O presente estudo teve como objetivo desenvolver uma nova proposta de uso de um biomonitor na identificação de fontes emissoras de metais no meio ambiente. Foi selecionada a espécie de siri Callinectes danae como biomonitor e o estuário de Santos como área de estudo. Numa primeira etapa e considerando que o siri é um organismo bentônico, foi realizada uma avaliação preliminar do teor de metais no sedimento do estuário. Em seguida, foi realizada uma avaliação preliminar do teor de metais nos diferentes tecidos de indivíduos coletados na região (brânquias, hepatopâncreas e músculos). Baseado nos experimentos anteriores, foi proposto um modelo de distribuição dos metais entre os tecidos e destes em relação aos sedimentos. A validação deste modelo de distribuição (assinatura química) foi realizada por meio de um conjunto de procedimentos que visaram testar: 1) a robustez em função do tempo; 2) a especificidade para a região de estudo; 3) a sensibilidade às alterações dos níveis de metais em cada tecido. A partir deste modelo, foram identificados indícios para atribuição de fontes emissoras de metais. Desta forma, concluiu-se que, para a região do estuário de Santos, o siri da espécie C. danae pode ser utilizado como um biomonitor. O modelo proposto foi eficaz, uma vez que foi capaz de responder de forma conclusiva-positiva a todos os testes realizados na sua validação, confirmando esta espécie como um biomonitor definitivo para região. A robustez do modelo irá aumentar com novas coletas e a realimentação do banco de dados. / This study aimed to develop a new methodology for the use of a biomonitor in the identification of metal discharges in environmental evaluations. Crabs of Callinectes danae species were used in an evaluation conducted in the Santos Estuarine System. In the first experiment and since C. danae is a benthic species, a preliminary assessment of the metal concentrations in sediment samples collected in the Santos Estuarine System was performed. Subsequently, a preliminary assessment of metal concentrations in the C. danae tissues was also peformed. The last experiment aimed to identify a chemical fingerprint for the Santos Estuarine System.The development of validation procedures for this model (chemical fingerprint) was conducted and aimed to test: 1) the stability of this model through time; 2) its local specificity for the Santos Estuarine System; 3) the sensibility of this model due to modifications in the metal concentrations in each tissue (gills, hepatopancreas and muscles). By the use of this model, it was possible to identify the sources of metal emissions. According to the results, C. danae can be used as a biomonitor for the Santos Estuarine System. The established model was able to responde in a positive-conclusive way to all the tests performed in its validation, confirming this species as a definitive biomonitor for this area. Thus, the stability of this model will increase with new sampling campaings and consequently introduction of new information in the database.

A Formação Tombador, Mesoproterozoico, compreende diferentes sistemas deposicionais, depositados em um bacia sag, que abrangem desde sistemas aluviais a estuarinos. Os depósitos bem preservados e sua ampla ocorrência em escala regional (~300 km) faz com que a Formação Tombador seja um excelente caso de estudo no Proterozoico. Foram reconhecidas três sequências deposicionais, limitadas por superfícies erosivas em escala regional no topo da Formação Tombador. A Sequência I é composta na base por canais fluviais cascalhosos entrelaçados rasos, que são sotopostos por depósitos de dunas e lençóis de areia eólicos e inundações em lençol intermediário. O limite inferior desta sequência é caracterizado por uma discordância angular intra-Tombador sobre os sistemas fluvio-estuarinos, evidenciada por uma mudança abrupta de fácies e mudança nas paleocorrentes. Os sistemas fluvio-estuarinos abaixo da discordância apresentam paleocorrentes para noroeste enquanto que os sistemas fluviais acima do limite de sequências indicam um transporte para sul. Uma nova entrada abrupta de depósitos conglomeráticos relacionados a sistemas de leques aluviais sobre a sucessão fluvio-eólica, marca o limite da Sequência II. A Sequência III é caracterizada por sistemas fluvio-estuarinos na porção superior da Formação Tombador, que são progressivamente sucedidos por sistemas marinhos rasos (Formação Caboclo), definindo uma tendência geral transgressiva. As Sequências I e II refletem um soerguimento da área-fonte em resposta a movimentações tectônicas. A mudança abrupta de paleocorrentes dos fluviais basais da Sequência I indicam uma reestruturação regional das redes de drenagens, enquanto que os sistemas de leques aluviais da Sequência II sugerem sedimentos depositados por uma tectônica sin-deposicional. Os limites de sequências II e III é marcado por uma superfície erosiva regional. A discordâncias entre as sequências II e III revela um hiato significante no topo da Formação Tombador sugerindo uma origem tectônica para esta discordância. / The Mesoproterozoic Tombador Formation encompasses different depositional systems deposited in a sag basin, ranging from estuarine to alluvial. The well preserved deposits and their wide occurrence in the regional scale (~300 km) define the Tombador Formation as an excellent case study for the depositional patterns prevailing during the Proterozoic. Three depositional sequences were recognized for the Upper Tombador Formation, bounded by three semi-regional scale unconformities. Sequence I is composed of shallow, gravel-bed braided channels at its base, which are overlain by fine- to coarse-grained sandstones related to aeolian sand sheets and dunes and intermediate sheetfloods. The lower boundary of this sequence is characterized by an angular unconformity cutting fluvio-estuarine deposits, evidenced by an abrupt change of facies and fluvial palaeocurrents. The fluvio-estuarine deposits below the sequence boundary display palaeocurrents to northwest, whereas the fluvial strata above the unconformity show southeastward palaeocurrents. A new abrupt entrance of conglomeratic deposits related to alluvial fans systems overlying the fluvio-aeolian successions marks the lower boundary of Sequence II. The Sequence III is characterized by fluvio-estuarine systems in the top of the Upper Tombador Formation, that are progressively covered by shallow marine systems (Caboclo Formation), defining a general transgressive trend. The pattern of sequences I and II probably reflects the uplift of source areas in response to tectonic movements. The palaeocurrent change in Sequence I indicates a regional rearrangement of the drainage networks, while the alluvial fan systems of sequence II suggest sin-depositional tectonic pulses. The regional erosive surface between sequences II and III reveals a significant hiatus close to the Tombador Formation top, what suggests a tectonic origin for this unconformity.

Cities are usually associated to development and environmental impacts caused by urban settlements. The increase in population density also means growth in waste generated, mainly due to municipal sewage. In the case of Aracaju city, data indicate that only 31.8% of household waste are collected and the remainding is discarded in natura in drainage channels and in estuaries. In this environment, organic matter undergoes biodegradation in conditions quite different from those as freshwater medium, especially with regard to osmotic pressure, which varies under the influence of tides (salinity). This study aimed to study the biodegradation of municipal sewage in estuarine environment in the laboratory scale, to assess the environmental impact associated with the disposal of effluents in this medium. The salinities tested were: 1, 2 and 3.5%, the concentrations of organic matter (expressed in terms of BOD) were 150, 200 and 250 mg/L, using real water (water collected in the estuary. The control parameters measured during the experiments were the concentration of dissolved oxygen, pH and temperature and the parameters analyzed in the samples were total suspended solids and biochemical oxygen demand (BOD), according to Clesceri et al. (1998). The results showed that the microorganisms used are classified as halophilous. The kinetic coefficients indicate that, for salinities around 2%, the growth rate is maximum, and the substrate rate consumption, but these values are low compared to the freshwater matrix coefficients. The speed of growth decreases with the increase of concentration substrate, confirming that the kinetics is a Monod type. To conclude, the salt concentration affects significantly the microbial growth and the substrate, even in the situations where the microorganisms are adapted to the environmental conditions the natural reconstruction process of an estuary is slow, if ceased pollution sources. Therefore, there is a great necessity to develop public policies for environmental sanitation in Aracaju city (SE). / As cidades normalmente estão associadas a desenvolvimento e a impactos ambientais, ocasionados pelos aglomerados urbanos. O aumento da densidade demográfica implica também no crescimento dos resíduos gerados, principalmente os efluentes sanitários. No caso da cidade de Aracaju, dados indicam que apenas 31,8% dos efluentes domésticos são coletados e o restante é descartado in natura em canais e deságuam nos estuários. Nesse ambiente, a matéria orgânica sofre biodegradação em condições bem diferentes das de um meio doce, sobretudo no tocante à pressão osmótica, que varia sob o efeito das marés (salinidade). O presente trabalho teve como objetivo estudar a biodegradação de efluentes sanitários em meio estuarino, em escala de laboratório, para avaliar o impacto ambiental associado ao descarte de efluentes em tal atributo. As salinidades testadas foram: 1, 2 e 3,5%; as concentrações de matéria orgânica (expressas em termos da DBO) foram 150, 200 e 250 mg/L, em matrizes com água real (água coletada no próprio estuário). Os parâmetros de controle medidos ao longo dos experimentos foram a concentração de oxigênio dissolvido, pH e temperatura, e as análises realizadas nas amostras foram sólidos suspensos totais e Demanda Bioquímica de Oxigênio (DBO), segundo metodologia estabelecida por Clesceri et al. (1998). Os resultados obtidos mostraram que os microrganismos utilizados são classificados como halófilos. Os coeficientes cinéticos indicam que, para salinidades de 2%, a velocidade de crescimento é máxima, assim como a taxa de consumo do substrato, mas são valores baixos comparados aos coeficientes obtidos em experimentos em matrizes com água doce. A velocidade de crescimento aumenta até determinada concentração de substrato, a partir da qual a velocidade específica de crescimento fica constante, confirmando que a cinética é do tipo Monod. Concluiu-se então que a concentração de sal afeta significativamente o crescimento microbiano e o consumo do substrato. Mesmo no ambiente em que os microrganismos estejam adaptados às condições do meio, o processo de recuperação natural de um estuário é lento, caso sejam cessadas as fontes de poluição. Portanto, há necessidade de se elaborar políticas públicas voltadas para o saneamento ambiental no Município de Aracaju/SE.

Made available in DSpace on 2016-08-17T15:00:40Z (GMT). No. of bitstreams: 1 Dissertacao - PamellaSilvaBrito.pdf: 2572284 bytes, checksum: 775b92e1aa430b94c10075867a76b869 (MD5) Previous issue date: 2014-08-29 / FUNDAÇÃO DE AMPARO À PESQUISA E AO DESENVOLVIMENTO CIENTIFICO E TECNOLÓGICO DO MARANHÃO / The marine phanerogams meadows are important because they are nursery areas for many species of fish. In order to describe the community structure, temporal and spatial patterns of fish using Spartina alterniflora meadows of Maranhão Island present in tide macro region were held six samples of nine points in the period between July 2012 and June 2013, using a trawl net. A total of 11,660 individuals captured, representing 80 species, in which 13 are new record for the state of Maranhao. The sampled points are represented by a few abundant species (Rhinosardinia bahiensis, Atherinella brasiliensis, Lycengraulis grossidens, Lycengraulis batesii, incilis Mugil and Centegraulis edentulus) that demonstrated use the space according to interspecific preferences and presenting distribution patterns with the occurrence of distributed Juveniles throughout the year, suggesting that the studied environments are used by many species such as nursery areas and these have food preferences for zooplankton and mostly characterized as marine fish that frequent the estuary at some period of their life cycle. The species A. brasiliensis was constant in the spatial and temporal distribution, L. batesii was more abundant in the dry and the species L. grossidens, M. incilis, R. bahiensis, C. edentulus were dominant in the rainy season. The abiotic factors did not change much numerically, being only significant for dissolved oxygen between stations and different salinity for locations. We can see the meadows shown to be constant in the temporal variation and differed between sites, which showed no significant differences between the evaluation variables. Thus we can infer that the studied phytal environment is considered a nursery, it is frequented by several species of mostly juvenile fish that use this area as a place of recruitment, food and shelter; the dynamics of patches of seagrass were stable during the dry and rainy seasons and the composition and abundance of fish community is more explained by the spatial dynamics, but still have temporal distribution patterns, these features are typical of tropical environments that are inserted in mosaic adjacent environments. / Os prados de fanerógamas marinhas são importantes por serem áreas de berçário de muitas espécies de peixes. A fim de descrever a estrutura da comunidade, padrões temporais e espaciais de peixes que utilizam prados de Spartina alterniflora da Ilha do Maranhão, presentes em região de macromarés foram realizadas seis amostragens de nove pontos no período entre Julho de 2012 e junho de 2013, utilizando uma rede de arrasto. Um total de 11.660 indivíduos capturados, correspondendo a 80 espécies, na qual 13 são nova ocorrência para o estado do Maranhão. Os pontos amostrados são representados por algumas espécies abundantes (Rhinosardinia bahiensis, Atherinella brasiliensis, Lycengraulis grossidens, Lycengraulis batesii, Mugil incilis e Centegraulis edentulus) que demonstraram utilizar o espaço de acordo com preferências interespecíficas e apresentando padrões de distribuição com a ocorrência dos indivíduos juvenis distribuída durante todo o ano, sugerindo que os ambientes estudados são utilizados por várias espécies como áreas de berçário e estas apresentam preferências alimentar por zooplancton em sua maioria e caracterizadas como peixes marinhos que frequentam o estuário em algum período do seu ciclo de vida. A espécie A. brasiliensis mostrou-se constante na distribuição espacial e temporal, L. batesii foi mais abundante na seca e as espécies L. grossidens, M. incilis, R. bahiensis, C. edentulus foram dominantes no período chuvoso. Os fatores abióticos não variaram muito numericamente, apenas sendo significante para oxigênio dissolvido entre as estações e salinidade diferente para locais. Podemos observar os prados mostraram-se constantes na variação temporal e diferiu entre os locais, os quais não apresentaram variações significativas entre as variáveis de avaliação. Dessa maneira podemos inferir que o ambiente fital estudado é considerado um berçário, pois é frequentado por várias espécies de peixes principalmente juvenis que utilizam essa área como local de recrutamento, alimentação e refúgio; a dinâmica dos prados de fanerógamas se mantiveram estáveis durante os períodos seco e chuvoso e que a composição e abundância da comunidade de peixes é mais explicada pela dinâmica espacial, mas ainda assim possuem padrões de distribuição temporal, essas características são típicas de ambientes tropicais que estão inseridos em mosaico de ambientes adjacentes.

I carried out an observational study of historic high resolution aerial imagery spanning six decades (1950-2014) to identify recent and historic spatial extent of mangrove forests, within the municipal boundaries of the City of Tampa, Florida USA. My objectives were to map mangrove distribution and spatial extent and any change or patterns of change discernable. I observed variable patterns of change and rates of expansion varied between sites spatially as well as within sites between time intervals. I found notable changes in mangrove extent in the Tampa from historic and modern aerial imagery for the ~64-year period between 1950 and 2014. There were significant losses in areal extent between 1950 and 1973, much of which could be directly attributed to anthropogenic modification of the Tampa coastal landscape. All the regions observed had recovered or surpassed their original extent by the end of the period reviewed (1950-2014). It appears much of the recovery observed is a result of recolonization of created or modified shorelines. Results of these observations may contribute to the body of information used to inform conservation and management objectives in the City of Tampa and Tampa Bay.

Water quality characteristics of the heavily urbanised and industrialised Swartkops River catchment in the Eastern Cape has been the focus of several studies since the 1970s. Overloaded and poorly maintained wastewater treatment works (WWTW), polluted stormwater runoff and solid waste have had a negative impact on the water quality status of the Swartkops River and estuary. Past studies have revealed that a distinct relationship exists between land use activities and the water quality of the Swartkops Estuary, which in turn has raised concerns pertaining to the ecological, economic, recreational, and cultural value of the estuaryThe Swartkops Estuary has a Present Ecological State (PES) of Category D(1) and a Recommended Ecological Category (REC)(2) of a C (Van Niekerk et al., 2014). It is known that effective management of anthropogenic impacts on coastal systems requires a thorough understanding of the system’s biological responses to wastewater discharges and to hydrologic changes. For this reason, the objective of this study was to determine the current water quality status of the Swartkops Estuary, and to gain greater insight into factors controlling eutrophication. This was important as outdated water quality information was used in the Swartkops Integrated Environmental Management Plan (Enviro-Fish Africa, 2011) due to a lack of current data. To determine the current water quality status of the Swartkops Estuary the present study investigated spatial and temporal variability in physico-chemical parameters, nutrients, phytoplankton biomass and community composition, faecal bacteria, and “where possible” related this to historical water quality data. In general, points of entry into the estuary were investigated for their impact on nutrient enrichment and the bacteriological status of the estuary. Water quality surveys were completed in September 2012, November 2012, February 2013, May 2013 and August 2013The present study found evidence to suggest that water is not flushed as efficiently from the estuary as was previously the case, and that the natural hydrology of the estuary has been modified. These changes appear to be the effect of large volumes of wastewater discharges from the wastewater treatment works (WWTW), which has led to the additional stresses of increased vertical stratification and reduced vertical mixing. A build-up of dissolved inorganic nutrients has given rise to persistent eutrophic conditions and phytoplankton blooms occurring from the middle reaches to the tidal limit of the estuary. These findings were associated with a generally well oxygenated estuary; however, bottom water hypoxic conditions were recorded in the upper reaches of the estuary on two occasions and were generally associated with bloom.forming flagellates. Elevated concentrations of inorganic nutrients stimulated phytoplankton to attain high biomass ranging from 0 to 248 g l-1 (31.8 6.56 g l-1). All nutrients displayed positive linear gradients from the mouth to the tidal limit, showed significant (p < 0.05) temporal and spatial variability, and were significantly (p < 0.05) correlated with phytoplankton biomass. Phytoplankton blooms (> 10 000 cells ml-1) of several different groups were recorded from the middle reaches of the estuary to the tidal limit. Diatoms were the dominant group during increased freshwater inflow (at mean daily flow rate of 2.14 m3 s-1) and low DIP levels, whereas flagellates were generally the dominant group during reduced flow and under higher nutrient levels. Although the different tidal stages had no effect on phytoplankton biomass per se, it did support co-existance between phytoplankton groups. This was noted during the spring ebb tide in September 2012 (i.e. flagellates, diatoms and dinoflagellate) and in February 2013 (i.e. dinoflagellates, diatoms and chlorophytes). Phytoplankton blooms have become persistent in the middle to upper reaches of the estuary where chlorophyll-a was > 20 µg l-1 and cell density exceeded 10 000 cells ml-1; a situation not reported in previous studies. The Motherwell Canal was and still is the main source of nitrogen (generally in the form of ammonium) to the estuary, whereas the Swartkops River is still the primary source of phosphorus to the estuary. Since the stormwater canal services the large residential area of Motherwell where leaks in the sewer system, the dumping of night soil buckets, and faulty pumps are often reported, polluted discharges from the Motherwell Canal can enter the canal at any given point. In contrast to the canal, DIP loading from the Swartkops River to the estuary generally occurred under conditions of low flow, whereas nitrogen showed no apparent relationship. Faecal bacteria originating from the Motherwell Canal had the most profound effect on the bacteriological status in the middle reaches of the estuary, whereas the Swartkops River had an intermediate effect due to bacteria die-offs occurring between the point of release from the WWTW to the riverine reaches and the tidal limit of the estuary. Nevertheless, Escherichia coli and enterococci levels are still high, especially in the summer months rendering the estuary unsafe for recreation during this season. Historical data on trace metals in the water column were limited and thus observations from the present study could not be concluded with much confidence. However, preliminary data suggest that levels of copper, zinc, iron and cadmium have increased by at least 90% in the estuary, at the tidal limit of the estuary and in the Markman and Motherwell canals. High inputs of nutrients, trace metals and faecal bacteria to the estuary from land-use activities indicate the necessity for remedial actions with the main objective being to conserve and protect the estuary’s recreational, ecological and economic functions.

Macrozoobenthic community structure and composition was investigated along a subtidal-intertidal gradient in the Mngazana Estuary. Six transects were sampled between the spring high water mark (HWST) and the bottom of the river channel in the lower estuary. Fifteen replicate samples were collected along each transect using a Van Veen type grab (211 cm2 bite) during each of three sampling sessions. Samples were sieved through a 500 μm mesh bag and the invertebrates stored in bottles for further analysis in the laboratory. Additional grab samples were collected for sediment particle size analysis and organic matter. Physical variables measured at each transect included: salinity, temperature, dissolved oxygen, depth, pH, percentage mud, organic content and turbidity. Sediment compactness was measured at all intertidal transects and additional sediment samples were collected at mid shore and high shore transects for percentage water content analysis. A total of 104 species were recorded along the intertidal-subtidal gradient in the sampling area. Species richness was higher in the subtidal zone compared to the intertidal zone and polychaetes numerically dominated the macrozoobenthic community at most transects, during all three sessions. At high shore transects the community was characterised by having fewer species, consisting mostly of brachyurans, polychaetes and gastropods. Shannon diversity index (H’) was generally higher for subtidal transects (x¯ = 2.3; range: 2.8 to 1) than for intertidal transects (x¯ = 1.4; range: 2.2 to 0.6) indicating that the distribution of individuals among species in the intertidal zone experienced greater variability. Results for Hill’s numbers followed the same trend as Shannon diversity with subtidal communities mostly consisting of abundant species followed by very abundant species. Intertidal communities generally exhibited lower numbers of abundant and very abundant species. Sedimentary characteristics played a major role in structuring benthic communities in comparison to other physico-chemical variables. Organic content and mud content of the substrate were identified as important factors influencing community patterns observed along the subtidal-intertidal gradient. In addition, sediment compactness and water content of the substrate was found to influence intertidal community structure. Subtidal community structure possibly had a greater dependence on seasonal variations in abiotic and/or biotic factors. Cluster dendrograms used in conjunction with MDS ordination mapping revealed that macrozoobenthic communities were generally distinct between high shore intertidal transects iii and subtidal transects. Most species exhibited a broad spatial distribution along the subtidal-intertidal gradient with mid and high shore transects being the exception. Most species also exhibited marked shifts in abundance and this was especially noticeable at the transition between the subtidal and intertidal zone. Two polychaete species, Prionospio sexoculata and Capitella capitata, were very abundant species and featured amongst the most numerically dominant species collected during each sampling session.

An ecological reserve study by the Department of Water Affairs on the Great Brak Estuary stated that there was a need to determine how much nitrogen and phosphorus was flowing through the estuary as well as how effective the macroalgae were at removing N and P. The objective of this study was to investigate the physico-chemical characteristics in the estuary and the influence of these on the submerged macrophytes and macroalgae. A nutrient budget for the estuary was developed in order to quantify the contribution of the submerged macrophytes and macroalgae relative to other contributing sources. The Wolwedans Dam located 3 km upstream from the estuary has reduced the amount of freshwater flow to the estuary by as much as 56 percent. The estuary has been allocated 2 x 106 m3 per annum of freshwater (ecological reserve) that is used to breach the mouth once or twice a year in spring or summer. Even though this water has been made available it is not sufficient to flush the estuary. Reduced flushing has led to an accumulation of organic matter and degradation in the water quality. Physico-chemical measurements between September 2010 and July 2012 showed that dissolved oxygen values were generally below 6 mg l-1. The average NH4+ concentration in the estuary was 7 μM and increased with depth to 12 μM at 2 m depths. Concentrations >45 μM were found in February and April 2011 at the 5 m deep hole at 3.4 km upstream. Negative correlations between dissolved oxygen and NH4+ during November 2010, February 2011, April 2011 and July 2011 (r = -0.68; -0.67; -0.63; -0.96) indicated that remineralisation of organic matter had occurred. Soluble reactive phosphorus (SRP) followed a similar trend to the NH4+ and was generally below 1 μM in the water column for most months, and had peaks at 1.0 km and 3.4 km in the bottom water. The abundance of submerged macrophytes and macroalgae below the N2 bridge were mostly influenced by mouth state and river inflow. During the closed phase the dominant macroalga Cladophora glomerata had an area cover ranging from 3000 to 6000 m2 while Zostera capensis and Ruppia cirrhosa covered an area of 2000 to 3500 m2 and 1500 to 2900 m2, respectively. After an artificial breach in February 2011, water drained out of the estuary leaving the alga stranded on the marshes and as the flood tide entered the macroalga was once again redistributed in the lower reaches. The alga utilised the available nutrients in the water column and expanded its area cover from 35000 m2 in February 2011 to 64000 m2 in March 2011. However, after the floods in June 2011, Cladophora glomerata had been washed out of the system while the submerged macrophytes responded positively extending their area cover. By comparing the artificial breach with the natural breach, and the effect on the estuary, an important observation was highlighted. Increasing the current allocated ecological reserve, and using a larger volume of water to breach the mouth artificially, would result in better scouring of sediment and associated organic matter out of the estuary. This would enable better oxygenation of the water column, reduce remineralisation and minimise algal blooms.

The present study was undertaken in the temporarily open/closed Mngazi and permanently open Mngazana estuaries, located on the subtropical east coast of South Africa. The results from this research will assist decision makers in the freshwater management of these systems. Intertidal and subtidal benthic chlorophyll a concentrations, water column chlorophyll a, nutrients and several physico-chemical parameters were measured between June 2002 and November 2003. The objective of this study was to determine if the presence of freshwater in the estuaries had an effect on the microalgae of both estuaries. Five sites were sampled in the Mngazi Estuary and 14 sites were sampled in the Mngazana Estuary. The average water column chlorophyll a was significantly higher (p<0.05) in the Mngazana Estuary (surface 7.8 ± 0.7 μg.l-1, bottom 6.4 ± 0.7 μg.l-1) compared to the Mngazi Estuary (surface 4.9 ± 1.2 μg.l-1, bottom 7.3 ± 1.5 μg.l-1). There was no evidence of an REI (river-estuary interface) zone in areas where the water column chlorophyll a concentrations were high even during open mouth conditions in the Mngazi Estuary. The REI is that area where salinity is less than 10 ppt and is characterized by high water column productivity. Even though both systems received some freshwater during the summer periods, this was not enough to stimulate phytoplankton growth and nutrient availability seems to be the major factor limiting phytoplankton in these systems. Flagellates and diatoms were the dominant phytoplankton groups in both estuaries during the entire sampling session. The relative abundance of the different phytoplankton groups did not show differences between sites. The relative abundance of flagellates was in most cases greater than 60% and diatoms made up the remainder. The average benthic chlorophyll a was higher in the Mngazana Estuary (intertidal 24 ± 6 μg.g-1 subtidal 15.2 ± 3 μg.g-1) compared to the Mngazi Estuary (intertidal 15.3 ± 4.3 μg.g-1 subtidal 5.4 ± 1.6 μg.g-1). Regions with high benthic chlorophyll a concentrations had high sediment organic content. Sediment organic content was higher in the Mngazana Estuary (1 percent - 8 percent) compared to the Mngazi Estuary (4 percent – 6.8 percent). The sites situated on the Main Channel had on average significantly higher (p<0.05) benthic chlorophyll a biomass compared to Creek 1 and Creek 2 in the Mngazana Estuary. Peaks in benthic chlorophyll a concentrations occurred in the intertidal sediments in Creek 1 (50.4 ± 13.4 μg.g-1) and Creek 2 (57.4 ± 1.4 μg.g-1) in the Mngazana Estuary, the peaks occurred in winter during a period of low freshwater inflow into the estuary. Microphytobenthic biomass measured in the Mngazi Estuary is among the lowest values reported in the literature for temporarily open/closed estuaries. Statistical 4 analysis showed no significant difference between benthic chlorophyll a during the different mouth conditions and sampling sessions in the Mngazi Estuary. Microalgal responses in the Mngazana Estuary were similar to those observed in other permanently open marine dominated estuaries. In the temporarily open/closed Mngazi Estuary microalgal characteristics were different to that of other temporarily open/closed estuaries probably because the estuary was only sampled in the open and semi-closed state.

The Mngazana and Mngazi estuaries are situated just south of Port St. Johns close to the biogeographical boundary between the warm temperate and subtropical zones along the east coast of Southern Africa. The Mngazana estuary is a permanently open estuary with three species of mangrove. The Mngazi estuary is a temporarily open/closed system. The ichthyofauna was sampled with seine nets and gillnets during June 2005, August 2005, November 2005, February 2006 and April 2006. The fish community structure was significantly different between the two estuaries with the differences being especially noticeable in the larger fish sampled with gillnets. There were significant differences in the fish community sampled with the seine net on both spatial and temporal scales in both of the estuaries. Certain warm temperate species were less abundant and certain subtropical species were more abundant at the Mngazana estuary than they were during a study conducted in 1975. Stomach content analysis revealed that most species had similar diets in the two estuaries with the only differences occurring among benthic feeding species. The diet of sub-adult piscivores comprised of a large proportion of penaeid prawns while the juveniles feed mainly on mysids. The zooplanktivorous fish feed mainly on copepods and mysids with the proportions varying between the species. Benthic feeding fishes showed the greatest variability in diet with plant material, detritus, crabs and copepods being the main food sources. Stable isotopes revealed that particulate organic matter and microphytobenthos were the most important carbon sources to fish in the Mngazi estuary. In the Mngazana estuary a combination of mangrove material, detritus, particulate organic matter and Zostera material were the most important carbon sources. The importance of each of these sources did, however, vary greatly between the different trophic guilds of the fish in the Mngazana estuary. The distribution of certain fish species was investigated in relation to physical parameters (temperature, salinity, turbidity and depth) and biological parameters (prey and predators) in the environment. It was found that most small fish (< 100 mm SL) were strongly correlated with turbidity where-as they showed weak correlations with their prey. Sub-adult piscivores fell into two groups, i.e. those that could tolerate turbid water and those that were only found in clear water. The group that also occurred in turbid water showed very strong correlations with their prey but the clear water group did not. It would appear that the small and thus vulnerable fish seemed to occur in areas that offered them protection rather than areas where their food supply is most abundant, where-as the piscivores were more influenced by prey distribution, as long as the physical conditions were tolerable to them.