Thesis (M.S.)--Virginia Polytechnic Institute and State University, 1993. / Vita. Abstract. Includes bibliographical references (leaves 70-72). Also available via the Internet.

Thesis (Ph. D.)--University of Washington, 2002. / Vita. Includes bibliographical references (leaves 239-243).

A recently developed optical method utilizes a single, implantable, optical fiber to record electrical activity from isolated hearts stained with voltage-sensitive dyes. This optical technique generates recordings of transmembrane potential from excitable myocardial tissue, and remain free from stimulus artifacts that accompany electro stimulation and hinder all standard electrode recording methods during the application of high-voltage electrical shocks. The fiber optic system uses a l00J,lm diameter core fiber which can record from epicardial surface, internal mid-myocardium, or endocardial surfaces. The stained tissue is excited through the fiber and the resulting fluorescence is transmitted through the same fiber to a photomultiplier tube. Changes in fluorescence accompanying normal cardiac action potentials usually range from 1-5%. Substantive motion related signals accompany normal beating hearts, drowning out the actual signal that corresponds to change in membrane potential. When added to the nutrient solution of the heart, an excitation / contraction decoupler restricts motion and reduces the motion related signal making it easier to isolate the true membrane potential signal. / Master of Science

This work investigated the application of fiber-based endoscopes (FBEs) in combustion and flow measurements, especially for multidimensional and quantitative measurements. The use of FBEs offers several unique advantages to greatly reduce the implementation difficulty and cost of optical diagnostics. However, the use of FBEs requires registering the locations and orientations of the FBEs carefully for quantitative measurements, and degrades the spatial resolution of the images transmitted. Hence this work conducted a series of controlled tests to quantify the accuracy of the view registration process and the spatial resolution degradation for FBEs. The results show that, under the conditions tested in this work, the view registration process can be accurate within ±0.5 degree and the FBEs can resolve spatial features on the order of 0.25 mm. The combined effects of such view registration uncertainty and spatial resolution degradation are reflected in the re-projection error, which was shown to be within ±0.5 pixels under typical conditions used in this work. Finally, based on these understanding, experiments were conducted to obtain instantaneous measurements of flame structures at kHz temporal resolution using FBEs, demonstrating the capability of resolving flame features on the order of 0.2~0.3 mm in three-dimensional. / Master of Science

by Chan Yawen. / Thesis submitted in: September 12, 2005. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2005. / Includes bibliographical references (leaves 57-64). / Abstracts in English and Chinese. / Acknowledgement --- p.ii / Abstract --- p.iv / 摘要 --- p.vii / Table of Contents --- p.ix / List of Figures --- p.xii / List of Tables --- p.xiv / Chapter Chapter 1 --- Introduction --- p.1 / Chapter 1.1 --- Diseases of the Gastrointestinal (GI) Tract --- p.1 / Chapter 1.2 --- Wireless Capsule Endoscopy --- p.2 / Chapter 1.3 --- Goals of My Research Project --- p.9 / Chapter Part I - --- Experimental Study to Determine the Frequency of Wireless Transmission --- p.11 / Chapter Chapter 2 --- Background --- p.11 / Chapter 2.1 --- Analog and Digital Wireless Video Transmission --- p.11 / Chapter 2.2 --- "Industrial, Scientific and Medical (ISM) Bands" --- p.11 / Chapter 2.3 --- Adsorption of RP Energy by Biological Tissue --- p.13 / Chapter 2.4 --- Frequency used by Implanted/Ingested Devices --- p.13 / Chapter 2.5 --- Incentives of using Higher Frequencies --- p.14 / Chapter 2.6 --- Radiation Efficiency from an Implanted/Ingested Source --- p.15 / Chapter Chapter 3 --- Material and Method --- p.18 / Chapter 3.1 --- Human Body Trunk Experimental Model --- p.18 / Chapter 3.2 --- Radiating and Receiving Antennas --- p.19 / Chapter 3.3 --- Experimental Procedures --- p.21 / Chapter Chapter 4 --- Results and Discussions --- p.23 / Chapter Chapter 5 --- Conclusions --- p.30 / Chapter Part II - --- Prototype Design and Implementation --- p.31 / Chapter Chapter 6 --- Background --- p.31 / Chapter 6.1 --- Prototype Overview --- p.31 / Chapter 6.2 --- Digital and Analog Cameras --- p.32 / Chapter 6.3 --- Digital and Analog Transmitters --- p.34 / Chapter Chapter 7 --- Possible Solutions --- p.38 / Chapter 7.1 --- Analog Camera + Analog Video Transmission --- p.38 / Chapter 7.2 --- Digital Camera + Analog Video Transmission --- p.38 / Chapter 7.3 --- Digital Camera + Digital Video Transmission using WLAN Technology --- p.40 / Chapter 7.4 --- Digital Camera + Digital Video Transmission with Video Compression --- p.42 / Chapter Chapter 8 --- Implementation of the Analog Camera + Analog Transmission Solution --- p.44 / Chapter 8.1 --- Circuit Implementation --- p.44 / Chapter 8.2 --- System Verification --- p.49 / Chapter 8.3 --- Conclusions --- p.51 / Chapter Chapter 9 --- Conclusions and Future Work --- p.53 / Chapter 9.1 --- General Conclusions --- p.53 / Chapter 9.2 --- Future Work --- p.55 / List of Abbreviations --- p.65

As dificuldades do reprocessamento dos endoscópicos gastrointestinais, o risco de contaminação e os casos de infecções representam desafios amplamente conhecidos na comunidade cientifica. Neste sentido, investigou-se o reprocessamento de endoscópios gastrointestinais subsidiado nos níveis de sujidade, contaminação microbiana e presença de biofilme. Trata-se de um experimento laboratorial realizado em três fases por meio da bioluminescência com adenosina trifosfato (ATP) para avaliação do nível de sujidade; polimerase chain reaction (PCR) para carga bacteriana e cultura microbiana para determinação do nível de contaminação. Ainda, avaliou-se as superfícies internas de canais de endoscópios por microscopia eletrônica de varredura (MEV) quanto à presença de biofilme. A análise estatística dos dados foi subsidiada em medidas de tendência central, testes de Man-Whitney, Wilcoxon e Spearman, p<0,05, por meio do software IBM SPSS Statistics versão 23.0. A avaliação de 99 endoscópios antes e após limpeza manual demonstrou a eficácia do processo na redução de sujidade (p<0,001) e contaminação microbiana (p=0,03), inclusive com baixo percentual da amostra com micro-organismos viáveis. Dos 75 endoscópios avaliados após o reprocessamento evidenciou-se uma redução do nível de sujidade (todos com <50URL, interna e externamente); entretanto a presença de carga bacteriana foi de 3log de bactéria/mL e 10,6% de positividade das culturas. Os micro-organismos isolados dos lavados de endoscópios após o reprocessamento foram Staphylococcus aureus, Staphylococcus epidermidis, Staphylococcus hominis, Staphylococcus capitis, Roseomonas gilardii e Micrococcus luteus. Na avaliação dos canais de biópsia de endoscópios provenientes do Brasil observou-se maior contaminação do que os da Austrália (p<0,001). Todos os canais apresentaram biofilme e danos em suas superfícies, entretanto as amostras brasileiras apresentaram particularidades, como, presença de hemácias, neutrófilos e fungos. Assim, observou-se que apesar da baixa carga microbiana nos endoscópios há o risco potencial de infecção cruzada associado ao biofilme, ameaçando a qualidade e segurança do reprocessamento. Adiciona-se a necessidade de avanços tecnológicos e científicos contra o biofilme na prática do reprocessamento de endoscópios / The difficulties on gastrointestinal endoscope reprocessing, contamination risk and outbreaks are a recognized challenge in science. It was investigated gastrointestinal endoscope reprocessing for dirtiness, contamination level and presence of biofilm. Laboratorial experiment performed in three phases using adenosine triphosphate (ATP) bioluminescence for dirtiness evaluation; polymerase chain reaction (PCR) for bacterial load and microbial culture for contamination level evaluation. In addition, it was evaluated biofilm on endoscope channels internal surfaces by scanning electron microscopy (SEM). Statistics analysis was performed by descriptive analysis, ManWhitney, Wilcoxon and Spearman tests, p<0,05, using IBM SPSS Statistics versão 23.0. Before and after cleaning analysis of 99 endoscopes showed it efficacy on reducing dirtiness (p<0,001) and microbial contamination level (p=0,03), including a small percentage of culturable microorganism. From 75 endoscopes tested after reprocessing demonstrated dirtiness level reduction (all samples <50RUL, from internal and external area); but, also, presence of bacterial load of 3 log of bacteria/mL and 10,6% of culture positive. The microorganisms isolated from endoscope flush after reprocessing were Staphylococcus aureus, Staphylococcus epidermidis, Staphylococcus hominis, Staphylococcus capitis, Roseomonas gilardii and Micrococcus luteus. Endoscope biopsy channel analysis showed that samples from Brazil had higher contamination level than the ones from Australia (p<0,001). All channels analyzed presented biofilm and damage on their surfaces, however Brazilian samples showed particularities, like, blood cells, neutrophils and fungus. So, ever with low microbial load on endoscopes there is a potential risk of crossinfection associated with biofilm, compromising reprocessing quality and safety. Additionally, there´s the need of scientific and technologic improvement on endoscope reprocessing against biofilm

Lei, Man Cheong. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2011. / Includes bibliographical references (leaves 79-80). / Abstracts in English and Chinese. / Abstract --- p.i / Acknowledgement --- p.iii / Table of Contents --- p.iv / Chapter 1. --- Introduction --- p.1 / Chapter 2. --- Introduction to Ureteroscope --- p.3 / Chapter 2.1. --- Ureteroscope --- p.3 / Chapter 2.2. --- Configuration of flexible ureteroscope --- p.5 / Chapter 2.2.1. --- The optical system --- p.5 / Chapter 2.2.2. --- Deflection mechanism --- p.6 / Chapter 2.2.3. --- Control wires (Guidewires) --- p.9 / Chapter 2.2.4. --- Working channel --- p.10 / Chapter 2.3. --- Problems of the existing products --- p.10 / Chapter 2.4. --- Designs of the bending section --- p.11 / Chapter 2.4.1. --- Pin-joint bending section --- p.12 / Chapter 2.4.2. --- Wire-connected bending section --- p.15 / Chapter 2.5. --- CAD Modeling of the bending section --- p.16 / Chapter 2.5.1. --- Different designs of components --- p.16 / Chapter 2.5.2. --- Configuration of the bending section --- p.17 / Chapter 3. --- Wire-Driven Deflection Mechanism --- p.19 / Chapter 3.1. --- Literature review --- p.19 / Chapter 3.2. --- Geometry modeling of the bending section --- p.21 / Chapter 3.2.1. --- Description of formulation --- p.21 / Chapter 3.2.2. --- Assumption --- p.21 / Chapter 3.2.3. --- Notation --- p.22 / Chapter 3.2.4. --- Geometric analysis --- p.22 / Chapter 3.3. --- Simulations of the motion of the deflection mechanism --- p.26 / Chapter 3.3.1. --- Construction of the simulation --- p.26 / Chapter 3.3.2. --- Controlled by a pair of wires --- p.28 / Chapter 3.3.2.1. --- For bending section composed by identical parts --- p.28 / Chapter 3.3.2.2. --- For bending section composed by two sub-sections --- p.29 / Chapter 3.3.3. --- Controlled by two pairs of wires --- p.30 / Chapter 3.3.3.1. --- Deflecting in the same direction --- p.30 / Chapter 3.3.3.2. --- Deflecting in opposite direction --- p.31 / Chapter 3.4. --- Trajectory of the distal end --- p.32 / Chapter 3.4.1. --- Bending section composing by identical parts --- p.32 / Chapter 3.4.2. --- Bending section composing by two sub-sections --- p.40 / Chapter 3.4.2.1. --- Controlled by a single pair of wires --- p.43 / Chapter 3.4.2.2. --- Controlled by two pairs of wires --- p.46 / Chapter 3.5. --- Static analysis of the deflection mechanism --- p.57 / Chapter 4. --- Application: Design of Ureteroscope --- p.64 / Chapter 4.1. --- Design of the bending section --- p.64 / Chapter 4.2. --- Design of the control body --- p.67 / Chapter 4.2.1. --- Parts introduction and major assembling --- p.68 / Chapter 4.2.2. --- Control mechanism --- p.72 / Chapter 5. --- Conclusion and Future Work --- p.75 / Chapter 6. --- Bibliography --- p.79 / Chapter Appendix A: --- List of Publication --- p.81 / Chapter Appendix B: --- MATLAB Programs V --- p.83

Made available in DSpace on 2014-07-29T15:04:28Z (GMT). No. of bitstreams: 1 dissertacao-jackeline-maciel.pdf: 555827 bytes, checksum: e5c0f7c6020e05758b2a38bfe2ab0aa0 (MD5) Previous issue date: 2008-04-24 / SUMMARY: This is a descriptive research carried out in twenty digestive endoscopy services at the municipality of Goiânia. We aimed at characterizing the endoscope reprocessing through the use of glutaraldeide in upper digestive endoscopy services. The sample was constituted by endoscopes used for upper digestive endoscopy and professionals performing these endoscope reprocessing. We observed ethical legal aspects of human research. Data was collected through direct observation of the physical structure of the place used for endoscope reprocessing and the individuals performing such reprocessing and recorded in a check list. In each service we obtained samples of three endoscopes, able to be chosen the first, the second, and the last endoscope used in that period. Samples were collected in the border, biopsy channel, and aspiration channel of each endoscope right after the use and at the end of reprocessing. Samples were processed in the Laboratório de Bacteriologia Médica of the Instituto de Patologia Tropical e Saúde Pública of the Universidade Federal de Goiás. Microorganisms were identified concerning the form through Gram coloring technique. For isolated bacteria we performed the susceptibility profile. We carried out twenty observations regarding physical structure, one in each service. Sixty endoscope reprocessing were observed, three in each service. The outcomes showed that reprocessing is carried out in the same screening room without proper flow of service. All reprocessing stages showed failure. Errors were observed during the cleansing stage due to the use of enzyme detergent and brushing only the biopsy tubes. Disinfection was identified in all reprocessing, however it was not observed at 2 % concentration of germicide, there was no aspiration of the product in the internal channels and total immersion of the endoscopes, and exposure time to glutaraldeide was lower than the recommended one. Rinsing was most of the time with unfiltered water. The internal channels drying was improper due to the nonuse of compressed air. Adequate conditions to endoscope storage were identified. Microbial range of contaminated endoscopes was low, between 101 a 104 ufc/mL. Performing the precleansing stage, the use of enzyme detergent, cleansing of internal endoscope tubes, and the filling of these tubes with glutaraldeide were the significant variables, < 0.005 for the efficiency of endoscope reprocessing. These stages were crucial to reduce microbial burden. Chemical disinfection through the use of glutaraldeide was efficient in the endoscope reprocessing in eight services which is evidenced through the elimination of initial microbial range, however failures in the reprocessing of those were identified. We isolated from decontaminated endoscope: S. aureus, Staphylococcus coagulase negative, Enterobacter agglomerans, Enterobacter sp., E. coli. These microorganisms were susceptible to antimicrobials tested, except for azitromycin-resistant Gram-negative bacteria. This study shows that despite the low microbial range of contaminated endoscopes, disinfection has not been achieved in all services due to reprocessing failures, mainly in the cleaning stage. We consider adherence to endoscopes reprocessing protocols eliminates the major failures identified in the reprocessing / Pesquisa descritiva realizada em vinte Serviços de Endoscopia Digestiva do Município de Goiânia. Objetivou caracterizar o reprocessamento dos endoscópios pelo uso do glutaraldeído em serviços de endoscopia. A amostra constituiu-se de endoscópios utilizados para Endoscopia Digestiva Alta, e de profissionais que realizavam os reprocessamentos. Foram observados os aspectos ético-legais de pesquisa, em seres humanos. Os dados foram obtidos mediante observação direta da estrutura física do local utilizado para o reprocessamento dos endoscópios, e dos sujeitos que realizaram este reprocessamento, e registrados em um check-list. Em cada serviço foram obtidas amostras de três endoscópios, sendo elegíveis o primeiro, o segundo e o último endoscópio utilizados, no período. As amostras foram coletadas na ponta, canal de biópsia e canal de aspiração, de cada endoscópio, logo após o uso, e ao término do reprocessamento. As amostras foram processadas no Laboratório de Bacteriologia Médica do Instituto de Patologia Tropical e Saúde Pública da Universidade Federal de Goiás. Os microrganismos foram identificados quanto à forma, pela técnica de coloração de Gram. Para as bactérias isoladas foi realizado o perfil de suscetibilidade. Foram realizadas 20 observações referentes à estrutura física, uma em cada serviço. Foram observados 60 reprocessamentos de endoscópios, sendo três em cada serviço. Os resultados mostraram que o reprocessamento é realizado na mesma sala de exames, sem atendimento de um fluxo adequado. Houve falhas em todas as etapas do reprocessamento. Foram constatadas deficiências, durante a etapa de limpeza, por uso inadequado do detergente enzimático e escovação apenas do canal de biópsia. A desinfecção foi executada em todos os reprocessamentos, contudo verificada concentração inferior a 2% do germicida, não houve aspiração do produto nos canais internos, e imersão total do endoscópio, e o tempo de exposição ao glutaraldeído inferior ao recomendado. O enxágüe, na maioria das situações, ocorreu com o uso de água sem filtrar. Secagem dos canais internos é inadequada pela falta de uso de ar comprimido. Condições adequadas para o armazenamento do endoscópio foram confirmadas. A carga microbiana dos endoscópios contaminados foi baixa, entre 101 e 104 ufc/mL. A realização da pré-lavagem, o uso do detergente enzimático, a limpeza dos canais internos do endoscópio, e o preenchimento destes canais com o glutaraldeído foram variáveis significativas, < 0,005 para a eficácia do reprocessamento dos endoscópios. Estas etapas foram determinantes para a redução da carga microbiana. A desinfecção química pelo glutaraldeído foi eficaz no reprocessamento dos endoscópios em oito serviços evidenciada pela eliminação da carga microbiana inicial, embora houvesse falhas no reprocessamento. Para os endoscópios reprocessados isolamos: S. aureus, Staphylococcus coagulase negativa, Enterobacter agglomerans, Enterobacter sp., E. coli. Estes microrganismos foram suscetíveis aos antimicrobianos testados, com exceção das bactérias Gram-negativas resistentes à azitromicina. O estudo mostra que apesar da baixa carga microbiana dos endoscópios imediatamente após o uso, a desinfecção não foi alcançada, em todos os serviços, por falhas no reprocessamento, especialmente na etapa de limpeza. Consideramos que, a adesão aos protocolos de reprocessamento dos endoscópios eliminaria as principais falhas identificadas.

Hintergrund: Die Osteotomie von Weisheitszähnen ist im zahnärztlichen Alltag ein Routineverfahren. Das primäre Ziel der modernen Operationsverfahren ist die Reduktion operationsbedingter Morbidität und der Erhalt von umgebendem Knochen. Wir beschreiben eine Technik, durch die über einen okklusalen Zugang impaktierte Weisheitszähne mit Mini-Flap und ohne laterale Osteotomie entfernt werden können. Methode: Nach okklusaler Freilegung des Knochens im Bereich des dritten Molaren erfolgt die Visualisierung des Operationsfeldes mit Hilfe eines Stützendoskops (30 Grad Vorausblick, 2,7mm Durchmesser, Fa. Storz, Tuttlingen) und Mikroskops (40-fache Vergrößerung, Zeiss OPMI). Unter vergrößernder Betrachtung des Operationsfeldes mit dem Stützendoskop und Mikroskop wird die Krone schrittweise separiert und fragmentiert. Dabei wird eine raumschaffende Präparation im Sinne einer Implosionstechnik angewendet. Kronenfragmente und Wurzeln werden durch die okklusale Kavität entfernt. Ergebnisse: Bei 40 Patienten (19 Männer, 21 Frauen, Alter: 15-55) wurden 50 Weisheitszähne uni- oder bilateral entfernt. Die mittlere Operationszeit betrug 18,41min (4-89,5min), wobei sich zwischen den einzelnen Angulationstypen kein signifikanter Unterschied zeigte (vertikal -22,58min, horizontal -21,55min, mesial -16,11min, distal -12,10min). Der durchschnittliche Knochenverlust betrug ca. 2mm (0,4-6,5mm), wobei eine gleichmäßige Verteilung in allen vier Angulationstypen zu ermitteln war. Intraoperative Komplikationen traten nicht auf (0%). In sechs Fällen (12%) wurden postoperative Komplikationen beobachtet, drei temporäre Hyp-oder Parästhesien (6%), zwei Entzündungen (4%) und ein verbliebener Wurzelrest (2%). Schlussfolgerung: Die okklusale Expositionstechnik mit endoskopischer und mikroskopischer Visualisierung erlaubt unter Verzicht einer großflächigen Lappenbildung eine gezielte und schonende Zahnseparation. Das gilt insbesondere für die Entfernung komplex verlagerter Zähne mit enger Lagebeziehung zum Nervus alveolaris inferior. Die okklusale Expositions-technik ist auch bei Zähnen mit unmittelbarem Nervenkontakt anwendbar. Bei der Weisheitszahnentfernung und analog auch in anderen Kieferregionen kann diese Methode Knochenverluste und großflächige Gewebetraumata vermeiden. Dem Nachteil eines erhöhten Zeitaufwandes stehen eine hohe Präzision der Op-Techniken und eine bessere Übersicht in komplexen Fällen positiv gegenüber.

Submitted by Cássia Santos (cassia.bcufg@gmail.com) on 2017-04-03T12:00:58Z No. of bitstreams: 3 Tese - Cristiana da Costa Luciano - 2016 - parte 1.pdf: 9498921 bytes, checksum: 226b340d0cc04924992dc645bdc6a8ad (MD5) Tese - Cristiana da Costa Luciano - 2016 - parte 2.pdf: 14789773 bytes, checksum: 89f6da4ebc9309b0d0527169ad37cc50 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) / Approved for entry into archive by Luciana Ferreira (lucgeral@gmail.com) on 2017-04-03T12:05:34Z (GMT) No. of bitstreams: 3 Tese - Cristiana da Costa Luciano - 2016 - parte 1.pdf: 9498921 bytes, checksum: 226b340d0cc04924992dc645bdc6a8ad (MD5) Tese - Cristiana da Costa Luciano - 2016 - parte 2.pdf: 14789773 bytes, checksum: 89f6da4ebc9309b0d0527169ad37cc50 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) / Made available in DSpace on 2017-04-03T12:05:34Z (GMT). No. of bitstreams: 3 Tese - Cristiana da Costa Luciano - 2016 - parte 1.pdf: 9498921 bytes, checksum: 226b340d0cc04924992dc645bdc6a8ad (MD5) Tese - Cristiana da Costa Luciano - 2016 - parte 2.pdf: 14789773 bytes, checksum: 89f6da4ebc9309b0d0527169ad37cc50 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Previous issue date: 2016-12-20 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES / INTRODUCTION: Flexible Gastrointestinal Endoscopes (FGE) are used for diagnostic and therapeutic procedures, classified as semicritical health products (HP), requiring High-Level Disinfection (HLD) processing among users. The FGE designs are complex, making it difficult to process and favor the occurrence of faults that contribute to the accumulation of organic matter on the surface of the internal channels of the endoscopes, contributing to the formation of the biofilm. OBJECTIVE: To develop a Biofilm Buildup (BBF) accumulation model, based on repeated exposure of test soil containing Entercoccus faecalis and Pseudomonas aeruginosa by means of repeated cycles of fixation and to evaluate the ability of detergents and disinfectants to destroy and remove bacteria in the Traditional Biofilm (TBF) and Buildup. MATERIALS AND METHODS: TBF was developed in MBEC ™ peg, without hydroxyapatite, and BBF, with hydroxyapatite, over a period of eight days. For the development of both biofilms, E. faecalis and P. aeruginosa, containing 8 log10, colony forming units per cubic centimeters (CFU/cm2) were used. Prolystica Enzymatic (D1), Prolystica Neutral (D2), Neodisher (D3) and Endozime (D4) were tested alone and in combination with Glutaraldehyde (GLUT), Orthophthaldehyde (OPA) and Hydrogen Peroxide Accelerator (APH) to determine if both biofilms could be removed. The removal of the traditional biofilm and buidulp, using viable bacteria count, quantification of protein and carbohydrates and by means of scanning electron microscopy (SEM) was evaluated. RESULTS: After eight days of BBF development, 6.14 log10 CFU/cm2 of E. faecalis and 7.71 log10 CFU/cm2 of P. aeruginosa were reached. None of the detergents and disinfectants have been able to remove the traditional biofilms and buildup or reduce the level of bacteria. The combination of detergents and disinfectants tested in BBF provided a reduction of 3 to 5 log10 in viable bacteria, but no combination could provide the expected reduction of l log10. Only enzyme Prolystica and Endozime removed both E. faecalis (3.90 log10 colony forming units per milliliter (CFU/mL)) and P. aeruginosa (3.96 log10 CFU/mL) in suspension bacteria. None of the detergents tested removed > 1 log10 CFU/cm2 from the bacteria within the traditional biofilm. No combination of high-level disinfectant and detergent reduced the level of both E. faecalis and P. aeruginosa from the traditional biofilm interior (3 to 5 log10 CFU/cm2). Although the combination of Endozyme and Glutaraldehyde reduced 6 log10, it did not eliminate both bacteria in the traditional biofilm. CONCLUSION: Data indicate that if TBF and BBF accumulate in the EGF channels during repeated processing cycles, neither detergents nor high level disinfectants will provide the expected level of bacterial removal or destruction. Future research using the buildup model can help develop new cleaning and disinfection methods that can prevent or eliminate the BBF within the endoscope channels. / INTRODUÇÃO: Endoscópios Gastrointestinais Flexíveis (EGF) são utilizados para procedimentos de diagnóstico e terapêutica, classificados como Produtos Para Saúde (PPS) semicríticos, que necessitam ser submetidos ao processamento de Desinfecção de Alto Nível (DAN) entre usuários. Os designs dos EGF são complexos, dificultando o processamento e favorecem a ocorrência de falhas que contribuem para o acúmulo de matéria orgânica na superfície dos canais internos dos endoscópios e para a formação do biofilme. OBJETIVO: Desenvolver um modelo de acumulação de Biofilme Buildup (BBF), baseado em exposição repetida de solo teste, contendo Entercoccus faecalis e Pseudomonas aeruginosa por meio de ciclos repetidos de fixação e avaliar a capacidade de detergentes e desinfetantes para destruir e remover Bactérias nos Biofilmes Tradicional (TBF) e buildup. MATERIAIS E MÉTODOS: O TBF foi desenvolvido em MBEC™ peg, sem hidroxiapatita, e o BBF, com hidroxiapatita, ao longo de oito dias. Para o desenvolvimento de ambos os biofilmes, utilizaram-se E. faecalis e P. aeruginosa, contendo 8 log10, unidades formadoras de colônias por centímetros cúbicos (UFC/cm2). Testaram-se os detergentes, Prolystica enzimática (D1), Prolystica Neutro (D2), Neodisher (D3) e Endozime (D4) isoladamente e em combinação com o Glutaraldeído (GLUT), Ortoftaldeído (OPA) e Acelerador Peróxido de Hidrogênio (APH) para determinar se ambos os biofilmes poderiam ser removidos. Avaliou-se a remoção dos biofilmes tradicional e buidulp, utilizando contagem de bactérias viáveis, quantificação de proteína e carboidratos e por meio da microscopia eletrônica de varredura (MEV). RESULTADOS: Após oito dias de desenvolvimento BBF, foram atingidos 6,14 log10 UFC/cm2 de E. faecalis e 7,71 log10 UFC/cm2 de P. aeruginosa. Nenhum dos detergentes e desinfetantes conseguiu remover os biofilmes tradicional e buildup ou reduzir o nível de bactérias. A combinação de detergentes e desinfetantes testada em BBF proporcionou uma redução de 3 a 5 log10 em bactérias viáveis, mas nenhuma combinação pôde proporcionar a redução esperada de log10. Apenas Prolystica enzimática e Endozime removeram ambos E. faecalis (3,90 log10 unidades formadoras de colônias por mililitros (UFC/mL) e P. aeruginosa (3,96 log10 UFC/mL) em bactérias em suspensão. Nenhum dos detergentes testados removeu > 1 log10 UFC/cm2 das bactérias dentro do biofilme tradicional. Nenhuma combinação de detergente e desinfetante de alto nível reduziu o nível de ambos E. faecalis e P. aeruginosa do interior de biofilme tradicional (3 a 5 log10 UFC/cm2). Embora a combinação de Endozime e Glutaraldeído reduziu 6 log10, não eliminou ambas as bactérias no biofilme tradicional. CONCLUSÃO: Os dados indicam que, se TBF e BBF acumularem nos canais de EGF durante ciclos repetidos de processamento, nem os detergentes nem os desinfetantes de alto nível irão fornecer o nível esperado de sua remoção ou destruição bacteriana. Pesquisas futuras, utilizando o modelo buildup, podem ajudar a desenvolver novos métodos de limpeza e desinfecção que consigam evitar ou eliminar a BBF dentro dos canais do endoscópio.