The subject of the thesis Evaluation criteria for the evaluation of directors of houses for children and youth is an analysis of the current status of the evaluation process directors by founders of houses for children and youth (DDM). The aim of this thesis is to determine how the assessment is carried out DDM founder of directors, including the sources, methods and criteria that are used in the evaluation. My fundamental goal by this thesis: To determine whether the results of the evaluation are used in the further development directors DDM and whether the directors and founders believe that the evaluation of the founder directors is necessary for improving the quality of DDM. The theoretical part deals with the definition houses for children and youth, director DDM, founder DDM, staff evaluation and training and development. In the practical part of the research - descriptive type of research, conducted through questionnaires on the basis of three hypotheses. Conclusion the paper provides an overview of the current condition.

TITLE: Specifics of the Church grammar schools in the Czech Republic AUTHOR: Bc. Jan Pech DEPARTMENT: School Management Center SUPERVISOR: Mgr. et. Bc. Jiří Trunda ABSTRACT: The churches and religious denominations can become school and school facility founders. What are the specifics of the church schools from the management of education point of view, which this work deals with? And do these specifics actually exist? Do the tangible differences that clearly specify the church school against the school founded by the municipality, region or the union of municipalities exist? We will focus on the church grammar schools. There are twenty such grammar schools in the Czech Republic and almost all of them, were founded by the Roman Catholic Church. These institutions create a specific, not large group of schools that in addition to efforts to provide high-quality education strive for transfer of religious values. The work looks into the historical development of the church schools and describes their actual position in the regional education system. Then, using the qualitative research method - the analysis of the materials, it deals with the issues of the church schools management and capturing their specifics. KEYWORDS: church school, church grammar school, financing of the church schools, founder of the...

This thesis deals with the application of approaches in current practice that consider the performance of the Deputy Director as an appropriate or necessary precondition for the future performance of the school principal or the school facility. This work investigates current approaches that are applied in practice by founders, directors and deputy directors of schools and school facilities. The work is based on the study of available literature and applicable laws in the Czech Republic. At the same time, it draws on international approaches currently applied in the field. The research was conducted through questionnaires and investigations of implementations in the selection of future directors. In addition, respondents' views of founders and directors of the inclusion conditions of the Deputy Director of the insolvency proceedings. Also, respondents' views on the possible acquisition of management skills by the acting deputy director were examined. The analysis of the research describes the state determined and recommends possible solutions for the questions in the next period with emphasis on the potential increase in the quality of the Czech education system.

Made available in DSpace on 2016-08-10T10:38:49Z (GMT). No. of bitstreams: 1 LUCIANA MOREIRA BORGES.pdf: 1848721 bytes, checksum: 3b9777e8d534e26ba687aa1328ac6555 (MD5) Previous issue date: 2014-04-07 / Introduction: the R337H mutation of the TP53 gene was originated in the Brazilian population through a founder effect and is considered a molecular defect of low penetrance. In combination with some polymorphisms, the R337H mutation can increase the susceptibility to tumor. The frequency of the R337H mutation in Brazilian families is considered high when compared to the observed frequencies in other countries. The unambiguous association between the mutation, the emergence of different tumor types and the high number of individual that carry the mutation makes the R337H a relevant factor in public health, particularly in the prediction of cancer. Objective: This study aimed to investigate the predictive value of the R337H mutation of the TP53 gene as a clinical marker in cancer patients. Method: a systematic literature review (SLR) was carefully performed, by searching electronic scientific literature in LILACS, IBECS, MEDLINE, Pubmed and SciELO. Twelve articles, published in English between the years 2006 to 2013, were selected by performed the relevance tests I and II. Extraction of detailed data was independently performed by two investigators, following on extraction data protocol. Results: the R337H mutation was found in 287 of 1,548 patients with cancer, two of 750 women considered healthy, 200 of 887 family members of patients with adrenocortical tumor (ACT) carrying the R337H mutation, 12 of 647 health controls and in 442 of 171 630 newborns. Eight of the twelve selected references associated the R337H mutation with family history of 411 patients with the mutation. Four studies associated the R337H mutation prognosis. Conclusion: the frequency of the R337H mutation of the TP53 gene is considerably higher in the south and southeast regions of Brazil compared to other countries. The mutation was associated with family history of cancer, the increase of the positive predictive value and the decreased of negative predictive value at diagnosis, and poor prognosis in ACT and CPC patients with with the mutation. / Introdução: a mutação R337H do gene TP53 foi originada na população brasileira por efeito fundador e é considerada um defeito molecular de baixa penetrância. Em combinação com alguns polimorfismos, a mutação R337H, pode aumentar a susceptibilidade ao desenvolvimento do tumor. A frequência da mutação R337H em famílias brasileiras é considerada elevada, quando comparada com as frequências observadas em outros países. A inequívoca associação entre a mutação, o surgimento de diferentes tipos tumorais e o alto número de indivíduos portadores da mutação fazem da R337H um relevante fator de saúde pública, em especial na predição do câncer. Objetivos: este estudo objetiva de investiga o valor preditivo da mutação R337H do gene TP53 como um marcador clínico em pacientes com câncer. Método: revisão bibliográfica sistemática (RBS) criteriosa foi realizada, através de busca eletrônica de artigos científicos nas bases de dados LILACS, IBECS, MEDLINE, Scielo e Pubmed. Doze artigos selecionados foram publicados em língua inglesa entre os anos de 2006 à 2013, foram selecionados para aplicação dos testes de relevância I e II. Extração de dados detalhada foi realizada de forma independente por dois investigadores seguindo o protocolo para extração de dados. Resultados: a mutação R337H foi encontrada em 287 dos 1.548 portadores de câncer, duas das 750 mulheres consideradas saudáveis, 200 dos 887 familiares de pacientes portadores de tumor do córtex adrenal (TCA) com a mutação R337H, doze dos 647 controles e 442 dos 171.630 recém-nascidos. Oito das doze referências selecionadas associaram a mutação R337H com histórico familiar de 411 pacientes com a mutação. Quatro estudos associaram a mutação R337H com o prognóstico. Conclusão: a frequência da mutação R337H do gene TP53 é consideravelmente mais elevada no sul e sudeste do Brasil quando comparada com os demais países do mundo. A mutação foi associada com: histórico familiar de câncer, aumento do valor preditivo positivo e diminuição do valor preditivo negativo no diagnóstico e mal prognóstico em pacientes com ACT e CPC com a mutação.

A incidência dos tumores adrenocorticais na região sul do Brasil é 10-15 vezes maior que a incidência mundial. Mutações no gene supressor tumoral p53, localizado na região 17p13.1 têm sido identificadas em diversos tumores humanos. Uma distinta mutação germinativa, Arg337His, localizada no domínio de tetramerização da proteína supressora tumoral P53 foi identificada em 35 de 36 crianças da região sul do Brasil. No presente trabalho, investigamos a presença da mutação Arg337His em 71 pacientes não relacionados, 41 adultos e 30 crianças, portadores de tumores adrenocorticais benignos e malignos. Adicionalmente, análise de perda de heterozigose do locus p53, mapa de deleção do cromossomo 17 e instabilidade cromossômica foram estudados em DNA genômico destes pacientes. Nenhum dos pacientes estudados apresentava histórico familial compatível com a síndrome de Li-Fraumeni. Sequenciamento automático permitiu a identificação da mutação Arg337His, em DNA extraído a partir de sangue periférico e/ou tecido tumoral, em 29 (24 crianças e 5 adultos) dos 71 pacientes. Nas 10 famílias em que foi possível analisar o DNA genômico de ambos os pais verificamos que a mutação Arg337His tem caráter hereditário. Por outro lado, esta mutação não foi encontrada em DNA de 160 indivíduos do grupo controle, não relacionados, analisados por sequenciamento automático e/ou digestão enzimática. A análise pareada de DNA gênomico de sangue periférico e de tecido tumoral revelou perda de heterozigose para o locus p53 em 18 de 21 (86%) pacientes portadores da mutação Arg337His. Não observamos correlação entre a presença desta mutação e o comportamento maligno dos tumores. O estudo de dois marcadores polimórficos intragênicos do p53, pelo programa de análise de tamanho de fragmento GeneScan, evidenciou um mesmo haplótipo associado à mutação Arg337His em 91% dos pacientes com tumores adrenocorticais, configurando uma origem comum para esta mutação. O estudo de 6 marcadores polimórficos ao longo do cromossomo 17 (D17S926, VNTRP53, D17S1856, D17S942, D17S1351 e D17S928) em DNA genômico pareado de 29 pacientes demonstrou uma freqüência elevada (81%) de perda do cromossomo 17 em associação à mutação Arg337His. Não observamos correlação entre a perda do cromossomo 17 e a agressividade tumoral nestes pacientes. Instabilidade cromossômica envolvendo os cromossomos 2, 9 e 11 nos 17 pacientes que perderam o cromossomo 17 foi identificada em 47%, 47% e 71%, respectivamente. Perda dos cromossomos 2 e 11 foi evidenciada em tumores benignos e malignos. A perda do cromossomo 9 foi evidenciada exclusivamente nos tumores malignos, assim como a perda concomitante de 3 ou mais cromossomos. Em conclusão, confirmamos uma freqüência elevada da mutação Arg337His em crianças brasileiras com tumores adrenocorticais benignos e malignos. Esta mutação também foi encontrada no grupo de adultos, embora em menor freqüência. Não houve correlação entre sua presença e o comportamento maligno dos tumores adrenocorticais. Efeito fundador para a mutação Arg337His e inativação bialélica do p53, caracterizada pela presença da mutação Arg337His e a perda do cromossomo 17 foram demonstradas na maioria dos casos analisados. Finalmente, a instabilidade cromossômica envolvendo três ou mais cromossomos contribuiu para o diagnóstico de carcinoma adrenocortical / The incidence of adrenocortical tumors in the South region of Brazil is 10 to 15 times higher than the worldwide one. Mutations in the tumor suppressor p53 gene, located in chromosome 17p13.1, have been described in different human tumors. A germline mutation, Arg337His, in the tetramerization domain of the tumor suppressor P53 was identified in 35 of 36 children from the South region of Brazil. In the present study we have searched for Arg337His mutation in genomic DNA of 71 non-related patients, 41 adults and 30 children, with benign or malignant adrenocortical tumors. Additionally, we also analyzed the loss of heterozigosity of p53 locus, deletion mapping of chromosome 17 and chromosome instability, in genomic DNA of these patients. None of the patients had a familial history of Li-Fraumeni syndrome. Automatic sequencing identified the Arg337His mutation in genomic DNA from peripheral leukocytes and/or tumor tissues in 29 (24 children and 5 adults) of these 71 patients. In 10 families in which the study of both parent\'s DNA was possible, the Arg337His mutation was inherited from one of the parents. Sequencing analysis and/or enzymatic restriction showed that this mutation was not present in DNA of 160 non-related control subjects. Paired analysis of genomic DNA of peripheral leukocytes and tumor tissue revealed loss of heterozigosity of p53 locus in 18/21 (86%) patients with Arg337His mutation. There was no correlation between the presence of this mutation and the malignant behavior of these tumors. The study of two intragenic polymorphic markers of p53 through GeneScan software showed the association of the same haplotype with the Arg337His mutation in 91% of patients with adrenocortical tumors, indicating a common origin of this mutation. The study of 6 polymorphic markers along chromosome 17 (D17S926, VNTRP53, D17S1856, D17S942, D17S1351, D17S928) in paired genomic DNA of 29 patients showed an increased frequency (81%) of chromosome 17 loss in association with the presence of the Arg337His mutation. We did not observe any correlation between the loss of chromosome 17 and aggressive tumor behavior in these patients. In the 17 patients who lost chromosome 17, chromosome instability of chromosomes 2, 9 and 11 was identified in 47%, 47% e 71%, respectively. Loss of chromosomes 2 and 11 was observed in benign and malignant tumors, whereas the loss of chromosome 9 was observed exclusively on malignant tumors. Similarly, the concomitant loss of 3 or more chromosomes was only observed in malignant tumors. In conclusion we confirmed an increased frequency of Arg337His mutation in Brazilian children with benign or malignant adrenocortical tumors. This mutation was also found in the adult group, although at a lower frequency. There was no correlation between the presence of the mutation and the malignant behavior of adrenocortical tumor. We demonstrated a founder effect for this mutation and also a biallelic inactivation of p53 characterized by the presence of the Arg337His mutation and the loss of chromosome 17 in most of the cases studied. Finally, chromosome instability involving 3 or more chromosomes contributed for the diagnosis of adrenocortical carcinoma in these

The influence of the founders of public primary schools in small municipalities on the educational direction of the school Author: Bc. et Bc. Vojtěch Bílý ABSTRACT The thesis deals with the possible influence of founders of public primary school on the educational direction of the school. Although the law does not allow the direct influence of the founders on the management of the pedagogical process, there are a number of instruments by which the founders indirectly seek to apply their influence. In this work we first define the field of research, the state of the current knowledge of the subject and the basic concepts, and then we analyze the ways in which the founders use the influence, what pressure the school heads perceive and to what extent they are subject to it. In the practical part, we focus on primary schools in the Olomouc region in municipalities up to 5000 inhabitants where there is a prerequisite for the existence of only one school and there is usually a closer link between the school head and the mayor of the municipality. The practical part is based on questionnaires with the founders and the directors of the schools which are complemented with semi-structured interviews with the directors and the mayors of the municipalities. The results show that the majority of the founders do not...

Gaps exist in the literature on knowledge of how founder behavior affects volunteers and employees in nonprofit organizations. Through exploration of founder relationships with volunteers and employees, this study fills some of those gaps and adds to the body of knowledge of how those relationships are perceived by founders, volunteers, and employees. The purpose of this narrative study was to address the question of the impact of founder behavior on founders, employees and volunteers in nonprofit women's organizations founded by African American and Caucasian women. The theory of psychological ownership was used as the framework to understand founder behavior. The qualitative narrative inquiry design consisted of interviews with 12 participants who work for nonprofit organizations that provide empowerment services to women. Themes such as control, lack of strategy and support, and silenced voices emerged as responses to the question of the impact of founder behavior on the organization. The results indicate that founders, employees, and volunteers report a need for clear policies, role assignments, procedures, and organizational goals. Founders are oblivious to the impact of their behavior on the organization and are not aware of the available resources that may exist in their communities. Implications include state level nonprofit policy that funds training for nonprofit organizations around the themes examined in this study. Recommendations for future research include examination of internal issues and structures related to an organization's growth. The result of this study may lead to increased understanding of perceptions of the operations of nonprofit organizations which may impact nonprofit organizations' abilities to meet the goals of their mission.

<p>In this thesis, I examined variation in sex expression and mating patterns in the sexually polymorphic, wind-pollinated herb <i>Plantago maritima</i>. With a combination of field studies, greenhouse experiments, and genetic analyses, I (a) examined factors influencing sex ratio variation in gynodioecious plants (in which hermaphrodites and females coexist), (b) discovered variation in breeding system, (c) investigated density-dependence of seed production, and (d) documented genetic variation within and among populations close to the northern range margin in Europe. </p><p>In a survey of 104 <i>P. maritima</i> populations, I documented considerable variation in sex ratio (range 0-70% females, median 6.3% females). As predicted, females were more frequently missing from small than from large populations, and the variance in sex ratio increased with decreasing population size. Among twelve populations sampled for seed production, the frequency of females was positively related to relative fecundity of females and negatively related to population size. The results suggest that the local sex ratio is influenced both by the relative fecundity of females and hermaphrodites, and by stochastic processes in small populations.</p><p>A comparative field study showed that plant fecundity decreased with increasing distance to nearest pollen donor both within and among populations in an archipelago in southern Sweden, where self-incompatibility was confirmed in controlled crosses. In contrast, plant fecundity was overall higher and was not density-dependent in the Skeppsvik archipelago in northern Sweden, where controlled crosses showed that plants are self-compatible. The results were consistent with the prediction that evolution of self-fertility should reduce density-dependence of pollination success.</p><p>I quantified the genetic structure within and among populations from eastern Sweden and western Finland based on variation at four polymorphic microsatellite loci. The genetic diversity was low in northern Sweden, which may be the result of a history of small population sizes and periods of frequent self-fertilization.</p>

In this thesis, I examined variation in sex expression and mating patterns in the sexually polymorphic, wind-pollinated herb Plantago maritima. With a combination of field studies, greenhouse experiments, and genetic analyses, I (a) examined factors influencing sex ratio variation in gynodioecious plants (in which hermaphrodites and females coexist), (b) discovered variation in breeding system, (c) investigated density-dependence of seed production, and (d) documented genetic variation within and among populations close to the northern range margin in Europe. In a survey of 104 P. maritima populations, I documented considerable variation in sex ratio (range 0-70% females, median 6.3% females). As predicted, females were more frequently missing from small than from large populations, and the variance in sex ratio increased with decreasing population size. Among twelve populations sampled for seed production, the frequency of females was positively related to relative fecundity of females and negatively related to population size. The results suggest that the local sex ratio is influenced both by the relative fecundity of females and hermaphrodites, and by stochastic processes in small populations. A comparative field study showed that plant fecundity decreased with increasing distance to nearest pollen donor both within and among populations in an archipelago in southern Sweden, where self-incompatibility was confirmed in controlled crosses. In contrast, plant fecundity was overall higher and was not density-dependent in the Skeppsvik archipelago in northern Sweden, where controlled crosses showed that plants are self-compatible. The results were consistent with the prediction that evolution of self-fertility should reduce density-dependence of pollination success. I quantified the genetic structure within and among populations from eastern Sweden and western Finland based on variation at four polymorphic microsatellite loci. The genetic diversity was low in northern Sweden, which may be the result of a history of small population sizes and periods of frequent self-fertilization.

Les ataxies spastiques héréditaires forment une famille hétérogène de désordres qui ont des points communs avec les ataxies héréditaires et les paraplégies spastiques héréditaires. Un de ces éléments est une ataxie, soit une difficulté de coordination des membres souvent due à un dommage au cervelet. L’autre est une spasticité des membres inférieurs, souvent due à des dommages à la voie cortico-spinale. Une seule ataxie spastique à hérédité autosomique dominante a été rapportée dans la littérature, et il s’agit de SPAX1. À l’aide de trois familles de Terre-Neuve présentant ce phénotype, le locus a été identifié en 2002. Dans ce mémoire, c’est de la découverte du gène causal dont il est question. La mutation a été trouvée dans le gène VAMP1, qui encode la protéine synaptobrévine 1, une protéine synaptique impliquée dans l’exocytose des neurotransmetteurs. Il est aussi question de la caractérisation fonctionnelle de la mutation sur l’ARN et des conséquences possibles sur la protéine, concordant avec les symptômes de la maladie. / Hereditary spastic ataxias comprise a family of heterogeneous disorders resembling both hereditary ataxias and hereditary spastic paraplegias. The similar symptoms are ataxia, which is a problem with limb coordination due to cerebellar damage, and lower-limb spasticity due to corticospinal tract degeneration. Only one spastic ataxia inherited in an autosomal dominant fashion has been reported in the literature: SPAX1. The locus was identified in 2002 using three families from Newfoundland with the specific phenotype. This thesis reports the discovery of the causative mutation in the VAMP1 gene, which encodes VAMP1/synaptobrevin 1, a synaptic protein involved in neurotransmitter exocytosis. Experiments characterizing the effect of the mutation on RNA were conducted, leading to a possible molecular explanation of the symptoms.