This project aims to find how, why and by which means the sense of smell can create meaningful interactions in the field of escape rooms, by taking a main role in the interactions that occur in said games, by adding an additional layer of complexity and embodiment. The project finished with the finding that it is not only possible to create meaningful interactions and expand on the possibilities of smell interaction, but also by encouraging future exploration and discussion of the sense of smell as an interactive medium and material.

The adoption of containers and container orchestration in cloud computing is motivated by many aspects, from technical and organizational to economic gains. In this climate, even security demanding organizations are interested in such technologies but need reassurance that their requirements can be satisfied. The purpose of this thesis was to investigate how separation of applications could be achieved with Docker and Kubernetes such that it may satisfy the demands of the Swedish Police Authority. The investigation consisted of a literature study of research papers and official documentation as well as a technical study of iterative creation of Kubernetes clusters with various changes. A model was defined to represent the requirements for the ideal separation. In addition, a system was introduced to classify the separation requirements of the applications. The result of this thesis consists of three architectural proposals for achieving segmentation of Kubernetes cluster networking, two proposed systems to realize the segmentation, and one strategy for providing host-based separation between containers. Each proposal was evaluated and discussed with regard to suitability and risks for the Authority and parties with similar demands. The thesis concludes that a versatile application isolation can be achieved in Docker and Kubernetes. Therefore, the technologies can provide a sufficient degree of separation to be used in security demanding environments. / Populariteten av containers och container-orkestrering inom molntjänster motiveras av många aspekter, från tekniska och organisatoriska till ekonomiska vinster. I detta klimat är även säkerhetskrävande organisationer intresserade av sådana teknologier men söker försäkran att deras kravbild går att möta. Syftet med denna avhandling var att utreda hur separation mellan applikationer kan nås vid användning av Docker och Kubernetes så att Polismyndighetens krav kan uppfyllas. Undersökningen omfattade en litterär studie av vetenskapliga publikationer och officiell dokumentation samt en teknisk studie med iterativt skapande av Kubernetes kluster med diverse variationer. En modell definierades för att representera kravbilden för ideal separation. Vidare så introducerades även ett system för klassificering av separationskrav hos applikationer. Resultatet omfattar tre förslag på arkitekturer för att uppnå segmentering av klusternätverk i Kubernetes, två föreslagna systemkomponenter för att uppfylla segmenteringen, och en strategi för att erbjuda värd-baserad separation mellan containers. Varje förslag evaluerades med hänsyn till lämplighet och risker för myndigheten och parter med liknande kravbild. Avhandlingens slutsats är att en mångsidig applikationsisolering kan uppnås i Docker och Kubernetes. Därmed kan teknologierna uppnå en lämplig grad av separation för att kunna användas för säkerhetskrävande miljöer.

The adaptive immune response forms an essential part of the cancer immuno-editing process, whereby nascent malignant cells are detected and destroyed prior to forming tumours. The process is tightly controlled to minimise collateral damage to healthy tissue. One of the mechanisms evolved for this purpose and frequently co-opted by malignant cells is the creation of a microenvironment scarce in essential amino-acids through the use of catabolic enzymes such as Indoleamine 2,3-dioxygenase (IDO) , responsible for the rate-limiting step in tryptophan catabolism. The evolutionary conserved GCN2 and mTORC1 pathways respond to amino-acid starvation by triggering emergency homeostatic response programmes that aim to conserve nutrients by shutting down biosynthetic pathways, slowing cell cycle progression and facilitating autophagy. This research project focuses on elucidating the interaction between IDO activity and these pathways and its implications for the immune-editing process. The role of the mTOR kinase as a regulator of T cell fate following exposure to cognate antigen has recently become apparent. Experiments described herein confirm that in murine and human models of T cell activation exposure to tryptophan starvation results in significant mTORC1 inhibition and a modified phenotype with reduced Tbet expression, altered cytokine secretion profile, greatly impaired proliferative capability and expanded CD4⁺ FoxP3⁺ CD25^high subpopulations. Additional results confirmed that the action of IDO is sufficient to deplete tryptophan from the microenvironment to levels sufficient to depress the mTORC1 axis and trigger GCN2 activity even in tumour cell lines. Lower extracellular tryptophan levels were necessary to perturb these pathways In IDO expressing cell lines, suggesting that compensatory mechanisms allow continued proliferation of malignant cells in the face of conditions that severely impede an anti-cancer immune response. In conclusion, manipulation of the mTORC1 axis via IDO-induced tryptophan depletion is an important tumour immune-escape mechanism that can be a target for cancer immunotherapies.

Cette thèse étudie l’Art poétique de Paul Claudel et son influence dans sa création avec un regard critique chinois. Pour éviter des interprétations excessives, nous élaborons une approche interculturelle. Dans le processus concret de la lecture, nous employons d’abord l’art de la critique des anciens esthètes chinois, résumons de manière synthétique notre ressenti intuitif inspiré par l’ensemble de l’œuvre de Claudel en deux sentiments : la marche et la fuite. Puis, nous faisons appel à la méthode traditionnelle occidentale de l’analyse textuelle et à la méthode de la recherche documentaire, afin de relier les effets aux textes. L’observation du développement et de la transformation de ces deux forces permet de traverser la complexité apparente de l’œuvre du poète, et de révéler l’influence directe et des convergences possibles entre sa pensée et la pensée orientale. / This thesis studies Art poétique of Paul Claudel and his influence in his creation with a Chinese critical perspective. To avoid excessive interpretation, I develop an intercultural approach. In the actual process of reading, I use the classical Chinese aesthetic and literary theories to summarize synthetically the aesthetic effect of the works as a whole, and proposes the two kinds of effects as “walk” and “escape”. Then I use the traditional western method of textual analysis and the method of documentary research, to link effects to text. The observation of the development and transformation of these two forces can cross the apparent complexity of the poet’s work, and reveal the direct influence and possible convergences between his thought and Eastern thought.

Em cana-de-açúcar (Saccharum spp.), a deficiência hídrica é um dos estresses ambientais mais danosos, e a seleção de genótipos com maior adaptação aos ambientes com tendência ao déficit hídrico seria de grande valor para a expansão da cultura para áreas consideradas marginais. O objetivo dessa dissertação foi investigar o mecanismo de resposta a seca presente em duas cultivares de cana-de-açúcar contrastantes, avaliando características fisiológicas juntamente com a discriminação isotópica de carbono (\'delta\' 13C) e o \"escape\" do CO2 (\'phi\'). Além disso, o efeito da seca na fotossíntese C4 foi analisado a fim de investigar a rota enzimática envolvida no transporte de ácidos de quatro carbonos entre as células do mesofilo e as células da bainha. Para tanto foram utilizados os genótipos contrastantes para a resposta à deficiência hídrica \'IACSP94-2094\' (tolerante) e \'IACSP97-7065\' (suscetível), cultivados em casa de vegetação, os quais foram selecionados entre 10 genótipos avaliados em experimentos fisiológicos em campo. As análises de \'delta\' 13C e \'phi\' mostraram variações significativas entre os genótipos, sendo observados maiores valores de \'delta\' 13C e \'phi\' na cultivar \'IACSP94-2094\' durante a seca. Além disso, foram conduzidas análises de expressão gênica e atividade enzimática referente as três variações da via fotossintética C4,que diferiram quanto ao ácido de quatro carbonos transportado entre o mesofilo e as células da bainha e quanto a enzima de descarboxilação presente nas células da bainha, incluindo a enzima málica dependente de NADP (NADP:ME), enzima málica dependente de NAD (NAD:ME) ou fosfoenolpiruvato carboxiquinase (PCK). Admite-se que o mecanismo de transporte de compostos de quatro carbonos presente na cana-de-açúcar seja aquele referente à NADP:ME, entretanto,o maior acúmulo do número de transcritos e maior atividade enzimática para PCK observadas na cultivar considerada tolerante (\'IACSP94 2094\') quando sob estresse, indicam que a via de descarboxilação do tipo PCK aparentemente poderia ser ativada em condições de déficit hídrico. Por outro lado, a ausência da indução da via PCKna cultivar considerada sensível (\'IACSP97 7065\'), sugere que esse mecanismo alternativo de descarboxilação poderia estar relacionadoà tolerância à seca. Essa hipótese pode ser reforçada pelos maiores valores de \'phi\' na \'IACSP94 2094\' quando em déficit hídrico, uma vez que o \'phi\' em plantas que possuem o tipo PCK foi maior que os valores encontrados em plantas do tipo NADP:ME. Dessa forma,nossos dados sugerem que o mecanismo alternativo de descarboxilação dependente de PCK, é induzido pelo déficit hídrico, e poderia ser adotado para a seleção de genótipos tolerantes à seca / Drought stress in sugarcane (Saccharum spp.) is one of the most harmful environmental stresses causing yield losses. To develop drought tolerant cultivars, the selection of genotypes with improved adaptation to this adverse environmental condition are highly required for sugarcane breeding programs. The objective of this dissertation was to investigate drought responses mechanism present in sugarcane by evaluation of physiological measurements, carbon isotope discrimination (\'delta\' 13C) and leakage of CO2 (\'phi\') between two contrasting sugarcane cultivars to drought stress. Further, the effect of drough on C4 photosynthetic mechanism was analized to inquire the enzymatic pathway involved in the transport of the C4 acids from mesophyll cells to the bundle sheath cells. Through physiological experiments, two contrasting cultivars, \'IACSP94 2094\' (tolerant) and \'IACSP97 7065\' (sensitive) to water deficit were screened among 10 cultivars submmited to drought prone field conditions. The \'delta\'13 C and \'phi\' analysis showed significant variation between the two cultivars during drought growth condictions wherein \'IACSP94-2094\' cultivar displayed high \'delta\' 13 C and \'phi\' rates. A quantitative expression profile and enzymatic activity were analyzed considering the three alternative pathways of C4 decarboxylation, NADP-dependent malic enzyme (NADP:ME), NAD dependent malic enzyme (NAD:ME) or phosphoenolpyruvatecarboxykinase (PCK) during drought stress. Despite the NADP-ME C4 mechanism present in Sugarcane, the increased expression and activity levels of PCK decarboxylation pathway was shown in the tolerant cultivar (\'IACSP94 2094\') during drought. However, the drought sensitive cultivar lacks the ability to triggers such a decarboxylation mechanism which suggest that this alternative mechanism might be associated with drought tolerance. The increased \'phi\' in PCKtype C4 plants was detected over the NADP:ME-type C4 plants which supports the presence of this alternative decarboxylation process. Altogether, the data presented here suggest that the PCK mechanism of decarboxylation is induced by drought stress and can be used to select drought tolerant genotypes

As energias e larguras de escape das Ressonâncias Gigantes, são calculadas utilizando-se técnicas de projeção visando incluir as ressonâncias de partícula-única nos cálculos de RPA (\"Random Phase Approximation\"). As equações de RPA obtidas são complexas, e as larguras de escape são interpretadas como sendo a parte imaginaria das soluções em energia. Os cálculos são feitos para a RGE1( O16 ) e RGE0 ( Zr90 ). Para o Zr90, os espectros das partículas emitidas pela RGE0, prótons e nêutrons, são calculados através do modelo híbrido. Devido à existência de mais do que um tipo de partícula sendo emitida, as analises são feitas dentro da aproximação de independência entre os canais de partículas no formalismo do modelo híbrido. / The energy and the escape of the Giant Resonances are calculated utilizing the projection to include the single-particle resonances in complex RPA (Random Phase Approximation) calculations. The escape widths are interpreted as the imaginary parts of the energy solution of the RPA equations. The Calculations are performed for the RGE1 ( O16 ) and RGE0 ( Zr90 ). For the Zr90 the spectra of the particles ejected by the RGE0, protons and neutrons, are calculated within the hybrid model. The analysis are made through the channels independence in the hybrid model formalism.

Em cana-de-açúcar (Saccharum spp.), a deficiência hídrica é um dos estresses ambientais mais danosos, e a seleção de genótipos com maior adaptação aos ambientes com tendência ao déficit hídrico seria de grande valor para a expansão da cultura para áreas consideradas marginais. O objetivo dessa dissertação foi investigar o mecanismo de resposta a seca presente em duas cultivares de cana-de-açúcar contrastantes, avaliando características fisiológicas juntamente com a discriminação isotópica de carbono (\'delta\' 13C) e o \"escape\" do CO2 (\'phi\'). Além disso, o efeito da seca na fotossíntese C4 foi analisado a fim de investigar a rota enzimática envolvida no transporte de ácidos de quatro carbonos entre as células do mesofilo e as células da bainha. Para tanto foram utilizados os genótipos contrastantes para a resposta à deficiência hídrica \'IACSP94-2094\' (tolerante) e \'IACSP97-7065\' (suscetível), cultivados em casa de vegetação, os quais foram selecionados entre 10 genótipos avaliados em experimentos fisiológicos em campo. As análises de \'delta\' 13C e \'phi\' mostraram variações significativas entre os genótipos, sendo observados maiores valores de \'delta\' 13C e \'phi\' na cultivar \'IACSP94-2094\' durante a seca. Além disso, foram conduzidas análises de expressão gênica e atividade enzimática referente as três variações da via fotossintética C4,que diferiram quanto ao ácido de quatro carbonos transportado entre o mesofilo e as células da bainha e quanto a enzima de descarboxilação presente nas células da bainha, incluindo a enzima málica dependente de NADP (NADP:ME), enzima málica dependente de NAD (NAD:ME) ou fosfoenolpiruvato carboxiquinase (PCK). Admite-se que o mecanismo de transporte de compostos de quatro carbonos presente na cana-de-açúcar seja aquele referente à NADP:ME, entretanto,o maior acúmulo do número de transcritos e maior atividade enzimática para PCK observadas na cultivar considerada tolerante (\'IACSP94 2094\') quando sob estresse, indicam que a via de descarboxilação do tipo PCK aparentemente poderia ser ativada em condições de déficit hídrico. Por outro lado, a ausência da indução da via PCKna cultivar considerada sensível (\'IACSP97 7065\'), sugere que esse mecanismo alternativo de descarboxilação poderia estar relacionadoà tolerância à seca. Essa hipótese pode ser reforçada pelos maiores valores de \'phi\' na \'IACSP94 2094\' quando em déficit hídrico, uma vez que o \'phi\' em plantas que possuem o tipo PCK foi maior que os valores encontrados em plantas do tipo NADP:ME. Dessa forma,nossos dados sugerem que o mecanismo alternativo de descarboxilação dependente de PCK, é induzido pelo déficit hídrico, e poderia ser adotado para a seleção de genótipos tolerantes à seca / Drought stress in sugarcane (Saccharum spp.) is one of the most harmful environmental stresses causing yield losses. To develop drought tolerant cultivars, the selection of genotypes with improved adaptation to this adverse environmental condition are highly required for sugarcane breeding programs. The objective of this dissertation was to investigate drought responses mechanism present in sugarcane by evaluation of physiological measurements, carbon isotope discrimination (\'delta\' 13C) and leakage of CO2 (\'phi\') between two contrasting sugarcane cultivars to drought stress. Further, the effect of drough on C4 photosynthetic mechanism was analized to inquire the enzymatic pathway involved in the transport of the C4 acids from mesophyll cells to the bundle sheath cells. Through physiological experiments, two contrasting cultivars, \'IACSP94 2094\' (tolerant) and \'IACSP97 7065\' (sensitive) to water deficit were screened among 10 cultivars submmited to drought prone field conditions. The \'delta\'13 C and \'phi\' analysis showed significant variation between the two cultivars during drought growth condictions wherein \'IACSP94-2094\' cultivar displayed high \'delta\' 13 C and \'phi\' rates. A quantitative expression profile and enzymatic activity were analyzed considering the three alternative pathways of C4 decarboxylation, NADP-dependent malic enzyme (NADP:ME), NAD dependent malic enzyme (NAD:ME) or phosphoenolpyruvatecarboxykinase (PCK) during drought stress. Despite the NADP-ME C4 mechanism present in Sugarcane, the increased expression and activity levels of PCK decarboxylation pathway was shown in the tolerant cultivar (\'IACSP94 2094\') during drought. However, the drought sensitive cultivar lacks the ability to triggers such a decarboxylation mechanism which suggest that this alternative mechanism might be associated with drought tolerance. The increased \'phi\' in PCKtype C4 plants was detected over the NADP:ME-type C4 plants which supports the presence of this alternative decarboxylation process. Altogether, the data presented here suggest that the PCK mechanism of decarboxylation is induced by drought stress and can be used to select drought tolerant genotypes

Tese de mestrado integrado. Engenharia Mecânica. Faculdade de Engenharia. Universidade do Porto. 2010

This poetry manuscript explores themes of family and addiction, education and New Orleans, and fear and escape in the span of three parts. Its preface examines the poet’s background and influences, his relationship with performance and humor, and the levity he hopes to create in his work.

The Alexander terrane is an allochthonous terrane within the North American Cordillera. New structural mapping and geochronology within the southern Alexander terrane in the vicinity of Porcher Island provides evidence for two major tectonic events. The oldest is Late Silurian to Early Devonian magmatism and deformation assigned to the Klakas orogeny. The area has subsequently been affected by mid-Cretaceous conjugate shear zones potentially associated with tectonic escape. Northwest-striking sinistral shear zones characterize mid-Cretaceous deformation in the western Coast Belt south of Prince Rupert in north coastal British Columbia. Structurally focused mapping and geochronology has revealed a component of lateral extension to this deformation. General flow characteristics of the shear zones are identified by comparison of fabric patterns to published models for fabric development in shear zones. U-Pb ages from synkinematic dykes constrain motion on northwest-striking sinistral transpressional shear zones, including the Useless, Barrett and Salt Lagoon shear zones, to ca. 104 – 96 Ma, and dextral transpression on the north-striking Telegraph Passage shear zone to ca. 97.6 ± 0.2 Ma. The geometry, kinematics, and coeval nature of these shear zones suggests that they formed in part as a ductile conjugate set. The presence of similarly-oriented conjugate shear bands in the apex zone between sinistral and dextral shear zones further reinforces this interpretation. The orientation of these conjugate sets indicates a component of north-northwest east-southeast extension. The conjugate shear zones merge together into the Grenville Channel shear zone, a sinistral transpressional shear zone with high strike-parallel stretch. A U-Pb age of 103 ± 32 Ma from a synkinematic dyke in the Grenville Channel shear zone coincides with a previously published Lu-Hf age of 102.6 ± 3.7 Ma on synkinematic garnet. Overall, structural and geochronological data from Porcher Island and surrounding area in north coastal British Columbia indicate that mid-Cretaceous deformation was characterized by ENE-WSW (orogen normal) shortening and NNW-SSE (orogen parallel) extension. This local strain regime is consistent with large-scale mid-Cretaceous tectonic escape as proposed for the northern Cordillera at that time, expressed in coeval sinistral faulting in the Coast Belt and dextral faulting in the northern Omineca belt. The Late Silurian to Early Devonian Ogden Channel complex is a mafic to intermediate metaplutonic-metamorphic complex within the southern Alexander terrane on southern Porcher Island and adjacent Pitt Island in north coastal British Columbia. Lithological characteristics of the complex suggest that it represents the mid-crustal roots of a volcanic arc. An age of 413.3 ± 2.5 Ma from a comparatively weakly deformed quartz diorite dyke indicates that the synkinematic Ogden Channel complex is at least in part Early Devonian in age, corresponding to the Klakas orogeny that affected the Alexander terrane in southeast Alaska. Crosscutting relationships indicate that individual intrusions within the Ogden Channel complex were emplaced syn- to post-kinematically with respect to southwest-vergent sinistral reverse deformation (present coordinates). The structural and lithological characteristics of the Ogden Channel complex are consistent with the interpretation that this part of the Alexander terrane was located in the upper plate of a northeast-dipping subduction zone, which culminated in the Klakas orogeny.