The Lake Clearwater catchment, in the Canterbury high country of New Zealand, has a native ecosystem that is adapted to low nutrient conditions. Wetlands in the catchment are identified by the Department of Conservation’s Arawai Kākāriki Wetland Restoration Programme as one of three important endemic wetland types in New Zealand. Uncertainty regarding diffuse nutrient load from agriculture into the lake and wetland ecosystems is limiting effective management of the catchment. This study investigated hydrological processes and nitrogen and phosphorus concentrations to improve knowledge of the sources, characteristics and magnitude of nutrient loading from agricultural land use in this 46 km2 high country catchment. Relevant hydrological data and literature pertaining to the catchment was extensively reviewed. In addition, flow for five key surface waterways was continuously logged at ten sites for 2 years. Concurrently, nutrient concentrations for total nitrogen, nitrate, ammoniacal nitrogen, total phosphorus and dissolved reactive phosphorus were measured at ten surface water sites and three groundwater sites. Total nitrogen and phosphorus load from farmland was calculated from annual flow and median concentrations for four waterways: farmland perennial stream runoff, farmland ephemeral stream runoff, a wetland channel below the farmed hillslope and the lake outlet. Similarly, total nitrogen and phosphorus load for unfarmed land was calculated from the flow and median concentration of two un-impacted perennial streams. Total nitrogen and phosphorus mass balances were calculated and used to estimate subsurface nutrient load and runoff volume from the farmed hillslope. Estimates of subsurface runoff were also made using Darcy’s equation and a water balance. Nutrient load predictions from the Catchment Land Use for Environmental Sustainability (CLUES) model were compared to measured loads. Nutrients were found to be elevated downstream of farmland, especially nitrogen, which was often above relevant guidelines and typical concentrations in upland waterways in Canterbury. Nitrate in farmland subsurface runoff was elevated and was estimated to contribute 52% of total nitrogen yield from farmland. Total nitrogen yield (1.96-2.94 kg ha-1 year-1) for farmed land was comparable to minimum values for pastoral land use in literature but total phosphorus yield (0.093-0.123 kg ha-1 year-1) was well below published values. The range in yield estimates is due to subtraction of a high and a low estimate of natural baseline yield from the measured in-stream yield. Total nitrogen export from the lake (2518 kg year-1) was greater than estimated input (1375 kg year-1) from farmed and non-farmed land indicating an additional source of nitrogen into Lake Clearwater. Total phosphorus export from Lake Clearwater of 58 kg year-1 was 24% less than total estimated loads into the lake (76 kg year-1) from farmed and non-farmed land. Phosphorus was not often above relevant guidelines and the median total nitrogen to total phosphorus ratio in Lake Clearwater (49:1) indicated phosphorus is the limiting nutrient in the lake. Because phosphorus was less elevated relative to nitrogen, an increase in phosphorus inputs could have a greater effect on productivity in the wetland and lake. With corrected land use information, total nitrogen loads predicted by the CLUES model were reasonable but total phosphorus loads were greatly overestimated. Investigation into potential impacts of the elevated nutrient loads described in this study on receiving native ecosystems is recommended to inform conservation efforts.

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This thesis investigates the application of ultra-high modulus carbon fiber reinforced polymer (CFRP) plates to strengthen damaged reinforced concrete beams and slender columns. In the first phase, two different pre-repair loading histories were simulated in seven 3000x300x150 mm reinforced concrete beams, namely cracking within the elastic range, and overloading in the plastic range. After unloading, the beams were repaired with either high- or ultra-high modulus (210 or 400 GPa) CFRP plates, or a hybrid system, and then reloaded to failure. It was shown that the level of pre-existing damage has an insignificant effect on the strengthening effectiveness and the failure mode at ultimate. The 210 and 400 GPa CFRP of reinforcement ratio ρf = 0.17% increased the ultimate strength by up to 29 and 51%, respectively, despite the 40% lower tensile strength of the 400 GPa CFRP, due to the change in failure mode from debonding to rupture. Doubling ρf of the 400 GPa CFRP to 0.34% resulted in a 63% overall gain in flexural strength, only 8% increase in ultimate strength over ρf = 0.17%, due to change in failure mode from rupture to concrete cover delamination. The beam retrofitted by hybrid CFRP showed remarkable pseudo ductility and warning signs before failure. However, a parametric study revealed a critical balance in proportioning the areas of hybrid CFRP to achieve reliable pseudo ductility. In the beam with ρf =0.34%, this was achieved using a maximum of 30% ρf of the 400 GPa CFRP. The second phase of this thesis presents an analytical model developed by modifying the provisions of the ACI 318-08 code and employing the computer software Response 2000, to predict the performance of CFRP strengthened slender reinforced concrete columns. Response 2000 is used to establish the interaction curve while the modified ACI 318-08 code is used to acquire the slender column loading path to failure including the second order effects. The model predicts that the effectiveness of the FRP strengthening system increases as the slenderness ratio and FRP reinforcement ratio increase. / Thesis (Master, Civil Engineering) -- Queen's University, 2013-09-24 12:36:48.352

Reinforced Concrete (RC) bridge piers, RC columns along exterior of buildings or those located in parking garages are designed to support large compressive axial loads but are vulnerable to transverse out-of-plane loadings, such as those arising from impacts or explosions. To address a lack of understanding regarding blast and impact response of RC members and the need for retrofit techniques to address deficiencies in existing structures, a multi-disciplinary team including various institutes of the National Research Council and the University of Ottawa has initiated work towards developing a fibre reinforced polymer composite protection system for RC columns subjected to extreme shocks. This thesis will focus on the impact program of the aforementioned project. An extensive literature review was conducted to gain a better understanding of: impact loading and associated dynamic effects; experimental testing of RC members subjected to impact; experimental testing of axially loaded members; and retrofit methods for the protection of RC under impact loading. Five half-scale RC columns were constructed and tested using a drop-weight impact machine and two additional specimens were tested under static loading. Deflections, strain distributions within the columns, impact loads and reaction loads were measured during the testing of the built RC members. Comparisons of experimental datum were established between members with differing levels of axial load and between a retrofitted and a non-retrofitted member. Single-degree-of-freedom analysis was used to obtain the predicted response of certain columns under impact loading allowing for comparisons with experimental data.

In vitro studies have shown that the major membrane phospholipid phosphatidylcholine (PC) can positively influence the incorporation of cholesterol in lipid membranes. The influence of PC on the cellular trafficking of LDL-derived free cholesterol was investigated. Sterol regulatory-defective (SRD)-4 cells are Chinese hamster ovary (CHO)-derived fibroblasts that display vastly elevated rates for the synthesis and catabolism of PC. SRD-4 cells harbor two known gene mutations: a mutation in the functional allele for SCAP, resulting in defective feedback suppression of cholesterol biosynthesis; and a loss-of-function mutation in the functional allele for acyl-CoA:cholesterol acyl transferase (ACAT), an endoplasmic reticulum (ER)-localized enzyme that esterifies free cholesterol. Incubation of SRD-4 cells with 50 µg/ml low density lipoprotein (LDL) for 18 h resulted in lysosomal accumulation of free cholesterol as revealed by filipin staining. This accumulation was not evident following LDL treatment of parental CHO7 cells, and was blunted in SRD-2 cells that express a constitutively-active form of SREBP-2 and overproduce cholesterol but have functional ACAT activity. Treatment of SRD-2 cells with LDL in the presence of an ACAT inhibitor 58-035 resulted in robust lysosomal cholesterol accumulation that was reversible upon drug washout, supporting that cholesterol trafficking in cholesterol-loaded cells is dependent on ACAT activity and, more specifically, ER free cholesterol levels. Lysosomal accumulation of LDL-derived cholesterol was prevented in SRD-4 cells supplemented with lyso-PC (50 µM), a substrate for PC synthesis through the reacylation pathway, and also in cells treated with bromoenol lactone (BEL), an inhibitor of phospholipase A2 implicated in bulk PC turnover. In a counter study, lysosomal LDL-derived cholesterol accumulation was induced in parental CHO-7 cells using R-propranolol, which inhibits the conversion of phosphatidic acid to diacylglycerol (DAG), a substrate in the CDP-choline pathway. This blockage was also relieved through co-treatment with lyso-PC. These studies support that PC to free cholesterol ratios in downstream organellar membranes can influence cholesterol trafficking out of lysosomal compartments in cholesterol-loaded cells.

Hedging a contingent claim with an asset which is not perfectly correlated with the underlying asset results in an imperfect hedge. The residual risk from hedging with a correlated asset is priced using an actuarial standard deviation principle in infinitesmal time, which gives rise to a nonlinear partial differential equation (PDE). A fully implicit, monotone discretization method is developed for solving the pricing PDE. This method is shown to converge to the viscosity solution. Certain grid conditions are required to guarantee monotonicity. An algorithm is derived which, given an initial grid, inserts a finite number of nodes in the grid to ensure that the monotonicity condition is satisfied. At each timestep, the nonlinear discretized algebraic equations are solved using an iterative algorithm, which is shown to be globally convergent. Monte Carlo hedging examples are given, which show the standard deviation of the profit and loss at the expiry of the option.

The topic of structural damage caused by fires following an earthquake (FFE) has been discussed extensively by many researchers for over a decade in order to bring the two fields closer together in the context of performance based structural engineering. Edinburgh University, Heriot-Watt University, Indian Institute of Technology Roorkee (IIT Roorkee) and Indian Institute of Science initiated a collaboration to study this problem under a UK-India Engineering Research Initiative (UKIERI) funded project. The first construction of a single-storey reinforced concrete frame at IIT Roorkee was completed in summer 2011; this is known as the Roorkee Frame Test 1 throughout this thesis. This thesis presents the modelling of the Roorkee Frame Test 1 using the finite element method and assesses the capability of the numerical methodologies for analysing these two sequential events. Both two and three dimensional finite element models were developed. Beam and shell elements were chosen for the numerical modelling, which was carried out using the general purpose finite element package ABAQUS (version 6.8). The variation in material properties caused by these two types of loading, including strength and stiffness degradation, compressive hardening, tension stiffening, and thermal properties, is implemented in the numerical modelling. Constitutive material calculations are in accordance with EC4 Part 1.1, and all loading is according to IS 1893:2002 Part 1 (Indian Standard). The time-temperature curve used in the analysis is based on data from the test carried out. The behaviour of the Roorkee Frame Test 1 when subjected to monotonic, cyclic lateral loading followed by fire is presented. The capacity of the frame when subjected to lateral loading is examined using a static non-linear pushover method. Incremental lateral loading is applied in a displacement-controlled manner to induce simulated seismic damage in the frame. The capacity curve, hysteresis loops and residual displacements are presented, discussed and compared with the test results. The heat transfer analysis using three dimensional solid elements was also compared against temperature distributions recorded during the Roorkee frame fire test. Based on the smoke layer theory, two emissivity values were defined. In this study, the suitability of numerical modelling using ABAQUS to capture the behaviour of Roorkee frame test is examined. The results from this study show that the 3D ABAQUS model predicted more reliable hysteresis curves compared to the 2D ABAQUS model, but both models estimated the lateral load capacity well. However neither model was able to simulate the pinching effect clearly visible in the hysteresis curves from the test. This was due to noninclusion of the bond slip effect between reinforcing bars and concrete. The residual displacement obtained at the end of the cyclic lateral loading analysis from the 2D ABAQUS model is higher than that seen in the test. However, the result in the 3D ABAQUS model matched the trend obtained in the test. The both columns appear to stiffen under the heating and the residual displacement seems to recover slightly. Lateral displacements, obtained in the thermo-mechanical analysis of the numerical models, show that thermal expansion brings the frame back towards its initial position. Finally, correlation studies between analytical and experimental results are conducted with the objective to establish the validity of the proposed model and identify the significance of various effects on the local and global response of fire resistance earthquake damaged of reinforced concrete frames. These studies show that the effect of tension stiffening and bond-slip are very important and should always be included in finite element models of the response of reinforced concrete frame with the smeared crack model when subjected to lateral and thermal loading. The behaviour of reinforced concrete frames exposed to fire is usually described in terms of the concept of the fire resistance which defined in terms of displacement limit. This study shows the global displacement of the frame subjected to fire recover slightly due to the thermal expansion during the heating.

<p>The successful, efficient, and safe turbine design requires a thorough understanding of the underlying physical phenomena. This research investigates the physical understanding and parameters highly correlated to flutter, an aeroelastic instability prevalent among low pressure turbine (LPT) blades in both aircraft engines and power turbines. The modern way of determining whether a certain cascade of LPT blades is susceptible to flutter is through time-expensive computational fluid dynamics (CFD) codes. These codes converge to solution satisfying the Eulerian conservation equations subject to the boundary conditions of a nodal domain consisting fluid and solid wall particles. Most detailed CFD codes are accompanied by cryptic turbulence models, meticulous grid constructions, and elegant boundary condition enforcements all with one goal in mind: determine the sign (and therefore stability) of the aerodynamic damping. The main question being asked by the aeroelastician, ``is it positive or negative?'' This type of thought-process eventually gives rise to a black-box effect, leaving physical understanding behind. Therefore, the first part of this research aims to understand and reveal the physics behind LPT flutter in addition to several related topics including acoustic resonance effects. A percentage of this initial numerical investigation is completed using an influence coefficient approach to study the variation the work-per-cycle contributions of neighboring cascade blades to a reference airfoil. The second part of this research introduces new discoveries regarding the relationship between steady aerodynamic loading and negative aerodynamic damping. Using validated CFD codes as computational wind tunnels, a multitude of low-pressure turbine flutter parameters, such as reduced frequency, mode shape, and interblade phase angle, will be scrutinized across various airfoil geometries and steady operating conditions to reach new design guidelines regarding the influence of steady aerodynamic loading and LPT flutter. Many pressing topics influencing LPT flutter including shocks, their nonlinearity, and three-dimensionality are also addressed along the way. The work is concluded by introducing a useful preliminary design tool that can estimate within seconds the entire aerodynamic damping versus nodal diameter curve for a given three-dimensional cascade.</p> / Dissertation

The container loading problem considered in this thesis is to determine placements of a set of packages within one or multiple shipping containers. Smaller packages are consolidated on pallets prior to being loaded in the shipping containers together with larger packages. There are multiple objectives which may be summarized as fitting all the packages while achieving good stability of the cargo as well as the shipping containers themselves. According to recent literature reviews, previous research in the field have to large extent been neglecting issues relevant in practice. Our real-world application was developed for the industrial company Atlas Copco to be used for sea container shipments at their Distribution Center (DC) in Texas, USA. Hence all applicable practical constraints faced by the DC operators had to be treated properly. A high variety in sizes, weights and other attributes such as stackability among packages added complexity to an already challenging combinatorial problem. Inspired by how the DC operators plan and perform loading manually, the batch concept was developed, which refers to grouping of boxes based on their characteristics and solving subproblems in terms of partial load plans. In each batch, an extensive placement heuristic and a load plan evaluation run iteratively, guided by a Genetic Algorithm (GA). In the placement heuristic, potential placements are evaluated using a scoring function considering aspects of the current situation, such as space utilization, horizontal support and heavier boxes closer to the floor. The scoring function is weighted by coefficients corresponding to the chromosomes of an individual in the GA population. Consequently, the fitness value of an individual in the GA population is the rating of a load plan. The loading optimization software has been tested and successfully implemented at the DC in Texas. The software has been proven capable of generating satisfactory load plans within acceptable computation times, which has resulted in reduced uncertainty and labor usage in the loading process. Analysis using real sea container shipments shows that the GA is able to tune the scoring coefficients to suit the particular problem instance being solved.

A systematic investigation is presented into the microstructural and micromechanical influences on ductile damage nucleation with an emphasis on grain boundaries in polycrystals. Microstructures obtained from experiments on copper polycrystals are characterized using Electron Backscatter Diffraction (EBSD) and near-field High-Energy Diffraction Microscopy (nf-HEDM) and the occurrence of damage is compared with micromechanical values obtained using an elasto-viscoplastic model based on the Fast- Fourier Transform (EVPFFT). The model produces full-field solutions for the stress and strain in voxelized polycrystalline microstructures. In order to resolve the fields onto interfaces, local Cartesian moments of the polycrystalline grain structure are used to extract the normals of grain boundaries and the tangents of triple junctions directly from the voxelized microstructure. Thus projecting the stress yields a parameter with potential significance, i.e. the grain boundary surface tractions. We identify “traction hotspots”, i.e. regions with tractions that are significantly above the mean, for the case of uniaxial tension. These show correlations with the angle between the grain boundary normal and the loading axis, a trend that some experiments also show when boundaries that nucleated voids are analyzed using EBSD, though differences present between the simulation and experiment hint that further criteria are needed. Nf-HEDM was used to record microstructure images of a polycrystalline sample before and after it undergoes damage. The damage locations in the post-shocked image are mapped onto the pre-shocked image, allowing stress and strain values from the EVPFFT model in the regions that eventually nucleated damage to be correlated with the locations of the void. The unexpected result was that differences in plastic work across boundaries correlated with voids, whereas vi quantities such as triaxiality and normal forces across boundaries did not.