Studies were conducted at three different sites in Saskatchewan, Canada (Colonsay, Vanscoy and Rosthern) over two years (2005 and 2006) to determine the effect of dribble banded and coulter injected liquid fertilizer applied in the spring of 2005 at 56, 112 and 224 kg N ha-1 with and without P at 28 kg P2O5 ha-1. A similar study was conducted in 2006 at one site in Mongolia to determine the effect of granular N and P fertilizer application on dry matter yield, and N and P concentration in plant biomass in the year of application (2006) only. The three Saskatchewan sites were unfertilized, 7-8 year old stands of mainly meadow bromegrass (<i>Bromus riparius</i>) dominated haylands, while the Mongolia site was mixed species of native rangeland.<p>All fertilization treatments produced significantly (p≤0.05) higher dry matter yield than the control in the year of application at the three Saskatchewan sites. The addition of 28 kg P2O5 ha-1 P fertilizer along with the N fertilizer did not have a significant effect on yield in most cases. In the year of application, increasing N rates above 56 kg N ha-1 did not significantly increase yield over the 56 kg N ha-1 rate in most cases but did increase N concentration, N uptake and protein content. A significant residual effect was found in the high N rate treatments in 2006, with significantly higher yield and N uptake. In 2005, the forage N and P uptake were in all cases significantly higher than the control in the fertilized treatments. The N uptake at the three Saskatchewan sites increased with increasing N rate up to the high rate of 224 kg N ha-1, although the percent recovery decreased with increasing rate. The P fertilization with 28 kg P2O5 ha-1 also increased P uptake at the three Saskatchewan sites. The site in Mongolia was less responsive to fertilization than the three Saskatchewan sites, with only a significant response in yield, N uptake and no significant effect of P fertilization. <p>For incubation soil cores collected in the fall of 2006, mean respiration rates were similar among the fertilized treatments at all the sites and the pattern of CO2 and N2O evolution measured over a two-week period showed similar trend at the three sites, with no significant difference between treatments. However a significant increase in gas production occured as the soils were wetted during the incubation. By the fall of 2005, the fertilization effect had likely diminished along with available substrate for the soil microbial biomass.<p>Overall, rates of fertilizer of approximately 50 kg N ha-1 appear to be sufficient to produce nearly maximum yield and protein concentration of the grass in the year of application for the Saskatchewan and Mongolia sites. Surface banding placement was as effective as in soil placement and there was limited response to P fertilization. A small amount of N fertilizer that is surface-placed on these grass dominated forage systems appears to be an effective means of increasing production in the year of application. Higher rates are needed to sustain the rejuvenation beyond one year.

PC4/PCSK4 is the major Proprotein Convertase (PC) enzyme that plays a key role in mammalian fertilisation. It is detected in the acrosomal granules of round spermatids, acrosomal ridges of elongated spermatids and sperm plasma membrane overlying the acrosome with K-X-K/X-R as its preferred cleavage motif. Such motifs are present in male germ cell proteins ADAMs, proPACAP and proIGF-1/2 and these precursor proteins are processed most likely by PC4 during spermatogenesis, sperm maturation and sperm-egg interaction. For fertilization to occur, the mature sperm must penetrate the Zona Pelucida (ZP) and bind to the egg. Previously, PC4 null mouse sperm and wild type sperm treated with a specific PC4-inhibitor have shown to reduced abilities to penetrate the cumulus mass, bind to ZP and fertilize eggs. These findings suggest that sperm-PC4 plays an important role in fertilization and hence regulation of its activity is crucial for successful fertilization. But how PC4 activity is regulated in vivo is not yet clear. Recently, in epididymal fluid a serpin (serine protease inhibitor) called CRES has been described but the protease linked to this serpin in epididymis has not been identified. However in endocrine cells where CRES is also expressed, it inhibits PC2 enzyme. Thus based on localization and preliminary study, we propose that PC4 is the target enzyme for CRES in the reproductive tract. During sperm migration and storage in epididymis, sperm PC4 activity may be modulated by CRES so that premature sperm activation may not occur. Our data showed that CRES inhibits PC4 both in vitro (with IC50 in µM range) as well as ex vivo in human placenta trophoblast cell lines. Moreover CRES was found to be cleaved by PC4 suggesting a Serpin-Protease binding type of mechanism in the inhibition of protease activity. Taken together, we conclude that CRES regulates PC4 activity in reproductive tract crucial for mammalian fertilization.

Introducción: Desde su primera descripción en 1984 las indicaciones de donación de óvulos han ido aumentando, lo que ha provocado un incremento progresivo en el número de ciclos realizados a nivel mundial. Aunque esta técnica de reproducción garantiza una elevada tasa de embarazo en la receptora, los profesionales también se esfuerzan en convertir el tratamiento para la donante en un proceso sencillo y seguro. Durante los últimos diez años los antagonistas de la GnRH, por sus características farmacodinámicas, ha sido el fármaco utilizado para desarrollar protocolos de estimulaciones ováricas sencillos y seguros para las donantes de óvulos. Materiales: La presente tesis doctoral resume las conclusiones de dos artículos publicados recientemente (2010) sobre la utilización de antagonistas de GnRH en la estimulación ovárica de donantes de óvulos. Además se discuten las conclusiones de otros cuatro artículos (publicados en revistas científicas de impacto durante los años 2006 y 2009) estrechamente relacionados a los aquí publicados. Los objetivos de esta tesis son: 1. comparar la eficacia de protocolos de estimulación basados en antagonistas de GnRH en comparación con protocolos basados en agonistas de GnRH a través de una revisión sistemática de la literatura y meta-análisis. 2. ilustrar que los protocolos de estimulación basados en antagonistas de GnRH aumentan la seguridad de la estimulación ovárica para la donante a través de una eliminación completa del riesgo del síndrome de hiperestimulación ovárica (SHO). Resultados: 1. El meta-análisis de de ocho ensayos clínicos llevados al cabo en donantes de óvulos estimuladas con antagonistas de GnRH no han demostrado diferencia significativa en el número de ovocitos obtenidos y las tasas de embarazo evolutivos en las receptoras correspondientes en comparación con agonistas de GnRH. 2. El estudio observacional, prospectivo realizado en donantes de óvulos de alto riesgo ha demostrado la eliminación completa de SHO moderado/severo tras la descarga con bolo de agonista de GnRH. Además se discuten las conclusiones de cuatro otros estudios apoyando las conclusiones mencionados arriba. Conclusiones: En el contexto de la donación de óvulos los protocolos de estimulación ovárica basados en antagonistas de la GnRH son igual de eficaces que los protocolos con agonistas de la GnRH. La inducción final de la maduración ovocitaria se puede llevar a cabo satisfactoriamente con un bolo de agonista de GnRH en vez de hCG, lo que prácticamente elimina el riesgo de SHO moderado/severo. La utilización preferencial de este protocolo de estimulación ovárica es muy aconsejable porque permite un tratamiento más sencillo y aumenta considerablemente la seguridad de la estimulación ovárica en donantes de óvulos. / Background: Since its first description in 1984 the indications of oocyte donation (OD) has widened considerably which has led to a continuous increase in the number of OD treatment cycles performed worldwide. Although this treatment option secured the highest pregnancy rates for the recipients of donor oocytes increased efforts were also made to achieve safer and simpler treament protocols for the oocyte donor. During the last decade with the advent and increased use of the GnRH antagonists this new pharmacological agent was also explored in ovarian stimulation protocols specifically tailored for oocyte donors. Materials: The present doctoral thesis summarizes the findings of two recently published articles (2010) on the application of GnRH antagonists in the ovarian stimulation of oocyte donors. Furthermore the findings of another four strictly related articles (published in high-impact international journals between 2006 and 2009) are also discussed. The primary objectives were: 1. to compare efficiency of GnRH antagonist protocols in comparison with GnRH agonist-based protocols in the context of oocyte donation by means of a systematic review and meta-analysis and 2. to illustrate that GnRH antagonist protocols substantially increase the safety of ovarian stimulation for oocyte donors by reducing or even eliminating the incidence of moderate/severe ovarian hyperstimulation syndrome (OHSS). Results: 1. A meta-analysis of eight randomized clinical trials (RCTs) performed in oocyte donors undergoing stimulation with GnRH antagonists showed no significant difference in the number of retrieved oocytes or recipient ongoing pregnancy rate when compared with GnRH agonists. 2. A prospective, follow-up study of a group of high risk oocyte donors showed that early onset moderate/severe OHSS was completely eliminated after triggering with a GnRH agonist. Furthermore the findings of four studies supporting the above conclusions are also presented. Conclusions: In the context of oocyte donation the GnRH antagonist based ovarian stimulation protocols are equally efficient compared to down regulation by GnRH agonists. The induction of final oocyte maturation can be successfully achieved by a GnRH agonist instead of hCG which practically eliminates early-onset moderate/severe OHSS. The proposed ovarian stimulation protocol should be preferentially used because it permits the simplification and considerably increases the safety of ovarian stimulation for oocyte donors.

Für unterschiedliche Standorte wurden Humusbilanzen für einzelne Fruchtarten und Fruchtfolgen berechnet. Betrachtet wurden reine Marktfruchtbetriebe mit Anbau von Körnerfrüchten (Getreide, Raps, Körnermais) ohne Viehhaltung, Betriebe mit unterschiedlich intensiver Schweinehaltung, Marktfruchtbetriebe mit steigender Anbaukonzentration verschiedener Hackfrüchte und Betriebe mit Anbau von Silomais in Energiefruchtfolgen mit Rinder- und Milchviehhaltung.

Meadow bromegrass (<i>Bromus riparius</i> Rehm.) is an important forage grass in western Canada. Economical seed production is critical to its use. Seed yield usually declines rapidly after two to three seed crops. Field and growth chamber experiments were conducted to determine the influence of (a) residue removal and N fertilization on tiller density and size, panicle density, silvertop incidence, seed yield and stand longevity, (b) filler size and stand age on panicle production, and (c) daylength and temperature during primary and secondary induction on panicle production. Removing residue after harvest and applying N (100 kg ha-1 ) increased yield from 200 to 450 kg ha-1 compared to not removing residue or adding N in the second seed crop. The difference between treated and untreated plots was only 30 to 90 kg ha-1 in the third seed crop. The increase was related to an increase in panicle production, however, the correlation between panicle number and seed yield was low. Silvertop incidence (% of panicles affected) increased as the stand aged, but removing residue after harvest reduced silvertop. In pot studies, the percentage of plants that produced panicles increased as tiller basal diameter increased from one mm to three mm, regardless of the age of the stand. However, fewer large tillers were observed in older stands. Large tillers from a four-yr-old stand produced fewer particles than large tillers from a two-yr-old stand indicating that tiller size alone is not responsible for the decline in panicle production. Panicle production increased as the temperature during primary induction decreased. However, daylength during primary induction had no effect on panicle production. Varying temperature or daylength during secondary induction had no effect on panicle production; panicles were produced in 85% of plants regardless of temperature, and 67 to 77% of the plants regardless of daylength. In conclusion, residue removal after harvest and N fertilization improve seed yield in young meadow bromegrass stands. However, these practices were not effective in prolonging seed yield beyond two to three seed crops. Drought, winter injury, competition amongst tillers and silvertop incidence all play a role in reducing seed production.

Studies were conducted at three different sites in Saskatchewan, Canada (Colonsay, Vanscoy and Rosthern) over two years (2005 and 2006) to determine the effect of dribble banded and coulter injected liquid fertilizer applied in the spring of 2005 at 56, 112 and 224 kg N ha-1 with and without P at 28 kg P2O5 ha-1. A similar study was conducted in 2006 at one site in Mongolia to determine the effect of granular N and P fertilizer application on dry matter yield, and N and P concentration in plant biomass in the year of application (2006) only. The three Saskatchewan sites were unfertilized, 7-8 year old stands of mainly meadow bromegrass (<i>Bromus riparius</i>) dominated haylands, while the Mongolia site was mixed species of native rangeland.<p>All fertilization treatments produced significantly (p≤0.05) higher dry matter yield than the control in the year of application at the three Saskatchewan sites. The addition of 28 kg P2O5 ha-1 P fertilizer along with the N fertilizer did not have a significant effect on yield in most cases. In the year of application, increasing N rates above 56 kg N ha-1 did not significantly increase yield over the 56 kg N ha-1 rate in most cases but did increase N concentration, N uptake and protein content. A significant residual effect was found in the high N rate treatments in 2006, with significantly higher yield and N uptake. In 2005, the forage N and P uptake were in all cases significantly higher than the control in the fertilized treatments. The N uptake at the three Saskatchewan sites increased with increasing N rate up to the high rate of 224 kg N ha-1, although the percent recovery decreased with increasing rate. The P fertilization with 28 kg P2O5 ha-1 also increased P uptake at the three Saskatchewan sites. The site in Mongolia was less responsive to fertilization than the three Saskatchewan sites, with only a significant response in yield, N uptake and no significant effect of P fertilization. <p>For incubation soil cores collected in the fall of 2006, mean respiration rates were similar among the fertilized treatments at all the sites and the pattern of CO2 and N2O evolution measured over a two-week period showed similar trend at the three sites, with no significant difference between treatments. However a significant increase in gas production occured as the soils were wetted during the incubation. By the fall of 2005, the fertilization effect had likely diminished along with available substrate for the soil microbial biomass.<p>Overall, rates of fertilizer of approximately 50 kg N ha-1 appear to be sufficient to produce nearly maximum yield and protein concentration of the grass in the year of application for the Saskatchewan and Mongolia sites. Surface banding placement was as effective as in soil placement and there was limited response to P fertilization. A small amount of N fertilizer that is surface-placed on these grass dominated forage systems appears to be an effective means of increasing production in the year of application. Higher rates are needed to sustain the rejuvenation beyond one year.

Increased development in Canadas northern environments has increased the need for accurate methods to detect adverse impacts on tundra ecosystems. Ammonium nitrate is a common water pollutant associated with many industrial and municipal activities, including diamond mining, and is of special concern due to the toxicity of ammonia in aquatic systems. One solution to reduce exposure of sensitive aquatic systems to nitrogenous compounds is to atomize (atmospherically disperse in fine particles) contaminated water over the arctic tundra which will reduce N loading to surface water. However, the toxicity of ammonium nitrate to arctic soils is poorly understood. In this study I investigate the potential toxicity of ammonium nitrate solutions to arctic soil functions such as carbon mineralization, nitrification and plant growth, to determine concentrations that can be applied without causing significant inhibition to these processes. <p>Arctic ecosystems are based on a soil type termed a cryosol that has an underlying permafrost layer. Often these soils are subject to cryoturbation, a process which heaves and mixes the soil, bringing the mineral horizons to the surface. I hypothesized that phytotoxicity test results in arctic soils would be highly variable compared to other terrestrial ecosystems due to the cryoturbation process and subsequent range of soil characteristics. The variability associated with phytotoxicity tests was evaluated using Environment Canadas standardized plant toxicity test in three cryoturbated soils from Canadas arctic exposed to a reference toxicant, boric acid. The phytotoxicity of boric acid to northern wheatgrass (<i>Elymus lanceolatus </i>) in cryosols was much greater than commonly reported in other soils, with less than 150 ug boric acid g-1 soil needed to inhibit root and shoot growth by 20%. There was also large variability in the phytotoxicity test results, with coefficients of variation for 10 samples ranging from 160 to 79%. Due to this variability in cryoturbated arctic soils, more than 30 samples should be collected from each control and potentially impacted area to accurately assess contaminant effects, and ensure that false negatives of toxicant impacts in arctic soils are minimized. <p>To characterize the toxicity of ammonium nitrate I exposed a variety of arctic soils and a temperate soil to different concentrations of ammonium nitrate solution over a 90 day time period. Dose responses of carbon mineralization, nitrification and phytotoxicity test parameters were estimated for ammonium nitrate applications. In addition to direct toxicity, the effect of ammonium nitrate on ecosystem resistance was investigated by dosing nitrogen impacted soils with boric acid. Ammonium nitrate solutions had no effect on carbon mineralization activity, and affected nitrification rates in only one soil, a polar desert soil from Cornwallis Island. In contrast, ammonium nitrate applications (43 mmol N L-1 soil water) significantly impaired seedling emergence, root length and shoot length of northern wheatgrass. Concentrations of ammonium nitrate in soil water that inhibited plant parameters by 20% varied between 43 to 280 mmol N L-1 soil water, which corresponds with 2,100 to 15,801 mg L-1 in the application water. Arctic soils were more resistant to ammonium nitrate toxicity than the temperate soil under these study conditions. However, it is not clear if this represents a general trend for all polar soils, and because nitrogen is an essential macro-nutrient, nitrogenous toxicity should likely be considered a special case for soil toxicity. As soil concentrations could be maintained under inhibitory levels with continual application of low concentrations of ammonium nitrate over the growing season, atomization of wastewater contaminated with ammonium nitrate is a promising technology for mitigation of nitrogen pollution in polar environments. <p>Increased development in Canadas northern environments has increased the need for accurate methods to detect adverse impacts on tundra ecosystems. Ammonium nitrate is a common water pollutant associated with many industrial and municipal activities, including diamond mining, and is of special concern due to the toxicity of ammonia in aquatic systems. One solution to reduce exposure of sensitive aquatic systems to nitrogenous compounds is to atomize (atmospherically disperse in fine particles) contaminated water over the arctic tundra which will reduce N loading to surface water. However, the toxicity of ammonium nitrate to arctic soils is poorly understood. In this study I investigate the potential toxicity of ammonium nitrate solutions to arctic soil functions such as carbon mineralization, nitrification and plant growth, to determine concentrations that can be applied without causing significant inhibition to these processes. <p>Arctic ecosystems are based on a soil type termed a cryosol that has an underlying permafrost layer. Often these soils are subject to cryoturbation, a process which heaves and mixes the soil, bringing the mineral horizons to the surface. I hypothesized that phytotoxicity test results in arctic soils would be highly variable compared to other terrestrial ecosystems due to the cryoturbation process and subsequent range of soil characteristics. The variability associated with phytotoxicity tests was evaluated using Environment Canadas standardized plant toxicity test in three cryoturbated soils from Canadas arctic exposed to a reference toxicant, boric acid. The phytotoxicity of boric acid to northern wheatgrass (<i>Elymus lanceolatus </i>) in cryosols was much greater than commonly reported in other soils, with less than 150 ug boric acid g-1 soil needed to inhibit root and shoot growth by 20%. There was also large variability in the phytotoxicity test results, with coefficients of variation for 10 samples ranging from 160 to 79%. Due to this variability in cryoturbated arctic soils, more than 30 samples should be collected from each control and potentially impacted area to accurately assess contaminant effects, and ensure that false negatives of toxicant impacts in arctic soils are minimized. <p>To characterize the toxicity of ammonium nitrate I exposed a variety of arctic soils and a temperate soil to different concentrations of ammonium nitrate solution over a 90 day time period. Dose responses of carbon mineralization, nitrification and phytotoxicity test parameters were estimated for ammonium nitrate applications. In addition to direct toxicity, the effect of ammonium nitrate on ecosystem resistance was investigated by dosing nitrogen impacted soils with boric acid. Ammonium nitrate solutions had no effect on carbon mineralization activity, and affected nitrification rates in only one soil, a polar desert soil from Cornwallis Island. In contrast, ammonium nitrate applications (43 mmol N L-1 soil water) significantly impaired seedling emergence, root length and shoot length of northern wheatgrass. Concentrations of ammonium nitrate in soil water that inhibited plant parameters by 20% varied between 43 to 280 mmol N L-1 soil water, which corresponds with 2,100 to 15,801 mg L-1 in the application water. Arctic soils were more resistant to ammonium nitrate toxicity than the temperate soil under these study conditions. However, it is not clear if this represents a general trend for all polar soils, and because nitrogen is an essential macro-nutrient, nitrogenous toxicity should likely be considered a special case for soil toxicity. As soil concentrations could be maintained under inhibitory levels with continual application of low concentrations of ammonium nitrate over the growing season, atomization of wastewater contaminated with ammonium nitrate is a promising technology for mitigation of nitrogen pollution in polar environments.

An Zierpflanzen im Gewächshaus wurden mengenbilanzierte Düngungsverfahren getestet. Die Düngegaben erfolgten in Wochenportionen per Hand, mit Dosiergeräten, per Düngecomputer oder als Depotdünger. In die Versuche wurden Pelargonien, Sommertopfkulturen im Gewächshaus, Poinsettien und Cyclamen einbezogen. Wochenportionen können nur bei einheitlichen Anbausätzen je Bewässerungseinheit realisiert werden. Der Kostenaufwand bei Depotdünger beträgt das Dreifache gegenüber einer Bewässerungsdüngung. Allerdings sind die Düngeverluste geringer. Mit einer mengenbilanzierten Düngung lassen sich Über- und Unterversorgung der Pflanzen vermeiden.

Die Genauigkeit und Eignung verschiedener Methoden zur Humusbilanzierung werden für den Einsatz in der konventionellen und ökologischen Landwirtschaft ausgewertet. Weiterhin wurde das Versorgungsniveau mit organischer Substanz auf den Dauertestflächen sächsischer Betriebe beurteilt. Anhand der genaueren, standortangepassten Methode zur Humusbilanzierung zeigt sich, dass in Sachsen die Hälfte der Ackerflächen mit organischer Substanz optimal versorgt ist. Knapp ein Drittel der Schläge erhält eine hohe bis sehr hohe Versorgung, der Anteil unterversorgter Flächen liegt bei 20 Prozent.

Nutrition of host plants has been shown to have a direct effect on the productivity of numerous insect pests, including western flower thrips [(WFT) Frankliniella occidentalis (Pergande)] – a major pest on both horticulture and agronomic crops. Plants use constitutive and induced chemical defenses to aid in protection against phytophagous insects. Reductions in WFT abundance in response to decreased nutrient availability has been attributed to the reduced availability of nutrients required for WFT productivity. The goals of this research were to determine the effects of fertilization on chemical defenses, and subsequent effects on WFT feeding and abundance. More importantly, the effects of fertilization and WFT feeding on plant growth, development, physiology, and quality were determined to assess the viability of optimizing fertilization in order to increase host plant resistance in gerbera. Constitutive (i.e. phenolics) and induced (i.e. jasmonic acid) chemical defenses were enhanced when fertilization was reduced. Reducing fertilization increased the total phenolics and wound- and WFT-induced jasmonic acid (JA) accumulation in gerbera. The enhanced chemical defenses in lower fertility plants resulted in reduced WFT abundance and feeding damage. These results indicate that the strategy for some plant species under nutrient stress is to increase constitutive defenses, while maintaining, or possibly increasing inducible defenses instead of growth. Similar to 0X fertility plants (only supplied with initial fertilizer charge in commercial media), 0.3X (received 30% of recommended rate) gerberas had reduced biomass and greater chemical defenses compared to 1X plants, but these plants did not appear to be nutritionally stressed—and 0.3X plants without WFT were rated as marketable. Reducing fertilization by 70% (0.3X) did not affect flower dry mass (DM) or the rate of flowering, but the flower stalks (peduncles) were taller in response to the fertilizer reduction. Hence, reducing fertilization to a moderate level in gerbera production may reduce susceptibility to WFT, while producing marketable crops.