linear decrease (P < 0.05) in DMI in the final 5 weeks on feed with decreasing DRC particle size. Fecal starch decreased (linear, P < 0.01) as DRC particle size decreased. In situ starch disappearance was lower for DRC vs SFC (P < 0.05) and increased linearly (P < 0.05) with decreasing particle size at 8 and 24-h. The final study evaluated steam-flaked corn (SFC) manufacturing practices implemented, equipment utilized, and methods used and parameters targeted to measure flake quality from commercial feedlots (n = 17). Significant variables contributing to the final multiple linear regression model using enzymatic starch availability (Enzymatic) as the dependent variable were: SFC Moisture, cooled flake density (CoolFD), throughput, roll diameter, steam cabinet temperature (Temperature), and temper time (Enzymatic = 19.4476 - (0.6927*SFCMoisture) - (2.1664*CoolFD) - (0.5060*Throughput) + (0.6281*Roll Diameter) + (0.4312*Temperature) – (0.1963*Temper Time; P < 0.15).

Doctor of Philosophy / Department of Entomology / Thomas W. Phillips / The phase-out of methyl bromide production, the most effective fumigant for the control of the ham mite, Tyrophagus putrescentiae (Schrank) (Acari: Acaridae), on dry-cured ham has necessitated the search for other management methods. The foundation of a successful management program is an effective monitoring program that provides information on pest presence and abundance over time and space to help in making management decisions. By using the standard trap made from disposable Petri dishes and a dog food-based bait, mite activity was monitored weekly in five dry-cured ham aging rooms from three commercial processing facilities from June 2012 to September 2013. Results indicated that mite numbers in traps in facilities typically had a pattern of sharp decline after fumigation, followed by a steady increase until the next fumigation. Average trap captures varied due to trap location, indicating that traps could be used to identify locations where mite infestation of hams may be more likely to occur. Experiments were also conducted in 6 m x 3 m climate-controlled rooms to determine the effects of some physical factors on trap capture. Factors such as trap design, trap location, trap distance, duration of trapping, and light conditions had significant effects on mite capture. Mites also responded differently to light emitting diodes of different wavelengths, either as a component of the standard trap or as a stand-alone stimulus to orientation. To determine the relationship between trap capture and mite density, experiments were carried out in the climate-controlled rooms. Mite density was varied but trap number remained constant for all mite densities. There was strong positive correlation between trap capture and mite density. In simulated ham aging rooms, the distribution of mites on hams was determined and different sampling techniques such as vacuum sampling, trapping, rack sampling, ham sampling and absolute mite counts from whole hams were compared and correlated. Results showed weak or moderate correlations between sampling techniques in pairwise comparisons. Two sampling plans were developed to determine the number of samples required to estimate mite density on ham with respect to fixed precision levels or to an action threshold for making pest management decisions. Findings reported here can help in the optimization of trapping and sampling of ham mite populations to help in the development of efficient, cost-effective tools for pest management decisions incorporated with alternatives to methyl bromide.

Master of Science / Department of Biological & Agricultural Engineering / Donghai Wang / Camelina sativa (L). Crantz that belongs to Brassicaceae family has been grown as a dicotyledonous oilseed crop in the cold places like America and Canada. Camelina seeds are widely used for the extraction of oil and protein. Recently, research found that camelina gum is an excellent candidate for food and industrial uses as thickener or stabilizer. The objectives of this research were 1) to increase camelina gum isolation efficiency using spray drying technology, and 2) to develop an innovative method to remove gum from seed bran to increase protein and oil extraction efficiency and quality. The camelina gums isolated using ethanol precipitation and spray drying method from the whole camelina seeds were compared. Effects of spray drying temperature on yield, gum morphology, and gum rheological and thermal properties were studied. The representative sample dried at 165°C was chosen to study the effects of concentration, temperature, pH and additives (NaCl, CaCl₂, sucrose, and ethanol) on viscosity and viscoelastic properties of the isolated gum. The gum showed a shear thinning behavior when shear rate increased gradually, higher concentrations of additives only slightly affect the rheological properties. Results showed that spray drying is an effective method in terms of saving time and energy, and provided positive rheology benefits on camelina gum isolation. Pre-removal of gum from camelina seeds can increase protein and oil yield and their quality. Decortication can separate 10-17% of the total camelina seed as bran. A wind tunnel was used to separate lighter bran particles from heavier endosperm and unbroken seeds. Camelina gum isolation from the separated seed bran using the traditional ethanol precipitation method was optimized using response surface methodology where the simultaneous effect of the three independent variables (seed bran to water ratio, isolation temperature, and isolation time) were investigated for gum yield, purity, and optimum rheological properties. Three independent quadratic modules were developed and the original data fitted the models fitted (R² = 0.995, 0.877, and 0.804). The optimal isolation conditions were seed bran to water ratio of 1:39, isolation temperature of 35 ºC, and isolation time of 1.5 h and 0.839 desirability was obtained by the rigorous statistics analysis. The protein yield and quality extracted from decorticated endosperm were improved significantly compared with that extracted from whole seeds meal without decortication. In addition, the degumming step can be eliminated before protein and oil extraction that increase protein and oil extraction efficiency.

abstract: Bioretention basins are a common stormwater best management practice (BMP) used to mitigate the hydrologic consequences of urbanization. Dry wells, also known as vadose-zone wells, have been used extensively in bioretention basins in Maricopa County, Arizona to decrease total drain time and recharge groundwater. A mixed integer nonlinear programming (MINLP) model has been developed for the minimum cost design of bioretention basins with dry wells. The model developed simultaneously determines the peak stormwater inflow from watershed parameters and optimizes the size of the basin and the number and depth of dry wells based on infiltration, evapotranspiration (ET), and dry well characteristics and cost inputs. The modified rational method is used for the design storm hydrograph, and the Green-Ampt method is used for infiltration. ET rates are calculated using the Penman Monteith method or the Hargreaves-Samani method. The dry well flow rate is determined using an equation developed for reverse auger-hole flow. The first phase of development of the model is to expand a nonlinear programming (NLP) for the optimal design of infiltration basins for use with bioretention basins. Next a single dry well is added to the NLP bioretention basin optimization model. Finally the number of dry wells in the basin is modeled as an integer variable creating a MINLP problem. The NLP models and MINLP model are solved using the General Algebraic Modeling System (GAMS). Two example applications demonstrate the efficiency and practicality of the model. / Dissertation/Thesis / Masters Thesis Civil Engineering 2016

abstract: It is well known that the geckos can cling to almost any surface using highly dense micro/nano fibrils found on the feet that rely on Van Der Waals forces to adhere. A few experimental and theoretical approaches have been taken to understand the adhesion mechanism of gecko feet. This work explains the building procedure of custom experimental setup to test the adhesion force over a temperature range and extends its application in space environment, potentially unsafe working condition. This study demonstrates that these adhesive capable of switching adhesive properties not only at room environment but also over a temperature range of -160 degC to 120 degC in vacuum conditions. These conditions are similar to the condition experienced by a satellite in a space orbiting around the earth. Also, this study demonstrated various detachment and specimen patch preparation methods. The custom-made experimental setup for adhesion test can measure adhesion force in temperature and pressure controlled environment over specimen size of 1 sq. inch. A cryogenic cooling system with liquid nitrogen is used to achieve -160 degC and an electric resistive heating system are used to achieve 120 degC in controlled volume. Thermal electrodes, infrared thermopile detectors are used to record temperature at sample and pressure indicator to record vacuum condition in controlled volume. Reversibility of the switching behaviour of the specimen in controlled environment confirms its application in space and very high or very low-temperature conditions. The experimental setup was developed using SolidWorks as a design tool, Ansys as simulation tool and the data acquisition utilizes LabVIEW available in the market today. / Dissertation/Thesis / Masters Thesis Mechanical Engineering 2016

High-performance fibres such as carbon, glass and kevlar are very promising for aerospace applications because of their high strength, stiffness, impact damage and excellent fatigue life. The high cost of the prepreg materials such as pre-impregnated fibre tape/tow and fabrics, and limitations of existing manufacturing processes are a big challenge for the aerospace industry to meet increasing performance demands. Their benefits can only be achieved by using low cost materials and manufacturing methods. In the past three or four decades, there have been substantial technological developments, which are governed by the new materials and their associated manufacturing techniques. The production of carbon fibre is slow and capital intensive, therefore, carbon manufactures produce higher tow counts (number of filaments) to increase production through-put in order to reduce its cost. In other words, 12k carbon tow is much cheaper than 6k or 3k carbon tow. In many applications finer tows are desirable. In this thesis, a fully automated laser feedback tow splitting line has been developed to split higher tow counts (12k spool) into smaller tow counts (split into 6k spools) in order to produce low cost material. The quality of the split tows has been evaluated by recording the data online during the splitting process. The recorded data was later analysed by statistical tools. A four axis modular gantry robotic system has been developed at the University of Manchester in order to deposit dry fibres in a completely flexible manner. To facilitate robotic preforming, an end-effector and mould have been designed and developed in this research. The tow placement program was written in the CoDeSys software which is then uploaded into the motion controller to perform specific motions. The cross-ply laminates have been manufactured by the proposed robotic system using split 6k (produced by the tow splitting process) and original 12k carbon tows. Mechanical test of both composites (12k and split 6k) are presented. A tufting process has been developed and conducted by the robotic system in order to manufacture 3D preforms. The tufted composite was compared with 3D woven and stitched 2D broadcloth in terms of the tensile and interlaminar shear strength properties. X-ray tomography has been conducted to investigate preform geometrical variations of manufactured composites. In addition, preforming cost models have been developed for robotic fibre placement and 3D weaving.

Orientador: Luiz Eduardo de Ângelo Sanchez / Banca: Vicente Luiz Scalon / Banca: Alisson Rocha Machado / Resumo: A crescente produtividade de aumento na produtividade em operações de usinagem toma cada vez mais importante o desenvolvimento de novas ferramentas de corte e novos métodos de manufatura, os quais devem ter a capacidade de preencher a demanda atual. Deste modo, muitos esforços têm sido direcionados para permitir a utilização de velocidade de corte cada vez maiores. Um grande desafio é controlar a temperatura durante o processo de usinagem, uma vez que a temperatura aumenta com o aumento da velocidade de corte, reduzindo a dureza a quente da ferramenta e alimentando os mecanismos de desgaste. Para minimizar estes efeitos, vários métodos de refrigeração têm sido propostos, cada um com suas vantagens e desvantagens. Os métodos convencionais de refrigeração, que utilizam fluidos de corte, embora possuam eficiência reconhecida, adicionam custos ao processo, além de serem causadores de problemas relacionados com o meio ambiente e com a saúde dos operadores. Neste contexto a usinagem a seco, associada com o emprego de ferramenta com alta dureza a quente, tem sido um bom método para evitar os problemas mencionados. Outra opção é a usinagem criogênica, que utiliza ferramentas de metal duro em temperaturas abaixo de -150ºC, utilizando, para isso, nitrogênio líquido como fluido refrigerante. Entretanto, este método traz alguns problemas, como a necessidade de equipamentos especiais com tamanho significante ao lado da máquina-ferramenta. Neste estudo é proposto o desenvolvimento e a construção de um sistema de refrigeraçã de ferramenta para o processo de tornemaneto, com baixo custo e manutenção simples, composto por um porta-ferramenta, com um fluido refrigerante passando internamente ao seu corpo em um circuito fechado, onde o fluido evapora em uma câmara abaixo do inserto de usinagem, removendo assim calor da ferramenta. O fluido refrigerante passa então através... (Resumo completo, clicar acesso eletrônico abaixo) / Abstract: The growing need of increase in productivity in machining operations emphasizes the importance of the development of new cutting tools and new manufacturing methods, which have the capacity to fulfill the present demand. In this way, many efforts are directed to enable the utilization of higher cutting speeds. One great challenge is to control the temperature during the machining process, since the temperature rises with the increase of the cutting speed, reducing the hot hardness of the cutting tool and accelerating the tool wear mechanism. To minimize these effects, many cooling methods have been proposed, each one with advantages and disadvantages. The conventional cooling methods, which use cutting fluids, although have recognized efficiency, add costs to the process, besides to cause problems regarding to the environment and operators health. In this context, dry machining, associated with the employment of tools with high hot hardness, has been a good method to avoid these problems. Another option is the cryogenic machinig, which utilizes carbide tools in temperatures lower than - 150ºC, using, for this, liquid nitrogen as cooling fluid. However, this method brings some problems, like the need of special devices with significant size around the machine-tool. In this work, it is proposed the development and the construction of a cooling tool system for turning process, with low cost and simple maintenance, composed by a tool-holder, with a cooling fluid flowing within its body in a loop circuit, where the fluid evaporates just under the insert location, removing heat from it. The cooling fluid passes through a heat exchanger where it condensates and a new cyble is started. As result the development system provides a tool life equal or better than with the cutting fluid application, with clear economic and environmental advantages / Mestre